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短梗霉多糖发酵条件的研究 总被引:1,自引:1,他引:0
在摇瓶发酵条件研究的基础上。于16L自控发酵罐上进行了罐上发酵条件优化研究。发现以10%淀粉水解物为碳源时,淀粉水解物的最适DE值为40-50,发酵培养基中的硫酸铵最适用量不同于摇瓶发酵时的量,种龄和接种量、通气量、罐压、搅拌速度和搅拌叶轮挡数等均对多糖的产生有较大的影响。另外还进行了发酵过程的动力学的研究。 相似文献
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对一株野生型的出芽短梗霉(Aureobasidiumpullulans)Ftl和从Ftl出发经原生质体再生筛选出的菌株R45进行了摇瓶发酵产普罗蓝糖的比较研究,结果表明R45无论从形态,菌体生长情况,还是从普罗蓝糖的产量,黑色素的产生等方面都与亲株Ftl有明显的区别,说明R45是一株具有一定生产价值的变异菌株。 相似文献
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^60Coγ射线对不同细胞形态出芽短梗霉的辐射诱变效应 总被引:2,自引:0,他引:2
使用^60Coγ射线辐照诱变的方法处理出芽短梗霉AureobasidiumpullulansAP92菌株的原生质体,菌丝体片段,分生孢子悬液,经初筛,复筛与对突变株的遗传稳定性研究,发现采用原生质体进行诱变,所获突变株的正突变率,单株产多糖的提高幅度,正突变株的产多糖遗传稳定性均明显高于菌丝体与分生孢子,比较出发菌株AP92与经原生质体诱变获得的正突变株A81的性能,有如下明显改善,产多糖能力从1 相似文献
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Abstract Production of pullulan by five strains of Aureobasidium pullulans was compared in three media with three carbohydrate sources. Our goal was to screen strains and media to obtain pullulan in maximal yield, purity, and stability. Pullulan yields and properties were strongly affected by strain specificity, but a single medium performed best with most strains. Sucrose was the preferred carbohydrate for all five strains. A "color variant" strain of Aureobasidium , NRRL Y-12974, possibly representing a distinct species, produced a polysaccharide of intermediate molecular weight which was stable during storage at 4°C, heating to 100°C, and high shearing action. This polysaccharide was 70% pullulanase sensitive and contained the least contaminating melanin pigment. 相似文献
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普鲁兰多糖应用现状研究 总被引:2,自引:0,他引:2
YANG Xueling 《武汉生物工程学院学报》2007,(3)
介绍了普鲁兰多糖的理化性质和生理功能,阐述了其在农产品、食品、环保、包装、医药、石油等领域的应用现状,并对其良好的市场前景进行了展望。 相似文献
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K. Takizawa K. Fukushima Y. Maebayashi K. Okada K. Nishimura M. Miyaji 《FEMS microbiology letters》1992,92(2):120-132
Abstract A naturally occurring member of ubiquinone (Q) group, a dihydroubiquinone-9 (Q-9 (H2 )), has been isolated as a minor ubiquinone component from the fungus Aureobasidium pullulans . By ultraviolet absorption, mass and nuclear magnetic resonance spectrometric studies, the structure of Q-9 (H2 ) was found to be 2,3-dimethoxy-5-methyl-6-IX-dihydromultiprenyl9 -1,4-benzoquinone (I). 相似文献
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Protoplasts prepared from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans can take up heavy metals such as Zn2+, Co2+, Cd2+ and Cu2+. In relation to intact cells, the sensitivity of protoplasts to Cu2+ and Cd2+ was increased although chlamydospore protoplasts were more tolerant than yeast-like cell protoplasts. Surface binding of metals was reduced in protoplasts as compared with intact cells and this reduction was particularly evident for chlamydospore protoplasts. At the highest concentrations used, uptake of Zn2+, Co2+ and Cd2+ by yeast-like cell protoplasts was greater than that observed in intact cells which may have been due to toxicity, especially for Cd2+, resulting in increased membrane permeability, though for Zn2+ and Co2+ some barrier effect of the cell wall could not be completely discounted. Chlamydospore protoplasts were capable of intracellular metal uptake, unlike intact chlamydospores, and for Zn2+, uptake appeared to be via a different system less specific than that of the other cell types. For chlamydospores, the use of protoplasts confirmed the importance of the cell wall in preventing entry of metal ions into the cell. 相似文献
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Abstract Two mutants of the fungus Aureobasidium pullulans ATCC 42023 were isolated that exhibited elevated polysaccharide production. Both mutants were isolated using a combination of chemical mutagenesis and resistance to growth inhibitors. It was found that both mutants elaborated higher polysaccharide levels after 7 days of growth on corn syrup or sucrose, respectively, compared to ATCC 42023. The dry weights of the mutant cells were found not to differ greatly from those of the parent cells whether corn syrup or sucrose served as the carbon source. The pullulan content of the polysaccharide synthesized by the mutants or parent cells on sucrose was consistently lower than polysaccharide synthesized on corn syrup. Using corn syrup as a carbon source, the pullulan content of the polysaccharide elaborated by the parent was higher than either mutant. The inverse was found to occur with respect to pullulan content when the strains were grown on sucrose as a carbon source. 相似文献
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茁芽短梗霉原生质体激光诱变及高产菌株筛选 总被引:2,自引:0,他引:2
目的:通过普鲁兰(pullulan)产生菌-茁芽短梗霉(Aureobasidium Pullulans)原生质体的激光诱变,以得到普鲁兰高产菌株。方法:利用正交实验研究了茁芽短梗霉原生质体的制备与再生并确定了其最佳条件为:菌体以1%的甘氨酸预处理;在0.1mol/L pH 6.0柠檬酸一柠檬酸钠缓冲液,含0.7mol/L NaCl的高渗稳定液中;经蜗牛酶0.2%、纤维素酶0.1%、溶菌酶0.2%的混合酶酶解15min。采用He-Ne激光诱变茁芽短梗霉原生质体筛选得到普鲁兰高产菌株J208,其蔗糖转化率达到53.3%,是原始菌株的10.6倍。结论:用激光诱变茁芽短梗霉原生质体是获得普鲁兰高产菌株的新途径。 相似文献
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A stress-induced "mycosome" phase of Aureobasidium pullulans consisting of minute reproductive propagules that may revert directly to walled yeast cells is described. Mycosomes detected
by light- and electron-microscopy reproduce within senescent plant plastids, and display three developmental pathways: wall-less
cells (protoplasts), yeast cells, or membrane-bounded spherules that harbor plastids. Widespread in plant and algal cells,
mycosomes are produced by both ascomycete and basidiomycete fungi.
Electronic Publication 相似文献
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运用离子交换层析法和凝胶过滤层析法分离纯化普鲁兰多糖高产菌株Y68胞内葡萄糖基转移酶(简称GTF),并以SDS-PAGE、Native-PAGE对蛋白质进行量化、比较及特性鉴定,研究其酶学特性。结果表明短梗霉Y68中GTF被分离纯化,纯化酶在SDS-PAGE凝胶电泳上显示分子量50.8 ku单一条带,而在Native-PAGE凝胶电泳上显示分子量350 ku单一条带。纯化酶的最适酶反应pH和最适酶反应温度分别为6.0和40℃,酶对pH十分敏感,稳定性较差,对温度的稳定性则稍好。GTF为金属酶,Na+和K+对酶有激活作用,Ca2+、Mn2+、Mg2+、Ba2+、Cu2+、Fe2+、Hg2+、Co2+对酶活性有抑制作用,Hg2+对酶活性的抑制作用说明酶活性中心含有二锍键。EDTA、PMSF、SDS和碘乙酸对GTF活性有很大的抑制作用,而二硫苏糖醇(DTT)对酶活性有保护作用。 相似文献