The new biochemistry: blending the traditional with the other

Designing and presenting a modern biochemistry course is a challenging task. With an overabundance of topics to cover and a limited amount of class time, instructors must be creative and flexible. Traditionally, biochemical studies have been based on protein structure and function with emphasis on metabolism and bioenergetics. Developing methods in molecular biology are providing a new perspective on biochemistry. To train students for the future, it is imperative that these new topics be blended with the traditional. Model syllabi with this perspective are presented for a typical one-year, (28 weeks, two semester) course for biochemistry majors and the shorter survey course (one half-year, 14 weeks, one semester) for nonmajors, usually health professionals and biological science majors. The major change suggested from the present is to move the coverage of nucleic acid structure and function before metabolism. It is then possible to present metabolism in a more contemporary format with emphasis on regulation and integration.     

ProteoMod: A new tool to quantitate protein post-translational modifications

Post-translational modifications (PTMs) are known to regulate biological processes by controlling protein function. The effect of a PTM on protein function depends critically on the position and the number of modifications. While there are convenient methods available to qualitatively examine modifications like phosphorylation, glycosylation, acetylation and methylation, methods available for their quantitative assessment are cumbersome. We have developed a new tool that allows quantitation of the number of phosphorylation events in proteins with ease. The "ProteoMod" tool depends on shifts in the isoelectric points of proteins upon post-translational change. The extent of shift exhibited upon phosphorylation is algorithmically converted into the number of phosphorylations conferred. The validity of ProteoMod was confirmed by examining proteins with previously known number of phosphorylations. The list of proteins examined included HSP27, HSP70 and tumor suppressor p53. The approach can also be applied to estimate modifications like acetylation, methylation and sialylation in proteins. We analyzed shifts in isoelectric points due to sialylation events in N-glycoproteins. Using influenza hemagglutinin we show that shifts in isoelectric points correlate with intracellular distribution of this model membrane protein. In addition to extending the application of two dimensional gel electrophoresis to quantitate modifications, our study also highlights its potential use in cell biology.     

The shaping of Gujarati Cinema: Recognizing the new in traditional cultures

The study of ethnographic film has turned to the study of indigenous productions, primarily nonfictional videos made by native persons for internal and external consumption. This study was prompted by the need to locate alternate ways of seeing that Nonwestern peoples may have. This paper suggests that it is important to study local genius by studying their fictional films as well, and suggests that newness may not be found at first encounter but later after native groups domesticate foreign technology and make it their own tool. In citing this, the paper describes the various genres of films within contemporary Gujarati cinema and how the hybridity of its present forms has been procured from several sources that make for a distinctiveness which underlines emergent creative social forces at play.     

The intragenic approach as a new extension to traditional plant breeding

The novel intragenic approach to genetic engineering improves existing varieties by eliminating undesirable features and activating dormant traits. It transforms plants with native expression cassettes to fine-tune the activity and/or tissue specificity of target genes. Any intragenic modification of traits could, at least in theory, also be accomplished by traditional breeding and transgenic modification. However, the new approach is unique in avoiding the transfer of unknown or foreign DNA. By consequently eliminating various potential risk factors, this method represents a relatively safe approach to crop improvement. Therefore, we argue that intragenic crops should be cleared through the regulatory process in a timely and cost-effective manner.