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1.
We tested the hypothesis of negative fitness-density relationships and predicted that an increase in the density of parent fleas would result in lower survival rates and longer development time of pre-imagoes as well as shorter time to death from starvation of newly emerged imagoes. These predictions were experimentally tested on Xenopsylla conformis Wagner and Xenopsylla ramesis Rothschild feeding on two rodent species, Meriones crassus Sundevall or Dipodillus dasyurus Wagner. Survival of larvae and pupae, but not eggs, was negatively affected by parent density. An increase in parent density led to a decrease in the number of imagoes of the next generation. Eggs of both species developed faster when the parents were at low densities on either host. The same was true for larval X. ramesis, but not larval X. conformis. The negative effects of parent density on the duration of pupal development were evident in X. conformis, parents of which fed on both hosts, and X. ramesis from parents fed on M. crassus, whereas X. ramesis from parents fed on D. dasyurus developed faster at low densities. A negative effect of density on the development of offspring from egg to imago in X. conformis was manifested mainly when parent fleas fed on D. dasyurus, whereas the negative effect of density on offspring development in X. ramesis was manifested mainly when parent fleas fed on M. crassus. Although there was no general effect of parent density on the resistance of imago offspring to starvation, male X. ramesis from parents fed at the highest density survived starvation for significantly shorter times compared with those from parents fed at lower densities. Manifestation of the negative effect of parent density on offspring quality appears to vary with flea species and may be affected by host species.  相似文献   

2.
Abstract.  1. The fleas Xenopsylla conformis and Xenopsylla ramesis exploit the same rodent host, Meriones crassus , and replace each other between two different habitats situated at the opposite sides of a steep precipitation gradient. It was hypothesised that the reason for this paratopic distribution is competition between larvae of the two species for food resources.
2. This hypothesis was tested by studying the performance of larvae of the two species in terms of their developmental success in mixed-species and single-species treatments under different air temperatures, relative humidities, substrate textures, and food abundance.
3. The number of individuals of X. conformis that survived until emergence depended significantly on the presence of competing species, being, in general, lower in mixed-species compared with single-species treatments. The decrease in developmental success of X. conformis in mixed-species treatments was found mainly during food shortage. In contrast, presence of the competitor did not affect the number of X. ramesis that survived until emergence. No effect of the presence of the competitor on duration of development or sex ratio was found in either species.
4. The results of this study, together with the results of our previous studies, provide an explanation for the paratopic distribution of X. conformis and X. ramesis that exploit the same host species.  相似文献   

3.
Fleas Xenopsylla conformis mycerini and Xenopsylla ramesis replace each other on the same rodent host (Meriones crassus) in two habitats that differ in substrate texture (sand and loess-like sediments, respectively). We hypothesized that the substrate is an important factor determining flea distribution and studied survival of larvae, pupae and newly emerged adults as well as the rate of pre-imaginal development of these flea species in sand and loess rearing medium (= substrate). Texture of rearing medium did not affect survival and development rate of eggs in either X. c. mycerini or X. ramesis. Larval survival and the rate of development were both affected by the factor of substrate. Survival of X. c. mycerini larvae was significantly higher in sand than in loess substrate, whereas survival of X. ramesis larvae did not differ in different substrates. Larvae of both species developed faster in sand substrate than in loess substrate. Maximal survival time of X. c. mycerini larvae that died before pupation did not depend on substrate, whereas X. ramesis larvae survived significantly longer in loess than in sand substrate. Most pupae of both species survived successfully on both substrates, but the duration of pupal stages in sand substrate was longer than that in loess substrate in both species. Newly emerged adults of both species survived similar time in both sand and loess substrate. Irrespective of substrate, adult X. c. mycerini survived for a shorter time than did adult X. ramesis. No between-sex within-species differences in survival time of newly emerged adults in sand versus loess substrate were found in X. c. mycerini. Survival time of males and females of X. ramesis differed in sand substrate but not in loess substrate.  相似文献   

4.
Parasites should make the same decisions that every animal makes regarding fitness reward. They can maximize reproductive success by selection of those habitats that guarantee the greatest fitness output. We consider the host population as a habitat of a parasite population. Consequently, hosts (=habitats) that differ quantitatively or qualitatively will support different numbers of parasites. The nature of habitat selection can be detected by isodars, lines along which habitat selection yields equivalent fitness reward. We applied this approach to study host selection of five fleas, each infesting two desert rodents. Xenopsylla conformis, Xenopsylla ramesis, Nosopsyllus iranus theodori and Stenoponia tripectinata medialis parasitize Gerbillus dasyurus and Meriones crassus. Synosternus cleopatrae pyramidis parasitizes Gerbillus andersoni allenbyi and Gerbillus pyramidum. Three fleas ( X. conformis, X. ramesis and S. c. pyramidis) were able to perceive quantitative (amount of the resource; e.g. organic matter in the nest for flea larvae) and/or qualitative (pattern of resource acquisition; e.g. host defensiveness) differences between hosts. Two other fleas did not perceive between-host differences. X. conformis was a density-dependent host selector that showed sharp selectivity at low density. X. ramesis and S. c. pyramidis were density-independent host selectors with a direct correspondence of density with habitat quality. N. i. theodori and S. t. medialis were non-selectors with no relationship at all between density and host quality. The results of the application of the isodar theory suggest that ectoparasites, like other animals, behave as if they are able to make choices and decisions that favour environments in which their reproductive benefit is maximized.  相似文献   

5.
We hypothesized that sexual and interspecific differences in jumping performance of fleas found in our previous study are correlated with differences in resting metabolic rate (RMR) between sexes and among species. To test this hypothesis, we measured RMR of seven flea species (Xenopsylla conformis mycerini, Xenopsylla ramesis, Xenopsylla dipodilli, Parapulex chephrenis, Synosternus cleopatrae pyramidis, Nosopsyllus iranus theodori and Stenoponia tripectinata medialis). We compared RMR between sexes and among species and examined whether there is intra- and interspecific correlation between RMR and jumping ability. Both mass-specific and mass-independent RMR were the highest in female S. t. medialis, whereas mass-specific RMR was the lowest in male X. dipodilli and mass-independent RMR was the lowest in three Xenopsylla species and P. chephrenis. Mass-specific and mass-independent RMR were significantly higher in females than in males in all fleas except S. t. medialis. Differences in jumping ability between males and females were found to be correlated with sexual differences in mass-specific or mass-independent RMR. Interspecific comparison showed that the length of jump in both male and female fleas was strongly affected by their mass-specific and mass-independent RMR.  相似文献   

6.
Experiments were conducted on laboratory populations of Eriworm, Philosamia ricini Boisduval to study the effect of temperature and humidity on development, growth, survival and oviposition. Experiments were performed at four different temperatures (22 +/- 2 degrees C, 26 +/- 2 degrees C, 28 +/- 2 degrees C and 34 +/- 1 degrees C) each with three different humidities (50%, 70% and 90% relative humidity). Shortest developmental period (42 days) was observed in 28 +/- 2 degrees C and 70% RH. Longest developmental period was (63.6 days) observed in 22 +/- 2 degrees C at 50% RH. Highest larval weight (9.1 g) was found in 28 +/- 2 degrees C at 70% RH. Best pupal weight was observed in 26 +/- 2 degrees C at 70% RH. Weight of silk material was found to be maximum in 26 +/- 2 degrees C at 70% RH. Survival was best in 28 +/- 2 degrees C and 26 +/- 2 degrees C at 70% RH. Oviposition was found to be highest in 28 +/- 2 degrees C at 70% RH.  相似文献   

7.
1. 1. The mean durations of development in the pupae of Drosophila melanogaster (Meigen) and their survival were measured at combinations of six constant temperatures (15, 20, 22.5, 25, 27.5 and 30°C) and up to 11 levels of relative humidity. The thermal survival range for the pupae is between 15 and 30°C, and the humidity viable range is between 60 and 100% RH.
2. 2. The percentage water loss of the pupae was measured at six constant temperatures and four levels of relative humidity. There was a rapid increase in the percentage of water lost during the first 24 h exposure at all tested conditions. However, pupae reared at 100% RH at each constant temperature, sustained the lowest water loss. The percentage water loss increased as temperature increased, as humidity decreased and also with time.
3. 3. The duration of larval development studied at six constant temperatures (15, 20, 22.5, 25, 27.5 and 30°C) was inversely related to temperature. A wide range of alternating temperature regimes had a small, though statistically significant, accelerative effect on larval developmental time. Thus, the present results may be used as a basis for modelling development under changing temperatures, with the assumption that the developmental rate is nearly identical to that from a series of constant temperatures.
  相似文献   

8.
Xenopsylla ramesis is a flea species parasitizing gerbilline rodents in the deserts of the Middle East. This study was undertaken to determine metabolic requirements of the different developmental stages of the flea-life cycle as well as to investigate the metabolic response to temperature and starvation after blood feeding. A high resolution respirometry system was used to measure CO2 emission of fleas ranging in size from 0.166+/-0.006 mg (larvae) to 0.263+/-0.009 mg (adults). The free-living stages (larvae and adults) had significantly higher metabolic rates than the cocooned stages (pupae). CO2 emission rates of the larvae exceeded that of the adults by 2.6-fold and the pupae by 7.3 times. In the adults, both temperature and blood feeding significantly affected starvation-level metabolism. Metabolism was temperature dependent with an average Q10 of 2.57 for females and 2.55 for males over the temperature range of 10-30 degrees C. No consistent decline in thermal sensitivity at higher ambient temperatures was evident. Fleas that had a blood meal prior to starvation had significantly higher metabolic rates (0. 86 +/- 0.008 x 10(-3) ml mg(-1) h(-1)) than fleas, which were newly emerged unfed adults (0.56 +/- 0.1 x 10(-3) ml mg(-1) h(-1)). Water content also differed between fed (range approx. 67-69% body mass) and newly emerged adults (range approx. 73-75% of body mass). Feeding may stimulate some as yet undetermined physiological process that causes differential metabolic response in starving, fed and unfed fleas. Characteristics of gas exchange in desert-dwelling fleas are reflective of the off-host life style in the protected microenvironment of the host nest or burrow, rather than as a response to any type of environmental extreme.  相似文献   

9.
Data are given on the blood amount engorged by the fleas Xenopsylla conformis, X. cheopis, Leptopsylla segnis, Nosopsyllus laeviceps, N. consimilis, Ctenophthalmus golovi, Neopsylla setosa, Citellophilus tesquorum and Coptopsylla lamellifer. The average weight of blood portion in females of different species engorged for the first time ranged from 0.05 mg (X. conformis) to 0.72 mg (C. lamellifer). Females of most species, which had multiple blood meals, engorge more blood. Males engorge less blood than females and blood portions do not increase with age. In all sex-age groups most ectoparasites cease feeding spontaneously after having incomplete blood meal. Indices are suggested for the estimation of saturation rate in fleas during feeding.  相似文献   

10.
To counteract water loss due to excretion, cuticular transpiration and respiration, various groups of arthropods have developed mechanisms for active uptake of water vapor from unsaturated air. In this study, active uptake capabilities and water loss rates were examined in the various developmental stages of the cat flea, Ctenocephalides felis. To determine critical equilibrium humidity, the lowest relative humidity at which active water uptake can occur, pre-desiccated immature and adult fleas were placed in a series of humidity regimes ranging from 44 to 93% RH. Active uptake occurred in larval stages at relative humidities above 53% and in pre-pupae at 75-93% RH. Pupae and adults did not demonstrate active uptake at any humidity. Optimal uptake for larvae occurred between 20 and 30 degrees C. When placed over Drierite (<10% RH), larval and adult stages demonstrated a higher rate of water loss than pre-pupal and pupal stages. Active water uptake is necessary to ensure proper development of the larvae of C. felis. Active uptake ceases after the larval-pupal ecdysis and it appears that adults have lost the ability to actively uptake water.  相似文献   

11.
The factors favouring the cessation of reproduction in X. conformis are laid in the preimaginal state. The drop in temperature during the formation of imago at the pupal stage is a signal for the cessation of reproduction. Imagos hatched at a temperature lower than that of developmental conditions of preimaginal stages do not start reproduction and enter facultative imaginal diapause state. With further decrease in temperature the state of fleas intensifies. With the rise of temperature fleas come out of diapause. In autumn coming out of diapause begins at a temperature higher than 20 degrees, on the 8th--9th day. The lower air temperature the more rapid is coming out of diapause, at a rise of temperature of 3 to 5 degrees.  相似文献   

12.
Bartonella are emerging and re-emerging pathogens affecting humans and a wide variety of animals including rodents. Horizontal transmission of Bartonella species by different hematophagous vectors is well acknowledged but vertical transmission (from mother to offspring) is questionable and was never explored in fleas. The aim of this study was to investigate whether the rodent flea, Xenopsylla ramesis, can acquire native Bartonella from wild rodents and transmit it transovarially. For this aim, Bartonella-free laboratory-reared X. ramesis fleas were placed on six naturally Bartonella-infected rodents and six species-matched Bartonella-negative rodents (three Meriones crassus jirds, two Gerbillus nanus gerbils and one Gerbillus dasyurus gerbil) for 7 days, 12-14h per day. The fleas that were placed on the Bartonella-positive rodents acquired four different Bartonella genotypes. Eggs and larvae laid and developed, respectively, by fleas from both rodent groups were collected daily for 7 days and molecularly screened for Bartonella. All eggs and larvae from both groups were found to be negative for Bartonella DNA. Interestingly, two of five gut voids regurgitated by Bartonella-positive fleas contained Bartonella DNA. The naturally infected rodents remained persistently infected with Bartonella for at least 89 days suggesting their capability to serve as competent reservoirs for Bartonella species. The findings in this study indicate that X. ramesis fleas can acquire several Bartonella strains from wild rodents but cannot transmit Bartonella transovarially.  相似文献   

13.
Chomel BB 《Molecular ecology》2011,20(13):2660-2661
In this issue of Molecular Ecology, Morick et al. (2011) present an interesting study of acquisition and transmission of Bartonella by Xenopsylla ramesis fleas (Fig. 1) which infest naturally wild desert rodents from the Negev desert. A major issue with vector-borne diseases and vector-borne infection is to know whether the vector can also be a natural reservoir and transmit the infectious agent transovarially, allowing the infection to be perpetuated through successive generations of vectors. The desert flea, X. ramesis, is a flea species parasitizing gerbilline rodents in the deserts of the Middle East (Fielden et al. 2004).  相似文献   

14.
Bionomics of fleas (Siphonaptera) parasitizing rodent hosts, mostly the gerbil Gerbillus dasyurus (Wagner) and the jird Meriones crassus Sundevall (Gerbillidae), were investigated in the central Negev desert of Israel. Populations were sampled weekly (by Sherman trapping of hosts) from August 2000 to July 2001. Among 1055 fleas of nine species captured, four species predominated (94%). Two species of Pulicidae, Xenopsylla dipodilli Smit and X. ramesis (Rothschild), reproduced perennially, whereas adults of Nosopsyllus iranus theodori Smit (Ceratophyllidae) and Stenoponia tripectinata medialis Jordan (Hystrichopsyllidae) occurred only in cool months (October-March). During their main activity season on the most infested host species (estimated from > 300 trap-nights/month), prevalence of these four flea species reached 40-70%, 20-30%, 100% and 50-70%, respectively, with infestation intensities of 2-2.7, 7-12, 2-3.5 and 2.5-7 fleas per infested rodent, respectively. Xenopsylla dipodilli oviposition peaked during autumn with parous rate > 80% by September-October. During December-April, the majority of X. dipodilli females were immature and/or nulliparous (defined as having mature ovaries but no follicular relics). In contrast, X. ramesis had two reproductive peaks, in mid-spring and autumn, evidenced by the influx of immature females in late spring and summer (30-40%) and in winter (20-30%) after maximal parous rates: 80-100% in March-April and 95-100% in October-November. Nosopsyllus iranus theodori and Stenoponia tripectinata medialis adults occurred only during cool months. At the beginning of activity, during October-November, the sex ratio of N. i. theodori was strongly biased to females (86%) that were immature and/or nulliparous. In winter, adult females were 52-65% parous and 10-32% immature. In March, as the adult population of N. i. theodori declined, 78% of females were parous and 12% immature. Seasonal activity of S. t. medialis (November-March) was shorter than for the other three species; females were predominantly nulliparous in November (80%), after which the proportion of parous females increased gradually to 84% in February. Two females of S. t. medialis collected in March were mature but nulliparous, suggesting that this species of flea might 'oversummer' (as pupae or teneral adults) in the cocooned stage.  相似文献   

15.
The effect of both moisture and temperature on the infective potential of Beauveria bassiana to the Chagas' disease vector, Rhodnius prolixus, was studied under fluctuating regimes. At constant 25 degrees C, contaminated first-instar nymphs exposed to increasing daily periods of initial exposure to 97% RH, followed by transfer to reduced humidity (43, 53, 75, and 86% RH), showed a significant reduction in mortality when the 97% RH exposure time declined from 12 to 8 h per day. The duration of disease incubation depended on the daily 97% RH exposure time. Under fluctuating regimes of both humidity (97% RH versus 75% RH) and temperature (15/28, 20/25, 25/28, and 25/35 degrees C), first-instar mortality was affected by weather conditions, daily 97% RH exposure time (8, 12, and 16 h per day), and number of temperature and humidity fluctuations before transferring tested insects to constant unfavorable conditions. In most cases, at 12/12 h alternating cycles, high and rapid mortality required five cycles. Under these fluctuating regimes, fungus-induced mortality and mortality time were similarly affected in third- and fifth-instar nymphs by the daily 97% RH exposure time. Despite a lower susceptibility of older larval stages, mortality rates in insects exposed for at least 12 h per day at 97% RH remained very high except at 15 degrees C. Moisture and temperature regimes at 12/12 h cycling significantly affected the dose-mortality response in first-instar nymphs. The most favorable conditions consisted of 97%-20 degrees C combined with either 75%-25 degrees C or 43%-25 degrees C. Under less favorable alternating conditions (lower and higher temperatures) the amounts of inoculum required for killing 50% of first-instar nymphs were 10 or 20 times higher. From a vector control standpoint, daily high humidity appears to be the most crucial climatic constraint. B. bassiana has the potential to control R. prolixus populations with applications made during the rainy seasons when humidity is high.  相似文献   

16.
Helicoverpa armigera (Hübner) exhibits a facultative pupal diapause, which depends on temperature and photoperiod. Pupal diapause is induced at 20 degrees C by short photoperiods and inhibited by long photoperiods during the larval stage. However, in some pupae (35% of males and 57% of females) of a non-selected field population from Okayama Prefecture (34.6 degrees N), diapause is not induced by short photoperiods. In the present experiment, the importance of temperature for diapause induction was studied in the non-diapausing strain, which was selected from such individuals reared at 20 degrees C under a short photoperiod of 10L:14D. Furthermore, the sensitive stage for thermal determination of pupal diapause was determined by transferring larvae of various instars and pupae between 20 degrees C and 15 degrees C. Diapause was induced by 15 degrees C without respect to photoperiod. When larvae or pupae reared from eggs at 20 degrees C under a short or a long photoperiod were transferred to 15 degrees C in the periods of the middle fifth instar to the first three days after pupation, the diapause induction rate was significantly reduced in both males and females, especially in females. In contrast, when larvae or pupae reared at 15 degrees C were transferred to 20 degrees C in the same periods, diapause was induced in males, but not in females. However, the diapause induction rate of pupae transferred to 20 degrees C on the fourth day after pupation was significantly increased in females. The results show that temperature is the major diapause cue in the photoperiod-insensitive strain and the periods of middle fifth larval instar to early pupal stage are the thermal sensitive stages for pupal diapause induction with some different responses to temperatures between males and females in H. armigera.  相似文献   

17.
When pupae of Delia antiqua were transferred to constant darkness (DD) from light-dark (LD) cycles or constant light (LL), the sensitivity to light of the circadian clock controlling eclosion increased with age. The daily rhythm of eclosion appeared in both non-diapause and diapause pupae only when this transfer was made during late pharate adult development. When transferred from LL to DD in the early pupal stage, the adult eclosion was weakly rhythmic in non-diapause pupae but arrhythmic in diapause pupae. However, the sensitivity of the circadian clock to temperature cycles or steps was higher in diapause pupae than in non-diapause pupae; in the transfer to a constant 20 degrees C from a thermoperiod of 25 degrees C (12 h)/20 degrees C (12 h) on day 10 after pupation or from chilling (7.5 degrees C) in DD, the adult eclosion from diapause pupae was rhythmic but that from non-diapause pupae arrhythmic. In a transfer to 20 degrees C from the thermoperiod after the initiation of eclosion, rhythmicity was observed in both types of pupae. The larval stage was insensitive to the effect of LD cycle initiating the eclosion rhythm. In D. antiqua pupae in the soil under natural conditions, therefore, the thermoperiod in the late pupal stage would be the most important 'Zeitgeber' for the determination of eclosion timing.  相似文献   

18.
During 1992, the population dynamics of rabbit fleas were compared at two sites in north-eastern Spain. The sites differed mainly in terms of annual rainfall and soil type. All flea species showed seasonal cycles of abundance, although peaks in numbers occurred at different times, reflecting their specific adaptations for coping with climatic variables. Adult Spilopsyllus cuniculi (Dale) (Siphonaptera: Pulicidae) were found largely parasitizing rabbits in spring and adult Caenopsylla laptevi (Beaucournu etal.) (Siphonaptera: Ceratophyllidae) in the autumn. In contrast, monthly flea indices of Xenopsylla cunicularis (Smit) (Siphonaptera: Pulicidae) and Echidnophaga iberica (Ribeiro et al.) (Siphonaptera: Pulicidae) peaked in summer. Spilopsyllus cuniculi was present at both sites, but was less common on the drier site, where monthly mean temperature and annual rainfall approached the flea's physiological limits. By contrast, E. iberica, X. cunicularis and C. laptevi, known to be better adapted for dryness, showed the opposite patterns of abundance. Nevertheless, even these arid-adapted species took advantage of the milder and wetter spring (X. cunicularis and E. iberica) or autumn (C. laptevi) for breeding and larval development. Although environmental temperature, rainfall and soil texture will influence the microclimate of the burrows where the flea larvae develop, burrow humidity seems to be more dependent on soil characteristics and past rainfall rather than the humidity of the external air.  相似文献   

19.
In tropical areas, where vector insects populations are particularly numerous, temperature usually range between 25 degrees C and 35 degrees C. Considering the importance of such temperature variation in determining mosquitoes population dynamics, in this work the developmental, eclosion and survival rates of the immature stages of Aedes albopictus (Skuse) were compared under constant 25, 30 and 35 degrees C (using acclimatized chambers) and environmental (25 degrees C to 29 degrees C) temperatures. The hatching rate was considered as total number of larvae recovered after 24h. The development period as well as larval and pupal survival rate were evaluated daily. Eclosion rate was significantly higher under environmental temperature than under the studied constant temperatures, suggesting that temperature variation may be an eclosion-stimulating factor. The mean eclosion time increased with the temperature, ranging from 2.8h (25 degrees C) to 5.2h (35 degrees C). The larval period was greatly variable inside each group, although it did not differ significantly amongst groups (11.0 +/- 4.19 days), with individuals showing longer larval stages in water at 35 degrees C (12.0 +/- 4.95 days) and environmental temperature (13.6 +/- 5.98 days). Oppositely, survival was strongly affected by the higher temperature, where only one individual lived through to adult phase. The results suggest that population of Ae. albopictus from Recife may be adapting to increasing of environmental temperatures and that the limiting temperature to larval development is around 35 degrees C.  相似文献   

20.
The rearing of Galleria mellonella L. in laboratory is important for multiplication of entomopathogenic nematodes, mandatory for biological control studies. The objective of this study was to evaluate the effect of three thermal profiles on development stages of this insect, allowing synchronization of cycle production. Two distinct rearing phases were done: firstly, using nucleous of incubation for development of eggs and, secondly, using circular-aluminum manifolds for development of larvae and pupae. The time necessary for development of the immature stages decreased with higher temperatures. Incubation periods lasted 13.4 days at 22 degrees C, 8.3 at 27 degrees C and 6.8 days at 32 degrees C, while periods for larvae development lasted 40.4, 27.2, and 23.4 days, respectively, for the same temperatures. Development to pupal stage was observed 18.2, 15.0, and 12.2 days, respectively, for the same temperatures. Larval survival was higher at 32 degrees C, however embryonic stages and pupae survival were higher at 27 degrees C. and 22 degrees C, respectively. The threshold temperature was 11.209167 degrees C for the embryonic development stage, 7.695869 degrees C for larval stage, and 1.943050 degrees C for pupal stage of G. mellonella. Thermal constants were 138.380533 DG (degree day) for egg, 554.968830 DG for larvae, and 369.054080 DG for pupae.  相似文献   

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