共查询到20条相似文献,搜索用时 15 毫秒
1.
Microbial treatment of a styrene-contaminated air stream in a biofilter with high elimination capacities 总被引:3,自引:0,他引:3
A styrene-utilizing mixed microbial culture was isolated and utilized in a biofilter for the biological treatment of a contaminated
air stream. Biofilter media consisted of composted wood bark and yard waste. The biofilters were acclimated at 120 s residence
time and further evaluated at 60 and 30 s gas residence times. The biofilters received organic loading rates of up to 350
g/m3 h. The styrene volumetric removal rate was a function of the organic loading rate and increased with increasing loading rates.
Average volumetric removal rates of 69–118 g/m3 h observed in our studies were higher than reported values for styrene biofilters. Average styrene removal efficiencies ranged
from 65% to 75% (maximum 100%). Axial analysis of styrene concentration along the column indicated that the bulk of the styrene
removal occurred in the first section of the biofilter. Analyses of the media indicated that the moisture content of the first
section (50–55% w/w) was significantly lower than in the second and third sections (65–70% w/w). The pressure drops across
the biofilter were low due to the high concentration of large media particles. The total pressure drops were 1–3, 4–6, and
10–16 mm for the 120-, 60-, and 30-s residence time periods, respectively. Journal of Industrial Microbiology & Biotechnology (2001) 26, 196–202.
Received 04 March 2000/ Accepted in revised form 25 January 2001 相似文献
2.
Hee Wook Ryu So Jong Kim Kyung-Suk Cho Tae Ho Lee 《Biotechnology and Bioprocess Engineering》2008,13(3):360-365
In the present study, toluene elimination in the polyurethane (PU) biofilter during long-term (145 day) operation was characterized,
and assessed the effects of changing the inlet loading and space velocity (SV). A very high elimination capacity of 3.7 kg·m−3·h−1 was obtained at an inlet loading of 4.0 kg·m–3·h−1 (inlet toluene concentration of 900 ppmv at a SV of 1,040 h−1). Backwashing with irrigation and compressed air allowed maintenance of a pressure drop of < 80 mm H2O·m−1-filter at an SV of 830 h−1 and an elimination efficiency of > 90% during the 145 day of operation. In conclusion, the PU biofilter can overcome the
problems of clogging caused by excess biomass growth and of low treatment capacities of conventional biofilters. 相似文献
3.
Community structure in a methanotroph biofilter as revealed by phospholipid fatty acid analysis 总被引:1,自引:0,他引:1
The microbial community structure of two biofilters used for the oxidation of methane and organic trace gases generated in landfills was analysed by phospholipid fatty acid composition. Community structure varied with biofilter depth, reflecting varying conditions of substrate supply as well as of organic carbon content, nutrient status and osmotic stress determined by the different materials used for the individual biofilter layers. Both biofilters were dominated by type II methanotrophs. In the biofilter charged with landfill gas containing significant amounts of trace organics, fatty acid 18:1omega7c constituted 87% of the methanotrophic PLFA, while the recognised signature fatty acids 16:1omega8 and 18:1omega8, which were well represented in the other biofilter, were entirely absent. This indicates the development of a highly specific methanotrophic population, presumably as a result of the adaption to continuous organic trace gas exposure. 相似文献
4.
Investigation on the mechanism of H<Subscript>2</Subscript>S removal by biological activated carbon in a horizontal biotrickling filter 总被引:1,自引:0,他引:1
The use of supporting media for the immobilization of microorganisms is widely known to provide a surface for microbial growth and a shelter that protects the microorganisms from inhibitory compounds. In our previous studies, activated carbon (AC) alone used as a support medium for H2S biological removal was proved prompt and efficient in a bench-scale biofilter and biotrickling filter. In this study, the mechanisms of H2S elimination using microbial immobilized activated carbon, i.e., biological activated carbon (BAC), are investigated. A series of BAC as supporting medium were taken from the inlet to outlet of a bench-scale horizontal biotrickling filter to examine the different effects of physical/chemical adsorption and microbial degradation on the overall removal of H2S. The surface properties of BAC together with virgin and exhausted carbon (after H2S breakthrough test, non-microbial immobilization) were characterized using the sorption of nitrogen (Braunner–Emmett–Teller test), scanning electron microscopy (SEM), surface pH, thermal, carbon–hydrogen–nitrogen–sulfur (CHNS) elemental and Fourier transform infrared (FTIR) analyses. Tests of porosity and surface area provide detailed information about the pore structure of BAC along the bed facilitating the understanding of potential pore blockages due to biofilm coating. A correlation between the available surface area and pore volume with the extent of microbial immobilization and H2S uptake is evidenced. SEM photographs show the direct carbon structure and biofilm coated on carbon surface. FTIR spectra, differential thermogravimetric curves and CHNS results indicate less diversity of H2S oxidation products on BAC than those previously observed on exhausted carbon from H2S adsorption only. The predominant oxidation product on BAC is sulfuric acid, and biofilm is believed to enhance the oxidation of H2S on carbon surface. The combination of biodegradation and physical adsorption of using BAC in removal of H2S could lead to a long-term (i.e., years) good performance of biotrickling filters and biofilters based on BAC compared to carbon adsorption only. 相似文献
5.
Styrene vapors from contaminated air were eliminated using long-term adapted mixed microbial culture inoculated on four perlite
packed biofilters (serial arrangement, up-flow configuration). During start-up the inlet concentration of styrene rose from
175 to 1300 mg/m3 of total carbon. The total actual residence time in the four biofilters was 24 s. Styrene was successfully degraded by the
microbial population in the biofilter. An average of 66% of eliminated styrene was transformed to CO2. The removal efficiency of the pollutant was, after 18 d of start-up, nearly 85% at an organic load of 170g/m3 per h. The concentration profiles along the bed height were linear for various pollutant inlet concentrations. The total
amount of microorganisms in analyzed biomass from the biofilters was about 109 per gram of dry packing mass. The moisture content was around 80% in all biofilters. 相似文献
6.
An innovative, coir-pith-based, filter bed for degrading vapor phase toluene in a gas biofilter over 160 days without any
external nutrient supply is reported in this study. Indigenous microflora present in the coir pith as well as in the aerobic
sludge added at the start-up stage metabolized the toluene, and correspondingly, CO2 was produced in the biofilter. Inlet toluene concentration in the range of 0.75 to 2.63 g/m3 was supplied to the biofilter in short acclimation periods. The maximum elimination capacity achieved was 96.75 g/m3·h at 120.72 g/m3·h loading where around 60% was recovered as CO2. The filter bed maintained a stable low-pressure drop (0–4 mm H2O), neutral pH range (6.5–7.5), and moisture content of 60–80% (w/w) throughout the period. In addition to toluene-degrading microbial community, a grazing fauna including rotifer, bacteriovoric
nematode, tardigrade, and fly larvae were also present in the filter bed. The overall performance of the biofilter bed in
pollutant removal and sustainability was analyzed in this study. 相似文献
7.
David van der Ha Sven Hoefman Pascal Boeckx Willy Verstraete Nico Boon 《Applied microbiology and biotechnology》2010,87(6):2355-2363
Effluents of anaerobic digesters are an underestimated source of greenhouse gases, as they are often saturated with methane.
A post-treatment with methane-oxidizing bacterial consortia could mitigate diffuse emissions at such sites. Semi-continuously
fed stirred reactors were used as model systems to characterize the influence of the key parameters on the activity of these
mixed methanotrophic communities. The addition of 140 mg L−1 NH4+–N had no significant influence on the activity nor did a temperature increase from 28°C to 35°C. On the other hand, addition
of 0.64 mg L−1 of copper(II) increased the methane removal rate by a factor of 1.5 to 1.7 since the activity of particulate methane monooxygenase
was enhanced. The influence of different concentrations of NaCl was also tested, as effluents of anaerobic digesters often
contain salt levels up to 10 g NaCl L−1. At a concentration of 11 g NaCl L−1, almost no methane-oxidizing activity was observed in the reactors without copper addition. Yet, reactors with copper addition
exhibited a sustained activity in the presence of NaCl. A colorimetric test based on naphthalene oxidation showed that soluble
methane monooxygenase was inhibited by copper, suggesting that the particulate methane monooxygenase was the active enzyme
and thus more salt resistant. The results obtained demonstrate that the treatment of methane-saturated effluents, even those
with increased ammonium (up to 140 mg L−1 NH4+–N) and salt levels, can be mitigated by implementation of methane-oxidizing microbial consortia. 相似文献
8.
Farshid Ghorbani Shahna Faride Golbabaei Javad Hamedi Hossein Mahjub Hossein Reza Darabi Seyed Jamaleddin Shahtaheri 《Bioprocess and biosystems engineering》2010,33(2):219-226
An adapted bioactive foamed emulsion bioreactor for the treatment of benzene vapor has been developed. In this reactor, bed
clogging was resolved by bioactive foam as a substitute of packing bed for interfacial contact of liquid to gaseous phase.
The pollutant solubility has been increased using biocompatible organic phase in liquid phase and this reactor can be applied
for higher inlet benzene concentration. Experimental results showed a benzene elimination capacity (EC) of 220 g m−3 h−1 with removal efficiency (RE) of 85% for benzene inlet concentration of 1–1.2 g m−3 at 15 s gas residence time in bioreactor. Assessment of benzene concentration in liquid phase showed that a significant amount
of transferred benzene mass has been biodegraded. By optimizing the operational parameters of bioreactor, continuous operation
of bioreactor with high EC and RE was demonstrated. With respect to the results, this reactor has the potential to be applied
instead of biofilter and biotrickling filters. 相似文献
9.
Volatile organic compounds, namely, toluene, trichloroethylene, styrene, etc., disposed off by electronics and polymer industries,
are very harmful. The treatment of VOC laden air through biochemical route is one of the potential options for reduction of
their concentration in parts per million or parts per billion level. Under the present investigation, a 0.05-m diameter and
0.58-m high trickle bed biofilter has been studied for the removal of VOCs namely toluene and trichloroethylene from a simulated
air–VOC mixture using pure strain of Pseudomonas putida (NCIM2650) in immobilized form. Inlet concentrations of VOCs have been varied in two ranges, the lower being 0.20–2.00 g/m3 and higher being 10–20 g/m3, respectively. The Monod type rate kinetics of removal of VOCs has been determined. A three-phase deterministic mathematical
model has been developed taking the simultaneous reaction kinetics and interphase (gas to liquid to biofilm) mass transfer
rate of VOCs into consideration. Experimentally determined kinetic parameters and mass transfer coefficients calculated using
standard correlations have been used. Concentrations have been simulated for all the three phases. Simulated results based
on the model have been compared with the experimental ones for both gas and liquid phases satisfactorily. The mathematical
model validated through the successful comparison with experimental data may be utilized for the prediction of performance
of biofilters undergoing removal of different VOCs in any further investigation and may be utilized for the scale-up of the
system to industrial scale. 相似文献
10.
Treatment of H2S using a horizontal biotrickling filter based on biological activated carbon: reactor setup and performance evaluation 总被引:1,自引:0,他引:1
Biological treatment is an emerging and prevalent technology for treating off-gases from wastewater treatment plants. The most commonly reported odorous compound in off-gases is hydrogen sulfide (H2S), which has a very low odor threshold. A self-designed, bench-scale, cross-flow horizontal biotrickling filter (HBF) operated with bacteria immobilized activated carbon (termed biological activated carbon—BAC), was applied for the treatment of H2S. A mixed culture of sulfide-oxidizing bacteria dominated by Acidithiobacillus thiooxidans acclimated from activated sludge was used as bacterial seed and the biofilm was developed by culturing the bacteria in the presence of carbon pellets in mineral medium. HBF performance was evaluated systematically over 120 days, depending on a series of changing factors including inlet H2S concentration, gas retention time (GRT), pH of recirculation solution, upset and recovery, sulfate accumulation, pressure drop, gas-liquid ratio, and shock loading. The biotrickling filter system can operate at high efficiency from the first day of operation. At a volumetric loading of 900 m3 m–3 h–1 (at 92 ppmv H2S inlet concentration), the BAC exhibited maximum elimination capacity (113 g H2S/m–3 h–1) and a removal efficiency of 96% was observed. If the inlet concentration was kept at around 20 ppmv, high H2S removal (over 98%) was achieved at a GRT of 4 s, a value comparable with those currently reported for biotrickling filters. The bacterial population in the acidic biofilter demonstrated capacity for removal of H2S over a broad pH range (pH 1–7). A preliminary investigation into the different effects of bacterial biodegradation and carbon adsorption on system performance was also conducted. This study shows the HBF to be a feasible and economic alternative to physical and chemical treatments for the removal of H2S. 相似文献
11.
In recent years, biofiltration has been increasingly applied as an air pollution control technology to minimize or eliminate emissions of volatile organic compounds from industrial sources and environmental remediation activities. Although the ability of this technology to maintain high removal efficiency during relatively steady loading conditions has been well established for many waste streams, relatively little research has focused on development of operating strategies that could improve treatment performance during transient loading conditions typical of industrial operations. In the research described herein, two operating strategies were evaluated over a period of 295 days in biofilters treating a model waste gas stream containing a two-component mixture of methyl ethyl ketone (MEK) and toluene. One biofilter was operated as a sequencing batch biofilter (SBB), and the other was operated as a conventional continuous-flow biofilter (CFB). During "normal" steady loading conditions, the model waste stream contained MEK concentrations ranging from 80 to 89 ppmv and toluene concentrations ranging from 28 to 30 ppmv. Both biofilter operating strategies resulted in stable long-term performance with greater than 99% contaminant removal during these normal loading conditions. On a regular basis, the influent MEK and toluene concentrations were temporarily increased to five times the normal influent concentration for the duration of 1 h to test performance during transient "shock loading" conditions. Biofilter performance during the model shock loading conditions demonstrate that SBB operating strategies can result in superior treatment in two important areas: (1) overall mass of contaminants removed and (2) minimum instantaneous removal efficiency. 相似文献
12.
Y. N. Khmelenina B. Ts. Eshinimaev M. G. Kalyuzhnaya Yu. A. Trotsenko 《Microbiology》2000,69(4):460-465
Radioisotopic measurements of the methane consumption by mud samples taken from nine Southern Transbaikal soda lakes (pH 9.5–10.6)
showed an intense oxidation of methane in the muds of Lakes Khuzhirta, Bulamai Nur, Gorbunka, and Suduntuiskii Torom, with
the maximum oxidation rate in the mud of Lake Khuzhirta (33.2 nmol/(ml day)). The incorporation rate of the radioactive label
from14CH4 into14CO2 was higher than into acid-stable metabolites. Optimum pH values for methane oxidation in water samples were 7–8, whereas
mud samples exhibited two peaks of methane oxidation activity (at pH 8.15–9.4 and 5.8–6.0). The majority of samples could
oxidize ammonium to nitrites; the oxidation was inhibited by methane. The PCR amplification analysis of samples revealed the
presence of genes encoding soluble and paniculate methane monooxygenase and methanol dehydrogenase. Three alkaliphilic methanotrophic
bacteria of morphotype I were isolated from mud samples in pure cultures, one of which, B5, was able to oxidize ammonium to
nitrites at pH 7–11. The data obtained suggest that methanotrophs are widely spread in the soda lakes of Southern Transbaikal,
where they can actively oxidize methane and ammonium. 相似文献
13.
Hendy Limbri Cindy Gunawan Torsten Thomas Andrew Smith Jason Scott Bettina Rosche 《PloS one》2014,9(4)
Methane emitted by coal mine ventilation air (MVA) is a significant greenhouse gas. A mitigation strategy is the oxidation of methane to carbon dioxide, which is approximately twenty-one times less effective at global warming than methane on a mass-basis. The low non-combustible methane concentrations at high MVA flow rates call for a catalytic strategy of oxidation. A laboratory-scale coal-packed biofilter was designed and partially removed methane from humidified air at flow rates between 0.2 and 2.4 L min−1 at 30°C with nutrient solution added every three days. Methane oxidation was catalysed by a complex community of naturally-occurring microorganisms, with the most abundant member being identified by 16S rRNA gene sequence as belonging to the methanotrophic genus Methylocystis. Additional inoculation with a laboratory-grown culture of Methylosinus sporium, as investigated in a parallel run, only enhanced methane consumption during the initial 12 weeks. The greatest level of methane removal of 27.2±0.66 g methane m−3 empty bed h−1 was attained for the non-inoculated system, which was equivalent to removing 19.7±2.9% methane from an inlet concentration of 1% v/v at an inlet gas flow rate of 1.6 L min−1 (2.4 min empty bed residence time). These results show that low-cost coal packing holds promising potential as a suitable growth surface and contains methanotrophic microorganisms for the catalytic oxidative removal of methane. 相似文献
14.
Biofiltration of reduced sulphur compounds and community analysis of sulphur-oxidizing bacteria 总被引:2,自引:0,他引:2
Ramírez M Fernández M Granada C Le Borgne S Gómez JM Cantero D 《Bioresource technology》2011,102(5):4047-4053
The present work aims to use a two-stage biotrickling filters for simultaneous treatment of hydrogen sulphide (H2S), methyl mercaptan (MM), dimethyl sulphide (DMS) and dimethyl disulphide (DMDS). The first biofilter was inoculated with Acidithiobacillus thiooxidans (BAT) and the second one with Thiobacillus thioparus (BTT). For separate feeds of reduced sulphur compounds (RSC), the elimination capacity (EC) order was DMDS > DMS > MM. The EC values were 9.8 gMM-S/m3/h (BTT; 78% removal efficiency (RE); empty bed residence time (EBRT) 58 s), 36 gDMDS-S/m3/h (BTT; 94.4% RE; EBRT 76 s) and 57.5 gH2S-S/m3/h (BAT; 92% RE; EBRT 59 s). For the simultaneous removal of RSC in BTT, an increase in the H2S concentration from 23 to 293 ppmv (EBRT of 59 s) inhibited the RE of DMS (97-84% RE), DMDS (86-76% RE) and MM (83-67% RE). In the two-stage biofiltration, the RE did not decrease on increasing the H2S concentration from 75 to 432 ppmv. 相似文献
15.
Biofilters operated for the microbial oxidation of landfill methane at two sites in Northern Germany were analysed for the composition of their methanotrophic community by means of diagnostic microarray targeting the pmoA gene of methanotrophs. The gas emitted from site Francop (FR) contained the typical principal components (CH4, CO2, N2) only, while the gas at the second site Müggenburger Strasse (MU) was additionally charged with non-methane volatile organic compounds (NMVOCs). Methane oxidation activity measured at 22 degrees C varied between 7 and 103 microg CH4 (g dw)(-1) h(-1) at site FR and between 0.9 and 21 microg CH4 (g dw)(-1) h(-1) at site MU, depending on the depth considered. The calculated size of the active methanotrophic population varied between 3 x 10(9) and 5 x 10(11) cells (g dw)(-1) for biofilter FR and 4 x 10(8) to 1 x 10(10) cells (g dw)(-1) for biofilter MU. The methanotrophic community in both biofilters as well as the methanotrophs present in the landfill gas at site FR was strongly dominated by type II organisms, presumably as a result of high methane loads, low copper concentration and low nitrogen availability. Within each biofilter, community composition differed markedly with depth, reflecting either the different conditions of diffusive oxygen supply or the properties of the two layers of materials used in the filters or both. The two biofilter communities differed significantly. Type I methanotrophs were detected in biofilter FR but not in biofilter MU. The type II community in biofilter FR was dominated by Methylocystis species, whereas the biofilter at site MU hosted a high abundance of Methylosinus species while showing less overall methanotroph diversity. It is speculated that the differing composition of the type II population at site MU is driven by the presence of NMVOCs in the landfill gas fed to the biofilter, selecting for organisms capable of co-oxidative degradation of these compounds. 相似文献
16.
Greenhouse gas fluxes from the eutrophic Temmesjoki River and its Estuary in the Liminganlahti Bay (the Baltic Sea) 总被引:2,自引:0,他引:2
Hanna Silvennoinen Anu Liikanen Jaana Rintala Pertti J. Martikainen 《Biogeochemistry》2008,90(2):193-208
We studied concentrations of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) in the eutrophic Temmesjoki River and Estuary in the Liminganlahti Bay in 2003–2004 and evaluated the atmospheric fluxes
of the gases based on measured concentrations, wind speeds and water current velocities. The Temmesjoki River was a source
of CO2, CH4 and N2O to the atmosphere, whereas the Liminganlahti Bay was a minor source of CH4 and a minor source or a sink of CO2 and N2O. The results show that the fluxes of greenhouse gases in river ecosystems are highly related to the land use in its catchment
areas. The most upstream river site, surrounded by forests and drained peatlands, released significant amounts of CO2 and CH4, with average fluxes of 5,400 mg CO2–C m−2 d−1 and 66 mg CH4–C m−2 d−1, and concentrations of 210 μM and 345 nM, respectively, but N2O concentrations, at an average of 17 nM, were close to the atmospheric equilibrium concentration. The downstream river sites
surrounded by agricultural soils released significant amounts of N2O (with an average emission of 650 μg N2O–N m−2 d−1 and concentration of 22 nM), whereas the CO2 and CH4 concentrations were low compared to the upstream site (55 μM and 350 nM). In boreal regions, rivers are partly ice-covered
in wintertime (approximately 5 months). A large part of the gases, i.e. 58% of CO2, 55% of CH4 and 36% of N2O emissions, were found to be released during wintertime from unfrozen parts of the river. 相似文献
17.
Methane is one of the strongest of the greenhouse gases, being 30-fold more radiatively active than carbon dioxide on a molar
basis. In addition, its atmospheric concentrations have increased by 1% per year since the Industrial Revolution. As such,
the dynamics of methane is of great importance for the prediction of global climatic changes caused by increasing concentrations
of greenhouse gases in the atmosphere. One of the most important biological sinks for methane is forest soils, where methanotrophic
bacteria oxidize methane to carbon dioxide. Based on data mined from a review of the literature, we determined that the mean
methane oxidation rate was 1.90 mg CH4 m−2 day−1 and that the main variables controlling this rate were soil water content and inorganic nitrogen in the soils. In contrast,
the effects of temperature and pH are minimal. In addition to reviewing the literature, we monitored methane oxidation rates
in a temperate forest soil in Korea on a monthly basis for a year, using a static chamber method. The mean oxidation rate
was 1.96 mg CH4 m−2 day−1 and was positively correlated with nitrate concentration in the soil. 相似文献
18.
Hydrogen sulfide degradation characteristics of Bordetella sp. Sulf-8 in a biotrickling filter 总被引:1,自引:0,他引:1
Grace M. Nisola Enkhdul Tuuguu Danvir Mark D. Farnazo Mideok Han Younghee Kim Eulsaeng Cho Wook-Jin Chung 《Bioprocess and biosystems engineering》2010,33(9):1131-1138
The applicability of Bordetella sp. Sulf-8 to degrade Hydrogen Sulfide (H2S) gas in a biotrickling system was investigated. The isolate is a heterotrophic gram-negative, catalase- and oxidase-positive,
rod-shaped bacterium which can metabolize thiosulfate or sulfide into sulfate. The mesophilic Bordetella sp. Sulf-8 can grow within a wide pH range using yeast as carbon source, with or without the presence of sulfur. In batch
experiments, kinetic constants such as maximum specific growth rate (μ
max = 0.12 1/h), saturation constant (K
S = 0.017 g/L), and specific sulfur removal rate (88 mg S/g cells h) were obtained. In biotrickling experiments removal efficiencies
were satisfactory, but the system performance was observed to be more influenced by empty bed residence time than by H2S feed gas concentration. Critical and maximum elimination capacities were 78.0 and 94.5 g H2S/m3 day, respectively. Macrokinetic analysis of the biotrickling system revealed maximum H2S removal rate V
max = 15.97 g S/kg media-day and half saturation constant K
S′ = 12.45 ppmv. 相似文献
19.
Gabriel Vigueras Sonia Arriaga Keiko Shirai Marcia Morales Sergio Revah 《Biotechnology letters》2009,31(8):1203-1209
Rhinocladiella similis biodegraded volatile organic compounds (VOCs) of different polarity in gas-phase biofilters. Elimination capacities, (EC)
of 74 ghexane m−3 h−1, 230 gethanol m−3 h−1, 85 gtoluene m−3 h−1 and 30 gphenol m−3 h−1 were obtained. EC values correlated with the solubility of the VOCs. R. similis grown with n-hexane or ethanol in biofilters packed with Perlite showed that the surface hydrophobicity was higher with n-hexane
than ethanol. The hydrophobin-like proteins extracted from the mycelium produced with n-hexane (15 kDa) were different from
those in the ethanol biofilter (8.5 kDa and 7 kDa). 相似文献
20.
Tae Gwan Kim Eun-Hee Lee Kyung-Suk Cho 《Applied microbiology and biotechnology》2013,97(14):6549-6559
Effects of nonmethane volatile organic compounds (NMVOCs) on methanotrophic biofilter were investigated. Laboratory-scale biofilters packed with pumice and granular-activated carbon (10:1, w/w) were operated with CH4 and NMVOCs including dimethyl sulfide (DMS) and benzene/toluene (B/T). DMS alone exhibited a positive effect on the methanotrophic performance; however, the coexistence of B/T removed this effect. B/T alone exerted no effect on the performance. Pyrosequencing and quantitative PCR revealed that the NMVOCs strongly influenced the bacterial and methanotrophic communities but not the population density of methanotrophs. DMS alone diversified and changed both bacterial and methantrophic communities, but its effects were nullified by the presence of B/T. Canonical correspondence analysis revealed significant correlations between the NMVOCs and community composition and significant interaction between DMS and B/T. DMS did not affect the distribution of types I/II methanotrophs (60/40), while B/T increased the abundance of type I to 82 %. DMS and B/T favored the growth of the methanotrophic bacteria Methylosarcina and Methylomonas, respectively. These results suggest that NMVOCs can be a significant abiotic factor influencing methane biofiltration. 相似文献