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Cryopreservation is likely the choice for long-term preservation of natural and engineered tissues, and high concentration multiple cryoprotective agents (CPAs) are usually used in such a process. To achieve high cell viability after cryopreservation, cells at all locations within the tissue must be protected properly by the CPAs during freezing. It is hence essential to know the distribution and concentration of CPAs within the tissue during multiple-CPA addition, to maximize cell survival and minimize tissue damage. In this work, a model to describe the CPA transport during multiple-CPA addition in a one-dimensional porous medium, as a simplified model of living tissue, was developed on the basis of the Maxwell-Stefan (M-S) equations. The UNIFAC and UNIQUAC models were used to evaluate the activity coefficients, and the Siddiqi-Lucas correlation was used for estimation of Maxwell-Stefan diffusivities. Simulations were carried out to examine the effect of temperature, tissue property, CPA type and the interactions between solutes on the CPA transport within construct during the CPA addition. It was found that these parameters, especially the interactions between the different CPA molecules, which was neglected before, significantly affect the transport of each individual CPA component. It is hence concluded that the traditional single-component analysis on the CPA diffusion is not adequate to quantify the multiple-CPA distribution in the tissue, particularly when the CPA concentrations are relatively high. 相似文献
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M J Walport 《BMJ (Clinical research ed.)》1987,294(6569):392-393
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Low sodium isocyanurate concentrations as a substitute to medium autoclaving in plant tissue culture
da Costa Urtiga Caio de Araújo Silva-Cardoso Inaê Mariê Araujo Figueiredo Sergio 《Plant Cell, Tissue and Organ Culture》2019,139(3):601-604
Plant Cell, Tissue and Organ Culture (PCTOC) - An optimization for a medium sterilization method, capable of substituting autoclaving, was developed using low concentrations of sodium isocyanurate... 相似文献
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LONGMUIR IS 《The Biochemical journal》1954,57(1):81-87
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Spermidine and spermine decrease betacyanin efflux from the discs of beet root storage tissue probably by stabilizing the cell membrane. The efflux reversal by spermine is not achieved at 47° probably due to irreversible membrane damage. Phytate alone has no effect on the efflux but reverses the effect of spermine. The destabilization produced by alcohol is reversed by spermine at low concentration but at high concentration both act synergistically to enhance the efflux. 相似文献
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An expression is derived which describes the transient distribution of solute diffusing into heterogeneous tissue from a fully-stirred
cylindrical region in which there has been a step change in solute concentration. This is envisaged as a model for the uptake
of drugs by vessel walls, although the same approach has been extended for estimating the mode of total uptake of substances
by annular specimens of tissue. Tissue is regarded as effectively composed of two phases—an extracellular (continuous) phase,
similar to water, and a dispersed phase comprising cells of irregular profile. In each phase the relevant mode of uptake is
taken as bulk diffusion rather than the permeation of a surface membrane. 相似文献
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B. A. Hills 《Bulletin of mathematical biology》1968,30(1):47-59
In this paper an expression is derived which describes the transient overall uptake of an inert solute by a section of tissue
excised with parallel faces and placed upon an impermeable base. The approach diverges from the conventional analyses for
perfused tissue (Morales and Smith,Bull. Math. Biophysics,6, 125–141, 1944;7, 47–99, 1945) because the extravascular zone is regarded as a heterogeneous diffusion medium. Account for this is taken by
regarding tissue as effectively composed of two phases—a continuous (extracellular) phase similar to water, and a dispersed
phase comprising cells of irregular profile. In both phases the relevant mode of uptake is taken as bulk diffusion rather
than surface permeation, thus emphasizing the influence of the internal geometry of the tissue upon its overall exchange response. 相似文献
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Eagle's basal medium as a defined medium for Mycoplasma studies 总被引:8,自引:0,他引:8
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A chemical method based upon chitin analysis is suitable for estimating growth of Colletotrichum lagenarium; it has been calibrated against total dry weight determination, in synthetic media. This method allows us to compare the growth of virulent and avirulent strains of this pathogenic fungus in culture on a medium containing cell walls, isolated from host tissues, as carbon and nitrogen sources. 相似文献
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《International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology》1988,15(6):665-672
Labeling of plasma transferrin with gallium was investigated to determine whether the gallium-transferrin complex could be effectively used as a macromolecular tracer in studies of capillary permeability using Positron Emission Tomography (PET). Three gallium-plasma preparations were tested and 2 h bio-distribution studies were performed in rats. The three preparations gave similar blood clearance and tissue distribution data, but the methods used for evaluating gallium-transferrin binding were found to be suboptimal. Gallium clearance from blood was biexponential with both components faster than that of 125I-albumin. Gallium distribution spaces in all tissues including intracerebral Walker-256 tumors were larger than those of albumin. These results indicate a relative instability of the gallium-transferrin complex in vivo, which appears to preclude its use as an acceptable radiolabeled protein for vascular permeability studies using PET. 相似文献
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A commercially available tissue culture medium has been proven capable of preserving dog kidney function for at least 24 hr after simple cooling. The advantages of using tissue culture medium as preservation fluid instead of plasma or albumin solutions from the infectious and immunological points of view are obvious. An in vitro study was completed using the tissue
Code (animal No.) | Perfusion time (br) | Perfusion pressure mm/Hg | Flow ml/min | Weight gain | pH | pO2 mm/Hg | Histological appearance |
1 | 24 | 70-60 systolic | 96 | 35 | 7.3 | 150–180 | Grossly normal |
2 | 24 | 45-40 diastolic | 108 | 30 | |||
3 | 24 | 96 | 30 | ||||
4 | 48 | 70-60 systolic | 80 | 35 | 7.3 | 150–180 | Grossly normal |
5 | 48 | 45-40 diastolic | 120 | 40 | 7.4 | ||
6 | 48 | 100 | 40 | ||||
7 | 72 | 70-60 systolic | 115 | 40 | 7.4 | 150–180 | Slight vacuolization of the tubular cells |
8 | 72 | 45-40 diastolic | 96 | 40 | |||
9 | 72 | 80 | 40 | ||||
10 | 24 | 70-60 systolic | 110 | 35 | 7.3 | 150–180 | Used for transplantation |
11 | 24 | 45-40 diastolic | 120 | 35 | |||
12 | 24 | 140 | 40 | ||||
13 | 24 | 100 | 30 | ||||
14 | 24 | 96 | 30 |