Chris Somerville

Chris Somerville is Director of the Carnegie Institution Department of Plant Biology and a professor of Biological Sciences at Stanford University. He grew up in the Canadian north and was educated at the University of Alberta. He was one of the early Arabidopsis enthusiasts and has used the plant to study a variety of topics in cell and molecular biology. He is currently exploring the complexities of plant cell wall biogenesis.     

Nanosecond Electroporation: Another Look

As the medical field moves from treatment of diseases with drugs to treatment with genes, safe and efficient gene delivery systems are needed to make this transition. One such safe, non-viral, and efficient gene delivery system is electroporation (electrogenetherapy). Exciting discoveries using electroporation could make this technique applicable to drug and vaccine delivery in addition to gene delivery. Typically milli and microsecond pulses have been used for electroporation. Recently, the use of nanosecond electrical pulses (10-300 ns) at very high magnitudes (10-300 kV/cm) has been studied for direct DNA transfer to the nucleus in vitro. This article reviews the work done using high-intensity nanosecond pulses, termed as nanosecond electroporation (nsEP), in electroporation gene delivery systems.