共查询到20条相似文献,搜索用时 390 毫秒
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Map‐based cloning of the pear gene MYB114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis 下载免费PDF全文
Gaifang Yao Meiling Ming Andrew C. Allan Chao Gu Leiting Li Xiao Wu Runze Wang Yaojun Chang Kaijie Qi Shaoling Zhang Jun Wu 《The Plant journal : for cell and molecular biology》2017,92(3):437-451
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Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during otic placode development 下载免费PDF全文
Yu Okamoto Naoko Nishimura Kazunari Matsuda Deshani C. Ranawakage Yusuke Kamachi Hisato Kondoh Masanori Uchikawa 《Development, growth & differentiation》2018,60(3):133-145
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Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells 总被引:3,自引:0,他引:3
Maruyama M Ichisaka T Nakagawa M Yamanaka S 《The Journal of biological chemistry》2005,280(26):24371-24379
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Juan Ouyang Wei Yu Jing Liu Nian Zhang Laurence Florens Jiekai Chen He Liu Michael Washburn Duanqing Pei Ting Xie 《The Journal of biological chemistry》2015,290(37):22782-22794
Sox2 is a key factor in maintaining self-renewal of embryonic stem cells (ESCs) and adult stem cells as well as in reprogramming differentiated cells back into pluripotent or multipotent stem cells. Although previous studies have shown that Sox2 is phosphorylated in human ESCs, the biological significance of Sox2 phosphorylation in ESC maintenance and reprogramming has not been well understood. In this study we have identified new phosphorylation sites on Sox2 and have further demonstrated that Cdk2-mediated Sox2 phosphorylation at Ser-39 and Ser-253 is required for establishing the pluripotent state during reprogramming but is dispensable for ESC maintenance. Mass spectrometry analysis of purified Sox2 protein has identified new phosphorylation sites on two tyrosine and six serine/Threonine residues. Cdk2 physically interacts with Sox2 and phosphorylates Sox2 at Ser-39 and Ser-253 in vitro. Surprisingly, Sox2 phosphorylation at Ser-39 and Ser-253 is dispensable for ESC self-renewal and cell cycle progression. In addition, Sox2 phosphorylation enhances its ability to establish the pluripotent state during reprogramming by working with Oct4 and Klf4. Finally, Cdk2 can also modulate the ability of Oct4, Sox2, and Klf4 in reprogramming fibroblasts back into pluripotent stem cells. Therefore, this study has for the first time demonstrated that Sox2 phosphorylation by Cdk2 promotes the establishment but not the maintenance of the pluripotent state. It might also help explain why the inactivation of CDK inhibitors such as p53, p21, and Arf/Ink4 promotes the induction of pluripotent stem cells. 相似文献
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