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1.
The blue mussel Mytilus trossulus occurs in the Pacific and in the North Atlantic. We developed and characterized six microsatellite loci for Baltic M. trossulus. Seventeen microsatellite loci were screened, of which six were polymorphic. The number of alleles among 50 individuals ranged
from 3 to 13 and the observed and expected heterozygosity were 0.09–0.46 and 0.34–0.86, respectively. The loci were also tested
for cross amplification in M. edulis, in which four of the six microsatellite loci were successfully amplified. 相似文献
2.
María Calero-Riestra Fernando Alda Jose Antonio Dávila Jesús T. García 《European Journal of Wildlife Research》2012,58(1):379-383
New microsatellite loci for Tawny Pipit Anthus campestris were isolated from a genomic library. We were able to unambiguously score six loci: two were dinucleotide, one trinucleotide,
two tetranucleotide and one pentanucleotide that turned out to be sex-linked. Four out of six loci were polymorphic with 7–23
alleles in our population and an observed heterozygosity ranging between 0.286 and 0.936. Cross-utility of these markers was
tested in other 17 steppe-bird species of six families. In addition, 16 microsatellite loci developed for other species were
tested for cross-species amplification in A. campestris. Eight microsatellite markers were successfully amplified; seven of them were polymorphic with 2–43 alleles and an observed
heterozygosity of 0.040–0.863. Overall, 14 functional locus markers have been characterized for A. campestris that could be useful for future studies of paternity, genetic variability and population structure. 相似文献
3.
Woo-Jin Kim Young-Ok Kim Bo-Hye Nam Hee Jeong Kong Eun-Mi Park Jung-Ha Kang Jeong-Ho Lee Kyung-Kil Kim 《Conservation Genetics》2009,10(4):1105-1111
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 81 polymorphic microsatellite
markers from 4,940 ESTs of the olive flounder, Paralichthys olivaceus. Out of 100 EST-derived microsatellites for which PCR primers were designed, 81 loci were polymorphic in 30 individuals from
a single natural population with 2–28 (mean 10.6) alleles per locus. The observed and expected heterozygosities of these loci
were 0.033–1.000 and 0.033–0.965, respectively. Segregation analysis within a mapping family revealed non-amplifying null
alleles at five loci. These new EST-derived microsatellite markers should be useful for population genetic analyses, pedigree
tracing and constructing a linkage map for olive flounder. 相似文献
4.
Eleven novel polymorphic microsatellite loci developed from a microsatellite enriched genomic library, are presented for the
Australian tree frog Litoria peronii. We screened 29 individuals from a single population and detected high levels of polymorphism for all 11 loci with the number
of alleles/locus ranging from 9 to 24. Values of expected and observed heterozygosities ranged from 0.789–0.955 and 0.207–1.00,
respectively. These microsatellite markers should prove useful in determining levels of genetic diversity, measuring gene
flow and migration, assigning individuals to their most likely population of origin, and in the assignment of paternity. 相似文献
5.
Marta Dubreuil Federico Sebastiani Maria Mayol Santiago C. González-Martínez Miquel Riba Giovanni G. Vendramin 《Conservation Genetics》2008,9(6):1665-1668
Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight
primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals
from 5 different populations (10–26 samples per population) were used to estimate genetic diversity parameters. High levels
of genetic diversity, with values ranging from 0.533 to 0.929 (6–28 alleles per SSR) were found. No linkage disequilibrium
between pairs of loci was detected. All loci but one showed significant departures from Hardy–Weinberg equilibrium. Excess
of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size
and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical
scales and to monitor gene flow. 相似文献
6.
Cristaria plicata was an important freshwater mussel for pearl culture in China. 18 polymorphic microsatellite markers were isolated and characterized
using (CA)15-enriched genomic library of C. plicata. These loci showed high levels of genetic polymorphism testing on 60 individuals sampled from Poyang Lake of Jiangxi Province,
China. The number of alleles per locus ranged from 4 to 18. The expected (H
E) and observed heterozygosities (H
O) were 0.7232–0.8961 and 0.0000–1.0000, respectively. Four microsatellite loci were significantly deviated from Hardy–Weinberg
equilibrium and no linkage disequilibrium was found. These microsatellite loci will be useful for assessment of genetic diversity
and population structure in C. plicata. 相似文献
7.
Marta Vila Neus Marí-Mena Shen-Horn Yen Carlos Lopez-Vaamonde 《Conservation Genetics》2010,11(3):1151-1154
Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals
from one population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from
5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was
0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium
at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from
this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this
highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna. 相似文献
8.
Robert Andrew King Sarah L. Harris Angela Karp Jacqueline H. A. Barker 《Tree Genetics & Genomes》2010,6(2):247-258
We present nine polymorphic di- and tri-nucleotide repeat nuclear microsatellite markers selected specifically for their use
in high throughput studies concerning the dioecious allotetraploid Salix alba–Salix fragilis willow complex. These taxa and their hybrids are difficult to discriminate using morphological characters. Thus, multiplex
reactions were developed for these microsatellite loci and their effectiveness to distinguish individuals, especially hybrids,
and their inheritance patterns in controlled crosses were determined. All loci displayed disomic–monogenic inheritance which
allowed for the genotype data to be analysed as for a diploid organism. The nine loci produced a total of 67 alleles (mean,
7.4 alleles per locus; range, 3–11 alleles) in a reference panel of 57 individuals from two germplasm collections and natural
populations. Gene diversity values (as measured by the expected heterozygosity) ranged from 0.000–0.820. A total of 53 distinct
multilocus genotypes were observed, and ordination analysis revealed three separate clusters corresponding to S. alba, S. fragilis and hybrids. The microsatellite loci described here will be used in population genetic studies to investigate genetic variation,
gene flow, levels of hybridisation and the extent of introgression in natural populations of the S. alba–S. fragilis complex. They are also useful for clonal identification, conservation and sustainable management of germplasm collections,
genetic mapping and the selection of individuals and/or certification of controlled crosses for breeding programmes. 相似文献
9.
Chi-Hang Chan Yudi Zhao Mei-Yan Cheung Geoffrey K. Chambers 《Conservation Genetics》2008,9(4):1067-1070
The kakerori (Pomarea dimidiata) is an endangered forest bird in the Cook Islands, South Pacific. We have developed 10 microsatellite markers using kakerori
feathers as the DNA source. Seven of these loci were found to be polymorphic in 42 individuals examined. The number of alleles
per locus in the polymorphic loci varied from 3 to 5. Observed and expected heterozygosity ranges were 0.57–0.74 and 0.50–0.74,
respectively. All loci isolated conformed to Hardy–Weinberg equilibrium expectations. We believe these loci will be useful
in studying kakerori conservation genetics, and our success in developing microsatellite markers from feather samples will
encourage the use of less invasive sample sources in microsatellite isolation studies. 相似文献
10.
We describe the isolation and characterization of ten microsatellite loci from the red-winged tinamou (Rhynchotus rufescens) and also evaluated the cross-amplification of these loci and other ten loci previously developed for the great tinamou (Tinamus major) in other tinamous. Genetic variability was assessed using 24 individuals. Six loci were polymorphic with moderate to high
number of alleles per locus (2–12 alleles) and showed expected heterozygosity (HE) ranging from 0.267 to 0.860. All loci conformed
to the Hardy–Weinberg expectation and linkage disequilibrium was not significant for any pair of loci. This battery of polymorphic
loci showed high paternity exclusion probability (0.986) and low genetic identity probability (4.95 × 10−5), proving to be helpful for parentage tests and population analyses in the red-winged tinamou. The cross-amplification was
moderate where of the 160 locus/taxon combinations, 46 (28.75%) successfully amplified. 相似文献
11.
In order to study hybridisation, taxonomic boundaries and phylogeography in the genus Pseudopanax (Araliaceae), we developed seven novel microsatellite loci from enriched genomic libraries of P. lessonii and P. crassifolius. These loci were characterised in 16 individuals from a single population of P. crassifolius, and displayed 2–11 alleles per locus. Observed heterozygosities ranged from 0.063 to 0.688. Most loci were polymorphic in
the closely related Pseudopanax species, and several loci amplified widely across the Araliaceae. 相似文献
12.
Isolation and characterization of microsatellite markers for the endangered <Emphasis Type="Italic">Taxus yunnanensis</Emphasis> 总被引:1,自引:0,他引:1
Ying-Chun Miao Jian-Rong Su Zhi-Jun Zhang Hui Li Jing Luo Ya-Ping Zhang 《Conservation Genetics》2008,9(6):1683-1685
Eleven polymorphic microsatellite loci were characterized for the endangered conifer Taxus yunnanensis. Eight loci were isolated through SSR-anchored PCR, one locus was developed by cross-species amplification tests, while the
last two loci were obtained from cross-species microsatellite sequences available in GenBank. Variability of these markers
was tested in 48 individuals collected from its main distribution range in Southwest China. The number of alleles per locus
ranged from 2 to 9, and the observed and expected heterozygosity varied from 0.000 to 0.625 and from 0.062 to 0.853, respectively.
Ten of these eleven loci were significantly deviated from Hardy–Weinberg expectation, except TS07 which showed a distinct
heterozygote excess. The availability of these new polymorphic microsatellite markers will provide an ideal marker system
for detailed population genetics studies in T. yunnanensis and potentially also for closely related species. 相似文献
13.
Soo Hyung Eo Woo-Shin Lee Travis C. Glenn Kenneth L. Jones Eun-Jae Lee Yong-Su Park John P. Carroll Shin-Jae Rhim 《Conservation Genetics》2009,10(6):1923-1925
We isolated and characterized eight polymorphic microsatellite DNA markers from the Korean field mouse, Apodemus peninsulae. The primers developed in this study yielded an average polymorphic information content of 0.78 (range 0.44–0.90), with an
average of 10.9 alleles per locus (range 5–16). Observed and expected heterozygosities ranged from 0.46 to 1.00 and from 0.49
to 0.93, respectively. These polymorphic loci may provide useful tools for understanding the species’ genetic structure and
ecology. 相似文献
14.
Achnatherum sibiricum is a threaten and toxic perennial bunchgrass mainly in the north of China. We developed 11 polymorphic microsatellite loci
from A. sibiricum by combining biotin capture method. After validating and scoring, these loci were polymorphic in a test population with the
range of alleles from four to 13 per locus. The observed and expected heterozygosities ranged from 0.1649 to 0.5624 and from
0.3071 to 0.8826, respectively. All 11 microsatellite markers were in Hardy–Weinberg equilibrium. These polymorphic microsatellites
will be useful for genetic diversity analysis and population structure construction for A. sibiricum. 相似文献
15.
In order to investigate the genetic diversity and structure of Hieracium pilosella L., we developed eleven pairs of primers for nuclear microsatellites through screening of three genomic DNA enriched libraries.
About 127 individuals from six locations of the Trentino region (Italy) were analysed. These loci were all polymorphic and
displayed 5–33 alleles per locus. These microsatellite markers constitute an efficient tool in investigating the genetic patterns
of H. pilosella L. populations. 相似文献
16.
Kahraman Gürcan Shawn A. Mehlenbacher 《Molecular breeding : new strategies in plant improvement》2010,26(3):551-559
Polymorphic microsatellite markers were developed for European hazelnut (Corylus
avellana L.) from the sequences of inter-simple sequence repeat (ISSR) fragments and flanking regions. Twenty-five ISSR primers were
used to generate fragments for cloning. Of the 520 unique sequences obtained, 41 contained long internal repeats (≥20 bp)
with flanking sequences sufficient for primer design. From these, we developed 23 new polymorphic microsatellite loci. The
flanking sequences were obtained for fragment ends by chromosome walking, and an additional 47 polymorphic markers were developed.
Two additional polymorphic markers were developed from a GA-enriched library. The 72 new marker loci were characterized using
50 diverse hazelnut accessions. For the internal repeat loci, the number of alleles per locus ranged from 2 to 16, with a
mean of 7.52. Mean values for expected heterozygosity (He), observed heterozygosity (Ho), and polymorphism information content (PIC) were 0.62, 0.59, and 0.58, respectively. The estimated frequency of null alleles
(r) was ≥0.05 at six of the 23 loci. For the 47 marker loci developed from fragment ends, the number of alleles per locus ranged
from 2 to 16, with a mean of 7.30. Mean values for He, Ho, and PIC were 0.62, 0.47, and 0.58, respectively. The estimated frequency of null alleles (r) was ≥0.10 at 18 of the 47 loci. Of the 70 loci developed from ISSR and flanking sequences, 50 segregated in our mapping
population and were assigned to linkage groups. 相似文献
17.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
18.
Song-Lin Chen Shi-Chao Xing Gen-Bo Xu Xiaolin Liao Jing-Feng Yang 《Conservation Genetics》2009,10(5):1469-1471
Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library
of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis. 相似文献
19.
Selvaraju Kanagarajan Saraladevi Muthusamy Emily Chin-Fun Chen Hsin-Sheng Tsay 《Conservation Genetics》2009,10(5):1487-1490
We developed and characterized 8 polymorphic microsatellite loci or simple sequence repeats from the expressed sequence tags
database of the medicinal fungus, Antrodia cinnamomea. Variability was assessed in a panel of 23 strains collected from various regions of Taiwan. The number of alleles per polymorphic
locus ranged from two to seven loci with an average of 4.375 alleles per locus. The observed and expected heterozygosity values
ranged from 0.130 to 0.783 and from 0.122 to 0.809, respectively. No significant Hardy–Weinberg equilibrium was detected (P < 0.01) at all loci. The EST-SSR markers developed in the present study can be used for strain identification and population
genetic studies in A. cinnamomea. 相似文献
20.
Isolation and characterization of polymorphic microsatellite loci from fat greenling (Hexagrammos otakii) 总被引:2,自引:0,他引:2
Song-Lin Chen Gui-Dong Miao Chang-Wei Shao Yong-Sheng Tian Xiaolin Liao 《Conservation Genetics》2009,10(5):1429-1431
Fat greenling (Hexagrammos otakii) is an economocally important marine fish species. Thirtyfive microsatellite loci were isolated from two enriched genomic
library of Hexagrammos otakii. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.2581 to 1.0000 and from 0.2892 to 0.7726, respectively. Four loci significantly
deviated from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between
pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Hexagrammos otakii. 相似文献