共查询到20条相似文献,搜索用时 15 毫秒
1.
Linh Nguyen Thi Nhat Cuong Le Kim Tam Ho Thanh Tung Hoang Thanh Luan Vu Quoc Hien Vu Thi Loc Nguyen Hoang Nhut Duong Tan 《Plant Cell, Tissue and Organ Culture》2019,136(1):101-108
Plant Cell, Tissue and Organ Culture (PCTOC) - The effects of l-alanine and its derivatives on cephalotaxine production of Cephalotaxus mannii suspension cultures were investigated in this paper.... 相似文献
2.
We investigated the effects on ginseng adventitious root growth and ginsenoside production when macro-element concentrations
and nitrogen source were manipulated in the culture media. Biomass growth was greatest in the medium supplemented with 0.5-strength
NH4PO3, whereas ginsenoside accumulation was highest (9.90 mg g-1 DW) in the absence of NH4PO3. At levels of 1.0-strength KNO3, root growth was maximum, but a 2.0 strength of KNO3 led to the greatest ginsenoside content (9.85 mg g-l). High concentrations of MgSO4 were most favorable for both root growth and ginsenoside accumulation (up to 8.89 mg g-1 DW). Root growth and ginsenoside content also increased in proportion to the concentration of CaCI2 in the medium, with the greatest accumulation of ginsenoside (8.91 mg g-1 DW) occurring at a 2.0 strength. The NH4/NO3
-- ratio also influenced adventitious root growth and ginsenoside production; both parameters were greater when the NO3
- concentration was higher than that of NH4
+. Maximum root growth was achieved at an NH4
+/NO3
- ratio of 7.19/18.50, while ginsenoside production was greatest (83.37 mg L-1) when NO3
- was used as the sole N source. 相似文献
3.
Effects of elicitation on the production of saponin in cell culture of Panax ginseng 总被引:1,自引:0,他引:1
This study was initiated to investigate the impacts of elicitor concentration and elicitor-adding time on the saponin synthesis and the cell growth of Panax ginseng cell suspensions. Both of the elicitors tested, yeast extract and methyl jasmonate, significantly improved saponin production. The highest additive level of the seven ginsenosides tested was 2.07% (dry weight basis), which was 28-fold higher than that in the control. The optimum time to add either elicitor was found to be on the day of inoculation. The addition of either elicitor did not show as significant an influence on cell growth as on saponin production. It was advisable to remove 2,4-dichlorophenoxyacetic acid (2,4-D) from the medium when methyl jasmonate was used as the elicitor as methyl jasmonate interacts antagonistically with 2,4-D. These results suggest that the addition of an elicitor to ginseng cell suspension cultures could stimulate saponin production. 相似文献
4.
Involvement of nitric oxide-induced NADPH oxidase in adventitious root growth and antioxidant defense in Panax ginseng 总被引:1,自引:0,他引:1
Rajesh Kumar Tewari Soohyun Kim Eun-Joo Hahn Kee-Yoeup Paek 《Plant biotechnology reports》2008,2(2):113-122
Nitric oxide (NO) affects the growth and development of plants and also affects plant responses to various stresses. Because
NO induces root differentiation, we examined whether or not it is involved in increased ROS generation. Treatments with sodium
nitroprusside (SNP), an NO donor, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), a specific NO scavenger,
and Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME), an NO synthase (NOS) inhibitor, revealed that NO is involved in the adventitious root growth of mountain ginseng.
Supply of an NO donor, SNP, activates NADPH oxidase activity, resulting in increased generation of O2
·−, which subsequently induces growth of adventitious roots. Moreover, treatment with diphenyliodonium chloride (DPI), an NADPH
oxidase inhibitor, individually or with SNP, inhibited root growth, NADPH oxidase activity, and O2
·− anion generation. Supply of the NO donor, SNP, did not induce any notable isoforms of enzymes; it did, however, increase
the activity of pre-existing bands of NADPH oxidase, superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, and
glutathione reductase. Enhanced activity of antioxidant enzymes induced by SNP supply seems to be responsible for a low level
of H2O2 in the adventitious roots of mountain ginseng. It was therefore concluded that NO-induced generation of O2
·− by NADPH oxidase seems to have a role in adventitious root growth of mountain ginseng. The possible mechanism of NO involvement
in O2
·− generation through NADPH oxidase and subsequent root growth is discussed. 相似文献
5.
Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate 总被引:2,自引:0,他引:2
Adventitious roots of ginseng were treated with methyl jasmonate (MJ) up to 150 microM and cultured for 40 days. Up to 100 microM MJ inhibited the root growth but increase ginsenoside accumulation. In a two-stage bioreactor culture, total ginsenosides, after elicitation with 100 microM MJ peaked after 10 days at 48 mg g(-1) dry wt and then dropped sharply. Of the two groups of ginsenosides (Rb and Rg), higher amounts of Rb accumulated in the adventitious roots. 相似文献
6.
Kee-Won Yu Wen-Yuan Gao Sung-Ho Son Kee-Yoeup Paek 《In vitro cellular & developmental biology. Plant》2000,36(5):424-428
Summary Hairy root cultures of Panax ginseng, established after the infection of root sections with Agrobacterium rhizogenes KCTC 2703, were cultured in phytohormone-free Murashige and Skoog (MS) liquid medium containing different concentrations
of jasmonic acid and some other elicitors, in order to promote ginsenoside accumulation. Jasmonic acid in the range 1.0−5.0
mg l−1 (4.8–23.8 μM) strongly improved total ginsenoside production in ginseng hairy roots. Peptone (300 mg l−1) also showed some effect on ginsenoside improvement; however its effect was much weaker than that of jasmonic acid. Ginsenoside
content and productivity were 58.65 and 504.39 mg g−1, respectively. The Rb group of ginsenoside content was increased remarkably by jasmonic acid, while Rg group ginsenoside
content changed only slightly compared to controls. However, jasmonic acid also strongly inhibited ginseng hairy root growth. 相似文献
7.
植物激素对人参毛状根生长和皂甙含量的影响 总被引:14,自引:0,他引:14
就植物激素IAA、IBA、NAA、2,4-D对人参(Panax ginseng C.A.Meyer)毛状根生长及皂甙含量的影响进行了研究。结果表明,4种生长素在适宜的浓度下均可不同程度地促进人参毛状根的生长以及皂甙的积累,同时能影响单体皂甙的分布。NAA和IBA能显著促进毛状根的生长,其中0.500mg/L IBA能显著促进毛状根生长和总皂甙的积累。细胞分裂素6-BA在较低浓度时虽然对生长无明显的促进作用,但对皂甙积累有利,同时显著促进单体皂甙Rb1的积累,增大Rb1在总甙中所占的比例。 相似文献
8.
Jong-Du Lee Kim-Cuong Le Young-Ki Park Hosakatte Niranjana Murthy Kee-Yoeup Paek So-Young Park 《Plant Cell, Tissue and Organ Culture》2018,132(2):295-302
Panax ginseng and Panax quinquefolius of Panax genus are valuable as health foods as well as pharmaceuticals for the treatment of cancer, diabetes and ageing as these plants possess saponins. In the current study, Cell and adventitious root cultures of P. ginseng and P. quinquefolius were investigated for the biomass, cell division, saponin content and ginsenosides profile from four lines namely P. quinquefolius (AM), P. ginseng mountain (Mt.) Baekdu line, P. ginseng Cheong-sol line (CS) and P. ginseng CBN line (CBN) with the objective of comparing cell and adventitious root systems to check their efficacy for the production of ginseng saponins. Additionally, genes related to ginsenoside biosynthesis were also analyzed concerning to cell and adventitious root lines. The results indicated that various cell lines were better in multiplication and growth compared to adventitious root lines. However, adventitious root lines showed higher accumulation of dry biomass (1.5–2 fold) than that of cell lines. CS adventitious root line showed higher saponin content and ginsenoside productivity (10.48 mg·g?1 DW, 12.88 mg·L?1, respectively) than that of CS cell line (9.50 mg·g?1 DW, 2.39 mg·L?1, respectively). Especially, Rd ginsenoside productivity of CS adventitious root line recorded fourfold higher than CS cell line. Genes which are related to ginsenoside biosynthesis such as P. ginseng squalene synthase (PgSS2), P. ginseng squalene epoxidase (PgSE2), P. ginseng protopanaxadial synthase (PgPPDS) and P. ginseng protopanaxatriol synthase (PgPPTS) were analyzed by real time quantitative polymerase chain reaction to support ginsenoside production. The adventitious root culture system described in this study is useful system for biomass and ginsenoside production. 相似文献
9.
Indole-3-butyric acid at 25 μM with methyl jasmonate (MJ) at 100 μM in Panax ginseng synergistically stimulated both root growth and ginsenoside accumulation compared with 100 μM MJ alone. Productivity of ginsenoside
was 10 mg l−1 d−1 compared to 7.3 mg l−1 d−1 with MJ elicitation alone. 相似文献
10.
The effects of methyl jasmonate (MJ) elicitation on the cell growth and accumulation of ginsenoside in 5-l bioreactor suspension cultures of Panax ginseng were investigated. Ginsenoside accumulation was enhanced by elicitation by MJ (in the range 50–400 M); however, fresh weight, dry weight and growth ratio of the cells was strongly inhibited by increasing MJ concentration. The highest ginsenoside yield was obtained at 200 M MJ. In the second experiment, 200 M MJ was added on day 15 during the cultivation. The ginsenoside, Rb group, and Rg group ginsenoside content increased 2.9, 3.7, and 1.6 times, respectively, after 8 days of MJ treatment. Rb group gisnsenosides accumulated more than Rg group ginsenosides. Among Rb group ginsenosides, Rb1 content increased significantly by four times but the contents of Rb2, Rc and Rd increased only slightly. Among Rg group ginsenosides, Rg1 and Re showed 2.3-fold and 3.0-fold increments, respectively, whereas there was only a slight increment in Rf group ginsenosides. These results suggest that MJ elicitation is beneficial for ginsenoside production using 5-l bioreactor cell suspension cultures. 相似文献
11.
Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots 总被引:1,自引:0,他引:1
Shinji Inomata Mineyuki Yokoyama Yoko Gozu Toshiaki Shimizu Mitsuo Yanagi 《Plant cell reports》1993,12(12):681-686
Panax ginseng roots transformed by Agrobacterium rhizogenes grew rapidly in a hormone-free medium. The transformed roots showed biphasic growth: rapid during the first two weeks and slower thereafter. Sucrose in the medium was almost all converted to glucose and fructose during the first two weeks, and the root growth slowed down after the depletion of sucrose in the medium. Periodic changes of the medium maintained the high growth rate, and the dry weight increased by 31 times in 32 days, which is the highest growth rate so far reported for cultured tissues of ginseng. The medium exchange also increased the ginsenoside content in the roots. Effective scale-up of the root culture was achieved in a turbine-blade type bioreactor. 相似文献
12.
Isao Asaka Ichio Ii Masao Hirotani Yoshihisa Asada Tsutomu Furuya 《Biotechnology letters》1993,15(12):1259-1264
Summary Ginseng (Panax ginseng) embryogenic tissues were cultured in three types of reactors and the ginsenoside productivities in these tissues were compared. As a result, the saponin productivity was the best when an airlift reactor was used, and more than twice of that when a paddle or internal turbine reactor was used. The tissues grew 9 fold during 42 days, and the ginsenoside pattern resembled that of ginseng leaves.Part 98 in the series Studies on Plant Tissue Cultures For Part 97 see Orihara, Y., and Furuya, T., (1993) submitted for publication. 相似文献
13.
Rajesh Kumar Tewari Soo Yeon Lee Eun Joo Hahn Kee Yoeup Paek 《Plant biotechnology reports》2007,1(4):227-235
Nitric oxide (NO) is a diffusible, gaseous signaling molecule. In plants, NO influences growth and development, and it can
also affect plant responses to various stresses. Because NO induces root differentiation and interacts with reactive oxygen
species, we examined the temporal effect of NO elicitation on root growth, saponin accumulation and antioxidant defense responses
in the adventitious roots of mountain ginseng (Panax ginseng). The observations revealed that NO is involved in root growth and saponin production. Elicitation with sodium nitroprusside
(SNP) activated O2
−-generating NADPH oxidase (NOX) activity, which most probably subsequently enhanced growth of adventitious roots of mountain
ginseng. A severe inhibition of NOX activity and decline in dry weight of SNP elicited adventitious roots in the presence
of NOX inhibitor (diphenyl iodonium, DPI), which further supports involvement of NOX in root growth. Enhanced activities of
antioxidant enzymes by SNP appear to be responsible for low H2O2, less lipid peroxidation, and modulation of ascorbate and non-protein thiol statuses in the adventitious roots of mountain
ginseng. Dry mass, saponin content and NOX activity was related with NO content present in adventitious roots of mountain
ginseng. 相似文献
14.
15.
Influence of auxins on organogenesis and ginsenoside production in Panax ginseng calluses 总被引:2,自引:0,他引:2
Bonfill Mercedes Cusidó Rosa M. Palazón Javier Piñol M. Teresa Morales Carmen 《Plant Cell, Tissue and Organ Culture》2002,68(1):73-78
Panax ginseng calluses were cultured for 5 weeks on solid MS medium supplemented with kinetin 0.46 mM (0.1 mg l–1) and 2 mg l–1 of 2,4-D (9.05 mM), IBA (9.98 mM) or NAA (10.74 mM). In the conditions studied, 2,4-D inhibited the organogenic capacity of the calluses, whereas IBA or NAA increased this capacity. IBA induced the formation of a high number of buds and roots, but the roots were thin and necrotized. Calluses grown with NAA produced fewer buds and roots than those grown in IBA medium, but the roots were thick and showed good growth. The highest ginsenoside content was found in root forming calluses grown in the presence of NAA.In calluses forming roots or buds, 2,4-D, NAA and especially IBA increased the Rb group of ginsenosides rather than that of the Rg group. 相似文献
16.
17.
Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng 总被引:1,自引:0,他引:1
Adventitious roots ofPanax ginseng C.A. Meyer (a natural tetraploid) were treated with 50 or 100 mg L-1 colchicine for 12, 24,36, 48, or 60 h to induce polyploid (octoploid) roots. The largest number of octoploid roots was obtained
with a 100 mg L-1 colchicine treatment over 60 h. To verify that ginsenoside was being accumulated in the developing tissues, the tetraploid
(control) and octoploid roots were cultured for 40 d in Murashige and Skoog media that lacked NH4NO3 but was supplemented with 2 mg L-1 naphthaleneacetic acid and 50 g L-1 sucrose. Levels of fresh and dry biomass were greater in the octoploid roots. Although total ginsenoside and Rb-group ginsenoside
contents were less in the octoploid roots than in the tetraploids, the former had a higher amount of Rg-group ginsenosides
(especially Rg1). These results demonstrate the benefit that polyploid adventitious roots provide in enhancing the production
of secondary metabolites in ginseng. 相似文献
18.
19.
Choi Sung Mee Ho Son Sung Rho Yun Seung Woung Kwon Oh Hoon Seon Jeong Yoeup Paek Kee 《Plant Cell, Tissue and Organ Culture》2000,62(3):187-193
A pilot-scale culture of multiple adventitious roots of ginseng was established using a balloon-type bubble bioreactor. Adventitious
roots (2 cm) induced from callus were cultured in plastic Petri dishes having 20 ml of solid Schenk and Hildebrandt (1972)
medium containing 3% sucrose, 0.15% gelrite, and 24.6 μM indole-3-butric acid. An average of 29 secondary multiple adventitious
roots were produced after 4 weeks of culture. These secondary roots were elongated on the same medium, reaching a length of
5 cm after 6 weeks of culture. A time course study revealed that maximum yields in 5-l and 20-l bioreactors were approximately
500 g and 2.2 kg at day 42 with 60 g and 240 g inoculations, respectively. Cutting twice during the culture increased the
total amount of biomass produced. The root biomass in a 20-l balloon-type bubble bioreactor was 2.8 kg at harvest with 240
g of inoculum after 8 weeks of culture. The total saponin content obtained from small-scale and pilot-scale balloon type bubble
bioreactors was around 1% based on dry weight. Inoculation of 500 g fresh weight of multiple adventitious roots into a 500
l balloon-type bubble bioreactor with cutting at 4 and 6 weeks after inoculation produced approximately 74.8 kg of multiple
roots. The ginsengnoside profiles of these multiple adventitious roots were similar to profiles of field-grown ginseng roots
when analyzed by HPLC.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
20.
Six new protopanaxadiol-type ginsenosides, named ginsenosides Ra(4) -Ra(9) (1-6, resp.), along with 14 known dammarane-type triterpene saponins, were isolated from the root of Panax ginseng, one of the most important Chinese medicinal herbs. The structures of the new compounds were determined by spectroscopic methods, including 1D- and 2D-NMR, HR-MS, and chemical transformation as (20S)- 3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[β-D-xylopyranosyl-(1→4)-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (1), (20S)-3-O-[β-D-6-O-acetylglucopyranosyl-(1→2)-β-D-glucopyranosyl]-20-O-[β-D-xylopyranosyl-(1→4)-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (2), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (3), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (4), (20S)-3-O-{β-D-4-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (5), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (6). The sugar moiety at C(3) of the aglycone of each new ginsenoside is butenoylated or acetylated. 相似文献