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1.
The Development of Desiccation-tolerance and Maximum Seed Quality During Seed Maturation in Six Grain Legumes 总被引:3,自引:0,他引:3
Physiological maturity, i.e. the time when seedsreach their maximum dry weight during development, occurredwhen maturation drying on the parent plant in the field hadreduced seed moisture content to approximately 60 per cent infaba bean (Vicia faba L.), lentil (Lens culinaris Medic.), chickpea(Cicer arietinum L.), white lupin (Lupinus albus L.), soya bean(Glycine max [L.] Merr.) and pea (Pisum sativum L.) The onsetof desiccation-tolerance, i.e. the ability of seeds to germinatefollowing harvest and rapid artificial drying, coincided withphysiological maturity, except in pea where it occurred a littleearlier at about 70 per cent moisture content. Maximum seedquality as determined by maximum viability, minimum seedlingabnormalities and maximum seedling size occurred in pea, chickpeaand lupin when seeds were harvested for rapid drying at physiologicalmaturity; but for maximum seed quality in the other speciesmaturation drying had to proceed further - to about 45 per centmoisture content in soya bean and to about 30 per cent moisturecontent in lentil and faba bean seed crops. Much of this variationamongst the six species, however, was due to differences inthe variation in maturity within each seed crop. Results forindividual pods showed that peak maturity, i.e. maximum seedquality following harvest and rapid artificial drying, was achievedin all six species once maturation drying had reduced the moisturecontent of the seeds to 4550 per cent. In pea, faba beanand soya bean there was a substantial decline in viability andan increase in seedling abnormalities when harvest was delayedbeyond the optimal moisture content for harvest. 相似文献
2.
Variation in fruit-set, cone crop, seed viability and seedlingvigour ofAbies pinsapoBoiss. were recorded at two differentstand densities during four consecutive years (19901993).In this period only two cone crops were observed (1991 and 1992).Extreme differences were found in response to density. In thelow density stand trees had a greater seed-cone production andfruit-set in each of the two crop years. Also, significant differenceswere recorded in the seed crop viability: in the low densityzone average seed viability was only 21% whereas in the highdensity one it was 82%. Other important differences such ascone size, number of seeds per cone and seedling vigour wererecorded at different densities. The results suggest that bothpollen availability and pollen origin in low density standsinfluence the frequency and quality of filled seeds. The resultshave practical implications for the management ofA. pinsapoforseed production and subsequent reforestation. Abies pinsapo; inbreeding; mating system; plant density; cone crop; seed viability; seedling vigour; Mediterranean fir 相似文献
3.
Effect of High Temperature Stress at Anthesis on Grain Yield and Biomass of Field-grown Crops of Wheat 总被引:5,自引:0,他引:5
Spring wheat (Triticum aestivumL., Chablis) wasgrown under field conditions from sowing until harvest maturity,except for a 12-d period [7082 days after sowing (DAS)coinciding with anthesis] during which replicated crop areaswere exposed to a range of temperatures within two pairs ofpolyethylene-covered temperature gradient tunnels. At 82 DAS,an increase in mean temperature from 16 to 25 °C duringthis treatment period had no effect on above-ground biomass,but increased ear dry weight from 223 to 327 g m-2and, at 83DAS, reduced root biomass from 141 to 63 g m-2. Mean temperatureover the treatment period had no effect on either above-groundbiomass or grain yield at maturity. However, the number of grainsper ear at maturity declined with increasing maximum temperaturerecorded over the mid-anthesis period (7679 DAS) and,more significantly, with maximum temperature 1 d after 50% anthesis(78 DAS). Grain yield and harvest index also declined sharplywith maximum temperature at 78 DAS. Grain yield declined by350 g m-2at harvest maturity with a 10 °C increase in maximumtemperature at 78 DAS and was related to a 40% reduction inthe number of grains per ear. Grain yield was also negativelyrelated to thermal time accumulated above a base temperatureof 31 °C (over 8 d of the treatment from 5 d before to 2d after 50% anthesis). Thus, grain fertilization and grain setwas most sensitive to the maximum temperature at mid-anthesis.These results confirm that wheat yields would be reduced considerablyif, as modellers suggest, high temperature extremes become morefrequent as a result of increased variability in temperatureassociated with climate change.Copyright 1998 Annals of BotanyCompany Triticum aestivum, spring wheat, temperature, grain number, grain yield, root growth. 相似文献
4.
Changes in Levels and Integrity of Ribosomal RNA During Seed Maturation and Germination in Carrot (Daucus carota L.) 总被引:1,自引:0,他引:1
THOMPSON SHEILA; BRYANT J. A.; BROCKLEHURST P. A. 《Journal of experimental botany》1987,38(8):1343-1350
Thompson, S., Bryant, J. A. and Brocklehurst, P. A. 1987. Changesin levels and integrity of ribosomal RNA during seed maturationand germination in carrot (Daucus carota L.).J. exp.Bot. 38: 13431350. Amounts and integrity (percentage of complete un-nicked molecules)of ribosomal RNA (rRNA) during germination in carrot seed lotsof differing vigour and viability were examined using aqueousand formamide gel electrophoresis. In unaged seed, amounts ofrRNA per seed in dry seed are not related to maturity, vigouror viability. However, rRNA in immature seed is more vulnerableto damage by ageing than rRNA in mature seed; thus, amountsof rRNA per seed in aged seed are indicators of vigour and viability.On imbibition of aged or unaged seed, more rRNA fragmentationoccurs in immature seed than in mature seed, and the time atwhich the effects of synthesis outweigh the effects of degradationis earlier during germination in mature than in immature seed. Key words: rRNA, seed maturation, seed germination, Daucus carota L. 相似文献
5.
Steinbach Hayde S.; Benech-Arnold R.L.; Kristof Graciela; Sanchez R.A.; Marcucci-Poltri Susana 《Journal of experimental botany》1995,46(6):701-709
Preharvest sprouting is a major problem in Sorghum cropswhich leads to losses in seed viability and produces importantdecreases in grain weight. In this paper we aimed to have aninsight into the physiological basis of pre-harvest sproutingresistance in this crop by assessing germinability, ABA embryoniccontent and embryonic sensitivity to ABA during seed developmentin three varieties presenting contrasting sprouting behaviour:Redland B2 (very susceptible), SC 650 (moderately resistant)and IS 9530 (very resistant). Redland B2 caryopses were ableto germinate with high germination indices from early stagesof development, while caryopses from IS 9530 did not presentgermination indices different from 0 until near physiologicalmaturity. SC 650 (moderately resistant) grains presented anintermediate pattern of behaviour. In all three varieties isolatedembryos were able to germinate with maximum germination indicesfrom as early as 15 d after pollination (DAP). Differences ingrain dormancy level were not paralleled by a consistently differentendogenous ABA content throughout maturation. However, whenABA embryonic content was measured in incubated 35 DAP caryopses,ABA level in B2 embryos after 24 h of incubation was found tobe less than half that observed in IS 9530 embryo after thesame period of incubation. In addition, B2 embryos were foundto be 10fold less sensitive to the inhibitory effectof ABA than embryos from the other two varieties. These resultsexplain to a considerable extent differences in germinabilitybetween sproutingresistant and susceptible varietiesand are consistent with differences in sprouting behaviour. Key words: Sorghum bicolor, abscisic acid, pre-harvest sprouting, seed development, germination 相似文献
6.
Summary Responses of pollen grains of Nicotiana tabacum to high humidity (95% RH, 4 h) and temperature (38°/45° C, 4 h) stresses were investigated. Pollen grains were subjected to only RH or only temperature, or to both of these stresses. Their viability was assessed on the basis of the fluorochromatic reaction (FCR) test, and vigour was assessed on the basis of the time taken for in vitro germination as well as on the emergence of pollen tubes through the cut end of semi-vivo implanted styles. None of the stress conditions affected pollen viability and high RH or high temperature stress did not individually affect pollen vigour. However, pollen vigour was markedly affected when both the stresses were given together. Pollen grains subjected to high RH at 38° C took a longer time to germinate in vitro and the pollen tubes emerged later from the cut end of the semi-vivo styles; division of the generative cell was also delayed. Pollen grains subjected to high RH at 45° C failed to germinate in vitro, but did germinate on the stigma. Many pollen tubes subjected to this treatment showed abnormalities, and the growth of pollen tubes in the pistil was much slower than that observed in other treatments. Pollen samples subjected to all of the stress conditions were able to induce fruit and seed set. The implications of these results on the relationship between the FCR test and viability, and between viability and vigour, especially in stressed pollen, are discussed. 相似文献
7.
Seeds of different vigour also differ in their germination ability.In rice (Oryza sativa), this difference was correlated withthe level of incorporation of 35S-methionine into 25-60% ammoniumsulphate precipitable material that was rich in amylase proteins.This protein fraction, from dry seeds, contained no -amylaseactivity. In contrast, ß-amylase activity was presentin all seed stocks capable of 99% germination, although thelevel was lower in seeds that grew slowly when germinated. Inlow viability low vigour stock (i.e. extensively deterioratedseeds) ß-amylase activity was absent. Alpha-amylaseactivity in all stocks was detected only after 24 h from thestart of imbibition. These results indicate that ß-amylaseactivity is reliable indicator of the germination ability ofrice seed stocks and of their vigour during germination.Copyright1995, 1999 Academic Press Rice (Oryza sativa L.,), germination, ß-amylase, -amylase, seed vigour 相似文献
8.
Seedling emergence and subsequent growth from untreated seeds and selected-germinated seeds which had been dried before sowing (low-moisture-content germinated (LMCG) seeds) were compared in cellular trays in a glasshouse and in the field following transplanting. Three seed lots of different quality of the same cultivar were used for cabbage and cauliflower. In all cases, seedling emergence was greater, earlier and more uniform from LMCG seed than that from untreated seeds. LMCG seed effectively eliminated the differences due to seed quality shown by untreated seeds at emergence suggesting an improvement in seed vigour as well as viability from the selection treatment. Higher quality seed and LMCG cauliflower seed led to larger curd weights and therefore an increase in marketable yield at harvest compared to that from lower quality seeds. However, differences in transplant uniformity due to seed quality and seed treatment had little impact of agronomic significance following transplanting. 相似文献
9.
COALDRAKE PETER D.; PEARSON CRAIG J.; SAFFIGNA P. G. 《Journal of experimental botany》1987,38(4):558-566
Coaldrake, P. D., Pearson, C. J. and Saffigna, P. G. 1987. Grainyield of Pennisetum americanum adjusts to nitrogen supply bychanging rates of grain filling and root uptake of nitrogen.J.exp. Bot 38: 558566. Pearl millet (Pennisetum americanum(L.)Leeke) was grown in containers at three constant rates of nitrogensupply or with the nitrogen supply increased from the lowestto the highest rate during panicle differentiation or at anthesis.We measured the rate and duration of nitrogen and dry weightgain by individual grains and nitrogen (15N) uptake by rootsand its distribution during grain filling. The total amountsof nitrogen and dry weight in all grain per plant at the lowestnitrogen supply were 8% and 14% respectively of plants growncontinuously at the highest rate of nitrogen. This was becauselow rates of nitrogen supply reduced grain number, mean grainweight and the nitrogen content of each individual grain. Theamino acid composition of the grain protein was affected onlyslightly by nitrogen treatments. Rates of grain growth were sensitive to nitrogen supply whereasthe duration of nitrogen movement to the grain was not. Nitrogenuptake by roots continued throughout grain filling; rates ofuptake per g root in plants given least nitrogen were one-halfthose of plants given the highest amount of nitrogen. A changefrom lowest to highest nitrogen supply at panicle differentiationincreased the uptake of nitrogen by roots and the rates of growthof individual grains, to the rates observed in plants whichhad been supplied continuously with the highest nitrogen. Whenthe change in supply was made at anthesis there was rapid movementof nitrogen into the plant but this was not translated intomore rapid grain growth. Key words: Nitrogen supply, Pennisetum americanum, grain yield, root uptake 相似文献
10.
Mitochondrial ADP/O and RC ratios have been determined in two-day-oldshoots of nine wheat (Triticum aestivum) varieties of knownaverage grain yield. Both ratios were highly correlated withaverage grain yield. ADP/O and RC ratios of germinating shootswere also correlated with yield within a variety when yieldwas varied by irrigation and different nitrogen fertilizer treatments.It is proposed that the observed correlations are the resultof mitochondrial ADP/O and RC ratios being partly controlledduring germination directly or indirectly by seed-based metabolismwhich reflects the metabolic activity and total carbohydratedeposited in seed during seed formation. 相似文献
11.
The morphology of flowering of several cultivars of Dioscorearotundata, Poir is described. Unisexuality and dioecism occurcommonly although complete flowers have been observed occasionally.The pollen grains are sticky and cannot be dispersed by wind;small insects are probably the main agents of pollination. Pollengrain viability appears to be low. Fruit and seed set are alsovery low and usually not more than five viable seeds are producedon one plant. Production of hybrid plants is feasible if filledseeds are selected and germinated following the end of seeddormancy. Seedling development is described from seed germinationto the establishment of several leaves on the seedling. 相似文献
12.
Evaluation of Seed Storage Conditions and Genetic Diversity of Four Crucifers Endemic to Spain 总被引:1,自引:1,他引:0
Traditional assays (germination and vigour), as well as biochemicalassays using isozyme loci, were performed to evaluate the effectof different seed storage conditions in Coincya rupestris, Iberispectinata, Moricandia moricandiodes and Vella pseudocytisus.Seeds had been stored for 2430 years under long-term(LT) and short-term (ST) conditions. Fresh seeds of the samespecies from natural populations (NP) were also collected in1996 and used for comparison. Isozyme loci were used as markersof genetic deterioration and to determine the genetic diversityof samples. The germinability, vigour and enzyme activity ofLT samples were comparable to values obtained using freshlycollected NP samples, showing the effectiveness of the long-termstorage conditions. Copyright 1999 Annals of Botany Company Endemics, genetic diversity, germination, isozymes, seed ageing, seed storage, vigour. 相似文献
13.
Previous studies have demonstrated that pollen of sorghum [Sorghumbicolor (L.) Moench] loses capacity to both germinate in vitroand to set seed in vivo soon after being shed. The current studyevaluates the capacity for dehydrated pollen to effect in vitrogermination, reduce tetrazolium chloride, and set seed on cytoplasmicmale sterile plants. Morphological changes during pollen germinationwere examined by scanning electron microscopy (SEM). Close to70% of the pollen germinated in 5 min, or less, when collectedat 80% relative humidity (RH) and stored in sealed glass vials.Pollen tubes elongated autotropically with atmospheric humidityapparently being a controlling factor in the process. Pollendehydrated at 50% RH and 25°C for 15-30 min neither germinatedin vitro, reduced tetrazolium chloride, nor set seed on malesterile plants. Rehydrating the pollen did not restore the capacityfor germination. SEM micrographs demonstrated that elongatingpollen tubes encircled the pollen grain and were contiguousto the surface. A fibrillar-like material existed on the exineof separated pollen grains at the point where the grains hadbeen previously attached.Copyright 1994, 1999 Academic Press Sorghum pollen, germination, seed-set, viability, scanning electron microscopy, Sorghum bicolor (L.) Moench 相似文献
14.
Guixiang Tang Wenjian Song Ling Xu Zonglai Jin Kasirajan Subrahmaniyan Weijun Zhou 《Acta Physiologiae Plantarum》2006,28(3):273-280
Experiments were conducted to study the influence of sowing seasons and drying methods on the seed vigour of two spring soybean
(Glycine max (L.) Merr.) cultivars. Two cultivars, ‘Huachun18’ and ‘Huachun 14’, were sown in three seasons viz., spring, summer and autumn and the harvested seeds were dried using three different methods. The results showed that soybean
sown in spring had a higher number of branches per plant, pods per branch and seed weight, and consequently resulted in higher
seed yields than that of soybean sown in autumn or summer seasons. Seeds sown in the autumn season had the lowest values of
electrical conductivity during seed imbibitions, higher peroxidase (POD) activity in germinated seedlings and lower contamination
by the seed-borne fungi on the MS medium, which indirectly improved the seed vigour, which was followed by summer sown seeds.
Seeds sown during the spring season resulted in poor seed vigour. In addition, the effect of drying methods on the seed vigour
was also clarified. Seeds that hung for four days before threshing and then air-dried had the poorest seed vigour which was
determined by germination, electrical conductivity, POD activity and seed borne fungal growth. There was no difference in
seed vigour between other methods, i.e. seeds threshed directly at harvest and then air-dried on a bamboo sifter or concrete floor. These results indicated that
autumn sowing soybean and the drying method in which seeds were threshed directly at harvest and then air-dried on a bamboo
sifter resulted in higher seed vigour. 相似文献
15.
16.
Consequences of Wheat Breeding on Nitrogen and Phosphorus Yield, Grain Nitrogen and Phosphorus Concentration and Associated Traits 总被引:12,自引:0,他引:12
Several studies have analysed the effects of wheat breedingon dry matter accumulation and partitioning, but little hasbeen done to understand the effects on nutrient economies. Theobjective of this study was to identify the changes producedby wheat breeding in the economy of nitrogen and phosphorusunder field conditions. Two experiments were carried out withseven genotypes (including a commercial hybrid) representingdifferent eras of plant breeding. Wheat breeding has increased grain nitrogen and phosphorus yieldbut total absorbed nutrients have not shown any trend duringthis century. The main attribute closely related to the increasein grain nitrogen and phosphorus yields was their harvest indices.The higher nutrient partitioning in the newer cultivars wasassociated with lower grain nitrogen and phosphorus concentrationsin their grains. Therefore, there was a negative effect of geneticimprovement in grain nitrogen and phosphorus concentrations.The main cause for the decreased concentration of these nutrientsin the grains of the modern cultivars appeared to be a dilutionby an even more increased dry matter partitioning. It is suggestedthat future breeding should be aimed to select for higher nitrogenuptake as a way to increase the level of this nutrient in grain.Copyright1995, 1999 Academic Press Triticum aestivum L., wheat breeding, genetic improvement, nitrogen, phosphorus, wheat, grain nitrogen concentration, grain phosphorus concentration 相似文献
17.
Sanhewe A. J.; Ellis R. H.; Hong T. D.; Wheeler T. R.; Batts G. R.; Hadley P.; Morison J. I. L. 《Journal of experimental botany》1996,47(5):631-637
Winter wheat (Triticum aestivum L.) cv. Hereward was grown inthe field in two double-walled polyethylene-covered tunnelswithin each of which a temperature gradient was superimposedon diurnal and seasonal fluctuations in temperature. The meantemperature between anthesis and harvest maturity varied from14.3 to 18.4C among plots within these tunnels. The CO2 concentrationwas controlled at different values in each tunnel; seasonalmean concentrations were 380 and 684 µmol CO2 mol1air. Crops were also grown outside the tunnels at ambient temperaturesand CO2. Samples of seeds were harvested sequentially from eachplot between anthesis and harvest maturity. Seed germinationand seed survival during subsequent air-dry storage were determinedfor each sample. The onset of both ability to germinate anddesiccation tolerance (ability to germinate after rapid desiccationto 1015% moisture content and subsequent rehydration)coincided in all environments. Full germination capacity (>97%, determined at 10C) was reached 418 d before theend of the seed-filling phase (mass maturity) in most cases.There was little or no decline in germination capacity duringsubsequent seed development and maturation. Differences in seedquality were evident, however, throughout seed development andmaturation when seed survival curves during subsequent storagewere compared. Potential longevity in air-dry storage (assessedby the value K1 of the seed viability equation) improved consistentlyboth before and after mass maturity. There was a significantpositive relation between the rate of increase in potentiallongevity (dK1Idt) and temperature (the minimum temperaturefor seed quality development was 4.8 C), but neither CO2 concentrationnor production within the polyethylene tunnels affected thisrelation. Key words: Wheat, Triticum aestivum L., seed development, seed longevity, carbon dioxide, temperature 相似文献
18.
The effects of storage conditions on the germination of developingmuskmelon (Cucumis melo L.) seeds were tested to determine whetherafter-ripening is required to obtain maximum seed vigour. Seedswere harvested at 5 d intervals from 35 (immature) to 60 (fullymature) days after anthesis (DAA), washed, dried, and storedat water contents of 3·3 to 19% (dry weight basis) at6, 20, or 30°C for up to one year. Germination was testedin water and in polyethylene glycol 8000 solutions ( 0·2to 1·2 MPa osmotic potential) at 15, 20, 25 or30°C. Germination percentages and rates (inverse of meantimes to radicle emergence) were compared to those of newlyharvested, washed and dried seeds. For 40 and 60 DAA seeds,one year of storage at 20°C and water contents <6·5%significantly increased germination percentages and rates at20°C, but had little effect on germination at 25 and 30°C.Storage reduced the estimated base temperature (Tb) and meanbase water potential (b) for germination of both 40 and 60 DAAseeds by approximately 5°C and 0·3 MPa, respectively.Immature 35 DAA seeds showed the greatest benefit from storageat 3 to 5% water content and 30°C, as germination percentagesand rates increased at all water potentials (). Storage underthese same conditions had little effect on the germination ofmature seeds in water, but increased germination percentagesand rates at reduced 's. Accelerated ageing for one month at30°C and water contents from 15 to 19° increased germinationrates and percentages of mature seeds at reduced 's, but longerdurations resulted in sharp declines in both parameters. Immatureseeds lost viability within one month under accelerated ageingconditions. An after-ripening period is required at all stagesof muskmelon seed development to expand the temperature andwater potential ranges allowing germination and to achieve maximumgerminability and vigour. Post-harvest dormancy is deepest atthe point of maximum seed dry weight accumulation and declinesthereafter, both in situ within the ripening fruit and duringdry storage. Key words: Muskmelon, Cucumis melo L., seed, development, dormancy, germination, vigour, after-ripening 相似文献
19.
In a field experiment, comprising four spring wheat cultivars,the frequency and final weight of the grains developing fromeach individual floret were determined in intact spikes andin spikes of which up to nine spikelets had been removed. Theextent of damage caused by the cutting procedure was estimated. Characteristic distributions of the frequencies and weightsof the individual grains were found for each cultivar. Removalof spikelets resulted, in most cases, in a small increase inthe number of grains and in a considerable increase in the weightof the grains of the remaining spikelets. These increases compensatedonly partially, and differently in the different cultivars,for the loss of the removed spikelets. Defoliation at the timeof earing caused a subsequent reduction in grain yield of intactspikes but no reduction in the yield of spikes from which ninespikelets had been removed. The removal of the upper floretsin each spikelet resulted in a certain increase in the weightof the two basal grains. It is concluded that an increase in the number of spikeletsper spike may reduce grain weight but will nevertheless contributeto yield. The number of grains per spikelet is cultivar dependentbut not causally associated with grain weight. Grain set indistal florets is expected to add rather small grains to thespike's yield. Under conditions of limited supplies it may causea reduction in the weight of the basal grains. Any increasein grain weight is anticipated to contribute to grain yieldand is not liable to affect spikelets per spike or grains perspikelet. Wheat cultivars, Triticum aestivum, growth of inflorescence, grain yield, spikelet number 相似文献
20.
Three primitive varieties of six-row barley from Nepal withdifferent degrees of awn development were compared with a bredEuropean cultivar, Senta, under glasshouse conditions, fromanthesis onwards. Apart from the flag leaf the last three mainshoot leaves of the Nepalese varieties were smaller than thoseof Senta, and the lower leaves did not become senescent duringthe first 3 weeks of grain filling. In all varieties the penultimateleaf was the major assimilatory organ but in Senta the relativecontribution of the awns increased with time as the leaves senescedand fixed almost half of the total 14C assimilated by the shoot.Whereas the supply of assimilate to the ear increased with timein Senta, the reverse was found for the Nepalese varieties andoverall a smaller proportion of assimilate was supplied to theear in these varieties. The grain yield of the main shoot earof the two Nepalese varieties studied in detail was one-thirdof that of Senta; there were only approximately half the numberof grains in each median and lateral row of the ear and thegrains were smaller. There was no correlation between the degreeof awn development or the pattern of awn arrangement withinthe ear and the size of individual grains. The total biomassof the Nepalese varieties was much lower than that of Sentabut there were no differences in the harvest index or in tillerproduction. However, the Nepalese varieties, in contrast toSenta, had a mean grain yield per tiller comparable to thatof their main shoot and thus it appears that selection by plantbreeding may have increased the dominance of the main shoot. Hordeum vulgare L., barley, primitive barley varieties, carbon dioxide assimilation, assimilate translocation, grain filling 相似文献