羊踯躅的组织培养与快速繁殖

1植物名称 羊踯躅（Rhododendron molleG．Don）。 2材料类别当年生茎尖或茎段。     

白花花楸的组织培养和快速繁殖

1植物名称 白花花楸[Sorbusaria（L．）Crantz．]。 2材料类别当年生种子。     

山桐子的组织培养和快速繁殖

1植物名称山桐子（Idesia polycarpa Maxim）。 2材料类别当年生枝条的腋芽或茎尖新萌顶芽。 3培养条件芽萌动启动培养基：（1）改良的MS（除钙盐外,其余大量元素用MS的2／3量,以下同此）＋TDZ0．02mg．L^-1（单位下同）＋6．BA1．0＋NAA0．05;（2）改良的MS＋TDZ0．02＋6．BA1．5＋NAA0．05。     

大叶落地生根的组织培养和植株再生

1植物名称大叶落地生根(Kalanchoe daigremon-tiana Hamet.et Perr.)。2材料类别当年生枝条上的腋芽。3培养条件(1)芽萌动培养基:MS 6-BA0.5mg·L-1(单位下同) NAA0.05;(2)分化培养基     

扭肚藤的组织培养和快速繁殖

1植物名称扭肚藤（Jasminum amplexicule Buch）,又名白花茶、假素馨、青藤仔花、左扭藤等。 2材料类别当年生幼嫩茎段。     

管花蒲公英的组织培养

1植物名称管花蒲公英(Neo-Taraxacum siphonanthum). 2材料类别种子繁殖后长出幼苗,取其叶片和叶脉.     

岩芋的组织培养

1植物名称岩芋(Remusatia vivipara Schott). 2材料类别珠芽. 3培养条件(1)丛芽诱导培养基:MS 6-BA 4 mg·L-1(单位下同) 2iP 4 NAA 0.5;(2)丛芽增殖培养基:MS 6-BA 4;(3)生根培养基:MS NAA 0.5.培养基中蔗糖浓度为3%,琼脂固化,pH 5.8.培养温度(27±2)℃,光照时间12 h·d-1 ,光照度2000 1x.     

龙葵叶的组织培养

1植物名称龙葵(Solanum nigrum L.). 2材料类别幼叶.     

金缕梅的组织培养

1植物名称金缕梅(Hamamelis mollis O1iv.). 2材料类别浙江龙王山自然保护区采收的当年健康成熟的种子. 3培养条件种子萌发培养基:(1)MS0;诱导培养基:(2)MS 6-BA 1.0 mg·L-1 (单位下同) NAA 0.1 2,4-D 0.1;增殖培养基:(3)MS 6-BA 0.2 NAA0.1,(4)MS 6-BA 1.0 NAA 0.1;壮苗培养基:(1)MS0;生根培养:(5)1/2MS KT 0.1 NAA 0.6,(6)1/2MS 6-BA 0.1 NAA 1.0.培养基(1)～(4)附加蔗糖30 g·L-1,(5)和(6)附加蔗糖20 g·L-1 ,琼脂为6.5 g·L-1 ,pH 5.8.培养温度(25±1)℃,种子萌发培养和生根培养的前期进行暗培养,其余阶段均照光,光照时间12 h·d-1 ,光照度为2 500～3 000 1x.     

广东石斛的组织培养

1植物名称广东石斛(Dendrobium wilsonii Rolfe). 2材料类别幼芽.     

Triterpenoidal saponins from Hydrocotyle sibthorpioides

Oleanane-type triterpenoidal saponins, hydrocosisaponins A-F (1-6), along with a known saponin, hydrocotyloside VII (7), were isolated from Hydrocotyle sibthorpioides. Their structures were established on the basis of spectroscopic analyses including NMR spectroscopic techniques ((13)C, (1)H, COSY, HMQC, HMBC, TOCSY and NOESY). Biological evaluation established that saponins possessing four sugar units (three d-glucoses and one l-arabinose) (4-7) exhibited moderate cytotoxicity against KB, Daoy and WiDr human tumor cell lines.     

Triterpenoid saponins from Hydrocotyle bonariensis Lam

Phytochemical investigation of the under-ground parts of Hydrocotyle bonariensis led to the isolation of five oleanane-type triterpenoid saponins, 3-O-{β-D-glucopyranosyl-(1 → 2)-[α-L-arabinopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl}-21-O-(2-methylbutyroyl)-22-O-acetyl-R(1)-barrigenol, 3-O-{β-D-glucopyranosyl-(1 → 2)-[α-L-arabinopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl}-21-O-(2-methylbutyroyl)-28-O-acetyl-R(1)-barrigenol, 3-O-{β-D-glucopyranosyl-(1 → 2)-[α-L-arabinopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl}-21-O-acetyl-R(1)-barrigenol, 3-O-{β-D-glucopyranosyl-(1 → 2)-[α-L-arabinopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl}-R(1)-barrigenol, and 3-O-{β-D-glucopyranosyl-(1 → 2)-[α-L-arabinopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl}-22-O-(2-methylbutyroyl)-A(1)-barrigenol, together with the known saniculoside-R1. Their structures were established by 2D NMR techniques and mass spectrometry. Six compounds were evaluated against two human colon cancer cell lines, HCT 116 and HT-29. Two compounds showed weak cytotoxicity with IC(50) 24.1 and 24.0, 83.0 and 83.6 μM against HT-29 and HCT 116, respectively.     

Quercetin 3-(6″-caffeoylgalactoside) from Hydrocotyle sibthorpioides

In addition to quercetin, quercetin 3-galactoside and isorhamnetin, a new caffeoylgalactoside has been isolated from Hydrocotyle sibthorpioides and identified by chemical and spectral data as quercetin 3-O-β-d-(6″-caffeoylgalactoside).     

菠萝蜜的组织培养和植株再生

1植物名称菠萝蜜（Artocarpus heterophyllus Lam．）,又称树菠萝、木菠萝。 2材料类别顶芽和腋芽。 3培养条件（1）外植体接种培养基：MS＋6-BA1．5mg．L^-1（单位下同）＋KT0．5＋30g.L^-1蔗糖;（2）启动培养基：MS＋6-BA2．0＋KT1．0＋GA30．5＋20g．L^-1蔗糖;（3）增殖培养基：MS＋6-BA1．5＋KT0．1＋GA30．5＋40g．L^-1蔗糖;     

辣木的组织培养与快速繁殖

1植物名称辣木(Moringa oleifera Lam.)。2材料类别3~7d实生籽苗。3培养条件以MS为基本培养基。(1)实生籽萌发诱导培养基:MS NAA0.5mg·L-1(     

西伯利亚杏的组织培养

1植物名称 西伯利亚杏[Armeniaca sibirica（Linn．）Lam．],别名山杏。 2材料类别 休眠枝条。 3培养条件 基本培养基为MS。（1）诱导培养基：MS＋6-BA1．0mg·L^-1（单位下同）＋IBA0．2;（2）增殖培养基：MS＋6-BA0．8＋IBA0．2;（3）生根培养基：MS＋IBA0．4。     

百合竹的组织培养与快速繁殖

1植物名称百合竹（Dracaena reflexa Lam.）。2材料类别带节茎段。3培养条件初代培养和继代增殖培养基：（1）MS＋6-BA5.0mg·L-1（单位下同）＋NAA0.4;（2）MS＋6-BA2.0＋NAA0.2;（3）MS＋6-BA1.0＋NAA0.1;（4）MS＋6-BA0.5＋NAA0.01。生根培养基：     

Light effects on leaf development and photosynthetic capacity of Hydrocotyle bonariensis Lam.

Rates of net CO₂ uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m^-2 day^-1 PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m^-2 day^-1). Maximum net CO₂ uptake rates were reached after 5 to 6 days expansion for both the low and the high PAR leaves. Leaves grown at high PAR had higher maximum photosynthetic rates and a higher PAR required for light saturation but showed a more rapid decline in rate with age than did low PAR leaves. To assess the basis for the difference observed in photosynthetic rates, CO₂ diffusion conductances and the mesophyll surface available for CO₂ absorption were examined for mature leaves. Stomatal conductance was the largest conductance in all treatments and did not vary appreciably with growth PAR. Mesophyll conductance progressively increased with growth PAR (up to 48 mol m^-2 day^-1) as did the mesophyll surface area per unit leaf area, but the cellular conductance exhibited most of its increase at low PAR (up to 4.8 mol m^-2 day^-1).     

角蒿的离体培养与快速繁殖

1植物名称角蒿（Incarvillea sinensis Lam.）。 2材料类别种子萌发的无菌苗下胚轴和子叶。 3培养条件基本培养基为MS。（1）种子萌发培养基：1/2MS;（2）丛生芽诱导培养基：MS＋6-BA2mg·L-1（单位下同）＋NAA0.2;（3）丛生芽增殖培养基：MS＋     

三叶悬钩子的组织培养

1植物名称三叶悬钩子(Rubus delavayi Franch.). 2材料类别腋芽. 3培养条件基本培养基为MS.启动培养基(即初代培养基):(1)MS培养基;继代培养基:(2)MS 6-BA 0.2 mg·L-1(单位下同) NAA 0.1;生根培养基:(3)1/2MS IBA 1.5.以上培养基均附加30%蔗糖和6 g·L-1琼脂,pH 5.8.培养温度为(23±2)℃,光照时间12 h·d-1,光照强度40～50μmol·m-2·s-1.