共查询到20条相似文献,搜索用时 15 毫秒
1.
J.D. Gee 《Journal of insect physiology》1977,23(1):137-143
A self-supporting colony of Glossina morsitans can be maintained by feeding flies through artificial membranes but only if a diet of defibrinated pig blood is used. The potassium concentration of pig serum was found to be higher than that of cow or rabbit serum. However, this did not affect the rate of diuresis which was lower after in vitro diets than after feeding on a live host.After feeding in vivo on rabbits, and in vitro on pig blood and cow blood, the concentrations of sodium and potassium in the haemolymph remained constant. After feeding on salt solutions and distilled water, on the other hand, it was found that the more the composition of the meal varied from that of blood serum the lower was the rate of diuresis and the more the concentrations of sodium and potassium in the haemolymph were displaced from their normal values. Large fluctuations in the concentrations of these ions resulted in the paralysis and death of the flies.The results demonstrate that the tsetse fly is able to reabsorb sodium from the primary urine produced by the Malpighian tubules during diuresis and it is suggested that this reabsorption of ions may lead to an increased circulation of water through the excretory system. 相似文献
2.
Female Anopheles freeborni discharge urine rapidly and copiously for a brief time after taking a meal of blood. This diuresis begins immediately upon cessation of feeding and continues for about 30 min at a constant rate. A decline in this rate follows and diuresis is completed by 50 min after feeding. This time-course of diuresis is independent of the size of the meal; diuresis after large meals occures at a higher rate, not over a longer time, than diuresis after small meals. Heat-stable and saline-soluble substances that induce rapid excretion by isolated Malpighian tubules can be extracted from the head and thoracic nervous system, suggesting the presence of a neurosecretory diuretic hormone similar to that found in other blood-sucking insects. Decapitation or section of the ventral nerve cord abolishes the rapid phase of diuresis after a bloodmeal. Therefore, in analogy to the situation in the tsetse fly, the head is required either as the source of a diuretic hormone or as link in the pathway that stimulates its release. 相似文献
3.
G. D. Wheelock D. H. Petzel J. D. Gillett K. W. Beyenbach H. H. Hagedorn 《Archives of insect biochemistry and physiology》1988,7(2):75-89
In previous studies we have presented evidence for the role of peptides, isolated from heads of the mosquito Aedes aegypti, in stimulating fluid secretion by isolated Malpighian tubules. In the present study we conducted experiments to investigate whether these peptides are involved in hormone-mediated diuresis after a blood meal. In vivo experiments showed that the head was required to maintain diuresis after the blood meal. Whereas feeding on blood triggered a prompt diuresis in the intact mosquito, subsequent decapitation caused a gradual, not an abrupt, decline in urine excretion rate. Hemolymph collected from mosquitoes fed blood significantly stimulated fluid secretion in vitro by isolated Malpighian tubules, whereas hemolymph from unfed or blood-fed decapitated mosquitoes did not. These results indicate that a diuretic factor was released into the hemolymph after a blood meal. This factor was not present in the hemolymph of decapitated females. We identified the head as a source of diuretic factors. Peptides isolated from a head extract by high-performance liquid chromatography, when injected into the hemocoel of blood-fed decapitated mosquitoes, triggered diuresis in vivo and also stimulated fluid secretion in isolated Malpighian tubules. These studies support the hypothesis that the head is a storage site for diuretic peptides that may be released after a blood meal to control diuresis. 相似文献
4.
Diuresis in the cabbage white butterfly, Pieris brassicae: Fluid secretion by the malpighian tubules
Susan W. Nicolson 《Journal of insect physiology》1976,22(10):1347-1356
The sudden weight loss at the pupal-adult ecdysis of the cabbage white butterfly, Peiris brassicae, is caused by a diuresis which greatly reduces the blood volume after emergence. Study of the Malpighian tubules as isolated preparations shows that only those of the adult insect possess the ability to secrete fluid at extremely fast rates when stimulated. In contrast to the fast-secreting tubules of bloodsucking insects, the mechanism of fluid secretion is not unusual, probably depending on the active transport of potassium ions. 相似文献
5.
Allatotropin Modulates Myostimulatory and Cardioacceleratory Activities in Rhodnius prolixus (Stal).
María José Villalobos-Sambucaro Alicia Nieves Lorenzo-Figueiras Fernando Luis Riccillo Luis Anibal Diambra Fernando Gabriel Noriega Jorge Rafael Ronderos 《PloS one》2015,10(4)
Haematophagous insects can ingest large quantities of blood in a single meal and eliminate high volumes of urine in the next few hours. This rise in diuresis is possible because the excretory activity of the Malpighian tubules is facilitated by an increase in haemolymph circulation as a result of intensification of aorta contractions combined with an increase of the anterior midgut peristaltic waves. It has been previously described that haemolymph circulation during post-prandial diuresis is stimulated by the synergistic activity of allatotropin (AT) and serotonin in the kissing bug Triatoma infestans; resulting in an increase in aorta contractions. In the same species, AT stimulates anterior midgut and rectum muscle contractions to mix urine and feces and facilitate the voiding of the rectum. Furthermore, levels of AT in midgut and Malpighian tubules increased in the afternoon when insects are getting ready for nocturnal feeding. In the present study we describe the synergistic effect of AT and serotonin increasing the frequency of contractions of the aorta in Rhodnius prolixus. The basal frequency of contractions of the aorta in the afternoon is higher that the observed during the morning, suggesting the existence of a daily rhythmic activity. The AT receptor is expressed in the rectum, midgut and dorsal vessel, three critical organs involved in post-prandial diuresis. All together these findings provide evidence that AT plays a role as a myoregulatory and cardioacceleratory peptide in R. prolixus. 相似文献
6.
《Journal of insect physiology》1991,37(6):447-452
Malpighian tubules of Namib Desert tenebrionid beetles of the genus Onymacris are strongly stimulated by homogenates of the corpora cardiaca. The corpora cardiaca of other arid-adapted tenebrionids also contain diuretic material. Biogenic amines, which could be released during the preparation of corpora cardiaca extracts, do not stimulate fluid secretion in tubules of Onymacris rugatipennis. The diuretic factor in corpora cardiaca extracts is stable to boiling and to incubation with pronase. HPLC separation of the corpora cardiaca of O. rugatipennis gives a single region with diuretic activity in both secretory and electrical bioassays. Diuretic activity can not be detected in the haemolymph of Onymacris, and injection of corpora cardiaca extracts into the beetles does not cause diuresis. Simultaneous injection of corpora cardiaca and the dye amaranth shows that the most of the dye transported by the Malpighian tubules moves anteriorly into the midgut, indicating fluid recycling by this route. The most likely function for this “diuretic hormone” is clearance of metabolic wastes from the haemolymph. 相似文献
7.
8.
Fernanda Stanisçuaski Victoria Te Brugge Ian Orchard 《Journal of insect physiology》2009,55(3):255-263
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to the release of a 10-kDa peptide by cathepsin-like enzymes in the insect's midgut. Urease causes a decrease in post-feeding weight loss in Rhodnius prolixus, suggesting an effect on water balance. To investigate how this impairment occurs, we have evaluated the action of JBU and the urease-derivated peptide Jaburetox-2Ec on R. prolixus Malpighian tubules and also investigated the involvement of second messengers. JBU and Jaburetox-2Ec affect serotonin-induced secretion from Malpighian tubules. This effect is not cAMP-dependent, but the Jaburetox-2Ec effect is cGMP-dependent. Eicosanoid metabolites and calcium ions appear to be involved in JBU effect on diuresis, but are not involved in the action of Jaburetox-2Ec. Jaburetox-2Ec, but not JBU, causes a change in the transepithelial potential of the tubules. Canatoxin has a similar effect on tubules secretion, decreasing the secretion rate, but the urease from Helicobacter pylori has no significant effect. These data are helpful in our understanding of the actions of ureases and derived peptides on insects, and also reinforces the potential use of these proteins as biopesticides. 相似文献
9.
J.S. Ryerse 《Journal of insect physiology》1978,24(4):315-319
Developmental changes in Malpighian tubule fluid transport were studied using in vitro and in situ preparations from timed larval, pupal and adult skipper butterflies (Calpodes ethlius). The ability to transport fluid and the rate of fluid secretion depend on the developmental stage and the physiological state. Larval tubules are permanently switched on, do not require a diuretic hormone and transport fluid at an increasing rate as the larvae feed and grow. Fluid transport continues at larval-larval moults but is switched off 24 hr before pupal ecdysis. No secretory activity occurs during the first half of the pupal stage when the tubules are remodelled for adult function. The resumption of fluid transport midway through the pupal stage is in preparation for a rapid diuresis at adult emergence. High rates of fluid secretion are associated with feeding or drinking in adults. 相似文献
10.
11.
The Malpighian (renal) tubules play important roles in ionic and osmotic homeostasis in insects. In Lepidoptera, the Malpighian tubules are structurally regionalized and the concentration of Na+ and K+ in the secreted fluid varies depending on the segment of tubule analyzed. In this work, we have characterized fluid and ion (Na+, K+, H+) transport by tubules of the larval stage of the cabbage looper Trichoplusia ni; we have also evaluated the effects of fluid secretion inhibitors and stimulants on fluid and ion transport. Ramsay assays showed that fluid was secreted by the iliac plexus but not by the yellow and white regions of the tubule. K+ and Na+ were secreted by the distal iliac plexus (DIP) and K+ was reabsorbed in downstream regions. The fluid secretion rate decreased > 50% after 25 μM bafilomycin A1, 500 μM amiloride or 50 μM bumetanide was added to the bath. The concentration of K+ in the secreted fluid did not change, whereas the concentration of Na+ in the secreted fluid decreased significantly when tubules were exposed to bafilomycin A1 or amiloride. Addition of 500 μM cAMP or 1 μM 5-HT to the bath stimulated fluid secretion and resulted in a decrease in K+ concentration in the secreted fluid. An increase in Na+ concentration in the secreted fluid was observed only in cAMP-stimulated tubules. Secreted fluid pH and the transepithelial electrical potential (TEP) did not change when tubules were stimulated. Taken together, our results show that the secretion of fluid is carried out by the upper regions (DIP) in T. ni Malpighian tubules. Upper regions of the tubules secrete K+, whereas lower regions reabsorb it. Stimulation of fluid secretion is correlated with a decrease in the K+/Na+ ratio. 相似文献
12.
In vitro preparations of locust Malpighian tubules can conveniently be made by a new technique in which the alimentary canal to which the tubules attach is removed from the insect and set up in Ringer's solution under liquid paraffin. Such Malpighian tubules will secrete a fluid iso-osmotic to the bathing fluid at a steady rate of about 1 to 2 nl min?1 for some hours. The secreted fluid is rich in potassium ions, the lumen is at a potential positive to that of the bathing solution, and the rate of secretion can be controlled by changing the potassium concentration of the bathing fluid. It seems likely, therefore, that an active transport of potassium drives secretion ny locust Malpighian tubules. The secreted fluid contains an elevated concentration of phosphate ions. The Malpighian tubules will secrete at a high rate in a chloride-free phosphate-based solution. The rate of fluid secretion can be increased by treatment with cyclic AMP but 5-hydroxytryptamine has no such effect. 相似文献
13.
14.
U Atzbacher F Hevert E Weber-Von Grotthuss A Wessing 《Journal of insect physiology》1974,20(10):1989-1997
The sour dyes azocarmine and indigocarmine are excreted through the Malpighian tubules and the midgut after injection into the body cavity of third instar Drosophila hydei larvae. After injection, the other organs are free of dyes. The epithelium of the midgut does not allow orally applied dyes to pass into the haemolymph. Ouabain diminishes significantly the content of dyes in the cavity of the Malpighian tubules and of the midgut. The maximal concentration of azocarmine decreases in the Malpighian tubules to about 65 per cent and in the midgut to about 70 per cent. Indigocarmine decreases in the Malpighian tubules to about 55 per cent. The content of indigocarmine of the midgut does not change significantly after ouabain injections. As ouabain inhibits active ion transport, the decrease of the concentration of dyes is seen as proof of the coupling of active ion transport processes and of excretion of the dyes. Moreover, this decrease points to an ouabain-sensitive transport mechanism, which is localized in the epithelia of the Malpighian tubules and midgut. 相似文献
15.
Andrea Iaboni G. Mark Holman Ronald J. Nachman I. Orchard G. M. Coast 《Cell and tissue research》1998,294(3):549-560
The distribution of a CRF-related diuretic peptide (Musca-DP) and the diuretic/myotropic insect myokinins in the central nervous system of larval and adult houseflies was investigated using antisera raised against Locusta-DH and leucokinin-I, respectively. Two separate, small populations of immunoreactive neurons are present in the brain and fused thoracic-abdominal ganglion mass. There is no evidence for these immunoreactivities being colocalised either within single neurons or at neurohaemal release sites. Crude extracts of tissues containing immunoreactive material increase fluid secretion by isolated Malpighian tubules from adult flies. Diuretic activity is highest in tissues containing myokinin-immunoreactive material. Consistent with this observation, myokinin analogues produce a four- to five-fold increase in fluid secretion, which is more than twice the response to Musca-DP. These effects are mimicked by treatments that increase intracellular calcium and cyclic AMP, respectively. When tested at threshold concentrations, the two classes of diuretic peptide act synergistically to accelerate tubule secretion, and their separate localisation may be important for the precise control of diuresis. 相似文献
16.
In vivo increase in haemolymph volume of canavanine-treated locusts substantiates our previous in vitro findings that canavanine inhibits fluid secretion by locust Malpighian tubules. Furthermore when diuretic hormone is applied in vivo after canavanine treatment haemolymph volume is drastically reduced below levels retained in locusts untreated with canavanine. Again this is in accord with canavanine potentiation of semi-isolated Malpighian tubules and enhanced fluid secretion in vitro. The response is specific to canavanine; compounds similar in structure (arginine, argininic acid, citrulline, canaline, ornithine and homoserine) have no effect on the rate of fluid secreted by Malpighian tubules. Only partial competition is obtained with uridine homoserine. 相似文献
17.
Young females of seven species of Florida mosquitoes were fed a meal of dog blood infected with Dirofilaria immitis to repletion to study the physiological mechanisms which control susceptibility and resistance in these mosquitoes. Various species of mosquitoes showed different grades of susceptibility. In all mosquitoes, microfilariae reached the midgut immediately after ingestion. Their movement from midgut to the specific host tissue—the Malpighian tubules—was either facilitated or inhibited depending on the presence or absence of anticoagulins in the salivary glands of these mosquitoes. In Anopheles quadrimaculatus, Aedes taeniorhynchus, and Aedes sollicitans, microfilariae move freely from the midgut to the Malpighian tubules, because of the presence of substantial amounts of anticoagulins in their salivary glands, and 30 to 60 mf/female developed normally to an infective stage. Very few microfilariae reached the tubules of Mansonia titillans as most of them were defaecated within a very short time after ingestion. In Aedes aegypti, Culex nigripalpus, and Culex pipiens quinquefasciatus movement of microfilariae from the midgut to the Malpighian tubules was obstructed by the coagulation of blood soon after ingestion. Coagulation of blood was followed by formation of oxyhaemoglobin crystals in C. nigripalpus and C. p. quinquefasciatus. It is suggested that secretions from symbiotic bacteria in the midgut of these mosquitoes lyse ingested red blood cells, and the released haemoglobin is oxidized to oxyhaemoglobin crystals which hinder the further movement of microfilariae and kill them.Microfilariae developed normally in A. quadrimaculatus, thus making them potentially the most susceptible mosquitoes, even though these mosquitoes did not survive to be effective potential vectors. A few microfilariae or their later developmental stages were melanized in the tubules of most A. sollicitans and A. taeniorhynchus, but the numbers of melanized stages were too few to affect the vectoring potentials of these species. In 20 per cent of A. sollicitans, 60 per cent of M. titillans, and ca. 80 per cent A. aegypti substantial numbers of the microfilariae after reaching the Malpighian tubules did not advance beyond the prelarval stage, and very few microfilariae developed successfully in the remaining mosquitoes. Very few microfilariae reached the Malpighian tubules of a small percentage of C. nigripalpus and C. p. quinquefasciatus and developed normally. The vectoring potentials of A. sollicitans, M. titillans, A. aegypti, and both Culex species were greatly hampered. These studies suggested that host-specificity of mosquitoes to D. immitis infection is controlled by the presence or absence of secondary physiological factors in their digestive tracts or in the Malpighian tubules. 相似文献
18.
David M. Phillips 《The Journal of cell biology》1966,30(3):477-497
Although 9-membered centrioles are found in somatic tissues of Sciara, the centriole which lies at the spindle pole of the second meiotic division in male Sciara is composed of a row of approximately 70 short tubules in an oval array. Shortly after telophase of this unequal division, in the daughter cell destined to undergo spermiogenesis, microtubules become confluent with the tubules of the centriole. These tubules have the same density as other cytoplasmic microtubules after glutaraldehyde-OsO4 fixation and, like them, are not preserved with OsO4 fixation. As the centriole, now with approximately 70 attached, posteriorly directed, doublet tubules, migrates from the polar to the apolar end of the nucleus to take a final position in an oval groove which forms in the nuclear envelope, the tubules lengthen and become demonstrable after OsO4 fixation and more electron opaque than other cytoplasmic microtubules following glutaraldehyde-OsO4 fixation. Later, a singlet tubule appears peripherally to each doublet of the oval and 4 "arms" develop at specific sites on the tubules. Posteriorly, where the oval of tubules becomes discontinuous and forms a spiral, the arrangement of arms is different and the singlet tubules are lacking. Dense solid bodies develop inside this odd flagellum and become enclosed by a smooth double membrane. A single mitochondrial derivative has three components: a central area of homogeneous, moderately electron-opaque, proteinaceous material; a peripheral ring of cristae; and a crystalloid which is specifically oriented with respect to the flagellar tubules. 相似文献
19.
Ruiz-Sanchez E Van Walderveen MC Livingston A O'Donnell MJ 《Journal of insect physiology》2007,53(10):1034-1045
The organic anion salicylate is a plant secondary metabolite that protects plants against phytophagous insects. In this study, a combination of salicylate-selective microelectrodes and a radioisotope tracer technique was used to study the transepithelial transport of salicylate by the Malpighian tubules of 10 species of insects from five orders. Our results show that salicylate is transported into the lumen of the Malpighian tubules in all the species evaluated, except Rhodnius prolixus. The transepithelial transport of salicylate by the Malpighian tubules of Drosophila simulans, Drosophila erecta, Drosophila sechellia, and Acheta domesticus was saturable, Na+-dependent and inhibited by α-cyano-4-hydroxycinnamic acid. This transport system resembles that previously found in tubules of Drosophila melanogaster. In contrast, transepithelial transport of salicylate by Malpighian tubules of Tenebrio molitor, Plagiodera versicolora, Aedes aegypti, and Trichoplusia ni was unaffected by Na+-free bathing saline. The presence of both salicylate and salicylate metabolites in the secreted fluid samples from the Malpighian tubules of A. domesticus, R. prolixus, T. molitor, and T. ni indicates that insect Malpighian tubules may both transport and metabolize salicylate. The highest capacities to rid the hemolymph of salicylate were found in T. molitor, P. versicolora and Drosphila spp. Our results suggest that transport of salicylate by the Malpighian tubules might contribute to elimination of this organic anion from the hemolymph, particularly in some species that encounter high levels of organic anion in the diet. 相似文献
20.
The ionic dependencies of stimulated and unstimulated Locusta tubules have been studied. K+, Na+, Cl? are essential to both basal and stimulated secretion. K+ is secreted against a concentration gradient in unstimulated tubules. In response to diuretic hormone or cAMP application, there is a dramatic influx of K+ into the lumen. A high level of Na+ and Cl? in the bathing medium is required to allow maximal fluid secretion. The tubules show an apparent impermeability to Na+; its concentration in the secreted fluid is always much less than in the bathing medium. If Na+ is omitted from the medium and excess K+ added (80 mM K), then although basal secretion occurs (2.5 nl/min), the tubules fail to respond to stimulation. Clearly Na+ has an important indirect role to play in stimulated fluid secretion. 相似文献