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Carel J. van Oss 《Preparative biochemistry & biotechnology》2013,43(1):87-89
Abstract Increasing complications in incisional hernia surgery call for novel treatments. A gene expression analysis of injured tissues displays important parameters for tissue regeneration. Until today, no reliable method has been described for a quantitative gene expression analysis of hernia tissues. In this work, a protocol is described for the isolation of DNA‐free total RNA of incisional hernias for the first time. Moreover, real‐time RT PCR assays for collagen type I and III and TGF‐β1 are demonstrated for relative gene expression analyses. Both methods enable relative gene expression analyses of hernia tissues for the first time. 相似文献
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W. B. Elliott 《Preparative biochemistry & biotechnology》2013,43(2):195-196
A previous communication from this laboratory1 as well as one from another3 described the separation of α2-macroglobulin from swine serum. The products from both laboratories contained, in addition to α2-macroglob-ulin, an additional macroglobulin contaminant with α2-globulln mobility. Due to their physicochemical similarity these macroglobulins are not resolved using conventional column procedures such as ion exchange chromatography and gel filtration. Subsequent experiments have shown that immunoelectro-phoretically pure swine α2-macroglobulln is present, in good yield (65%) in the breakthrough effluent of columns of Bio-Gel A-1.5m-Reactive Blue 2 while the contaminating macroglobulin is tightly bound. The production of highly purified swine α2-macroglobulin utilizing this observation is the subject of the present report. The product of the separation was found to be homogeneous when subjected to Immunoelectrophoresis, at a concentration of 14–16 mg/ml, and diffused against antiswlne whole serum antibody. The production of monospecific antibody, a more stringent test for homogeneity, resulted when the purified α2-macroglobulin was injected into rabbits. Physicochemical analyses on the purified product showed that swine and human α2-macro-globulins are true homologs. 相似文献
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