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1.
水稻白叶枯病和水稻细菌性条斑病是由稻黄单胞细菌(Xanthomonas oryzae)不同致病变种引起的两种最重要的水稻细菌性病害。发掘和利用抗性基因,培育抗病品种是防治这两种病害的最有效手段之一。本文分别综述了这两种高度相关的病害的抗性遗传研究进展,包括已发掘和利用的主效抗性基因特点及目前国内外对这两种病害的抗性QTL定位研究进展,为水稻抗白叶枯病和细菌性条斑病育种研究提供有用信息。 相似文献
2.
Molecular markers linked to stem rot resistance in rice 总被引:4,自引:0,他引:4
J. Ni P. M. Colowit J. J. Oster K. Xu D. J. Mackill 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):511-516
Stem rot (Sclerotium oryzae) is an important disease constraint in Californian rice production. Measurement of resistance is laborious, and the low heritability
of the trait limits the effectiveness of selection in breeding programs. Molecular markers linked to the trait would therefore
provide a superior selection screen to assist in transferring resistance into improved cultivars. The genetics of resistance
to stem rot was studied in the germplasm line 87-Y-550 (PI566666), which inherited its resistance from the wild species Oryza rufipogon. Four crosses of 87-Y-550 with susceptible lines were made and recombinant inbred lines of only the most-resistant and most-susceptible
progeny within each cross were advanced for late-generation testing. Approximately 900 AFLP (amplified fragment length polymorphism)
primer combinations were applied to resistant and susceptible bulks within each cross. One AFLP marker showed significant
association with stem rot resistance and accounted for approximately 45.0% of the phenotypic variation in 59 progenies. This
marker was mapped on rice chromosome 2 between the RFLP markers RZ166 and RG139 by using F2-reference population information. The accuracy of AFLP marker mapping was validated by size and sequence comparison of AFLP
bands from 87-Y-550 and the reference population. With the strategy of selective genotyping combined with a parental survey,
two microsatellite markers, RM232 and RM251, on chromosome 3 were also found associated with stem rot resistance and accounted
for 41.1% and 37.9% of the phenotypic variation, respectively. The multiple linear regression model included TAA/GTA167 on
chromosome 2 and RM232 on chromosome 3 and cumulatively explained 49.3% of total variation. The molecular markers linked to
stem rot resistance should facilitate selection for this recalcitrant trait in rice breeding programs by eliminating the need
for early generation screening.
Received: 27 March 2000 / Accepted: 4 June 2000 相似文献
3.
Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR 总被引:56,自引:0,他引:56
N. Huang E. R. Angeles J. Domingo G. Magpantay S. Singh G. Zhang N. Kumaravadivel J. Bennett G. S. Khush 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(3):313-320
DNA marker-assisted selection was used to pyramid four bacterial blight resistance genes, Xa-4, xa-5, xa-13 and Xa-21. Breeding lines with two, three and four resistance genes were developed and tested for resistance to the bacterial blight
pathogen (Xanthomonas oryzae pv. oryzae). The pyramid lines showed a wider spectrum and a higher level of resistance than lines with only a single gene. To speed
up the gene pyramiding process and to facilitate future marker-aided selection, we developed PCR markers for the two recessive
genes, xa-5 and xa-13, and used these to survey a range of rice germplasm. The results of the germplasm survey will be useful for the selection
of parents in breeding programs aimed at transferring these bacterial blight resistance genes from one varietal background
to another.
Received: 6 December 1996/Accepted: 20 December 1996 相似文献
4.
Construction of a BAC contig containing the xa5 locus in rice 总被引:9,自引:0,他引:9
D. Yang A. Sanchez G. S. Khush Y. Zhu N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1120-1124
The recessive gene xa5 confers resistance to bacterial blight in rice. To generate a physical map of the xa5 locus, three RFLP markers RG556, RG207 and RZ390, closely linked to xa5, were used to screen a rice bacterial artificial chromosome (BAC) library. The identified overlapping BAC clones formed two
small contigs which were extended to both sides by chromosome walking. The final physical map consisted of 14 BAC clones and
covered 550 kb. Genetic analysis with an F2 population showed that two RFLP markers 28N22R and 40F20R, derived from the BAC clones in the contig, flanked the xa5 locus. To further delimit the location of the xa5 locus, RFLP markers RG556 and RG207 were converted to sequence tagged sites and used to perform genetic analysis. The results
indicated that the xa5 locus was most likely located between RG207 and RG556. Among the BAC clones in the contig, one clone, 44B4, hybridized to
both RG207 and RG556. This suggests that BAC clone 44B4 carried the xa5 locus.
Received: 12 January 1998 / Accepted: 27 May 1998 相似文献
5.
Mapping of QTLs conferring resistance to bacterial leaf streak in rice 总被引:13,自引:0,他引:13
D. Tang W. Wu W. Li H. Lu A. J. Worland 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):286-291
A large F2 and a RI population were separately derived from a cross between two indica rice varieties, one of which was highly resistant to bacterial leaf streak (BLS) and the other highly susceptible. Following
artificial inoculation of the RI population and over 2 years of testing, 11 QTLs were mapped by composite interval mapping
(CIM) on six chromosomes. Six of the QTLs were detected in both seasons. Eight of the QTLs were significant following stepwise
regression analysis, and of these, 5 with the largest effects were significant in both seasons. The detected QTLs explained
84.6% of the genetic variation in 1997. Bulked segregant analysis (BSA) of the extremes of the F2 population identified 3 QTLs of large effect. The 3 QTLs were dentical to 3 of the 5 largest QTLs detected by CIM. The independent
detection of the same QTLs using two methods of analysis in separate mapping populations verifies the existence of the QTLs
for BLS and provides markers to ease their introduction into elite varieties.
Received: 13 October 1999 / Accepted: 29 October 1999 相似文献
6.
QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice 总被引:14,自引:0,他引:14
S. Fukuoka K. Okuno 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):185-190
Field resistance is defined as the resistance that allows effective control of a parasite under natural field conditions and
is durable when exposed to new races of that parasite. To identify the genes for field resistance to rice blast, quantitative
trait loci (QTLs) conferring field resistance to rice blast in Japanese upland rice were detected and mapped using RFLP and
SSR markers. QTL analysis was carried out in F4 progeny lines from the cross between Nipponbare (moderately susceptible, lowland) and Owarihatamochi (resistant, upland).
Two QTLs were detected on chromosome 4 and one QTL was detected on each of chromosomes 9 and 12. The phenotypic variation
explained by each QTL ranged from 7.9 to 45.7% and the four QTLs explained 66.3% of the total phenotypic variation. Backcrossed
progeny lines were developed to transfer the QTL with largest effect using the susceptible cultivar Aichiasahi as a recurrent
parent. Among 82 F3 lines derived from the backcross, resistance segregated in the expected ratio of resistant 1 : heterozygous 2 : susceptible
1. The average score for blast resistance measured in the field was 4.2 ± 0.67, 7.5 ± 0.51and 8.2 ± 0.66, for resistant, heterozygous
and susceptible groups, respectively. The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively. The relationship to previously reported major genes and QTLs conferring
resistance to blasts, and the significance of marker-assisted selection to improve field resistance, are discussed.
Received: 8 June 2000 / Accepted: 24 November 2000 相似文献
7.
Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106 总被引:10,自引:0,他引:10
S. Singh J. S. Sidhu N. Huang Y. Vikal Z. Li D. S. Brar H. S. Dhaliwal G. S. Khush 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(6-7):1011-1015
Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major disease of rice in several countries. Three BB resistance genes, xa5, xa13 and Xa21, were pyramided into cv. PR106, which is widely grown in Punjab, India, using marker-assisted selection. Lines of PR106 with
pyramided genes were evaluated after inoculation with 17 isolates of the pathogen from the Punjab and six races of Xoo from the Philippines. Genes in combinations were found to provide high levels of resistance to the predominant Xoo isolates from the Punjab and six races from the Philippines. Lines of PR106 with two and three BB resistance genes were also
evaluated under natural conditions at 31 sites in commercial fields. The combination of genes provided a wider spectrum of
resistance to the pathogen population prevalent in the region; Xa21 was the most effective, followed by xa5. Resistance gene xa13 was the least effective against Xoo. Only 1 of the BB isolates, PX04, was virulent on the line carrying Xa21 but avirulent on the lines having xa5 and xa13 genes in combination with Xa21.
Received: 26 May 2000 / Accepted: 16 August 2000 相似文献
8.
S. Garland L. Lewin A. Blakeney R. Reinke R. Henry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(3):364-371
The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that
was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based
marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped
near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles
from non-fragrance-producing alleles at the fgr locus.
Received: 19 October 1999 / Accepted: 16 December 1999 相似文献
9.
Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers 总被引:4,自引:0,他引:4
Y. Hayano-Saito T. Tsuji K. Fujii K. Saito M. Iwasaki A. Saito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1044-1049
We used graphical genotyping and linkage analyses with molecular markers to determine the chromosomal location of the rice
stripe disease resistance gene, Stv-b
i
. The stripe resistance gene from the indica rice (Oryza sativa) cv ‘Modan’ was introgressed into several Japanese rice varieties. We found 4 RFLP markers in ‘Modan’, five susceptible parental
rice varieties (‘Norin No. 8’, ‘Sachihikari’, ‘Kanto No. 98’, ‘Hokuriku No.103’ and ‘Koganebare’) and four resistant progeny
varieties (‘St. No. 1’, ‘Aichi No. 6’, ‘Aoisora’ and ‘Asanohikari’). Graphical genotyping of the resistant progeny revealed
a chromosomal segment ascribable to ‘Modan’ and associated with stripe resistance. The chromosomal segment from ‘Modan’ was
located at 35.85 cM on chromosome 11. Linkage analysis using 120 F2 individuals from a cross between ‘Koshihikari’ (susceptible) and ‘Asanohikari’ (resistant) revealed another 8 RFLP markers
in the same chromosome. We performed a bioassay for rice stripe resistance in F3 lines of the F2 individuals using infective small brown planthoppers and identified an 1.8-cM segment harboring the rice stripe disease resistance
gene, Stv-b
i
, between XNpb220 and XNpb257/ XNpb254. Furthermore, Stv-b
i
was linked by 0.0 cM to a RFLP marker, ST10, which was developed on the basis of the results of RAPD analysis. These DNA
markers near the Stv-b
i
locus may be useful in marker-assisted selection and map-based cloning of the Stv-b
i
gene.
Received: 26 September 1997 / Accepted: 4 November 1997 相似文献
10.
Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar 总被引:12,自引:0,他引:12
D.-H. Chen M. dela Viña T. Inukai D. J. Mackill P. C. Ronald R. J. Nelson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1046-1053
A recombinant inbred line derived from a cross between CO39 and ‘Moroberekan’, RIL276, was found to be resistant to lineage
44 isolates of Pyricularia grisea in the Philippines. One hundred F2 individuals were obtained from a backcross of RIL276 and CO39. Phenotypic analysis showed that RIL276 carries a single locus,
tentatively named Pi44(t), conferring complete resistance to lineage 44 isolates of P. grisea. RFLP probes, STS primers and AFLP markers were applied to identify DNA markers linked to Pi44(t). Neither RFLP nor STS-PCR analysis gave rise to DNA markers linked to the locus. Using bulk segregant AFLP analysis, however,
two dominant AFLP markers (AF348 and AF349) linked to Pi44(t) were identified. AF349 and AF348 were located at 3.3±1.5 cM and 11±3.5 cM from Pi44(t), respectively. These markers were mapped on chromosome 11 using an F2 population derived from a cross between ‘Labelle’ and ‘Black Gora’. The location of AF348 on chromosome 11 was confirmed using another F2 mapping population derived from IR40931-26-3-3-5/ PI543851. DNA products at the loci linked to Pi44(t) were amplified from RIL276, ‘Labelle’ and PI543851 using the same primer pairs used to amplify AF349 and AF348. Sequence analysis of these bands showed 100% identity between lines. This result indicates that these AFLP markers could
be used for the comparison of maps or assignment of linkage groups to chromosomes.
Received: 12 May 1998 / Accepted: 13 November 1998 相似文献
11.
Genetic and physical mapping of xa13, a recessive bacterial blight resistance gene in rice 总被引:6,自引:0,他引:6
A. C. Sanchez L. L. Ilag D. Yang D. S. Brar F. Ausubel G. S. Khush M. Yano T. Sasaki Z. Li N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1022-1028
The recessive gene, xa13, confers resistance to Philippine race 6 (PXO99) of the bacterial blight pathogen Xanthomonas oryzae pv oryzae. Fine genetic mapping and physical mapping were conducted as initial steps in an effort to isolate the gene. Using nine selected
DNA markers and two F2 populations of 132 and 230 plants, xa13 was fine-mapped to a genomic region <4 cM on the long arm of rice chromosome 8, flanked by two RFLP markers, RG136 and R2027.
Four DNA markers, RG136, R2027, S14003, and G1149, in the target region were used to identify bacterial artificial chromosome
(BAC) clones potentially harboring the xa13 locus from a rice BAC library. A total of 11 BACs were identified, forming four separate contigs including a single-clone
contig, 29I3, associated with the RG136 STS marker, the S14003 contig consisting of four clones (44F8, 41O2, 12A16, and 12F20),
the G1149 contig with two clones, 23D11 and 21H18, and the R2027 contig consisting of four overlapping clones, 42C23, 30B5,
6B7 and 21H14. Genetic mapping indicated that the xa13 locus was contained in the R2027 contig. Chromosomal walking on the R2027 contig resulted in two more clones, 33C7 and 14L3.
DNA fingerprinting showed that the six clones of the R2027 contig were overlapping. Clone 44F8 hybridized with a single fragment
from the clone 14L3, integrating the R2027 and S14003 contigs into a single contig consisting of ten BAC clones with a total
size of approximately 330 kb. The physical presence of the xa13 locus in the contig was determined by mapping the ends of the BAC inserts generated by TAIL-PCR. In an F2 population of 230 plants, the BAC-end markers 42C23R and 6B7F flanked the xa13 locus. The probes 21H14F and 21H14R derived from BAC clone 21H14 were found to flank xa13 at a distance of 0.5 cM on either side, using a second F2 population of 132 plants. Thus, genetic mapping indicated that the contig and the 96-kb clone, 21H14, contained the xa13 locus.
Received: 15 August 1998 / Accepted: 29 September 1998 相似文献
12.
G. Zhang E. R. Angeles M. L. P. Abenes G. S. Khush N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(1-2):65-70
Bacterial blight (BB) caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the most serious diseases of rice. The recessive gene xa-13 confers resistance to Philippine race 6 of Xoo. To tag xa-13 with molecular markers, RAPD analysis was conducted with the combined use of near-isogenic lines and bulked segregant analysis. From the survey of 260 arbitrary 10-nucleotide primers, one primer (OPAC05) was detected to amplify specifically a 0.9-kb band from the DNA of susceptible plants. The distance between the RAPD marker OPAC05-900 and xa-13 was estimated to be 5.3 cM. The RAPD marker was then mapped on chromosome 8 using a mapping population of doubled haploid lines derived from the cross of IR64/Azucena. The linkage between RFLP markers and the RAPD marker was analyzed using an F2 population of 135 plants derived from a cross between a near-isogenic line for xa-13, IR66699-5-5-4-2, and IR24. No recombinants were found between RZ28 and CDO116 and their distance from xa-13 was estimated to be 4.8 cM. RG136 was located at 3.7 cM on the other side of xa-13. The mapping of xa-13 with closely linked DNA markers provides the basis for marker-aided selection for rice improvement.Department of Agronomy, South China Agricultural University, Guangzhou, China 相似文献
13.
K. Datta R. Velazhahan N. Oliva I. Ona T. Mew G. S. Khush S. Muthukrishnan S. K. Datta 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1138-1145
A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was
cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, ‘Chinsurah
Boro II’, ‘IR72’, and ‘IR51500’ were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos.
The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was
confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged
with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
Received: 11 August 1998 / Accepted: 26 August 1998 相似文献
14.
Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) 总被引:1,自引:1,他引:0
V. Jorge M. A. Fregene M. C. Duque M. W. Bonierbale J. Tohme V. Verdier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):865-872
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic
mapping of an F1 cross (TMS 30572 × CM 2177–2) led to the construction of the first molecular genetic map of cassava. To better understand
the genetics of resistance to CBB, we evaluated individuals of the F1 cross for CBB resistance by controlled greenhouse inoculations and visually assessed symptoms on days 7, 15, and 30 days
after inoculation, using a scale where 0 = no disease and 5 = maximum susceptibility. Five Xam strains were used: CIO-84, CIO-1, CIO-136, CIO-295, and ORST X-27. Area under the disease progress curve (AUDPC) was used
as a quantitative measure of resistance in QTL analysis by single-marker regression. Based on the AUDPC values, eight QTLs
(quantitative trait loci), located on linkage groups B, D, L, N, and X of the female-derived framework map, were found to
explain 9–20% of the phenotypic variance of the crop’s response to the five Xam strains. With the male-derived framework map, four QTLs on linkage groups G and C explained 10.7–27.1% of the variance. A
scheme to confirm the usefulness of these markers in evaluating segregating populations for resistance to CBB is proposed.
Received: 20 September 1999 / Accepted: 30 December 1999 相似文献
15.
Maruthasalam S Kalpana K Kumar KK Loganathan M Poovannan K Raja JA Kokiladevi E Samiyappan R Sudhakar D Balasubramanian P 《Plant cell reports》2007,26(6):791-804
Elite indica rice cultivars were cotransformed with genes expressing a rice chitinase (chi11) and a thaumatin-like protein (tlp) conferring resistance to fungal pathogens and a serine-threonine kinase (Xa21) conferring bacterial blight resistance, through particle bombardment, with a view to pyramiding sheath blight and bacterial
blight resistance. Molecular analyses of putative transgenic lines by polymerase chain reaction, Southern Blot hybridization,
and Western Blotting revealed stable integration and expression of the transgenes in a few independent transgenic lines. Progeny
analyses showed the stable inheritance of transgenes to their progeny. Coexpression of chitinase and thaumatin-like protein
in the progenies of a transgenic Pusa Basmati1 line revealed an enhanced resistance to the sheath blight pathogen, Rhizoctonia solani, as compared to that in the lines expressing the individual genes. A transgenic Pusa Basmati1 line pyramided with chi11, tlp, and Xa21 showed an enhanced resistance to both sheath blight and bacterial blight.
S. Maruthasalam and K. Kalpana have contributed to this article equally. 相似文献
16.
Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China 总被引:2,自引:0,他引:2
S. K. Katiyar Y. Tan B. Huang G. Chandel Y. Xu Y. Zhang Z. Xie J. Bennett 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(6-7):953-961
The Chinese rice cultivar Duokang #1 carries a single dominant gene Gm-6(t) that confers resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae Wood-Mason) known in China. Bulked segregant analysis was performed on progeny of a cross between Duokang #1 and the gall
midge-susceptible cultivar Feng Yin Zhan using the RAPD method. The RAPD marker OPM06(1400) amplified a locus linked to Gm-6(t). The locus was subsequently mapped to rice chromosome 4 in a region flanked by cloned RFLP markers RG214 and RG163. Fine
mapping of Gm-6(t) revealed that markers RG214 and RG476 flanked the gene at distances of 1.0 and 2.3 cM, respectively. Another gall midge
resistance gene, Gm-2, mapped previously to chromosome 4, is located about 16 cM from Gm-6(t), to judge by data from a segregating population derived from a cross between Duokang #1 and the Indian cultivar Phalguna
that carries Gm-2. We developed a PCR-based marker-assisted selection kit for transfer of the Gm-6(t) gene into Ming Hui 63 and IR50404, two parental lines commonly used in hybrid rice production in China. The kit contains
PCR primer pairs based on the terminal sequences of the RG214 and RG476 clones. Polymorphism between Duokang #1 and the hybrid
parental lines was found at these markers after digestion of the PCR products with specific restriction endonucleases. The
kit will accelerate introduction of gall midge resistance into hybrid rice in China.
Received: 18 May 2000 / Accepted: 9 March 2001 相似文献
17.
Particle-bombardment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests 总被引:16,自引:0,他引:16
Kexuan Tang Porntip Tinjuangjun Yanan Xu Xiaofen Sun John A. Gatehouse Pamela C. Ronald Huaxiong Qi Xinggui Lu Paul Christou Ajay Kohli 《Planta》1999,208(4):552-563
18.
Agrobacterium-mediated engineering for sheath blight resistance of indica rice cultivars from different ecosystems 总被引:3,自引:0,他引:3
K. Datta Z. Koukolíková-Nicola N. Baisakh N. Oliva S.K. Datta 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(6):832-839
A concise T-DNA element was engineered containing the rice class-I chitinase gene expressed under the control of CaMV35S and the hygromycin phosphotransferase gene (hph) as a selectable marker. The binary plasmid vector pNO1 with the T-DNA element containing these genes of interest was mobilized
to Agrobacterium
tumefaciens strain LBA4404 to act as an efficient donor of T-DNA in the transformation of three different indica rice cultivars from
different ecosystems. Many morphologically normal, fertile transgenic plants from these rice cultivars were generated after
Agrobacterium-mediated transformation using 3-week-old scutella calli as initial explants. Stable integration, inheritance and expression
of the chimeric chitinase gene were demonstrated by Southern blot and Western blot analysis of the transformants. Bioassay data showed that transgenic
plants can restrict the growth of the sheath blight pathogen Rhizoctonia solani. Bioassay results were correlated with the molecular analysis. Although we obtained similar results upon DNA-mediated transformation,
this report shows the potential of the cost-effective, simple Agrobacterium system for genetic manipulation of rice cultivars with a pathogenesis-related (PR) gene.
Received: 26 July 1999 / Accepted: 27 August 1999 相似文献
19.
Genetic relationships among Stylosanthes species as revealed by sequence-tagged site markers 总被引:1,自引:0,他引:1
J. Vander Stappen I. Weltjens S. Van Campenhout G. Volckaert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1054-1062
Nineteen sequence-tagged site (STS) primer pairs were designed on coding and non-coding regions in nine published Stylosanthes genes, which were mostly derived from cDNA. Direct sequencing of PCR products derived from genomic DNA allowed us to identify
introns and to design specific primers flanking these introns. The use of 24 STS primer pairs for the detection of intra-
and inter-specific variation in Stylosanthes based on size differences was tested on a core set of Stylosanthes species. Based on these results, 20 STS markers were selected to determine genetic relationships among 63 genotypes representing
24 Stylosanthes species. A total of 148 alleles were amplified and analyzed, resulting in a genetic similarity value ranging from 0.62 to
0.98 among the species. Based on cluster analysis, three main groups and three subgroups were determined, and most of the
species were classified unambiguously. Alloploid species were recognized by the occurrence of more than one allele per STS
marker, indicating fixed heterozygosity. Sixteen STS markers were useful for the identification of genotypes within a species.
Inter-species relationships, as revealed by STS, were in general agreement with previous morphological and molecular relationship
studies. These STS markers are useful as an additional tool for the identification of species, subspecies and genotypes in
Stylosanthes, with a view to plant conservation and breeding.
Received: 2 June 1998 / Accepted: 28 October 1998 相似文献
20.
Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32 总被引:7,自引:0,他引:7
Zenbayashi K Ashizawa T Tani T Koizumi S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):547-552
The rice cultivar Chubu 32 possesses a high level of partial resistance to leaf blast. The number and chromosomal location
of genes conferring this resistance were detected by restriction fragment length polymorphism (RFLP) linkage mapping and quantitative
trait locus (QTL) analysis. For the mapping, 149 F3 lines derived from the cross between rice cultivar Norin 29, with a low level of partial resistance, and Chubu 32 were used,
and their partial resistance to leaf blast was assessed in upland nurseries. A linkage map covering six chromosomes and consisting
of 36 RFLP markers was constructed. In the map, only one significant QTL (LOD>2.0) for partial resistance was detected on
chromosome 11. This QTL explained 45.6% of the phenotypic variation. The segregation ratio of the F3 lines was 3:1 for partial
resistance to susceptibility. These results suggest that the partial resistance in Chubu 32 is controlled by a major gene.
Received: 15 March 2001 / Accepted: 13 August 2001 相似文献