共查询到20条相似文献,搜索用时 31 毫秒
1.
K Werkmeister F Wieland E Schweizer 《Biochemical and biophysical research communications》1980,96(1):483-490
A mixture of two pantetheine-free mutant fatty acid synthetases was dissociated and recombined to form a hybrid apoenzyme complex. the corresponding -mutants exhibit interallelic complementation when crossed with each other and the enzyme synthesized in the resulting diploid contains pantetheine and exhibits overall fatty acid synthetase activity. Accordingly, the hybrid apoenzyme formed could be activated to holo-fatty acid synthetase when incubated with coenzyme A and a partially purified yeast cell extract. The enzyme coenzyme A: fatty acid synthetase apoenzyme 4′-phosphopantetheine transferase has thus been identified in yeast. Further studies on the mechanism of fatty acid synthetase holoenzyme formation will now be possible. 相似文献
2.
Theodore A. Alston Harold J. Bright 《Biochemical and biophysical research communications》1982,105(2):560-566
L-Alanosine is an antineoplastic drug which is the 3-isonitramino analog of -aspartic acid. The drug is known to be metabolized to the corresponding 2-oxo acid. Unlike the parent amino acid, the 2-oxo acid is unstable under mild conditions. When the 2-oxo acid is generated by the aerobic action of -amino acid oxidase on -alanosine, the reaction mixture contains products capable of diazotizing sulfanilamide and of reducing ferricytochrome to ferrocytochrome . It is thus likely that, as expected from model reactions, the unstable 2-oxo acid derived from -alanosine decomposes into nitric oxide and other reactive free-radical species. Enzymatically promoted production of highly cytotoxic nitric oxide may pertain to the biological activity of the antibiotic. The reaction should prove extrapolable to the design of other enzyme-activated cytotoxic agents. 相似文献
3.
Partial acid hydrolyzates of the extracellular polysaccharide from Porphyridiunm cruentum yield three disaccharides and two uronic acids. These constitute all of the uronic acid in the polymer. The novel disaccharides are 3-O-(α--glucopyranosyl- uronic acid)--galactose, 3-O-(2-O-methyl-ca-glucopyranosyluronic acid)-- galactose, and 3-0-(2-0-methyl-a--glucopyranosyluronic acid)--glucose. The polyanion of high molecular weight contains - and -galactose, xylose, -glucose, -glucuronic acid and 2-O-methyl-D-glucuronic acid, and sulfate in molar ratio (relative to -glucose) of 2.12:2.42:1.00:1.22:2.61. Preliminary periodate-oxidation studies suggest that the hexose and uronic acids are joined to other residues by ( 1→3) glycosidic linkages. About one-half of the xylose residues are (1→3)-linked. 相似文献
4.
Yukio Imanishi Hiroyuki Ohnishi Yutaka Hashimoto 《International journal of biological macromolecules》1981,3(2):97-104
The enantiomer selection in the nucleophilic addition reaction of optically active amines such as α-amino acid esters to phenylalanine and in as a solvent has been investigated. Stereoselectivity between the amines and the was found to change markedly according to the reaction conditions. This experimental finding is in contrast to the idea hitherto accepted that in the nucleophilic addition-type polymerization of α-amino acid the growing chain end reacts preferentially with one of the enantiomorphic having the same configuration, and indicates the importance of the investigation of stereoselectivity in the polymerization using suitable model reactions. Most acid esters reacted preferentially with , and this type of stereoselectivity increased with the of and with increasing bulkiness of the Cα substituent of α-amino acid esters (alanine < norleucine < leucine < valine). The relationship observed between the stereoselectivity and the structures of amines and was explained satisfactorily in terms of the transition state model in which the interaction of nitrogen and α-amino acid ester carbonyl as well as the interaction of carbonyl and α-amino acid ester nitrogen was taken into account. ethyl ester did not show enantiomer selectivity toward phenylalanine , but reacted preferentially with . for the reaction of proline ester with a transition-state model was proposed, which was different from the transition state model proposed for other α-amino acid esters. Some experiments were carried out to examine the transition-state models proposed. The implications of the present investigation in stereoselectivity in the nucleophilic addition-type polymerization of hitherto reported are discussed. 相似文献
5.
Theodore Dashman 《Life sciences》1980,27(15):1415-1422
The enol-ether amino acid, L-2-amino-4-methoxy--butenoic acid (AMTB) is an inhibitor of porphobilinogen synthase (PBG synthase) when added prior to the addition of the substrate δ-aminolevulinic acid. The inhibition of PBG synthase by several stereoisomers and analogues of AMTB was investigated to determine those structural features of AMTB which may be necessary for inhibition. The D- isomer was also an inhibitor after preincubation, whereas the L- isomer inhibited with or without preincubation. The amino acid analogues, DL-vinylglycine, DL-2-aminobutanoic acid, the reduced form of L-2-amino-4-methoxy--3-butenoic acid, L-2-amino-4-(2-aminoethoxy)--3-butenoic acid and its reduced congener did not inhibit PBG synthase even with preincubation. This structure activity relationship indicates that the double bond and methoxy moiety of L-2-amino-4-methoxy--3-butenoic acid are probably required for inhibition.Heme, when preincubated with PBG synthase, was an inactivator of the enzyme. However, when both L-2-amino-4-methoxy--3-butenoic acid and heme were simulatneously preincubated with PBG synthase, inactivation of the enzyme was greater than with either compound separately. The possibility of multiple catalytic sites was suggested by the use of multiple inhibition kinetics in the presence of heme and L-2-amino-4-methoxy--3-butenoic acid. 相似文献
6.
Occurrence of cis-7-tetradecenoic acid in the envelope phospholipids of Escherichia coli K12 总被引:1,自引:0,他引:1
We have isolated a tetradecenoic acid from and have identified this new acid as -7-tetradecenoic by its 13C nuclear magnetic resonance spectrum. This identification was confirmed by conventional structural studies. The acid is a component of the phospholipids of and comprises about 15% of the total phospholipid unsaturated fatty acid. 相似文献
7.
S G Batrakov A G Panosyan G A Kogan L D Bergelson 《Biochemical and biophysical research communications》1975,66(2):755-762
The circular dichroism spectra of natural glycerophospholipids and synthetic 1--phosphatidic acid were recorded. 3--phosphatidic acid derivatives were found to show a positive Cotton effect, while 1--phosphatidic acid revealed a negative Cotton effect. The results are interpreted in terms of the carboxyl sector rule. By this method phospholipase D was shown to produce stereospecifically 3--phosphatidyl-1--glycerol when incubated with egg yolk lecithin and exess of glycerol. 相似文献
8.
Interaction of the lectin limulin with capsular polysaccharides from Neisseria meningitidis and Escherichia coli 总被引:1,自引:0,他引:1
E F McSweegan T G Pistole 《Biochemical and biophysical research communications》1982,106(4):1390-1397
The lectin limulin from the serum of the horseshoe crab binds to -acetylneuraminic acid and 2-keto-3-deoxyoctonate residues. These interactions were examined using capsular polysaccharides from strains of and . Our findings indicate that limulin has greatest reactivity with homopolymers of -acetylneuraminic acid as compared with heteropolymers of either sugar. Polysaccharides with α(2→9) ketosidic linkages were most efficient in precipitating this lectin. Finally, -acetylated homopolymers of -acetylneuraminic acid were more reactive than their -acetyl-negative counterparts. 相似文献
9.
Studies of the influence of fatty acids, which were the component of intestinal mucosal lipids, on the permeability of several drugs across bilayer lipid membranes generated from egg phosphatidylcholine and intestinal lipid have been pursued. The permeability coefficients of acid, salicylic acid and acid (anionic-charged drug) increased when fatty acids such as lauric, stearic, oleic, linoleic and linolenic acid were incorporated into the bilayer lipid membranes generated from phosphatidylcholine. In the presence of methyl linoleate and oleyl alcohol, no enhancing effect on acid transfer was obtained. The effect of fatty acids was more marked at pH 6.5 than at pH 4.5. In contrast, upon the addition of fatty acids to intestinal lipid membranes which originally contained fatty acids, the permeability coefficient of acid tended to decrease, though the permeability through intestinal lipid membranes was larger than that of phosphatidylcholine membranes. The permeability of acid across bilayer lipid membranes from intestinal phospholipids was significantly decreased to about equal that of phosphatidylcholine membranes, and reverted to the value of intestinal lipid membranes when fatty acids were added to intestinal phospholipids. It seemed reasonable to assume that free fatty acids in the intestinal neutral lipid fraction could contribute to the increase in the permeability of acid. On the basis of above results, possible mechanisms for good absorbability of weakly acidic drugs from the intestine are discussed. 相似文献
10.
Biosynthesis of glycerol teichoic acid in Bacillus cereus: formation of linkage unit disaccharide on a lipid intermediate 总被引:5,自引:0,他引:5
N Murazumi Y Sasaki J Okada Y Araki E Ito 《Biochemical and biophysical research communications》1981,99(2):504-510
A tunicamycin-like antibiotic 24010 at a concentration of 1 μg/ml selectively inhibited the synthesis of glycerol teichoic acid of cell walls in AHU 1030. Incubation of membranes of this strain with -acetylglucosaminyl pyrophosphorylundecaprenol and UDP--acetylmannosamine led to formation of a glycolipid having a saccharide moiety identical with the cell wall teichoic acid linkage unit, -acetylmannosaminylβ(1→4)--acetylglucosamine. The membranes also catalyzed transfer of glycerol phosphate units from CDP-glycerol to this disaccharide-linked lipid. Thus the biosynthesis of the cell wall glycerol teichoic acid in this strain seems to involve the disaccharide-linked lipid as an intermediate. 相似文献
11.
Oxidative phosphorylation, active transport of proline, aerobic- and ATP-driven proton translocation and transhydrogenation of NADP+ by NADH, occurred in lipoic acid-deficient cells or vesicles of a lipoic acid auxotroph of , W1485 lip 2. Addition of lipoic acid had little effect on these processes. Tributyltin chloride, which has been proposed to inhibit oxidative phosphorylation by reaction with lipoic acid (Cain , Biochem. J. (1977) , 593), was an effective inhibitor of aerobic and ATP-dependent proton translocation and transhydrogenation in lipoic acid-deficient vesicles from this organism. Our results do not support the proposal of Partis (FEBS Lett. (1977) , 47) that lipoic acid is involved in the energy transducing processes associated with the membrane of . 相似文献
12.
Two valine-sensitive acetohydroxy acid synthase activities were separable from K-12 cells by virtue of their different affinities for DEAE-cellulose eluted with a KC1 gradient. These activities appeared to be independent from a valine-resistant cryptic component expressed only in regulatory mutants. The properties of the first and second activity were coincident to those of extracts of and mutants, respectively. These data prove that the and gene products exist in the cell as physically distinct acetohydroxy acid synthase isoenzymes. 相似文献
13.
M S Kilberg H N Christensen M E Handlogten 《Biochemical and biophysical research communications》1979,88(2):744-751
The rapid transport of -cysteine into isolated rat hepatocytes escapes detectable inhibition by 2-(methylamino)-isobutyric acid at levels up to 50 mM. The system transporting cysteine instead is convincingly similar to the system described for the Ehrlich cell in structural and steric specificity and in pH sensitivity. The Na+-dependent uptake of 2-aminoisobutyric acid is almost evenly divided between Systems and , showing better accommodation of its two α-methyl groups by than in the Ehrlich cell. The hepatocyte system tolerates Li+-for-Na+ substitution better than does System , although the tolerance depends on amino acid structure. Adaptive regulation and insulin and glucagon stimulation were not seen under conditions producing these effects for System . 相似文献
14.
S Nasu F D Wicks S Sakakibara R K Gholson 《Biochemical and biophysical research communications》1978,84(4):928-935
Two proteins (A and B) from are required for the synthesis of the NAD precursor quinolinate from aspartate and dihydroxyacetone phosphate. Mammalian liver contains a FAD linked protein which replaces B protein for quinolinate synthesis. D-aspartic acid but not L-aspartic acid is a substrate for quinolinic acid synthesis in a system composed of the B protein replacing activity of mammalian liver and A protein. In contrast the B protein- A protein quinolinate synthetase system requires L-aspartic acid as substrate. The previous report that L-aspartate was a substrate in the liver- system was due to contamination of commercially available [14C]L-aspartate with [14C]D-aspartate. These and other observations suggest that liver B protein is D-aspartate oxidase and B protein is L-aspartate oxidase. 相似文献
15.
A complete titration of phosphatidic acid bilayer membranes was possible for the first time by the introduction of a new anaologue, acid, which has the advantage of a high chemical stability at extreme pH values. The synthesis of this phosphatidic acid is described and the phase transition behaviour in aqueous dispersions is compared with that of three ester phosphatidic acids; acid, 1,3-dimyristoylglycerol-2-phosphoric acid and acid.The phase transition temperatures () of aqueous phosphatidic acid dispersions at different degrees of dissociation were measured using fluorescence spectroscopy and 90° light scattering. The values are comparable to the melting points of the solid phosphatidic acids in the fully protonated states, but large differences exist for the charged states.The vs. pH diagrams of the four phosphatidic acids are quite similar and of a characteristic shape. Increasing ionisation results in a maximum value for the transition temperatures at pH 3.5 (). The regions between the first and the second of the phosphatidic acids are characterised by only small variations in the transition temperatures (extended plateau) in spite of the large changes occurring in the surface charge of the membranes. The slope of the plateau is very shallow with increasing ionisation. A further decrease in the H+ concentration results in an abrupt change of the transition temperature. The slope of the vs. pH diagram beyond becomes very steep. This is the 相似文献
16.
Cytochrome LM2 was reconstituted by the cholate-dialysis method into vesicles containing a mixture of either phosphatidylcholine or phosphatidylethanolamine with up to 50 mol% of phosphatidic acid. Phase transition curves in the presence or absence of cytochrome were obtained from electron paramagnetic resonance experiments by measuring the partitioning of 2,2,6,6-tetramethylpiperidine-1-oxyl. Protein-free phospholipid vesicles exhibit a phase separation into domains of gel phase enriched in phosphatidic acid in a surrounding fluid matrix containing mainly phosphatidylcholine. The phase transition of the phosphatidic acid domains disappeared following incorporation of cytochrome into the bilayers. In contrast, in vesicles containing mixtures of egg-phosphatidic acid and dimyristoyl phosphatidylcholine, the phase transition of the domains enriched in dimyristoyl phosphatidylcholine was less sharp than in the corresponding vesicles containing cytochrome . The results of both of these experiments could be explained by a redistribution of the mol fraction of the two phospholipids in the gel phase due to preferential binding of the egg-phosphatidic acid to the cytochrome . For comparison, incorporation of cytochrome into uncharged vesicles of dimyristoyl phosphatidylcholine and egg-phosphatidylethanolamine did not alter the 相似文献
17.
Acyltransferases are present in microsomes from alveolar type II cell adenomas (produced by urethan injections) that transfer palmitic acid in the presence of CoA, ATP, and Mg++ to -glycerol-3-P to form phosphatidic acid, to dihydroxyacetone-P to form acyldihydroxyacetone-P, and to 1-acyl--glycero-3-phosphocholine to form 3--phosphatidylcholine. The data clearly demonstrate that the microsomal preparations can catalyze significant incorporation of palmitic acid into the 2-position of the disaturated species of 3-sn-phosphatidylcholine independently of phosphatidic acid formation as evidenced by the fact that -glycerol-3-P and calcium ions (which inhibit choline phosphotransferase) did not influence the incorporation of palmitic acid into the main surfactant lipid. Thus, a deacylation-acylation reaction involving 2-lysophosphatidylcholine appears to be an important pathway for the synthesis of surfactant lipid in alveolar type II cells; the control of acyl specificity at the 2-position is determined by the relative concentrations of the coparticipating substrates, l-palmitoyl--glycero-3-phosphocholine and palmitoyl-CoA. 相似文献
18.
Yuji Kamiya Akira Sakurai Saburo Tamura Nobutaka Takahashi Keiko Abe Eiko Tsuchiya Sakuzo Fukui Chieko Kitada Masahiko Fujino 《Biochemical and biophysical research communications》1978,83(3):1077-1083
Rhodotorucine is a peptidyl factor which induces mating tube formation in . The amino acid sequence of the factor was determined by Edman degradation and enzymatic hydrolysis. Rhodotorucine was shown to contain a lipophilic amino acid, S-farnesyl cysteine, at C-terminus by proton magnetic resonance, mass spectrometry and chemical synthesis. We proposed the following structure for rhodotorucine . H-Tyr-Pro-Glu-Ile-Ser-Trp-Thr-Arg-Asn-Gly-Cys(S-farnesyl)-OH 相似文献
19.
Giovanna Grimaldi John Guardiola 《Biochemical and biophysical research communications》1981,101(4):1233-1240
The mutation (obtained by phage mediated mutagenesis) affects the sensitivity to valine inhibition of the acetohydroxy acid synthase III isoenzyme of K-12, as shown by constructing multiple mutants containing the mutation and only one of the genes for the expression of the three acetohydroxy acid synthase isoenzymes, at once. The mutation is dominant. This suggests that the phenotype of mutation is not caused by inactivation of a gene concerned with the expression of the AHASIII enzyme, consequent to prophage insertion into that locus. 相似文献
20.
Mary Ann Dombrink-Kurtzman William E. Dick Kermit A. Burton Martin C. Cadmus Morey E. Slodki 《Biochemical and biophysical research communications》1983,111(3):798-803
Evidence is presented for the presence of a new lectin activity in soybean seeds [ (L.) Merrill] that has specificity towards the 4-O-methyl--glucurono--rhamnan exopolysaccharide produced by certain strains of . Bacterial agglutination and precipitin reactions revealed the lectin activity in phosphate-buffered saline extracts of seeds of all cultivars tested, including the “lectinless” varieties. Reaction of such extracts with carbohydrate haptens demonstrated that the specificity of the binding was towards 4--methyl--glucuronic acid, -glucuronic acid and their methyl glycosides. 相似文献