四君子汤对实验性脾虚小鼠肠道菌群的影响

本文报道了四君子汤对脾虚小鼠肠道内与人类关系密切的主要菌群的影响。用大量大黄水煎液灌胃给予小鼠,造成实验性脾虚模型,引起小鼠肠道内菌群紊乱,其中双歧杆菌、乳杆菌菌量均下降,与对照组小鼠相比具有显著性差异。当小鼠服用四君子汤后,双歧杆菌,乳杆菌均上升至正常值水平。四君子汤对拟杆菌数量也有一定的影响。本研究表明,四君子汤对小鼠肠道菌群的失调具有调节功能,对脾虚小鼠的康复具有一定的促进作用。     

回春生治疗小儿腹泻临床疗效观察

回春生是双歧杆菌活菌制剂,它能直接补充肠道内的双歧杆菌数量,抑制肠道病原菌,达到恢复肠道微生态平衡的作用。我科自１９９７年１０月～１９９８年８月应用回春生治疗小儿急性腹泻病取得了明显的疗效,现报道如下：１材料与方法１１一般资料本组１８０例均为住院腹...     

低温对小鼠肠道菌群的影响及中药903Ⅱ号的调节作用

小鼠在低温环境下发生肠道菌群比例失调,应用中药903Ⅱ号能使小鼠肠道双歧杆菌、类杆菌、乳杆菌数量增加,回春生可使双岐杆菌增加,肠杆菌下降。     

扶正固本丸对小鼠肠道菌群的影响

本文报告应用扶正固本丸对小鼠肠道内与人类关系密切的主要菌群的影响。用大黄水煎液灌胃予小鼠,造成实验性脾虚模型,引起小鼠肠道内菌群紊乱,其中双歧杆菌,乳杆菌量均下降,与对照组相比有显著性差异。当服用扶正固本丸后,双歧杆菌、乳杆菌均上升至正常水平。本研究表明,扶正固本丸对小鼠肠道菌群失调具有一定的调整作用。     

双歧杆菌制品（回春生）对肝硬化患者治疗作用的观察

双歧杆菌制品（回春生）对肝硬化患者治疗作用的观察辽河油田沈阳采油厂医院１１０３１６李兆平,陆新志双歧杆菌是人体内重要的生理性有益菌。本文就回春生（双歧杆菌活菌制品）改善肝硬化患者临床有关症状的疗效进行了初步观察,其结果报告如下。１临床资料选择肝硬化患...     

肠道菌群调整对肝硬化病人临床症状及实验室指标的观察

目的研究肝硬化与肠道菌群的关系。方法应用常规保肝治疗和加用回春胶囊两组共６８例进行观察。结果治疗组与对照组在临床症状、生化指标及肠道菌群方面相比较均有明显改善。结论双歧杆菌活菌制剂有利于维护肝硬化患者的肠道微生态平衡,减少内毒素的产生、吸收,使病情稳定并向好的趋势转化。     

回春生胶囊治疗肠易激综合征菌群失调的研究

本文报道应用回春生胶囊（丽珠肠乐）治疗肠易激综合征（ＩＢＳ）和对菌群失调疗效研究结果。６４例病人,随机分成两组,治疗组３５例,回春生胶囊每次１亿活菌,一日２次,连续服用１个月：回春生胶囊治疗后的积分为治疗前的４０．７％（３７／９１）,较对照组有显著差异（Ｐ＜０．０５）。另外,全部病人还进行了粪便的９种常见厌氧菌和需氧菌的定量研究。结果发现治疗后双歧杆菌、类杆菌和肠杆菌的数值呈明显增加,说明回春生胶囊是通过补充双歧杆菌的数量,纠正肠菌群失调,对ＩＲＳ有良好的治疗作用。     

双歧杆菌活菌制剂治疗腹泻的临床疗效观察

腹泻是临床上常见症状,过去常规用抗生素及止泻药治疗效果不佳,现认为肠道菌群失调是腹泻的主要原因,为进一步证实这一理论,我们用回春生胶囊即双歧杆菌活菌制剂治疗腹泻３２例,用多酶片为对照组治疗２０例,效果满意,现报道如下：１材料与方法１１病例选择５２例...     

双歧杆菌合剂促进急性坏死性胰腺炎肠粘膜损伤修复的实验研究

观察双歧杆菌合剂对急性坏死性胰腺炎（ＡＮＰ）犬肠粘膜损伤修复的作用。经主胰管注入牛磺胆酸钠和胰蛋白酶复制犬ＡＮＰ模型,观察ＡＮＰ时及双歧杆菌合剂治疗后肠粘膜组织结构变化,肠组织蛋白、丙二醛（ＭＤＡ）含量及肠通透性改变,检测血中内毒素（ＬＰＳ）水平,脏器细菌培养。结果发现,双歧杆菌合剂治疗后,ＡＮＰ犬肠粘膜损害明显减轻,肠粘膜绒毛宽度、高度和面积显著增加,肠组织蛋白含量增加,肠通透性显著下降,血中ＬＰＳ水平下降１～２倍,脏器细菌易位率减少３７５％。结论：双歧杆菌合剂能显著促进ＡＮＰ时肠粘膜损伤的修复,保护肠屏障功能,减少肠道ＬＰＳ和细菌易位     

中药人参合剂对提高小鼠定植抗力作用的初步研究

本实验用肠炎杆菌攻击正常小白鼠,肠炎杆菌菌液浓度为１０８个／ｍｌ,灌喂０．２ｍｌ,造成肠炎杆菌在肠道内大量定植后,分别用中药人参合剂及复方新诺明（ＣＯ－ＳＭＺ）治疗,连续６天后,观察对比两种药物对受攻击小鼠的治病效果。检测小鼠粪便中的四种菌群成员,肠炎杆菌、肠杆菌、双歧杆菌和乳杆菌。结果表明：中药人参合剂治疗组肠炎杆菌显著下降,与ＣＯ－ＳＭＺ组相似（Ｐ＞０．０５）,但双歧杆菌与乳杆菌显著升高,与复方新诺明组相比有显著性差异（Ｐ＜０．０５）。我们认为中药人参合剂治疗肠道感染的主要机制是中药人参合剂扶植正常菌群生长,提高小鼠的定植抗力的作用。     

对回春生生产菌种DM8504菌株的研究总结报告

本文对回春生生产菌种双歧杆菌ＤＭ８５０４株的研究作了总结报道。ＤＭ８５０４林是青春型双歧杆菌的一个变种,该菌株具有明显的生态效应,对调整肠道菌群失调有明显的效果。该菌淋经过试管内、动物体内及人体内试验证明是一个理想的微生态调节剂生产菌株。通过实验和临床观察证明,该菌株对降低血内毒素,治疗肝炎等多种疾病均有明显的辅助治疗作用。该菌株是从健康人的大便标本经分离培养并加以驯化等复杂过程而获得的生产菌株。     

DIFFERENTIAL LOCALIZATION OF CELL SURFACE AND SECRETORY COMPONENTS IN RAT INTESTINAL EPITHELIUM BY USE OF LECTINS

Sections through various levels of small intestine from adult male rats were examined by fluorescence microscopy after treatment with fluorescein isothiocyanate-labeled lectins from Dolichos biflorus, Lotus tetragonolobus, Ricinus communis, and Triticum vulgare (wheat germ). The latter three lectins reacted with the microvillar portion of the epithelial cells lining the crypts and villi in sections of intestine adjacent to the pylorus. This pattern of reactivity was sharply altered along the first 15 cm of intestine so that in sections distal to this point the luminal surfaces of only those epithelial cells in the crypts and at the base of the villi reacted with the L. tetragonolobus and R. communis lectins, whereas the wheat germ lectin reacted with the surfaces of the cells lining the villi. In sections from the distal end of the small intestine, all three lectins reacted with the surfaces of cells only at the base of the villi and in the crypts. These results show a difference in surface components in cells at various portions on the villi and the dependence of these differences on the region of intestine. The D. biflorus lectin reacted with approximately 25% of the goblet cells at each level of intestine studied whereas the reactivities of the goblet cells with the other three lectins were dependent upon the region of intestine.     

CHANGES IN INTESTINAL CELL KINETICS IN THE SMALL INTESTINE OF LACTATING MICE

The enlargement of the small intestine of mice during lactation is due, at least in part, to hyperplasia in the mucosal crypts and villi. The number of cells per crypt increases by 130% and the cell production rate by 63% after 15 days of lactation. These parameters were measured from crypt squashes and sections using both double-label and PLM techniques. Neither the numbers of crypts and villi in the small intestine nor the turnover time of post-mitotic cells on the villi changed. A number of factors appear to act in concert during lactation to trigger this increase in epithelial cell number in the small intestine. The experiments reported suggest a role for the increased quantity of food consumed by the lactating animal, for changing hormonal levels, and for the increased demands placed on the body by milk production.     

FISSION OF CRYPTS IN THE SMALL INTESTINE OF THE IRRADIATED MOUSE

Following 1600 R of X-rays there was a reduction in the number of crypts in the small intestine of the mouse by 77% as measured 5 days later. However, by 21 days the number had increased steadily to levels approaching normal. The number of villi did not change following irradiation. The mechanism of increase in crypt number was by budding and fission of repopulated crypts which had become larger than normal. Some aspects of crypt organization are discussed.     

THE ROLE OF ANTISENSE OLIGONUCLEOTIDES IN THE WAVE OF GENOMIC INFORMATION

Technologies which efficiently dissect gene function and validate therapeutic targets are of great value in the post-sequencing era of the human genome project. The antisense oligonucleotide approach can directly use genomic sequence information, in a relatively time and cost effective manner, to define a gene's function and/or validate it as a potential therapeutic target. Antisense oligonucleotide inhibitors of gene expression may be applied to cellular assays (in vitro) or animal models of disease (in vivo). Information generated by this approach may then direct or supplement traditional drug discovery programs, or support development of the antisense oligonucleotide inhibitor, used to validate the target, as a drug.     

POPULATION DYNAMICS OF INTESTINAL EPITHELIA IN THE RAT TWO MONTHS AFTER PARTIAL RESECTION OF THE ILEUM

Sprague-Dawley rats that had been subjected 2 months previously to partial resection (10 per cent) of the small intestine and an equal number of control rats were injected with tritiated thymidine and sacrificed at intervals during the subsequent 16 hours. Segments of duodenum, jejunum and ileum were prestained by the Feulgen technique and radioautographed. The proportion of crypt cells bearing labeled nuclei, the percentage of labeled crypt cells in mitosis and the appearance of labeled crypt cells on the villi were determined. Comparison of control and resected rats showed that (a) the proportion of intestinal crypt cells incorporating thymidine was considerably greater and uniformly high throughout the shortened intestine, (b) the life cycle of crypt cells was slightly reduced, and was uniform throughout the shortened intestine, and (c) the time during which cells were retained in crypts was markedly reduced. On the basis of persistent, generalized increase in the production of crypt cells, and on prior evidence that the epithelial cells of shortened intestine continue to have a brief life span and evidence of metabolic immaturity, the existence of a humoral factor, tentatively called "intestinal epithelial growth hormone," is postulated.     

金银花水提物对肠道微生态失调大鼠的调整作用

目的研究金银花水提取物作为微生态调节剂对肠道微生态失调大鼠肠道菌群的调整作用。方法结扎大鼠胆总管造成肠道菌群失调模型后分别以金银花、丽珠肠乐、金银花与丽珠肠乐合剂、生理盐水灌胃,于灌胃4d后测定各组大鼠肠道菌群组分、乙酸含量及肝脏中肠杆菌易位情况。结果大鼠肠道菌群失调得到恢复,肠道内乙酸含量增加,易位至肝脏的肠杆菌数量减少,与自然恢复组相比,差异有统计学意义（P〈0.05）,金银花与丽珠肠乐合剂组效果最佳。结论金银花水提物作为益生元对大鼠肠道菌群失调具有调整作用。     

胶类物质对固体制剂中青春双歧杆菌稳定性研究*

考察了５０Ｌ发酵罐中青春双歧杆菌在混合胶类物质的果蔬汁底料中的生长及其溶氧率,ｐＨ值、可溶性固形物等变化规律,就培养后的双歧杆菌制备活菌固体制剂的工艺方法进行比较。结果表明胶类物质能缓冲培养基质中酸性环境,减少发酵和制剂过程中空气氧对菌细胞的毒害,干燥后的固体制剂中活菌回收率高,贮存的稳定性增加。提示了明胶和黄原胶在配料干燥后对菌细胞的包埋及保护作用。并制定出关于青春双歧杆菌保健片或粉料的简便生产     

微生态制剂“培菲康”治疗小儿感染性腹泻及便秘100例临床研究

培菲康为双歧杆菌、嗜酸乳杆菌、粪链球菌组成的人体肠道微生态活菌制剂,本文采用此制剂治疗小儿菌痢２２例,有效率８１．８２％;小儿肠炎４７例,有效率８６．６％;便秘３１例,有效率达８７．１％。全部１００例,总有效率为８６％。所有病例未用抗菌药物。本文对微生态药物培菲康的药理作用及特点进行了讨论。     

微囊藻毒素-LR对小鼠肠道消化酶的影响

微囊藻毒素-LR（MCLR）是一类对动物和人类健康影响很大的蓝藻毒素。已有调查认为MCLR能导致某些胃肠道疾病,相关实验室资料也证实MCLR能在肠道积累并引起肠道损伤。研究对小鼠连续腹腔染毒MCLR 28d,观察肠道病理水平及超微结构的变化,并测定肠黏膜刷状缘膜酶活性。结果显示,小肠绒毛受损较严重,绒毛数量减少、部分脱落至肠腔,固有层及黏膜下层水肿、充血;电镜观察发现肠细胞细胞质电子密度降低,伴有线粒体肿胀、细胞核变形现象;肠黏膜二糖酶（蔗糖酶、麦芽糖酶、乳糖酶）、碱性磷酸酶及-谷氨酰转移酶活性均呈下降趋势。在亚慢性MCLR染毒条件下,肠道的消化功能可能受到抑制,进而导致机体对营养物质吸收不良。研究对理解蓝藻毒素引起的胃肠道不适（如恶心、呕吐、腹痛、腹泻）提供了新的证据。