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1.
Ahlert Schmidt 《Archives of microbiology》1977,112(3):263-270
Crude extracts of Rhodospirillum rubrum catalyzed the formation of acid-volatile radioactivity from (35S) sulfate, (35S) adenosine-5-phosphosulfate, and (35S) 3-phosphoadenosine-5-phosphosulfate. An enzyme fraction similar to APS-sulfotransferases from plant sources was purified 228-fold from Rhodospirillum rubrum. It is suggested here that this enzyme is specific for adenosine-5-phosphosulfate, because the purified enzyme fraction metabolized adenosine-5-phosphosulfate, however, only at a rate of 1/10 of that with adenosine-5-phosphosulfate. Further, the reaction with 3-phosphoadenosine-5-phosphosulfate was inhibited with 3-phosphoadenosine-5-phosphate whereas this nucleotide had no effect on the reaction with adenosine-5-phosphosulfate. For this activity with adenosine-5-phosphosulfate the name APS-sulfotransferase is suggested. This APS-sulfotransferase needs thiols for activity; good rates were obtained with either dithioerythritol or reduced glutathione; other thiols like cysteine, 2-3-dimercaptopropanol or mercaptoethanol are less effective. The electron donor methylviologen did not catalyze this reaction. The pH-optimum was about 9.0; the apparent K
m for adenosine-5-phosphosulfate was determined to be 0.05 mM with this so far purified enzyme fraction. Enzyme activity was increased with K2SO4 and Na2SO4 and was inhibited by 5-AMP. These properties are similar to assimilatory APS-sulfotransferases from spinach and Chlorella.Abbreviations APS
adenosine-5-phosphosulfate
- PAPS
3-phosphoadenosine-5-phosphosulfate
- 5-AMP
adenosine-5-monophosphate
- 3-AMP
adenosine-3-monophosphate
- 3-5-ADP
3-phosphoadenosine-5-phosphate (PAP)
- DTE
dithiorythritol
- GSH
reduced glutathione
- BAL
2-3-dimercaptopropanol 相似文献
2.
Domenico Acquotti Giovanni Fronza Laura Riboni Sandro Sonnino Guido Tettamanti 《Glycoconjugate journal》1987,4(2):119-127
The complete definition of the chemical structure of GD1b-ganglioside (GD1b) lactone isolated from human brain has been given by means of spectrometric and spectroscopic analyses. GD1h lactone contains a single ester linkage involving the external sialic acid carboxyl group and the C-9 hydroxyl group of the internal sialic acid unit. A synthetic lactone of GD1b prepared treating GD1b with glacial acetic acid characterized in the same way showed an identical chemical structure.Abbreviations: Ganglioside nomenclature is according to Svennerholm [16] and the IUPAC-IUB Recommendations [17] GM1
GM1-ganglioside, II3NeuAc-GgOse4Cer, Gal1-3GalNac1-4[NeuAc2-3]Gal1-4Glc1-1Cer
- GD1b
GD1b-ganglioside, II3(NeuAc)2GgOse4Cer, Gal1-3GalNAc1-4[NeuAc2-8NeuAc2-3]Gal1-4Glc1-1Cer
- GD1b lactone
GD1b-L, Gal1-3GalNAc1-4[NeuAc(1-9)2-8NeuAc2-3]Gal1-4Glc1-1Cer
- Cer
ceramide
- FAB-MS
fast atom bombardment-mass spectrometry
-
1H-NMR
proteon nuclear magnetic resonance
- 1D-NMR
one dimensional NMR
- 2D-COSY
two dimensional correlated spectroscopy
- DMSO-d6
deuterated dimethylsulfoxide 相似文献
3.
Summary Imidazolides of dinucleotides such as ImpApA can be formed from the corresponding dinucleotides in a two-stage process, which gives up to 15% yields under potentially prebiotic conditions. First a solution of the dinucleotide and sodium trimetaphosphate is dried out at constant temperature and humidity. This produces polyphosphates such as pnApA in excellent yield (80%). The products are dissolved in water, imidazole is added, and the solution is dried out again. This yields the 5-phosphorimidazolides.Abbreviations P3!
trimetaphosphate
- A
adenosine
- U
uridine
- EDTA
ethylenediaminetetraacetic acid
- Ap
adenosine 2(3)-phosphate
- Ap!
adenosine cyclic 2:3-phosphate
- pA
adenosine 5-phosphate
- pA2p
adenosine 2, 5-diphosphate
- pA3p
adenosine 3, 5-diphosphate
- pAp!
5-phospho-adenosine cyclic 2:3-phosphate
- ATP
adenosine 5-triphosphate
- ImpA
adenosine 5-phosphorimidazolide
- A2pA
adenylyl-[25]-adenosine
- A3pA
adenylyl-[35]-adenosine
- A2pU
adenylyl-[25]-uridine
- A3pU
adenylyl-[35]-uridine
- pA2pA
5-phosphoadenylyl-[25]-adenosine
- pA3pA
5-phospho-adenylyl-[35]-adenosine
- pA2pU
5-phospho-adenylyl-[25]-uridine
- pA3pU
5-phospho-adenylyl-[35]-uridine
- pApN (N= A, U)
5-phosphate of a dinucleoside phosphate
- pnApN (N = A, U; n = 2, 3, 4.)
5-polyphosphate of a dinucleoside phosphate
- ImpA2pA
imidazolide of pA2pA
- ImpA3pA
imidazolide of pA3pA
- ImpA2pU
imidazolide of pA2pU
- ImpA3pU
imidazolide of pA3pU
- ImpApN
imidazolide of pApN 相似文献
4.
Wayne Paul Maddison 《Chromosoma》1982,85(1):23-37
Observations of male meiosis and female chromosome number indicate that eight species of Pellenes have the X1X2O male, X1X1X2X2 female sex chromosome system typical of salticids, four species have an X1X2X3Y male, X1X1X2X3X3X3 female system, and one species has both X1X2O and X1X2X3Y males. This is the first report of a Y chromosome in spiders. It is hypothesized that the X1X2X2Y system was derived from an X1X2O system by a tandem X-autosome fusion which yielded the X2 and a centric autosome-autosome fusion which yielded the Y. Data on heteropycnosis, chiasmata, segregation, chromosome number and arm length support this hypothesis. The distribution of the X1X2X3Y system within the genus is phylogenetically confusing and suggests that the two sex chromosome systems have been maintained together as a polymorphism in some lineages for long periods of time or that there have been repeated derivations of the X1X2X3Y or X1X2O systems. 相似文献
5.
D Richter 《Molecular & general genetics : MGG》1980,178(2):325-327
Summary The spoT gene product from Escherichia coli, the guanosine 3,5-bis(diphosphate) 3-pyrophosphohydrolase [ppGppase] catalyzes the specific release of pyrophosphate from the 3-position of guanosine 3,5-bis(diphosphate) [ppGpp]; this reaction is significantly inhibited in the presence of uncharged tRNA
yeast
Phe
. Little or no inhibition is observed with Phe-tRNAPhe, tRNAPhe-CpCpAoxi-red or ribosomal RNA (16S and 23S). 相似文献
6.
Summary We have studied the reactions between adenosine 5-phosphorimidazolide and 9-(2-amino-2-deoxyxylofuranosyl) adenine (I) or 3-methylamino-3-deoxyadenosine (II), both with and without a poly (U) template. We find that both amino compounds react much more rapidly than does adenosine, in the absence of a template. The rate of reaction is greatly enhanced by a poly (U) template in the case of I, but the enhancement is slight in the case of II.Abbreviations A
adenosine
- xylo ANH2
9-(2-amino-2-deoxy--D-xylofuranosyl) adenine
- ANHMe
3-methylamino-3-deoxyadenosine
- ImpA
adenosine 5-phosphorimidazolide
- A3 pA
adenylyl-[35]-adenosine
- A2 pA
adenylyl-[25]-adenosine
- UNPA
adenylyl-[52]-2-amino-2-deoxyuridine
- xylo ANPA
9-[adenylyl-(52)-2-amino-2-deoxy--D-xylofuranosyl]adenine
- A(NMe)pA
adenylyl-[53]-3-methylamino-3-deoxyadenosine
- pA
adenosine 5phosphate
- AppA
P1, P2-diadenosine 5pyrophosphate
- (pA)n
n = 2, 3 [2-5]-linked oligomers of pA
- A2 pA2 pA
[2-5]-linked trinucleoside diphosphate of A
- poly (U)
polyuridylic acid 相似文献
7.
Summary The two main carotenoids of Stigmatella aurantiaca were identified as 1,2-dihydro-1-hydroxy-3,4-dehydro-torulene glucoside (myxobactin) and 1,2-dihydro-1-hydroxy-4-keto-torulene glucoside (myxobacton). Both pigments occur as monoesters of various fatty acids. The structural formulas were established by chemical and chromatographical analysis and by visible, infrared, and mass spectroscopy. 相似文献
8.
Bennett T. Farmer II Luciano Müller Edward P. Nikonowicz Arthur Pardi 《Journal of biomolecular NMR》1994,4(1):129-133
Summary A set of three 3D (1H, 13C, 15N) triple-resonance correlation experiments has been designed to provide H1-H8 intraresidue sugar-to-base correlations in purines in an unambiguous and efficient manner. Together, the HsCsNb, HsCs(N)bCb, and HbNbCb experiments correlate the H1 sugar proton to the H8 proton of the attached base by means of the {H1, C1, N9, C8, H8} heteronuclear scalar coupling network. The assignment strategy presented here allows for unambiguous H1-H8 intraresidue correlations, provided that no two purines have both the same H1 and C1 chemical shifts and the same C8 and N9 chemical shifts. These experiments have yielded H1-H8 intraresidue sugar-to-base correlations for all five guanosines in the [13C, 15N] isotopically labeled RNA duplex r(GGCGCUUGCGUC)2. 相似文献
9.
Summary Short oligocytidylates can act as templates for the self-condensation of guanosine 5-phosphorimidazolide. In the absence of a catalytic metal ion or in the presence of Pb2+ a noticeable template effect is already observed with the dimer and the yield of long oligomers reaches a plateau with a hexamer template. Short templates give oligomers longers than the template length. The products are predominantly 2-5 linked for the Pb2+-catalyzed reaction while mixed linkages are observed in the uncatalyzed reaction.In the presence of Zn2+, a template effect is first observed with the pentamer and is maximal by the heptamer. The products are predominantly 3-5 linked. Oligomers shorter than or as long as the template are obtained in substantial yield, and longer products in much lower yields.Abbreviations G
Guanosine
- Gp
guanosine 2(3)-phosphate
- pG
guanosine 5-phosphate
- Gp!
guanosine cyclic 2,3-phosphate
- ImpG
guanosine 5-phosphorimidazolide
- ImpG*
[8-14C]-guanosine 5-phosphorimidazolide
- pGp
5-phosphoguanosine 2(3)-phosphate
- G2pG
guanylyl-[2-5]-guanosine
- G3pG
guanylyl-[3-5]-guanosine
- ImpGpG
5-phosphorimidazolide of GpG
- (pG)n (n = 2,3)
oligomers of pG
- GppG
P1, P2-diguanosine 5-diphosphate
- GppGpG
5-[guanosine 5-pyrophosphate] of GpG
- NH2pG
guanosine 5-phosphoramidate
- (pG)4+
tetramer and higher oligoguanylates with 5 terminal phosphate
- oligo(G)
oligoguanylate
- Cp
cytidine 2(3)-phosphate
- Cp!
cytidine cyclic 2,3-phosphate
- (Cp)n–1 Cp! (n= 2,3,4)
oligocytidylates terminated by 5-OH groups and 2,3-cyclic phosphates
- oligo(C)
oligocytidylate
- poly(C)
polycytidylic acid
- poly(U)
polyuridylic acid
- poly(C,G)
random copolymer of C and G
- BAP
bacterial alkaline phosphatase (E. coli)
- EDTA
ethylenediaminetetraacetic acid
- Rf
chromatographic mobility 相似文献
10.
Crude extracts or supernatants of broken cells of Clostridium formicoaceticum reduce unbranched, branched, saturated and unsaturated carboxylates at the expense of carbon monoxide to the corresponding alcohols. The presence of viologens with redox potentials varying from E
0=-295 to-650 mV decreased the rate of propionate reduction. The more the propionate reduction was diminished the more formate was formed from carbon monoxide. The lowest propionate reduction and highest formate formation was observed with methylviologen. The carbon-carbon double bond of E-2-methyl-butenoate was only hydrogenated when a viologen was present. Formate as electron donor led only in the presence of viologens to the formation of propanol from propionate. The reduction of propionate at the expense of a reduced viologen can be followed in cuvettes. With respect to propionate Michaelis Menten behavior was observed. Experiments are described which lead to the assumption that the carboxylates are reduced in a non-activated form. That would be new type of biological reduction.Non-standard abbreviations glc
Gas liquid chromatography
- HPLC
high performance liquid chromatography
- RP
reverse phase; Mediators (the figures in parenthesis of the mediators are redox potentials E
0 in mV)
- CAV2+
carbamoylmethylviologen, 1,1-carbamoyl-4,4-dipyridinium dication (E
0=-296 mV)
- BV2+
benzylviologen, 1,1-dibenzyl-4,4-dipyridinium dication (E
0=-360 mV)
- MV
methylviologen, 1,1-dimethyl-4,4-dipyridinium-dication (E
0=-444 mV)
- DMDQ2+
dimethyldiquat, 4,4-dimethyl-2,2-dipyridino-1,1-ethylendication (E
0=-514 mV)
- TMV2+
tetramethylviologen, 1,1,4,4-tetramethyl-4,4-dipyridinium dication (E
0=-550 mV)
- PDQ2+
propyldiquat, 2,2-dipyridino-1,1-propenyl dication (E
0=-550 mV)
- DMPDQ2+
dimethylpropyldiquat, 4,4-dimethyl-2,2-dipyridino-1,1-propenyl dication (E
0=-656 mV)
- PN
productivity number=mmol product (obtained by the uptake of one pair of electrons) x (biocatalyst (dry weight) kg)-1×h-1 相似文献
11.
Giesen AW Bae LC Barrett CL Chyba JA Chaykovsky MM Cheng MC Murray JH Oliver EJ Sullivan SM Brown JM Homans SW 《Journal of biomolecular NMR》2002,22(1):21-26
The implementation of [13C,13C,15N,2H] labelled amino acids into proteins allows the acquisition of high resolution triple resonance experiments. We present for the first time resonance assignments facilitated by this new labelling strategy. The absence of 1JC,C couplings enables us to measure 1JC,C scalar and 1DC,C residual dipolar coupling constants using modified HNCA experiments which do not suffer from sensitivity losses characteristic for 13C constant time experiments. 相似文献
12.
Summary 2-Amino-2-deoxyuridine reacts efficiently with nucleoside 5-phosphorimidazolides in aqueous solution. The dinucleoside monophosphate analogues were obtained in yields exceeding 80% under conditions in which little reaction occurs with the natural nucleosides.In a similar way, the 5-phosphorimidazolide of 2-amino-2-deoxyuridine undergoes self-condensation in aqueous solution to give a complex mixture of oligomers.The phosphoramidate bond in the dinucleoside monophosphate analogues is stable for several days at room temperature and pH 7. The mechanisms of their hydrolysis under acidic and alkaline conditions are described.Abbreviations A
adenosine
- C
cytidine
- G
guanosine
- U
uridine
- T
thymidine
- UN
3
2-azido-2-deoxyuridine
- UNH
2
2-amino-2-deoxyuridine
- ImpA
adenosine 5-phosphorimidazolide
- ImpU
uridine 5-phosphorimidazolide
- ImpUN
3
2-azido-2-deoxyuridine 5-phosphorimidazolide
- ImpUNH
2
2-amino-2-deoxyuridine 5-phosphorimidazolide
- pA
adenosine 5-phosphate
- pU
uridine 5-phosphate
- pUN
3
2-azido-2-deoxyuridine 5-phosphate
- pUNH
2
2-amino-2-deoxyuridine 5-phosphate
- UpA
uridylyl-[35]-adenosine
- UpU
uridylyl-[35]-uridine
- UNpA
adenylyl-[52]-2-amino-2-deoxy-uridine
- UNpU
uridylyl-[52]-2-amino-2-deoxyuridine (pUN)n n=2,3,4 [25]-linked oligomers of pUNH
2 poly(A) polyadenylic acid
- Im
imidazole
- MeIm
l-methylimidazole 相似文献
13.
Summary Two types of reactivities of thiophosphates have been demonstrated: one being nucleophilic displacement by the P-S moiety of nucleoside phosphorothioates and the other, phosphorylation via P-S cleavage as the driving force. We have designed a system where both displacement on carbon and P-S cleavage are possible. Adenosine derivatives have been synthesized with 5-deoxy-5-chloro and 5-O-tosyl substitutions as leaving groups utilizing the 3-O-phosphorothioate as the biphilic center. The main products of cyclization were 5-O-tosyl and 5-chloroadenosine 2:3-cyclic phosphate. Formation of 3:5-S-phosphorothioate was slow even using an excellent leaving group. This is possibly due to hydrogen bonding between the 2-OH and the neighboring P-O.– KOH hydrolysis of the cyclic phosphorothioate yielded 2(3) phosphorothioates in a 1:1 ratio. The 2 and 3 isomers were separated and used to study the relative rates of cyclization. The cyclization via P-S cleavage of 2(3)-O-phosphorothioates showed that the 2 isomer was more reactive. This is the first report of superior reactivity of the 3-OH of a ribonucleoside. 相似文献
14.
Summary Four E. coli Hfr strains, representing stable (Hfr Cavalli), moderately stable (AB312) and unstable (Ra-1, Ra-2) Hfr states, were used in the isolation of a series of F plasmids. Type II Fs were found to be the most prevalent F plasmid formed from all of the Hfrs, while the percentages of tra Fs increased as the stability of the Hfr increased. Two observations suggested that F formation in unstable Hfrs like Ra-2 may proceed through a type II F precursor. First, the major F products of Ra-2 are tra
+ type II Fs and, second, other F types (I, II) and classes (tra
+, tra) from Ra-2 appeared to be deletion derivatives of a larger F progenitor. By monitoring the molecular changes that occur when the Ra-2 derived type II F pWS200 is transferred from one recA host to another, we have found that all F types and classes can be generated from pWS200 in a recA-independent manner. F sequences involved in the genetic conversions of pWS200 include the oriT locus and the directly repeated junctions of F and chromosomal DNA. A model for the formation of Fs in unstable Hfrs is postulated in which a tra
+ type II F primary excision product is seen to be modified, through recA-independent processes, to other F types and classes. This model differs from the current model of F formation in that independent excision events from the Hfr chromosome are not seen as the source of type I and type II Fs.These studies have also shown that the formation of tra Fs is a recA-independent process that can occur from the F and Hfr states, that -mediated deletions in pWS200 often demonstrate regional specificity in having endpoints near the ilv operon and that genetic alterations in either replication origin of pWS200 (F oriV, chromosomal oriC) stabilize the replication of this mini-Hfr cointegrate. 相似文献
15.
Summary We have studied a number of condensation reactions involving ImpU, ImpT, ImpC, ImpA, ImpG, ImpUpG and ImpCpA as activated nucleotide donors and a variety of homo- and hetero-polynucleotides as templates. We did not obtain any evidence of a template effect with ImpU and ImpT, but observed some condensation of ImpC with GpG on appropriate templates. ImpA and ImpG take part in a number of more or less efficient template-directed reactions, as do ImpUpG and ImpCpA.Our results suggest that, on the primitive Earth, pyrimidine nucleotides could most easily have been incorporated into polymers as constituents of short oligomers, which contained one or more purine nucleotide. The linkage of the product depends strongly on the nature of the substrates; the percentage of the natural 3-5-linkage was, in some cases, less than 10% and, in others, as high as 70%. Wobble-pairing was often very effective in promoting condensations, suggesting that transition mutations would have been very frequent in prebiotic polynucleotide replication.Abbreviations and Conventions U
uridine
- T
thymidine
- C
cytidine
- A
adenosine
- G
guanosine
- pN
nucleoside-5-phosphate
- Np
a mixture of 2- and 3-phosphates of a nucleoside
- pNp
a mixture of the 2-5-diphosphate and 3-5-diphosphate of a nucleoside
- N1
2 pN2
a 2-5-linked dinucleoside monophosphate
- N1
3 pN2
a 3-5-linked dinucleoside monophosphate
- N5 ppN
a pyrophosphate derived from a nucleoside-5-phosphate. ImpN and ImpN1pN2 are 5-phosphorimidazolides of nucleosides and 3-5-linked dinucleoside monophosphates, respectively
- poly(N)
a homopolynucleotide
- poly (U1 C2 A4 G3)
a random copolymer derived from a substrate mixture containing U, C, A, G in ratio 1:2:4:3
- ODU
optical density units measured at 260 nm 相似文献
16.
Summary Crude cell-free extracts of Anabaena cylindrica synthesized adenosine-5-phosphosulphate (AP35S) and 3-phosphoadenosine-5-phosphosulphate (PAP35S) from 35SO4
2- in the presence of Mg2+, ATP and inorganic pyrophosphatase. Maximum AP35S and PAP35S were produced at pH 7.15 and 8.05, respectively. APS kinase was detected in the supernatant of crude cell-free extracts by a spectrophotometric procedure. ATP-Sulphurylase had an absolute requirement for Mg2+ and less than 30% AP35S was formed when Mg2+ was replaced by either Mn2+ or Co2+. Nucleotide triphosphates other than ATP and 2-deoxyATP were ineffective in this reaction. Maximum enzyme activity was observed at equimolar concentrations of Mg2+ and ATP and excess of either of these was inhibitory. Other nucleotide triphosphates, like GTP, UTP, CTP, TTP, ITP, or 2-deoxyATP also inhibited the enzyme activity. Inhibition by GTP was competitive with respect to ATP. ATP-sulphurylase activity was not affected by cysteine, methionine or glutathione.Abbreviations APS
adenosine-5-phosphosulphate
- PAPS
3-phosphoadenosine-5-phosphosulphate 相似文献
17.
Hisanori Suzuki Yasuharu Tanaka Daniela Tornese Buonamassa Benedetta Farina Enzo Leone 《Molecular and cellular biochemistry》1987,74(1):17-20
The effects of analogs of diadenosine 5,5-p1,p4-tetraphosphate (Ap4A) were examined on the ADP-ribosylation reaction of histone Hl catalysed by purified bovine thymus poly(ADP-ribose)transferase. Among the compounds tested, Ap4A and ApCH2PPPA were shown to be the most efficient inhibitors of the enzyme. From kinetic studies of their action, it appears that Ap4A and ApCH2pppA might be mixed type inhibitors.Abbreviations ADP-ribose
adenosine diphosphate ribose
- ADPRT
poly-(ADP-ribose)transferase
- Ap4A
diadenosine 5,5-p1,p4-tertraphosphate
-
Ap4A
diadenosine 5,5-p1,p4(-1,N6-ethenyl-)tetra-phosphate
-
ApAA
diadenosine 5,5-p1,p4(-N6(-1,N6-)bisethenyl-)tetraphosphate
- ApCH2pppA
diadenosine 5,5-p1,p4(-p1,p2-methylene-)tetraphosphate
- AppCH2ppA
diadenosine 5,5-p1,p4(-p2,p3methylene-)tetraphosphate
- AppNHppA
diadenosine 5,5-p1,p4(-p2,p3-amino-)tetraphosphate
- AppCHBrppA
diadenosine 5,5-p1,p4(-p2,p3-bromine methyno-)tetraphosphate
- CpCH2ppCH2PC
dicytidine 5,5-p1,p4(-p1,p2-p3,p4-bismethylene-)tetraphosphate
- ApCH2ppCH2pA
diadenosine 5,5-p1,p4(-p1,p2-p3,p4-bismethylene-)tetraphosphate. 相似文献
18.
Jacques Raymond Brahim Mimouni Jean-Louis Azanza 《Plant Systematics and Evolution》1994,193(1-4):69-79
The seed storage globulins from sixHelianthus and four hybrids were studied using mono and bidimensional gel SDS electrophoresis (+ 2 mercaptoethanol). The polypeptide composition of each subunit was determined. Different pairs are specifically expressed according to the species studied. Three typical patterns were discriminated. All the studied species exhibit five subunits: two of them are expressed in all the species (11 and 22). The subunit corresponding to the 11 pair is present inH. petiolaris and in the three populations ofH. annuus studied. The 2b2 pair is common toH. annuus andH. argophyllus. H. petiolaris presents two specific 2a2 and 44 pairs andH. annuus a specific 33 pair. InH. argophyllus 11 33 or 44 are never observed but are replaced by 13 and 31 pairs. Some globulins, poorly represented, are of forms but present chains of higher molecular weights (in the range 54–56 kDa). Expressing variations in the banding patterns between these species by the use of a similarity index reveals complete identity between the three populations ofH. annuus. Identity between the twoH. petiolaris studied is also observed.H. annuus andH. argophyllus appear to be closer to each other thanH. petiolaris concerning the seed storage globulins. 相似文献
19.
Hiroaki Sawai 《Journal of molecular evolution》1981,17(1):48-51
Summary Oligouridylates with more than eight chain units can serve as a template for the template-directed condensation of ImpA catalyzed by Pb2+ ion. The templates and the Pb2+ ion catalyst facilitate the formation of longer oligoadenylates with five or more units. The ratio of 3–5 linked oligomers to the 2–5 isomers increases with increasing chain length of the oligouridylate template. Short oligouridylates up to a hexamer tend to decrease the yield of oligoadenylates, and do not affect the selectivity of internucleotide linkage.Abbreviations EDTA
ethylenediaminetetracetic acid
- Tris
tris(hydroxymethyl)aminomethane
- A
adenosine
- ImpA
adenosine 5-phosphorimidazolide
- pA
adenosine 5-phosphate
- Ap
adenosine 2(3)-phosphate
- poly A
polyadenylic acid
- AppA
P1,P2-diadenosine 5-diphosphate
- pAp
adenosine 2(3),5-diphosphate
- ApA
adenylyl adenosine
- (pA)n (n = 2,3,)
oligomers of pA
- ImpApA
5-phosphorimidazolide of ApA
- U
uridine
- pU
uridine 5-phosphate
- Up
uridine 2(3)-phosphate
- poly U
polyuridylic acid
- pUp
uridine 2(3),5-diphosphate
- (pU)n (n = 2,3,)
oligomers of pU
- (pU)n – (pA)m
cooligomers composed of (pU)n and (pA)m units
- AppUpUpUpUp
pyrophosphate derived from pA and (pU)4
- AppUp
P1-(adenosine 5)-P2-(uridine 2(3)-phosphate 5) -pyrophosphate
- BAP
bacterial alkaline phosphatase
- VPD
venom phosphodiesterase
- N.P1
nuclease P1
- RNase A
pancreatic ribonuclease
- A*
radioactive adenosine 相似文献
20.
B. P. Gottikh A. A. Krayevsky M. K. Kukhanova A. A. Jatsyna A. M. Kritzyn C. L. Florentiev 《Molecular biology reports》1973,1(3):173-178
Peptide acceptor properties of phenylalanine and glycine esters of 9-(2,3-dihydroxypropyl-1)-adenine and 1-(2,3-dihydroxypropyl-1)-4-thiouracyl were investigated. All these esters appeared to be powerful inhibitors of polyphenylalanine synthesis in E. coli MRE-600 ribosomes charged with poly U. Like puromycin, esters of adenine derivatives accepted the AcPhe residue from Ac-[14C] Phe-tRNA in a ribosomal system charged with poly U. However, peptidyl esters of 9-(2,3-dihydroxypropyl-1)-adenine remained bound with ribosomes. The structure of the peptide esters synthesized was ascertained after dissociation of ribosomes into subparticles by direct comparison with the synthetic specimens.Abbreviations AcPhe
acetyl-l-phenylalanine
- HP-Ade
9-(2,3-dihydroxypropyl-1)-adenine
- Phe-HP-Ade and Gly-HP-Ade
l-phenylalanine and glycine esters of HP-Ade
- Phe-HP-TUra
l-phenylalanine ester 1-(2,3-dihydroxypropyl-1)-4-thiouracyl
- AcPhePhe-HP-Ade and AcPheGly-HP-Ade
acetyl-l-diphenylalanine and acetyl-l-phenylalanylglycine esters of HP-Ade respectively
- AcPhe-puromycin
acetyl-l-phenylalanyl-puromycin 相似文献