Regional and Subcellular Distribution in Mammalian Brain of the Enzymes Producing Adenosine

The activities of 5'-nucleotidase, 2'-nucleotidase, alkaline phosphatase, and acid phosphatase were measured in rat and autopsied human brains. The four phosphatases in the rat brain showed little change in activity after death. The activities of adenosine-producing enzymes were compared in various parts of rat and human brains. When phosphatase activity was measured at pH 7.5, 5'-nucleotidase showed the highest activity in the most parts of the brain. The activity of 2'-nucleotidase and that of nonspecific phosphatase were almost the same at pH 7.5. However, higher phosphatase activity was observed in all parts of the brain when nonspecific phosphatase activity was measured at pH 10.0 or 5.5. High specific activity of 5'-nucleotidase in the brain was detected in the membranous components, especially in the synaptic membranes. The activity of 2'-nucleotidase was distributed in the soluble and synaptosomal fractions. The highest activity of both alkaline and acid phosphatases was recovered in the crude mitochondrial fraction, with the highest specific activity in the microsomal fraction. Phosphatase activity was distributed widely in the rat brain. The activity of 5'-nucleotidase was high in the medulla oblongata, thalamus, and hippocampus, but low in the peripheral nerve, spinal cord, and occipital lobe. The activity of 2'-nucleotidase was high in the vermis and frontal lobe. The highest acid and alkaline phosphatase activities were detected in the frontal lobe and in the olfactory bulb, respectively. The distribution of the four phosphatases in the autopsied human brain was similar to that in the rat brain. The highest 5'-nucleotidase activity was observed in the temporal lobe and thalamus.(ABSTRACT TRUNCATED AT 250 WORDS)     

羚牛(Budorcas taxicolor)部分脏器特点的观察

本文对2只羚牛的雌性生殖器官及肝、肾、脾等脏器进行了形态描述,并与黄牛及羊的相应器官进行了比较,为探讨羚牛的分类地位提供了解剖学资料。     

四川自贡大山铺蜀龙动物群——简报Ⅲ.蜥脚类

本文记述了中侏罗世蜥脚类一新属种——巴山酋龙(Datousaurus bashanensts gen. et sp. nov.)对李氏蜀龙(Shunosaurus lii)的特征进行了补充,讨论了它们在蜥脚类进化过程中的位置。     

Ocular Correlates of Inborn Metabolic Defects

The eye provides unique opportunities for the detection, during life, of deposits of storage substances and other characteristic changes resulting from inborn metabolic defects. The cornea shows the macromolecular polysaccharides of Hurler''s disease, the cystine crystals in cystinosis, and the copper deposits of Wilson''s disease. The sclera shows characteristic pigmentation in alcaptonuria. The iris shows the lack of pigmentation in various types of albinism. The lens is cataractous in galactosemia and dislocated in homocystinuria. The vitreous is opacified in familial amyloidosis. The retina shows different and characteristic deposits with the diseases of Tay-Sachs, Niemann-Pick, metachromatic leukodystrophy, and Farber''s lipogranulomatosis. The retinal veins show pronounced tortuosity with Fabry''s disease. There is some evidence that optic neuropathy occurs in glucose-6-phosphate dehydrogenase deficiency. Curiously, few abnormalities in the eye have been described in subjects with the glycogen storage diseases.     

记江西一新的中华弓鳍鱼化石

本文记述了在江西弋阳石溪组发现的一中华弓鳍鱼。其形态特征虽与华北的师氏中华弓鳍鱼很相似,但又有很明显的差别,因此建立一新种——Sinamia poyangica, sp. nov.。在此基础上,对中华弓鳍鱼的形态变异和石溪组的时代作了讨论,并对师氏中华弓鳍鱼的复原图提出了修正的建议。     

长吻鮠精巢及精子结构的研究

长吻鮠精巢高度分支呈指状。后1/3紫红色,由上皮细胞组成,既不产生精子,也不贮存精子。精巢的内部结构为叶型,由体细胞和生殖细胞构成,小叶的基本单位是小囊。精子头短而圆,主要为核占据,无顶体,核凹窝十分发达,有中心粒帽;尾极长,具侧鳍,轴丝基部有发达的囊泡状结构和线粒体。     

Karyotype Analysis of 5 Species of Polygonatum Mill.

The present paper reports the chromosome numbers and karyotypes of five species in Polygonatum from Anhui of China. The materials used in this work are listed in Table 1, Photomicrographs of somatic metaphase and karyograms of the five species of Polygonatum in Plate 1, 2, 3, the idiograms in Fig. 1-11 and a comparison of the karyotype of them is provided in Table 2. The results are shown as follows: 1. Polygonatum odoratum (Mill.)Druce Two materials were examined. One from Mt. Huangshan, Anhui, has 2n= 16 = 10m (3sc)+ 6sm (Plate 1 :A, B). The idiogram is shown in Fig. 1. The chromosomes range in length from 2.85 to 8.85 μm, with the total length 48.63μm and the ratio of the longest to the shortest 3.11, The karyotype belong to Stebbins’(1971) 2B. The two chromosomes of the first pair have arm ratios 1.01 and 1.29 respectively, and The first pair has one chromosome carrying a satellite attached to the short arm, showing heterozyosity .The chromosome num ber of 2n= 16 in P. odoratum and its karyotype are reported for the first time. The other from Langyashan, Chu - xian, Anhui, is found to have 2n = 18 = 10m (Isc)+2sm+6st(2sc) (Plate 1: C, D). The idiogram is shown in Fig. 2. The chromosomes range in length from 2.43 to 8.29μm, with the total length 46.67µm and the ratio of the longest to the shortest 3.41. The karyotype is also of 2B. In a somatic chromosome complement the 2nd pair have one chromosome carrying a satellite attached to the long arm, showing heterozygosity. 2. Polygonatum filipes Merr. Two materials were examined. One from the Huangshan, Anhui is found to have two cytotypes: 2n= 16 and 2n=22. This paper reports one of them. The karyotype formula is 2n=22=8m+8sm(2sc)+6st(Plate 3: Q, R). The idiogram is shown in Fig. 3. The chromosomes range in length from 2.55- 5.85μm, with the total length 45.01 μm and the ratio of the longest to the shortest 2.29. The karyotype belongs to 3B. The other material from the Fangchang, Anhui, is shown to have four cytitypes: 2n= 14, 2n= 16, 2n=20 (Plate 3: W) and 2n=22. This paper reports two of them. Type I: the karytype formula is 2n=14=10m+4sm (Plate 3: S, T). The idiogram is shown in Fig. 5. The chromosomes range in length from 2.59 to 7.61μm, the total length 37.44μm and the ratio of the longest to the shortest is 2.94. the karyotype belongs to 2B. Type II :The karyotype formula is 2n=16=8m+4sm+4st (Plate 3: U, V). The idiogram is shown in Fig. 4. The chromosomes range in length from 2.65 to 8.21 μm, the total length 46.01 μm and the ratio of the longest to the shortest 3.10. The karyotype belongs to 2B. The chromosome numbers of 2n=20, 2n= 14 and 2n=22, and karyotype of 2n= 14 and 2n=22 in P. filipes are reported for the first time. 3. Polygonatum cytonema Hua Two materials were examined. One from the Langyashan, Chuxian, anhui, is found to have 2n = 18 = 8m (2sc)+ 6sm+ 4st (Plate 2: K, L). The idiogram is shown in Fig. 7. The chromosomes range in length from 3.41 to 9.21 μm, the total length 56.34μm and the ratio of the longest to the shortest is 2.70. The karyotype belongs to 2B. The other material from the Huangshan, Anhui, has two cytotypes: 2n=20 and 2n= 22. Type I: The karyotype formula is 2n= 20= 8m+ 6sm+ 6st (Plate 2: M, N). The idiogram is shown in Fig. 8. The chromosomes range in length from 1.75 to 5.03μm, with the total length 32. 91μm and the ratio of the longest to the shortest 2. 87. The karyotype is also of 2B. Type II: The karyotype formula is 2n=22=6m+ 8sm+4st+ 4t (Plate 2: O, P ). The idiogram is Shown in Fig. 10. The chromosomes range in length from 1.75 to 4.95 μm, with total length 35.05μm and the ratio of the longest to the shortest 2.83. The karyotype brlongs to 3B. 4. Polygonatum desoulayi kom. The material from Xuancheng, Anhui, is found to have karyotype 2n = 22 = 10m (2sc) + 6sm (lsc) + 6st ( Plate 2. I, J). The idiogram is shown in Fig. 6. The chromosomes range in length from 1.86 to 5.61μm, with the total length 41.98μm and the ratio of the longest to the shortest 3.02. The karyotype is also of 3B. The first pair has one chromosome carrying a satellite attached to the long arm, showing heterozygosity. The chromosome number and karyotype of Chinese material are reported for the first time. 5. Polygonatum verticillatum (L.) All. The material from the Langyashan, Chuxian, Anhui is found to have two cytotypes. Type 1: the karyotype formula is 2n = 18 = 2m+ 2sm+ 10st+ 2t+ 2T (Plate 1: G, H). The idiogram is shown in Fig.9. The chromosomes range in length from 1.86 to 4.03μm, with total length 28.28μm and the ratio of the longest to the shortest 2.17. The karyotype classification belongs to 3B. Type II: The karyotype formula is 2n=24=6m+4sm+12st+2T (Plate 1: E, F). The idiogram is shown in Fig. II. The chromosomes range in length from 2.01 to 5.03μm, with total length 41.36μm and the ratio of longest to shortest 2.50. The karyotype is also of 3B. The chromosome numbers and karyotypes of Chinese material are reported for the first time.     

西安市青年学生胸骨长与身长的关系

本文对1980年测量的西安在校汉族青年学生1585名(男863,女722),年龄16-24岁,按年龄性别分组,计算了身长和胸骨长的均值、胸骨长占身长的百分数、身长与胸骨长的比值、身长、胸骨长指数,并提出了由胸骨长推算身长的回归方程。     

The Phloem of Nelumbo nucifera Gaertn

In common with other aquatic angiosperms, Nelumbo nucifera Gaertn.has a relatively strongly developed phloem tissue. The vascularsystem consists of discrete collateral bundles in which no cambiumdevelops and the phloem and xylem are separated by a narrowlayer of parenchyma cells. The phloem consists of sieve elements,companion cells, and phloem parenchyma cells. The sieve elementshave transverse end walls with simple sieve plates. The cellsattain considerable width in the late phloem (metaphloem). Thecompanion cells are in vertical strands. In the early phloem(protophloem) of large bundles the sieve tubes and companioncells are eventually obliterated. The parenchyma cells alsoform vertical strands which may contain tannin cells. Some parenchymacells and companion cells are binucleate. The sieve elementsshow ultrastructural features common for these cells in dicotyledons.At maturity, they lack nuclei, ribosomes, and tonoplasts, butretain a plasmalemma, mitochondria, and plastids. The latterare poorly differentiated and form starch. The endoplasmic reticulumis in part stacked, in part it forms a network next to the plasmalemma.The P-protein occurs in two forms. One consists of tubules notassembled in any specific type of array. The other, possiblycomposed of much extended tubules, is assembled in crystallineaggregates which are retained as such in mature cells. The sieveplate pores are lined with callose and plasmalemma. The lateralwalls are relatively thin and the nacreous layer varies in degreeof distinctness.     

巨龙竹生殖器官形态结构及雌、雄配子体的发育

通过石蜡切片的方法对巨龙竹生殖器官结构、大小孢子的发生和雌、雄配子体的发育过程进行了观察研究。 巨龙竹为一心皮组成的单室单子房,子房内具有一个胚珠,倒生、双珠被、厚珠心。大孢子母细胞减数分裂形成线形排列的4个大孢子,合点端大孢子具功能。胚囊的发育为蓼型,具多个反足细胞。巨龙竹的花药壁由4层结构组成,包括表皮、药室内壁、中层、绒毡层;花药壁发育为单子叶型,绒毡层为腺质型。小孢子母细胞减数分裂中的胞质分裂为连续型,四分孢子为四面体型;成熟花粉粒为2细胞型,具1个萌发孔。小穗发育雌雄异熟,雌蕊的发育早于雄蕊的发育。     

陕西秦岭半仓山蝎蛉的生物学观察

2004~2005年,在陕西省南部米仓山黎坪国家森林公园研究了秦岭蝎蛉Panorpa qinlingensisChouetRan的年生活史和生物学习性,通过饲养,成功获得卵、幼虫、蛹等虫态。结果表明,秦岭蝎蛉在米仓山1年发生2代,以预蛹在土室里越冬,5月中旬始见越冬代成虫,5月中旬末~6月上旬为越冬代成虫羽化盛期,7月下旬~8月中旬为第1代成虫羽化盛期,成虫在室内可存活35~60 d。羽化近1周后开始交尾,交尾4~8 d后开始产卵,单雌产卵量35~180粒,卵期5~9 d;幼虫型,共4龄,历期38~50d;蛹为强颚离蛹,蛹期8~18 d。记述了主要生物学习性。     

The ultrastructure of corpus allatum of the earwig Euborellia annulipes lucas (Dermaptera: Labiduridae)

The ultrastructure of corpus allatum of the earwig, Euborellia annulipes has been described. The corpus allatum (CA) is an oval body. The gland is covered by a thin stromal sheath which is wavey and acellular in composition. The gland consists of either parenchymal cells with distinct, double-layered, smooth or slightly undulated plasma-membranes. Golgi bodies and endoplasmic reticulum are poorly developed. The mitochondria are found in abundance and are oval, spherical, elongate, and Y-shaped, with oblique and longitudinal cristae. The gland is innervated by neurosecretory and non-neurosecretory axons. The neurosecretory axons are of two types. The type NS-II, contain electron dense granules of 40...120 nm in diameter and the type NS-I, small less electron dense granules (of mixed nature) 40...90 nm in diameter. Branches of tracheoles also occur that penetrate the gland. The large electron dense granules 100...220 nm in diameter occuring abundantly in aorta, and representing the secretions of the medial A-cells are absent in the CA.     

鲁南地区花生昆虫群落结构及多样性变化研究

采用马来氏网法在山东省临沂市开展了花生田昆虫系统调查(2014年6月-2015年6月),阐明当地花生田的昆虫群落结构及相关群体指数动态规律。结果表明:试验期内共获得昆虫样本65 005头,分属于14目、129科。从目级水平来看,主要花生昆虫以膜翅目、鞘翅目、半翅目、双翅目和弹尾目为主;从科级水平来看,主要花生害虫有叶蝉科、夜蛾科和潜蝇科,主要天敌有花蝇科、长足虻科、金小蜂科、长蝽科和姬蜂科。该地区群落多样性指数在0.4262～3.3212之间,峰值出现在花生生长期(3.3312);相对丰度峰值出现在花生繁育期(9 956);科级丰富度峰值出现也出现在花生繁育期(83);均匀度指数在0.1137～0.7613之间,峰值出现在2014年6月(0.7613);优势集中指数在0.0172～0.2134之间,峰值出现在2014年8月(0.2134)。     

崇左金花茶花朵和叶片类黄酮UPLC-Q-TOF-MS分析

以崇左金花茶（Camellia chuangtsoensis）为材料,利用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS）联用技术定性定量分析其花朵（花瓣、雄蕊）和叶片（老叶、新叶）中类黄酮成分与含量。结果表明,崇左金花茶中共检测到14种类黄酮成分,木犀草素、木犀草素-7-O-芸香糖苷、槲皮素-3,7-O-二葡萄糖苷、芸香柚皮苷、圣草素和染料木苷为山茶属金花茶组植物中首次发现,其中槲皮素-3,7-O-二葡萄糖苷、芸香柚皮苷、圣草素和染料木苷主要存在于花朵中,木犀草素和木犀草素-7-O-芸香糖苷在花朵中含量高于叶片,雄蕊中高于花瓣;槲皮素-3-O-葡萄糖苷、槲皮素-7-O-葡萄糖苷、槲皮素-3-O-芸香糖苷和山柰酚-3-O-葡萄糖苷为金花茶组植物叶片中首次发现,其叶片中含量远低于花朵,老叶中远低于新叶,雄蕊中远低于花瓣;儿茶素和表儿茶素在花朵中含量高于叶片,雄蕊中高于花瓣;槲皮素和山萘酚在花朵和叶片中含量均较低。崇左金花茶花瓣和雄蕊中含量较高的类黄酮为儿茶素类、木犀草素类和槲皮素类,主要是表儿茶素、木犀草素和槲皮素-3-O-葡萄糖苷;叶片中为儿茶素类和木犀草素类,主要是表儿茶素、木犀草素和木犀草素-7-O-芸香糖苷。崇左金花茶花瓣和雄蕊中儿茶素类、木犀草素类及类黄酮总量均高于叶片,且雄蕊高于花瓣;花瓣和雄蕊中槲皮素类远高于叶片,且花瓣中远高于雄蕊。     

四川凉山彝族传统民俗中的植物及其文化意义

凉山彝族传统民俗植物文化在当地彝社会中广泛存在,表现了丰富多彩的文化多样性,集中反映在法术仪式、礼仪、节日庆典等方面。采用关键人物访谈、半结构访谈、参与式观察等方法研究了凉山彝族传统民俗中使用的植物,共调查到102种,其中,跟婚礼相关的植物21种,葬礼植物24种,成人礼植物11种,日常礼仪植物3种;火把节植物14种,彝族年植物15种;法术仪式植物91种。这些民俗植物的文化含义主要表现在驱鬼、生殖崇拜和祖先崇拜三个方面,这与当地彝族崇信鬼神和其崇拜万物有灵的原始宗教有关,是其自然原始宗教和毕摩文化的重要组成。     

平疣桑椹石磺精子结构观察

通过电光学显微镜和透射电子显微镜观察了平疣桑椹石磺精子的形态及其超微结构。平疣桑椹石磺成熟精子属于进化型,由头部、中段和末段组成。头部由顶体和精核构成,顶体长约0.7μm,呈细奶嘴状,内含物分布均匀,电子密度稍低于细胞核。顶体基部与精核前端紧密相连,无间隙。精核长约3.8μm,宽约1.0μm,核质高度浓缩,电子密度高,无核泡,纵切似辣椒状,核后端内凹形成核后窝。中段加长,结构复杂,线粒体演化成线粒体鞘,螺旋状包绕轴丝。精子末段由轴丝及包绕轴丝的质膜组成,轴丝为典型的“9＋2”结构。比较了平疣桑椹石磺精子与相关腹足类精子结构的异同,进一步证实了腹足纲贝类精子结构之间的区别主要在于顶体有无及形态,精核的长短与外形、中段线粒体的数目及其排列方式等。     

27种木犀属植物叶表皮微形态特征的研究

利用光学显微镜和扫描电子显微镜观察了27种木犀属植物的叶表皮,测量并统计了气孔器类型、气孔大小、气孔密度、气孔指数及腺点密度等指标,以明确各种的叶表皮细胞形状及垂周壁式样、表皮角质膜、蜡质纹饰及气孔外拱盖的具体特征。结果显示:木犀属植物叶下表皮有气孔器,形状为圆形、椭圆形;气孔器类型多为不规则型,只有总状桂花和狭叶木犀为环列型;气孔器外围角质层有放射状、条状、环状、颗粒状等多种类型;叶表皮细胞形状有无规则形和多边形2种;下表皮腺点的数目远远大于上表皮。研究表明,木犀属植物气孔器和叶表皮细胞特征在种之间差异比较明显,可以作为种间鉴定的重要依据,具有重要的分类学意义。     

The relationship of γ-aminobutyrate levels and its metabolism to age in brains of mice

The activities of glutamate decarboxylase, aspartate aminotransferase, and γ-aminobutyrate aminotransferase were determined in the brains of C57B1/6J mice of selected biological ages (10, 24, 33, and 37 months old). The glutamate decarboxylase activity was the same in the 10 and 24 month mice, but was decreased in the 30 and 37 month mice. The aspartate aminotransferase activity was constant in the 10, 24, and 33 month old mice and was slightly decreased in the 37 month mice. The γ-aminobutyrate aminotransferase activity was the same in the brains of the 10 and 24 month old mice and was increased in the 33 month mice and even more in the 37 month mice.The steady state levels of aspartate, glutamate, and γ-aminobutyrate were determined in the brains of mice 10, 18, 29, and 36 months old. The concentration of aspartate was the same in the 10, 18, and 29 month mice and was increased in the 36 month mice. The mice in all the age groups had the same brain concentration of glutamate. γ-Aminobutyrate concentration decreased somewhat with age.The weights of the brains did not vary with age from 10 to 33 months. The wet weights of the brains of the 36 and 37 month mice were lower than those of all the other age groups. The amount of protein per gram of wet weight of brain did not vary with age.     

Serum immunoglobulin levels in various forms of meningococcal infection and in meningococcus carriers

The levels of serum IgG, IgA and IgM were examined in 191 adults including 103 patients with various forms of meningococcal infection, 32 meningitis convalescents and 56 carriers, in order to elucidate the causes of different susceptibility to the meningococcal infection. The IgD level was determined in 54 meningitis patients as well as in convalescents and carriers. The amount of immunoglobulins was determined by radial immunodiffusion. The level of IgG at the beginning of the disease in patients with the generalized forms of meningococcal infection (meningitis, meningitis combined with meningococcaemia, meningococcaemia) was found to be considerably lower than in healthy subjects. The levels of all immunoglobulins, particularly of IgA and IgM, increased in the course of the disease. The levels of IgG, IgA and IgM in meningitis convalescents a year after recovery were found to be the same as in the controls. The levels of IgG, IgA and IgM in patients with meningococcal nasopharyngitis were significantly lower than in healthy subjects. The carriers showed a decreasd level of IgA and a considerably increased level of IgG while the levels of IgM and IgD did not differ from the control.     

大叶杨配囊及胚珠的形成和发育

本文应用细胞化学方法研究了大叶杨胚珠、胚囊的形成和发育过程中核酸、蛋白质及不溶性多糖的分布和消长。大孢子母细胞、大孢子四分体及功能大孢子中含较少不溶性多糖,但却含丰富的RNA和蛋白质。功能大孢子经分裂发育成八核的蓼型胚囊。四核胚囊开始积累细胞质多糖,成熟胚囊中除反足细胞外充满淀粉粒。反足细胞形成后不久即退化。助细胞具多糖性质的丝状器,受精前两个助细胞退化。卵细胞核对Feulgen反应呈负反应。二极核受精前由胚囊中部移向卵器,与卵器接触后融合形成次生核。发育早期的胚珠为厚珠心,双珠被。晚期,内珠被退化,故成熟胚珠为单珠被。四核胚囊时期,珠孔端珠心组织退化,胚囊伸向珠孔形成胚囊喙。合点端珠心组织含丰富的蛋白质和核酸,这一性质与绒毡层性质相似,可能涉及胚囊的营养运输。胚囊的营养来源于子房和胎座细胞内贮存的淀粉粒。