Assessing Habitat Suitability for Juvenile Atlantic Salmon in Relation to In‐Stream Restoration and Discharge Variability

In‐stream restoration often aims at increasing the availability of the stream habitat suitable for salmonid fishes, thus creating potential for increased fish abundance. We assessed the success of in‐stream restoration of River Kiiminkijoki, northern Finland, by combining River2D habitat hydraulic modeling and fish density monitoring at the same sites, with data from multiple restored and reference reaches for 3 years both before and after restoration. We modeled the effects of restoration on the area suitable (weighted usable area, WUA) for juvenile Atlantic salmon from post‐hatching to age‐1 fish. Wetted width in the restored reaches increased by 8.1% on average compared with only ?0.2% change in the reference reaches. Habitat time series across 10 years showed significant increases in the amount of suitable habitat under summer conditions for both age‐0 and age‐1 salmon. However, improvement of overwintering habitats was marginal or nonexistent. Densities of age‐1 salmon showed no response to restoration. Low river discharge during the winter was correlated with low salmon densities the following summer. It thus appears that variability in wintertime discharge, and associated high interannual variation of WUA values, overrode the almost 20% increase in average post‐ versus pre‐restoration summertime WUA. Our study shows that the combination of hydraulic modeling and biological monitoring is a promising approach to stream restoration assessment.     

Adult aquatic insects: Potential contributors to riparian food webs in Australia's wet–dry tropics

Abstract Changes in the abundance and biomass of aquatic and terrestrial aerial insects with distance (mid‐stream, 0, 10–15 and 160 m) from lowland streams were examined across the dry season landscape in Kakadu National Park, northern Australia. Malaise traps and sticky intercept traps were used to sample the insects at four streams, spaced over an area of 1650 km². Malaise and intercept catches were dominated by Diptera (flies and midges), both numerically and by biomass. Chironomid midges were the most abundant taxon, making up 43.4 and 51.0% of the malaise and intercept trap catches, respectively. However, most chironomids were small (less than 3 mm body length), contributing 34.9% to intercept trap biomass, but only 5.2% in malaise traps. Ceratopogonid midges and caddisflies (Trichoptera) accounted for most of the remaining adult aquatic insects. Major terrestrial components were Diptera and Hymenoptera in malaise traps and Coleoptera and Diptera in intercept traps. The total abundance and biomass of insects were much greater over streams and along the water's edge than in riparian (10–15 m) and savanna (160 m) habitats primarily because of the presence of large numbers of adult aquatic insects. The abundance and biomass of terrestrial insects in malaise traps showed no relationship with distance, but intercept trap catches suggested slightly greater abundances over the water and at the water's edge. The great abundance of aquatic insects relative to terrestrial insects close to streams suggests that they have the potential to be an important component of the diets of riparian insectivores, and predation may be an important pathway by which aquatic nutrients and energy are moved into terrestrial food webs.     

Post‐Project Appraisals of River Restoration in Advanced University Instruction

Post‐project appraisals (PPAs) are systematic assessments of built restoration projects, which provide feedback on performance of restoration approaches to improve future restoration efforts. Unfortunately, most restoration projects are not subject to systematic assessment because of lack of institutional arrangements to sustain long‐term evaluation and the orientation of most funding agencies towards project implementation rather than “studies.” As semester‐long courses on river restoration increasingly appear in university curricula at the graduate and advanced undergraduate level, independent student research projects for such courses can provide a mechanism for building a database of PPAs (and components thereof) and providing the students with a powerful learning experience. In two UC Berkeley courses, we require independent student projects involving original field research, peer review of first drafts, instructor (and often outside) review of second drafts, and presentation of results to a public symposium. Since 1995, the revised, final papers have been added to the University of California library, where they constitute one of the largest collections of restoration‐related studies currently available for any region: over 300 restoration‐related studies, of which 80 are PPAs or components thereof. Since 2003, the papers have been posted on‐line, with 40,000 full text downloads through 2010. Some term projects have directly influenced river restoration programs, inducing changes in salmon habitat enhancement project design, documenting failure of projects based on inappropriate restoration approaches, and contributing to systematic assessments of step‐pool and compound channel designs in urban areas. Student evaluations cite the term projects as valuable learning experiences.     

Insights from a Cross‐Disciplinary Seminar: 10 Pivotal Papers for Ecological Restoration

Restoration ecology is a deepening and diversifying field with current research incorporating multiple disciplines and infusing long‐standing ideas with fresh perspectives. We present a list of 10 recent pivotal papers exemplifying new directions in ecological restoration that were selected by students in a cross‐disciplinary graduate seminar at the University of California, Berkeley. We highlight research that applies ecological theory to improve restoration practice in the context of global change (e.g. climate modeling, evaluation of novel ecosystems) and discuss remaining knowledge gaps. We also discuss papers that recognize the social context of restoration and the coupled nature of social and ecological systems, ranging from the incorporation of cultural values and Traditional Ecological Knowledge into restoration, to the consideration of the broader impacts of markets on restoration practices. In addition, we include perspectives that focus on improving communication between social and natural scientists as well as between scientists and practitioners, developing effective ecological monitoring, and applying more integrated, whole‐landscape approaches to restoration. We conclude with insights on recurrent themes in the papers regarding planning restoration in human‐modified landscapes, application of ecological theory, improvements to restoration practice, and the social contexts of restoration. We share lessons from our cross‐disciplinary endeavor, and invite further discussion on the future directions of restoration ecology through contributions to our seminar blog site http://restecology.blogspot.com .     

Impacts of an invasive willow (Salix × rubens) on riparian bird assemblages in south‐eastern Australia

We explored how a woody plant invader affected riparian bird assemblages. We surveyed 15 200‐m‐long transects in riparian zones in a much‐changed landscape of eastern Victoria, Australia. Abundance, species‐richness, foraging‐guild richness and composition of birds were compared in transects in three habitat types: (i) riparian zones dominated by the invasive willow Salix × rubens; (ii) riparian zones lined with native woody species; and (iii) riparian zones cleared of almost all woody vegetation. We also measured abundance and richness of arthropods and habitat structure to explore further the effects of food resources and habitat on the avifauna. We observed 67 bird species from 14 foraging guilds. Native riparian transects had more birds, bird species and foraging guilds than willow‐invaded or cleared transects. Habitat complexity increased from cleared to willow‐invaded to native riparian transects, as did abundance of native and woodland‐dependent birds. Native shrub and trees species had more foliage and branch‐associated arthropods than did willows, consistent with a greater abundance and variety of foraging guilds of birds dependent on this resource. Willow spread into cleared areas is unlikely to facilitate greatly native bird abundance and diversity even though habitat complexity is increased. Willow invasion into the native riparian zone, by decreasing food resources and altering habitat, is likely to reduce native bird biodiversity and further disrupt connectivity of the riparian zone.     

In‐stream habitat and macroinvertebrate responses to riparian corridor length in rangeland streams

Conservation and restoration of riparian vegetation in agricultural landscapes has had mixed success at protecting in‐stream habitat, potentially due to the mismatch between watershed‐scale impacts and reach‐scale restoration. Prioritizing contiguous placement of small‐scale restoration interventions may effectively create larger‐scale restoration projects and improve ecological outcomes. We performed a multi‐site field study to evaluate whether greater linear length of narrow riparian tree corridors resulted in measurable benefits to in‐stream condition. We collected data at 41 sites with varying upstream tree cover nested within 13 groups in rangeland streams in coastal northern California, United States. We evaluated the effect of riparian tree corridor length on benthic macroinvertebrate communities, as well as food resources, water temperature, and substrate size. Sites with longer riparian corridors had higher percentages of invertebrates sensitive to disturbance (including clingers and EPT taxa) as well as lower water temperatures and less fine sediment, two of the most important aquatic stressors. Despite marked improvement, we found no evidence that macroinvertebrate communities fully recovered, suggesting that land use continued to constrain conditions. The restoration of long riparian corridors may be an economically viable and rapidly implementable technique to improve habitat, control sediment, and counter increasing water temperatures expected with climate change within the context of ongoing land use.     

Development of a Tool to Facilitate Participation of Maori in the Management of Stream and River Health

A cultural health index (CHI) for streams was developed in a program of collaborative research involving members of Ngai Tahu (an iwi [tribe] within the South Island of New Zealand) and ecologists at Otago University. The aim was to provide a tool for effective participation of Maori in resource management decisions. Five cultural values are of central importance to the nature of the CHI: mauri (spiritual life force), mahinga kai (traditional resource harvesting), kaitiakitanga (guardianship obligation), ki uta ki tai (mountains-to-the-sea holistic philosophy), and wai taonga waters that are treasured). The CHI has three components. Forty-six stream sites in two culturally important river catchments were first classified according to whether there is a traditional association with Maori. The second component assessed the historical and contemporary mahinga kai status of the site, including questions of legal and physical access. The third component was a Cultural Stream Health Measure (CSHM) that encapsulates indicators of catchment, riparian, and instream condition in a manner that is consistent with Maori values. The CSHM was found to be significantly correlated with western measures of stream health commonly used in New Zealand (Macroinvertebrate Community Index, Stream Health Monitoring and Assessment Kit), and performed at least as well in encapsulating the relationship between land development and stream health. We describe a multistep process by which other indigenous people can develop a cultural ecosystem health measure, and then use the tool to ensure a substantial role in decision making with the agency in charge.     

Applying ecological site concepts and state‐and‐transition models to a grazed riparian rangeland

Ecological sites and state‐and‐transition models are useful tools for generating and testing hypotheses about drivers of vegetation composition in rangeland systems. These models have been widely implemented in upland rangelands, but comparatively, little attention has been given to developing ecological site concepts for rangeland riparian areas, and additional environmental criteria may be necessary to classify riparian ecological sites. Between 2013 and 2016, fifteen study reaches on five creeks were studied at Tejon Ranch in southern California. Data were collected to describe the relationship between riparian vegetation composition, environmental variables, and livestock management; and to explore the utility of ecological sites and state‐and‐transition models for describing riparian vegetation communities and for creating hypotheses about drivers of vegetation change. Hierarchical cluster analysis was used to classify the environmental and vegetation data (15 stream reaches × 4 years) into two ecological sites and eight community phases that comprised three vegetation states. Classification and regression tree (CART) analysis was used to determine the influence of abiotic site variables, annual precipitation, and cattle activity on vegetation clusters. Channel slope explained the greatest amount of variation in vegetation clusters; however, soil texture, geology, watershed size, and elevation were also selected as important predictors of vegetation composition. The classification tree built with this limited set of abiotic predictor variables explained 90% of the observed vegetation clusters. Cattle grazing and annual precipitation were not linked to qualitative differences in vegetation. Abiotic variables explained almost all of the observed riparian vegetation dynamics—and the divisions in the CART analysis corresponded roughly to the ecological sites—suggesting that ecological sites are well‐suited for understanding and predicting change in this highly variable system. These findings support continued development of riparian ecological site concepts and state‐and‐transition models to aid decision making for conservation and management of rangeland riparian areas.     

Friends in Low Places: Responses of a Benthic Stream Fish to Intra‐Prey‐Guild Alarm Cues

Many aquatic species produce chemical alarm cues that serve as a warning to nearby conspecifics. In mixed‐species aggregations, individuals may also benefit by ‘eavesdropping’ on the chemical alarm cues of other species that are in the same prey guild. Rainbow Darters (Etheostoma caeruleum) are benthic fish that co‐occur with native Ozark Minnows (Notropis nubilus), recently introduced Western Mosquitofish (Gambusia affinis), and native Oklahoma Salamanders (Eurycea tyrnerensis), all of whom are vulnerable to the same predators. We tested the responses of darters to the damage‐released alarm cues of conspecifics (positive control), minnows, and mosquitofish; alarm cues from Bumblebee Gobies (Brachygobius doriae) served as a negative (allopatric) control. We also tested the response of sympatric and allopatric darters to the damage‐released alarm cues of Oklahoma Salamander. Darters exhibited a fright response to conspecific and minnow alarm cues, but not to cues from mosquitofish or gobies. Lack of response to mosquitofish cues could be because they are introduced or because they typically occur higher in the water column than darters. Darters that were sympatric with the salamander exhibited a fright response to the alarm cues of the salamander, while allopatric darters did not. Rainbow Darters can develop responses to the alarm cues of syntopic species (minnows and Oklahoma Salamander) within their prey guild.     

Ecological immunology in a fluctuating environment: an integrative analysis of tree swallow nestling immune defense

Evolutionary ecologists have long been interested by the link between different immune defenses and fitness. Given the importance of a proper immune defense for survival, it is important to understand how its numerous components are affected by environmental heterogeneity. Previous studies targeting this question have rarely considered more than two immune markers. In this study, we measured seven immune markers (response to phytohemagglutinin (PHA), hemolysis capacity, hemagglutination capacity, plasma bactericidal capacity, percentage of lymphocytes, percentage of heterophils, and percentage of eosinophils) in tree swallow (Tachycineta bicolor) nestlings raised in two types of agro‐ecosystems of contrasted quality and over 2 years. First, we assessed the effect of environmental heterogeneity (spatial and temporal) on the strength and direction of correlations between immune measures. Second, we investigated the effect of an immune score integrating information from several immune markers on individual performance (including growth, mass at fledging and parasite burden). Both a multivariate and a pair‐wise approach showed variation in relationships between immune measures across years and habitats. We also found a weak association between the integrated score of nestling immune function and individual performance, but only under certain environmental conditions. We conclude that the ecological context can strongly affect the interpretation of immune defenses in the wild. Given that spatiotemporal variations are likely to affect individual immune defenses, great caution should be used when generalizing conclusions to other study systems.     

Diet and a developmental time constraint alter life‐history trade‐offs in a caddis fly (Trichoptera: Limnephilidae)

Environmental factors influence variation in life histories by affecting growth, development, and reproduction. We conducted an experiment in outdoor mesocosms to examine how diet and a time constraint on juvenile development (pond‐drying) influence life‐history trade‐offs (growth, development, adult body mass) in the caddis fly Limnephilus externus (Trichoptera: Limnephilidae). We predicted that: (1) diet supplementation would accelerate larval growth and development, and enhance survival to adulthood; (2) pond‐drying would accelerate development and increase larval mortality; and (3) the relationship between adult mass and age at maturity would be negative. Diet supplementation did lead to larger adult mass under nondrying conditions, but did not significantly alter growth or development rates. Contrary to predictions, pond‐drying reduced growth rates and delayed development. The slope (positive or negative) of the female mass–age at maturity relationship depended on interactions with diet or pond‐drying, but the male mass–age relationship was negative and independent of treatment. Our results suggest that pond‐drying can have negative effects on the future fitness of individuals by increasing the risk of desiccation‐induced, pre‐reproductive mortality and decreasing adult body size at maturity. These negative effects on life history cannot be overcome with additional nutritional resources in this species. © 2008 The Linnean Society of London, Biological Journal of the Linnean Society, 2008, 95 , 495–504.     

Evaluating a Large‐Scale Eelgrass Restoration Project in the Chesapeake Bay

Approximately 90,000 shoots of eelgrass (Zostera marina) were planted over 3 years (2003–2005) at Piney Point (PP) in the lower Potomac River estuary in the Chesapeake Bay (mid‐Atlantic coast of North America) following 3 years of habitat evaluation using a Preliminary Transplant Suitability Index (PTSI) and test plantings. Initial survival was high for the 2003 and 2004 plantings; however, most of the eelgrass died during the summer following the fall planting. Habitat quality and restoration success were monitored for the 2005 plantings and compared to a nearby restoration site (St. George Island [SGI]). Eelgrass planted at PP in the fall of 2005 declined through the summer of 2006 with some recovery in the spring of 2007, but was gone by the end of the summer of 2007. The summer decline from late July to mid‐August of 2006 coincided with water temperatures greater than 30°C, hypoxic oxygen (0–3 mg/L) concentrations, and low percent light at leaf level (PLL < 15%). Epiphyte loads were much heavier at PP than at SGI, despite similar water quality. We suggest that this was the result of higher wave exposure at PP. All of these factors are likely to have contributed to the mortality of the 2005 plantings. Submerged aquatic vegetation habitat quality based on the PTSI, median PLL during the growing season, and test plantings did not explain the decline of the plantings. Restoration site selection criteria should be expanded to include the effects of wave exposure on self‐shading and epiphyte loads, and the potential for both short‐term exposures to stressful conditions and long‐term changes in habitat quality.     

Planning for Implementation: Landscape‐Level Restoration Planning in an Agricultural Setting

The conservation of biodiversity in highly fragmented landscapes often requires large‐scale habitat restoration in addition to traditional biological conservation techniques. The selection of priority restoration sites to support long‐term persistence of biodiversity within landscape‐scale projects however remains a challenge for many restoration practitioners. Techniques developed under the paradigm of systematic conservation planning may provide a template for resolving these challenges. Systematic conservation planning requires the identification of conservation objectives, the establishment of quantitative targets for each objective, and the identification of areas which, if conserved, would contribute to meeting those targets. A metric developed by systematic conservation planners termed “irreplaceability” allows for analysis and prioritization of such conservation options, and allows for the display of analysis results in a way that can engage private landowners and other decision makers. The process of systematic conservation planning was modified to address landscape‐level restoration prioritization in southern Ontario. A series of recent and locally relevant landscape ecology studies allowed the identification of restoration objectives and quantitative targets, and a simple algorithm was developed to identify and prioritize potential restoration projects. The application of an irreplaceability analysis to landscape‐level restoration planning allowed the identification of varying needs throughout the planning region, resulting from underlying differences in topography and settlement patterns, and allowed the effective prioritization of potential restoration projects. Engagement with rural landowners and agricultural commodity groups, as well as the irreplaceability maps developed, ultimately resulted in a substantial increase in the number and total area of habitat restoration projects in the planning region.     

Responses of riparian trees and shrubs to flow regulation along a boreal stream in northern Sweden

1. Flow dynamics is a major determinant of riparian plant communities. Therefore, flow regulation may heavily affect riparian ecosystems. Despite the large number of dams worldwide, little specific information is available on the longitudinal impacts of dams on vegetation, for example how far downstream and at what degree of regulation a dam on a river can influence riparian woodlands. 2. We quantified the long‐term responses of riparian trees and shrubs to flow regulation by identifying their lateral distribution and habitat conditions along a boreal river in northern Sweden that has been regulated by a single dam since 1948. The regulation has reduced annual flow fluctuations, this effect being largest at the dam, downstream from which it progressively decreases following the entrance of free‐flowing tributaries. 3. We related changes in the distribution patterns, composition, abundance and richness of tree and shrub species to the degree of regulation along the river downstream from the dam. Regulation has triggered establishment of trees and shrubs closer to the channel, making it possible to measure ecological impacts of flow regulation as differences in vegetation attributes relative to the positions of tree and shrub communities established before and after regulation. 4. Trees and shrubs had migrated towards the mid‐channel along the entire study reach, but the changes were largest immediately downstream of the dam. Shrubs were most impacted by flow regulation in terms of lateral movement, but the effect on trees extended furthest downstream. 5. The species composition of trees progressively returned to its pre‐regulation state with distance downstream, but entrance of free‐flowing tributaries and variation in channel morphology and substratum caused local deviations. Species richness after regulation increased for trees but decreased for shrubs. The changes in species composition and richness of trees and shrubs showed no clear downstream patterns, suggesting that other factors than the degree of regulation were more important in governing life form.     

Responses of insect herbivores and herbivory to habitat fragmentation: a hierarchical meta‐analysis

Loss and fragmentation of natural habitats can lead to alterations of plant–animal interactions and ecosystems functioning. Insect herbivory, an important antagonistic interaction is expected to be influenced by habitat fragmentation through direct negative effects on herbivore community richness and indirect positive effects due to losses of natural enemies. Plant community changes with habitat fragmentation added to the indirect effects but with little predictable impact. Here, we evaluated habitat fragmentation effects on both herbivory and herbivore diversity, using novel hierarchical meta‐analyses. Across 89 studies, we found a negative effect of habitat fragmentation on abundance and species richness of herbivores, but only a non‐significant trend on herbivory. Reduced area and increased isolation of remaining fragments yielded the strongest effect on abundance and species richness, while specialist herbivores were the most vulnerable to habitat fragmentation. These fragmentation effects were more pronounced in studies with large spatial extent. The strong reduction in herbivore diversity, but not herbivory, indicates how important common generalist species can be in maintaining herbivory as a major ecosystem process.     

Effects of a short fire‐return interval on resources and assemblage structure of birds in a tropical savanna

Fire frequency is a key land management issue, particularly in tropical savannas where fire is widely used and fire recurrence times are often short. We used an extended Before‐After‐Control‐Impact design to examine the impacts of repeated wet‐season burning for weed control on bird assemblages in a tropical savanna in north Queensland, Australia. Experimentally replicated fire treatments (unburnt, singularly bunt, twice burnt), in two habitats (riparian and adjacent open woodland), were surveyed over 3 years (1 year before the second burn, 1 year post the second burn, 2 years post the second burn) to examine responses of birds to a rapid recurrence of fire. Following the second burn, species richness and overall bird abundance were lower in the twice‐burnt sites than either the unburnt or singularly burnt sites. Feeding group composition varied across year of survey, but within each year, feeding guilds grouped according to fire treatment. In particular, abundance of frugivores and insectivores was lower in twice‐burnt sites, probably because of the decline of a native shrub that produces fleshy fruits, Carissa ovata. Although broader climatic variability may ultimately determine overall bird assemblages, our results show that a short fire‐return interval will substantially influence bird responses at a local scale. Considering that fire is frequently used as a land management tool, our results emphasize the importance of determining appropriate fire‐free intervals.     

Responses of Phytoplankton to in‐situ Nutrient Enrichment; Potential Influences on Species Dominance in a River

The Hawkesbury River at Sackville, New South Wales, Australia is fresh and vigorously mixed by tidal movement. The location has frequent blooms of Microcystis aeruginosa, which have been recorded occurring throughout the year, including winter temperatures as low as 13 °C. Nutrient enrichment tests were performed in‐situ on the natural phytoplankton population in 1997 and 1998 while Microcystis aeruginosa dominated (covering both summer and winter periods). These experiments compared population changes under the ambient nutrient regime with those after additions of ortho‐phosphate, nitrate, ammonia and various combinations of these nutrients. Under ambient conditions, the Microcystis population was able to grow significantly (P < 0.05) while most non‐cyanobacterial phytoplankton did not. Nutrient additions induced a variety of nutrient limitation responses that often varied between genera of major groups i.e. in the Chlorophyceae (Actinastrum sp. responded to phosphorus while Psephonema sp. responded to nitrogen). The possibility that shifts in population dominance from Chlorophyceae to the Cyanobacteria (M. aeruginosa) at Sackville are in response to competition for limiting nutrients is discussed.     

Vocal Mimicry of Alarm‐Associated Sounds by a Drongo Elicits Flee and Mobbing Responses from Other Species that Participate in Mixed‐Species Bird Flocks

A growing number of studies have shown that vocal mimicry appears to be adaptive for some bird species, although the exact function of this behaviour varies among species. Previous work has looked at the function of the vocal mimicry of non‐alarm sounds by the Greater Racket‐tailed Drongo (Dicurus paradiseus). But drongos also imitate sounds associated with danger, such as predators' vocalisations or the mobbing‐specific vocalisations of other prey species, raising the question of whether the function of mimicry can vary even within a species. In a playback experiment, we compared the effect on other species of different drongo vocalisations including: (1) predator mimicry, (2) mobbing mimicry, (3) drongo species‐specific alarms, (4) drongo species‐specific non‐alarms and (5) a control (barbet) sound. Both mobbing mimicry and drongo species‐specific alarms elicited flee responses from the most numerous species in the flocks, the Orange‐billed Babbler (Turdoides rufescens). Mobbing mimicry also elicited mobbing responses from the Orange‐billed Babbler and from another gregarious babbler, the Ashy‐headed Laughingthrush (Garrulax cinereifrons); when responses from both species were considered together, they were elicited at a significantly higher level by mobbing mimicry than by the barbet control, and a level that tended to be higher (0.07 < p < 0.10) than the response to drongo‐specific alarms. Predator mimicry elicited flee and mobbing responses at an intermediary level. Our results support the hypotheses that mobbing mimicry is a specific category of mimicry that helps attract the aid of heterospecifics during mobbing and that alarm mimicry can in some cases be beneficial to the caller.     

Long‐term after‐effects of cold exposure in adult Alphitobius diaperinus (Tenebrionidae): the need to link survival ability with subsequent reproductive success

1. The effects of cold acclimation and cold exposure on the survival and reproductive capabilities of Alphitobius diaperinus (Tenebrionidae) adult beetles are examined. 2. First, the impact of temperature on survival duration was assessed by placing beetles in a range of cool temperature treatments. Second, the importance of acclimation duration was assessed. Third, the impact of thermal stress on subsequent reproductive ability was examined for beetles that had no previous cold exposure, and for beetles that had been subjected to previous cold exposure (i.e. acclimated) at various conditions, including fluctuating temperatures. 3. In all groups, the number of recorded survivors was strongly impacted by recovery period duration (i.e. 2 vs. 10 days). Survival of non‐acclimated and 3‐day acclimated beetles, expressed as lethal time for 50% of the samples, was reduced significantly when the insects were re‐assessed for survival at 10 days after being returned to optimal growth conditions (7.9 ± 0.4 vs. 5.1 ± 0.6 days and 8.8 ± 0.5 vs. 6.8 ± 0.6 days, respectively). 4. Insects that had been subject to cold acclimation expressed better subsequent reproduction success than non‐acclimated beetles. This beneficial impact increased when the acclimation period was prolonged, but some longer acclimation periods had no significant impact on survival. 5. Our results indicate that cold exposure has the capacity to irreversibly damage the reproductive system and that insect survival depends on the duration of the recovery period. Both the survival ability and subsequent reproductive output have to be examined to objectively determine insect cold resistance.     

Assisted reproduction and justice: Threats to a new model in a low‐ and middle‐income country

Infertility is an unpredictable but widespread condition. While high‐income countries grapple with when, or how to cover the costs of assisted reproductive technology (ART), such as in‐vitro fertilisation (IVF), these services are generally only available to wealthy persons at private facilities in low‐ and middle‐income countries (LMICs). Although the principle of non‐interference with normal individual reproductive rights is robust, whether it is also the responsibility of collective society to provide the means (when ART applies) to achieve pregnancy, is controversial. Recently, a low‐cost model was developed at a South African public institution. The target population for this model was “helpless and marginalised, childless couples”, but a new threat has arisen, namely, infertile couples who could conceivably afford private care. In the allocation of this scarce resource, we argue for a prioritarian response that first addresses the worst‐off, in order to even out unequal access imposed by sharp differences in income.