Effect of soil moisture on bioremediation of chlorophenol-contaminated soil

A chlorophenol-contaminated soil was tested for the biodegradability in a semi-pilot scale microcosm using indigenous microorganisms. More than 90% of 4-chlorophenol and 2,4,6-trichlorophenol, initially at 30 mg kg^–1, were removed within 60 days and 30 mg pentachlorophenol kg^–1 was completely degraded within 140 days. The chlorophenols were degraded more effectively under aerobic condition than under anaerobic condition. Soil moisture had a significant effect with the slowest degradation rate of chlorophenols at 25% in the range of 10–40% moisture content. At 25–40%, the rate of chlorophenol degradation was directly related to the soil moisture content, whereas at 10–25%, it was inversely related. Limited oxygen availability through soil agglomeration at 25% moisture content might decrease the degradation rate of chlorophenols.     

植物、土壤及土壤管理对土壤微生物群落结构的影响

土壤微生物是土壤生态系统的重要组成部分,对土壤微生物群落结构多样性的研究是近年来土壤生态学研究的热点。本文综述了有关植物、土壤类型以及土壤管理措施对土壤微生物群落结构影响的最新研究结果,指出植物的作用因植物群落结构多样性、植物种类、同种植物不同的基因型,甚至同一植物不同根的区域而异;而土壤的作用与土壤质地和有机质含量等因素有关;植物和土壤类型在对土壤微生物群落结构影响上的作用存在互作关系。不同的土壤管理措施对土壤微生物群落结构影响较大,长期连作、大量的外援化学物质的应用降低了土壤微生物的多样性;而施用有机肥、免耕可以增加土壤微生物群落结构多样性,有利于维持土壤生态系统的功能。     

生物质炭对水稻土团聚体微生物多样性的影响

生物质炭施用对土壤微生物群落结构的影响已有报道,但土壤团聚体粒组中微生物群落对生物质炭施用的响应的研究还相对不足。以施用玉米秸秆生物质炭两年后的水稻土为对象,采用团聚体湿筛法,通过高通量测序对土壤团聚体的微生物群落结构与多样性进行分析,结果表明:(1)与对照相比,生物质炭施用显著促进了大团聚体(2000—250μm)的形成,并提高了团聚体的稳定性。(2)不同粒径团聚体间微生物相对丰度存在显著差异。在未施生物质炭的处理(C0)中,随着团聚体粒径增大,变形菌门、子囊菌门、β-变形杆菌目、格孢腔菌目的相对丰度逐渐降低,而酸杆菌门、担子菌门、粘球菌目、类球囊霉目的相对丰度逐渐升高。(3)生物质炭施用显著改变了团聚体间的微生物群落结构。与C0处理相比,生物质炭施用处理的大团聚体中变形菌门、鞭毛菌门和β-变形杆菌目的相对丰度分别显著提高了14.37%、33.28%和33.82%;微团聚体(250—53μm)中酸杆菌门、子囊菌门和粘球菌目的相对丰度分别显著降低了20.15%、19.93%和17.66%;粉、黏粒组分(<53μm)中担子菌门的相对丰度升高90.25%,而子囊菌门和鞭毛菌门的相对丰...     

耕作方式对潮土土壤团聚体微生物群落结构的影响

为探究不同耕作方式对潮土土壤团聚体微生物群落结构和多样性的影响,采用磷脂脂肪酸(PLFA)法测定了土壤团聚体中微生物群落。试验设置4个耕作处理,分别为旋耕+秸秆还田(RT)、深耕+秸秆还田(DP)、深松+秸秆还田(SS)和免耕+秸秆还田(NT)。结果表明:与RT相比,DP处理显著提高了原状土壤和>5 mm粒级土壤团聚体中真菌PLFAs量和真菌/细菌,为真菌的繁殖提供了有利条件,有助于土壤有机质的贮存,提高了土壤生态系统的缓冲能力;提高了5～2 mm粒级土壤团聚体中细菌PLFAs量,降低了土壤革兰氏阳性菌/革兰氏阴性菌,改善了土壤营养状况;提高了<0.25 mm粒级土壤团聚体中微生物丰富度指数。总的来说,深耕+秸秆还田(DP)对土壤团聚体细菌和真菌生物量有一定的提高作用,并且在一定程度上改善了土壤团聚体微生物群落结构,有利于增加土壤固碳能力和保持土壤微生物多样性。冗余分析结果表明,土壤团聚体总PLFAs量、细菌、革兰氏阴性菌和放线菌PLFAs量与土壤有机碳相关性较强,革兰氏阳性菌PLFAs量与总氮相关性较强。各处理较大粒级土壤团聚体微生物群落主要受碳氮比、含水量、pH值和团聚体质量分数的影响,较小粒级土壤团聚体微生物群落则主要受土壤有机碳和总氮的影响。     

水土保持林土壤抗蚀性能评价研究

运用土壤有机质含量、水稳性团聚体含量、水稳性团粒平均重量直径、团聚度和分散系数等各项指标,对不同树种组成、不同林龄水土保持林的土壤抗蚀性能进行分析、评价.结果表明,水土保持林对于提高土壤抗蚀性能具有重要作用,这种作用主要针对表层土壤而言;与油松纯林相比,油松阔叶树混交林土壤有机质含量较高,水稳性团聚体含量增加了1.71%～38.53%;且随着林龄的增长,水土保持林土壤抗蚀性能不断增强.     

基于土壤三相的广义土壤结构的定量化表达

以基于土壤固、液、气三相为研究对象的广义土壤结构为切入点,借鉴微观经济学中柯布-道格拉斯生产函数边际递减效益的思想,通过构建旱作土壤条件下土壤三相"投入"与土壤结构"产出"的土壤结构生产函数,定义了广义土壤结构指数(GSSI),GSSI=[(XS-A)XL XG]k.同时根据二维三系图中土壤结构相对理想三相点变化的趋势,计算了土壤三相结构距离(STPSD).结合典型黑土区马铃薯耕地土壤深松整地前后土壤结构的变化与广义土壤结构指数和土壤三相结构距离的计算结果,验证了广义土壤结构指数可以有效反映土壤结构特征及其动态变化.在此基础上,采用281个旱作土壤样本数据对广义土壤结构指数与土壤三相结构距离之间的关系进行了拟合,二者之间存在极显著的线性关系(R2 = 0.86* *).可以认为,广义土壤结构指数与土壤三相结构距离均能作为定量描述土壤结构变化的综合指标,不仅为深入研究广义土壤结构提供了一种新方法,而且为定量研究土壤结构、功能与质量奠定了基础.     

玉米秸秆还田培肥土壤的效果

辽北地区玉米根茬还田、秸秆直接还田或间接还田的3年微区培肥试验研究结果表明,无机肥的增产效果特别明显,而施有机物料,更主要的作用是改善土壤的物理、化学性质、培肥地力,与无肥对照相比,有机无机肥料配合施用可使土壤有机质提高3．06％－27．78％,各有机物料对土壤有机质提高的顺序依次为100％秸秆＞50％秸秆＞土粪＞牛粪＞33％秸秆＞根茬。在含C量相等的条件下,秸秆对土壤有机质的保持和提高好于土粪,土粪好于牛粪。同时,与单施化肥比,有机无机肥料配合施用可使土壤易氧化有机质增加10．91％－20．67％,使浸提腐殖酸提高1．43％－14．28％,使结合态腐殖酸的松／紧比值提高0．07－0．19,HA／FA比值提高0．07－0．24,并且能改善土壤的N、P、K营养状况、土壤水分和土壤孔隙状况,这标志着土壤有机质活性的提高和土壤肥力状况的改善。因此,应该大力提倡玉米秸秆秋季直接还田,其最佳施入量应为当年生产量的30％－50％。     

土壤侵蚀对土壤肥力及土地生物生产力的影响

通过对红壤坡地不同土地利用方式土壤肥力及土地生物生产力的空间分异研究,揭示了土壤侵蚀对土壤肥力和土地生产力的负面影响．即侵蚀导致Ｎ、Ｐ、Ｋ等土壤速效养分含量减少及其在坡面上部的相对贫乏和下部的相对富集;土壤有机质含量降低;土壤机械组成中砂、粉、粘粒比率发生变化,表现为土壤沙化,土地生物生产力下降．     

Effect of soil permeability on virus removal through soil columns

Laboratory experiments were performed on four different soils, using 100 cm long columns, to determine the extent of virus movement when wastewater percolated through the soils at various hydraulic flow rates. Unchlorinated secondary sewage effluent seeded with either poliovirus type 1 (strain LSc) or echovirus type 1 (isolate V239) was continuously applied to soil columns for 3 to 4 days at constant flow rates. Water samples were extracted daily from ceramic samplers at various depths of the column for the virus assay. The effectiveness of virus removal from wastewater varied greatly among the different soil types but appeared to be largely related to hydraulic flow rates. At a flow rate of 33 cm/day, Anthony sandy loam removed 99% of seeded poliovirus within the first 7 cm of the column. At flow rates of 300 cm/day and above, Rubicon sand gave the poorest removal of viruses; less than 90% of the seeded viruses were removed by passage of effluent through the entire length of the soil column. By linear regression analyses, the rate of virus removal in soil columns was found to be negatively correlated with the flow of the percolating sewage effluent. There was no significant difference in rate of removal between poliovirus and echovirus in soil columns 87 cm long. The rate of virus removal in the upper 17 cm of the soil column was found to be significantly greater than in the lower depths of the soil column. This study suggests that the flow rate of water through the soil may be the most important factor in predicting the potential of virus movement into the groundwater. Furthermore, the length of the soil column is critical in obtaining useful data to predict virus movement into groundwater.     

不同土壤采样设计下土壤表层微生物α多样性的差异分析

【背景】土壤采样是土壤研究的基础,采样方案的不同可能会对土壤微生物多样性的研究结果产生一定影响。【目的】研究不同的土壤采样设计方案对土壤样品16S rRNA基因高通量测序结果的影响。【方法】对2个不同生境样地的土壤进行网格化采样,对采集的18个土壤样品进行16S rRNA基因测序分析,通过模拟5种常见土壤采样方法,对比不同采样方式所获得的测序结果。【结果】不同采样方式会产生不同的测序结果。在测序深度有效的情况下,细菌总物种数随着采样数的增加而逐渐增长,增长速度在采样数大于5以后趋于平缓;样品中的优势物种(序列数200以上)只需很少的采样数(1-3)即可观察到全部物种;Shannon-Wiener指数与Simpson指数的变化较相似,当采样数由1到3时两指数均有较大增长,之后变化放缓。【结论】土壤细菌微生物测序研究中,土壤样地采样数量低于3个会影响测序结果的可靠性,采样方案选择梅花形采样法或蛇形采样法较为适宜。     

Effects of plant hedgerows on soil erosion and soil fertility on sloping farmland in the purple soil area

Effect of using plant hedgerows on controlling soil and water losses has received wide recognition and this technology has been applied in many areas in the world. Yet, studies on the effect of using plant hedgerows on soil fertility on sloping lands are rare. Carrying out an eight-year fixed field experiment, the authors investigated the effect of two different hedgerows against the control treatment on soil fertility. Results showed that clay particles tended to accumulate in front of the plant hedgerows and began to erode downward below the hedgerows along the contour lines across the field. Distribution of soil organic matter and all plant nutrients except potassium (K) showed the same pattern as the clay particles. Potassium, however, was evenly distributed in the field without any noticeable influence from the hedgerows. Since the fixed experiment started, soil phosphorus (P) kept accumulating, while soil organic matter and K were in depletion. The results accordingly suggested better nutrient management practices on the sloping lands by using properly reduced rates of P and increased rates of farm manure and K. Taking the sloping field as a whole, special attention in nutrient management should be given to the soil strips —the portions below the plant hedgerows suffering from more serious soil erosion.     

Effects of plant hedgerows on soil erosion and soil fertility on sloping farmland in the purple soil area

Effect of using plant hedgerows on controlling soil and water losses has received wide recognition and this technology has been applied in many areas in the world. Yet, studies on the effect of using plant hedgerows on soil fertility on sloping lands are rare. Carrying out an eight-year fixed field experiment, the authors investigated the effect of two different hedgerows against the control treatment on soil fertility. Results showed that clay particles tended to accumulate in front of the plant hedgerows and began to erode downward below the hedgerows along the contour lines across the field. Distribution of soil organic matter and all plant nutrients except potassium (K) showed the same pattern as the clay particles. Potassium, however, was evenly distributed in the field without any noticeable influence from the hedgerows. Since the fixed experiment started, soil phosphorus (P) kept accumulating, while soil organic matter and K were in depletion. The results accordingly suggested better nutrient management practices on the sloping lands by using properly reduced rates of P and increased rates of farm manure and K. Taking the sloping field as a whole, special attention in nutrient management should be given to the soil strips —the portions below the plant hedgerows suffering from more serious soil erosion.     

生物土壤结皮对荒漠土壤线虫群落的影响

在干旱的沙漠生态系统中,生物土壤结皮对于沙丘的固定和土壤生物的维持起着相当重要的作用.土壤线虫能敏感的指示土壤的恢复程度,是衡量沙区生态恢复与健康的重要生物学属性,而目前关于生物土壤结皮与土壤线虫的关系研究很少.为探明生物土壤结皮对土壤线虫的影响,以腾格里沙漠东南缘的人工植被固沙区藻结皮和藓类结皮覆盖的沙丘土壤为研究对象,根据固沙年限的不同将样地分为4个不同的区进行采样(1956、1964、1981和1991年固沙区),以流沙区作为对照;同时,在不同季度(4、7、9和12月)分别采集腾格里沙漠东南缘的人工植被固沙区藻结皮和藓类结皮覆盖下不同土层(0-10、10-20和20-30 cm)的沙丘土壤,以沙坡头地区的红卫天然植被区为对照,分析生物土壤结皮下土壤线虫的时空变化.采用改良的Baermann漏斗法进行分离线虫,用光学显微镜鉴定并统计.研究表明:1956、1964、1981和1991年人工植被固沙区的藻结皮和藓类结皮均可显著提高其下土壤线虫多度、属的丰富度、Shannon-Weaver多样性指数、富集指数和结构指数(P＜0.05),这可能是因为生物土壤结皮的存在为土壤线虫提供了重要的食物来源和适宜的生存环境;固沙年限与结皮下土壤线虫多度、属的丰富度、Shannon-Weaver多样性指数、富集指数和结构指数存在显著的正相关关系(P＜0.05),这说明固沙年限越久,越有利于土壤线虫的生存和繁衍;结皮类型显著影响土壤线虫群落,相对于藻结皮而言,藓类结皮下土壤线虫多度与属的丰富度更高(P＜0.05),这说明演替后期的藓类结皮比演替早期的藻结皮更有利于土壤线虫的生存和繁衍.此外,藻结皮和藓类结皮均可显著增加其下0-10、10-20和20-30 cm土层线虫多度和属的丰富度(P＜0.05),但随着土壤深度的增加,这种影响逐渐减弱,表明生物土壤结皮更有利于表层土壤线虫的生存;而且,随着季节的变化,藻结皮和藓类结皮下土壤线虫多度基本表现为秋季＞夏季＞春季＞冬季,这些反映了生物土壤结皮的生物量、盖度和种类组成随着季节变化而变化.因此,腾格里沙漠东南缘的人工植被固沙区生物土壤结皮的存在与演替有利于土壤线虫的生存和繁衍,增加了线虫数量、种类和多样性,这指示了生物土壤结皮有利于该区土壤及其相应生态系统的恢复.     

踩踏干扰对生物结皮土壤渗透性的影响

采用人工模拟降雨试验,研究了踩踏干扰对生物结皮土壤渗透性的影响.结果表明:踩踏干扰显著增加了土壤表面粗糙度,增加幅度与干扰强度有关,50%干扰度下表面粗糙度指数较不干扰增加91%.踩踏干扰延长了坡面产流时间,20%～50%干扰度范围内,随着干扰强度的增加,初始产流时间呈线性增加趋势,50%干扰度的初始产流时间较不干扰增加了169.7%.踩踏干扰增加了土壤渗透性,降低径流系数.50%干扰度的土壤累积入渗量较不干扰增加12.6%;去除生物结皮,土壤渗透性降低,累积入渗量较不干扰降低30.2%.50%以下的干扰度未显著增加土壤侵蚀模数.去除生物结皮,土壤侵蚀模数较不干扰增加10倍.生物结皮破碎度低于50%的干扰在不明显增加土壤流失量的前提下,可增加降水入渗,减小径流风险,改善土壤水分状况.     

施肥方式对紫色土土壤异养呼吸的影响

采用静态暗箱-气相色谱法于2010年12月至2011年10月对不同施肥方式下的紫色土土壤呼吸进行了研究,以揭示施肥方式对紫色土异养呼吸的影响。结果表明:施肥可对土壤异养呼吸产生激发效应。施肥后第5天出现峰值,猪厩肥处理的异养呼吸峰值为2356.8 mg CO2m-2h-1,显著高于秸秆配施氮磷钾(970.1 mgCO2m-2h-1)和常规氮磷钾处理(406.8 mgCO2m-2h-1)(P0.01);小麦季常规氮磷钾、猪厩肥和秸秆配施氮磷钾处理的平均土壤异养呼吸速率为212.9、285.8和305.8mgCO2m-2h-1,CO2排放量为255.1、342.3和369.5 gC/m2,玉米季为408.2、642.8和446.4 mgCO2m-2h-1,CO2排放量为344.7、542.8和376.9 gC/m2,玉米季土壤异养呼吸平均速率及CO2排放量均高于小麦季。全年平均土壤异养呼吸速率分别为310.6、446.3和377.4 mg CO2m-2h-1,CO2排放总量分别为599.8、885.1和746.4 gC/m2。猪厩肥对土壤异养呼吸速率和CO2排放量的影响最大,秸秆配施氮磷钾肥次之,氮磷钾肥最小,说明有机物料的投入是紫色土土壤异养呼吸速率的主要调控措施,低碳氮比的有机物料能促进土壤异养呼吸和CO2的排放。猪厩肥和秸秆配施氮磷钾肥处理相应地表和地下5 cm温度的Q10值分别为2.64、1.88和2.77、1.99,表明低碳氮比的有机物料还能增加土壤异养呼吸Q10值,使土壤异养呼吸速率对温度的敏感性加强。     

Effect of treatment of soil with keratinaceous material on soil fungi

In the early periods of soil storage, the total number of fungi almost regularly and significantly increased with the concentration of keratinaceous material (ground buffalo hooves) between 1% and 10%. A concentration of 20% was stimulative after 7 days and became toxic after 15 days and remained so through the end of the experiment. With increase in the storage period, the beneficial effect of all concentrations was lost and in the case of high concentrations (5-20%) toxic ammonia was produced. The occurrence of Scopulariopsis brevicaulis, Cephalosporium acremonium, Chrysosporium tropicum, C. indicum, and C. keratinophilum increased markedly, especially at the higher concentrations of the keratinaceous material and after long periods of incubation. With increase in the concentration of keratinaceous material, the rate of evolution of ammonia from the soil sharply increased during the second week after treatment and fell off sharply during the third and fourth weeks. In soil stored at 35 C, the rate of decline in total fungi after longer periods of incubation was sharper at 35 C than at 25 C. Also the amount of evolved ammonia during the first and the second weeks was almost always higher at 35 C than at 25 C. Fourteen species were tested for keratinolytic activity. Three of them were highly keratinolytic and five moderately so.     

植物篱对紫色土区坡耕地水土流失及土壤肥力的影响

植物篱的水土保持效果已得到广泛的认可,并在世界很多地方推广应用,然而,到目前为止,植物篱对坡耕地土壤肥力的影响规律研究却很少。利用长期定位小区试验,研究了植物篱对坡耕地土壤肥力的影响规律,旨在弄清植物篱提高土壤肥力的作用与效果,不断完善植物篱技术。研究发现,坡耕地在建立植物篱后,土壤粘粒在篱前富积,篱下加剧侵蚀,粘粒的富积与侵蚀沿等高线成水平带状分布;土壤有机质、N、P等主要营养元素出现与土壤颗粒相同的分布规律;对K来说,其分布不受植物篱的影响,表现出较为均一分布的特点。从土壤养分的绝对数量来看,P呈高度富积,而有机质和K则是高度耗竭。因此,坡耕地施肥时可以适当减少P的施用量,增加有机物和K的施用量。针对植物篱带对坡耕地肥力影响的特点,即篱前肥力升高,篱下肥力下降,在坡耕地管理上应特别加强篱下土壤带的培肥,以提高坡面整体生产能力。     

Distinct impacts of reductive soil disinfestation and chemical soil disinfestation on soil fungal communities and memberships

Applied Microbiology and Biotechnology - Soil disinfestation is an important agricultural practice to conquer soil-borne diseases and thereby ensure crop productivity. Reductive soil disinfestation...     

The influence of soil volume on the absorption of soil phosphorus by plants and on the determination of labile soil phosphorus

Summary In a pot experiment the soil volume available for ryegrass growth was varied. There was relatively greater uptake of phosphorus from the smaller amounts of soil and an increase in the pool of labile phosphorus (the L-value). It appears that an equilibrium exists in the soil between non-labile and labile phosphorus, and this equilibrium may be disturbed by the removal of phosphorus by plants.The variations of L-value with time followed a pattern indicating the initial influence of seed-borne phosphorus and slow isotopic exchange of the added carrier-free P³² with soil phosphorus.