共查询到20条相似文献,搜索用时 375 毫秒
1.
2.
Jill Deikman 《Physiologia plantarum》1997,100(3):561-566
3.
4.
Yamamoto S Suzuki K Shinshi H 《The Plant journal : for cell and molecular biology》1999,20(5):571-579
5.
A Nitrilase-Like Protein Interacts with GCC Box
DNA-Binding
Proteins Involved in Ethylene and
Defense Responses 总被引:5,自引:1,他引:4
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ping Xu Meena L. Narasimhan Teresa Samson Maria A. Coca Gyung-Hye Huh Jianmin Zhou Gregory B. Martin Paul M. Hasegawa Ray A. Bressan 《Plant physiology》1998,118(3):867-874
Ethylene-responsive element-binding proteins (EREBPs) of tobacco (Nicotiana tabacum L.) bind to the GCC box of many pathogenesis-related (PR) gene promoters, including osmotin (PR-5). The two GCC boxes on the osmotin promoter are known to be required, but not sufficient, for maximal ethylene responsiveness. EREBPs participate in the signal transduction pathway leading from exogenous ethylene application and pathogen infection to PR gene induction. In this study EREBP3 was used as bait in a yeast two-hybrid interaction trap with a tobacco cDNA library as prey to isolate signal transduction pathway intermediates that interact with EREBPs. One of the strongest interactors was found to encode a nitrilase-like protein (NLP). Nitrilase is an enzyme involved in auxin biosynthesis. NLP interacted with other EREBP family members, namely tobacco EREBP2 and tomato (Lycopersicon esculentum L.) Pti4/5/6. The EREBP2-EREBP3 interaction with NLP required part of the DNA-binding domain. The specificity of interaction was further confirmed by protein-binding studies in solution. We propose that the EREBP-NLP interaction serves to regulate PR gene expression by sequestration of EREBPs in the cytoplasm. 相似文献
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.