Do Feeding Resources Induce the Adoption of Resource Defence Polygyny in a Lekking Butterfly?

Among polygynous species, males often compete for the possession of mating sites to increase their reproductive success. Weaker individuals frequently adopt alternative non‐territorial mate‐locating tactics, but the adoption of alternative territorial tactics may also occur. Although alternative tactics with territory defence are less common in arthropods, factors that drive its adoption may provide information to understand the organization of different territorial mating systems in the group. Here we investigate the adoption of resource‐based territoriality as an alternative to a non‐resource‐based one by males of the butterfly Paryphthimoides phronius. Male P. phronius commonly defend sunny clearings lacking feeding resources in the forest edge (non‐resource‐based territoriality). However, after experimentally offering fermenting fruit in previously undefended sites, we showed that males also adopt a resource defence tactic. Males in territories with fermenting fruits apparently feed on this resource when they are not defending the territory. However, males in sites without resources did not migrate to territories with resources when given the opportunity. To our knowledge, this is the first experimental investigation to show a non‐resource‐ and an alternative resource‐based mate‐locating tactic in a butterfly. We suggest that this behavioural flexibility may represent an important step to understand the ecological factors responsible for the organization and evolution of different territorial mating systems in insects.     

How Frequent Are Eating Disturbances in the Population? Norms of the Eating Disorder Examination-Questionnaire

Objective

The Eating Disorder Examination-Questionnaire (EDE-Q) is a self-report instrument assessing the specific psychopathology and key behaviors of eating disorders. This study sought to determine the prevalence of eating disturbances, and to provide psychometric properties and norms of the EDE-Q, in a representative German population sample.

Methods

A total of 2520 individuals (1166 men, 1354 women) were assessed with the EDE-Q.

Results

Eating disorder psychopathology was higher and most key behaviors were more prevalent in women than in men. Psychopathology declined with age ≥65 in both sexes, and showed a peak at age 55–64 in men. Overall, 5.9% of the women and 1.5% of the men revealed eating disturbances. The prevalence of eating disturbances decreased with age in women and was significantly higher in obese than in normal-weight individuals. Psychometric analyses showed favorable item characteristics. Internal consistencies of EDE-Q composite scores were ≥.80 for women and ≥.70 for men. The factor structure of the EDE-Q was partially reproduced. Sex- and age-specific population norms are reported.

Discussion

This study provides population norms of the EDE-Q for both sexes and across the age range, demonstrates demographic variations in symptomatology, and reveals satisfactory psychometric properties. Further research is warranted on eating disturbances in older adults.     

Are We Losing the Best Parts of Our Protected Areas in Tropical Mountains?

Protected areas (PAs) on tropical mountains undergo greater forest destruction in their lower altitudes. We compared the extent of forested, nonforested, and fragmented areas between lowland (<1000 m asl) and montane zones of the Blue Mountains inside the Blue and John Crow Mountains National Park established in Jamaica in 1993. We found that in 2008, inside the montane zone, only 4 percent of forest was cleared, and forest fragmentation was minimal. In the lowland zone, however, the percentage of forest cleared was seven times as high, and the density of fragments was 11-fold higher. We established twenty-five 0.04 ha lowland plots; ordination of tree species composition in these plots reflected a rainfall gradient, showing that plots on the wetter northern side of the Blue Mountains were floristically different from those on the drier southern side. The conservation value of the remaining lowland forest is high because of its high endemism (18% of species in our plots) and beta diversity. In addition, IUCN Red List data show that about 71 percent of threatened tree species in the Blue Mountains grow in the lowland region, 92 percent of which are endemic. From these findings, we identify a ‘protected area hotspot zone’, which lies between the PA boundary and the core high-altitude zone, and which should be instituted in IUCN categories I and II PAs.     

On the Occlusal Fit of Tribosphenic Molars: Are We Underestimating Species Diversity in the Mesozoic?

The complex occlusal fits between tribosphenic teeth are a rich source of information for taxonomic, phylogenetic, and evolutionary analysis. The degree of fit between upper and lower cheek teeth has been used to refer specimens to species-level taxa, but statistical data on occlusal fit in relation to taxonomic identity have been lacking. We used landmarks on upper and lower first molars of 20 bat populations representing 16 species to assess the degree of occlusal fit (1) between teeth from the same individual; (2) between teeth from different individuals belonging to the same populations; and (3) between teeth belonging to different populations. We found that the fit of teeth belonging to different populations was significantly worse than between those of the same population and that the degree of misfit increased linearly with time since common ancestry, albeit with substantial variance. We used our comparisons to assess the species-level diversity within Batodon, the smallest known placental mammal from the Cretaceous. Our data suggest, with caveats, that instead of belonging to a single species, the specimens assigned to Batodon represent at least two species as different as those belonging to different genera or families of living bats.     

Functional Implications of an Early Exposure to General Anesthesia: Are We Changing the Behavior of Our Children?

There is a rapidly growing body of animal and clinical evidence suggesting that the exposure to anesthetics and sedatives during the critical stages of brain development results in long-lasting (perhaps permanent) impairment in cognitive development in a variety of mammalian species. With improved understanding of the mechanisms responsible for behavioral outcomes of anesthesia-induced developmental neurotoxicity, there is hope for development of protective strategies that will enable safe use of anesthesia in the youngest members of our society. Here, I review presently available evidence regarding anesthesia-induced neurocognitive and social behavioral impairments and possible strategies for preventing them. I also review limited and somewhat controversial evidence that examines the effects of nociception and surgical stimulation on anesthesia-­induced developmental neurotoxicity.     

Using Africa's protected area network to estimate the global population of a threatened and declining species: a case study of the Critically Endangered White‐headed Vulture Trigonoceps occipitalis

The White‐headed Vulture Trigonoceps occipitalis (WhV) is uncommon and largely restricted to protected areas across its range in sub‐Saharan Africa. We used the World Database on Protected Areas to identify protected areas (PAs) likely to contain White‐headed Vultures. Vulture occurrence on road transects in Southern, East, and West Africa was adjusted to nests per km² using data from areas with known numbers of nests and corresponding road transect data. Nest density was used to calculate the number of WhV nests within identified PAs and from there extrapolated to estimate the global population. Across a fragmented range, 400 PAs are estimated to contain 1893 WhV nests. Eastern Africa is estimated to contain 721 nests, Central Africa 548 nests, Southern Africa 468 nests, and West Africa 156 nests. Including immature and nonbreeding birds, and accounting for data deficient PAs, the estimated global population is 5475 ‐ 5493 birds. The identified distribution highlights are alarming: over 78% (n = 313) of identified PAs contain fewer than five nests. A further 17% (n = 68) of PAs contain 5 ‐ 20 nests and 4% (n = 14) of identified PAs are estimated to contain >20 nests. Just 1% (n = 5) of PAs are estimated to contain >40 nests; none is located in West Africa. Whilst ranging behavior of WhVs is currently unknown, 35% of PAs large enough to hold >20 nests are isolated by more than 100 km from other PAs. Spatially discrete and unpredictable mortality events such as poisoning pose major threats to small localized vulture populations and will accelerate ongoing local extinctions. Apart from reducing the threat of poisoning events, conservation actions promoting linkages between protected areas should be pursued. Identifying potential areas for assisted re‐establishment via translocation offers the potential to expand the range of this species and alleviate risk.     

Is the Excessive Use of Microblogs an Internet Addiction? Developing a Scale for Assessing the Excessive Use of Microblogs in Chinese College Students

More and more college students are using microblogs, with some excessive users demonstrating addiction-like symptoms. However, there is currently no published scale available for use in assessing excessive use of these microblogs, a significant impediment to advancing this area of research. We collected data from 3,047 college students in China and developed a Microblog Excessive Use Scale (MEUS) for Chinese college students, comparing it with criteria used for assessing Internet addiction. Our diagnostic scale featured three factors, two of which–“withdrawal and health problem” and “time management and performance”–are already included in Internet addiction assessment scales. The third factor, “social comfort,” does not appear in Internet addiction assessment scales. Our study found that females have significantly higher MEUS scores than males, and that total MEUS scores positively correlated with scores from “self-disclosure” and “real social interaction” scales. These findings differ from results obtained in previous investigations into Internet addiction. Our results indicate that some characteristics of the excessive use of microblogs are different to those of Internet addiction, suggesting that microblog overuse may not correspond exactly to the state of Internet addiction.     

An ESS for the Height of a Plant Population, or an Optimal Height for an Individual?—Rethinking Game-Theoretic Models for Plant Height

Plants only interact with neighbors over restricted distances, so local conditions are of great significance for plants. It is therefore important to consider spatial structure and neighborhood effects if we are to understand plants' strategies. We constructed a spatially-explicit, game theory model to explore optimal height growth at the individual-level. In the model, there is no ESS for height growth at the population level, because there is an “instantaneous” optimal height growth strategy for the individual plant that changes depending on the local light environment. The optimal strategy is plasticity in response to local conditions. Game-theoretic models for plant phenotypic traits should move from “mean-field approximations” towards explicit modeling of local interactions.     

Altered Community Flammability in Florida’s Apalachicola Ravines and Implications for the Persistence of the Endangered Conifer Torreya taxifolia

Plant species and communities often reflect historic fire regimes via ecological and evolutionary responses to recurrent fires. Plant communities of the southeastern USA experience a wide array of fire regimes, perhaps nowhere more marked than the juxtaposition of fire-prone uplands and adjacent mesic ravines along Florida’s Apalachicola River. The ravines contain many endemic and disjunct species, most notably the endangered endemic conifer Torreya taxifolia. A rapid decline in T. taxifolia over the past 60 years has been associated with widespread replacement by other tree species. To understand the changes accompanying the shift in ravine composition, we compared leaf litter flammability of nine historic and contemporary species. We measured maximum flame height, flame duration, smoldering duration, mass loss, absorptive capacity, and drying rate. Ordination and perMANOVA suggest the nine species segregated into three distinct groups: the fire-impeding T. taxifolia and Taxus floridana; an intermediate group of three deciduous angiosperms; and a mixed cluster of four flammable species. Results suggest T. taxifolia and T. floridana were fire-impeding species in these communities, while contemporary dominants burn similarly to the upslope pyric species. The increasing presence of fire-facilitating species may portend a shifting fire regime that further imperils T. taxifolia and other rare species in the formerly fire-safe ravines.     

Are forest‐shrubland mosaics of the Cape Floristic Region an example of alternate stable states?

The idea of alternate stable states (ASS) has been used to explain the juxtaposition of distinct vegetation types within the same climate regime. ASS may explain the co‐existence of relatively inflammable closed‐canopy Afrotemperate forest patches (‘Forest’) within fire‐prone open‐canopy Fynbos in the Cape Floristic Region (CFR) on sandstone‐derived soils. We evaluated the hypothesis that although fire and local topography and hydrology likely determined the paleogeographic boundaries of Forest, present‐day boundaries are additionally imposed by emergent edaphic properties and disturbance histories. We studied vegetation and edaphic properties of Forest‐Transition‐Fynbos vegetation at two sites within the CFR on sandstone‐derived soils and tracked historical change using aerial photography. Whereas Forest and Fynbos have changed little in extent or density since 1945, transition vegetation increased into areas formerly occupied by Fynbos. Forest soils were ubiquitously more nutrient‐rich than Fynbos soils, with transition soils being intermediate. These edaphic differences are not due to geological differences, but instead appear to have emerged as a consequence of different nutrient cycling within the different ecosystems. Soil nutrients are now so different that a switch from Fynbos to Forest is unlikely, in the short term (i.e. decades). Floristically and nutritionally, transitional vegetation is more similar to Fynbos than Forest and may be less resilient to changes in exogenous drivers (e.g. fire). Our findings are consistent with the idea that geologically Forest and Fynbos are largely fire‐derived long‐term ASS, with the stability of each state reinforced by marked soil nutrient differences. In contrast, the intermediate transitional vegetation that might switch states is unlikely to be stable.     

Are patterns of density dependence in the Global Population Dynamics Database driven by uncertainty about population abundance?

Density dependence in population growth rates is of immense importance to ecological theory and application, but is difficult to estimate. The Global Population Dynamics Database (GPDD), one of the largest collections of population time series available, has been extensively used to study cross-taxa patterns in density dependence. A major difficulty with assessing density dependence from time series is that uncertainty in population abundance estimates can cause strong bias in both tests and estimates of strength. We analyse 627 data sets in the GPDD using Gompertz population models and account for uncertainty via the Kalman filter. Results suggest that at least 45% of the time series display density dependence, but that it is weak and difficult to detect for a large fraction. When uncertainty is ignored, magnitude of and evidence for density dependence is strong, illustrating that uncertainty in abundance estimates qualitatively changes conclusions about density dependence drawn from the GPDD.     

Are tyrosine residues involved in the photoconversion of the water‐soluble chlorophyll‐binding protein of Chenopodium album?

Non‐photosynthetic and hydrophilic chlorophyll (Chl) proteins, called water‐soluble Chl‐binding proteins (WSCPs), are distributed in various species of Chenopodiaceae, Amaranthaceae, Polygonaceae and Brassicaceae. Based on their photoconvertibility, WSCPs are categorised into two classes: Class I (photoconvertible) and Class II (non‐photoconvertible). Chenopodium album WSCP (CaWSCP; Class I) is able to convert the chlorin skeleton of Chl a into a bacteriochlorin‐like skeleton under light in the presence of molecular oxygen. Potassium iodide (KI) is a strong inhibitor of the photoconversion. Because KI attacks tyrosine residues in proteins, tyrosine residues in CaWSCP are considered to be important amino acid residues for the photoconversion. Recently, we identified the gene encoding CaWSCP and found that the mature region of CaWSCP contained four tyrosine residues: Tyr13, Tyr14, Tyr87 and Tyr134. To gain insight into the effect of the tyrosine residues on the photoconversion, we constructed 15 mutant proteins (Y13A, Y14A, Y87A, Y134A, Y13‐14A, Y13‐87A, Y13‐134A, Y14‐87A, Y14‐134A, Y87‐134A, Y13‐14‐87A, Y13‐14‐134A, Y13‐87‐134A, Y14‐87‐134A and Y13‐14‐87‐134A) using site‐directed mutagenesis. Amazingly, all the mutant proteins retained not only chlorophyll‐binding activity, but also photoconvertibility. Furthermore, we found that KI strongly inhibited the photoconversion of Y13‐14‐87‐134A. These findings indicated that the four tyrosine residues are not essential for the photoconversion.     

Evaluation of the WHO classification of dengue disease severity during an epidemic in 2011 in the state of Ceará, Brazil

In 2009, the World Health Organization (WHO) issued a new guideline that stratifies dengue-affected patients into severe (SD) and non-severe dengue (NSD) (with or without warning signs). To evaluate the new recommendations, we completed a retrospective cross-sectional study of the dengue haemorrhagic fever (DHF) cases reported during an outbreak in 2011 in northeastern Brazil. We investigated 84 suspected DHF patients, including 45 (53.6%) males and 39 (46.4%) females. The ages of the patients ranged from five-83 years and the median age was 29. According to the DHF/dengue shock syndrome classification, 53 (63.1%) patients were classified as having dengue fever and 31 (36.9%) as having DHF. According to the 2009 WHO classification, 32 (38.1%) patients were grouped as having NSD [4 (4.8%) without warning signs and 28 (33.3%) with warning signs] and 52 (61.9%) as having SD. A better performance of the revised classification in the detection of severe clinical manifestations allows for an improved detection of patients with SD and may reduce deaths. The revised classification will not only facilitate effective screening and patient management, but will also enable the collection of standardised surveillance data for future epidemiological and clinical studies.     

Changes in plant species density in an experimentally fragmented forest landscape: Are the effects scale‐dependent?

The establishment of commercial tree plantations is a common cause of habitat fragmentation globally, yet the influence of this land use on plant species density in fragmented native forests requires further understanding. Theory predicts species density will be highest in large areas of habitat, and should decline as area is reduced, but whether these effects are scale‐dependent is largely unknown. We assessed plant species density (total, common and uncommon species) in experimentally fragmented eucalypt forest remnants (0.25, 0.88, 3.06 ha) surrounded by a pine plantation, at three spatial scales using nested quadrats. Specifically, we consider how plant species density varies across three different sized fragments, and whether the response of species density is magnified in common or uncommon species. Species density in small fragments was higher than continuous forest for all species groupings at the smallest spatial scale (1 m²), and for total and common species at the next smallest spatial scale (16 m²). No species groupings responded to reductions in habitat area at the largest spatial scale (144 m²). We suggest that pine plantations may cause higher species density in small fragments via two mechanisms, either by allowing species to infill unoccupied areas within small fragments, or by buffering small fragments from species losses during a severe and prolonged drought. In both cases we suspect reduced moisture stress (e.g. increased soil moisture, higher shading and reduced temperature) in small fragments has led to the observed changes in species density.     

Establishment of a coastal fish in the Azores: recent colonisation or sudden expansion of an ancient relict population?

The processes and timescales associated with ocean-wide changes in the distribution of marine species have intrigued biologists since Darwin''s earliest insights into biogeography. The Azores, a mid-Atlantic volcanic archipelago located >1000 km off the European continental shelf, offers ideal opportunities to investigate phylogeographic colonisation scenarios. The benthopelagic sparid fish known as the common two-banded seabream (Diplodus vulgaris) is now relatively common along the coastline of the Azores archipelago, but was virtually absent before the 1990s. We employed a multiple genetic marker approach to test whether the successful establishment of the Azorean population derives from a recent colonisation from western continental/island populations or from the demographic explosion of an ancient relict population. Results from nuclear and mtDNA sequences show that all Atlantic and Mediterranean populations belong to the same phylogroup, though microsatellite data indicate significant genetic divergence between the Azorean sample and all other locations, as well as among Macaronesian, western Iberian and Mediterranean regions. The results from Approximate Bayesian Computation indicate that D. vulgaris has likely inhabited the Azores for ∼40 (95% confidence interval (CI): 5.5–83.6) to 52 (95% CI: 6.32–89.0) generations, corresponding to roughly 80–150 years, suggesting near-contemporary colonisation, followed by a more recent demographic expansion that could have been facilitated by changing climate conditions. Moreover, the lack of previous records of this species over the past century, together with the absence of lineage separation and the presence of relatively few private alleles, do not exclude the possibility of an even more recent colonisation event.     

Pair‐living in the Absence of Obligate Biparental Care in a Lizard: Trading‐off Sex and Food?

A pair‐living social organisation can typically be explained by obligate biparental care. We investigated pair‐living in the absence of biparental care in the Australian sleepy lizard, Tiliqua rugosa, which forms exceptionally strong pair bonds. We fitted 10 lizards, five male–female pairs, with Global Positioning System (GPS) recorders and continuously monitored social associations and separations between active pair partners, based on location records taken every 10 min over 3 mo. Males temporarily separated and reunited the pair more frequently than females, but females also contributed to the maintenance of the pair bond. These behavioural data were consistent with the hypothesis that females successfully coerce males into associations with one female. Lower frequencies of social association between pair partners once mating had finished support this interpretation. Males that are coerced into pair associations appear to experience higher costs of pair‐living than females, because males initiated temporary separations of the pair more frequently than females. Males showed higher movement activity and remained active later each day. This sex bias in activity may be an important mechanism to mitigate the higher costs of pair‐living for males. Costs for males might include within‐pair competition for food as females appear more competitive. Our study provides detailed empirical data on a lizard pair bond and provides important insights into pair‐living in the absence of biparental care.     

The peri-urban interface and house infestation with Triatoma infestans in the Argentine Chaco: an underreported process?

Peri-urban infestations with triatomine bugs, their sources and their dynamics haverarely been investigated. Here, we corroborated the reported occurrence ofTriatoma infestans in a peri-urban area and in neighbouring ruralhouses in Pampa del Indio, in the Argentine Chaco, and identified its putativesources using spatial analysis and demographic questionnaires. Peri-urbanhouseholders reported that 10% of their premises had triatomines, whereas T.infestans was collected by timed manual searches or community-basedsurveillance in only nine (3%) houses. Trypanosoma cruzi-infectedT. infestans and Triatoma sordida were collectedindoors only in peri-urban houses and were infected with TcVand TcI, respectively. The triatomines fed on chickens,cats and humans. Peri-urban infestations were most frequent in a squatter settlementand particularly within the recently built mud houses of rural immigrants, withlarge-sized households, more dogs and cats and more crowding. Several of the observedinfestations were most likely associated with passive bug transport from othersources and with active bug dispersal from neighbouring foci. Thus, the households inthe squatter settlement were at a greater risk of bug invasion and colonisation. Insum, the incipient process of domestic colonisation and transmission, along withpersistent rural-to-urban migratory flows and unplanned urbanisation, indicate theneed for active vector surveillance and control actions at the peri-urban interfaceof the Gran Chaco.