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1.

Genome sequences published outside of Standards in Genomic Sciences, March-April 2012

Oranmiyan W. Nelson George M. Garrity 《Standards in genomic sciences》2012,6(2):287-292

The purpose of this table is to provide the community with a citable record of publications of ongoing genome sequencing projects that have led to a publication in the scientific literature. While our goal is to make the list complete, there is no guarantee that we may have omitted one or more publications appearing in this time frame. Readers and authors who wish to have publications added to subsequent versions of this list are invited to provide the bibliographic data for such references to the SIGS editorial office.

Phylum Euryarchaeota

Halococcus hamelinensis, sequence accession PRJNA80845 [1]
“Methanocella conradii” HZ254, sequence accession CP003243 [2]
Thermococcus litoralis NS-C, sequence accession AHVB00000000 [3]

Phylum Crenarchaeota

Candidatus Nitrosopumilus salaria” BD31, sequence accession AEXL00000000 [4]
Candidatus Nitrosoarchaeum limnia, sequence accession AHJG00000000 [5]

Phylum Deinococcus-Thermus

Deinococcus gobiensis, sequence accession CP002536 [6]

Phylum Proteobacteria

Aggregatibacter actinomycetemcomitans strain ANH9381, sequence accession CP003099 [7]
Alishewanella jeotgali, sequence accession AHTH00000000 [8]
Enterobacter aerogenes KCTC 2190, sequence accession CP002824 [9]
Escherichia coli O104:H4, sequence accession AFOB02000092 [10]
Helicobacter pylori strains 17874, sequence accession PRJNA76569 [11]
Helicobacter pylori strains P79, sequence accession PRJNA76567 [11]
Janthinobacterium sp. Strain PAMC 25724, sequence accession AHHB00000000 [12]
Klebsiella oxytoca KCTC 1686, sequence accession CP003218 [13]
Klebsiella pneumoniae subsp. pneumoniae HS11286, sequence accession CP003200 (chromosome), CP003223 (plasmid pKPHS1), CP003224 (plasmid pKPHS2), CP003225 (plasmid pKPHS3), CP003226 (plasmid pKPHS4), CP003227 (plasmid pKPHS5), CP003228 (plasmid pKPHS6) [14]
Oceanimonas sp. GK1, sequence accession CP003171 [15]
“Pseudogulbenkiania ferrooxidans” Strain 2002, sequence accession NZ_ACIS01000000 [16]
Pseudomonas extremaustralis 14-3b, sequence accession AHIP00000000 [17]
Pseudomonas sp. Strain PAMC 25886, sequence accession AHHC00000000 [18]
Psychrobacter, sequence accession AHVZ00000000 [19]
Rahnella sp. Strain Y9602, sequence accession CP002505 [20]
Rhizobium sp. Strain PDO1-076, sequence accession AHZC00000000 [21]
Rhodospirillum photometricum DSM122, sequence accession HE663493 [22]
“Rickettsia sibirica sibirica”, sequence accession AHIZ00000000 [23]
Rickettsia sibirica subsp. mongolitimonae strain HA-91, sequence accession AHZB00000000 [24]
Salmonella enterica subsp. enterica Serotype Enteritidis Strain LA5, sequence accession [25]
Salmonella enterica subsp. enterica Serotype Senftenberg Strain SS209, sequence accession CAGQ00000000 [26]
Salmonella enterica subsp. enterica Serovar Typhi P-stx-12, sequence accession CP003278 (chromosome) and CP003279 (plasmid) [27]
Sphingomonas echinoides ATCC 14820, sequence accession AHIR00000000 [28]
Strain HIMB55, sequence accession AGIF00000000 [29]
Vibrio harveyi CAIM 1792, sequence accession AHHQ00000000 [30]
Wolbachia Strain wAlbB, sequence accession CAGB01000001 to CAGB01000165 [31]
Xanthomonas axonopodis pv. punicae Strain LMG 859, sequence accession CAGJ01000001 to CAGJ01000217 [32]

Phylum Tenericutes

Mycoplasma hyorhinis Strain GDL-1, sequence accession CP003231 [33]

Phylum Firmicutes

Bacillus subtilis, sequence accession BGSCID 3A27 through BGSCID 28A4 [34]
Clostridium difficile Strain CD37, sequence accession AHJJ00000000 [35]
Clostridium perfringens, sequence accession AFES00000000 [36]
Lactobacillus fructivorans KCTC 3543, sequence accession AEQY00000000 [37]
Lactococcus lactis IO-1, sequence accession AP012281 [38]
Lactobacillus plantarum strain NC8, sequence accession AGRI00000000 [39]
Paenibacillus dendritiformis C454, sequence accession AHKH00000000 [40]
Paenibacillus sp. Strain Aloe-11, sequence accession AGFI00000000 [41]
“Peptoniphilus rhinitidis” 1-13^T, sequence accession BAEW01000001 to BAEW01000056 [42]
Streptococcus macedonicus ACA-DC 198, sequence accession HE613569 and HE613570 [43]
Staphylococcus aureus VC40, sequence accession CP003033 [44]
Streptococcus infantarius subsp. infantarius Strain CJ18, sequence accession CP003295 (chromosome), CP003296 (plasmid) [45]
Streptococcus macedonicus ACA-DC 198, sequence accession HE613569 (chromosome), HE613570 (plasmid pSMA198) [46]

Phylum Actinobacteria

Actinoplanes sp. SE50/110, sequence accession CP003170 [47]
Amycolatopsis sp. Strain ATCC 39116, sequence accession [48]
Nocardia cyriacigeorgica GUH-2, sequence accession FO082843 [49]
Salinibacterium sp., sequence accession AHWA00000000 [50]
Streptomyces acidiscabies 84-104, sequence accession AHBF00000000 [51]

Non-Bacterial genomes

Bluetongue Virus Serotype 2, sequence accession AJ783905 (Seg-6) and JQ681257 (Seg-1), JQ681257 (Seg-1), JQ681258 (Seg-2), JQ681259 (Seg-3), JQ681260 (Seg-4), JQ681261 (Seg-5), JQ681262 (Seg-7), JQ6812563 (Seg-8), JQ6812564 (Seg-9), to JQ681265 (Seg-10) [52]
Virus Serotype 1, sequence accession AJ585111 (Seg-2), AJ586659 (Seg-6), JQ282770 (Seg-1), JQ282771 (Seg-3), JQ282772 (Seg-4), JQ282773 (Seg-5), JQ282774 (Seg-7), JQ282775 (Seg-8), JQ282776 (Seg-9), and JQ282777 (Seg-10) [52]
Chloroplast genome of Erycina pusilla, sequence accession JF_746994 [53]
Danio rerio, sequence accession JQ434101 [54]
Enterococcal Bacteriophage SAP6, sequence accession JF731128 [55]
Eubenangee virus, sequence accession JQ070376 through JQ070385 [56]
Fujian/411-like viruses, sequence accession CY087969 to CY088568 [57]
Hantavirus Variant of Rio Mamoré Virus, Maripa Virus, sequence accession JQ611712 (segment S), JQ611713 (segment M), and JQ611714 (segment L) [58]
Pata virus, sequence accession JQ070386 through JQ070395 [59]
Porcine Circovirus 2, sequence accession JQ413808 [60]
Porcine Reproductive and Respiratory Syndrome Virus, sequence accession JQ326271 [61]
Streptococcus mutans Phage M102AD, sequence accession DQ386162 [62]
Tilligery virus, sequence accession JQ070366 through JQ070375 [63]

相似文献

2.

First Report of the Spiral Nematode Rotylenchus incultus (Nematoda: Hoplolaimidae) from Cultivated Olive in Tunisia,with Additional Molecular Data on Rotylenchus eximius

Ilhem Guesmi-Mzoughi Antonio Archidona-Yuste Carolina Cantalapiedra-Navarrete Hajer Regaieg Najet Horrigue-Raouani Juan E. Palomares-Rius Pablo Castillo 《Journal of nematology》2016,48(3):136-138

相似文献

3.

Insights from the GC content analysis of 76genome survey sequences (GSS) from Elaeisoleifera

Bhore SJ Kassim A Shah FH 《Bioinformation》2010,5(4):141-145

South American oil-palm (Elaeis oleifera) is not cultivated in tropical countries like Malaysia on large scale due to low yield of palm oil derived from its fruit mesocarp. However, its fruit mesocarp oil contains about 68.6 % oleic acid (C_18:1) which is more than double in comparison to commercially cultivated oilpalm, E. guineensis Jacq Tenera (hybrid of Dura (♀) x Pisifera (♂)). It is also known that E. oleifera is a good source of tocotrienols and carotenoids. Therefore, it is of interest to know the genome sequence of E. oleifera. The objective of this study is to generate genome survey sequences (GSS) to get GC content insight in the E. oleifera genome. The nuclear genomic DNA isolated from young leaf‐tissues was digested with EcoRI and NdeI/DraI restriction enzymes; and three genomic DNA libraries were constructed using Lambda ZAP‐II, pGEM®‐T Easy, and pDONR 222™ as cloning vectors. Generated 76 GSSs were analyzed by using Bioinformatics tools. The analysis result indicates that the adenine, cytosine, guanine and thymine content in generated GSSs are 30%, 20%, 20%, and 30% respectively. In conclusion, based on the precise GC content analysis of the randomly isolated 76 GSSs by using Bioinformatics tools we hypothesize that GC content in E. oleifera genome is 40%. The hypothesized 40% GC content in E. oleifera genome is expected to remain close to the GC content based on the whole genome analysis.^ψThe nucleotide sequence data reported in this paper have been submitted to dbGSS division of the international DNA database (GenBank/DDBJ/EMBL) under accession numbers: DX575945- DX575972 and EI798032-EI798079.

Abbreviations

gDNA - Nuclear genomic DNA, GSSs - Genome survey sequences K12, SAOP - South American oil‐palm Db1 相似文献

4.

Genome sequences published outside of Standards in Genomic Sciences, January-March 2012

Oranmiyan W. Nelson George M. Garrity 《Standards in genomic sciences》2012,6(1):126-135

The purpose of this table is to provide the community with a citable record of publications of ongoing genome sequencing projects that have led to a publication in the scientific literature. While our goal is to make the list complete, there is no guarantee that we may have omitted one or more publications appearing in this time frame. Readers and authors who wish to have publications added to subsequent versions of this list are invited to provide the bibliographic data for such references to the SIGS editorial office.

Phylum Crenarchaeota

Pyrobaculum strain 1860, sequence accession CP003098 [1]

Phylum Deinococcus-Thermus

“Thermus sp.” Strain CCB_US3_UF1, sequence accession CP003126 (chromosome), CP003127 (plasmid) [2]

Phylum Proteobacteria

“Achromobacter arsenitoxydans” SY8, sequence accession AGUF00000000 [3]
Acidovorax sp. Strain NO1, sequence accession AGTS00000000 [4]
Acinetobacter baumannii AB4857, sequence accession AHAG00000000 [5]
Acinetobacter baumannii AB5075, sequence accession AHAH00000000 [5]
Acinetobacter baumannii AB5256, sequence accession AHAI00000000 [5]
Acinetobacter baumannii AB5711, sequence accession AHAJ00000000 [5]
Aeromonas salmonicida, sequence accession AGVO00000000 [6]
Aggregatibacter actinomycetemcomitans RHAA1, sequence accession AHGR00000000 [7]
Agrobacterium tumefaciens 5A, sequence accession AGVZ00000000 [8]
Azoarcus sp. Strain KH32C, sequence accession AP012304, AP012305 [9]
Burkholderia sp. Strain YI23, sequence accession CP003087 (Chromosome 1), CP003088 (Chromosome 2), CP003089 (Chromosome 3), CP003090 (plasmid BYI23_D), CP003091 (plasmid BYI23_E) CP003092 (plasmid BYI23_F) [10]
Brucella suis VBI22, sequence accession CP003128, CP003129 [11]
Comamonas testosteroni ATCC 11996, sequence accession AHIL00000000 [12]
“Commensalibacter intestini” A911^T, sequence accession AGFR00000000 [13]
Edwardsiella ictaluri, sequence accession CP001600.1 [14]
Enterobacter cloacae subsp. dissolvens SDM, sequence accession AGSY00000000 [15]
“Gluconobacter morbifer” G707^T, sequence accession AGQV00000000 [16]
Legionella dumoffii TEX-KL, sequence accession AGVT00000000 [17]
Legionella dumoffii NY-23, sequence accession AGVU00000000 [17]
Legionella pneumophila serogroup 12 Strain 570-CO-H, sequence accession CP003192 [18]
Marinobacterium stanieri S30, sequence accession AFPL00000000 [19]
“Marinobacter manganoxydans” MnI7-9, sequence accession CP001978 to CP001980 [20]
Mesorhizobium alhagi CCNWXJ12-2T, sequence accession AHAM00000000 [21]
Mesorhizobium amorphae, sequence accession AGSN00000000 [22]
Methylomicrobium alcaliphilum 20Z, sequence accession FO082060 and FO082061 [23]
Mitsuaria sp. Strain H24L5A, sequence accession CAFG01000001 to CAFG01000607 [24]
Novosphingobium pentaromativorans US6-1, sequence accession AGFM00000000 [25]
Pantoea ananatis B1-9, sequence accession CAEI01000001 to CAEI01000169 [26]
Pantoea ananatis LMG 5342, sequence accession HE617160 (chromosome), HE617161 (pPANA10) [27]
Pantoea ananatis Strain PA13, sequence accession CP003085 and CP003086 [28]
Pseudomonas aeruginosa, sequence accession AFXI00000000 [29]
Pseudomonas aeruginosa, sequence accession AFXJ00000000 [29]
Pseudomonas aeruginosa, sequence accession AFXK00000000 [29]
Pseudomonas chlororaphis GP72, sequence accession AHAY01000000 [30]
Pseudomonas fluorescens F113, sequence accession CP003150 [31]
Pseudomonas fluorescens Wayne 1R, sequence accession CADX01000001 to CADX01000090 [32]
Pseudomonas fluorescens Wood1R, sequence accession CAFF01000001 to CAFF01001437 [32]
Pseudomonas psychrotolerans L19, sequence accession AHBD00000000 [33]
Pseudoalteromonas rubra ATCC 29570^T, sequence accession AHCD00000000 [34]
Pseudomonas stutzeri SDM-LAC, sequence accession AGSX00000000 [35]
Pseudoxanthomonas spadix BD-a59, sequence accession CP003093 [36]
Rickettsia slovaca, sequence accession CP002428 [37]
Salmonella enterica serovar Pullorum RKS5078, sequence accession CP003047 [38]
Sinorhizobium meliloti CCNWSX0020, sequence accession AGVV00000000 [39]
Sphingobium sp. Strain SYK-6, sequence accession AP012222 and AP012223 [40]
Sphingomonas sp. Strain PAMC 26605, sequence accession AHIS00000000 [41]
Stenotrophomonas maltophilia RR-10, sequence accession AGRB00000000 [42]
Strain HIMB30, sequence accession AGIG00000000 [43]
Taylorella equigenitalis, sequence accession CP003059 [44]
Vibrio campbellii DS40M4, sequence accession AGIE00000000 [45]
Vibrio fischeri SR5, sequence accession AHIH00000000 [46]
Yersinia enterocolitica, sequence accession AGQO00000000 [47]

Phylum Tenericutes

Candidatus Mycoplasma haemominutum, sequence accession HE613254 [48]
Mycoplasma haemocanis strain Illinois, sequence accession CP003199 [49]
Mycoplasma iowae, sequence accession AGFP00000000 [50]
Mycoplasma pneumoniae Type 2a Strain 309, sequence accession AP012303 [51]

Phylum Firmicutes

Bacillus cereus F837/76, sequence accession CP003187 (chromosome) CP003188 (pF837_55kb), CP003189 (pF837_10kb) [52]
Brevibacillus laterosporus Strain GI-9, sequence accession CAGD01000001 to CAGD01000061 [53]
Clostridium sporogenes PA 3679, sequence accession AGAH00000000 [54]
Enterococcus mundtii CRL1656, sequence accession AFWZ00000000.1 [55]
Geobacillus thermoleovorans CCB_US3_UF5, sequence accession CP003125 [56]
Lactobacillus curvatus Strain CRL705, sequence accession AGBU01000000 [57]
Lactobacillus rhamnosus ATCC 8530, sequence accession CP003094 [58]
Lactobacillus rhamnosus R0011, sequence accession AGKC00000000 [59]
Lactococcus garvieae TB25, sequence accession AGQX01000000 [60]
Lactococcus garvieae LG9, sequence accession AGQY01000000 [60]
Lactococcus lactis subsp. cremoris A76, sequence accession CP003132 (chromosome), CP003136 (pQA505), CP003135 (PQA518), CP003134 (pQA549), CP003133 (pQA554) [61]
Leuconostoc citreum LBAE C10, sequence accession CAGE00000000 [62]
Leuconostoc citreum LBAE C11, sequence accession CAGF00000000 [62]
Leuconostoc citreum LBAE E16, sequence accession CAGG00000000 [62]
Leuconostoc mesenteroides subsp. mesenteroides Strain J18, sequence accession CP003101 [63]
Paenibacillus peoriae Strain KCTC 3763^T, sequence accession AGFX00000000 [64]
Pediococcus acidilactici MA18/5M, sequence accession AGKB00000000 [65]
Pediococcus claussenii ATCC BAA-344^T, sequence accession CP003137 (chromosome), CP003138 (pPECL-1), CP003139 (pPECL-2), CP003140 (pPECL-3), CP003141 (pPECL-4), CP003142 (pPECL-5), CP003143 (pPECL-6), CP003144 (pPECL-7), CP003145 (pPECL-8) [66]
Staphylococcus aureus M013, sequence accession CP003166 [67]
Staphylococcus aureus subsp. aureus TW20, sequence accession FN433596 [68]
Weissella confusa LBAE C39-2, sequence accession CAGH00000000 [69]

Phylum Actinobacteria

Corynebacterium casei, sequence accession CAFW01000001 to CAFW01000106 [70]
Corynebacterium glutamicum, sequence accession AGQQ00000000 [71]
Leucobacter chromiiresistens, sequence accession AGCW00000000 [72]
Mycobacterium abscessus, sequence accession AGQU00000000 [73]
Propionibacterium acnes ST9, sequence accession CP003195 [74]
Propionibacterium acnes ST22, sequence accession CP003196 [74]
Propionibacterium acnes ST27, sequence accession CP003197 [74]
Saccharomonospora azurea SZMC 14600, sequence accession AHBX00000000 [75]
Streptomyces sp. Strain TOR3209, sequence accession AGNH00000000 [76]
Streptomyces sp. Strain W007, sequence accession AGSW00000000 [77]

Phylum Spirochaetes

Borrelia valaisiana VS116, sequence accession ABCY02000001 (chromosome), CP001439 (plasmid Ip17), CP001437 (Ip25), CP001440 (plasmid Ip 28-3), CP001442 (plasmid Ip28-8), CP001436 (Ip 36), CP001433 (plasmid Ip 54), CP001438 (plasmid cp9), CP001432 (plasmid cp26), CP001441 (plasmid cp32-5), CP001434 (plasmid cp32-7), CP001435 (plasmid cp32-10) [78]
“Borrelia bissettii” DN127, sequence accession CP002746 (chromosome), CP002756 (plasmid Ip12), CP002757 (plasmid Ip25), CP002758 (plasmid 28-3), CP002759 (plasmid Ip 28-4), CP002760 (Ip28-7), CP002761 (plasmid Ip54), CP002762 (plasmid Ip56), CP002755 (plasmid cp9), CP002747 (plasmid cp26), CP002749 (plasmid cp32-3), CP002750 (plasmid cp32-4), CP002751 (plasmid 32-5), CP002752 (plasmid cp32-6), CP002753 (plasmid cp32-7), CP0027554 (plasmid cp32-9), CP002748 (plasmid cp32-11) [78]
Borrelia spielmanii A14S, sequence accession ABKB02000001 (chromosome), CP001468 (plasmid Ip17), CP001471 (Ip28-3), CP001470 (plasmid Ip28-4), CP001465 (plasmid Ip28-2), CP001466 (plasmid Ip36), CP001464 (plasmid Ip38), CP001469 (plasmid Ip54), ABKB02000016, ABKB02000020 (plasmid cp9), CP001467 (plasmid cp26), ABKB02000026 (plasmid cp32-3), ABKB02000031 (plasmid 32-5), ABKB02000021 (plasmid cp32-12), ABKB02000014 (unidentified) [78]

Non-Bacterial genomes

Aspergillus flavus, sequence accession GSE32177 [79]
Bacteriophage SPN3UB, sequence accession JQ288021 [80]
Bamboo mitochondria, sequence accession JQ235166 to JQ235179 [81]
Boea hygrometrica chloroplast, sequence accession JN107811 [82]
Boea hygrometrica mitochondrial, sequence accession JN107812 [82]
Canine Picornavirus, sequence accession JN831356 [83]
Chandipura virus (CHPV) CIN0327, sequence accession GU212856.1 [84]
Chandipura virus (CHPV) CIN0451, sequence accession GU212857.1 [84]
Chandipura virus (CHPV) CIN0751, sequence accession GU212858.1 [84]
Chandipura virus (CHPV) CIN0755, sequence accession GU190711.1 [84]
Chinese Porcine Parvovirus Strain PPV2010, sequence accession JN872448 [85]
Common midwife toad megavirus, sequence accession JQ231222 [86]
Dengue Virus Serotype 4, sequence accession JN983813 [87]
Duck Tembusu Virus, sequence accession JF270480 [88]
Duck Tembusu Virus, sequence accession JQ314464 [88]
Duck Tembusu Virus, sequence accession JQ314465 [88]
Emiliania huxleyi Virus 202, sequence accession HQ634145 [89]
Emiliania huxleyi Virus EhV-88, sequence accession JF974310 [89]
Emiliania huxleyi EhV-201, sequence accession JF974311 [89]
Emiliania huxleyi EhV-207, sequence accession JF974317 [89]
Emiliania huxleyi EhV-208, sequence accession JF974318 [89]
Glarea lozoyensis, sequence accession GUE00000000 [90]
Nannochloropis gaditana, sequence accession AGNI00000000 [91]
Oryza sativa cv., sequence accession DRA000499 [92]
Partetravirus, sequence accession JN990269 [93]
Porcine Bocavirus PBoV5, sequence accession JN831651 [94]
Porcine epidemic diarrhea virus, sequence accession JQ282909 [95]
Pseudomonas aeruginosa lytic bacteriophage PA1Ø, sequence accession HM624080 [96]
Pseudomonas fluorescens phage OBP, sequence accesssion JN627160 [97]
RNA Virus from Avocado, sequence accession JN880414 [98]
Salmonella enterica Serovar Typhimurium Bacteriophage SPN1S, sequence accession JN391180 [99]
Schistosoma haematobium, sequence accession PRJNA78265 [100]
Schistosoma mansoni, sequence accession ERP00038 [101]
Stenopirates sp., sequence accession JN100019 [102]
T7-Like Virus, sequence accession JN651747 [103]
Vibrio harveyi siphophage VHS1, sequence accession JF713456 [104]
Tyrolean ice man, sequence accession ERP001144 [105]

相似文献

5.

Investigating the Role of the Melanocortin-1 Receptor Gene in an Extreme Case of Microgeographical Variation in the Pattern of Melanin-Based Plumage Pigmentation

Yann X. C. Bourgeois Joris A. M. Bertrand Christophe Thébaud Borja Milá 《PloS one》2012,7(12)

The Réunion grey white-eye (Zosterops borbonicus) is a single-island endemic passerine bird that exhibits striking geographically structured melanic polymorphism at a very small spatial scale. We investigated the genetic basis of this color polymorphism by testing whether the melanocortin-1 receptor (MC1R), a gene often involved in natural melanic polymorphism in birds, was associated with the observed plumage variation. Although we found three non-synonymous mutations, we detected no association between MC1R variants and color morphs, and the main amino-acid variant found in the Réunion grey white-eye was also present at high frequency in the Mauritius grey white-eye (Zosterops mauritianus), its sister species which shows no melanic polymorphism. In addition, neutrality tests and analysis of population structure did not reveal any obvious pattern of positive or balancing selection acting on MC1R. Altogether these results indicate that MC1R does not play a role in explaining the melanic variation observed in the Réunion grey white-eye. We propose that other genes such as POMC, Agouti or any other genes involved in pigment synthesis will need to be investigated in future studies if we are to understand how selection shapes complex patterns of melanin-based plumage pigmentation.

Trial Registration

All sequences submitted to Genbank. Accession number: JX914505 to JX914564. 相似文献

6.

First Report of Longidorus kuiperi and Rotylenchus eximius from Coastal Sand Dunes in Crete,Greece

Emmanuel A. Tzortzakakis Carolina Cantalapiedra-Navarrete Antonio Archidona-Yuste Juan E. Palomares-Rius Pablo Castillo 《Journal of nematology》2016,48(3):135

相似文献

7.

Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-Lyase Gene from Dendrobium

Qing Jin Yao Yao Yongping Cai Yi Lin 《PloS one》2013,8(4)

In this study, a phenylalanine ammonia-lyase (PAL) gene was cloned from Dendrobium candidum using homology cloning and RACE. The full-length sequence and catalytic active sites that appear in PAL proteins of Arabidopsis thaliana and Nicotiana tabacum are also found: PAL cDNA of D. candidum (designated Dc-PAL1, GenBank No. JQ765748) has 2,458 bps and contains a complete open reading frame (ORF) of 2,142 bps, which encodes 713 amino acid residues. The amino acid sequence of DcPAL1 has more than 80% sequence identity with the PAL genes of other plants, as indicated by multiple alignments. The dominant sites and catalytic active sites, which are similar to that showing in PAL proteins of Arabidopsis thaliana and Nicotiana tabacum, are also found in DcPAL1. Phylogenetic tree analysis revealed that DcPAL is more closely related to PALs from orchidaceae plants than to those of other plants. The differential expression patterns of PAL in protocorm-like body, leaf, stem, and root, suggest that the PAL gene performs multiple physiological functions in Dendrobium candidum. 相似文献

8.

Hydrolyzing Proficiency of Keratinases in Feather Degradation

Sonali Gupta Rajni Singh 《Indian journal of microbiology》2014,54(4):466-470

The keratinase degrade highly rigid, cross linked structural polypeptides with different efficiency depending on the type of source. Two newly isolated strains of Bacillus subtilis (RSE163 and RSE165; NCBI Accession no JQ887983 and JQ887982) were found to be efficient keratinase producers with unusual catalytic activity result in different morphological changes in degradation pattern of feather, confirmed by their scanned electron micrographs. Maximum keratinolytic activity of both the strains B. subtilis RSE163 and RSE165 were found to be 366 ± 15.79 and 194 ± 7.26 U after 72 h of incubation. While the disulphide reductase activity of RSE163 and RSE165 estimated 0.24 ± 0.05 and 0.15 ± 0.03 U/ml of enzyme after 24 h of incubation. A total of 16 free amino acids of variable concentration were also analyzed in the cell free supernatant of hydrolyzed feather from two strains. Present study demonstrates the action of two different keratinases in feather degradation.

Electronic supplementary material

The online version of this article (doi:10.1007/s12088-014-0477-5) contains supplementary material, which is available to authorized users. 相似文献

9.

HIV-2 Genetic Evolution in Patients with Advanced Disease Is Faster than That in Matched HIV-1 Patients

Helena Skar Pedro Borrego Timothy C. Wallstrom Mattias Mild José Maria Marcelino Helena Barroso Nuno Taveira Thomas Leitner Jan Albert 《Journal of virology》2010,84(14):7412-7415

相似文献

10.

Gábor Reuter Péter Pankovics Nick J. Knowles ákos Boros 《Journal of virology》2012,86(24):13295-13302

Two novel picornaviruses were serendipitously identified in apparently healthy young domestic animals—cattle (Bos taurus) and, subsequently, sheep (Ovis aries)—in Hungary during 2008 and 2009. Complete genome sequencing and comparative analysis showed that the two viruses are related to each other and have identical genome organizations, VPg + 5′ UTR^IRES-II[L/1A-1B-1C-1D-2A^NPG↓P/2B-2C/3A-3B^VPg-3C^pro-3D^pol] 3′ UTR-poly(A). We suggest that they form two novel viral genotypes/serotypes, bovine hungarovirus 1 (BHuV-1; GenBank accession number JQ941880) and ovine hungarovirus 1 (OHuV-1; GenBank accession number HM153767), which may belong to a potential novel picornavirus genus in the family Picornaviridae. The genome lengths of BHuV-1 and OHuV-1 are 7,583 and 7,588 nucleotides, each comprising a single open reading frame encoding 2,243 and 2,252 amino acids, respectively. In the 5′ untranslated regions (5′ UTRs), both hungaroviruses are predicted to have a type II internal ribosome entry site (IRES). The nucleotide sequence and the secondary RNA structure of the hungarovirus IRES core domains H-I-J-K-L are highly similar to that of human parechovirus (HPeV) (genus Parechovirus), especially HPeV-3. However, in the polyprotein coding region, the amino acid sequences are more closely related to those of porcine teschoviruses (genus Teschovirus). Hungaroviruses were detected in 15% (4/26) and 25% (4/16) of the fecal samples from cattle and sheep, respectively. This report describes the discovery of two novel picornaviruses in farm animals, cattle and sheep. The mosaic genetic pattern raises the possibility that hungaroviruses, human parechoviruses, and porcine teschoviruses may be linked to each other by modular recombination of functional noncoding RNA elements. 相似文献

11.

Genome sequences of Bacteria and Archaea published outside of Standards in Genomic Sciences, June �C September 2011

Oranmiyan W. Nelson George M. Garrity 《Standards in genomic sciences》2011,5(1):154-167

The purpose of this table is to provide the community with a citable record of publications of ongoing genome sequencing projects that have led to a publication in the scientific literature. While our goal is to make the list complete, there is no guarantee that we may have omitted one or more publications appearing in this time frame. Readers and authors who wish to have publications added to this subsequent versions of this list are invited to provide the bibliometric data for such references to the SIGS editorial office.

Phylum Crenarchaeota
Phylum Euryarchaeota
Pyrococcus yayanosii CH1, sequence accession CP002779 [1]
Methanocella paludicola, sequence accession AP011532 [2]
Halorhabdus tiamatea, sequence accession AFNT00000000 [3]
Thermococcus sp. Strain 4557, sequence accession CP002920 [4]
Phylum Chloroflexi
Phylum Proteobacteria
Ralstonia solanacearum strain Po82, sequence accession CP002819 (chromosome) and CP002820 (megaplasmid) [5
Desulfovibrio alaskensis G20, sequence accession CP000112 [6]
Methylophaga aminisulfidivorans MP^T, sequence accession AFIG00000000 [7]
Acinetobacter sp. P8-3-8, sequence accession AFIE00000000 [8]
Sphingomonas strain KC8, sequence accession AFMP01000000 [9]
Brucella pinnipedialis B2/94, sequence accession CP002078 and CP002079 [10]
Salmonella enterica Serovar Typhimurium UK-1, sequence accession CP002614 (chromosome), CP002615 (plasmid) [11]
Bordetella pertussis CS, sequence accession CP002695 [12]
Alteromonas sp. Strain SN2, sequence accession CP002339 [13]
Escherichia coli O104:H4, sequence accession AFOB00000000 (LB226692) and AFPS00000000 (01-09591) [14]
Acidithiobacillus caldus, sequence accession CP002573 (Chromosome), CP002574 (pLAtcm), CP002575 (pLAtc1), CP002576 (pLAtc2), CP002577 (pLAtc3) [15]
Cupriavidus necator N-1, sequence accession CP002877 (chromosome 1), CP002878 (chromosome 2), CP002879 (pBB1), and CP002880 (pBB2) [16]
Oligotropha carboxidovorans OM4, sequence accession CP002821 (OM4 chromosome), CP002822 (pHCG3b), CP002823 (pOC167B) [17]
Oligotropha carboxidovorans OM5, sequence accession CP002826 (OM5 chromosome), CP002827 (pHCG3), and CP002828 (pOC167) [17]
Pantoea ananatis LMG20103, sequence accession CP001875 [18]
Helicobacter bizzozeronii strain CIII-1, sequence accession FR871757 (chromosome) and FR871758 (HBZ-1) [19]
Vibrio anguillarum 775, sequence accession CP002284 to CP002285 [20]
Zymomonas mobilis subsp. pomaceae, sequence accession CP002865 (chromosome), CP002866 (p29192_1), CP002867 (p29192_2) [21]
Agrobacterium sp. strain ATCC 31749, sequence accession AECL01000000 [22]
Xanthomonas spp. strain Xrc, sequence accesssion CP002789 [23]
Xanthomonas spp. strain Xoc, sequence accesssion AAQN00000000 [23]
Glaciecola sp. Strain 4H-3-7+YE-5, sequence accession CP002526 (chromosome) and CP002527 (plasmid) [24]
Escherichia coli Strain HM605, sequence accession CADZ01000001 through CADZ01000154 [25]
Salinisphaera shabanensis, sequence accession AFNV00000000 [26]
Methyloversatilis universalis FAM5^T, sequence accession AFHG00000000 [27]
Alicycliphilus denitrificans Strain BC, sequence accession CP002449 (chromosome), CP002450 (megaplasmid), CP002451 (plasmid) [28].
Alicycliphilus denitrificans K601^T, sequence accession CP002657 (chromosome) and CP002658 (plasmid) [28]
Oligotropha carboxidovorans Strain OM4, sequence accession CP002821 (chromosome), CP002822 (pHCG3b), CP002823 (pOC167B) [29]
Oligotropha carboxidovorans Strain OM5, sequence accession CP002826 (chromosome), CP002827 (pHCG3), and CP002828 (pOC167) [29]
Bradyrhizobiaceae strain SG-6C, sequence accession AFOF01000000 [30]
Hyphomicrobium sp. Strain MC1, sequence accession FQ859181 [31]
Shewanella sp. Strain HN-41, sequence accession AFOZ01000000 [32]
Myxococcus fulvus HW-1, sequence accession CP002830 [33]
Nitrosomonas sp. Strain AL212, sequence accession NC_015222 (chromosome), NC_015223 pNAL21201), NC_015221 (pNAL21202) [34]
Ruegeria sp. Strain KLH11, sequence accession ACCW00000000 [35]
Acidovorax avenae subsp. avenae RS-1, sequence accession AFPT01000000 [36]
Escherichia coli (ExPEC), sequence accession AFAT00000000 [37]
Vibrio mimicus SX-4, sequence accession ADOO01000000 [38]
Agrobacterium tumefaciens Strain F2, sequence accession AFSD00000000 [39]
Pasteurella multocida subsp. gallicida AFRR01000001 to AFRR01000489 [40]
Pseudomonas aeruginosa 138244, sequence accession AEVV00000000 [41]
Pseudomonas aeruginosa 152504, sequence accession AEVW00000000 [41]
Campylobacter jejuni strain 305, sequence accession ADHL00000000 [42]
Campylobacter jejuni strain DFVF1099, sequence accession ADHK00000000 [42]
Xanthomonas campestris pv. raphani strain 756C, sequence accession CP002789 [43]
Xanthomonas campestris pv. raphani strain BLS256, sequence accession AAQN01000001 [43]
Rickettsia heilongjiangensis, sequence accession CP002912 [44]
Acidiphilium sp. Strain PM (DSM 24941), sequence accession AFPR00000000 [45]
Pseudomonas putida Strain S16, sequence accession CP002870 [46]
Acinetobacter lwoffii, sequence accession AFQY01000000 [47]
Phylum Firmicutes
Caldalkalibacillus thermarum strain TA2.A1, sequence accession AFCE00000000 [48]
Listeria monocytogenes Scott A, sequence accession AFGI00000000 [49]
Lactococcus garvieae 8831, sequence accession AFCD00000000 [50]
Natranaerobius thermophilus JW/NM-WN-LF, sequence accession CP001034 (chromosome), CP001035 (plasmid) [51]
Melissococcus plutonius ATCC 35311, sequence accession AP012200 (chromosome) and AP012201 (plasmid) [52]
Lactobacillus buchneri NRRL B-30929, sequence accession CP002652 (chromosome), CP002653 (plasmid pLBU01), CP002654 (plasmid pLBU02), and CP002655 (plasmid pLBU03) [53]
Lactobacillus kefiranofaciens ZW3 , sequence accession CP002764 (chromosome), CP002765 (plasmid), and CP002766 (plasmid) [54]
Bacillus megaterium strain QM B1551, sequence accession CP001983 (chromosome), CP001984 to CP001990 (plasmids pBM100 through pBM700) [55]
Bacillus megaterium strain DSM319, sequence accession CP001982 (chromosome) [55]
Listeria monocytogenes serovar 4a strain M7, sequence accession CP002816 [56]
Bacillus coagulans 2-6, sequence accession CP002472 [57]
Streptococcus salivarius strain CCHSS3, sequence accession FR873481 [58]
Paenibacillus elgii B69, sequence accession AFHW01000000 [59]
Lactobacillus pentosus MP-10, sequence accession FR871759 through FR871848 [60]
Leuconostoc pseudomesenteroides KCTC 3652, sequence accession AEOQ00000001 through AEOQ00001160 [61]
Lactobacillus mali KCTC 3596, sequence accession BACP01000001 through BACP01000122 [62]
Paenibacillus polymyxa Type Strain ATCC 842^T, sequence accession AFOX01000000 [63]
Streptococcus salivarius strain JIM8777, sequence accssion FR873482 [64]
Lactobacillus cypricasei KCTC 13900, sequence accession BACS01000001 to BACS01000487 [65]
Lactobacillus zeae KCTC 3804, sequence accession BACQ01000001 to BACQ101000113 [66]
Listeria monocytogenes Serovar 4a Strain M7, sequence accession CP002816 [67]
Lactobacillus salivarius GJ-24, sequence accession AFOI00000000 [68]
Lactobacillus johnsonii PF01, sequence accession AFQJ01000000 [69]
Clostridium acetobutylicum DSM 1731, sequence accession CP002660 through CP002662 [70]
Lactobacillus suebicus KCTC 3549, sequence accession BACO01000000 [71]
Brevibacillus laterosporus LMG 15441, sequence accession AFRV00000000 [72]
Lactobacillus salivarius NIAS840, sequence accession AFMN00000000 [73]
Bifidobacterium animalis subsp. lactis CNCM I-2494, sequence accession CP002915 [74]
Megasphaera elsdenii, sequence accession HE576794 [75]
Lactobacillus versmoldensis KCTC 3814, sequence accession BACR01000001 to BACR01000102 [76]
Lactobacillus pentosus IG1, sequence accession FR874848 to FR874860 [77]
Alicyclobacillus acidocaldarius Strain Tc-4-1, sequence accession CP002902 [78]
Streptococcus thermophilus Strain JIM8232, sequence accession FR875178 [79]
Streptococcus equi subsp. zooepidemicus Strain ATCC 35246, sequence accession CP002904 [80]
Bacillus amyloliquefaciens XH7, sequence accession CP002927 [81]
Leuconostoc kimchii Strain C2, sequence accession CP002898 [82]
Lactobacillus malefermentans KCTC 3548, sequence accession BACN01000001 to BACN01000172 [83]
Weissella koreensis KACC 15510, sequence accession CP002900 [84]
Phylum Tenericutes
Mycoplasma bovis Strain Hubei-1, sequence accession CP002513 [85]
Mycoplasma fermentans Strain M64, sequence accession NC_014921 [86]
Haloplasma contractile, sequence accession AFNU00000000 [87]
Mycoplasma ovipneumoniae Strain SC01, sequence accession AFHO01000000 [88]
Phylum Actinobacteria
Kocuria rhizophila P7-4, sequence accession AFID00000000 [89]
Streptomyces S4, sequence accession CADY01000000 [90]
Corynebacterium nuruki S6-4^T, sequence accession AFIZ00000000 [91]
Propionibacterium humerusii, sequence accession AFAM00000000.1 [92]
Strain JDM601, sequence accession CP002329 [93]
Streptomyces sp. strain Tü6071, sequence accession AFHJ01000000 [94]
Bifidobacterium breve UCC2003, sequence accession CP000303 [95]
Propionibacterium acnes, sequence accession CP002815 [96]
Amycolicicoccus subflavus DQS3-9A1^T, sequence accession CP002786 (chromosome), CP002787 (plasmid pAS9A-1), and CP002788 (plasmid pAS9A-2). [97]
Gordonia neofelifaecis NRRL B-59395, sequence accession AEUD01000000 [98]
Pseudonocardia dioxanivorans strain CB1190, sequence accession NC_015312-4 and CP002595-7 [99]
Bifidobacterium longum subsp. longum KACC 91563, sequence accession CP002794 to CP002796 [100]
Streptomyces cattleya NRRL 8057, sequence accession FQ859185 (chromosome) and FQ859184 (megaplasmid) [101]
Rhodococcus sp. Strain R04, sequence accession AFAQ01000000 [102]
Mycobacterium bovis BCG Moreau, sequence accession [103]
Saccharopolyspora spinosa NRRL 18395, sequence accession [104]
Mycobacterium tuberculosis CCDC5079, sequence accession [105]
Mycobacterium tuberculosis CCDC5180, sequence accession [105]
Amycolatopsis mediterranei S699, sequence accession CP002896 [106]
Nesterenkonia sp. Strain F, sequence accession AFRW01000000 [107]
Streptomyces xinghaiensis NRRL B24674^T, sequence accession AFRP01000000 [108]
Phylum Chlamydiae
Chlamydophila abortus variant strain LLG, sequence accession AFHM01000000 [109]
Chlamydia psittaci 6BC, sequence accession CP002586 (chromosome), CP002587 (plasmid) [110]
Chlamydia psittaci Cal10, sequence accession AEZD00000000 (draft chromosome and plasmid) [110]
Chlamydia trachomatis, sequence accession CP002024 [111]
Phylum Spirochaetes
Spirochaeta thermophila DSM 6192, sequence accession CP001698 [112]
Brachyspira intermedia, sequence accession CP002874 (chromosome) and CP002875 (plasmid) [113]
Phylum Fibrobacteres
Phylum Bacteroidetes
Porphyromonas gingivalis TDC60, sequence accession AP012203 [114]
Krokinobacter sp. strain 4H-3-7-5, sequence accession CP002528 [115]
Lacinutrix sp. strain 5H-3-7-4, sequence accession CP002825 [115]
Bacterium HQM9, sequence accession AFPB00000000 [116]
Anaerophaga sp. Strain HS1, sequence accession AFSL00000000 [117]
Capnocytophaga canimorsus Strain 5, sequence accession CP002113 [118]
Mesoflavibacter zeaxanthinifaciens strain S86, sequence accession AFOE00000000 [119]
Phylum Verrucomicrobia
Phylum Lentisphaerae
Phylum Thermotogae
Kosmotoga olearia Strain TBF 19.5.1, sequence accession CP001634 [120]
Domain Archaea
"Candidatus Nitrosoarchaeum koreensis" MY1, sequence accession AFPU00000000 [121]

Non-Bacterial genomes

North-European Cucumber Cucumis sativus L., sequence accession FI132140-FI136208, GS765762-GS766880, GS815969-GS874855 [122]
Castor bean Ricinus communis organelle genome, sequence accession JF937588(chloroplast), HQ874649 (mitochondria) [123]
Stretch Lagoon Orbivirus Umatilla, sequence accession HQ842619 through HQ842628 [124]
Atlantic cod Gadus morhua, sequence accession CAEA01000001 through CAEA01554869 [125]
Potato Solanum tuberosum L., sequence accession GS025503 through GS026177 [126]
ΦCA82, sequence accession HQ264138 [127]
Paramecium caudatumreveals mitochondria, sequence accession NC001324 [128]
bacteriophage IME08, sequence accession NC_014260 [129]
virus (ILTV), sequence accession HQ_630064 [130]
Australian kangaroo Macropus eugenii, sequence accession ABQO000000000 [131]
Aichi virus, sequence accession FJ890523 [132]
"Candidatus Tremblaya princeps" Strain PCVAL, sequence accession CP002918 [133]

相似文献

12.

Isolation an Aldehyde Dehydrogenase Gene from Metagenomics Based on Semi-nest Touch-Down PCR

Rong Chen Chenglu Li Xiaolin Pei Qiuyan Wang Xiaopu Yin Tian Xie 《Indian journal of microbiology》2014,54(1):74-79

Culture-independent approaches to analyze metagenome are practical choices for rapid exploring useful genes. The mg-MSDH gene, acquired from the hot spring metagenomic, was retrieved full lengths of functional gene using semi-nest touch-down PCR. Two pairs of degenerate primers were used to separate seven conserve partial sequences by semi-nest touch-down PCR. One of them showed similarity with aldehyde dehydrogenase was used as a target fragment for isolating full-length sequence. The full-length mg-MSDH sequence contained a 1,473 bp coding sequence encoding a 490-amino-acid polypeptide and assigned an accession number JQ715422 in Genbank. The upstream sequences TAGGAG of the start codon (GTG), suggested that was a ribosome binding site. The coding sequence of mg-MSDH was ligated to pET-303 vector and the reconstructive plasmid was successfully overexpressed in E. coli. The purified recombinant mg-MSDH enzyme showed propionaldehyde oxidative activity of 3.0 U mg⁻¹ at 37 °C. 相似文献

13.

Genome Sequences of Strains HTCC2148 and HTCC2080, Belonging to the OM60/NOR5 Clade of the Gammaproteobacteria

J. Cameron Thrash Jang-Cheon Cho Steve Ferriera Justin Johnson Kevin L. Vergin Stephen J. Giovannoni 《Journal of bacteriology》2010,192(14):3842-3843

相似文献

14.

Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes

Minakshi Choudhary Jayanand Jasdeep Chatrath Padaria 《Physiology and Molecular Biology of Plants》2015,21(2):187-196

相似文献

15.

Extraordinary Diversity of Immune Response Proteins among Sea Urchins: Nickel-Isolated Sp185/333 Proteins Show Broad Variations in Size and Charge

Lauren S. Sherman Catherine S. Schrankel Kristy J. Brown L. Courtney Smith 《PloS one》2015,10(9)

Effective protection against pathogens requires the host to produce a wide range of immune effector proteins. The Sp185/333 gene family, which is expressed by the California purple sea urchin Strongylocentrotus purpuratus in response to bacterial infection, encodes a highly diverse repertoire of anti-pathogen proteins. A subset of these proteins can be isolated by affinity to metal ions based on multiple histidines, resulting in one to four bands of unique molecular weight on standard Western blots, which vary depending on the individual sea urchin. Two dimensional gel electrophoresis (2DE) of nickel-isolated protein samples followed by Western blot was employed to detect nickel-isolated Sp185/333 (Ni-Sp185/333) proteins and to evaluate protein diversity in animals before and after immune challenge with marine bacteria. Ni-Sp185/333 proteins of the same molecular weight on standard Western blots appear as a broad complex of variants that differ in pI on 2DE Western blots. The Ni-Sp185/333 protein repertoire is variable among animals, and shows a variety of changes among individual sea urchins in response to immune challenges with both the same and different species of bacteria. The extraordinary diversity of the Ni-Sp185/333 proteins may provide significant anti-pathogen capabilities for sea urchins that survive solely on innate immunity. 相似文献

16.

Mcl‐1 inhibition overcomes BET inhibitor resistance induced by low FBW7 expression in breast cancer

Xu Wang Xiaolin Wei Yu Cao Peng Xing 《Journal of cellular and molecular medicine》2022,26(5):1672

While the promise of bromodomains and extraterminal (BET) protein inhibitors (BETis) is emerging in breast cancer (BC) therapy, resistance in these cells to BETis conspicuously curbs their therapeutic potential. FBW7 is an important tumour suppressor. However, the role of FBW7 in BC is not clear. In the current study, our data indicated that the low expression of FBW7 contributes to the drug resistance of BC cells upon JQ1 treatment. shRNA‐mediated FBW7 silencing in FBW7 WT BC cells suppressed JQ1‐induced apoptosis. Mechanistically, it was revealed that this diminished FBW7 level leads to Mcl‐1 stabilization, while Mcl‐1 upregulation abrogates the killing effect of JQ1. Mcl‐1 knockdown or inhibition resensitized the BC cells to JQ1‐induced apoptosis. Moreover, FBW7 knockdown in MCF7 xenografted tumours demonstrated resistance to JQ1 treatment. The combination of JQ1 with a Mcl‐1 inhibitor (S63845) resensitized the FBW7 knockdown tumours to JQ1 treatment in vivo. Our study paves the way for a novel therapeutic potential of BETis with Mcl‐1 inhibitors for BC patients with a low FBW7 expression. 相似文献

17.

Molecular Identification of Cryptosporidium viatorum Infection in a Patient Suffering from Unusual Cryptosporidiosis in West Bengal,India

Sanjib Kumar Sardar Ajanta Ghosal Yumiko Saito-Nakano Shanta Dutta Tomoyoshi Nozaki Sandipan Ganguly 《The Korean journal of parasitology》2021,59(4):409

In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally distributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank. 相似文献

18.

An Isoform of Eukaryotic Initiation Factor 4E from Chrysanthemum morifolium Interacts with Chrysanthemum Virus B Coat Protein

Aiping Song Wanghuai Lou Jiafu Jiang Sumei Chen Zuxia Sun Zhiyong Guan Weimin Fang Nianjun Teng Fadi Chen 《PloS one》2013,8(3)

Background

Eukaryotic translation initiation factor 4E (eIF4E) plays an important role in plant virus infection as well as the regulation of gene translation.

Methodology/Principal Findings

Here, we describe the isolation of a cDNA encoding CmeIF(iso)4E (GenBank accession no. JQ904592), an isoform of eIF4E from chrysanthemum, using RACE PCR. We used the CmeIF(iso)4E cDNA for expression profiling and to analyze the interaction between CmeIF(iso)4E and the Chrysanthemum virus B coat protein (CVBCP). Multiple sequence alignment and phylogenetic tree analysis showed that the sequence similarity of CmeIF(iso)4E with other reported plant eIF(iso)4E sequences varied between 69.12% and 89.18%, indicating that CmeIF(iso)4E belongs to the eIF(iso)4E subfamily of the eIF4E family. CmeIF(iso)4E was present in all chrysanthemum organs, but was particularly abundant in the roots and flowers. Confocal microscopy showed that a transiently transfected CmeIF(iso)4E-GFP fusion protein distributed throughout the whole cell in onion epidermis cells. A yeast two hybrid assay showed CVBCP interacted with CmeIF(iso)4E but not with CmeIF4E. BiFC assay further demonstrated the interaction between CmeIF(iso)4E and CVBCP. Luminescence assay showed that CVBCP increased the RLU of Luc-CVB, suggesting CVBCP might participate in the translation of viral proteins.

Conclusions/Significance

These results inferred that CmeIF(iso)4E as the cap-binding subunit eIF(iso)4F may be involved in Chrysanthemum Virus B infection in chrysanthemum through its interaction with CVBCP in spatial. 相似文献

19.

Draft Genome Sequence of Xylaria grammica EL000614, a Strain Producing Grammicin,a Potent Nematicidal Compound

Sook-Young Park Jongbum Jeon Jung A Kim Mi Jin Jeon Nan Hee Yu Seulbi Kim Ae Ran Park Jin-Cheol Kim Yerim Lee Youngmin Kim Eu Ddeum Choi Min-Hye Jeong Yong-Hwan Lee Soonok Kim 《Mycobiology》2021,49(3):294

An endolichenic fungus, Xylaria grammica strain EL000614, showed strong nematicidal effects against plant pathogenic nematode, Meloidogyne incognita by producing grammicin. We report genome assembly of X. grammica EL000614 comprised of 25 scaffolds with a total length of 54.73 Mb, N50 of 4.60 Mb, and 99.8% of BUSCO completeness. GC contents of this genome were 44.02%. Gene families associated with biosynthesis of secondary metabolites or regulatory proteins were identified out of 13,730 gene models predicted. 相似文献

20.

Prevalence,antimicrobial resistance profile,and characterization of multi-drug resistant bacteria from various infected wounds in North Egypt

Mohamed A. Hassan Sarah Abd El-Aziz Horeya M. Elbadry Samy A. El-Aassar Tamer M. Tamer 《Saudi Journal of Biological Sciences》2022,29(4):2978

Multi-drug resistant (MDR) bacteria associated with wounds are extremely escalating. This study aims to survey different wounds in Alexandria hospitals, North Egypt, to explore the prevalence and characteristics of MDR bacteria for future utilization in antibacterial wound dressing designs. Among various bacterial isolates, we determined 22 MDR bacteria could resist different classes of antibiotics. The collected samples exhibited the prevalence of mono-bacterial infections (60%), while 40% included poly-bacterial species due to previous antibiotic administration. Moreover, Gram-negative bacteria showed dominance with a ratio of 63.6%, while Gram-positive bacteria reported 36.4%. Subsequently, the five most virulent bacteria were identified following the molecular approach by 16S rRNA and physiological properties using the VITEK 2 automated system. They were deposited in GenBank as Staphylococcus haemolyticus MST1 (KY550377), Pseudomonas aeruginosa MST2 (KY550378), Klebsiella pneumoniae MST3 (KY550379), Escherichia coli MST4 (KY550380), and Escherichia coli MST5 (KY550381). In terms of isolation source, S. haemolyticus MST1 was isolated from a traumatic wound, while P. aeruginosa MST2 and E. coli MST4 were procured from hernia surgical wounds, and K. pneumoniae MST3 and E. coli MST5 were obtained from diabetic foot ulcers. Antibiotic sensitivity tests exposed that K. pneumoniae MST3, E. coli MST4, and E. coli MST5 are extended-spectrum β-lactamases (ESBLs) bacteria. Moreover, S. haemolyticus MST1 belongs to the methicillin-resistant coagulase-negative staphylococcus (MRCoNS), whereas P. aeruginosa MST2 exhibited resistance to common empirical bactericidal antibiotics. Overall, the study provides new insights into the prevalent MDR bacteria in Egypt for further use as specific models in formulating antibacterial wound dressings. 相似文献