共查询到20条相似文献,搜索用时 31 毫秒
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Narusaka Y Nakashima K Shinwari ZK Sakuma Y Furihata T Abe H Narusaka M Shinozaki K Yamaguchi-Shinozaki K 《The Plant journal : for cell and molecular biology》2003,34(2):137-148
Many abiotic stress-inducible genes contain two cis-acting elements, namely a dehydration-responsive element (DRE; TACCGACAT) and an ABA-responsive element (ABRE; ACGTGG/TC), in their promoter regions. We precisely analyzed the 120 bp promoter region (-174 to -55) of the Arabidopsis rd29A gene whose expression is induced by dehydration, high-salinity, low-temperature, and abscisic acid (ABA) treatments and whose 120 bp promoter region contains the DRE, DRE/CRT-core motif (A/GCCGAC), and ABRE sequences. Deletion and base substitution analyses of this region showed that the DRE-core motif functions as DRE and that the DRE/DRE-core motif could be a coupling element of ABRE. Gel mobility shift assays revealed that DRE-binding proteins (DREB1s/CBFs and DREB2s) bind to both DRE and the DRE-core motif and that ABRE-binding proteins (AREBs/ABFs) bind to ABRE in the 120 bp promoter region. In addition, transactivation experiments using Arabidopsis leaf protoplasts showed that DREBs and AREBs cumulatively transactivate the expression of a GUS reporter gene fused to the 120 bp promoter region of rd29A. These results indicate that DRE and ABRE are interdependent in the ABA-responsive expression of the rd29A gene in response to ABA in Arabidopsis. 相似文献
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Two cysteine proteinase inhibitors from Arabidopsis thaliana, AtCYSa and AtCYSb, increasing the salt, drought, oxidation and cold tolerance 总被引:1,自引:0,他引:1
Two cysteine proteinase inhibitors (cystatins) from Arabidopsis thaliana, designated AtCYSa and AtCYSb, were characterized. Recombinant GST-AtCYSa and GST-AtCYSb were expressed in Escherichia coli and purified. They inhibit the catalytic activity of papain, which is generally taken as evidence for cysteine proteinase inhibitor function. Northern blot analyses showed that the expressions of AtCYSa and AtCYSb gene in Arabidopsis cells and seedlings were strongly induced by multiple abiotic stresses from high salt, drought, oxidant, and cold. Interestingly, the promoter region of AtCYSa gene contains a dehydration-responsive element (DRE) and an abscisic acid (ABA)-responsive element (ABRE), which identifies it as a DREB1A and AREB target gene. Under normal conditions, AtCYSa was expressed in 35S: DREB1A and 35S: AREB1 plants at a higher level than in WT plants, while AtCYSa gene was expressed in 35S: DREB2A plants at the same level as in WT plants. Under stress conditions (salt, drought and cold), AtCYSa was expressed more in all three transgenic plants than in WT plants. Over-expression of AtCYSa and AtCYSb in transgenic yeast and Arabidopsis plants increased the resistance to high salt, drought, oxidative, and cold stresses. Taken together, these data raise the possibility of using AtCYSa and AtCYSb to genetically improve environmental stresses tolerance in plants. 相似文献
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Ahamed Khan Ankita Shrestha Kashyap Bhuyan Indu B. Maiti Nrisingha Dey 《Plant molecular biology》2018,96(1-2):179-196
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A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis 总被引:5,自引:0,他引:5
Schramm F Larkindale J Kiehlmann E Ganguli A Englich G Vierling E von Koskull-Döring P 《The Plant journal : for cell and molecular biology》2008,53(2):264-274
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Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems 总被引:20,自引:0,他引:20
Maruyama K Sakuma Y Kasuga M Ito Y Seki M Goda H Shimada Y Yoshida S Shinozaki K Yamaguchi-Shinozaki K 《The Plant journal : for cell and molecular biology》2004,38(6):982-993
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AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis 总被引:18,自引:0,他引:18
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Fujita Y Fujita M Satoh R Maruyama K Parvez MM Seki M Hiratsu K Ohme-Takagi M Shinozaki K Yamaguchi-Shinozaki K 《The Plant cell》2005,17(12):3470-3488