中国隐甲螨属2新种记述（蜱螨亚纲：甲螨亚目：耳头甲螨科）

记述隐甲螨属２新种,浙尖隐甲螨Ｄｏｌｉｃｈｅｒｅｍａｅｕｓａｔｔｅｎｕａｔｕｓｓｐ．ｎｏｖ和环纹隐甲螨Ｄｏｌｉｃｈｅｒｅｍａｅｕｓｇｙｒｏｐｌｉｃａｔｕｓｓｐ．ｎｏｖ。     

鼎湖山地区甲螨的新种和新纪录（蜱螨亚纲：甲螨亚目）

记述鼎湖山自然保护区土壤甲螨３新种,崔氏珠甲螨Ｄａｍａｅｕｓ（Ｔｅｃｔｏｄａｍａｅｕｓ）ｃｕｉｉＷａｎｇｅｔＬｕ．ｓｐ．ｎｏｖ,鼎湖赫甲螨Ｈｅｒｍａｎｎｉａ（Ｐｈｙｌｌｈｅｒｍａｎｎｉａ）ｄｉｎｇｈｕ－ｅｎｓｉｓ．ＷａｎｇｅｔＬｕ．ｓｐ．ｎｏｖ．秀琴甲螨ＬｙｒｏｐｐｉａｄｅｌｉｃａｔａＬｕｅｔＷａｎｇ．ｓｐ．ｎｏｖ并报道５个中国新纪录种,奎氏爪哇甲螨ＪａｖａｃａｒｕｓｋｕｅｈｎｅｌｔｉＢａｌｏｇｈ     

中国南方珠甲螨属种类纪要（蜱螨亚纲：甲螨亚目：珠甲螨科）

本文记述我国南方土壤甲螨珠甲螨属Ｄａｍａｅｕｓ Ｋｏｃｈ种类共９种,其中包括５新种：矩刺珠甲螨Ｄ．ｓｐｉｎｉｇｅｒ ｓｐ．ｎｏｖ．,短毛洙甲螨Ｄ．ｂｒｅｖｉｓｅｔｕｓ ｓｐ．ｎｏｖ．,姚氏珠甲螨Ｄ．ｙａｏｉ ｓｐ．ｎｏｖ．,巨突珠甲螨Ｄ．ｅｘｓｅｒｔｕｓ ｓｐ．ｎｏｖ．,鞭毛珠甲螨Ｄ．ｆｌａｇｅｌｌａｔｕｓ ｓｐ．ｎｏｖ．;１个中国新纪录盔珠甲螨Ｄ．ａｒｍａｔｕｓ（Ａｏｋｉ）;２项新组合：将ａｒｍ     

鼎湖山珠甲螨属1新种（蜱螨亚纲：甲螨亚目：珠甲螨科）

记述我国广东省鼎湖山珠甲螨属１新种,命名为叉珠甲螨,新种Ｄａｍａｅｕｓ（Ｔｅｃｔｏｄａｍａｅｕｓ）ｆｕｒｃａｔｕｓＷａｎｇｅｔＬｕ,ｓｐ．ｎｏｖ。     

中国船甲螨属记述(蜱螨亚纲:甲螨亚目:卷边甲螨科)

本文记述中国船甲螨属Ｓｃａｐｈｅｒｅｍａｅｕｓ Ｂｅｒｌｅｓｅ种类共６种,其中包括５新种：网纹船甲螨Ｓ．ｒｅｔｅｓｔｒｉａｔｕｓ ｓｐ．ｎｏｖ．,钩船甲螨Ｓ．ｕｎｃｉｎａｔｕｓ ｓｐ．ｎｏｖ．,四网船甲螨Ｓ．ｑｕａｒｉｒｅｔｉｃｕｌａｔｕｓ ｓｐ．ｎｏｖ。,双突船甲螨．Ｓｂｉｔｕｂｅｒｃｕｌａｔｕｓ ｓｐ．ｎｏｖ．,指突船甲螨Ｓ．ｄｉｇｉｔａｔｕｓ ｓｐ．ｎｏｖ和１个中国新纪录种子代船甲螨Ｓ．ｎａ     

小兴安岭地区叉肋甲螨属1新种（蜱螨亚纲：甲螨亚目：阿斯甲螨科）

记述采自黑龙江小兴安岭地区的叉肋甲螨属１新种：伊春叉肋甲螨Ｆｕｒｃｏｒｉｂｕｌａｙｉｃｈｕｎｅｎｓｉｓ,ｓｐ．ｎｏｖ。     

中国珠足甲螨属一新种及珠足甲螨属和孔珠足甲螨属的新纪录（蜱螨亚纲：甲螨亚目：珠甲螨科）

本文记述中国珠足甲螨属ＢｅｌｂａＶｏｎＨｅｙｄｅｎ的一新种角珠足甲螨Ｂ．ｃｏｒｎｕｔａｓｐ．ｎｏｖ,珠足甲螨属和孔珠足甲螨属中国６个新纪录种：南方珠足甲螨Ｂ．ａｓａｋａｗａｉＥｎａｍｉ、俄罗斯珠足甲螨Ｂ．Ｂｕｌａｎｏｖａ－Ｚａｃｈｖａｔｋｉｎａ,疣珠足甲螨Ｂ．ｖｅｒｒｕｃｏｓａＢ．－Ｚ．,塞氏珠足甲螨Ｂ．ｓｅｌｌｎｉｃｋｉＢ．－Ｚ．,棒珠足甲螨Ｂ．ｃｏｒｙｎｏｐｕｓ（Ｈｅｒｍａｎｎ）和刺孔珠足甲螨Ｐｏｒｏｂｅｌｂａｓｐｉｎｏｓａ（Ｓｅｌｌｎｉｃｋ）,并对上述部分种类作了补充描述。     

中国马珠足甲螨属一新种(蜱螨亚纲:甲螨亚目:马珠足甲螨科)

本文记述马珠足甲螨属一新种,歧马珠足甲螨Ｍａｃｈａｄｏｂｅｌｂａ ｂｉｆｕｒｃａｔａ ｓｐ．ｎｏｖ．采于云南勐仑热带植物园枯枝落叶层。模式标本保存于白求恩医科大学生物教研室。马珠足甲螨科系中国首次报道。     

植绥螨科四新种记述（蜱螨亚纲：植绥螨科）

本文记述植绥螨科４新种：长囊钝绥螨Ａｍｂｌｙｓｅｉｕｓ ｌｏｎｇｉｓａｃｃａｔｕｓ ｓｐ．ｎｏｖ．,钩囊钝绥螨Ａ．ｓｔｒｏｂｏｃｏｒｙｃｕｓ ｓｐ．ｎｏｖ．,新裴济钝绥螨Ａ．ｎｅｎｆｉｊｉｅｎｓｉｓ ｓｐ．ｎｏｖ．及侧膜盲走螨Ｔｙｐｈｌｏｄｒｏｍｕｓ ｌａｔｅｒｉｓ ｓｐ．ｎｏｖ．。     

广西瘤瘿螨属四新种记述：蜱螨亚纲：瘿螨科

４个新种是紫薇瘤瘿螨Ａｃｅｒｉａ ｌａｇｅｒｓｔｒｏｅｍｉａｅ Ｋｕａｎｇｅｔ Ｙａｎｇ,ｓｐ．ｎｏｖ．,路边青瘤瘿螨Ａｃｅｒｉａ ｇｅｉ Ｋｕａｎｇ ｅｔＬｕ,ｓｐ．ｎｏｖ．,广西瘤瘿螨Ａｃｅｒｉａ ｇｕａｎｘｉｅｎｓｅ ＫｕａｎｇｅｔＬｕ,ｓｐ．ｎｏｖ．和梧州瘤瘿螨Ａｃｅｒｉａ ｗｕｚｈｏｕｅｎｓｉｓ Ｋｕａｎｇｅｔ Ｙａｎｇ,ｓｐ．ｎｏｖ。     

Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A,G:G:A:A,and A:A:A:A tetrads

Replacement of two to four guanines by adenines in the human telomere DNA repeat dG₃(TTAG₃)₃ did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na⁺ and K⁺ solutions. Thermodynamic data showed that, in Na⁺ solution, the dG₃(TTAG₃)₃ quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K⁺ solution, the highest destabilization was caused by the 4A tetrad. In K⁺, only the unmodified dG₃(TTAG₃)₃ quadruplex rearranged into a K⁺‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.     

Oka: Behind the Barricades: Kanehsatake: 270 Years of Resistance: Spudwrench: Kahnawake Man: My Name is Kahentiiosta: Acts of Defiance

Oka: Behind the Barricades. 1998. 312 minutes. For more information contact the National Film Board of Canada, 3155 Côte de Lisse Rd., St. Laurent, Quebec H4N 2N4. Series of four videos:
Kanehsatake: 270 Years of Resistance. 1993. 120 minutes. Directed by Alanis Obomsawin .
Spudwrench: Kahnawake Man. 1997. 58 minutes. Directed by Alanis Obomsawin .
My Name is Kahentiiosta. 1995. 30 minutes. Directed by Alanis Obomsawin .
Acts of Defiance. 1992. 104 minutes. Directed by Alec MacLeod .     

Experimental demonstration of T:(G:G:G:G):T hexad and T:A:A:T tetrad alignments within a DNA quadruplex stem

A template-based approach was used to design unprecedented architectural motifs into a known DNA framework. The structure formed by the sequence d(GCGGTTGGAT) in 0.1 M Na(+) solution has been determined using molecular dynamics simulations constrained by distance and dihedral restraints derived from NMR experiments. The molecular topology has been previously observed for the sequence d(GCGGTGGAT) (Webba da Silva, M. (2003) Biochemistry 42, 14356-65). Insertion of a single thymine into the double chain reversal formed by the segment GGTGG results in the unprecedented experimental demonstration of a T:(G:G:G:G):T hexad. The bi-stranded hexad results from the pairing alignment of two G(T-G) triads. Each triad results from recognition of the sheared edge of a guanine by the Watson-Crick edge of a thymine of the segment GGTTGG. The alignment is stabilized by base-stacking of the thymine to the sugar pucker of the preceding thymine. The latter is involved in formation of the T:A:A:T tetrad alignment by forming a hydrogen bond with the free amino proton of a Watson-Crick aligned A:A mispair. We have thus established that residues in double chain reversal loops linking juxtaposed tetrads of a quadruplex stem may facilitate formation of yet unknown hydrogen bond alignments. By employing a systematic approach analysis of sequence motifs appearing in double chain reversals, bridging tetrad layers should allow for the prediction of topologies and architectural motifs appearing in biologically relevant genomic regions.     

A dimeric RNA quadruplex architecture comprised of two G:G(:A):G:G(:A) hexads,G:G:G:G tetrads and UUUU loops

Using CD and NMR, we determined the structure of an RNA oligomer, r(GGAGGUUUUGGAGG) (R14), comprising two GGAGG segments joined by a UUUU segment. A modified quadruplex structure was observed for r(GGAGGUUUUGGAGG) in solution even in the absence of K(+). An unusually stable dimeric RNA quadruplex architecture formed from two strands of r(GGAGGUUUUGGAGG) at low K(+) concentration is reported here. In each strand of r(GGAGGUUUUGGAGG), two sets of successive turns in the GGAGG segments and turns at both ends of the UUUU loops drive four G-G steps to align in a parallel manner, a core with two stacked G-tetrads being formed. Two adenine bases bind to two edges of one G:G:G:G tetrad through the sheared G:A mismatch augmenting the tetrad into a G:G(:A):G:G(:A) hexad. Thus, one molecule of r(GGAGGUUUUGGAGG) folds into a modified quadruplex comprising a G:G:G:G tetrad, a UUUU double-chain reversal loop and a G:G(:A):G:G(:A) hexad. Two such molecules further associate by stacking through the dimeric hexad-hexad interface with a rotational symmetry. The ribose rings of most nucleotides take S (close to C2'-endo) puckering, which is unusual for an RNA. K(+) can increase the stability of this quadruplex structure; the number of bound K(+) was estimated from the results of the titration experiment. Besides G:G and G:A mismatches, a network of hydrogen bonds including O4'-NH(2) and C-H..O hydrogen bonds, and the extensive base stacking contribute to the high thermodynamic stability of R14. Our results could provide the stereochemical and thermodynamic basis for elucidating the biological role of the GGAGG-containing RNA segments abundantly existing in various RNAs. Relevance to quadruplex-mediated mRNA-FMRP binding and HIV-1 genome RNA dimerization is discussed.     

Focus: Microbiome: Metagenomic Assembly: Overview,Challenges and Applications

Advances in sequencing technologies have led to the increased use of high throughput sequencing in characterizing the microbial communities associated with our bodies and our environment. Critical to the analysis of the resulting data are sequence assembly algorithms able to reconstruct genes and organisms from complex mixtures. Metagenomic assembly involves new computational challenges due to the specific characteristics of the metagenomic data. In this survey, we focus on major algorithmic approaches for genome and metagenome assembly, and discuss the new challenges and opportunities afforded by this new field. We also review several applications of metagenome assembly in addressing interesting biological problems.     

Focus: Rare Disease: Chronic Pulmonary Aspergillosis: A Brief Review

Chronic Pulmonary Aspergillosis (CPA) is a destructive pulmonary disease caused by a fungal infection, affecting mainly individuals with prior or concurrent pulmonary conditions. It has a global prevalence of 42 per 100,000 population, but in the US and Europe, prevalence is less than 1 per 100,000. The clinical definition of CPA is based on various factors accounting for comorbidities, clinical presentation, and duration. It may be categorized into five subtypes that the disease may evolve between over time. Based on global consensus covering the spectrum of low-resource to high-resource settings, diagnosis is a multi-factorial process that involves a combination of clinical presentation persisting over 3 months, radiological findings, positive culture growth, and serological tests. CPA remains underdiagnosed due to a lack of awareness and is often misdiagnosed due to the comorbidities present. Treatment options are limited due to a lack of research. Furthermore, associated comorbidities and drug interactions further complicate treatment plans. Follow-up throughout treatment should be based on understanding the predictors of mortality. Identification of potential relapse or resistance to antifungal therapy is crucial to limit the low long-term survival rate. Awareness surrounding this devastating disease needs to be raised further to enable earlier identification, improve understanding of patient factors associated with prognosis, and the future potential for targeted therapies. This review aims to raise awareness of this rare condition among practitioners, by providing an overview of common risk factors influencing the prevalence and incidence of the disease. We further discuss current approaches and recent advancements in CPA diagnosis and treatment.