共查询到20条相似文献,搜索用时 15 毫秒
1.
In this article, we present new laboratory results examining the relative importance of HCO− 3 transport and extracellular carbonic anhydrase (eCA) in 17 marine diatom species. We observed significant variability in both HCO− 3 transport and eCA expression across a range of diatom species with different cell morphologies. All species we examined took up HCO− 3 through a direct transport mechanism, with the fraction of HCO− 3 transport ranging from 40 to 95% of total C uptake. eCA expression also varied significantly, with catalytic enhancement factors ranging approximately 10-fold among species. There was a significant positive correlation between HCO− 3 transport and eCA expression among the test species. However, neither HCO− 3 transport nor eCA expression was significantly correlated to cell growth rates or surface area to volume ratios. We did observe weak positive trends between the ratio of C demand:supply and HCO− 3 utilization/eCA expression, but these were not statistically significant. We are thus unable to provide a mechanistic explanation for the apparent variability in HCO− 3 transport and eCA expression in marine diatoms. This variability may, nonetheless, have important implications for the physiological ecology of oceanic diatoms. 相似文献
2.
为了认识海洋浮游藻类在碳充足和碳受限条件下对水体中溶解无机碳(DIC)的利用方式与可能机理,对13种海洋浮游藻类在不同pH和CO2浓度及不同DIC条件下细胞外碳酸酐酶(CA)的活性进行了分析测定.结果显示:13种藻中,只有Amphidinium carterae和Prorocentrum minimum在碳充足条件下具细胞外CA活性.Melosira sp.、Phaeodactylum tricornutum、Skeletonema costatum、Thalassiosira rotula、Emiliania huxleyi和Pleurochrysis carterae则在碳受限条件下才具细胞外CA活性.Chaetoceros compressus、Glenodinium foliaceum、Coccolithus pelagicus、 Gephrocapsa oceanica和Heterosigma akashiwo即使在碳受限条件下也未检测到细胞外CA活性.应用封闭系统中pH漂移技术和阴离子交换抑制剂4′4′-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS)等的研究表明,Coc. pelagicus和G. oceanica可通过阴离子交换机制进行HCO-3的直接利用.H. akashiwo没有潜在的HCO-3直接利用或细胞外CA催化的HCO-3利用. 相似文献
3.
Inhibition of electron transport through photosystem II (PS II) by formate (HCO− 2 ) or nitrite (NO− 2 ) in the presence or absence of chloride ions was studied. The inhibition induced by HCO− 2 or NO− 2 is overcome by HCO− 3 more in the presence, than in the absence of Cl− . The data on electron transport are supported by chlorophyll a fluorescence measurements. In experiments. In experiments in which water oxidation was blocked. Cl− was found to facilitate electron transport between bound quinone A (Q− A ) and the plastoquinone (PQ) pool. It can thus be concluded that in addition to the well known site of action of Cl− on water oxidation, another site of Cl− action is between Q− A and the PQ pool. 相似文献
4.
Calcification in aquatic plants 总被引:1,自引:0,他引:1
MICHAEL A. BOROWITZKA 《Plant, cell & environment》1984,7(6):457-466
Abstract. The CaCO3 deposits of aquatic plants may be intra-, inter- and extracellular. Calcification is mainly the result of photosynthetic CO2 or HCO− 3 assimilation. This raises the local pH and CO2− 3 concentration resulting from shifts in the dissolved inorganic carbon equilibrium, due to either net CO2 depletion as in Halimeda or localized OH− efflux (or H+ influx) as in Chara. The plant cell wall may be important in CaCO3 nucleation by acting as an epitaxial substratum or template, or by creating a microenvironment enriched in Ca2+ compared to Mg2+ . Hypotheses on the reason for the lack of calcification in many aquatic plants are presented. 相似文献
5.
Nitrite in the root zone and its effects on ion uptake and growth of wheat seedlings 总被引:1,自引:0,他引:1
The immediate and posteffects of various concentrations of NaNO2 on ion uptake of wheat ( Triticum aestivum L. cv. GK Öthalom) seedlings were studied at different pH values. Without pretreatment, the higher the concentration of NaNO2 the greater was the decrease in uptake of K+ into the roots, both at pH 4 and pH 6. At pH 6 but not at pH 4 the reverse was true when the seedlings were pretreated with NaNO2 . Due to the high Na+ content of the roots, an effect of Na+ in this process cannot be excluded. Nitrite was taken up by the roots more rapidly than nitrate. Nitrite at 0.1 m M in the medium induced the development of an uptake system for both NO− 2 and NO− 3 in wheat roots. At higher concentrations pretreatment with NO− 2 decreased NO− 3 uptake by the roots, but NO3 did not inhibit the uptake of NO2 . The toxic effect of NO− 2 was strongly pH dependent. Lower pH of the external solution led to an increased inhibition by NO− 2 of both ion uptake and growth of seedlings. The inhibitory effect of NO− 2 differed considerably for roots and shoots. The roots and especially the root hairs were particularly sensitive to NO− 2 treatment. 相似文献
6.
C.E. DEANE-DRUMMOND 《Plant, cell & environment》1985,8(2):105-110
Abstract. Net NO3 uptake by NO− 3 deficient Chara cells was used to calculate [NO− 3 ]c assuming that the cytoplasm occupies 10% total volume and that nitrate reduction and storage are negligible (i.e. maximum [NO− 3 ]c was calculated). A linear relationship was found between NO− 3 efflux and [NO− 3 ]c . There was an initial burst of NO− 3 efflux when NH+ 4 was added, followed by a slower efflux rate which matched influx rate such that net NO− 3 uptake was zero. Over 50% of NO− 3 that had been taken up in 2 h was lost within the first 5 min of NH+ 4 addition. The Nernst equation was used to predict the direction of the electrochemical driving force for NO− 3 entry. Under the experimental conditions used NO− 3 efflux is actively transported. The differential involvement of both NO− 3 influx and NO− 3 efflux in the regulation of NO− 3 uptake is discussed and a model is proposed to account for these results which envisages discrete NO− 3 influx and NO− 3 efflux carriers. 相似文献
7.
Haemoglobin, methaemoglobin, blood nitrite concentration and acid-base balance were measured in European eel Anguilla anguilla following exposure to 0 (control), 0·142, 0·356, 0·751 and l·549 mM nitrite in fresh water for 24 h. Blood GOT (glutamate oxaloacetate transaminase) and GPT (glutamate pyruvate transaminase) activities and whole animal ammonia-N and urea-N excretions were also measured. Blood nitrite, methaemoglobin, PO 2 (oxygen partial pressure), GOT, and whole animal ammonia-N excretion and urea-N excretion increased directly with increasing ambient nitrite concentrations, whereas blood pH, PCO 2 , and HCO− 3 were inversely related to ambient nitrite concentration. An accumulation of nitrite in the blood of A. anguilla following 24 h exposure to elevated ambient nitrite as low as 0·751 mM increased its blood methaemoglobin, PO 2 , GOT and nitrogen excretion, but decreased its PCO 2 (carbon dioxide partial pressure), HCO− 3 and functional haemoglobin. 相似文献
8.
CYRUS J. BACCHI E. I. CIACCIO DAVID B. KABACK S. H. HUTNER 《The Journal of eukaryotic microbiology》1970,17(2):305-311
SYNOPSIS. Fractions containing soluble enzymes from Crithidia fasciculata had an ADP-linked phosphoenolpyruvate (PEP) carboxykinase. The enzyme produced ATP and oxaloacetate (OAA) from PEP, ADP and HCO− 3 . OAA was determined as the endproduct of reactions by forming the 2,4-dinitrophenylhydrazone derivative; the hydrazone was identified by thin-layer chromatography. Approximate Michaelis constants (PEP, Mg, HCO− 3 , ADP) were determined spectrophotometrically by linking OAA production to malic dehydrogenase. The PEP carboxykinase did not utilize GDP, UDP or IDP as cofactors; the metal requirement was also satisfied by Mn. The enzyme was inhibited by the biotin antagonists avidin and desthiobiotin.
A pyruvate carboxylase was also present in the preparations, generating OAA from pyruvate and ATP. The role of both enzymes in OAA production and subsequent production of succinate is discussed with regard to C. fasciculata and other trypanosomatids. 相似文献
A pyruvate carboxylase was also present in the preparations, generating OAA from pyruvate and ATP. The role of both enzymes in OAA production and subsequent production of succinate is discussed with regard to C. fasciculata and other trypanosomatids. 相似文献
9.
The uptake of the auxin type herbicide 2,4-D into rice seedlings ( Oryza sativa L. cv. Dunghan Shali) and its effects on the K+ , NH+ 4 and NO3 ion uptake and the K+ content were investigated at different pH values. A short incubation of the roots in 0.01 m M 2,4-D caused a marked ion uptake inhibition only at low pH. The non-auxin type herbicide benthiocarb did not produce such an inhibitory effect. Lowering of the pH in the external medium led to an increased 2,4-D uptake by the roots. These results can be explained by the increased H+ permeability of the membranes, allowing a more rapid entrance of 2,4-D into the root cells, thereby inhibiting the active ion uptake. Rice roots not subjected to 2,4-D treatment responded to H+ stress with an increased anomalous K+ uptake and a decreased K+ content. With reference to the effects of pH changes on the ion and 2,4-D uptake, possible transport mechanism of NH+ 4 and 2,4-D are briefly discussed. 相似文献
10.
The mechanism of bicarbonate assimilation by the polar leaves of Potamogeton and Elodea. CO2 concentrations at the leaf surface 总被引:2,自引:2,他引:0
H. B. A. PRINS J. F. H. SNEL P. E. ZANSTRA R. J. HELDER 《Plant, cell & environment》1982,5(3):207-214
Abstract. Photosynthetic utilization of HCO, in leaves of Poiamogeton and Elodea occurs at the lower leaf side, with subsequent OH∼ release at the upper side. It is accompanied by transport of cations, in the present experiment K +, across the leaf. The resulting pH and K+ concentration changes near the leaf surface were recorded with miniature electrodes. From the pH and K+ concentration the concentrations of the different inorganic carbon species were calculated and compared with photosynthetic O, production. HCO− 3 utilization is accompanied by a drastic increase in the free CO2 concentration near the lower epidermis. Experiments with CO2 − and HCO3 − free solutions showed an oscillating acidification near the lower epidermis and alkalinization near the upper epidermis. It is concluded that the acidification results from the activity of light-dependent H+ pumps. The finding that an increase in pH at the upper side always coincided with a decrease at the lower in these experiments shows that the H+ pumps and the OH− extruding mechanism are coupled although occurring in different cell layers. Previously we have suggested that the first step in the process of photosynthetic HCO3 − utilization is external conversion of HCO3 − " by acidification caused by light-dependent H+ pumps. The present results strongly support this hypothesis. Two possible pathways for the accompanying K + transport are discussed. The model presented here explains the known inhibiting effects of buffers and high pH on photosynlhetic HCO3 − utilization. 相似文献
11.
Effect of root perturbation and excision on nitrate influx and efflux in barley (Hordeum vulgare) seedlings 总被引:2,自引:0,他引:2
The effects of perturbation and excision on net NO- 3 , uptake, influx and efflux in roots of 8-day-old barley ( Hordeum vulgare L.) seedlings induced with NO- 3 or NO- 2 were determined. Perturbation was simulated by mechanically striking the intact roots with a glass rod. Perturbation or excision of roots and subsequent division into small segments had little effect on NO- 3 influx, but briefly inhibited net uptake which recovered within a few min. While in perturbed roots net uptake rates recovered to the same level as in control roots, full recovery did not occur in excised roots. Inhibition of net uptake was due to stimulation of NO- 3 efflux. The recovery time and level of inhibition of net NO- 3 uptake and/or stimulation of efflux were a function of extent of perturbation, or the number of segments following excision, and root NO- 3 concentration. NO- 3 efflux was further stimulated when roots were perturbed after cytoplasmic NO- 3 had been depleted, indicating that both the plasmalemma and tonoplast may be affected. In excised roots both NO- 3 influx and efflux decreased with age due to depletion of energy sources. The results indicate that root perturbation and excision had no effect on NO- 3 influx but inhibited net uptake by stimulating efflux. 相似文献
12.
S. Abarzua R. Altenburger R. Callies L.-H. Grimme A. Mayer D. Leibfritz U. Schiewer 《Physiologia plantarum》1993,89(3):659-663
Over a period of several days, rhythmic changes in extracellular NH+ 4 concentration take place in cultures of the cyanobacterium Microcystis firma (Bré et Lenorm.) Schmidle, strain Gromov/St. Petersb. 398, under conditions of restricted CO2 supply and light/dark alternation. The changes are enhanced by nitrate supply. Among the various processes generating intracellular NH+ 4 (NH4 4 uptake, NO− 3 reduction, protein and amino acid degradation, photorespiration), NO− 3 reduction appears as the one most important. This can be concluded from experiments with and without nitrate and/or ammonium in the medium. In the presence of saturating CO2 , continuous light, or continuous darkness, rhythmic NH+4 4 oscillations are not induced. Studies of the incorporation of NH+ 4 nitrogen by in vivo 15 N-NMR show that if CO2 is supplied, 15 N is accumulated in several components with the following time course: in the first hour in Gln (δ), in the second hour in the α-amino groups of most nonbranched amino acids, in the third hour in γ-aminobutyric acid (GABA), Orn (δ) and Lys (ε), and in the sixth hour in Ala. Carbon limitation, however, results in accumulation of label in the amide nitrogen of glutamine only. 相似文献
13.
Uptake regions of inorganic nitrogen in roots of carob seedlings 总被引:2,自引:0,他引:2
Three-week-old seedlings of carob ( Ceratonia siliqua L. cv. Mulata) were grown for 9 weeks under different root temperatures (20, 30 and 40°C) at pH values of 5, 7 and 9 with nitrate or ammonium as nitrogen source. Nitrogen uptake rates were determined by depletion from the medium and decreased with distance from the apex. The decline of nitrogen uptake rates along the roots depended on the form of inorganic nitrogen in the medium as well as on pH and temperature, such that the NO− 3 and NH+ 4 ions were taken up essentially by the root tips (0–2 cm) through processes requiring energy. The uncharged NH3 species entered passively, through the mature parts of the root (2–10 cm). Root zone temperature and pH affect the NH+ 4 /NH3 equilibrium in the nutrient solution and, consequently, the uptake areas of the root for these ions. Furthermore. while root tip uptake of nitrogen is energy dependent, uptake through mature root areas is essentially passive and seems to depend on a well developed apparent free space. 相似文献
14.
Spore germination of Bacillus stearothermophilus was progressively inhibited as the concentrations of sodium bicarbonate (NaHCO3 ) in the germination media increased from 0% to 1·0% (w/v). The inhibitory effect of NaHCO3 was attributed to the release of HCO− 3 and its alkaline properties, each of which played a different role. At low concentrations (< 0·3%), the inhibitory effect of NaHCO3 was mainly due to bicarbonate. As NaHCO3 increased from 0·3% to higher concentrations, the effect of HCO− 3 reached a plateau while the alkalinating effect became the more dominant inhibitory factor. Fourier transform infrared (FTIR) analysis reveals that sodium bicarbonate reacted with the carboxyl group (1570 cm−1 ) of some acidic amino-acid residues of protein in the spore, leading to a less orientated structure. A shift of two units towards the longer frequency for carboxyl groups indicates that a stronger interaction was formed between the carboxyl group and the Na+ ion. The largest ratio of peak height between the absorbance of carboxylate (1570 cm−1 ) and of amide II (1546 cm−1 ) of spores after pretreatment with 0·3% sodium bicarbonate reflects the biggest structural alterations of keratin-like proteins in the spore. The role of NaHCO3 in enhancing the sporicidal effect of glutaraldehyde is discussed. 相似文献
15.
The NO− 3 -triggered induction of nitrate reductase (NR; EC 1.6.6.2) in the bryophyte Sphagnum magellanicum Brid. has been studied, using in vivo and in vitro assays as well as immunological methods. The time-course of induction was triphasic with maximal NR activity after 6–8 h. Results obtained from Western blots show that NR is synthesized de novo after NO− 3 application. The inhibitory effect of cycloheximide on NR induction corroborated this conclusion. Light enhanced the NO− 3 -triggered NR induction. The enzyme activity, measured in vivo, increased more than the in vitro activity. No evidence for phytochrome control of NR was found. Nitrate uptake, in contrast to NR activity, showed no lag period after NO− 3 application and, under the experimental conditions used, was not rate limiting for NR induction. Neither light nor a NO− 3 pretreatment significantly affected NO− 3 uptake. 相似文献
16.
Carbonic anhydrases in higher plants and aquatic microorganisms 总被引:3,自引:1,他引:2
At physiological pH-values CO2 and HCO− 3 are the dominant inorganic carbon species and the interconversion between both is catalyzed by carbonic anhydrase (EC 4.2.1.1). This enzyme is widely distributed among photosynthetic organisms. In the first part of the review, the similarities and the differences of carbonic anhydrases from plants and animals are briefly described. In the second part recent advances in molecular biology to understand the structure of carbonic anhydrase from higher terrestrial plants as well as its involvement in photosynthetic CO2 fixation are summarized. Lastly, the review deals with the presence of carbonic anhydrase in aquatic organisms including cyanobacteria, microalgae, macroalgae and angiosperms. Evidence for the presence of extracellular and intracellular isozymes in these organisms are discussed. The properties and function(s) of carbonic anhydrase during the operation of the inorganic carbon concentrating mechanism are also described. 相似文献
17.
Abstract The utilization of NO− 3 , NO− 2 and NH+ 4 was studied in whole filaments and isolated heterocysts of Anabaena 7120 (ATCC27893). NO− 3 - and NO− 2 -uptake were detectable in whole filaments but not in heterocysts, whereas NH+ 4 -uptake was detectable in both. Activity of NO− 3 -reductase was present in cell-free extracts of whole filaments but not of heterocysts, whereas activities of NO− 2 -reductase and glutamine synthetase were present in both. NO− 3 -uptake and reductase activities could not be induced in heterocysts even after prolonged incubation in NO− 3 medium. It is suggested that NO− 3 -metabolism in heterocysts is impaired due to a selective and irreversible loss of NO− 3 -uptake and reductase systems resulting in the abolition of competition for molybdenum cofactor (Mo-Co) and reductant between nitrogenase and NO− 3 -reductase, and an increase in glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase levels. 相似文献
18.
A range of marine photosynthetic picoeukaryote phytoplankton species grown in culture were screened for the presence of extracellular carbonic anhydrase (CAext ), a key enzyme in inorganic carbon acquisition under carbon- limiting conditions in some larger marine phytoplankton species. Of the species tested, extracellular carbonic anhydrase was detected only in Micromonas pusilla Butcher. The rapid, light-dependent development of CAext when cells were transferred from carbon-replete to carbon-limiting conditions was regulated by the available free- CO2 concentration and not by total dissolved inorganic carbon. Kinetic studies provided support for a CO2 - concentrating mechanism in that the K 0.5 [CO2 ] (i.e. the CO2 concentration required for the half-maximal rate of photosynthesis) was substantially lower than the K m [CO2 ] of Rubisco from related taxa, whilst the intracellular carbon pool was at least seven fold greater than the extracellular DIC concentration, for extracellular DIC values 1.0 m m .
It is proposed that when the flux of CO2 into the cell is insufficient to support the photosynthetic rate at an optimum photon irradiance, the development of CAext increases the availability of CO2 at the plasma membrane. This ensures rapid acclimation to environmental change and provides an explanation for the central role of M. pusilla as a carbon sink in oligotrophic environments. 相似文献
It is proposed that when the flux of CO
19.
The acquisition and accumulation of inorganic carbon by the unicellular green alga Chlorella ellipsoidea 总被引:3,自引:0,他引:3
Abstract. The uptake and accumulation of inorganic carbon has been investigated in Chlorella ellipsoidea cells grown at acid or alkaline pH. Carbonic anhydrase (CA) was detected in ceil extracts but not in intact cells and CA activity in acid-grown cells was considerably less than that in alkali-grown cells. Both cell types demonstrates low K1/2 (CO2 ) values in the range pH 7.0–8.0 and these were unaffected by O2 concentration. The CO2 compensation concentrations of acid- and alkali-grown cells suspended in aqueous media were not significantly different in the range of pH 6.0–8.0, but at pH 5.0, the CO2 compensation concentrations of acid-grown cells (57.4cm3 m−3 ) were lower than those of alkali-grown cells (79.2cm3 m−3 ). The rate of photo-synthetic O2 evolution in the range pH 7.5–8.0 exceeded the calculated rate of CO2 supply two- to three-fold, in both acid- and alkali-grown cells, indicating that HCO3 − was taken up by the cells. Accumulation of inorganic carbon was measured at pH 7.5 by silicone-oil centri-fugation, and the concentration of unfixed inorganic carbon was found to be 5.1 mol m−3 in acid-grown and 6.4mol m−3 in alkali-grown cells. These concentrations were 4.6- and 5.9-fold greater than in the external medium. These results indicate that photorespiration is suppressed in both acid- and alkali-grown cells by an intracellular accumulation of inorganic carbon due, in part, to an active uptake of bicarbonate. 相似文献
20.
Assaf Sukenik Dan Tchernov Aaron Kaplan Emma Huertas Luis M. Lubian Alexander Livne 《Journal of phycology》1997,33(6):969-974
Uptake, efflux and utilization of inorganic carbon were investigated in the marine eustigmatophyte Nannochloropsis sp. grown under an air level of CO2. Maximal photosynthetic rate was hardly affected by raising the pH porn 5.0 to 9.0. The apparent photosynthetic affinity for dissolved inorganic carbon (DIC) was 35 μM DIC between pH 6.5 to 9.0, but increased approximately threefold at pH 5.0 suggesting that HCO3- was the main DIC species used from the medium. No external carbonic anhydrase (CA) activity could be detected by the pH drift method. However, application of ethoxyzolamide (an inhibitor of CA) resulted an a significant inhibition of photosynthetic O2 evolution and carbon utilization, suggesting involvement of internal CA or CA-like activity in DIC utilization. Under high light conditions, the rate of HCO3? uptake and its internal conversion to CO2 apparently exceeded the rate of carbon fixation, resulting in a large leak of CO2 from the cells to the external medium. When the cells were exposed to low DIC concentrations, the ratio of internal to external DIC concentration was about eight. On the other hand, in the presence of 2 mM DIC, conditions prevailing in the marine environment, the internal concentration of DIC was only 50% higher than the external one. 相似文献