Species of the Paramecium aurelia complex in Russia (western region of European Russia) with molecular characteristics of Paramecium novaurelia

The presence of P. primaurelia, P. biaurelia, P. triaurelia, and P. novaurelia of the P. aurelia complex was revealed in the studied region of Russia. RAPD-PCR fingerprints (band patterns) of newly identified P. novaurelia strains from Russia were compared to those characteristic for the other chosen European strains of the species. The strains revealed intraspecific polymorphism as several groups of genotypes confirming the existence of polymorphism within P. novaurelia.     

The Toxic Symbiont Caedibacter caryophila in the Cytoplasm of Paramecium novaurelia

Endosymbiotic bacteria were observed to inhabit the cytoplasm of the freshwater ciliateParamecium novaurelia. Transmission electron microscopy and toxicity tests with sensitive paramecia showed that the endosymbionts belong to the genusCaedibacter. The bacteria conferred a killer trait to their host paramecia. The production of a proteinaceous inclusion body (“R-body”) in the bacterial cell makes them toxic to other paramecia after they become enclosed in food vacuoles. R-bodies ofCaedibacter sp were associated with phages, which are known in most otherCaedibacter species to code for the R-body proteins. The killer-effect ofP. novaurelia on sensitiveP. caudatum strains was of the “paralysis” type, which is a characteristic of the symbiont speciesCaedibacter caryophila. Until nowC. caryophila was known to inhabit the macronucleus ofParamecium caudatum only. Sequencing of the 16S rRNA-gene proved thatCaedibacter sp from the cytoplasm ofP. novaurelia belongs to the speciesC. caryophila as well. The rDNA-sequence of 1695 bp length differed in a total of only 1 bp from the corresponding gene inC. caryophila from the macronucleus ofP. caudatum. The results indicate that the infection of specific host cell compartments may depend on host genes, but not on different traits of the infecting symbiont species. The occurrence of killer and sensitive paramecia strains together in one pond is discussed with respect to the competitive advantage of the killer trait.     

First American stand of Paramecium novaurelia and intra-specific differentiation of the species

A stand of Paramecium novaurelia was found in Boston Massachusetts, USA, the first on this continent. Molecular studies (RAPD and sequencing ofrRNA [3'SSUrRNA-ITS1, 5' LSU rRNA] and COI mtDNA fragments) of P. novaurelia strains show intra-specific polymorphism within the species as strain clusters characterized by variable relationships.     

Paramecium aurelia species complex in the catchment area of the River Raba mountain course (the Carpathians)

In the water bodies of the Beskid Wyspowy Mts three species of the Paramecium aurelia complex have been found, i.e. P. biaurelia, P. tetraurelia and P. novaurelia. P. tetraurelia, which in Europe is a very rare species, was for the first time recorded in this region of the Carpathians.     

The Paramecium aurelia species complex in Ukraine

New habitats of Paramecium biaurelia were found in the Gorgany Mts, Eastern Carpathians.     

Intraspecific differentiation of Paramecium novaurelia strains (Ciliophora,Protozoa) inferred from phylogenetic analysis of ribosomal and mitochondrial DNA variation

Paramecium novaurelia Beale and Schneller, 1954, was first found in Scotland and is known to occur mainly in Europe, where it is the most common species of the P. aurelia complex. In recent years, two non-European localities have been described: Turkey and the United States of America. This article presents the analysis of intraspecific variability among 25 strains of P. novaurelia with the application of ribosomal and mitochondrial loci (ITS1-5.8S-ITS2, 5′ large subunit rDNA (5′LSU rDNA) and cytochrome c oxidase subunit 1 (COI) mtDNA). The mean distance observed for all of the studied P. novaurelia sequence pairs was p = 0.008/0.016/0.092 (ITS1-5.8S-ITS2/5′LSU rDNA/COI). Phylogenetic trees (NJ/MP/BI) based on a comparison of all of the analysed sequences show that the studied strains of P. novaurelia form a distinct clade, separate from the P. caudatum outgroup, and are divided into two clusters (A and B) and two branches (C and D). The occurrence of substantial genetic differentiation within P. novaurelia, confirmed by the analysed DNA fragments, indicates a rapid evolution of particular species within the Paramecium genus.     

Three new species of Chassalia (Rubiaceae) from the Eastern Arc Mts, Tanzania

Chassalia christineae, sp. nov., and C.bonifacei, sp. nov., from the Nguru Mts, and C.lukwangulensis, sp. nov., from the Uluguru Mts, are described and illustrated. All three species are undershrubs of montane forest.     

Taxonomy of theHieracium alpinum group in the sudeten Mts., the West and the Ukrainian East Carpathians

A taxonomic revision of theHieracium alpinum group (sensu Flora Europaea) in the Sudeten Mts., the West and the Ukrainian East Carpathians is provided. Six species are distinguished in the area studied, viz.Hieracium alpinum, H. halleri, H. augusti-bayeri, H. melanocephalum, H. tubulosum, andH. schustleri. H. alpinum occurs throughout the mountain ranges studied, three other taxa are endemic to the Sudeten Mts. (H. melanocephalum, H. tubulosum, H. schustleri), one is confined to the West Carpathians (H. halleri, in addition to the Alps) and one is endemic to the Ukrainian East Carpathians (H. augusti-bayeri). A diploid cytotype (2n=18) was ascertained inH. alpinum from the Ukrainian East Carpathians and the same chromosome number was found forH. augusti-bayeri. An agamospermous mode of reproduction was confirmed for the triploid cytotype ofH. alpinum, the triploidH. melanocephalum andH. halleri and the tetraploidsH. tubulosum andH. schustleri; the diploid taxa were found to be sexual. Pollen production in diploid taxa is high and pollen grains are homogeneous in size; triploid species, as well as tetraploidH. tubulosum, do not produce polllen; tetraploidH. schustleri has pollen grains of variable size. Data on the ecology and distribution of the species are also given.     

A contribution to the karyology ofCrocus vernus agg. (Iridaceae) from the Iserian Mts. (Sudety Mts., Poland)

The Iserian Mts. form ofCrocus vernus agg. has 2n = 16 and a karyotype clearly deviating fromC. heuffelianus s. lat.     

Multivariate analysis of theSenecio nemorensis group (Compositae) in the Carpathians with a new species from the East Carpathians

Six species and subspecies of theS. nemorensis group from the Carpathians are recognized here, based on morphometric analyses (cluster and discriminant analyses). A new speciesS. ucranicus Hodálová and a new hybridS. ×dominii Hodálová=S. germanicus Wallr.×S. ucranicus Hodálová, occurring in the East Carpathians and the eastern part of the South Carpathians, are described. The vicarious nature ofS. hercynicus Herborg subsp.hercynicus, S. dacicus Hodálová etMarhold andS. ucranicus Hodálová in the Carpathians is documented. A key to theS. nemorensis group in the Carpathians as well as toS. nemorensis L. s. str. is presented.     

Macrophyte biomass in the Kiev reservoir (Ukraine)

This paper presents the results from research of a few macrophyte communities and their dominant stands in the Kiev Reservoir (Ukraine). Largely concentrated on the methodological aspects of biomass sampling in macrophyte communities.     

Salix silesiaca communities in the Fatra Mts. (Central Slovakia)

Two new associations belonging to theSalicion silesiacae within theBetulo carpaticae-Alnetea viridis are described. These are disclimax shrub communities dominated bySalix silesiaca at higher altitudes of the Fatra Mts. (Western Carpathians). Synmorphological, synecological, syntaxonomical, syngenetical and synchorological characteristics of the communities are given. Floristic differences between the montane shrub communities in the Western Carpathians and those in the Sudeten Mts. are summarized in a synthetic table.     

Landscape history,calcareous fen development and historical events in the Slovak Eastern Carpathians

We explored interactions among human activities, landscape development and changes in biotic proxies in two small calcareous spring fens in the Slovak Eastern Carpathians. These date back to cal. a.d. 930. Results of pollen, plant macrofossil, and mollusc analyses were compared with the settlement history. The regional pollen record reflected historical events and changes in the settlement density very well at both study sites. The natural mixed fir-beech-spruce forests with fern undergrowth were suppressed and replaced by light-demanding trees in the periods of high human impact (e.g. Wallachian colonization). The study area was affected several times by wars and raids followed by a consequent decline in the settlement density. Some of these events are well reflected in the pollen records that document tree recovery and decline of cereals, weeds, and pasture indicators. In comparison, only some landscape changes were reflected in the local fen development. Both spring fens originated after deforestation, Ro?kovce around a.d. 1347 and Mirol'a around a.d. 929. The most pronounced change involving the water regime stabilization and undisturbed development of plant and mollusc communities took place after the decline in human impact. We conclude that humans were the main drivers of landscape transformation in the last millennium; they directly created spring-fen ecosystems through deforestation and influenced fen species composition through husbandry activities.     

Middle Jurassic stromatactis mud-mound in the Pieniny Klippen Belt (Western Carpathians)

Summary A stromatactis mud-mound has been found near Slavnické Podhorie in the Czorsztyn Unit of the Pieniny Klippen Belt (Western Carpathians, Slovakia). Its stratigraphic range is Bathonian to Callovian and it is one of the youngest known true stromatactis mud-mounds. The complete shape the mound is not visible since the klippe is a tectonic block encompassed by younger Cretaceous marls. The matrix is micritic to pelmicritic mudstone, wackestone to packstone with pelecypods, brachiopods, ammonites, and crinoids. An important component of the mound is stromatactis cavities that occur as low as the underlying Bajocian-Bathonian crinoidal limestones. The stromatactis cavities are filled by radiaxial fibrous calcite (RFC) as well as in some places by internal sediment and, finally, by clear blocky calcite. Some cavities remain open with empty voids in the centres. In some stromatactis cavities, tests of cavedwelling ostracodsPokornyopsis sp. were found, surrounded by the latest stages of the RFC. This indicates that stromatactis cavities formed an open network enabling migration of the ostracods and their larvae over a period of time. Except in the case of the stromatactis cavities, there are numerous examples of seeming recrystallizationsensu Black (1952) and Ross et al. (1975) and Bathurst (1977). The radiaxial fibrous calcite encloses patches of matrix and isolated allochems. The RFC crystals are oriented perpendicularly to the substrate whether it is a cavity wall or enclosed allochems. This means that the RFC crystals could not grow from the centre of the cavity outward as postulated by Ross et al. (1975). There are also numerous “floating” isolated allochems, which are much smaller than the surrounding RFC crystals. The explanation involving three-dimensional interconnection of allochems seems to be unlikely. In the discussed mud-mound there is a conflict between apparently empty cavities that had to exist in the sediment and seeming “recrystallization” related to the same RFC that forms the initial void filling. The authors favor an alternative explanation of the “recrystallization”. We presume that the allochems served as nucleation points on which the crystals started to grow. Obviously, the allochems and the micritic patches were different from the surrounding material. RFC crystals (either short-or long-bladed) of the “recrystallization” spar grew at the expense of decaying microbial mucillages. The mucus can enclose peloids, allochems, or whole micritic patches that “floated” in the cavity and served as nucleation sites for the RFC crystals. The entire mud-mound represents a microbially bound autochthonous micritic mass; the stromatactis and stromatactis-like cavities originated where purer mucillage patches occurred, giving rise to open spaces. Such features as the morphological variety of stromatactis fabrics, the pervasive penetration of the sparry calcite into matrix, and the enclosure of the “floated” allochems and mudstone patches by sparry calcite, seem to provide support for the presence of mucus aggregates within the mound body. The mucus might be related to protozoans rather than to sponges or other well organized metazoan organisms. Occurrence of the stromatactis cavities in the underlying Bajocian-Bathonian crinoidal limestones support the inference on biological origin of the stromatactis fabrics. The alternative inorganic models of stromatactis origin (e.g., internal erosion or water-escape) are hardly applicable to the sediment formed by crinoidal skeletal detritus.     

Chromosomal differentiation withinCrocus vernus agg. (Iridaceae) in the Carpathian Mts.

Western and eastern Carpathian populations ofCrocus heuffelianus s. lat. (incl.C. scepusiensis) have 2n = 18 but differ in karyotype. While western populations are chromosomally monomorphic, eastern populations exhibit geographical karyotypic differentiation.     

Yersinia species in the dunnock (Prunella modularis) in sub-alpine habitats of the Western Carpathians

The study presents the prevalence of Yersinia species in dunnok Prunella modularis from the sub-alpine zone of the Western Carpathians. Bacteria were detected from cloacal and pharyngeal swabs from 97 specimens using PCR assay. Yersinia enterocolitica showed the highest prevalence (47.4%) from among the determined Yersinia species. Yersinia species (except Y frederiksenii) were detected more frequently in pharyngeal than cloacal samples. The highest prevalence of yersiniosis was detected in April (Yersinia spp. - 80%, Y. enterocolitica - 70%). No statistically differences were observed in the prevalence of Yersinia spp. between males and females and between juveniles and adult birds. Bacterial contamination did not affect body weight or tarsus length.     

Ground beetles of the Ukraine (Coleoptera, Carabidae)

A review of the ground beetles of the Ukrainian fauna is given. Almost 750 species from 117 genera of Carabidae are known to occur in the Ukraine. Approximately 450 species of ground beetles are registered in the Carpathian region. No less than 300 species of ground beetles are found in the forest zone. Approximately 400 species of Carabidae present in the forest-steppe zone are relatively similar in species composition to those in the forest territories. Some 450 species of Carabidae are inhabitants of the steppe zone. Representatives of many other regions of heterogeneous biotopes such as forest, semi desert, intrazonal, etc. can be found in the steppe areas. The fauna of Carabidae (ca. 100 species) of the lowlands of southern Ukraine (sandy biotopes), situated mostly in the Kherson region, is very peculiar. The fauna of the Crimean mountains contains about 300 species. Conservation measures for the Carabidae are discussed.