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Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice 总被引:3,自引:0,他引:3
MIGUEL A. PIÑEROS ZHIYE WANG WENXIA WANG CHANGYING LI ZHONGCHANG WU LEON V. KOCHIAN PING WU 《Plant, cell & environment》2014,37(5):1159-1170
We characterized the function of two rice phosphate (Pi) transporters: OsPHT1;9 (OsPT9) and OsPHT1;10 (OsPT10). OsPT9 and OsPT10 were expressed in the root epidermis, root hairs and lateral roots, with their expression being specifically induced by Pi starvation. In leaves, expression of the two genes was observed in both mesophyll and vasculature. High‐affinity Km values for Pi transport of OsPT9 and OsPT10 were determined by yeast experiments and two‐electrode voltage clamp analysis of anion transport in Xenopus oocytes expressing OsPT9 and OsPT10. Pi uptake and Pi concentrations in transgenic plants harbouring overexpressed OsPT9 and OsPT10 were determined by Pi concentration analysis and 33P‐labelled Pi uptake rate analysis. Significantly higher Pi uptake rates in transgenic plants compared with wild‐type plants were observed under both high‐Pi and low‐Pi solution culture conditions. Conversely, although no alterations in Pi concentration were found in OsPT9 or OsPT10 knockdown plants, a significant reduction in Pi concentration in both shoots and roots was observed in double‐knockdown plants grown under both high‐ and low‐Pi conditions. Taken together, our results suggest that OsPT9 and OsPT10 redundantly function in Pi uptake. 相似文献
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Overexpression of the IbMYB1 gene in an orange‐fleshed sweet potato cultivar produces a dual‐pigmented transgenic sweet potato with improved antioxidant activity 下载免费PDF全文
Sung‐Chul Park Yun‐Hee Kim Sun Ha Kim Yu Jeong Jeong Cha Young Kim Joon Seol Lee Ji‐Yeong Bae Mi‐Jeong Ahn Jae Cheol Jeong Haeng‐Soon Lee Sang‐Soo Kwak 《Physiologia plantarum》2015,153(4):525-537
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Zhi‐Sheng Xu Qing‐Qing Yang Kai Feng Xiao Yu Ai‐Sheng Xiong 《Plant biotechnology journal》2020,18(7):1585-1597
Purple carrots, the original domesticated carrots, accumulate highly glycosylated and acylated anthocyanins in root and/or petiole. Previously, a quantitative trait locus (QTL) for root‐specific anthocyanin pigmentation was genetically mapped to chromosome 3 of carrot. In this study, an R2R3‐MYB gene, namely DcMYB113, was identified within this QTL region. DcMYB113 expressed in the root of ‘Purple haze’, a carrot cultivar with purple root and nonpurple petiole, but not in the roots of two carrot cultivars with a purple root and petiole (Deep purple and Cosmic purple) and orange carrot ‘Kurodagosun’, which appeared to be caused by variation in the promoter region. The function of DcMYB113 from ‘Purple haze’ was verified by transformation in ‘Cosmic purple’ and ‘Kurodagosun’, resulting in anthocyanin biosynthesis. Transgenic ‘Kurodagosun’ carrying DcMYB113 driven by the CaMV 35S promoter had a purple root and petiole, while transgenic ‘Kurodagosun’ expressing DcMYB113 driven by its own promoter had a purple root and nonpurple petiole, suggesting that root‐specific expression of DcMYB113 was determined by its promoter. DcMYB113 could activate the expression of DcbHLH3 and structural genes related to anthocyanin biosynthesis. DcUCGXT1 and DcSAT1, which were confirmed to be responsible for anthocyanins glycosylation and acylation, respectively, were also activated by DcMYB113. The WGCNA identified several genes co‐expressed with anthocyanin biosynthesis and the results indicated that DcMYB113 may regulate anthocyanin transport. Our findings provide insight into the molecular mechanism underlying root‐specific anthocyanin biosynthesis and further modification in carrot and even other root crops. 相似文献
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Yasuhiro Shimizu Kazuhiro Maeda Mika Kato Koichiro Shimomura 《In vitro cellular & developmental biology. Plant》2010,46(5):460-465
Gynura bicolor DC., a traditional vegetable in Japan, is cultivated as Kinjisou and Suizenjina in Ishikawa and Kumamoto prefectures, respectively.
The adaxial side of the leaves of G. bicolor grown in a field is green, and the abaxial side is reddish purple. It has been reported that these reddish purple pigments
are anthocyanins. Although we established a culture system of G. bicolor, the leaves of G. bicolor plants grown under our culture conditions showed green color on both sides of all leaves. We investigated the effects of
phytohormones and chemical treatments on anthocyanin accumulation in cultured plants. Although anthocyanin accumulation in
the leaves was slightly stimulated, anthocyanins accumulation in the roots of the cultured plant was induced remarkably by
25–50 μM methyl jasmonate (MJ) treatment. This induction was affected by light irradiation and sucrose concentration in the
culture medium. However, salicylic acid (SA) and 1-aminocyclopropane-1-carboxylic acid did not induce anthocyanin accumulation
in roots. And then, combinations of MJ and SA or MJ and AgNO3 did not stimulate the anthocyanin accumulation in the root as found in the case of treatment by MJ solely. 相似文献
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Brendon Conlan Rosemary Birch Celine Kelso Sophie Holland Amanda P. De Souza Stephen P. Long Jennifer L. Beck Spencer M. Whitney 《Plant, cell & environment》2019,42(4):1287-1301
The folding and assembly of Rubisco large and small subunits into L8S8 holoenzyme in chloroplasts involves many auxiliary factors, including the chaperone BSD2. Here we identify apparent intermediary Rubisco‐BSD2 assembly complexes in the model C3 plant tobacco. We show BSD2 and Rubisco content decrease in tandem with leaf age with approximately half of the BSD2 in young leaves (~70 nmol BSD2 protomer.m2) stably integrated in putative intermediary Rubisco complexes that account for <0.2% of the L8S8 pool. RNAi‐silencing BSD2 production in transplastomic tobacco producing bacterial L2 Rubisco had no effect on leaf photosynthesis, cell ultrastructure, or plant growth. Genetic crossing the same RNAi‐bsd2 alleles into wild‐type tobacco however impaired L8S8 Rubisco production and plant growth, indicating the only critical function of BSD2 is in Rubisco biogenesis. Agrobacterium mediated transient expression of tobacco, Arabidopsis, or maize BSD2 reinstated Rubisco biogenesis in BSD2‐silenced tobacco. Overexpressing BSD2 in tobacco chloroplasts however did not alter Rubisco content, activation status, leaf photosynthesis rate, or plant growth in the field or in the glasshouse at 20°C or 35°C. Our findings indicate BSD2 functions exclusively in Rubisco biogenesis, can efficiently facilitate heterologous plant Rubisco assembly, and is produced in amounts nonlimiting to tobacco growth. 相似文献
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Characterisation of flower colouration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits 下载免费PDF全文
H. Du L. Lai F. Wang W. Sun L. Zhang X. Li L. Wang L. Jiang Y. Zheng 《Plant biology (Stuttgart, Germany)》2018,20(1):121-129
- Floral colour is a key reproductive character, often associated with environmental adaptation, and subject to human intervention. A large number of Rhododendron species differ widely in flower colour, providing a good model for flower colouration. The chromatic features and anthocyanin compositions of 30 species from seven subgenera were systematically analysed.
- The Royal Horticultural Society Colour Chart and CIE L*a*b* system were employed to describe and investigate flower colours. The UPLC‐PDA/ESI‐MSn system was used to identify and quantify anthocyanins in petal extracts.
- The flower colours of 30 Rhododendron species were categorised into four groups – red, purplish pink, purple and white. Seven anthocyanins were identified and quantified in petals: delphinidin, cyanidin and malvidin 3‐O‐arabinoside‐5‐O‐glucosides, cyanidin 3,5‐di‐O‐glucoside, 3‐O‐galactoside and 3‐O‐arabinoside, and delphinidin 3‐O‐glucoside. The red‐flowered species mainly contained cyanidin monoglycosides and had much higher total anthocyanin content than purplish pink‐ and purple‐flowered species. Purplish pink‐ and purple‐flowered species had similar anthocyanin types and content. The chromatic differences were significant among groups, except the purplish pink and purple groups. Statistical analysis showed that Cy3Gal and Cy3Arb are characteristic for red‐flowered species, and Mv3Arb5G and Dp3Arb5G play important roles in purple colouration; their contents were major components that greatly affected the chromatic parameters. In total, 21 flavonol derivates were identified. However, total flavonol content and co‐pigmentation index showed no significant difference or correlation among/with colour groups, suggesting that flavonols might not play a major role in colouration.
- These results enhance our knowledge of the biochemical basis of flower colouration in Rhododendron species, and provide a foundation for genetic variation studies and aid in breeding cultivars with novel flower colours.
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Map‐based cloning of the pear gene MYB114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis 下载免费PDF全文
Gaifang Yao Meiling Ming Andrew C. Allan Chao Gu Leiting Li Xiao Wu Runze Wang Yaojun Chang Kaijie Qi Shaoling Zhang Jun Wu 《The Plant journal : for cell and molecular biology》2017,92(3):437-451
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S. G. Spivak I. N. Berdichevets D. G. Yarmolinsky T. V. Maneshina G. V. Shpakovski N. A. Kartel 《Russian Journal of Genetics》2009,45(9):1067-1073
In steroidogenic animal tissues cytochrome P450SCC catalizes the conversion of cholesterol into pregnenolone, a common metabolic precursor of all steroid hormones. To study
the possibility of functioning of mammalian cytochrome P450SCC in plants and the mechanism of its integration in the plant steroidogenic system, transgenic plants of tobacco Nicotiana tabacum L. were developed carrying cDNA of CYP11A1 encoding cytochrome P450SCC of bovine adrenal cortex. Pregnenolone, a product of the reaction catalyzed by cytochrome P450SCC, was discovered in the steroid-containing fraction of transgenic plants. Transgenic plants are characterized by a reduced
period of vegetative development (early flowering and maturation of bolls) and increased productivity. The contents of soluble
protein and carbohydrates in leaves and seeds of transgenic plants are essentially higher than the contents of these components
in leaves and seeds of control plants. 相似文献