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1.
Differences in the number of chromosomes with secondary constrictions and in the size of the C-band region on certain chromosomes have been observed among the following inbred strains of Mus musculus: C57BL/10J, C57BR/cdJ, DBA/1J, CBA/J, BALB/cJ, and AKR. These differences are useful as indicators of the location of rRNA genes and as normal chromosome markers. The size of each C-band region appears to remain constant over many generations. Only one probable change in the size of a C-band region was found. 相似文献
2.
V. G. Dev D. A. Miller R. Tantravahi R. R. Schreck T. H. Roderick B. F. Erlanger O. J. Miller 《Chromosoma》1975,53(4):335-344
Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M. m. musculus (the laboratory mouse) and M. m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences were observed in the centromeric region of some chromosomes. The two subspecies had very different distributions of C-band material. Antibodies to 5-methylcytosine were bound to regions of the chromosome corresponding to the C-bands in each animal. These findings support the idea that satellite DNA, which is concentrated in the C-band region, changes more quickly than bulk DNA. The interfertility of these two subspecies permits the development of a musculus strain carrying normal marker chromosomes for genetic studies. 相似文献
3.
The ZW sex chromosomes of the European eel, Anguilla anguilla, and the American eel, A. rostrata, were examined with C-band and fluorescent staining to demonstrate the C-band heterochromatin. The W as well as Z chromosomes in both species are C-band negative except for a small amount of C-band heterochromatin in the centromeric region, in contrast to the W or Y elements of most other vertebrates. No fluorescing W-associated body is evident either in interphase nuclei or in metaphase plates. The ZW chromosomes of the two species have substantially similar size, morphology, and patterns of C-band heterochromatin. Karyologic and evolutionary implications are discussed. 相似文献
4.
Jana Albrechtová Tomá? Albrecht Ludovít ?ureje Vincent A. Pallazola Jaroslav Piálek 《PloS one》2014,9(12)
Being subject to intense post-copulatory selection, sperm size is a principal determining component of male fitness. Although previous studies have presented comparative sperm size data at higher taxonomic levels, information on the evolution of sperm size within species is generally lacking. Here, we studied two house mouse subspecies, Mus musculus musculus and Mus musculus domesticus, which undergo incipient speciation. We measured four sperm dimensions from cauda epididymis smears of 28 wild-caught mice of both subspecies. As inbred mouse strains are frequently used as proxies for exploring evolutionary processes, we further studied four wild-derived inbred strains from each subspecies. The subspecies differed significantly in terms of sperm head length and midpiece length, and these differences were consistent for wild mice and wild-derived strains pooled over genomes. When the inbred strains were analyzed individually, however, their strain-specific values were in some cases significantly shifted from subspecies-specific values derived from wild mice. We conclude that: (1) the size of sperm components differ in the two house mouse subspecies studied, and that (2) wild-derived strains reflect this natural polymorphism, serving as a potential tool to identify the genetic variation driving these evolutionary processes. Nevertheless, we suggest that more strains should be used in future experiments to account for natural variation and to avoid confounding results due to reduced variability and/or founder effect in the individual strains. 相似文献
5.
Summary Using the method of linear measurement, the lengths of constitutive heterochromatin of chromosomes 1, 9, 16, and Y were determined in 125 unrelated individuals, and in 30 members of ten families. The method used eliminates the variations in the C-band length due to different degrees of contraction of chromosomes in different mitoses, and enables the size of heterochromatin blocks to be expressed. It was found that the distribution of C-band lengths in the group of 125 individuals was normal, i.e., Gaussian, for all four classes of chromosomes measured. On the basis of length distribution and by computing the P1, P10, P90 and P99 percentiles, the actual numerical limits could be proposed for the five-step evaluation of heterochromatin length according to the Paris Conference (1971), Supplement (1975), for chromosomes 1, 9, 16, and in a preliminary way also for Y. When applying the proposed limits to data obtained in the present study, 165 C-band variants could be identified among the 125 individuals.In ten families, C-block lengths of the chromosomes transmitted from parents to progeny could be determined in 63 cases. The mean difference in C-band length of transmitted chromosomes, as measured in parents and in children, was 0.46×10-7 m. An analysis was carried out to detect the factors upon which the magnitude of this difference depends, and to define what differences are attributable to methodological errors. The results revealed that the difference rises slightly with the increasing length of the measured C block. Three degrees, defined by concrete ranges of difference in C-block length, were proposed for expressing the probability that the compared chromosomes had been transmitted.The study further attests to the effectiveness of the method of constitutive heterochromatin measurement for paternity testing. In our set of ten families, the comparison of C-band lengths of chromosomes 1, 9, 16, and Y led to rejection of paternity in 64% of unrelated individuals; excluding the Y chromosome, the percentage decreased to 61. As many as 47% of the individuals were rejected by a difference higher than two units (i.e., transmission of the compared chromosome highly improbable). 相似文献
6.
The segregation pattern of chromosomes 14 and 18 were analyzed in recombinant inbred strains of mice developed from KE and CBA/Kw strains. The analysis was possible owing to the fact that the C-band on chromosomes 14 of the CBA/Kw strain and that of chromosome 18 of the KE strain show size polymorphism: while the CBA/Kw mice have a small sized C-band on chromosome 14, the KE mice show small C-bands on chromosome 18. Chromosomes were identified by G-banding and FISH. The results show that the chromosomes with small centromeric chromatin segregate preferentially. 相似文献
7.
Stanley K. Sessions 《Chromosoma》1980,77(2):157-168
The mitotic chromosomes of the neotenic (sensu Gould, 1977, and Alberch et al., 1979) salamander Necturus maculosus (Rafinesque) have been examined using a C-band technique to demonstrate the distribution of heterochromatin. The C-banded mitotic chromosomes provide evidence of a highly differentiated XY male/XX female sex chromosome heteromorphism, in which the X and Y chromosomes differ greatly in size and morphology, and in the amount and distribution of C-band heterochromatin. The X chromosome represents one of the largest biarmed chromosomes in the karyotype and is indistinguishable from similar sized autosomes on the basis of C-band heterochromatin. The Y chromosome, on the other hand, is diminutive, morphologically distinct from all other chromosomes of the karyotype, and is composed almost entirely of C-band heterochromatin. The discovery of an X/Y chromosome heteromorphism in this species is consistent with the observation by King (1912) of a heteromorphic spermatogenic bivalent. Karyological and phylogenetic implications are discussed. 相似文献
8.
Nucleolus Organizers in MUS MUSCULUS Subspecies and in the Rag Mouse Cell Line 总被引:1,自引:0,他引:1
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Silver staining has been used to detect active nucleolus organizer regions (NOR's). By this criterion six mouse chromosomes, numbers 12, 15, 16, 17, 18 and 19, can have an NOR. The number and distribution of chromosomes with NOR's vary among inbred strains of Mus musculus musculus (C57BL/6J, BALB/cJ, C3H/HeJ and C3H/StCr1BR) and in M. musculus molossinus. In a musculus x molossinus F1 hybrid, nucleolus organizers from each parent are silver stained.—Chromosomes which have NOR's in diploid cells also show them in tetraploid cells and in established cell lines. The BALB/cJ strain shows Ag-staining of NOR's on chromosomes 12, 15, 18 and occasionally 16. In the RAG cell line, which was derived from BALB/c, active NOR's are seen on 12, 15 and 18, even after these chromosomes have undergone structural rearrangements in the cell line. Some correlation exists between the amount of Ag-stain and the size of a secondary construction region, with a large amount of Ag-stain present on a chromosome which has a prominent secondary constriction. There is no correlation between the amount of Ag-stain and the presence or absence of C-band material. 相似文献
9.
Mouse chromosomes, with the exception of the Y chromosome, are telocentric. The telomere at the p-arm is separated from the centromere by the tL1 sequence and TLC tandem repeats. A previous report showed that the TLC array was also conserved in other strains of the subgenus Mus. These results suggest that the TLC arrays promote the stable evolutionary maintenance of a telocentric karyotype in the subgenus Mus. In this study, we investigated the degree of conservation of TLC arrays among a variety of wild-derived inbred strains, all of which are descendants of wild mice captured in several areas of the world. Genomic PCR analysis indicates that the sequential order of telomere-tL1 is highly conserved in all strains, whereas tL1-TLC is not. Next, Southern blot analysis of DNAs isolated from a panel of mouse subspecies showed both Mus musculus domesticus and Mus musculus castaneus subspecies possess TLC arrays. Unexpectedly, this repeat appears to be lost in almost all Mus musculus musculus and Mus musculus molossinus subspecies, which show a clear geographic divide. These results indicate that either other unknown sequences were replaced by the TLC repeat or almost all M. m. musculus and M. m. molossinus subspecies do not have any sequence between the telomere and minor satellites. Our observation suggests that the TLC array might be evolutionarily unstable and not essential for murine chromosomal conformation. This is the first example of the subspecies-specific large genome alterations in mice. 相似文献
10.
Liu YH Takahashi A Kitano T Koide T Shiroishi T Moriwaki K Saitou N 《Genes & genetic systems》2008,83(1):77-88
Patterns of genetic variation provide insight into the evolutionary history of a species. Mouse (Mus musculus) is a good model for this purpose. Here we present the analysis of genealogies of the 21 nuclear loci and one mitochondrial DNA region in M. musculus based on our nucleotide sequences of nine inbred strains from three M. musculus subspecies (musculus, domesticus, and castaneus) and one M. spicilegus strain as an outgroup. The mitochondrial DNA gene genealogy of those strains confirmed the introgression pattern of one musculus strain. When all the nuclear DNA data were concatenated to produce a phylogenetic tree of nine strains, musculus and domesticus strains formed monophyletic clusters with each other, while the two castaneus strains were paraphyletic. When each DNA region was treated independently, the phylogenetic networks revealed an unnegligibly high level of subspecies admixture and the mosaic nature of their genome. Estimation of ancestral and derived population sizes and migration rates suggests the effects of ancestral polymorphism and gene flow on the pattern of genetic variation of the current subspecies. Gene genealogies of Fut4 and Dfy loci also suggested existence of the gene flow between M. musculus and M. spicilegus or other distant species. 相似文献
11.
We have searched for genetic variation in the expression of salivary androgen-binding protein (ABP) in a wide variety of mice and other rodents. ABP was present in the salivas of mice of all species and subspecies studied. Genetic studies have identified three common variants of the ABP Alpha subunit (Abpaa, Abpab, and Abpac) in Mus musculus populations with distributions that correspond roughly to those of the subspecies studied (domesticus, musculus, and castaneus, respectively). It appears that the ABP a and b polymorphisms conform to the hybrid zone between the domesticus and musculus subspecies characterized by others. Our studies suggest that the presence of Abpab in inbred strains may be due to a M. m. musculus contribution, perhaps via oriental fancy mice bred to European mice in the early lines leading to the common inbred strains. The relatively common occurrence of the ABP a type in other Mus species leads us to conclude that it is the ancestral type in mice. Further, the observation of what amounts to unique alleles in the three different subspecies indicates that microevolution of the protein has occurred. In a broader survey, ABP was also found in the salivas of Murid and Cricetid rodents generally. These findings suggest that ABP has an important functional role in rodent salivas. 相似文献
12.
Yoshikazu Totsuka Yasumitsu Nagao Takuro Horii Hiromichi Yonekawa Hiroshi Imai Hideo Hatta Yoshiaki Izaike Tomoyuki Tokunaga Yoriko Atomi 《Journal of applied physiology》2003,95(2):720-727
We compared four inbred mouse strains in their physical performance, measured as a maximal treadmill running time, characteristics of soleus muscle, anatomic character, and growth. The strains used were Mus musculus domesticus [C57BL/6 (B6) and BALB/c], Mus musculus molossinus (MSM/Ms), and Mus spretus. Maximal running time was significantly different among these four mouse strains. Running time until exhaustion was highest in MSM/Ms and lowest in M. spretus. Maximal times for the laboratory mouse strains were nearly identical. Soleus muscle fiber type and cross-sectional area also differed significantly among the species. In particular, M. spretus was significantly different from the other inbred mouse strains. Growth in the wild-derived inbred mice appeared to be complete earlier than in the laboratory mice, and the body size of the wild strains was about half that of the laboratory strains. From these results, we propose that wild-derived inbred mouse strains are useful models for enhancing phenotypic variation in physical performance and adaptability. 相似文献
13.
María Teresa Rodriguez-Gómez María José Martín-Sempere J. A. Abrisqueta 《Human genetics》1987,75(1):56-61
Summary The possible influence of total Y chromosome length and the C-band size variability of chromosomes 1, 9, 16, and Y, on reproductive wastage was investigated. One hundred couples with recurrent reproductive wastage and 106 control couples with at least two healthy children and no miscarriages were cytogenetically studied. Total Y chromosome length was evaluated as the Y/F index and the C-band size was analyzed quantitatively according to the linear measurement method of Baliek et al. (1977). The different degrees of mitotic contraction were corrected on the basis of the linear correlation found between heterochromatin and euchromatin length. Statistical comparison between results of Y chromosome from both samples demonstrated, in the test group, an increase in the mean value of the Y/F index, but the increase of Y C-band length did not reach significance. In addition mean values of C-band length on chromosomes 1, 9, and 16 in couples from the test group and especially those who had had two or more abortions, were lower than those in the controls. Among the latter the frequency of chromosomes included in the category of very large heterochromatin size is higher. However these length differences have been demonstrated only in specific subgroups, and in each one for a different chromosome. Our results indicated that Y chromosome length as well as C-band size variabilities are not directly related to reproductive wastage. 相似文献
14.
A. Lane Rayburn H. James Price J. D. Smith John R. Gold 《American journal of botany》1985,72(10):1610-1617
The number of mitotic chromosomal C-bands, the percent of the genome comprised of C-band heterochromatin, and genome size (4C DNA content) were determined for 22 North American inbred and open-pollinated lines of Zea mays. The number of C-bands ranged from 0 in Tama Knobless Flint to 18 in Zapolate Grande. The percent C-band heterochromatin ranged from 0% in Tama Knobless Flint to 16.9% in Tx601. Genome size varied over 23%: Gaspe Flint had the lowest DNA content (9.82 pg), and Zapolate Grande had the highest (12.12 pg). Genome size and the amount of heterochromatin were significantly correlated. The corn lines were assigned to five maturity zones encompassing a south-to-north range from Mexico to Canada. Significant negative correlations were detected between the amount of C-band heterochromatin and maturity zones, and between DNA content and maturity zones among the lines. It is speculated that the simultaneous selection by man for earlier maturation and plant size may be related to the lower DNA content of corn varieties adapted to higher latitudes. Such selection for larger plants may have been achieved through selection for more cells, which could result from the shorter mitotic cycle time that correlates with reduced DNA amount. 相似文献
15.
近交系小鼠遗传质量监测新方法的建立——染色体Q—H分带技术及其应用 总被引:3,自引:1,他引:2
本研究利用最新的Quinacrine Mustard and 33258 Hoechst(Q—H)复合荧光染色技术对10个品系的近交系小鼠的核型进行分析。在同一细胞内,按各号染色体着丝粒带大小排列、分组,建立该10个品系近交系小鼠特有的染色体着丝粒带核型,作为各品系小鼠遗传质量监测的染色体标记指标。本研究还对615小鼠品系的生化标汜检测与染色体标记检测的结果进行比较,同时比较了不同来源615小鼠的染色体标记,从而进一步阐明了该方法作为实验动物遗传监测方法之一与其他方法间的互补性及其自身特点。 相似文献
16.
Yan Yongshan Shun Ficin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(3):469-475
Summary The Chinese hedgehog has a diploid chromosome number of 48 in which there are eleven pairs of telo- or subtelocentric autosomes, twelve pairs of meta- or submetacentric autosomes, a metacentric X chromosome and a telocentric Y chromosome. The heterochromatin is almost completely distributed in five large distal segments of chromosomes nos. 9 to 12 and no. 18. There is no positive C-band in the centromeres of the chromosomes except for the X chromosome which has a small, weakly stained C-band in the centromere. In Chinese hedgehog cells 52.1% of SCEs are found at the junction between the euchromatin and the heterochromatin, 39.5% in the heterochromatin and 8.4% in the auchromatin. The SCE number per unit C-band is double the SCE number per unit euchromatin. The SCE rate in the heterochromatin or euchromatin regions is not proportional to their chromosome length and can be quite different between different pairs of the chromosomes. Our results indicate that there is a non-uniform distribution of the SCEs in the Chinese hedgehog cells. 相似文献
17.
In most species that reproduce sexually, successful gametogenesis requires recombination during meiosis. The number and placement of crossovers (COs) vary among individuals, with females and males often presenting the most striking contrasts. Despite the recognition that the sexes recombine at different rates (heterochiasmy), existing data fail to answer the question of whether patterns of genetic variation in recombination rate are similar in the two sexes. To fill this gap, we measured the genome-wide recombination rate in both sexes from a panel of wild-derived inbred strains from multiple subspecies of house mice (Mus musculus) and from a few additional species of Mus. To directly compare recombination rates in females and males from the same genetic backgrounds, we applied established methods based on immunolocalization of recombination proteins to inbred strains. Our results reveal discordant patterns of genetic variation in the two sexes. Whereas male genome-wide recombination rates vary substantially among strains, female recombination rates measured in the same strains are more static. The direction of heterochiasmy varies within two subspecies, Mus musculus molossinus and Mus musculus musculus. The direction of sex differences in the length of the synaptonemal complex and CO positions is consistent across strains and does not track sex differences in genome-wide recombination rate. In males, contrasts between strains with high recombination rate and strains with low recombination rate suggest more recombination is associated with stronger CO interference and more double-strand breaks. The sex-specific patterns of genetic variation we report underscore the importance of incorporating sex differences into recombination research. 相似文献
18.
Sabine Adolph 《Chromosoma》1988,96(2):102-106
In situ nick translation of mouse metaphase chromosomes by non-radioactive detection means and DNase I digestion followed by Giemsa staining were used to analyse the DNase I resistance of two different C-band positive regions. These were the centromeric heterochromatin of aero- and metacentric chromosomes and an interstitial C- band on chromosome 1 of wild mice, IS(HSR;1C5D)1Lub. Whereas the centromeric heterochromatin was clearly resistant to DNase I, the interstitial C-band showed very high DNase I sensitivity. Among centromeric C-bands, the heterochromatin in Robertsonian fusion biarmed chromosomes was more resistant to DNase I action than was the centromeric heterochromatin of the acrocentric chromosomes. 相似文献
19.
The mitotic chromosomes of several inbred strains of mice and a series of F(1) hybrids have been analyzed by quinacrine staining and further characterized by the centromeric heterochromatin banding (C-banding). Inbred strains had the same amount of C-banding material on homologous chromosomes but showed variation in the amount on different chromosomes. F(1) hybrids showed characteristics of each parent and it appears that the amount of C-banding on each chromosome is a simple inherited polymorphism. In this study 12 different chromosomes could be distinguished by their C-banding, and these can be used as normal chromosome markers. 相似文献
20.
Piálek J Vyskocilová M Bímová B Havelková D Piálková J Dufková P Bencová V Dureje L Albrecht T Hauffe HC Macholán M Munclinger P Storchová R Zajícová A Holán V Gregorová S Forejt J 《The Journal of heredity》2008,99(1):34-44
Two house mouse subspecies, Mus musculus domesticus and Mus musculus musculus, form a hybrid zone in Europe and represent a suitable model for inferring the genes contributing to isolation barriers between parental taxa. Despite long-term intensive studies of this hybrid zone, we still know relatively little about the causes and mechanisms maintaining the 2 taxa as separate subspecies; therefore, to gain insight into this process, we developed 8 wild-derived inbred house mouse strains. In order to produce strains as pure domesticus or musculus genomes as possible, the individuals used to establish the breeding colony for the 3 domesticus and 2 of the musculus strains were captured in the Czech Republic from wild populations at extreme western and eastern edges of the subspecific contact zone, respectively. The remaining 3 musculus strains were bred from mice captured about 250 km east of the hybrid zone. Genetic analysis based on 361 microsatellite loci showed that 82% of these markers are diagnostic for either the musculus or the domesticus strains. In order to demonstrate the potential utility of this genetic differentiation in such strains, phenotypic variation was scored for 2 strains from opposite edges of the hybrid zone and significant differences in morphology, reproductive performance, in vitro immune responses, mate choice based on urinary signals, and aggressiveness were found. In addition, the 3 strains derived from musculus populations far from the hybrid zone display significant differences in polymorphism in hybrid male sterility when crossed with the laboratory strains C57BL/6 or C57BL/10, which have a predominantly domesticus genome. Although further studies will be necessary to demonstrate intersubspecific differences, all analyses presented here indicate that these newly developed house mouse strains represent a powerful tool for elucidating the genetic basis of isolation barriers in hybrid zones and for studying speciation in general. 相似文献