Scandinavian limnochronology of sediments and heavy metals

Sediment and peat accumulation rates as well as heavy metal fluxes are examined in Scandinavian lakes, marine coasts and peatlands. Modern accumulation rates are determined using Pb-210 and other chronological tools. Mean deposition rates in the past 150 ± 20 yrs are 3–95, 40–190 and 1–5 mg·cm^–2·yr^–1 for lakes, marine coasts and peatlands respectively. The Pb-210 fluxes at the investigated sites are quite variable. The lowest values are found for peatlands 0.04–0.20 pCi·cm^–2·yr^–1 while coastal marine environments showed 0.35–0.70 pCi·cm^–2·yr^–1. A wide range of variation of Pb-210 fluxes is obtained for the studied lake sediments 0.01–0.51 pCi·cm^–2·yr^–1.The chronological records of Pb, Zn, Cu, Cd and Hg show that heavy metal fluxes are much higher in coastal marine areas. Inland lakes and ombrotrophic peat-bogs indicate that Pb is introduced exclusively through the atmosphere and decrease exponentially from South to North Scandinavia. However, the other heavy metals are transported to freshwater systems by different hydrologic and atmospheric processes. Zn and Fe exhibit some correlation with lake-drainage area.     

Respiration from coarse wood litter in central Amazon forests

Respiration from coarse litter (trunks and large branches >10 cm diameter) was studied in central Amazon forests. Respiration ratesvaried over almost two orders of magnitude (1.003–0.014 µg Cg^–1 C min^–1, n = 61), and weresignificantly correlated with wood density (r² _adj= 0.42), and moisture content (r² _adj= 0.39). Additional samples taken from a nearby pasture indicatedthat wood moisture content was the most important factor controllingrespiration rates across sites (r² _adj =0.65). Based on average coarse litter wood density and moisture content,the mean long-term carbon loss rate due to respiration was estimated tobe 0.13 yr^–1 (range of 95% prediction interval(PI) = 0.11–0.15 yr^–1). Comparing meanrespiration rate with mean mass loss (decomposition) rate from aprevious study, respiratory emissions to the atmosphere from coarselitter were predicted to be 76% (95% PI =65–88%) of total carbon loss, or about 1.9 (95% PI= 1.6–2.2) Mg C ha^–1yr^–1. Optimum respiration activity corresponded toabout 2.5 g H₂O g^–1 dry wood, and severelyrestricted respiration to < 0.5 g H₂O g^–1dry wood. Respiration from coarse litter in central Amazon forests iscomparable in magnitude to decomposing fine surface litter (e.g. leaves,twigs) and is an important carbon cycling component when characterizingheterotrophic respiration budgets and net ecosystem exchange(NEE).     

Caesium-137 and lead-210 dating of recent sediments from Mondsee (Austria)

Mean annual sedimentation rates over the last 20–30 years were determined in the pre-alpine Mondsee (Upper Austria) using Cs-137 and Pb(Po)-210 profiles for sediment core dating and two natural sediment markers. Lower sedimentation rates of about 2–3 mm yr^–1 were observed in the central part of the lake near the shore at 18–20 m and in the southern part at 30 m depth. Higher sedimentation rates of 4–7 mm yr^–1 were found in the central part of the lake at 47 and 65 m and in the northern bay at 18 and 41 m depth. At both these sites the Pb-210 profiles were strongly disturbed in the upper zone of the sediment cores, whereas the Cs-137 pattern remained intact. The higher annual sediment accumulation rates can be explained only partly by deposition of allochthonous material discharged by the streams, enhanced eutrophication in these parts of the lake, erosion and sediment focusing by turbidity currents being also probable.     

Cycling of nitrogen in modern agricultural systems

Summary Agro-ecosystems have developed from mixed- and multiple-cropping systems with relatively closed N cycles to intensively managed monocultures with large N inputs in the form of commercial fertilizers. Cultivation of increasingly larger areas of land has resulted in substantial losses of soil organic matter and N. Also, the move from slash and burn agriculture to intensively ploughed systems has resulted in losses through increased erosion.The use of N fertilizers has increased rapidly toca. 60 Tg N yr^–1 (1980/81), which is equivalent to at least 40% of the N fixed biologically in all terrestrial systems and 36% more than is fixed in all croplands. On a global scale, the major losses of N from agro-ecosystems are estimated to be: harvest, 30 Tg; leaching, 2 Tg; erosion, 2–20 Tg; denitrification 1–44 Tg; and ammonia volatilization, 13–23 Tg. However, the data base is very crude and several estimates may be wrong by as much as one order of magnitude.Additions of N fertilizers have both direct and indirect effects on soil microorganisms. The possible importance of such effects is briefly discussed and a specific example is given on long-term effects on soil microbial biomass and nitrification rates in 27-year-old cropping systems with different N additions: (i) 0 kg N ha^–1 yr^–1, (ii) 80 kg N ha^–1 yr^–1, (iii) farmyard manureca. 80 kg N ha^–1 yr^–1.Few detailed N budgets exist for agro-ecosystems, despite its major importance as a limiting plant nutrient and the large losses of N from such systems. In conclusion, preliminary nitrogen budgets for four cropping systems (barley receiving 0 or 120 kg N ha^–1 yr^–1; meadow fescue ley with 200 kg N ha^–1 yr^–1 and a lucerne ley) are presented, with special attention to N flow through the soil organisms.Keynote address     

Experimental studies of rapid bioerosion of coral reefs in the Galápagos Islands

Experimental carbonate blocks of coral skeleton,Porites lobata (PL), and cathedral limestone (LS) were deployed for 14.8 months at shallow (5–6 m) and deep (11–13m) depths on a severely bioeroded coral reef, Champion Island, Galápagos Islands, Ecuador. Sea urchins (Eucidaris thouarsii) were significantly more abundant at shallow versus deep sites.Porites lobata blocks lost an average of 25.4 kg m^–2yr^–1 (23.71 m^–2yr^–1 or 60.5% decrease yr^–1). Losses did not vary significantly at depths tested. Internal bioeroders excavated an average of 2.6 kg m^–2 yr^–1 (2.41 m^–2 yr^–1 or 0.6% decrease yr^–1), while external bioeroders removed an average of 22.8 kg m^–2 yr^–1). (21.31 m^–2 yr^–1). or 59.9% decrease yr^–1). few encrusting organisms were observed on the PL blocks. Cathedral limestone blocks lost an average of 4.1 kg m^–2 yr^–1). (1.81 m^–2 yr^–1). or 4.6% decrease yr-'), also with no relation to depth. Internal bioeroders excavated an average of 0.6 kg m^–2 yr^–1). (0.31 m^–2 yr^–1). or 0.7% decrease yr^–1). and external bioeroders removed an average of 3.5 kg m^–2 yr^–1). (1.51 m^–2 yr^–1). or 3.9% decrease yr^–1). from the LS blocks. Most (57.6%) encrustation occurred on the bottom of LS blocks, and there was more accretion on block bottoms in deep (61.4 mg cm^–2 yr^–1). versus shallow (35.0 mg cm^–2 yr^–1) sites. External bioerosion reduced the average height of the reef framework by 0.2 cm yr^–1). for hard substrata (represented by LS) and 2.3 cm yr^–1). for soft substrata (represented by PL). The results of this study suggest that coral reef frameworks in the Galápagos Islands are in serious jeopardy. If rates of coral recruitment do not increase, and if rates of bioerosion do not decline, coral reefs in the Galápagos Islands could be eliminated entirely.     

The soil fauna of a beech forest on limestone: trophic structure and energy budget

Summary The soil fauna of a mull beech forest on lime-stone in southern Lower Saxony (West Germany) was sampled quantitatively. Biomass estimates, trophic characteristics, and measurement and calculation of the energetic parameters of the constituent animal populations were used to construct an energy budget of the total heterotrophic subsystem of the forest. Mean annual zoomass amounted to about 15 g d wt m^–2; earthworms (about 10 g d wt m^–2) and other groups of the macrofauna were dominant. Protozoa constituted about 1.5 g d wt m^–2. Relative distribution of zoomass among the trophic categories was 50% macrosaprophages, 30% microsaprophages, 12% microphytophages, and 4% zoophages. Total annual consumption rate of the saprophagous and microphytophagous soil fauna (6328 and 4096 kJ m^–2 yr^–1, respectively) was of the same order of magnitude as annual litter fall (canopy leaves 6124 kJ m^–2 yr^–1, flowers and fruits 944 kJ m^–2 yr^–1, herbs 1839 kJ m^–2 yr^–1, fine woody material 870 kJ m^–2 yr^–1, tree roots 3404 kJ m^–2 yr^–1, without coarse woody litter). Primary decomposers (macrosaprophages) were the key group for litter comminution and translocation onto and into the soil, thus contributing to the high decomposition rate (k=0.8) for leaf litter. Consumption rates of the other trophic groups were (values as kJ m^–2 yr^–1): bacteriophages 2954, micromycophages 416, zoophages 153. Grazing pressure of macrophytophages (including rhizophages) was low. Faeces input from the canopy layer was not significant. Grazing pressure on soil microflora almost equalled microbial biomass; hence, a large fraction of microbial production is channelled into the animal component. Predator pressure on soil animals is high, as a comparison between consumption rates by zoophages and production by potential prey — mainly microsaprophages, microphytophages and zoophages — demonstrated. Soil animals contributed only about 11% to heterotrophic respiration. However, there is evidence that animals are important driving variables for matter and energy transfer: key processes are the transformation of dead organic material and grazing on the microflora. It is hypothesized that the soil macrosaprophages are donor-limited.     

Watershed Sediment Losses to Lakes Accelerating Despite Agricultural Soil Conservation Efforts

Agricultural soil loss and deposition in aquatic ecosystems is a problem that impairs water quality worldwide and is costly to agriculture and food supplies. In the US, for example, billions of dollars have subsidized soil and water conservation practices in agricultural landscapes over the past decades. We used paleolimnological methods to reconstruct trends in sedimentation related to human-induced landscape change in 32 lakes in the intensively agricultural region of the Midwestern United States. Despite erosion control efforts, we found accelerating increases in sediment deposition from erosion; median erosion loss since 1800 has been 15.4 tons ha⁻¹. Sediment deposition from erosion increased >6-fold, from 149 g m⁻² yr⁻¹ in 1850 to 986 g m⁻² yr⁻¹ by 2010. Average time to accumulate one mm of sediment decreased from 631 days before European settlement (ca. 1850) to 59 days mm⁻¹ at present. Most of this sediment was deposited in the last 50 years and is related to agricultural intensification rather than land clearance or predominance of agricultural lands. In the face of these intensive agricultural practices, traditional soil conservation programs have not decelerated downstream losses. Despite large erosion control subsidies, erosion and declining water quality continue, thus new approaches are needed to mitigate erosion and water degradation.     

Residual phosphorus in runoff from successional forest on abandoned agricultural land: 2. Hydrological and soluble reactive P Budgets

Residual P from historical farm practices hasbeen linked to elevated soluble reactivephosphorus (SRP) transport in runoff from afield study site in the Catskills Mountains,New York, U.S.A., with a P source assay indicatingthat successional forest floor biomass was themajor contributor to runoff SRP. In thispaper, we assemble hydrological and SRP budgetsthat indicate net SRP loss of 0.123 kgha^–1 yr^–1 occurs from the site(composed of 0.044 kg ha^–1 yr^–1precipitation input, with 0.143 kg ha^–1yr^–1 and 0.024 kg ha^–1 yr^–1losses in runoff and groundwater,respectively). These findings contrast withconservative P cycling reported for undisturbedforests. Coupled hydrological and SRP data areanalyzed suggesting that catchment ambient andequilibrium SRP concentrations corresponding togroundwater and long-term average runoffconcentrations are in the range capable ofcontributing to eutrophication of receivingwaters. A physically based variable sourcearea hydrological model is tested to simulateSRP export using deterministic concentrations. The three-layer model (surface runoff, shallowlateral flow, and groundwater) is parameterizedusing spatially distributed data fromadditional P source assays and fieldhydrological monitoring for the site. Differences in simulated and observed outflowand SRP export are partially explained byforest evapotranspiration and frozen soilprocesses. The field data, SRP budgets andsimulations show that sufficient residual Ppools exist to prolong net SRP loss rates untilecosystem processes re-establish moreconservative P cycling.     

Nitrogen fixation and nitrogen balance studies in Rhizobium-VA mycorrhiza inoculated legume-grass association by15N isotope dilution technique under a field condition

A mixed pasture comprising of buffel grass and a legume siratro was studied under field condition for a two-year period to know the fodder yield increase, nitrogen fixation and nitrogen balance with and without the inoculation of VA mycorrhiza to grass and Rhizobium to legume component.¹⁵N dilution technique was followed using labelled ammonium sulphate. The data showed that during the first year of the above study combined inoculation of VA mycorrhiza and Rhizobium to grass and legume respectively significantly increased the total dry matter (DM) (23,900 kg ha^–1 yr^–1) and total N content (308 kg ha^–1 yr^–1) of the mixed pasture over the uninoculated mixture. However, the above increase due to combined inoculation was maximum during second year with respect to DM yield (28,200 kg ha^–1 yr^–1), but the total N harvested through grass-legume mixture was comparatively lower than the first year (297 kg ha^–1 yr^–1). The amount of biologically fixed N was highest in the first year (79 kg ha^–1 yr^–1) and showed a very drastic reduction at the end of second year (39 kg ha^–1 yr^–1). A positive nitrogen balance was observed in the grass-legume mixture irrespective of inoculation of VA mycorrhiza and/or Rhizobium.     

Nitrogen fixation by white clover in pastures grazed by dairy cows: Temporal variation and effects of nitrogen fertilization

Effects of rate of nitrogen (N) fertilizer and stocking rate on production and N₂ fixation by white clover (Trifolium repens L.) grown with perennial ryegrass (Lolium perenne L.) were determined over 5 years in farmlets near Hamilton, New Zealand. Three farmlets carried 3.3 dairy cows ha^–1 and received urea at 0, 200 or 400 kg N ha^–1 yr^–1 in 8–10 split applications. A fourth farmlet received 400 kg N ha^–1 yr^–1 and had 4.4 cows ha^–1.There was large variation in annual clover production and total N₂ fixation, which in the 0 N treatment ranged from 9 to 20% clover content in pasture and from 79 to 212 kg N fixed ha^–1 yr^–1. Despite this variation, total pasture production in the 0 N treatment remained at 75–85% of that in the 400 N treatments in all years, due in part to the moderating effect of carry-over of fixed N between years.Fertilizer N application decreased the average proportion of clover N derived from N₂ fixation (P_N; estimated by ¹⁵N dilution) from 77% in the 0 N treatment to 43–48% in the 400 N treatments. The corresponding average total N₂ fixation decreased from 154 kg N ha^–1 yr^–1 to 39–53 kg N ha^–1 yr^–1. This includes N₂ fixation in clover tissue below grazing height estimated at 70% of N₂ fixation in above grazing height tissue, based on associated measurements, and confirmed by field N balance calculations. Effects of N fertilizer on clover growth and N₂ fixation were greatest in spring and summer. In autumn, the 200 N treatment grew more clover than the 0 N treatment and N₂ fixation was the same. This was attributed to more severe grazing during summer in the 0 N treatment, resulting in higher surface soil temperatures and a deleterious effect on clover stolons.In the 400 N treatments, a 33% increase in cow stocking rate tended to decrease P_N from 48 to 43% due to more N cycling in excreta, but resulted in up to 2-fold more clover dry matter and N₂ fixation because lower pasture mass reduced grass competition, particularly during spring.     

Retention of nutrients in river basins

In Denmark, as in many other European countries, the diffuse losses of nitrogen (N) and phosphorus (P) from the rural landscape are the major causes of surface water eutrophication and groundwater pollution. The export of total N and total P from the Gjern river basin amounted to 18.2 kg ha^–1 and 0.63 kg P ha^–1 during June 1994 to May 1995. Diffuse losses of N and P from agricultural areas were the main nutrient source in the river basin contributing 76% and 51%, respectively, of the total export.Investigations of nutrient cycling in the Gjern river basin have revealed the importance of permanent nutrient sinks (denitrification and overbank sedimentation) and temporary nutrient storage in watercourses. Temporary retention of N and P in the watercourses thus amounted to 7.2–16.1 g N m^–2 yr^–1 and 3.7–8.3 g P m^–2 yr–1 during low-flow periods. Deposition of P on temporarily flooded riparian areas amounted from 0.16 to 6.50 g P m^–2 during single irrigation and overbank flood events, whereas denitrification of nitrate amounted on average to 7.96 kg N yr^–1 per running metre watercourse in a minerotrophic fen and 1.53 kg N yr^–1 per linear metre watercourse in a wet meadow. On average, annual retention of N and P in 18 Danish shallow lakes amounted to 32.5 g N m^–2 yr^–1 and 0.30 g P m^–2 yr^–1, respectively, during the period 1989–1995.The results indicate that permanent nutrient sinks and temporary nutrient storage in river systems represent an important component of river basin nutrient budgets. Model estimates of the natural retention potential of the Gjern river basin revealed an increase from 38.8 to 81.4 tonnes yr^–1 and that P-retention increased from –0.80 to 0.90 tonnes yr^–1 following restoration of the water courses, riparian areas and a shallow lake. Catchment management measures such as nature restoration at the river basin scale can thus help to combat diffuse nutrient pollution.     

Exchange of N-gases at the Höglwald Forest – A summary

During 4 years continuous measurements of N-trace gas exchange were carried out at the forest floor-atmosphere interface at the Höglwald Forest that is highly affected by atmospheric N-deposition. The measurements included spruce control, spruce limed and beech sites. Based on these field measurements and on intensive laboratory measurements of N₂-emissions from the soils of the beech and spruce control sites, a total balance of N-gas emissions was calculated. NO₂-deposition was in a range of –1.6 –2.9 kg N ha^–1 yr^–1 and no huge differences between the different sites could be demonstrated. In contrast to NO₂-deposition, NO- and N₂O-emissions showed a huge variability among the different sites. NO emissions were highest at the spruce control site (6.4–9.1 kg N ha^–1 yr^–1), lowest at the beech site (2.3–3.5 kg N ha^–1 yr^–1) and intermediate at the limed spruce site (3.4–5.4 kg N ha^–1 yr^–1). With regard to N₂O-emissions, the following ranking between the sites was found: beech (1.6–6.6 kg N ha^–1 yr^–1) >> spruce limed (0.7–4.0 kg N ha^–1 yr^–1) > spruce control (0.4–3.1 kg N ha^–1 yr^–1). Average N-trace gas emissions (NO, NO₂, N₂O) for the years 1994–1997 were 6.8 kg N ha^–1 yr^–1 at the spruce control site, 3.6 kg N ha^–1 yr^–1 at the limed spruce site and 4.5 kg N ha^–1 yr^–1 at the beech site. Considering N₂-losses, which were significantly higher at the beech (12.4 kg N ha^–1 yr^–1) than at the spruce control site (7.2 kg N ha^–1 yr^–1), the magnitude of total gaseous N losses, i.e. N₂-N + NO-N + NO₂-N + N₂O-N, could be calculated for the first time for a forest ecosystem. Total gaseous N-losses were 14.0 kg N ha^–1 yr^–1 at the spruce control site and 15.5 kg N ha^–1 yr^–1 at the beech site, respectively. In view of the huge interannual variability of N-trace gas fluxes and the pronounced site differences in N-gas emissions it is concluded that more research is needed in order to fully understand patterns of microbial N-cycling and N-gas production/emission in forest ecosystems and mechanisms of reactions of forest ecosystems to the ecological stress factor of atmospheric N-input.     

Rapid Losses of Surface Elevation following Tree Girdling and Cutting in Tropical Mangroves

The importance of mangrove forests in carbon sequestration and coastal protection has been widely acknowledged. Large-scale damage of these forests, caused by hurricanes or clear felling, can enhance vulnerability to erosion, subsidence and rapid carbon losses. However, it is unclear how small-scale logging might impact on mangrove functions and services. We experimentally investigated the impact of small-scale tree removal on surface elevation and carbon dynamics in a mangrove forest at Gazi bay, Kenya. The trees in five plots of a Rhizophora mucronata (Lam.) forest were first girdled and then cut. Another set of five plots at the same site served as controls. Treatment induced significant, rapid subsidence (−32.1±8.4 mm yr⁻¹ compared with surface elevation changes of +4.2±1.4 mm yr⁻¹ in controls). Subsidence in treated plots was likely due to collapse and decomposition of dying roots and sediment compaction as evidenced from increased sediment bulk density. Sediment effluxes of CO₂ and CH₄ increased significantly, especially their heterotrophic component, suggesting enhanced organic matter decomposition. Estimates of total excess fluxes from treated compared with control plots were 25.3±7.4 tCO₂ ha⁻¹ yr⁻¹ (using surface carbon efflux) and 35.6±76.9 tCO₂ ha⁻¹ yr⁻¹ (using surface elevation losses and sediment properties). Whilst such losses might not be permanent (provided cut areas recover), observed rapid subsidence and enhanced decomposition of soil sediment organic matter caused by small-scale harvesting offers important lessons for mangrove management. In particular mangrove managers need to carefully consider the trade-offs between extracting mangrove wood and losing other mangrove services, particularly shoreline stabilization, coastal protection and carbon storage.     

Ecosystem respiration and organic carbon processing in a large,tidally influenced river: the Hudson River

We estimated whole-ecosystem rates of respiration over a 40-km stretch of the tidally influenced freshwater Hudson River every 2 to 3 weeks from May through November. We measured in situ concentrations of oxygen over depth at dusk and dawn at 10 stations spaced over this interval. The use of multiple stations allowed for the consideration of the influence of tidal advection of water masses. Respiration was estimated from the decrease in oxygen overnight with a correction for diffusive exchange of oxygen with the atmosphere. We estimated this flux of oxygen to or from the atmosphere using the measured oxygen gradient and a transfer velocity model which is a function of wind velocity.Integration of the data for the period of May through November yields an estimate of whole-ecosystem respiration of 591 g C m^–2 (S.E. = 66). That the standard error of this estimate is relatively low (11% of the estimate) indicates that the use of multiple stations adequately deals with error introduced through the advection of water between stations. The logarithm of average daily respiration rate was correlated with average daily temperature (p = 0.007;r ² = 0.62). We used this temperature-respiration relationship to derive an estimate of the annual respiration rate of 755 g C m^–2 yr^–1 (S.E. = 72). This estimate is moderately sensitive to the estimated flux of oxygen between the atmosphere and water; using the lower and upper 95% confidence limits of our model relating the transfer velocity of oxygen to wind speed gives a range of annual respiration estimates from 665 g C m^–2 yr^–1 to 984 g C m^–2 yr^–1.The river is strongly heterotrophic, with most respiration driven by allochthonous inputs of organic matter from terrestrial ecosystems. The majority of the allochthonous inputs to the river (over 60%) are apparently metabolized within the river. Any change in allochthonous inputs due to changes in land use or climate patterns would be expected to alter the oxygen dynamics and energy flow within this tidally influenced river.     

Hydrological and nutrient budgets of freshwater and estuarine wetlands of Taylor Slough in Southern Everglades, Florida (U.S.A.)

Hydrological restoration of the Southern Everglades will result in increased freshwater flow to the freshwater and estuarine wetlands bordering Florida Bay. We evaluated the contribution of surface freshwater runoff versus atmospheric deposition and ground water on the water and nutrient budgets of these wetlands. These estimates were used to assess the importance of hydrologic inputs and losses relative to sediment burial, denitrification, and nitrogen fixation. We calculated seasonal inputs and outputs of water, total phosphorus (TP) and total nitrogen (TN) from surface water, precipitation, and evapotranspiration in the Taylor Slough/C-111 basin wetlands for 1.5 years. Atmospheric deposition was the dominant source of water and TP for these oligotrophic, phosphorus-limited wetlands. Surface water was the major TN source of during the wet season, but on an annual basis was equal to the atmospheric TN deposition. We calculated a net annual import of 31.4 mg m^–2 yr^–1 P and 694 mg m^–2 yr^–1N into the wetland from hydrologic sources. Hydrologic import of P was within range of estimates of sediment P burial (33–70 mg m^–2 yr^–1 P), while sediment burial of N (1890–4027 mg m^–2 yr^–1 N) greatly exceeded estimated hydrologic N import. High nitrogen fixation rates or an underestimation of groundwater N flux may explain the discrepancy between estimates of hydrologic N import and sediment N burial rates.     

Clipperton Atoll (eastern Pacific): oceanography,geomorphology, reef-building coral ecology and biogeography

Coral reef geomorphology and community composition were investigated in the tropical northeastern Pacific during April 1994. Three areas were surveyed in the Revillagigedo Islands (Mexico), and an intensive study was conducted on Clipperton Atoll (1,300 km SW of Acapulco), including macro-scale surface circulation, sea surface temperature (SST) climatology, geomorphology, coral community structure, zonation, and biogeography. Satellite-tracked drifter buoys from 1979–1993 demonstrated complex patterns of surface circulation with dominantly easterly flow (North Equatorial Counter Current, NECC), but also westerly currents (South Equatorial Current, SEC) that could transport propagules to Clipperton from both central and eastern Pacific regions. The northernmost latitude reached by the NECC is not influenced by El Niño-Southern Oscillation (ENSO) events, but easterly flow velocity evidently is accelerated at such times. Maximum NECC flow rates indicate that the eastern Pacific barrier can be bridged in 60 to 120 days. SST anomalies at Clipperton occur during ENSO events and were greater at Clipperton in 1987 than during 1982–1983. Shallow (15–18 m)and deep (50–58 m) terraces are present around most of Clipperton, probably representing Modern and late Pleistocene sea level stands. Although Clipperton is a well developed atoll with high coral cover, the reef-building fauna is depauperate, consisting of only 7 species of scleractinian corals belonging to the generaPocillopora, Porites, Pavona andLeptoseris, and 1 species of hydrocoral in the genusMillepora. The identities of the one Pocilpopora species and one of the twoPorites species are still unknown. Two of the remaining scleractinians (Pavona minuta, Leptoseris scabra) and the hydrocoral (Millepora exaesa), all formerly known from central and western Pacific localities, represent new eastern Pacific records. Scleractinian corals predominate (10–100% cover) over insular shelf depths of 8 to 60m, and crustose coralline algae are dominant (5–40% cover) from 0.5 to 7m. Spur and groove features, constructed of alternating frameworks ofPocillopora andPorites, and veneered with crustose coralline algae, are generally well developed around most atoll exposures. Although crustose coralline algae predominate in the breaker zone (with up to 100% cover), a prominent algal ridge is absent with only a slight buildup (ca. 10 cm) to seaward. Frequent grazing by the pufferfishArothron meleagris results in the removal of large amounts of live tissue and skeleton fromPorites lobata. Acanthaster planci is present, but rare. The grazing of large diadematid sea urchins, (2 species each ofDiadema andEchinothrix) on dead corals cause extensive erosion in some areas. Large numbers of corals on the 15–18 m terrace had recently suffered partial (P. lobata, 60–70% maximum of all colonies sampled) or total (Pocillopora sp., 80% maximum) mortality. The lengths of regenerating knobs and the rates of linear skeletal growth inP. lobata, determined by sclerochronologic analysis, indicated a period of stress during 1987. Massive skeletal growth is significantly higher at intermediate (16–17 m) than shallow (6–8 m) depths with mean extension rates of 1.5 mm yr^–1 inP. lobata and 1.4 mm yr^–1 inP. minuta at intermediate depths. Skeletal growth inP. lobata was depressed during the 1987 El Nifio event at Clipperton. The branching coralPocillopora sp. demonstrated high and similar skeletal growth rates at both shallow (25.4 mm yr^–1) and intermediate (26.5 mm yr^–1) depths. The presence of widely distributed Indo-Pacific zooxanthellate corals at Clipperton and the Revillagigedo Islands indicates that these NE Pacific Islands probably serve as a stepping stone for dispersal into the far eastern Pacific region.     

Nitrogen mineralization in heathland ecosystems dominated by different plant species

Net N mineralization rates were measured in heathlands still dominated by ericaceous dwarf shrubs (Calluna vulgaris or Erica tetralix) and in heathlands that have become dominated by grasses (Molinia caerulea or Deschampsia flexuosa). Net N mineralization was measuredin situ by sequential soil incubations during the year. In the wet area (gravimetric soil moisture content 74–130%), the net N mineralization rates were 4.4 g N m^–2 yr^–1 in the Erica soil and 7.8 g N m^–2 yr^–1 in the Molinia soil. The net nitrification rate was negligibly slow in either soil. In the dry area (gravimetric soil moisture content 7–38%), net N mineralization rates were 6.2 g N M-2 yr^–1 in the Calluna soil, 10.9 g N m^–2 yr^–1 in the Molinia soil and 12.6 g N m^–2 yr^–1 in the Deschampsia soil. The Calluna soil was consistently drier throughout the year, which may partly explain its slower mineralization rate. Net nitrification was 0.3 g N m^–2 yr^–1 in the Calluna soil, 3.6 g N m^–2 yr^–1 in the Molinia soil and 5.4 g N m^–2 yr^–1 in the Deschampsia soil. The net nitrification rate increased proportionally with the net N mineralization rate suggesting ammonium availability may control nitrification rates in these soils. In the dry area, the faster net N mineralization rates in sites dominated by grasses than in the site dominated by Calluna may be explained by the greater amounts of organic N in the soil of sites dominated by grasses. In both areas, however, the net amount of N mineralized per gram total soil N was greater in sites dominated by Molinia or Deschampsia than in sites dominated by Calluna or Erica. This suggests that in heathlands invaded by grasses the quality of the soil organic matter may be increased resulting in more rapid rates of soil N cycling.     

Diazotrophy in Alluvial Meadows of Subarctic River Systems

There is currently limited understanding of the contribution of biological N₂ fixation (diazotrophy) to the N budget of large river systems. This natural source of N in boreal river systems may partially explain the sustained productivity of river floodplains in Northern Europe where winter fodder was harvested for centuries without fertilizer amendments. In much of the world, anthropogenic pollution and river regulation have nearly eliminated opportunities to study natural processes that shaped early nutrient dynamics of large river systems; however, pristine conditions in northern Fennoscandia allow for the retrospective evaluation of key biochemical processes of historical significance. We investigated biological N₂ fixation (diazotrophy) as a potential source of nitrogen fertility at 71 independent floodplain sites along 10 rivers and conducted seasonal and intensive analyses at a subset of these sites. Biological N₂ fixation occurred in all floodplains, averaged 24.5 kg N ha⁻¹ yr⁻¹ and was down regulated from over 60 kg N ha⁻¹ yr⁻¹ to 0 kg N ha⁻¹ yr⁻¹ by river N pollution. A diversity of N₂-fixing cyanobacteria was found to colonize surface detritus in the floodplains. The data provide evidence for N₂ fixation to be a fundamental source of new N that may have sustained fertility at alluvial sites along subarctic rivers. Such data may have implications for the interpretation of ancient agricultural development and the design of contemporary low-input agroecosystems.     

Atmospheric deposition of nutrients to the North Atlantic Basin

Atmospheric chemical models are used to estimate the deposition rate of various inorganic oxides of nitrogen (NO_y), reduced nitrogen species (NH_x) and mineral dust to the North Atlantic Ocean (NAO). The estimated deposition of NO_y to the NAO (excluding the coastal ocean) and the Caribbean is 360 × 10⁹ Moles-N m^–2 yr^–1 (5.0 Tg N); this is equivalent to about 13% of the estimated global emission rate (natural and anthropogenic) and a quarter of the emission rate from sources in North America and Europe. In the case of NH_x, 258 Moles-N m^–2 yr^–1 (3.6 Tg N) are deposited to the NAO and the Caribbean; this is about 6% of the global continental emissions. There is relatively little data on the deposition rate of organic nitrogen species; nonetheless, this evidence suggests that concentrations and deposition rates are comparable to those for inorganic nitrogen.Because of anthropogenic emissions, the present-day deposition rate of NO_y to the NAO is about five times greater than pre-industrial times largely due to emissions from energy production and biomass burning. The present-day emissions of NH_x from continental anthropogenic sources are about four-to-five times greater than natural sources, mostly due to the impact of emissions from animal wastes associated with food production. Indeed, present-day emissions of NH_x from animal waste are estimated to be about 10 times greater than the pre-human era. The deposition rate of mineral dust to the NAO is about 170 Tg yr^–1; deposited with the dust (assuming average crustal abundances) is about 6 Tg yr^–1 of Fe and 0.2 Tg yr^–1 of P. Dust deposition in the NAO is almost completely attributable to transport from North African sources; a substantial fraction of the dust over the NAO is probably mobilized as a consequence of land use practices in arid regions and, consequently, it should be regarded as a pollutant.     

The effect of a single application of cow urine on annual N2 fixation under varying simulated grazing intensity, as measured by four 15N isotope techniques

The effects of dairy cow urine and defoliation severity on biological nitrogen fixation and pasture production of a mixed ryegrass-white clover sward were investigated over 12 months using mowing for defoliation. A single application of urine (equivalent to 746 kg N ha^–1), was applied in late spring to plots immediately after light and moderately-severe defoliation (35 mm and 85 mm cutting heights, respectively) treatments were imposed. Estimates of percentage clover N derived from N₂ fixation (%Ndfa) were compared by labelling the soil with ¹⁵N either by applying a low rate of ¹⁵N-labelled ammonium sulphate, immobilising ¹⁵N in soil organic matter, adding ¹⁵N to applied urine, or by utilising the small differences in natural abundance of ¹⁵N in soil. Urine application increased annual grass production by 85%, but had little effect on annual clover production. However, urine caused a marked decline in %Ndfa (using an average of all ¹⁵N methods) from 84% to a low of 22% by 108 days, with recovery to control levels taking almost a year. As a result, total N fixed (in above ground clover herbage) was reduced from 232 to 145 kg N ha^–1 yr^–1. Moderately–severe defoliation had no immediate effect on N₂ fixation, but after 108 days the %Ndfa was consistently higher than light defoliation during summer and autumn, and increased by up to 18%, coinciding with an increase in growth of weeds and summer-grass species. Annual N₂ fixation was 218 kg N ha^–1 yr^–1 under moderately-severe defoliation compared to 160 kg N ha^–1 yr^–1 under light defoliation. Estimates of %Ndfa were generally similar when ¹⁵N-labelled or immobilised ¹⁵N were used to label soil regardless of urine and defoliation severity. The natural abundance technique gave highly variable estimates of %Ndfa (–56 to 24%) during the first 23 days after urine application but, thereafter, estimates of %Ndfa were similar to those using ¹⁵N-labelling methods. In contrast, in urine treated plots the use of ¹⁵N-labelled urine gave estimates of %Ndfa that were 20–30% below values calculated using conventional ¹⁵N-labelling during the first 161 days. These differences were probably due to differences in the rooting depth between ryegrass and white clover in conjunction with treatment differences in ¹⁵N distribution with depth. This study shows that urine has a prolonged effect on reducing N₂ fixation in pasture. In addition, defoliation severity is a potential pasture management tool for strategically enhancing N₂ fixation.