We experimentally demonstrate an ultra‐sensitive immunoassay biosensor using diatom biosilica with self‐assembled plasmonic nanoparticles. As the nature‐created photonic crystal structures, diatoms have been adopted to enhance surface plasmon resonances of metal nanoparticles on the surfaces of diatom frustules and to increase the sensitivity of surface‐enhanced Raman scattering (SERS). In this study, a sandwich SERS immunoassay is developed based on the hybrid plasmonic‐biosilica nanostructured materials that are functionalized with goat anti‐mouse IgG. Our experimental results show that diatom frustules improve the detection limit of mouse IgG to 10 pg/mL, which is ?100× better than conventional colloidal SERS sensors on flat glass.
Ultra‐sensitive immunoassay biosensor using diatom biosilica with self‐assembled plasmonic nanoparticles. 相似文献
Photodynamic inactivation of prions by disulfonated hydroxyaluminum phthalocyanine. Further details can be found in the article by Marie Kostelanska, Jaroslav Freisleben, Zdenka Backovska Hanusova, et al. ( e201800430 ).
A STED‐FLIM system is developed to observe the changes of fluorescence lifetime. The pictures show increased lifetime of fluorescent microspheres samples with laser illumination time in both confocal and STED imaging modes. Due to the saturation power of fluorophores is correlated with fluorescence lifetime, the lifetime increase is beneficial for the reduction of the saturation power, indicating the same imaging resolution can be achieved in a lower depletion power. Further details can be found in the article by Lu‐Wei Wang, Yue Chen, Wei Yan, et al. ( e201800315 ).
This schematic depicts the classification of multiphoton images with different degrees of HCC differentiation using the VGG‐16 neural network. The convolution layer is further trained based on the original weights. The weights of the fully connected layers are initialized as a random number and the training is restarted to improve its classification accuracy. Further details can be found in the article by Hongxin Lin, Chao Wei, Guangxing Wang, et al. ( e201800435 ).
How does the ischemic tissue re‐vascularize? Now we can visualize the reperfusion process at high spatial resolution by using a dual‐wavelength MEMS scanning based optical resolution photoacoustic microscopy (OR‐PAM) system. The fast imaging capability enables continuous monitoring of skin reperfusion in a mouse model. It's also found that the ischemic tissue has a significantly higher oxygen consumption rate in the reperfusion stage comparing to the normal tissue. Further details can be found in the article by Renzhe Bi, U.S. Dinish, Chi Ching Goh, et al. ( e201800454 ).
Sub‐picosecond light pulses are used to launch high‐frequency ultrasound in cells. The dual detection of acoustic echoes and of the time‐domain Brillouin scattering allows mapping remotely and in a single run experiment the cell adhesion, thickness, storage modulus and mass density, all with micron resolution. The dual picosecond opto‐acoustic microscope is demonstrated with the multiple imaging of a mitotic macrophage‐like cell. This novel modality is compatible with simultaneous fluorescence imaging. Further details can be found in the article by Liwang Liu, Laurent Plawinski, Marie‐Christine Durrieu, Bertrand Audoin ( e201900045 ).
In this work, intravital multiphoton microscopy was used to image and quantify hepatobiliary metabolism of 6‐carboxyfluorescein diacetate in the recovery of acetaminophen‐overdose mice. It was found that the excretion of the probe molecule was time‐dependent and hepatobiliary metabolism is higher in recovered mice, suggesting that newly regenerated hepatocytes have higher metabolic capabilities. This approach may be further developed applied to studying drug‐induced hepatotoxicity in vivo. Further details can be found in the article by Feng‐Chieh Li, Sheng‐Lin Lee, Hung‐Ming Lin, et al. ( e201800296 ).
Optical tissue clearing is a method allowing post‐mortem deep imaging of organs in three dimensions. By optimizing the CUBIC clearing protocol, the authors provide rapid and simple approach to clear the entire adult rat organism within as little as four days, which is accompanied by the variety of its staining and imaging techniques. The image was captured with polarizers and demonstrates transparent rodent heart with thread‐like crystals of clearing reagent. Further details can be found in the article by Pawe? Matryba et al. ( e201700248 ).
Nuclear pore complex imaged at three different resolutions by confocal, expansion, and Ex‐STED microscopy, respectively. NUP become a ruler to measure the expansion process. Further details can be found in the article by Luca Pesce, Marco Cozzolino, Luca Lanzanò, Alberto Diaspro, and Paolo Bianchini ( e201900018 ).
Cationic gold nanorods stabilized by quaternary ammonium salts (QAS) are a promising tool for photothermal destruction of cancer cells. However, cytotoxicity of the alkanethiol‐QAS limits their medical applications. A novel design of cationic surfactant composed of the quaternary ammonium group and ethylene glycol chain significantly reduces the compound cytotoxicity in the free state while allowing the preparation of stable nanorods with high cellular uptake and lysosomal localization. Further details can be found in the article by Sarka Salajkova, Michal Sramek, David Malinak, et al. ( e201900024 ).
The forward elastic‐light‐scattering pattern of a bacterial colony reflects its morphological characteristics. Three bacteria genera whose colonies having convex, crateriform, or irregular elevation were investigated to study the correlation between the morphology and the scattering pattern of the colony. The difference in the colony elevation produced distinct shapes of light diffraction in the scattering pattern, resulting circular diffraction rings or scattered light. Further details can be found in the article by Iyll‐Joon Doh, Jennifer Sturgis, Diana V. Sarria Zuniga, et al. ( e201900149 ).
Thrombosis monitoring in vivo in small animals is of great value in basic research. The aim of this study is to utilize OCT to monitor thrombosis progression in femoral vein of mice from various measurement criteria, and to validate its use in evaluation the efficacy of the antithrombotic drug. The proved capability of obtaining thrombodynamics information in mice model provide valuable use in preclinical studies for anti‐thrombotic drugs development research. Further details can be found in the article by Yao Yu, Menghan Yu, Jian Liu, et al. ( e201900105 ).
A novel design of an SRS microscope exploiting spectral pulse shaping allows measurement of fingerprint to CH‐stretch SRS spectra without any modification of the optical setup. High spectral resolution over a broad vibrational range allows label‐free quantitative imaging of biological samples. An exemplary SRS broadband spectrum of lipid droplets in a liver cancer cell is shown in the picture. Further details can be found in the article by Sergey P. Laptenok, Vijayakumar P. Rajamanickam, Luca Genchi, et al. ( e201900028 ).
A novel 0.9 mm-diameter intravascular photoacoustic catheter with coaxial excitation and detection was developed to overcome the limitation of imaging range. A miniature ring-shaped ultrasound transducer with a 0.18 mm-diameter orifice in the center was successfully fabricated. The results demonstrated that the coaxial catheter exhibited much better photoacoustic/ultrasound imaging performance from the intima to the adventitia. Further details can be found in the article by Riqiang Lin, Qi Zhang, Shengmiao Lv, Jiaming Zhang, Xiatian Wang, Dongliang Shi, Xiaojing Gong, and Kwok-ho La ( e202200269 ).
Intraoperative margin assessment is clinically important, especially for tissue conserving surgery like Mohs micrographic surgery in which minimization of the surgical area is crucial. Instead of the complex frozen pathology protocol, slide‐free histopathological imaging of hematoxylin‐eosin stained whole‐mount skin tissues is demonstrated by using nonlinear microscopy, thus facilitating rapid intraoperative assessment of surgical tissues for future applications. Further details can be found in the article by Chi‐Kuang Sun, Chien‐Ting Kao, Ming‐Liang Wei, et al. ( e201800341 ).
An ultrafast time‐stretch imaging technique for edge detection is demonstrated. The edge detection based on the optical directional derivative is realized by using differential detection. Since the edge detection is implemented in the physical layer, the computation complexity in the back‐end digital signal processing is very low. The blood red cells and cancer cells can be easily identified by distinguishing the edges. Further details can be found in the article by Bo Dai, LuHe, Lulu Zheng, Yongfeng Fu et al. ( e201800044 ).
A new quantitative phase imaging (QPI) modality, coined multi‐ATOM, can now capture and process enormous amount of quantitative phase single‐cell images (>700,000 cells) at a ultrahigh throughput without compromising sub‐cellular resolution. It could empower label‐free single‐cell analysis where large‐scale and cost‐effective screening is necessary. Further details can be found in the article by Kelvin C. M. Lee, Andy K. S. Lau, Anson H. L. Tang, et al. ( e201800479 ).
Infrared attenuated total reflection spectroscopy is an emerging label‐free method for analyzing the degree of damage in cartilage samples. In the present study, sheep menisci have been characterized after meniscectomy via the variances of relevant biomolecules at the incision surface. Further details can be found in the article by Angela I. López‐Lorente et al. ( e201800429 ).
The newly developed Raman ChemLighter allows the real‐time acquisition of spectroscopic data using a handheld probe. By intelligently combining the fiber‐based imaging approach with computational modeling, we can directly extract molecular information of a sample provide augmented chemical reality to visualize chemistry. Further details can be found in the article by Wei Yang, Abdullah S. Mondol, Clara Stiebing, Laura Marcu, Jürgen Popp, Iwan W. Schie ( e201800447 ).
The tremendous enhancement factors possessed by surfaceenhanced Raman scattering (SERS), coupled with the flexibility of photonic crystal fibers (PCFs), pave the way to a new generation of ultrasensitive biosensors. This review article aims to provide the latest advancement in SERS‐based PCF sensors for various biochemical applications. Such a sensitive biosensor could be translated for the detection of biomarkers in body fluids for early diagnosis of diseases. Further details can be found in the article by U. S Dinish, Flavien Beffara, Georges Humbert, Jean‐Louis Auguste, and Malini Olivo ( e201900027 ).