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宝兴泛树蛙和昭觉泛树蛙的染色体学研究,兼论两种的分类现状 总被引:2,自引:0,他引:2
本文选择宝兴泛树蛙模式标本产地四川宝兴居群(背部有斑点和无斑点两种类型)、大瓦山居群、瓦屋山居群,也选择了被命名为昭觉泛树蛙的模式标本产地四川昭觉的居群,共两种泛树蛙4个居群的5类52号标本,进行染色体组型、次缢痕及Ag-NORs研究。本文结果主要有三:1、首次报道宝兴泛树蛙地模标本的染色体组型,且证明背部有斑点与无斑点个体在染色体学方面没有显著区别;因此将过去文献上对宝兴泛树蛙色斑的描述作了补充。2、对昭觉泛树蛙昭觉居群的研究,证实吴贯夫与曾晓茂(1994)关于昭觉居群是与宝兴泛树蛙形态虽然相似,但染色体特征之间有极显著差别的另一个种,但按照“优先权律”,本文正式恢复刘承钊根据昭觉燕窝塘标本命名的胡氏泛村蛙学名的有效性,同时将吴与曾命名的昭觉泛树蛙学名作为前者的次定同物异名。3、本文所研究的宝兴泛树蛙属于广泛分布的种,建议有必要补充其它居群(如四川巴塘居群)的染色体学资料,再作进一步的分析是否尚有隐存种(或亲缘种)的存在。 相似文献
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宝兴泛树蛙不同地理居群的染色体多样性 总被引:4,自引:0,他引:4
本文分析了比较了宝兴泛树蛙四个地理居群的核型,C-带和Ag-NORs,结果表明它们有相同的2n=26,NF=52,和相同的核型模式:5+8。但是,它们彼此之间在SM数目和顺序,次缢痕或随体的位置以及银染的位置,C-带类型都有所不同。 相似文献
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1996 年5 月在云南普洱采到11 号树蛙科标本, 经研究鉴定订为新种普洱泛树蛙, 在形态上它与杜氏泛树蛙相近, 但有较大差异。 相似文献
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寄生于杜氏泛树蛙多盘虫属单殖吸虫一新种记述 总被引:5,自引:5,他引:0
于云南屏边县杜氏泛树蛙Polypedates dugritei David膀胱内检获单殖吸虫多盘科多盘虫属1新种:屏边多盘吸虫Polystoma pingbianensis sp.nov.,对其形态结构及宿主特异性问题进行了初步研究.据宿主膀胱内寄生的产卵成虫测量形态分类数据和描述鉴定特征.虫体全长平均9.428 mm,后吸器大小平均0.869 mm×0.784 mm,后吸器吸盘的直径平均434.4μm,后吸器上两个中央大钩全长平均403.2μm.朝向外侧肠管无盲突,朝向内侧肠管每侧形成13~14个盲突,有1个或无过腹中线的肠联合,肠管于后端合并向后延伸至后吸器内,不形成盲突.卵巢较小,位于单个睾丸之前,卵巢长平均711.52 μm.卵相对较大,卵长平均306.6μm,卵宽平均164.6μm. 相似文献
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研究两栖类物种雌雄间的形态差异,不仅有助于理解物种两性异形的模式与机制,而且对其繁殖策略、繁殖投入和进化选择压力的研究都具有重要意义。2020年4月至2021年8月以浙江省丽水市丽水学院校园永久池塘内的斑腿泛树蛙(Polypedates megacephalus)为实验对象,共61只(♀12,♂49),测量其头体长、头长、吻宽、前肢长、后肢长等12项形态特征及体重来研究该物种的两性异形,并收集窝卵数和孵化数据分析雌性繁殖能力。结果表明:斑腿泛树蛙具有显著的两性异形,且雌性个体大于雄性个体;眼径、胫长和鼻间距在两性间无显著差异,头体长、头长、吻宽、吻长、眼间距、前肢长、后肢长、前臂及手长、足长和体重均具有显著差异;斑腿泛树蛙雌性个体产卵和孵化率受季节影响显著,其中4和5月窝卵数最多,6月卵孵化率最高;雌性怀卵数量与体长呈正相关关系。依据上述结果,本研究推测生育力选择是造成斑腿泛树蛙两性异形的主要因素,雌性个体通过增大个体体型来增加怀卵量,从而提高繁殖输出;斑腿泛树蛙繁殖期受季节影响,为更好地适应雨季的提前或推迟,在进化过程中形成了延长式繁殖的模式。 相似文献
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A novel bioactive peptide (polypedarelaxin 1) was identified from the skin secretions of the tree frog, Polypedates pingbianensis. Polypedarelaxin 1 is composed of 21 amino acid residues with a sequence of QGGLLGKVSNLANDALGILPI. Its primary structure was further confirmed by cDNA cloning and mass spectrometry analysis. Polypedarelaxin 1 was found to elicit concentration-dependent relaxation effects on isolated rat ileum. It has no antimicrobial and serine protease inhibitory activities. BLAST search revealed that polypedarelaxin 1 did not show similarity to known proteins or peptides. Especially, polypedarelaxin 1 do not contain conserved structural motifs of other amphibian myotropic peptides, such as bradykinins, bombesins, cholecystokinin (CCK), and tachykinins, indicating that polypedarelaxin 1 belongs to a novel family of amphibian myotropic peptide. 相似文献
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本底资源是保护区建设与发展的基础,也是生物多样性深入研究与保护重要的参考资料。湖南小溪国家级自然保护区是我国中南地区具有代表性的原始次生林区,生物资源相对丰富。自2014年以来,对区内的两栖爬行动物资源、多样性及区系进行了较为系统的调查与研究。结果表明:区内现有两栖爬行动物2目16科40属63种,其中两栖动物1目7科15属33种,爬行动物1目9科25属30种;无声囊泛树蛙Polypedates mutus和老山树蛙Rhacophorus laoshan是湖南省两栖动物新记录;核心区的物种多样性指数和均匀度指数均高于缓冲区和实验区;动物区系成分缺乏古北界种类,以东洋界种类为主(48种,占76.2%),广布种有15种(23.8%);平均动物区系相似性比较发现,其与湖南省内其他6个保护区(森林公园)属于周缘关系。鉴于保护区两栖爬行动物资源的丰富性、典型性与代表性,应加强研究和保护。 相似文献
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A novel myotropic peptide, polypedatein, was purified and characterized from the skin secretions of the tree frog, Polypedates pingbianensis. Its primary structure, TLLCKYFAIC, was determined by Edman degradation and mass spectrometry. Polypedatein was subjected to bioassays including myotropic, antimicrobial, and serine protease inhibitory activities, which are related with many amphibian skin bioactive peptides. It was found to elicit concentration-dependent contractile effects on isolated rat ileum. cDNA clones encoding the precursor of polypedatein were isolated by screening a skin cDNA library of P. pingbianensis and then sequenced. The amino acid sequence deduced from the cDNA sequences matches well with the result from Edman degradation. BLAST search revealed that the sequence of polypedatein did not show similarity to known protein or peptide sequences. Especially, polypedatein does not contain conserved structural motifs of other amphibian myotropic peptides, such as bradykinins, bombesins, cholecystokinin (CCK), and tachykinins, indicating that polypedatein belongs to a novel amphibian myotropic peptide family. The signal peptide of the precursor encoding polypedatein shows significant sequence identity to that of other amphibian skin defensive peptides, such as antimicrobial peptides, bradykinins, lectins, and serine protease inhibitors, suggesting that polypedatein belongs to a novel amphibian myotropic peptide family. Polypedatein is also the first bioactive peptide from the genus of the frog, Polypedates. 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献