Age estimation and lead�Cradium dating of Antarctic toothfish (Dissostichus mawsoni) in the Ross Sea

Antarctic toothfish (Dissostichus mawsoni) are the target of an important commercial fishery in the Southern Ocean, yet age data used for management have not been comprehensively tested for accuracy. In this study, Antarctic toothfish were aged using counts of otolith growth zones based on criteria established for Patagonian toothfish, D. eleginoides, a closely related species. To validate these ages, the radioactive disequilibrium of lead-210 and radium-226 in otolith cores was measured and used as an independent chronometer to accurately determine age across the range of fish caught in large numbers by the fishery. Growth-zone counts indicated Antarctic toothfish live to at least 39 years of age, and were in close agreement with the chronometer, validating the age estimation criteria and the accuracy of age estimates. Von Bertalanffy growth function parameters indicated Antarctic toothfish were relatively slow-growing (k = 0.111), especially in relation to their maximum size (L _∞ = 158.9 cm).     

Fisheries assessment of Chwaka Bay (Zanzibar) – following a holistic approach

This study investigates the typical East African multispecies fishery of Chwaka Bay (Zanzibar) by assessing growth, mortality, exploitation and stock size of six key target species of the fishery. Analyses are based on monthly length–frequency data collected from January to June and September to December in 2014 from the three main landing sites surrounding the bay. Estimated von Bertalanffy growth parameters using the ELEFAN I routine as implemented in FiSAT II varied among species from 27.6 to 64.4 cm (L_∞) and 0.24 to 0.8 per year (K). Current exploitation rates compared to biological reference points calculated from yield‐per‐recruit analysis for five of the six key species indicate that Siganus sutor, Lethrinus borbonicus and Lethrinus lentjan are harvested beyond maximum sustainable levels (E_max). While juvenile retention rates of these three species are >80% and current L_c‐values are lower than L_opt, fishing mortalities for Siganus sutor and Lethrinus borbonicus are highest for specimens above length at first maturity. Two management measures are discussed: (i) an increase in mesh size; and (ii) closure of the destructive dragnet fishery. The first option seems feasible only if the radius of the fishery were increased to capture larger specimens outside the shallow bay area. The second option would leave approximately 550 fishermen unemployed. Reallocating dragnet fishing effort to other gears would lead to a substantial increase in the number of boats, which might create use conflicts over the limited fishing area. The general status of the fishery in Chwaka Bay is considered as representing a ‘full exploitation to over‐exploitation scenario’, with no scope for expansion.     

Biology of the kingsoldier bream (Argyrops spinifer, Forsskål 1775; Sparidae), from the Arabian Sea, Oman

It is recognized by the FAO that management of fish resources in the western Indian Ocean is complicated by the lack of data on the basic biology and landing statistics for exploited fish species. In this paper data are presented on the population biology of kingsoldier bream, Argyrops spinifer (Forsskål 1775), in the Arabian Sea with a view to contributing towards the development of management plans for its sustainable exploitation. Samples were obtained from the Omani artisanal hand‐line fishery catch, January 2001–December 2002. Total length (L_T) of fish sampled ranged from 20 to 68 cm (males) and 25 to 70 cm (females). The male : female ratio was similar up to 55 cm L_T but the larger size‐classes were composed predominately of male fish. L_T/carcass weight (W_C) relations for male and female fish were similar and the combined data produced an equation W_C = 0.00005 × L_T^2.67. Marginal increment analysis indicated an annual cycle of opaque and translucent ring formation in otoliths that was related to the change in surface water temperature during the annual monsoon period. The opaque zone was completed in May and the translucent zone began to form in June. Female and male fish age ranged between 2 and 25 years. Both males and females exhibited similar asymptotic growth patterns; the combined von Bertalanffy growth function was L_t = 64.6(1 ? e^{[?0.142(t + 0.489)]}). Gonadosomatic indices and gonad condition of male and female fish indicated that the spawning season occurred between September and January. Size (L₅₀) and age at first maturity (A₅₀) were estimated to be 36.5 cm L_T and 5.0 years for males and 37.2 cm L_T and 5.6 years females, respectively. The results of this study provide fundamental information on the biology and population dynamics of A. spinifer in the Arabian Sea that can be used in management models for the continued sustainable exploitation of this species in the Omani demersal coastal fisheries.     

Age and growth of black seabream Acanthopagrus schlegelii (Sparidae) in Hong Kong and adjacent waters of the northern South China Sea

Age and growth of the black seabream Acanthopagrus schlegelii (family Sparidae) from the northern South China Sea (NSCS) were studied by reading growth rings in sectioned sagittal otoliths. Ring formation frequency was determined to be annual by using marginal increment analysis. The von Bertalanffy growth function parameters were estimated as: L_∞ = 43.7 cm L_S; K =0.22 year; t₀ = ?1.59 years. Functional males are significantly younger than functional females, with sexually transitional individuals between the modal ages of males and females supporting protandry in this species. Males become sexually mature within 1 year and 50% age at sex change is at 2 years. The maximum age recorded for both males and females sampled was 9 years which is lower than for conspecifics elsewhere and may reflect high fishing pressure in the study area when compared with conspecifics in other areas or could reflect latitudinal effects. Otolith mass was significantly and positively related to age, providing a cheap and quick alternative method for approximating age. Acanthopagrus schlegelii is a relatively fast‐growing and rapidly maturing species attaining a similar asymptotic length to conspecifics. A need for fishery management is indicated to protect both young juveniles and older adults, especially females, to increase reproductive output and safeguard fishery production.     

Size selectivity of three diamond mesh codends for the European hake (Merluccius merluccius) and the tub gurnard (Trigla lucerna) in the Sea of Marmara, Turkey

The effects of an increase in the codend mesh size from 44 to 56 and 80 mm on size selectivity of the two by‐catch species (the European hake Merluccius merlucius and the tub gurnard Trigla lucerna) commonly captured in the shrimp fishery in the Sea of Marmara, were evaluated. The parameters of the logistic selectivity model were estimated by maximum likelihood. Length at 50% retention, L₅₀, was found to increase with mesh size for both species. The L₅₀ values of 44, 56 and 80 mm were, respectively, 19.8, 21.5 and 33.4 cm for European hake, 17.9, 17.7 and 25.6 cm for tub gurnard. The main conclusions are that 44 mm and 56 mm diamond mesh codends retain a considerable amount of undersized hake and tub gurnard as by‐catch in the shrimp fishery. With 80 mm mesh size codend, most juvenile European hake are released. For the tub gurnard, however, even this mesh size does not provide a good enough selection. From the biological point of view, it is concluded that the legal 44 mm diamond mesh codend does not provide satisfactory selection for European hake and the tub‐gurnard in trawl fisheries of the Sea of Marmara.     

Non‐zero‐sum neutrality test for the tropical rain forest community using long‐term between‐census data

For community ecologists, “neutral or not?” is a fundamental question, and thus, rejecting neutrality is an important first step before investigating the deterministic processes underlying community dynamics. Hubbell''s neutral model is an important contribution to the exploration of community dynamics, both technically and philosophically. However, the neutrality tests for this model are limited by a lack of statistical power, partly because the zero‐sum assumption of the model is unrealistic. In this study, we developed a neutrality test for local communities that implements non‐zero‐sum community dynamics and determines the number of new species (N _sp) between observations. For the non‐zero‐sum neutrality test, the model distributed the expected N _sp, as calculated by extensive simulations, which allowed us to investigate the neutrality of the observed community by comparing the observed N _sp with distributions of the expected N _sp derived from the simulations. For this comparison, we developed a new “non‐zero‐sum N _sp test,” which we validated by running multiple neutral simulations using different parameter settings. We found that the non‐zero‐sum N _sp test rejected neutrality at a near‐significance level, which justified the validity of our approach. For an empirical test, the non‐zero‐sum N _sp test was applied to real tropical tree communities in Panama and Malaysia. The non‐zero‐sum N _sp test rejected neutrality in both communities when the observation interval was long and N _sp was large. Hence, the non‐zero‐sum N _sp test is an effective way to examine neutrality and has reasonable statistical power to reject the neutral model, especially when the observed N _sp is large. This unique and simple approach is statistically powerful, even though it only employs two temporal sequences of community data. Thus, this test can be easily applied to existing datasets. In addition, application of the test will provide significant benefits for detecting changing biodiversity under climate change and anthropogenic disturbance.     

Reproductive biology of black seabream Acanthopagrus schlegelii,threadfin porgy Evynnis cardinalis and red pargo Pagrus major in the northern South China Sea with consideration of fishery status and management needs

The reproductive biology of three commercially significant seabream species (family: Sparidae) Acanthopagrus schlegelii, Evynnis cardinalis and Pagrus major, taken from Hong Kong and adjacent northern South China Sea (SCS) waters, were investigated for their sexual patterns, spawning seasons, length at maturity and exploitation in relation to their conservation and management status. Histological analysis showed E. cardinalis and P. major to be functionally gonochoristic, the latter having a bisexual juvenile stage and being a rudimentary hermaphrodite. Acanthopagrus schlegelii is a protandric hermaphrodite. Standard length (L_S) at 50% sex change for A. schlegelii is 291 mm. L_S at 50% female maturity for E. cardinalis and P. major are 117 and 332 mm, respectively. For all three species, the spawning period falls between November and March. The study highlights geographical differences in reproductive biology among the species and a paucity of fishery or other population‐related data. While heavy fishing pressure, life‐history characteristics and absence of effective management throughout the geographic ranges of these species make them susceptible to overfishing, they nonetheless appear to be generally more resilient than many other taxa that comprise the multi‐species fisheries of the region, possibly due to their relatively rapid sexual maturation and spatial movement patterns. Overall, however, little is known of the biology, fishing history and current fishery status of sparids in general in the northern SCS and the current study is one of the first to examine such aspects of this family in the region and to consider appropriate management options.     

Mass-balanced trophic and loop models of complex benthic systems in northern Chile (SE Pacific) to improve sustainable interventions: a comparative analysis

The capacity to withstand perturbations and the propagation of direct and indirect effects of harvesting were explored in seagrass and sand–gravel subtidal subsystems of northern Chile using Ecopath II, Ecosim and Loop Analysis theoretical frameworks. The relative Ascendancy and Redundancy from Ecopath II and Ecosim models and the holistic stability measure (F _n ) given by Loop Analysis, all suggest that the sand–gravel subsystem is the more resistant and its fishery more sustainable. This outcome is relevant since the theoretical frameworks used are based on different assumptions but arrive at similar conclusions. If the fishery is included in the qualitative ecological models, which were found locally stable only when the fishery is self-controlled. While this conclusion is not new, its relevance here is that it emerges from qualitative multispecies modelling (Loop Analysis). Based on our model predictions, the biomass removal of the sea star Meyenaster gelatinosus would increase the standing stock of the commercial scallop Argopecten purpuratus only in the seagrass habitat, whereas the same man-made intervention may have an opposite effect in the sand–gravel habitat. We recommend to enunciate holistic models based on the theoretical framework used in the current work, which explore the reality from different perspectives assuming different givens. Based on these holistic explorations alternative and complementary management scenarios could be designed. Handling editor: T. P. Crowe     

Disentangling the effects of recombination,selection, and demography on the genetic variation at a major histocompatibility complex class II gene in the alpine chamois

The major histocompatibility complex (MHC) harbours some of the most polymorphic loci in vertebrate genomes. MHC genes are thought to be subject to some form of balancing selection, most likely pathogen‐mediated selection. Hence, MHC genes are excellent candidates for exploring adaptive processes. In this study, we investigated the genetic variation at exon 2 of the DRB class II MHC locus in 191 alpine chamois (Rupicapra rupicapra) from 10 populations in the eastern Alps of Italy. In particular, we were interested in distinguishing and estimating the relative impact of selective and demographic factors, while taking into account the confounding effect of recombination. The extremely high d_n/d_s ratio and the presence of trans‐species polymorphisms suggest that a strong long‐term balancing selection effect has been operating at this locus throughout the evolutionary history of this species. We analysed patterns of genetic variation within and between populations, and the mitochondrial D‐loop polymorphism patterns were analysed to provide a baseline indicator of the effects of demographic processes. These analyses showed that (i) the chamois experienced a demographic decline in the last 5000–30 000 years, most likely related to the postglacial elevation in temperature; (ii) this demographic process can explain the results of neutrality tests applied to MHC variation within populations, but cannot justify the much weaker divergence between populations implied by MHC as opposed to mitochondrial DNA; (iii) similar sets of divergent alleles are probably maintained with similar frequencies by balancing selection in different populations, and this mechanism is also operating in small isolated populations, which are strongly affected by drift.     

Effects of Management on Economic Profitability of Forest Biomass Production and Carbon Neutrality of Bioenergy Use in Norway Spruce Stands Under the Changing Climate

We analyzed the effects of management on the economic profitability of forest biomass production and carbon neutrality of bioenergy use in Norway spruce (Picea abies L. Karst) stands under the changing climate. We employed a forest ecosystem model and life cycle assessment tool. In particular, we studied the effects of thinning, nitrogen fertilization, and rotation length on: (1) the production of timber and energy biomass, and its economic profitability (net present value), (2) carbon stock in the forest ecosystem and carbon balance in forestry, and (3) carbon dioxide (CO₂) emissions from the use of biomass in energy production. Results showed that the current Finnish baseline management with and without nitrogen fertilization resulted in the highest mean annual timber production and net present value (NPV) for long rotations (60 to 80 years), regardless of climate scenario. Mean annual production of energy biomass was enhanced by increasing stocking by 20–30 % compared to the baseline management, and/or use of nitrogen fertilization. Such management gave lower CO₂ emissions per unit of energy compared to the baseline management, as the carbon stock in the forest ecosystem and the carbon balance in forestry increased. Overall, the carbon neutrality and net present value were, on average, the highest in the baseline management or with a 20 % increase in stocking, with nitrogen fertilization and 60- to 80-year rotation lengths, regardless of the climate applied. However, it was not possible to simultaneously maximize the NPV of forest biomass production and the carbon neutrality of bioenergy use.     

Genetic variability of the stable fly assessed on a global scale using amplified fragment length polymorphism

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), is a blood‐feeding, economically important pest of animals and humans worldwide. Improved management strategies are essential and their development would benefit from studies on genetic diversity of stable flies. Especially if done on a global scale, such research could generate information necessary for the development and application of more efficient control methods. Herein we report on a genetic study of stable flies using amplified fragment length polymorphism, with samples of 10–40 individuals acquired from a total of 25 locations in the Nearctic, Neotropic, Palearctic, Afrotropic and Australasian biogeographical regions. We hypothesized that genetic differentiation would exist across geographical barriers. Although F_ST (0.33) was moderately high, the G_ST (0.05; representing genetic diversity between individuals) was very low; N_m values (representing gene flow) were high (9.36). The mismatch distribution and tests of neutrality suggested population expansion, with no genetic differentiation between locations. The analysis of molecular variance (AMOVA) results showed the majority of genetic diversity was within groups. The mantel test showed no correlation between geographic and genetic distance; this strongly supports the AMOVA results. These results suggest that stable flies did not show genetic differentiation but are panmictic, with no evidence of isolation by distance or across geographical barriers.     

Reproductive ecology of the female pink cusk-eel (Genypterus blacodes): evaluating differences between fishery management zones in the Chilean austral zone

The pink cusk-eel (Genypterus blacodes), a benthic-demersal fish confined to the southern hemisphere, supports an important commercial fishery in Chile where it is exploited over an extensive geographic area. Although the fishery was originally divided into a northern (41º28′–47º00′S) and southern (47º00′–57º00′S) zone for the purposes of fisheries management, recent studies have reported significant differences in life history parameters between these zones. Individuals from the southern zone reached larger asymptotic sizes and possessed higher survival rates compared to the northern zone. We estimate and compare the gonadosomatic index (GSI), shape of the maturity ogive, and length at 50 % maturity (L _50%) of female G. blacodes between management zones and across time using biological data collected from the industrial fleet between 1985 and 2009. Females in the northern zone had higher monthly mean GSI than females in the southern zone. Our analyses also revealed L _50% to be significantly higher in the southern zone than in the northern zone from 1985 to 2009. The significant differences in life-history traits between fishery management zones agree with the trade-offs predicted by Charnov’s life history theory. Together these results provide additional support for the hypothesis that two separate stocks exist and suggest that females from the northern zone have developed a life-history strategy, which favours early maturation and a proportionally greater investment in reproduction than females from the southern zone.     

Genetic structure,phylogeography, and demography of Anadara tuberculosa (Bivalvia) from East Pacific as revealed by mtDNA: Implications to conservation

Wild populations of the pustulose ark, Anadara tuberculosa (Bivalvia), an emblematic species of the East Pacific mangrove ecosystem declined in South American countries (Colombia, Ecuador, and Peru) mainly due to overharvesting and habitat loss or degradation. Understanding the genetic aspects of geographic variations and population structure of A. tuberculosa, currently unknown, appears as a priority to fishery authorities in order to elaborate integrated and collaborative conservation policies for fishery management, aquaculture, and stock enhancement programs. We used mtDNA sequence data to investigate haplotype diversity, genetic structure, and demography of A. tuberculosa. Results indicate genetic homogeneity of populations distributed north and south of the equator, respectively. However, statistically significant differentiation emerged between northern and southern populations with pairwise ф_ST values ranging between 0.036 and 0.092. The oceanic current system acting in the area (Panama Current and Humboldt Current) might play a role in limiting the larval dispersal of the species, still poorly understood. Demography reconstruction supported recent population expansion, possibly started after last glacial maximum. Our results would suggest separate and independent management of populations north and south of the equator.     

Population genomics study for the conservation management of the endangered shrub Abeliophyllum distichum

Natural monuments are IUCN Category III protected areas that play an important role in biodiversity conservation as they provide species refuge and allow species migration. Despite their status, natural monuments are often confined to cultural and fragmented landscapes due to anthropogenic land-use demands. In this population genomic study, we surveyed 11 populations of the endemic shrub Abeliophyllum distichum Nakai (Oleaceae), including five natural monument habitats, covering its range-wide distribution in South Korea. Using 2,254 SNPs as markers, our results showed a mean expected heterozygosity (He) of 0.319, with populations in the central distribution showing significantly higher He than those at the periphery. There was no significant heterozygote deficiency and inbreeding among studied populations overall (F_{IS =} ?0.098), except for a single natural monument population (GS-NM147). Population structure and differentiation was moderate to high (F_ST?=?0.196), while recent gene flow between populations appeared weak, which can be attributed to the fragmented distribution and the outcrossing mating system of the heterostylous plant. Based on these findings, we provide suggestions for the population conservation and management of this endangered species.

     

Using cross-dating techniques to validate ages of aurora rockfish (<Emphasis Type="Italic">Sebastes aurora</Emphasis>): estimates of age,growth and female maturity

Since fishery management regulations have shifted much of the groundfish trawl effort in the northeastern Pacific from the continental shelf to the slope, fishery impacts on unassessed demersal slope rockfish species like the aurora rockfish (Sebastes aurora) may have increased. Understanding the life history of these species is a critical first step in developing management strategies to protect them from overharvest. In this study we employ cross-dating methods to validate the annual periodicity of growth increments and investigate the age, growth and maturity of aurora rockfish, a species for which life history information is quite limited. Specimens were collected on an opportunistic basis from Oregon commercial landings and from research cruises, over the years 2003–2006. Age was estimated for 438 individuals using otoliths processed via the break-and-burn method. The maximum estimated age was 118 years for females (n = 324) and 81 years for males (n = 114). The von Bertalanffy growth function showed that males grow faster and reach a smaller maximum size than females (males: L _inf = 34, K = 0.09, t ₀ = −1.9; females: L _inf = 37, K = 0.06, t ₀ = −5.5), though both sexes demonstrate relatively slow growth. Visual assessment of ovaries showed that the aurora rockfish is a synchronous spawner with parturition occurring in May and June off Oregon. Female age and length at 50% maturity were calculated at 12.6 years and 26 cm, respectively (n = 307). Maturity and age data provided evidence for a protracted adolescence in this species.     

Reproductive biology of female humpback whitefish Coregonus pidschian in the Chatanika River,Alaska

Humpback whitefish Coregonus pidschian are found throughout Alaska and support both subsistence and personal‐use fisheries. Long‐term sustainability of these fisheries requires an understanding of life‐history characteristics and reproductive biology to provide fisheries managers with the tools required for science‐based management. The objective of this study was to examine the reproductive characteristics of humpback whitefish in the Chatanika River, Alaska, from late August through September 2008. Absolute fecundity, relative fecundity, gonadosomatic index (GSI), relative egg size, and age were examined for 60 female humpback whitefish. Mean absolute fecundity was 45 000 eggs female^?1 (range, 11 747–108 426 eggs female^?1) and was positively related to both fork length (r² = 0.74) and wet weight (r² = 0.83). Gonadosomatic index values averaged 15.5% (range, 6.4–23.7%). Relative fecundity was positively related to GSI (r² = 0.76) and showed a positive relationship with age until ages 15–20 before declining thereafter. Relative egg size was inversely related to GSI (r² = 0.38) and age (r² = 0.22) for female humpback whitefish. The results of our study provide information that will allow for better management of the personal use fishery for humpback whitefish in the Chatanika River and increase our understanding of the reproductive biology of this species throughout its geographic distribution.     

Elasmobranch species richness,fisheries, abundance and size composition in the Azores archipelago (NE Atlantic)

ABSTRACT

Elasmobranchs are a vulnerable resource, more susceptible to overfishing than most teleosts, and their assessment is complicated due to a general lack of information about their fisheries, biology and ecology. This study aimed to analyse all fishery and survey data available for elasmobranchs caught over the past c. 25 years around the Azores (NE Atlantic) to provide a baseline information, which can be used to inform stock assessment and management strategies. Elasmobranch species covered pelagic, benthopelagic and demersal habitats, from shallow to deep-water strata in areas around the islands and seamounts. These species are taken accidentally as by-catch of three main fisheries: swordfish fishery, black scabbardfish fishery and demersal bottom longline fishery. The latter represents one of the most important fishing activities in the Azores, and frequent elasmobranch by-catches include Raja clavata, Galeorhinus galeus, Deania calcea, D. profundorum, Etmopterus pusillus and E. spinax. A slight reduction in the abundance indices of these species was observed, despite the implemented technical measures (e.g. minimum size, zero catch). Little is known about resource dynamics for the Azorean region and no analytical assessments have been conducted. This study highlights the vulnerability to overfishing of these resources and the urgent need to develop management strategies.     

Toward a Carbon Dioxide Neutral Industrial Park

The industrial park of Herdersbrug (Brugge, Flanders, Belgium) comprises 92 small and medium‐sized enterprises, a waste‐to‐energy incinerator, and a power plant (not included in the study) on its site. To study the carbon dioxide (CO₂) neutrality of the park, we made a park‐wide inventory for 2007 of the CO₂ emissions due to energy consumption (electricity and fossil fuel) and waste incineration, as well as an inventory of the existing renewable electricity and heat generation. The definition of CO₂ neutrality in Flanders only considers CO₂ released as a consequence of consumption or generation of electricity, not the CO₂ emitted when fossil fuel is consumed for heat generation. To further decrease or avoid CO₂ emissions, we project and evaluate measures to increase renewable energy generation. The 21 kilotons (kt) of CO₂ emitted due to electricity consumption are more than compensated by the 25 kt of CO₂ avoided by generation of renewable electricity. Herdersbrug Industrial Park is thus CO₂ neutral, according to the definition of the Flemish government. Only a small fraction (6.6%) of the CO₂ emitted as a consequence of fossil fuel consumption (heat generation) and waste incineration is compensated by existing and projected measures for renewable heat generation. Of the total CO₂ emission (149 kt) due to energy consumption (electricity + heat generation) and waste incineration on the Herdersbrug Industrial Park in 2007, 70.5% is compensated by existing and projected renewable energy generated in the park. Forty‐seven percent of the yearly avoided CO₂ corresponds to renewable energy generated from waste incineration and biomass fermentation.     

A divergent lineage among Octopus minor (Sasaki, 1920) populations in the Northwest Pacific supported by DNA barcoding

Octopus minor (Sasaki, 1920) is an important economic fishery resource in China. In order to explore the stock information and the phylogeographic status of O. minor, mitochondrial DNA cytochrome c oxidase subunit I (COI, 565?bp) and 16S rRNA (493?bp) genes were amplified from 11 different sampling locations. Genetic diversity evaluated by haplotypic and nucleotidic diversity implied high diversity in Lianjiang, and relatively low diversity in Rongcheng, which suggests that effective measures to protect the O. minor resource in this area are urgently required. Private haplotypes and remarkable higher pairwise Φ_ST in Yilan are responsible for the deep genetic divergence between Yilan and the 10 other populations. Haplotypes networks and two clusters’ topological structure also support the distinct subgroups (lineages A and lineages B), which apparently possess smaller genetic variation than mean interspecies distance. Taiwan island and its strait may act as a natural barrier that restricts the gene flow from the mainland. Deep genetic divergence between mainland and Taiwanese east coasts suggests different genetic stock, indicating that different management strategies are required.