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1.
Tsukazaki H Yamashita K Yaguchi S Yamashita K Hagihara T Shigyo M Kojima A Wako T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):501-510
To determine the chromosomal location of bunching onion (Allium fistulosum L.) simple sequence repeats (SSRs) and bulb onion (A. cepa L.) expressed sequence tags (ESTs), we used a complete set of bunching onion–shallot monosomic addition lines and allotriploid
bunching onion single alien deletion lines as testers. Of a total of 2,159 markers (1,198 bunching onion SSRs, 324 bulb onion
EST–SSRs and 637 bulb onion EST-derived non-SSRs), chromosomal locations were identified for 406 markers in A. fistulosum and/or A. cepa. Most of the bunching onion SSRs with identified chromosomal locations showed polymorphism in bunching onion (89.5%) as well
as bulb onion lines (66.1%). Using these markers, we constructed a bunching onion linkage map (1,261 cM), which consisted
of 16 linkage groups with 228 markers, 106 of which were newly located. All linkage groups of this map were assigned to the
eight basal Allium chromosomes. In this study, we assigned 513 markers to the eight chromosomes of A. fistulosum and A. cepa. Together with 254 markers previously located on a separate bunching onion map, we have identified chromosomal locations
for 766 markers in total. These chromosome-specific markers will be useful for the intensive mapping of desirable genes or
QTLs for agricultural traits, and to obtain DNA markers linked to these. 相似文献
2.
A genetic map of an interspecific cross in Allium based on amplified fragment length polymorphism (AFLPTM) markers 总被引:1,自引:0,他引:1
A.W. van Heusden J.W. van Ooijen R. Vrielink-van Ginkel W. H. J. Verbeek W. A. Wietsma C. Kik 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):118-126
Segregation of 692 polymorphic AFLPTM (amplified fragment length polymorphism) fragments was determined in an F2 of the interspecific cross A. roylei x A. cepa. Two different enzyme combinations were used, PstI/MseIand EcoRI/MseI; in the latter one extra selective nucleotide was added to the MseI primer. The map based on A. cepa markers consisted of eight linkage groups with 262 markers covering 694 cM of the expected 800 cM. The map based on A. roylei markers comprised 15 linkage groups with 243 markers and had a length of 626 cM. The two maps were not integrated, and 25%
of the markers remained unlinked. One of the alliinase genes and a SCAR marker linked to the disease resistance gene to downy
mildew are present on this map. Of the AFLP markers, 50—80% were polymorphic between A. cepa and A. roylei; the level of polymorphic markers between different A. cepa accessions was4-8%.
Received: 28 August 1998 / Accepted: 31 March 1999 相似文献
3.
Tsukazaki H Yamashita K Yaguchi S Masuzaki S Fukuoka H Yonemaru J Kanamori H Kono I Hang TT Shigyo M Kojima A Wako T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(8):1213-1223
We have constructed a linkage map of bunching onion (Allium fistulosum L., 2n = 16) using an F(2) population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa. 相似文献
4.
Monosomic alien addition lines (MAALs) are useful for assigning linkage groups to chromosomes. We examined whether the chromosomal rearrangements following the introduction of a single onion (Allium cepa) chromosome into the Allium fistulosum genome were produced by homeologous crossing over or by a nonreciprocal conversion event. Among the monosomic lines available, 17 were studied by fluorescent genomic in situ hybridisation, using total A. cepa genomic DNA as the probe and total A. fistulosum genomic DNA as the competitor. In this way, rearrangements such as chromosomal translocations between A. cepa and A. fistulosum were identified as terminal regions consisting of tandem DNA repeats. Homeologous crossing over between the two closely related genomes occurred in 4 of the 17 lines, suggesting that such events are not rare. On the basis of a detailed molecular cytogenetic characterisation, we identified true monosomic alien addition lines for A. cepa chromosomes 3, 4, 5, 7, and 8 that can reliably be used in genetic studies. 相似文献
5.
Selfed progeny of a complete set of Allium fistulosum - Allium cepa monosomic addition lines (2n = 2x + 1 = 17, FF+1A-FF+8A) were produced to examine the transmission rates of respective alien chromosomes. All eight types of the selfed monosomic additions set germinable seeds. The numbers of chromosomes (2n) in the seedlings were 16, 17, or 18. The eight extra chromosomes varied in transmission rate (%) from 9 (FF+2A) to 49 (FF+8A). The complete set of monosomic additions was reproduced successfully by self-pollination. A reliable way to maintain a set of Allium monosomic additions was developed using a combination of two crossing methods, selfing and female transmission. FF+8A produced two seedlings with 18 chromosomes. Cytogenetical analyses, including GISH, showed that the seedlings were disomic addition plants carrying two entire homologous chromosomes from A. cepa in an integral diploid background of A. fistulosum. Flow cytometry analysis showed that a double dose of the alien 8A chromosome caused fluorescence intensity values spurring in DNA content, and isozyme analysis showed increased glutamate dehydrogenase activity at the gene locus Gdh-1. 相似文献
6.
Vu HQ Yoshimatsu Y Khrustaleva LI Yamauchi N Shigyo M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(7):1241-1257
To produce alien monosomic addition lines (AMALs) of Allium cepa (genomes CC, 2n = 2x = 16) carrying extrachromosomes from Allium roylei (RR, 2n = 2x = 16), reciprocal backcrossing of allotriploids (2n = 24, CCR) with diploids (2n = 16, CC) and selfing of a single allotriploid were carried out. The chromosome numbers in the BC2F1 and BC1F2 progenies ranged from 16 to 32. Forty-eight plants were recorded to possess 2n = 17 among a total of 169 plants in observation. Through the analyses of isozymes, expressed sequence tag (EST) markers,
and karyotypes, all eight possible types of A. cepa–A. roylei monosomic addition lines (CC+1R–CC+8R) could be identified. Seven types of representative AMALs (without CC+2R) were used
for the GISH analysis of somatic chromosomes. Except for CC+6R, all AMALs showed an entire (unrecombined) extrachromosome
from A. roylei in the integral diploid background of A. cepa. A single recombination between A. cepa and A. roylei was observed on the extrachromosome in the remaining type. All alloplasmic AMALs possessing A. roylei cytoplasm showed high or complete pollen sterility. Only the autoplasmic CC+4R with A. cepa cytoplasm possessed relatively high pollen fertility. The bulbs of CC+4R displayed the distinct ovoid shape that discriminates
them from spherical or oval ones in other AMALs. Downy mildew screening in the field showed higher resistance in A. roylei, a hypo-allotriploid (CCR-nR, 2n = 23), and an allotriploid (CCR, 2n = 24). Meanwhile, no complete resistance was found in some AMALs examined. This was the first trial toward the establishment
of a complete set of A. cepa–A. roylei monosomic additions. 相似文献
7.
AFLP and CAPS linkage maps of Cryptomeria japonica 总被引:7,自引:0,他引:7
A. M. Nikaido T. Ujino H. Iwata K. Yoshimura H. Yoshimura Y. Suyama M. Murai K. Nagasaka Y. Tsumura 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(6):825-831
We have used two DNA marker systems, AFLP and CAPS, in a two-way pseudo-testcross strategy applied to an F1 population to construct genetic linkage maps of two local sugi cultivars. The AFLP markers detected about eight polymorphisms
per parent per primer combination. Using 38 primer combinations, 612 AFLPs were detected in ’Haara 4’ and ’Kumotooshi’, of
which 305 segregated in a 1:1 ratio (P>0.05). A total of 91 markers (83 AFLP and 8 CAPS) in ’Haara 4’ and 132 (123 AFLP and 9 CAPS) in ’Kumotooshi’ were distributed
among 19 and 23 linkage groups, respectively, each of which included 2–17 markers. Maps of ’Haara 4’ and ’Kumotooshi’ spanned
1266.1 cM and 1992.3 cM, and covered approximately 50% and 80% of the sugi genome, respectively. Sequences derived from cDNA,
which were previously used to construct a sugi linkage map, were also placed on our linkage maps as CAPS markers. Where a
’two-way pseudo-testcross’ is used, more than half of the sugi CAPS developed can be used to construct linkage maps for each
parental family. The saturation of mapped markers, and the integration of several linkage maps derived from different mapping
populations, is anticipated in the near future.
Received: 15 August 1999 / Accepted: 27 August 1999 相似文献
8.
S. L. Fox E. N. Jellen S. F. Kianian H. W. Rines R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):320-326
The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage
groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population.
Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes.
In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the
same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally,
the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences
among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome.
Received: 24 April 2000 / Accepted: 10 May 2000 相似文献
9.
L. I. Khrustaleva C. Kik 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):17-26
Introgression of Allium fistulosum into the genome of A. cepa using A. roylei as a bridging species was studied by means of genomic in situ hybridization (GISH). Here we demonstrate for the first time that A. fistulosum can be stably introgressed into A. cepa with a bridge-cross. The first and second bridge-cross generations were fertile, although pollen was sterile in some individuals.
Only occasionally were there translocations in the second generation bridge-cross. Recombination between the three genomes
was frequently seen in meiotic anaphase 1 and prophase 2 chromosomes of the first generation bridge cross and in mitotic chromosomes
of the second generation bridge-cross. The number of observed recombination points in anaphase 1 and prophase 2 significantly
exceeded the value expected from chiasma frequency in metaphase 1. Recombination points were randomly distributed, thus the
A. cepa or A. roylei type of random distribution prevails over the A. fistulosum type of proximally localised chiasmata.
Received: 15 December 1998 / Accepted: 18 February 1999 相似文献
10.
Yaguchi S McCallum J Shaw M Pither-Joyce M Onodera S Shiomi N Yamauchi N Shigyo M 《Plant & cell physiology》2008,49(5):730-739
Onion and shallot (Allium cepa L.) exhibit wide variation in bulb fructan content, and the Frc locus on chromosome 8 conditions much of this variation. To understand the biochemical basis of Frc, we conducted biochemical and genetic analyses of Allium fistulosum (FF)-shallot (A. cepa Aggregatum group) alien monosomic addition lines (AALs; FF+1A-FF+8A) and onion mapping populations. Sucrose and fructan levels in leaves of FF+2A were significantly lower than in FF throughout the year, and the springtime activity of acid invertase was also lower. FF+8A showed significantly higher winter sucrose accumulation and sucrose phosphate synthase (SPS) activity. Inbred high fructan (Frc_) lines from the 'W202Ax Texas Grano 438' onion population exhibited significantly higher sucrose levels prior to bulbing than low fructan (frcfrc) lines. Sucrose synthase (SuSy) activity in these lines was correlated with leaf hexose content but not with Frc phenotype. Markers for additional candidate genes for sucrose metabolism were obtained by cloning a major SPS expressed in onion leaf and exhaustively mining onion expressed sequence tag resources. SPS and SuSy loci were assigned to chromosome 8 and 6, respectively, using AALs and linkage mapping. Further loci were assigned, using AALs, to chromosomes 1 (sucrose phosphate phosphatase), 2 (SuSy and three invertases) and 8 (neutral invertase). The concordance between chromosome 8 localization of SPS and elevated leaf sucrose levels conditioned by high fructan alleles at the Frc locus in bulb onion or alien monosomic additions of chromosome 8 in A. fistulosum suggest that the Frc locus may condition variation in SPS activity. 相似文献
11.
Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping 总被引:11,自引:0,他引:11
S. Nandi P. K. Subudhi D. Senadhira N. L. Manigbas S. Sen-Mandi N. Huang 《Molecular & general genetics : MGG》1997,255(1):1-8
By combining the amplified fragment length polymorphism (AFLP) technique with selective genotyping, we constructed a linkage
map for rice and assigned each linkage group to a corresponding chromosome. The AFLP map, consisting of 202 AFLP markers,
was generated from 74 recombinant inbred lines (RIL) which were selected from both extremes of the population (250 lines)
with respect to the response to complete submergence. Map length was 1756 cM, with an average interval size of 8.5 cM. To
assign linkage groups to chromosomes, we used 50 previously mapped AFLP markers as anchor markers distributed over the 12
chromosomes. Other AFLP markers were then assigned to specific chromosomes based on their linkage to anchor markers. This
AFLP map is equivalent to the RFLP/AFLP map constructed previously as the anchors were in the same order in both maps. Furthermore,
tests with two restriction fragment length polymorphism (RFLP) markers and two sequence-tagged site (STS) markers showed that
they mapped in the expected positions. Using this AFLP map, a major gene for submergence tolerance was localized on chromosome
9. Quantitative trait loci (QTL) associated with submergence tolerance were detected on chromosomes 6, 7, 11, and 12. We conclude
that the combination of AFLP mapping and selective genotyping provides a much faster and easier approach to QTL identification
than the use of RFLP markers.
Received: 20 December 1996 / Accepted: 21 January 1997 相似文献
12.
A linkage map of garden pea was constructed on the basis of 114 plants (F2 generation) derived from a cross combination Wt10245 x Wt11238. The map, consisting of 204 morphological, isozyme, AFLP, ISSR, STS, CAPS and RAPD markers, was used for interval mapping of quantitative trait loci (QTLs) controlling seed number, pod number, 1000-seed weight, 1000-yield, and seed protein content. Characterization of each QTL included identification of QTL position with reference to the flanking markers, estimation of the part of variance explained by this QTL, and determination of its gene action. The yield-related traits were measured in F2 plants and in F4 recombinant inbred lines (RILs). The interval mapping revealed two to six QTLs per trait, demonstrating linkage to seven pea chromosomes. A total of 37 detected QTLs accounted for 9.1-55.9% of the trait's phenotypic variation and showed different types of gene action. As many as eight and ten QTLs influencing the analysed traits were mapped in linkage groups III and V, respectively, indicating an important role of these regions of the pea genome in the control of yield and seed protein content. 相似文献
13.
A. M. Chèvre F. Eber P. Barret P. Dupuy J. Brace 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):603-611
The F1 hybrids produced after crosses between B. gra and B. oleracea were backcrossed two or three times to B. oleracea. Among the 14 plants analysed, five were monosomic addition lines (2n=19), six were double monosomic addition lines (2n=20)
and three had three or four additional chromosomes. From these lines, 14 isozyme and 80 RAPD loci were localized on the eight
chromosomes of B. nigra. The comparison between B. napus-B. nigra, from which five B. nigra chromosomes were already described, and the new set of B. oleracea-B. nigra addition lines was performed using five isozyme and 22 common RAPD loci. The homology of the common RAPD loci was confirmed
by hybridization of the two sets of addition lines as well as the presence of duplicated loci on different chromosomes. For
the five added chromosomes available on the two genetic backgrounds, i.e. B. napus and B. oleracea, using isozyme markers, the chromosome transmission rate was studied from backcross progeny using the recurrent parent either
as male or as female and from the selfing of monosomic addition lines. For each chromosome, no difference was detected between
male and female transmission except for chromosome 3. This latter presented a percentage of female transmission of around
20%, close to the ones observed for the other chromosomes, but a very low male transmission (1.3%). The analysis from restriction
enzyme digests of PCR products, obtained from primers selected in highly conserved regions of self-incompatible genes, suggested
that the chromosome 3 probably carried the SLG-B. nigra locus.
Received: 25 September 1996 / Accepted: 18 October 1996 相似文献
14.
15.
First and second backcrosses of amphidiploid hybrids (2n = 4x = 32, genomes AAFF) between shallot (Allium cepa Aggregatum group) and A. fistulosum were conducted to produce A. cepa - A. fistulosum alien addition lines. When shallot (A. cepa Aggregatum group) was used as a pollinator, the amphidiploids and allotriploids set germinable BC(1) and BC(2) seeds, respectively. The 237 BC(1) plants mainly consisted of 170 allotriploids (2n = 3x = 24, AAF) and 42 hypo-allotriploids possessing 23 chromosomes, i.e., single-alien deletions (2n = 3x-1 = 23, AAF-nF). The single-alien deletions in the BC(1) progeny showed dwarfing characteristics and were discriminated from the allotriploids (2n = 24) and hyper-allotriploids (2n = 25) by means of flow cytometric analysis. The chromosome numbers of 46 BC(2) seedlings varied from 16 to 24. Eight monosomic additions (2n = 2x+1 = 17, AA+nF) and 20 single-alien deletions were found in these BC(2) seedlings. Consequently, six kinds of A. cepa - A. fistulosum alien chromosome additions possessing different chromosome numbers (2n = 17, 18, 20, 21, 22, 23) were recognized in the BC(1) and BC(2) populations. A total of 79 aneuploids, including 62 single-alien deletions, were analyzed by a chromosome 6F-specific isozyme marker (Got-2) in order to recognize its existence in their chromosome complements. This analysis revealed that two out of 62 single-alien deletions did not possess 6F. One (AAF-6F) out of the possible eight single-alien deletions could be identified at first. The present study is a first step toward the development of a useful tool, such as a complete set of eight different single-alien deletions, for the rapid chromosomal assignment of genes and genetic markers in A. fistulosum. 相似文献
16.
L. I. Khrustaleva C. Kik 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):8-14
Allium fistulosum harbours a number of desirable agronomical traits for the breeding of onions. However exploitation of A. fistulosum for onion breeding via direct sexual hybridization is problematic. Therefore, we examined if a bridge cross, using A. roylei as a bridging species, might provide an alternative. By means of genomic in situ hybridization (GISH) we showed that each
of the three parental genomes can be distinguished from the others in interspecific hybrids, suggesting that these genomes
contain sufficiently different repetitive DNA families. We succeeded in carrying out multi-colour GISH to metaphase spreads
of a first-generation bridge-cross individual [A. cepa× (A. fistulosum×A. roylei], which is composed of three parental genomes. Recombination between the genomes of A. fistulosum and A. roylei took place to a large extent: 7 recombined chromosomes were observed, and it could be shown that the proximal regions of
the recombined A. fistulosum/A. roylei chromosomes belonged to the former, whereas the distal parts belonged to the latter. The high percentage of bound bivalent
arms in metaphase I of pollen mother cells of a fertile bridge-cross individual suggests the introgression of A. fistulosum genes, mediated by A. roylei, into the genome of A. cepa. However, the presence of univalents reflects decreased pairing and recombination between the three genomes. Pollen fertility
and pollen-tube growth of the first-generation bridge-cross individual seem to be sufficient to produce a second generation
bridge-cross (A. cepa×first-generation bridge cross) progeny.
Received: 27 May 1997 / Accepted: 30 June 1997 相似文献
17.
Development and mapping of AFLP markers linked to the sorghum fertility restorer gene <Emphasis Type="Italic">rf4</Emphasis> 总被引:2,自引:2,他引:0
Wen L Tang HV Chen W Chang R Pring DR Klein PE Childs KL Klein RR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):577-585
The restoration of male fertility in the sorghum IS1112 C (A3) male-sterile cytoplasm is through a two-gene gametophytic system
involving complementary action of the restoring alleles Rf3 and Rf4. To develop markers suitable for mapping rf4, AFLP technology was applied to bulks of sterile and fertile individuals from a segregating BC3F1 population. Three AFLP markers linked to rf4 were identified and subsequently converted to STS/CAPS markers, two of which are co-dominant. Based on a population of 378
BC1F1 individuals, two STS/CAPS markers, LW7 and LW8, mapped to within 5.31 and 3.18 cM, respectively, of rf4, while an STS marker, LW9, was positioned 0.79 cM on the flanking side of rf4. Markers LW8 and LW9 were used to screen sorghum BAC libraries to identify the genomic region encoding rf4. A series of BAC clones shown to represent a genomic region of linkage group E were identified by the rf4-linked markers. A contig of BAC clones flanking the LW9 marker represent seed clones on linkage group E, from which fine
mapping of the rf4 locus and chromosome walking can be initiated.
Received: 20 June 2001 / Accepted: 3 August 2001 相似文献
18.
Sabharwal V Negi MS Banga SS Lakshmikumaran M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(1):160-166
Association mapping of the seed-coat colour with amplified fragment length polymorphism (AFLP) markers was carried out in 39 Brassica juncea lines. The lines had genetically diverse parentages and varied for seed-coat colour and other morphological characters. Eleven AFLP primer combinations were used to screen the 39 B. juncea lines, and a total of 335 polymorphic bands were detected. The bands were analysed for association with seed-coat colour using multiple regression analysis. This analysis revealed 15 markers associated with seed-coat colour, obtained with eight AFLP primer combinations. The marker E-ACA/M-CTG350 explained 69% of the variation in seed-coat colour. This marker along with markers E-AAC/M-CTC235 and E-AAC/M-CTA250 explained 89% of the total variation. The 15 associated markers were validated for linkage with the seed-coat colour loci using a recombinant inbred line (RIL) mapping population. Bands were amplified with the eight AFLP primer combinations in 54 RIL progenies. Of the 15 associated markers, 11 mapped on two linkage groups. Eight markers were placed on linkage group 1 at a marker density of 6.0 cM, while the remaining three were mapped on linkage group 2 at a marker density of 3.6 cM. Marker E-ACA/M-CTG350 co-segregated with Gene1 controlling seed-coat colour; it was specific for yellow seed-coat colour and mapped to linkage group 1. Marker E-AAC/M-CTC235 (AFLP8), which had been studied previously, was present on linkage group 2; it was specific for brown seed-coat colour. Since AFLP markers are not adapted for large-scale applications in plant breeding, it is important to convert these to sequence-characterised amplified region (SCAR) markers. Marker E-AAC/M-CTC235 (AFLP8) had been previously converted into a SCAR. Work is in progress to convert the second of the linked markers, E-ACA/M-CTG350, to a SCAR. The two linked AFLP markers converted to SCARs will be useful for developing yellow-seeded B. juncea lines by means of marker-assisted selection.Communicated by H.F. Linskens 相似文献
19.
A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers
Jorge Dias Carlier Nelson Horta Sousa Tatiana Espírito Santo Geo Coppens d’Eeckenbrugge José Manuel Leit?o 《Molecular breeding : new strategies in plant improvement》2012,29(1):245-260
Despite the paramount importance of pineapple (Ananas comosus L.) in world production and trade of tropical fruits, the genomics of this crop is still lagging behind that of other tropical
fruit crops such as banana or papaya. A genetic map of pineapple was constructed using an F2 segregating population obtained
from a single selfed F1 plant of a cross A. comosus var. comosus (cv. Rondon, clone BR 50) × A. comosus var. bracteatus (Branco do mato, clone BR 20). Multiple randomly amplified markers (RAPD, ISSR and AFLP) were brought together with SSR and
EST-SSR markers identified among sequences uploaded to public databases and with sequence-specific markers (SCAR, SSR and
CAPS) derived from random amplified markers. Sixty-three randomly amplified markers (RAPD, ISSR and AFLP) were selected and
cloned, resulting in 71 sequences which were used to generate sequence-specific SCAR and CAPS markers. The present map includes
492 DNA markers: 57 RAPD, 22 ISSR, 348 AFLP, 20 SSR, 12 EST-SSR, 25 SCARs, 8 CAPS, and the morphological trait locus “piping”,
gathered into 33 linkage groups that integrate markers inherited from both botanical varieties, four linkage groups with markers
only from var. comosus and three linkage groups with markers exclusively from var. bracteatus. The relatively higher mapping efficiency of sequence-specific markers derived from randomly amplified markers (50.7%) versus
SSR (31.4%) and EST-SSR (28.9%) markers is discussed. Spanning over 80% of the 2,470 cM estimated average length of the genome,
the present map constitutes a useful research tool for molecular breeding and genomics projects in pineapple and other Bromeliaceae
species. 相似文献
20.
We used a complete set of Allium fistulosum - shallot (A. cepa Aggregatum group) monosomic addition lines (FF+1A - FF+8A) to identify shallot chromosomes affecting the production of sugars. In the alien addition lines grown over two years in an experimental field at Yamaguchi University (34 degrees N, 131 degrees E), shallot chromosomes 2A and 8A altered sugar contents in leaf-bunching onion (A. fistulosum). Except for FF+2A, every monosomic addition accumulated non-reducing sugars in winter leaf blades. FF+8A caused an increase in the amounts of non-reducing sugars in the winter. FF+2A hardly produced non-reducing sugar throughout the two-year study. These results indicated that genes related to non-reducing sugar metabolism are located on the 2A and 8A chromosomes. The results of regression analyses using 2002 data on A. fistulosum and the monosomic addition set revealed a correlation (r = 0.63 +/- 0.07; mean +/- SE., n = 9) between reducing sugar and monosaccharide (Glc+Fru) contents but no correlation between non-reducing sugar and sucrose contents. This result indicates the existence of other polysaccharides (e.g., scorodose) as non-reducing sugars in the leaf blade. 相似文献