The influence of a congested calendar on physical performance in elite soccer

The aim of this study was to investigate recovery via analysis of activity profiles in a professional soccer team over an intense period of matches. A total of 172 outfield players from 27 Spanish League matches played by a professional team during the 2005-2006 season were analyzed using a multiple-camera match analysis system. The dependent variables were the distance covered by players at different intensities. Data were analyzed using an independent-sample t-test and a linear regression analysis with 5 independent variables: the number of matches played per week (1 or 2), match status (i.e., whether the team was winning, losing or drawing), match location (i.e., playing at home or away), quality of the opponents (strong or weak), and the individual playing position of the players. The main finding of this study suggests that the activity profiles of professional soccer players were not influenced by short recovery between matches. Although those players who played 2 matches a week covered less distance at maximal (>23 km·h(-1)), submaximal (19.1-23 km·h(-1)), and medium (14.1-19 km·h(-1)) intensities than those players who played 1 match a week, no significant differences were found. Moreover, results from this study seem to confirm that the elite soccer players' distance covered at various speeds is dependent on match contextual factors. The top-class players performed less high-intensity activity (>19.1 km·h(-1)) when winning than when they were losing (p < 0.05), but more distance was covered by walking and jogging when winning (p < 0.05). The home teams covered a greater distance than away teams at low intensity (<14.1 km·h(-1)) (p < 0.01). Finally, the better the quality of the opponent, the higher the distance covered by walking and jogging.     

Small-sided games in soccer: amateur vs. professional players' physiological responses, physical, and technical activities

The aim of this study was to examine the relationship between the playing level in soccer (i.e., amateur vs. professional players) and the physiological impact, perceptual responses, time-motion characteristics, and technical activities during various small-sided games (SSGs). Twenty international players (27.4 ± 1.5 years and 17.4 ± 0.8 km·h(-1) of vVO(2)max) and 20 amateur players of the fourth French division (26.3 ± 2.2 years and 17.0 ± 1.2 km·h(-1) of vVO(2)max) played 9 SSGs (i.e., 2 vs. 2, 3 vs. 3, and 4 vs. 4) in which the number of ball touches authorized by possession varied (1 ball touch authorized = 1T, 2 ball touches authorized = 2T, and Free Play = FP). Heart rate (HR), blood lactate ([La]), subjective perception of effort (rating of perceived exertion [RPE]), physical performance, and technical performance of all players were analyzed during all SSGs. Across the various SSGs, amateurs completed a lower percent of successful passes (p < 0.01), recorded higher RPE and [La] values, lost a greater amount of ball possessions (p < 0.001), and covered less total distance with respect to sprinting and high-intensity running (HIR). The HR responses, however, were similar when expressed as %HRmax and %HRreserve. The comparison of the professional and amateur soccer players' activities during SSGs showed that the playing level influenced the physiological responses, physical and technical activities. Consequently, this study has shown that the main differences between elite and amateur players within SSGs concerned their capacity to perform high-intensity actions (HIR and sprints) and execute various technical abilities (in particular number of ball lost per possession and percentage of successful passes).     

Match activities of elite women soccer players at different performance levels

We sought to study the physical demands and match performance of women soccer players. Nineteen top-class and 15 high-level players were individually videotaped in competitive matches, and time-motion analysis were performed. The players changed locomotor activity >1,300 times in a game corresponding to every ~4 seconds and covered 9-11 km in total. The top-class players ran 28% longer (P < 0.05) at high intensities than high-level players (1.68 +/- 0.09 and 1.33 +/- 0.10 km, respectively) and sprinted 24% longer (P < 0.05). The top-class group had a decrease (P < 0.05) of 25-57% in high intensity running in the final 15 minutes compared with the first four 15-minutes intervals, whereas the high-level group performed less (P < 0.05) high-intensity running in the last 15 minutes of each half in comparison with the 2 previous 15-minute periods in the respective half. Peak distance covered by high intensity running in a 5-minute interval was 33% longer (P < 0.05) for the top-class players than the high-level players. In the following 5 minutes immediately after the peak interval top-class players covered 17% less (P < 0.05) high-intensity running than the game average. Defenders performed fewer (P < 0.05) intervals of high-intensity running than midfielders and attackers, as well as fewer (P < 0.05) sprints than the attackers. In conclusion, for women soccer players (1) top-class international players perform more intervals of high-intensity running than elite players at a lower level, (2) fatigue develops temporarily during and towards the end of a game, and (3) defenders have lower work rates than midfielders and attackers. The difference in high-intensity running between the 2 levels demonstrates the importance of intense intermittent exercise for match performance in women soccer. Thus, these aspects should be trained intensively in women soccer.     

Muscle damage, endocrine, and immune marker response to a soccer match

ABSTRACT: Thorpe, R and Sunderland, C. Muscle damage, endocrine, and immune marker response to a soccer match. J Strength Cond Res 26(10): 2783-2790, 2012-This study represents the first time that muscle damage, endocrine, and immune markers have been measured, together with activity profile, during a competitive soccer match. Seven semiprofessional soccer players participated in a competitive league match. Blood and saliva samples were obtained 1 hour before kick off and immediately postmatch. Global positioning system equipment was used to measure heart rate and activity profile data throughout the match. Percentage increase in creatine kinase (CK) and myoglobin (MYO) concentrations was correlated with the number of sprints performed during the match (r = 0.88, p = 0.019; r = 0.75, p = 0.047, respectively). Creatine kinase increased by 84% (p = 0.17) from prematch to postmatch, whereas MYO increased by 238% (p = 0.05). Players performed 39 ± 18 sprints during the course of the match. Cortisol increased by 78% (p = 0.103), whereas testosterone increased significantly by 44% (p = 0.004). No differences were seen from prematch to postmatch in the testosterone to cortisol ratio, immunoglobulin (Ig) A, IgM, or IgG. Sprinting is correlated with changes in CK and MYO and may therefore be associated with muscle damage in semiprofessional soccer players.     

Do compression garments enhance the active recovery process after high-intensity running?

Lovell, DI, Mason, DG, Delphinus, EM, and McLellan, CP. Do compression garments enhance the active recovery process after high-intensity running? J Strength Cond Res 25(12): 3264-3268, 2011-This study examined the effect of wearing waist-to-ankle compression garments (CGs) on active recovery after moderate- and high-intensity submaximal treadmill running. Twenty-five male semiprofessional rugby league players performed two 30-minute treadmill runs comprising of six 5-minute stages at 6 km·h, 10 km·h, approximately 85% VO(2)max, 6 km·h as a recovery stage followed by approximately 85% VO(2)max and 6 km·h wearing either CGs or regular running shorts in a randomized counterbalanced order with each person acting as his own control. All stages were followed by 30 seconds of rest during which a blood sample was collected to determine blood pH and blood lactate concentration [La]. Expired gases and heart rate (HR) were measured during the submaximal treadmill tests to determine metabolic variables with the average of the last 2 minutes used for data analysis. The HR and [La] were lower (p ≤ 0.05) after the first and second 6 km·h recovery bouts when wearing CGs compared with when wearing running shorts. The respiratory exchange ratio (RER) was higher and [La] lower (p ≤ 0.05) after the 10 km·h stage, and only RER was higher after both 85% VO(2)max stages when wearing CGs compared with when wearing running shorts. There was no difference in blood pH at any exercise stage when wearing the CGs and running shorts. The results of this study indicate that the wearing of CGs may augment the active recovery process in reducing [La] and HR after high-intensity exercise but not effect blood pH. The ability to reduce [La] and HR has important consequences for many sports that are intermittent in nature and consist of repeated bouts of high-intensity exercise interspersed with periods of low-intensity exercise or recovery.     

Effect of training intensity distribution on aerobic fitness variables in elite soccer players: a case study

The aim of this article was to quantify the distribution of training intensities and its effect on aerobic fitness in professional elite soccer players. Fourteen professional soccer players were observed during the prechampionship training period (6 weeks). Treadmill running speed and heart rates (HRs) at 2 and 4 mmol · L(-1) blood-lactate concentrations were assessed pre and posttraining. Training intensities were categorized using 3 HR zones: low intensity (

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HR 4 mmol · L(-1)). Analysis of the 504 individual training sessions showed that 73 ± 2.5, 19 ± 2.8, and 8 ± 1.4% of the total training time was spent at low, moderate, and high intensity, respectively (p < 0.001). Speed at 2 and 4 mmol · L(-1) significantly improved posttraining (5 and 7%, respectively, p < 0.01). Training spent at high intensity was significantly related to relative speed improvements at 2 mmol · L(-1) (r = 0.84, p < 0.001;) and 4 mmol · L(-1) (r = 0.65, p = 0.001). Players spent almost two-thirds of their training time at low intensities. However, only the time spent at high intensity (>90% of maximal HR) was related to changes in aerobic fitness. These results support the usefulness of the quantification of aerobic training load using HR. Furthermore, it stresses the effectiveness of the high-intensity training in soccer.     

Physiological responses and time-motion characteristics of 4-a-side small-sided game in young soccer players: the influence of different team formation methods

ABSTRACT: K?klü, Y, Ers?z, G, Alemdaro?lu, U, A???, A, and ?zkan, A. Physiological responses and time-motion characteristics of 4-A-side small-sided game in young soccer players: The influence of different team formation methods. J Strength Cond Res 26(11): 3118-3123, 2012-The purpose of this study was to examine the influence of different team formation methods on the physiological responses to and time-motion characteristics of 4-a-side small-sided games (SSG4) in young soccer players. Thirty-two young soccer players (age 16.2 ± 0.7 years; height 172.9 ± 6.1 cm; body mass 64.1 ± 7.7 kg) voluntarily participated in this study. Anthropometric measurements, technical tests, and maximum oxygen uptake (V[Combining Dot Above]O2max) tests were carried out on the players. The SSG4 teams were then created using 4 different methods: according to the coaches' subjective evaluation (CE), technical scores (TS), V[Combining Dot Above]O2max (AP), and V[Combining Dot Above]O2max multiplied by TSs (CG). The teams thus created played 4 bouts of SSG4 at 2-day intervals. During the SSG4, heart rate (HR) responses, distance covered, and time spent in HRmax zones were recorded. In addition, rating of perceived exertion (RPE) and blood lactate level (La) were determined at the end of the last bout of each SSG4. Percent of HRmax (%HRmax), La, and RPE responses during SSG4 were significantly higher for teams chosen according to AP and CG compared with that according to CE and TS (p < 0.05). In addition, teams chosen by AP and CG spent significantly more time in zone 4 (>90% HRmax ) and covered a greater distance in the high-intensity running zone (>18 km·h) than did teams formed according to TS. Moreover, AP teams covered significantly greater total distance than TS teams did (p < 0.05). In conclusion, to spend more time in both the high-intensity HR zone and the high-intensity running zone, the teams in SSG4 should be formed according to the players' V[Combining Dot Above]O2max values or the values calculated using both the V[Combining Dot Above]O2max and technique scores.     

Time-motion analysis of small-sided training games and competition in elite women soccer players

We investigated the movement patterns of small-sided training games and compared these movement patterns with domestic, national, and international standard competition in elite women soccer players. In addition, we investigated the repeated-sprint demands of women's soccer with respect to the duration of sprints, number of sprint repetitions, recovery duration, and recovery intensity. Thirteen elite women soccer players [age (mean +/- SD) 21 +/- 2 years] participated in this study. Time-motion analysis was completed during training (n = 39) consisting of small-sided (i.e., three versus three and five versus five) training games, domestic matches against male youth teams (n = 10), Australian national-league matches (n = 9), and international matches (n = 12). A repeated-sprint bout was defined as a minimum of three sprints, with recovery of less than 21 seconds between sprints. The overall exercise to rest ratios for small-sided training games (1:13) were similar to or greater than domestic competition against male youth teams (1:15) and national-league (1:16) and international (1:12) competitions. During the international matches analyzed, 4.8 +/- 2.8 repeated-sprint bouts occurred per player, per match. The number of sprints within the repeated-sprint bouts was 3.4 +/- 0.8. The sprint duration was 2.1 +/- 0.7 seconds, and the recovery time between sprints was 5.8 +/- 4.0 seconds. Most recovery between sprints was active in nature (92.6%). In contrast to international competition, repeated-sprint bouts were uncommon in small-sided training games, domestic competition against male youth teams, and national-league competition. These findings demonstrate that small-sided training games simulate the overall movement patterns of women's soccer competition but offer an insufficient training stimulus to simulate the high-intensity, repeated-sprint demands of international competition.     

The validity and reliability of 5-Hz global positioning system units to measure team sport movement demands

The purpose of this research was to investigate the validity and the reliability of 5-Hz MinimaxX global positioning system (GPS) units measuring athlete movement demands. A team sport simulation circuit (files collected from each unit = 12) and flying 50-m sprints (files collected from each unit = 34) were undertaken, during which the total distance covered; peak speed; player load; the distance covered; time spent and number of efforts performed walking, jogging, running, high-speed running, and sprinting were examined. Movement demands were also separately categorized into low-intensity activity, high-intensity running, and very high-intensity running. The results revealed that GPS was a valid and reliable measure of total distance covered (p > 0.05, percentage typical error of measurement [%TEM] < 5%) and peak speed (p > 0.05, %TEM 5-10%). Further, GPS was found to be a reliable measure of player load (%TEM 4.9%) and the distance covered, time spent, and number of efforts performed at certain velocity zones (%TEM <5% to >10%). The level of GPS error was found to increase along with the velocity of exercise. The findings demonstrated that GPS is capable of measuring movement demands performed at velocities <20 km·h(-1), whereas more caution is to be exercised when analyzing movement demands collected by using GPS velocities >20 km·h(-1).     

Intermatch variation of match activity in elite Italian soccer referees

The aim of this study was to examine how match activities, specifically high-intensity activity (HIA; activities performed at speeds faster than 18.1 km x h(-1)), vary among matches in elite soccer referees, because variations in match performance can potentially guide physical conditioning regimens for these referees. Fourteen Italian soccer referees were observed during 65 first-division matches a minimum of 3 and a maximum of 6 times each. For comparison, shorter matches (SM) and longer matches (LM) for each referee were identified. Mean (+/-SD) SM and LM total distances were 10,949 +/- 1,095 m and 12,303 +/- 666 m (p < 0.001), respectively. SM and LM were not different in term of duration (p > 0.05). During LM, referees spent 44% less time standing still (p < 0.01) and covered 10% more distance at low intensity than they did in SM (p < 0.05). No HIA variation was observed between SM and LM (p > 0.05). For the elite referee, work rate is increased without affecting HIA.     

Effects of a caffeine-containing energy drink on simulated soccer performance

Background

To investigate the effects of a caffeine-containing energy drink on soccer performance during a simulated game. A second purpose was to assess the post-exercise urine caffeine concentration derived from the energy drink intake.

Methodology/Principal Findings

Nineteen semiprofessional soccer players ingested 630±52 mL of a commercially available energy drink (sugar-free Red Bull®) to provide 3 mg of caffeine per kg of body mass, or a decaffeinated control drink (0 mg/kg). After sixty minutes they performed a 15-s maximal jump test, a repeated sprint test (7×30 m; 30 s of active recovery) and played a simulated soccer game. Individual running distance and speed during the game were measured using global positioning satellite (GPS) devices. In comparison to the control drink, the ingestion of the energy drink increased mean jump height in the jump test (34.7±4.7 v 35.8±5.5 cm; P<0.05), mean running speed during the sprint test (25.6±2.1 v 26.3±1.8 km · h⁻¹; P<0.05) and total distance covered at a speed higher than 13 km · h⁻¹ during the game (1205±289 v 1436±326 m; P<0.05). In addition, the energy drink increased the number of sprints during the whole game (30±10 v 24±8; P<0.05). Post-exercise urine caffeine concentration was higher after the energy drink than after the control drink (4.1±1.0 v 0.1±0.1 µg · mL⁻¹; P<0.05).

Conclusions/significance

A caffeine-containing energy drink in a dose equivalent to 3 mg/kg increased the ability to repeatedly sprint and the distance covered at high intensity during a simulated soccer game. In addition, the caffeinated energy drink increased jump height which may represent a meaningful improvement for headers or when players are competing for a ball.     

Validity of Carminatti's test to determine physiological indices of aerobic power and capacity in soccer and futsal players

The aim of this study was to verify the validity of a new progressive distance and fixed time test (Carminatti's test [TCAR]) in estimating the main physiological indices of aerobic fitness in team-sport players. Thirty professional national level team-sport players (n = 12 futsal players and 18 soccer players) volunteered to participate in this study. The subjects performed the TCAR and a laboratory incremental treadmill test (ITT). The TCAR required subjects to complete repeated sets of 5 × 12-second shuttle-running bouts at progressive speed until volitional exhaustion. Each 12-second bout and series were separated by a 6- and 90-second recovery periods, respectively. The initial distance was set at 15 m and was progressively increased by 1 m each set. The ITT commenced at a velocity of 9.0 km·h(-1) and was increased by 1.2 km·h(-1) each 3 minutes until volitional exhaustion. Peak TCAR running velocity resulted not significantly (p > 0.05) different from speed at VO2max (vVO2max) during ITT. Peak TCAR running velocity was significantly correlated (p < 0.01) with vVO2max (r = 0.55) and VO2max (r = 0.51). No significant differences were found (p > 0.05) among the mean values of velocity and heart rate at the anaerobic threshold, estimated in the TCAR test and measured in the ITT. In light of this study results, the TCAR can be considered as a viable field test to estimate aerobic power and capacity in team-sports players. The limited devices and space required by TCAR warrant consideration for those strength and conditioning professionals who deal with team sports.     

Changes in Acid-base balance during simulated soccer match play

This study evaluated changes in markers of acid-base balance that occurred during simulated soccer match play. Sixteen academy soccer players participated in a soccer match simulation that consisted of 90 minutes of soccer-specific exercise with skills throughout. Blood samples were obtained before exercise (preexercise), every 15 minutes during the simulation (15, 30, 45, 60, 75, and 90 minutes), and 10 minutes into the half-time break (half time). Blood lactate concentrations were elevated throughout exercise (preexercise: 1.5 ± 0.12 mmol·L; 90 minutes: 6.1 ± 0.7 mmol·L, time effect: p < 0.01, partial-eta = 0.740). Relative to preexercise values, actual blood bicarbonate (preexercise: 28.02 ± 0.92 mmol·L; 90 minutes: 21.73 ± 0.65 mmol·L, time effect = p < 0.01, partial-eta = 0.680), standard blood bicarbonate (preexercise: 25.97 ± 0.43 mmol·L; 90 minutes: 22.87 ± 0.31 mmol·L, time effect = p < 0.01, partial-eta = 0.667), base excess (preexercise: 2.40 ± 0.54 mmol·L, 90 minutes: -1.57 ± 0.39 mmol·L, time effect = p < 0.01, partial-eta = 0.664), and pH (preexercise: 7.44 ± 0.01 units; 90 minutes: 7.39 ± 0.01 units, time effect = p < 0.01, partial-eta = 0.542) were depressed throughout the exercise. Interestingly, blood bicarbonate, base excess, and pH recovered at half time (p > 0.05). This is the first study to provide data concerning the acid-base balance of familiarized soccer players during exercise that simulates soccer match play. These findings suggest that (a) blood pH is reduced during soccer-specific exercise and (b) although buffering capacity is reduced throughout exercise, it returns to normal during half time. Further research is warranted to develop interventions that can maintain acid-base balance throughout the full duration of a soccer match.     

The interchangeability of global positioning system and semiautomated video-based performance data during elite soccer match play

In elite-level soccer, player motion characteristics are commonly generated from match play and training situations using semiautomated video analysis systems and global positioning system (GPS) technology, respectively. Before such data are used collectively to quantify global player load, it is necessary to understand both the level of agreement and direction of bias between the systems so that specific interventions can be made based on the reported results. The aim of this report was to compare data derived from both systems for physical match performances. Six elite-level soccer players were analyzed during a competitive match using semiautomated video analysis (ProZone? [PZ]) and GPS (MinimaxX) simultaneously. Total distances (TDs), high speed running (HSR), very high speed running (VHSR), sprinting distance (SPR), and high-intensity running distance (HIR; >4.0 m·s(-1)) were reported in 15-minute match periods. The GPS reported higher values than PZ did for TD (GPS: 1,755.4 ± 245.4 m; PZ: 1,631.3 ± 239.5 m; p < 0.05); PZ reported higher values for SPR and HIR than GPS did (SPR: PZ, 34.1 ± 24.0 m; GPS: 20.3 ± 15.8 m; HIR: PZ, 368.1 ± 129.8 m; GPS: 317.0 ± 92.5 m; p < 0.05). Caution should be exercised when using match-load (PZ) and training-load (GPS) data interchangeably.     

Coordination of soccer players during preseason training

This study aimed to verify whether coordination improves as a result of a preseason soccer training. During 5 experimental sessions (days 1, 6, 11, 15, and 19), 16 semiprofessional male soccer players (22.0 ± 3.6 years) were administered 3 specific soccer tests (speed dribbling, shooting a dead ball, and shooting from a pass) and an interlimb coordination test (total duration of a trial: 60 seconds), consisting of isodirectional and nonisodirectional synchronized (1:1 ratio) hand and foot flexions and extensions at an increasing velocity of execution (80, 120, and 180 b·min(-1)). Furthermore, subjective ratings were monitored to assess the recovery state (RestQ) of the players, their perceived exertion (rating of perceived exertion [RPE]) for the whole body, and the perceived muscle pain (rating of muscle pain [RMP]) for the lower limbs and the internal training load by means of the session-RPE method. The ratios between post and pretraining RPE and RMP increased only during the first 2 experimental sessions and decreased after the second week of the training camp (p = 0.001). The Rest-Q showed increases (p < 0.05) for general stress, conflict/pressure, social recovery, and being in shape dimensions. Conversely, decreases (p < 0.05) were observed for social stress, fatigue, physical complaints dimensions. Throughout the preseason, the players improved their speed dribbling (p = 0.03), Shooting from a Pass (p = 0.02), and interlimb coordination (p < 0.0001) performances. These coordination tests succeeded in discriminating coordination in soccer players and could integrate field test batteries during the whole soccer season, because they were easily and inexpensively administrable by coaches.     

Physical responses of different small-sided game formats in elite youth soccer players

A major use of small-sided games (SSGs) in soccer training is the concomitant development of game-specific aerobic fitness. We hypothesize that the SSG formats of 2 vs. 2, 3 vs. 3, and 4 vs. 4 players reveal game-like intensities and therefore are most adequate to increase game-specific aerobic fitness. Heart rate (HR), percentage of maximum heart rate (HRmax), blood lactate concentration (La), and time-motion characteristics of 17 elite male youth soccer players (aged 14.9 ± 0.7 years, V[Combining Dot Above]O2max 61.4 ± 4.5 ml·kg·min, HRmax 199.6 ± 7.3 b·min) were collected by global positioning systems while performing the SSG formats. Repeated-measures analysis of variance and effect sizes were calculated to demonstrate the differences between SSG formats. Highest physiological responses were obtained in 2 vs. 2 (HR: 186 ± 7 b·min, HRmax: 93.3 ± 4.2%, La: 5.5 ± 2.4 mmol·L) followed by 3 vs. 3 (HR: 184 ± 8 b·min, HRmax: 91.5 ± 3.3%, La: 4.3 ± 1.7 mmol·L) and 4 vs. 4 (HR: 179 ± 7 b·min, HRmax 89.7 ± 3.4%, La: 4.4 ± 1.9 mmol·L). Pronounced differences were found for most physiological parameters and for time spent in the speed zones "walking" (<5.3 km·h), "moderate-speed running" (10.3-13.9 km·h), and "maximum sprinting" (≥26.8 km·h). The findings suggest that all the formats reveal game-like intensities and are suitable for aerobic fitness improvements. However, we found pronounced demands on the anaerobic energy supply in 2 vs. 2, whereas 3 vs. 3 and 4 vs. 4 remain predominantly on an aerobic level and differ mainly in the HR response. We suggest using 3 vs. 3 for soccer-specific aerobic fitness training.     

C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced by oxidative stress

Background

TDP-43 proteinopathies are characterized by loss of nuclear TDP-43 expression and formation of C-terminal TDP-43 fragmentation and accumulation in the cytoplasm. Recent studies have shown that TDP-43 can accumulate in RNA stress granules (SGs) in response to cell stresses and this could be associated with subsequent formation of TDP-43 ubiquinated protein aggregates. However, the initial mechanisms controlling endogenous TDP-43 accumulation in SGs during chronic disease are not understood. In this study we investigated the mechanism of TDP-43 processing and accumulation in SGs in SH-SY5Y neuronal-like cells exposed to chronic oxidative stress. Cell cultures were treated overnight with the mitochondrial inhibitor paraquat and examined for TDP-43 and SG processing.

Results

We found that mild stress induced by paraquat led to formation of TDP-43 and HuR-positive SGs, a proportion of which were ubiquitinated. The co-localization of TDP-43 with SGs could be fully prevented by inhibition of c-Jun N-terminal kinase (JNK). JNK inhibition did not prevent formation of HuR-positive SGs and did not prevent diffuse TDP-43 accumulation in the cytosol. In contrast, ERK or p38 inhibition prevented formation of both TDP-43 and HuR-positive SGs. JNK inhibition also inhibited TDP-43 SG localization in cells acutely treated with sodium arsenite and reduced the number of aggregates per cell in cultures transfected with C-terminal TDP-43 162-414 and 219-414 constructs.

Conclusions

Our studies are the first to demonstrate a critical role for kinase control of TDP-43 accumulation in SGs and may have important implications for development of treatments for FTD and ALS, targeting cell signal pathway control of TDP-43 aggregation.     

Activity profile of young soccer players during actual match play

This study investigated the activity profile of young soccer players (mean age 11.8 +/- 0.6 years; N = 12) with the aim of providing information for the development of training strategies. Data for movements of each player were obtained using 2 cameras that aimed at the subject throughout the match (Play Controller, Phromos, Italy). Encoders transmitted camera movements to a computer. The collected signals were then converted into distances, times, and speed attained at arbitrarily selected match categories. Players were monitored during official 11 vs. 11 matches (N = 12), with each match consisting of 2 halves, each lasting 30 minutes played on a regular soccer pitch. Mean total match distance amounted to 6,175 +/- 318 m. During the second half, players covered 5.5% less distance (p > 0.05). At speeds between 13.1 and 18.0 km.h(-1), players covered 12% less distance during the second half (p < 0.05). Players stood still 11% of the total time played (3,789 +/- 109 seconds). At speeds faster than 18 km.h(-1), players performed 33 +/- 4 bouts during match play, with a mean time length of 2.3 +/- 0.6 seconds per bout. Mean time interval between two successive maximal sprint bouts was 118.5 +/- 20.5 seconds. Players stood still longer during the course of the second half (229 +/- 76 seconds vs. 173 +/- 61 seconds, p < 0.05). Players tended to play in small areas of the football pitch and spent 9% of the total match time at high intensity. In order to promote a more active space coverage of the young player, at least for the age considered in the present study, the dimensions of the football pitch and the number of players should be reduced.     

Decomposition of sugarcane bagasse with lignocellulose-derived thermotolerant and thermoresistant Penicillia and Aspergilli

To promote the decomposition of sugarcane bagasse (SCB) for conversion into value-added products and to reduce waste, the capability of fungal mixes (FMs) to degrade SCB was examined. A total of 169 isolates from SCB and non-SCB were categorized as thermotolerant and thermoresistant. Thirty-six fungal candidates were screened for the presence of polyphenol oxidase, endoglucanase (EDN) and xylanase (XLN) activities, and EDN and XLN activities were quantitated. Five identified isolates (Aspergillus flavus AG10; Aspergillus niger AG68 & NB23; and Penicillium citrinum AG93 & AG140) were selected as the best enzyme producers, and 15 moderately to highly xylolytic, cellulolytic and ligninolytic isolates were added to construct FMs. Using a Taguchi design, the top ten reducing sugar-producing FMs (no. 12 showed the maximum amount of reducing sugar, at 2.11 mg g⁻¹, followed by no. 7, 15, 2, 16, 11, 13, 6, 4, & 8) were selected as potential agents for decomposition durations of 1, 2 and 3 months. The maximum decrease in SCB materials compared with the control was generated by FM 6 (9.08% cellulose reduction); FM 13 (21.03% hemicellulose reduction); and FM 16 (9.21% lignin reduction). These results indicate the potential use of SCB as a substrate for synergistic FMs. These FMs could be applied to the large-scale composting of SCB and other related agricultural residues, thus improving the biological pretreatment of lignocellulose.