The estimated life span of Halimeda from a new recruit to a fertile plant. See Mayakun and Prathep in this issue. Cover picture from: Article link here
The tremendous enhancement factors possessed by surfaceenhanced Raman scattering (SERS), coupled with the flexibility of photonic crystal fibers (PCFs), pave the way to a new generation of ultrasensitive biosensors. This review article aims to provide the latest advancement in SERS‐based PCF sensors for various biochemical applications. Such a sensitive biosensor could be translated for the detection of biomarkers in body fluids for early diagnosis of diseases. Further details can be found in the article by U. S Dinish, Flavien Beffara, Georges Humbert, Jean‐Louis Auguste, and Malini Olivo ( e201900027 ).
There is a clinical need in the treatment of atrial fibrillation by radiofrequency ablation to provide lesion set validation. The integration of optical fibers into radiofrequency ablation catheters can address this issue. Thermally‐induced, dynamic changes in tissue diffuse reflectance are measured through transcatheter near‐infrared spectroscopy. A regression model based on spectral features is proposed and utilized to validate the presence of thermal injury, measure lesion depth, and assess gaps. Further details can be found in the article by Rajinder P. Singh‐Moon, Xinwen Yao, Vivek Iyer, et al. ( e201800144 ).
Currently there is no general gold standard to distinguish between Crohn's disease and gastrointestinal luminal tuberculosis tissues, which both have tuberculosis and surrounding fibrous structures. Here we demonstrate that the Mueller matrix derived polarimetric parameters can reveal the structural features of tuberculosis areas and be used as quantitative indicators to distinguish between Crohn's disease and gastrointestinal luminal tuberculosis tissues, which can be useful for the clinical diagnosis. Further details can be found in the article by Teng Liu, Min Lu, Binguo Chen, et al. ( e201900151 ).
Photodynamic inactivation of prions by disulfonated hydroxyaluminum phthalocyanine. Further details can be found in the article by Marie Kostelanska, Jaroslav Freisleben, Zdenka Backovska Hanusova, et al. ( e201800430 ).
Intraoperative margin assessment is clinically important, especially for tissue conserving surgery like Mohs micrographic surgery in which minimization of the surgical area is crucial. Instead of the complex frozen pathology protocol, slide‐free histopathological imaging of hematoxylin‐eosin stained whole‐mount skin tissues is demonstrated by using nonlinear microscopy, thus facilitating rapid intraoperative assessment of surgical tissues for future applications. Further details can be found in the article by Chi‐Kuang Sun, Chien‐Ting Kao, Ming‐Liang Wei, et al. ( e201800341 ).
Optimized light delivery allows for single shot whole organ optoacoustic imaging. The authors present an optimized illumination concept for volumetric tomography that utilizes 3D printing in combination with custom‐made optical fiber illumination. The new approach showed a clear advantage over conventional, single‐sided illumination strategies by eliminating the need to correct for illumination variances and resulting in enhancement of the effective field of view, greater penetration depth and significant improvements in the overall image quality. Further details can be found in the article by Benedict Mc Larney, Johannes Rebling, Zhenyue Chen, et al. ( e201800387 )
This review is aimed at interpreting development and advantages of intravital imaging as an emerging invaluable methodology and summarizing related representative discoveries in bone physiologies and pathologies. It also indicates current limitations, further refinement, and extended application of intravital imaging in bone research. Further details can be found in the article by Yuhao Liu, Quan Yuan, and Shiwen Zhanget ( e201960075 ).
Cover Image A large population (left) and various life history stages (right), e.g. seedling, one-leaf, three-leaved and flowering, of Trillium camschatcense. (cf. Ohara & Kawano, 2005, Plant Species Biology 20:75–82, DOI: 10.1111/j.1442-1984.2005.00126.x ) Photographed by Masashi Ohara, Tokachi, Hokkaido, Japan.
A STED‐FLIM system is developed to observe the changes of fluorescence lifetime. The pictures show increased lifetime of fluorescent microspheres samples with laser illumination time in both confocal and STED imaging modes. Due to the saturation power of fluorophores is correlated with fluorescence lifetime, the lifetime increase is beneficial for the reduction of the saturation power, indicating the same imaging resolution can be achieved in a lower depletion power. Further details can be found in the article by Lu‐Wei Wang, Yue Chen, Wei Yan, et al. ( e201800315 ).
A large‐depth‐of‐field full‐field optical angiography (LD‐FFOA) method is developed to expand the depth‐of‐field (DOF). The contrast pyramid fusion algorithm is used to fuse 10 FFOA images at different focus depth. Cover images of mouse ear shows LD‐FFOA image has higher contrast and more detailed features. The LD‐FFOA method solves the defocused problem caused by the limited DOF of lens, the curved surface and uneven thickness of the sample. Further details can be found in the article by Mingyi Wang, Nanshou Wu, Hongheng Huang, et al. ( e201800329 ).
The forward elastic‐light‐scattering pattern of a bacterial colony reflects its morphological characteristics. Three bacteria genera whose colonies having convex, crateriform, or irregular elevation were investigated to study the correlation between the morphology and the scattering pattern of the colony. The difference in the colony elevation produced distinct shapes of light diffraction in the scattering pattern, resulting circular diffraction rings or scattered light. Further details can be found in the article by Iyll‐Joon Doh, Jennifer Sturgis, Diana V. Sarria Zuniga, et al. ( e201900149 ).
Lens‐free holographic on‐chip microscopy holds tremendous potential to be used for the high‐throughput imaging of pathology slides for clinical diagnosis. However, due to the use of narrowband illumination sources, it is challenging for lens‐free microscopy to achieve a good color accuracy. As a solution to this challenge, an absorbance spectrum estimation‐based colorization (ASEC) method is presented which achieves accurate‐color imaging of pathology slides using only a few (e.g., three) wavelengths. Further details can be found in the article by Yibo Zhang, Tairan Liu, Yujia Huang, et al. ( e201800335 ).
TP‐SAX microscopy images are shown in cyan, cyan hot, red, gray, and orange hot colors; TPFM images are in green color while the LSCM image is in magenta color. Our results show a spatial resolution enhancement for TP‐SAX (cyan image) even at 2.4 mm depth of a mouse brain in comparison with TPFM (green image) where scattering seriously degrades the PSF. Further details can be found in the article by Sandeep Chakraborty, Szu‐Yu Lee, Jye‐Chang Lee, Chen‐Tung Yen, and Chi‐Kuang Sun ( e201800136 ).
A new quantitative phase imaging (QPI) modality, coined multi‐ATOM, can now capture and process enormous amount of quantitative phase single‐cell images (>700,000 cells) at a ultrahigh throughput without compromising sub‐cellular resolution. It could empower label‐free single‐cell analysis where large‐scale and cost‐effective screening is necessary. Further details can be found in the article by Kelvin C. M. Lee, Andy K. S. Lau, Anson H. L. Tang, et al. ( e201800479 ).
The newly developed Raman ChemLighter allows the real‐time acquisition of spectroscopic data using a handheld probe. By intelligently combining the fiber‐based imaging approach with computational modeling, we can directly extract molecular information of a sample provide augmented chemical reality to visualize chemistry. Further details can be found in the article by Wei Yang, Abdullah S. Mondol, Clara Stiebing, Laura Marcu, Jürgen Popp, Iwan W. Schie ( e201800447 ).
This schematic depicts the classification of multiphoton images with different degrees of HCC differentiation using the VGG‐16 neural network. The convolution layer is further trained based on the original weights. The weights of the fully connected layers are initialized as a random number and the training is restarted to improve its classification accuracy. Further details can be found in the article by Hongxin Lin, Chao Wei, Guangxing Wang, et al. ( e201800435 ).
Sub‐picosecond light pulses are used to launch high‐frequency ultrasound in cells. The dual detection of acoustic echoes and of the time‐domain Brillouin scattering allows mapping remotely and in a single run experiment the cell adhesion, thickness, storage modulus and mass density, all with micron resolution. The dual picosecond opto‐acoustic microscope is demonstrated with the multiple imaging of a mitotic macrophage‐like cell. This novel modality is compatible with simultaneous fluorescence imaging. Further details can be found in the article by Liwang Liu, Laurent Plawinski, Marie‐Christine Durrieu, Bertrand Audoin ( e201900045 ).
We experimentally demonstrate an ultra‐sensitive immunoassay biosensor using diatom biosilica with self‐assembled plasmonic nanoparticles. As the nature‐created photonic crystal structures, diatoms have been adopted to enhance surface plasmon resonances of metal nanoparticles on the surfaces of diatom frustules and to increase the sensitivity of surface‐enhanced Raman scattering (SERS). In this study, a sandwich SERS immunoassay is developed based on the hybrid plasmonic‐biosilica nanostructured materials that are functionalized with goat anti‐mouse IgG. Our experimental results show that diatom frustules improve the detection limit of mouse IgG to 10 pg/mL, which is ?100× better than conventional colloidal SERS sensors on flat glass.
Ultra‐sensitive immunoassay biosensor using diatom biosilica with self‐assembled plasmonic nanoparticles. 相似文献
Incorporation of genipin into design of immunoisolation devices is harnessed for in vivo imaging. Using cell‐compatible in situ crosslinking reactions, a fast, efficient and noncytotoxic procedure is shown to maximize fluorescence of microcapsules. By injecting increasing doses of microcapsules in mice, good linearity of signal to microcapsule dose is obtained over several weeks. This allows quantitative assessment of the actual injected dose and monitoring of its position over time, thereby significantly enhancing the efficacy and biosafety of the therapy. Further details can be found in the article by Edorta Santos‐Vizcaino, Henry Haley, Ainhoa Gonzalez‐Pujana, et al. ( e201800170 ).
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