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1.
A comparative study of the number and distribution of chromocentres in interphase nuclei and mean chiasma frequency at diakinesis has been made in three varietal populations of radish (Raphanus sativus L.), Scarlet Globe, Japanese White and Chinese White. The study showed a significant difference between the varietal populations in mean chiasma frequency and number of chromocentres (P<0.001), indicating that these nuclear characters are genotypically controlled. The correlation analysis revealed a significant negative correlation between chromocentres and chiasma frequency (r= -0.87). It was concluded that an increase in the amount of constitutive heterochromatin, as inferred by chromocentre counts, adversely affects the chiasma frequency and, consequently, genetic recombination in radish. 相似文献
2.
Plethodontid salamanders in the genus Oedipina are characterized by a strongly heteromorphic sex-determining pair of X/Y chromosomes. The telocentric X chromosome and the subtelocentric Y chromosome are clearly distinguished from the autosomes and their behavior during meiosis can be sequentially followed in squash preparations of spermatocytes. In Oedipina the sex chromosomes are not obscured by an opaque sex vesicle during early meiotic stages, making it possible to observe details of sex bivalent structure and behavior not directly visible in other vertebrate groups. The sex chromosomes can first be distinguished from autosomal bivalents at the conclusion of zygotene, with X and Y synapsed only along a short segment at their non-centromeric ends, forming a bivalent that contrasts sharply with the completely synapsed autosomes. During pachytene, the XY bivalent becomes progressively shortened and more compact, disappearing as a visible structure when pachytene progresses into the diffuse stage of male meiosis. Diplotene bivalents gradually emerge from the diffuse nuclei, presumably by the return of the loops of chromatin into their respective chromomeres. During early diplotene, the X/Y bivalent is clearly visible with a single chiasma within the synapsed segment. This chiasma is terminalized by first meiotic metaphase with the X and Y appearing either in end-to-end synaptic contact or as univalents separated at opposite poles relative to the equatorially distributed autosomal bivalents. In C-banded preparations, the Y is entirely heterochromatic while the X contains a large centromeric C-band and another block of heterochromatin located at the telomeric end, in the region of synapsis with the Y. We find no cytological evidence of dosage compensation, such as differential staining of the X chromosomes or Barr bodies, in mitotic or interphase cells from female animals. 相似文献
3.
A chromosome complement formed by 16 autosomes and an Xyp sex chromosome system was found in Epilachna paenulata Germar (Coleoptera: Coccinellidae). All autosomes were metacentric except pair 1 which was submetacentric. The X and the Y chromosomes were also submetacentric but the Y was minute. The whole chromosome set carried large paracentric heterochromatic C-segments representing about 15% of the haploid complement length. Heterochromatic segments associated progressively during early meiotic stages forming a large single chromocenter. After C-banding, chromocenters revealed an inner networklike filamentous structure. Starlike chromosome configurations resulted from the attachment of bivalents to the chromocenters. These associations were followed until early diakinesis. Thin remnant filaments were also observed connecting metaphase I chromosomes. Evidence is presented that, in this species, the Xyp bivalent resulted from an end-to-end association of the long arms of the sex chromosomes. The parachute Xyp bivalent appeared to be composed of three distinct segments: two intensely heterochromatic C-banded corpuscles formed the canopy and a V-shaped euchromatic filament connecting them represented the parachutist component. The triple constitution of the sex bivalent was interpreted as follows: each heterochromatic corpuscle corresponded to the paracentric C-segment of the X and Y chromosomes; the euchromatic filament represented mainly the long arm of the X chromosome terminally associated with the long arm of the Y chromosome. The complete sequence of the formation of the Xyp bivalent starting from nonassociated sex chromosomes in early meiotic stages, and progressing through pairing of heterochromatic segments, coiling of the euchromatic filament, and movement of the heterochromatic corpuscles to opposite poles is described. These findings suggest that in E. paenulata the Xyp sex bivalent formation is different than in other coleopteran species and that constitutive heterochromatic segments play an important role not only in chromosome associations but also in the Xyp formation. 相似文献
4.
Garry T. Morgan 《Chromosoma》1978,66(3):269-280
Analysis of squash preparations of spermatocytes from crested newts, Triturus cristatus carnifex, has shown that in most cells at least one large bivalent regularly fails to form chiasmata in one arm-pair. Feulgen microphotometry of diplotene and metaphase bivalents has shown that it is the largest bivalent in each cell which shows chiasma failure in one arm-pair. A C-banding technique which identifies chromosome I by virtue of a long, darkly stained region in its long arm, was used to confirm the absence of chiasmata from one arm-pair of the longest bivalent, and specifically from the darkly stained region. The achiasmate region which chromosome I exhibits during spermatogenesis, corresponds to the heteromorphic region of oocyte lampbrush bivalent I in which chiasmata never form. A possible correlation between the complete absence of crossing-over from the heteromorphic region and unusual cytological and molecular features which it exhibits, are discussed. 相似文献
5.
B. Ringelhann J. G. Szelenyi M. Horanyi M. Svobodova V. Divoky K. Indrak S. Hollân A. Marosi M. Laub T. H. J. Huisman 《Human genetics》1993,92(4):385-387
We have identified seven different -thalassemia mutations and one -thalassemia determinant (the Sicilian type) in 32 members of 17 Hungarian families. The most common mutation is the IVS-I-1 (GA) change; its high frequency is comparable to that observed in neighboring Czechoslovakia. Additional mutations are of Mediterranean origin. One rare mutation (initiation codonATGGTG) was identified as an independent mutation because of the absence of known polymorphisms in the -globin gene. One new frameshift at codon 51 (-C) was observed in a single individual; hematological data were as expected for a °-thalassemia heterozygosity. 相似文献
6.
A hot spot of meiotic recombination has been found in males on murine chromosome 8 using nonisotopic hybridization of a series of probes to mitotic and meiotic chromosomes. The sequences responsible for this enhanced recombination are the telomeric repeats. Mice both normal and hetero- or homozygous for a pericentric inversion, In(8) 1 Rl, were analyzed. The inversion subdivides chromosome 8 into three discreet regions: (1) a fraction of the micro short arm that contains 30–150 kb of telomeric sequences and only about one-fifth of the contiguous minor-satellite sequences (approximately 200 kb); (2) the inverted region; and (3) the noninverted distal two-thirds of the chromosome. In 70 spermatocytes from inversion heterozygotes, examined by electron microscopy, synapsis of the inverted region was complete but entirely nonhomologous. Nonhomologous synapsis persists from initiation of synaptonemal complex formation in zygonema/early pachynema until dissolution in late pachynema. This nonhomologous synapsis also suppresses crossing over within the inverted segment. The opportunity for proximal homologous recombination is thus restricted to the roughly 250 kb segment located between the short-arm break and the end of the bivalent. Nonetheless, an extreme proximal chiasma was observed in 11% of the heterozygous chromosome-8 bivalents, 34% of the normal 8 bivalents and 35% of the homozygous inversion 8 bivalents from spermatocyte preparations. Since in the normal chromosomes all minor satellite sequences are adjacent to the telomere, while in the inversion chromosomes most of these sequences are transposed to an interstitial position without a corresponding shift in chiasma position, the minor-satellite sequences can be ruled out as promoters of recombination. Instead, the data suggest that it is the telomeric sequences that promote recombination, not just within the telomeric repeat itself, but quite frequently in sequences more than 250 kb away.by T. HassoldThis paper is dedicated to the memory of Barbara McClintock, whose early contributions on meiosis were as fundamental as her later ones on transposable elements. 相似文献
7.
J. Sybenga 《Genetica》1965,36(1):243-252
Whilst reliable estimates of chiasma frequencies can usually not be obtained, the probability (b) of a chromosome arm to be bound by at least one chiasma can often be determined. In the absence of interference this probability equals (1–e
–2), where 2 is the average chiasma frequency of the chromosome arm and the average crossover frequency or map length. In the presence of interference is shown to retain its genetic meaning as an additive metric that may describe the chromosome arm or other distinctive chromosome segment in terms of genetic recombination. It is a form of potential map length, comparable to, but numerically different from the regular map length. It is termed provisionally crossing-over potential.A chromosome with armsm andn with crossing-over potentials and will form ring bivalents with a frequency (1–e
–2).(1–e
–2); open bivalents with a frequency (1–e
–2).e
–2+(1–e
–2).e
–2; univalent pairs with a frequencye
–2.e
–2. Estimates of these frequencies yield equations from which and may be solved. In rye (Secale cereale) their ratio (q) is approximately two and differs from the mitotic arm length ratio of 1.4, indicating localization of chiasmata in the long arms.Graphs are given to show how, with constantq, the relation between the probabilitiesb
m
andb
n
of the two arms being bound changes with changing averageb.Data are presented on chiasma frequencies in M I, and compared with the frequencies expected in the absence of interference to give an impression of the degree of interference. Apparent fusion of chiasmata simulates interference. 相似文献
8.
Marina Bellani Veronica Shlain Clara Nudel Carmen Sanchez-Rivas 《Biotechnology letters》1997,19(1):1-5
Tn1000 (or ) insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested. 相似文献
9.
J. Sybenga 《Genetica》1965,36(1):351-361
The estimation of the crossing-over potentials of the two arms of a specific chromosome that can not be recognized in the diploid, was earlier found to be inefficient with the use of the primary trisomic. With the telocentric trisomics two groups of two different configurations each can be recognized that permit a reasonably exact estimation of the two parameters. Each telocentric trisomic yields estimates for both arms. The trisomic arm is underestimated as a result of partner-exchange and/or interference by the nucleolus in a nucleolus bearing arm. The other arm is estimated more correctly. Thus the two telocentrics together give a complete picture of the chromosome. After a correction for differences in overall chiasma frequencies the ratio of the crossing-over potentials of the two arms of the satellite chromosome ofSecale cereale was found to be approximately 2. This is large for a submedian chromosome in comparison with the ratio for the genome as a whole and it is attributed tentatively to the nucleolus interfering with chiasma formation in the short arm. It is suggested that the three homologous arms, especially the long arms, differ in respect to the tendency to pair and in chiasma frequency. 相似文献
10.
Chromosome pairing and chiasma frequency were studied in meiocytes at diakinesis of Lilium speciosum cv. Rosemede fixed up to 21 days after the start of either continuous or 3 day pulse colchicine treatment. The two treatments gave similar results. In pulse treated pollen mother cells (PMCs) the mean chiasma frequency per cell fell from 26.4 in controls to 8.5 after fourteen days while the mean number of univalents per cell increased from 0.05 to 17.58. There was a negative correlation between mean chiasma frequency per bivalent and per PMC in colchicine treated buds; univalents were preferentially induced in bivalents with one chiasma, and preferentially excluded in bivalents with 4 chiasmata. Some chiasmata were redistributed to surviving bivalents despite the concurrent reduction in chiasma frequency per meiocyte. — Colchicine sensitivity began in premeiotic interphase and extended to mid or late zygotene in PMCs; ongoing synapsis was unaffected. However, susceptibility to univalency was asynchronous between bivalents occurring at zygotene in short chromosomes but at late premeiotic interphase in the longest chromosomes. The number of chiasmata per bivalent could be altered by colchicine without inducing univalents, but the ultimate effect was to reduce the number of chiasmata per bivalent (or per chromosome arm) directly to zero. The major factors determining the order and extent of reduced pairing and chiasma number were total chromosome length and arm length. Pairing and chiasma formation in embryo sac mother cells were less sensitive to colchicine than in PMCs, but their behavior was otherwise similar. 相似文献
11.
Ivor P. B. Tittawella 《Molecular & general genetics : MGG》1985,200(1):114-117
Summary A mutation located near rpoB (89) in E. coli is responsible for unusual patterns of and (but not L7/L12) synthesis in response to the drugs rifampicin and acridine orange. 相似文献
12.
Roberto Gambino Gianluca Ruiu Gianfranco Pagano Maurizio Cassader 《Journal of Protein Chemistry》1997,16(3):205-212
The specific binding of digoxigenin-labeled lectins to carbohydrate moieties is used to characterize the carbohydrate chains bound to apolipoprotein H. Our results show that apolipoprotein H is rich in sialic acid linked (2–6) to galactose or N-acetylgalactosamine. Sialic acid is not (2–3)-linked to galactose. Galactose is (1–4)-linked to N-acetylglucosamine and (1–3)-linked to N-acetylgalactosamine. High-mannose N-glycan chains are barely detectable. After N-glycosidase F treatment the molecular weight is substantially reduced. The main band is 32,500 daltons. Carbohydrate O-linked chains, which are mainly represented by sialic acid, are (2–6)-linked to galactose or N-acetylgalactosamine. Galactose is also organized in O-linked chains and it is (1–4)-linked to N-acetylglucosamine and (1–3)-linked to acetylgalactosamine. Biochemical analysis of carbohydrate structures reveals that no specific carbohydrate complex is bound to a single isoform. 相似文献
13.
G. Jenkins 《Chromosoma》1985,92(2):81-88
The chromosomes of Lolium temulentum are longer and contain on average 50% more nuclear DNA than the chromosomes of L. perenne. In the hybrid, despite the difference in length and DNA content, pairing between the homoeologous chromosomes at pachytene is effective and the chiasma frequency at first metaphase in pollen mother cells is high, about 1.6 per bivalent, comparable to that in the L. perenne parent. Electron microscopic observations from reconstructed nuclei at pachytene show that synaptonemal complex (SC) formation in 40% of bivalents is perfect, complete and continuous from telomere to telomere. In others, SCs extend from telomere to telomere but incorporate lateral component loops in interstitial chromosome segments. Even in these bivalents, however, pairing is effective in the sense of chiasma formation. The capacity to form perfect SCs is achieved by an adjustment of chromosome length differences both before and during synapsis. Perfect pairing and SC formation is commoner within the larger bivalents of the complement. At zygotene, in contrast to pachytene, pairing is not confined to homoeologous chromosomes. On the contrary there is illegitimate pairing between non-homologous chromsomes resulting in multivalent formation. There must, therefore, be a mechanism operative between zygotene and pachytene that corrects and modifies associations in such a way as to restrict the pairing to bivalents comprised of strictly homoeologous chromosomes. Such a correction bears comparison with that known to apply in allopolyploids. In the hybrid and in the L. perenne parent also, certain specific nucleolar organisers are inactivated at meiosis. 相似文献
14.
Female-specific features of recombinational double-stranded DNA repair in relation to synapsis and telomere dynamics in human oocytes 总被引:1,自引:0,他引:1
Chromosome segregation errors are a significant cause of aneuploidy among human neonates and often result from errors in female meiosis that occur during fetal life. For the latter reason, little is known about chromosome dynamics during female prophase I. Here, we analyzed chromosome reorganization, and centromere and telomere dynamics in meiosis in the human female by immunofluorescent staining of the SYCP3 and SYCP1 synaptonemal complex proteins and the course of recombinational DNA repair by IF of phospho-histone H2A.X (-H2AX), RPA and MLH1 recombination proteins. We found that SYCP3, but not SYCP1, aggregates appear in the preleptotene nucleus and some persist up to pachytene. Telomere clustering (bouquet stage) in oocytes lasted from late-leptotene to early pachytene—significantly longer than in the male. Leptotene and zygotene oocytes and spermatocytes showed strong -H2AX labeling, while -H2AX patches, which colocalized with RPA, were present on SYCP1-tagged pachytene SCs. This was rarely seen in the male and may suggest that synapsis installs faster with respect to progression of recombinational double-strand break repair or that the latter is slower in the female. It is speculated that the presence of -H2AX into pachytene highlights female-specific peculiarities of recombination, chromosome behavior and checkpoint control that may contribute to female susceptibility for aneuploidy.I. Roig and B. Liebe made an equal contribution to this work 相似文献
15.
Heterotrimeric GTP-binding proteins, composed of , , and subunits, are involved in signal transduction pathways in animal and plant systems. In plants, physiological analyses implicate heterotrimeric G-proteins in ion channel regulation, light signaling, and hormone and pathogen responses. However, only one class of plant G genes has been identified to date. We have cloned a novel gene, Arabidopsis thaliana extra-large GTP-binding protein (AtXLG1). AtXLG1 appears to be a member of a small gene family and is transcribed in all tissues assayed: roots, leaves, stems, flowers, and fruits. The conceptually translated protein from AtXLG1 is 99 kDa, twice as large as typical G proteins. The carboxy-terminal half of the AtXLG1 protein has significant homology to animal and plant G proteins. This region includes a GTP-binding domain, a predicted helical domain, and an aspartate/glutamate-rich loop, which are characteristics of G's. Despite the absence of some of the amino acids implicated in GTP binding and hydrolysis by crystallographic and mutational analyses of mammalian G's, recombinant AtXLGl binds GTP with specificity. The amino-terminal region of AtXLGl contains domains homologous to the bacterial TonB-box, which is involved in energy transduction between the inner and outer bacterial membranes, and to zinc-finger proteins. Given the unique structure of AtXLG1, it will be of interest to uncover its physiological functions. 相似文献
16.
A surface-spreading synaptonemal complex (SC) technique was employed to analyze spermatocytes and oocytes of stickleback, Gasterosteus aculeatus, in order to visualize the process of chromosome synapsis. The mean SC length was 150±18m in three males and 143±12m in one female analyzed. A representative SC karyotype with 21bivalents was also presented. Each SC had lateral elements of equal length. No bivalent displaying the atypical synaptic behaviour which is often associated with heteromorphic sex chromosomes was observed neither in males nor in the female analyzed. 相似文献
17.
Chiasma distribution in Truxaline grasshoppers 总被引:3,自引:1,他引:2
David I. Southern 《Chromosoma》1967,22(2):164-191
Similar patterns of chiasma distribution are found within the individual arms of the chromosome complement in four species of Truxaline grasshopper. There is a linear relationship between chiasma frequency and chromosome arm length although the telocentric elements have a consistently higher mean number of chiasmata per unit of arm length. The positions of successive chiasmata can be defined in terms of residual (r.c. and r.t.) and interference (T) distances which vary in value according to both arm length and chiasma frequency. There is a tendency for one chiasma to lie in a distal position which is accentuated when additional chiasmata form. Supernumerary B chromosomes do not appear to influence the overall control mechanism of chiasma distribution. There is no indication that bivalents within a nucleus compete for chiasmata nor does the chiasma distribution in one arm of the metacentric members influence that in the other. It is suggested that the control of chiasma formation is determined mainly by interference factors. 相似文献
18.
Two South African Pyrgomorpha species have reduced chromosome numbers, due to centric fusions between the largest autosomes and the medium and small autosomes. P. rugosa has 2n=11(XO) (4 pairs of submetacentric and 1 pair of acrocentric autosomes) and P. granulata has 2n=13(XO) (3 pairs of submetacentric and 3 pairs of acrocentric autosomes). A third South African species has a typical Pyrgomorphidae number of 2n=19(XO) (acrocentrics). The mean chiasma frequency of the 2n=19 species is higher than that of the other two, although the frequencies of distal chiasmata in all three are similar. The recombination potential of the two species with lower chromosome numbers has been reduced, due to fewer crossovers in comparison to the 2n=19 species, as well as to independent assortment. 相似文献
19.
The chromosomes of the caddis-fly,Glyphotaelius pellucidus (Retzius, 1783) (Trichoptera: Limnephilidae,limnephilinae) 总被引:1,自引:1,他引:0
The chromosome number in the caddis-fly,Glyphotaelius pellucidus (Retz.) is: 2n =60, =59; n =30, =29, 30. The sex determining mechanism is XX-XO, the female being the heterogametic sex. The sex element is heteropycnotic at oogonial prometaphase and at oocyte pachytene. There are no chiasmata at oocyte diakinesis, whereas one or two of these occur per bivalent in the male. The smallest bivalent has a delayed pairing at spermatocyte diakinesis. 相似文献
20.
Genetic and environmental components of chiasma control 总被引:1,自引:0,他引:1
D. D. Shaw 《Chromosoma》1972,37(3):297-308
A disruptive selection programme for high and low chiasma frequency over four generations has produced two lines of Schistocerca gregaria with mean values of 21.6 and 18.0, respectively. The mean of the base population was 20.4. Realised heritability estimates for both the high and low lines were 27.4% and 48.8% respectively; only the latter value is significant. The non-significant value in the high line has resulted either from unidirectional dominance or because the genes responsible for high chiasma values themselves undergo a higher recombination rate in high frequency individuals and thus reduce the response to selection. — It is suggested that the genes governing chiasma frequency can be regarded as mainly neutral due to the large additive genetic component, at least in low chiasma frequency types. Accordingly, chiasma frequency variation and its adaptive significance may not be such an important component of fitness as has been proposed in the past. 相似文献