共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
Krause K Maier RM Kofer W Krupinska K Herrmann RG 《Molecular & general genetics : MGG》2000,263(6):1022-1030
5.
6.
7.
8.
9.
10.
11.
Suzuki JY Ytterberg AJ Beardslee TA Allison LA Wijk KJ Maliga P 《The Plant journal : for cell and molecular biology》2004,40(1):164-172
We affinity-purified the tobacco plastid-encoded plastid RNA polymerase (PEP) complex by the alpha subunit containing a C-terminal 12 x histidine tag using heparin and Ni(2+) chromatography. The composition of the complex was determined by mass spectrometry after separating the proteins of the >900 kDa complex in blue native and SDS polyacrylamide gels. The purified PEP contained the core alpha, beta, beta', beta" subunits and five major associated proteins of unknown function, but lacked sigma factors required for promoter recognition. The holoenzyme efficiently recognized a plastid psbA promoter when it was reconstituted from the purified PEP and recombinant plastid sigma factors. Reconstitution of a plastid holoenzyme with individual sigma factors will facilitate identification of sigma factor-specific promoter elements. 相似文献
12.
13.
14.
15.
16.
17.
18.