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1.
Zusammenfassung In vergleichend autoradiographischen Untersuchungen wurde der Einbau von percutan applizierten 3H-Uridin, 3H-Histidin und 3H-Glucose in die wichtigsten Organsysteme (Epidermis, ZNS, Muskeln, Chorda, Leber, Kiemendarm, Darm) von Branchiostoma lanceolatum (Acranier) und Brachydanio rerio (Teleosteer) nach Inkorporationszeiten von 11 min bis zu 7 Tagen verfolgt. Der Stoffwechsel der markierten Substanzen in den einzelnen morphologisch miteinander zu homologisierenden Organen war bei den beiden Spezies sehr ähnlich, bei Branchiostoma allerdings (mit Ausnahme des ZNS) 4–5mal stärker als bei Brachydanio. Bei dem letzteren wurde außerdem eine zeitliche Verzögerung in der Tracer-Aufnahme (lag-Phase) beobachtet. Insbesondere der ZNS-Stoffwechsel von Acraniern zeigte ähnliche Charakteristika wie der von Vertebraten: Verbleib des Hauptanteils der neusynthetisierten RNS im Perikaryon, axonalen Protein-Transport, Vorwiegen der Glykogensynthese in den Nervenfaserendformationen. Allerdings fanden sich im ZNS von Branchiostoma niedrigere Stoffwechselraten als im ZNS von Brachydanio.
Comparative histophysiological investigations of different organs in Branchiostoma lanceolatum (Cephalochordata) and Brachydanio rerio (Teleostei)
Summary Incorporation of 3H-uridine, 3H-histidine and 3H-glucose into some organs (epidermis, CNS, muscles, spinal cord, notochord, liver, gills, intestine) of Branchiostoma lanceolatum (Acrania) and Brachydanio rerio (Teleostei) was investigated by means of comparative autoradiograms following incorporation periods of 11 min to 7 days. The metabolism of the labeled substances in the various homologous organs examined was quite similar, although it was 4 to 5 times higher in Branchiostoma than in Brachydanio; in the latter there was also a delay of tracer incorporation of about 3 hrs, a so-called lag-phase. In particular the metabolism of the CNS of Branchiostoma showed the same characteristics as the CNS of vertebrates, e. g. storage of neuronally synthesized RNA in the neuronal perikarya, axonal flow of proteins, glycogen synthesis in nerve endings. However, metabolic activity of the CNS was lower in Branchiostoma.
Mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
2.
Hans W. Laale 《In vitro cellular & developmental biology. Plant》1981,17(8):701-705
Summary Blastoderm explants fromBrachydanio rerio (Teleostei: Cyprinidae) high blastulas exhibited limited differentiation of optic structures in culture. A number of explants
showed migration of pigmented retinal epithelial cells and formation of monolayers. The findings permit comparative studies
in vitro on phenomena pertaining to pigmented retinal epithelial cell morphology, function, and differentiation.
This investigation was supported by a grant from the Natural Sciences and Engineering Research Council of Canada. 相似文献
3.
Marie-Claude Senut Seema Azher Frank L. Margolis Kamakshi Patel Ahmad Mousa Arshad Majid 《Cell and tissue research》2009,337(1):45-61
Carnosine-like peptides (carnosine-LP) are a family of histidine derivatives that are present in the nervous system of various
species and that exhibit antioxidant, anti-matrix-metalloproteinase, anti-excitotoxic, and free-radical scavenging properties.
They are also neuroprotective in animal models of cerebral ischemia. Although the function of carnosine-LP is largely unknown,
the hypothesis has been advanced that they play a role in the developing nervous system. Since the zebrafish is an excellent
vertebrate model for studying development and disease, we have examined the distribution pattern of carnosine-LP in the adult
and developing zebrafish. In the adult, immunoreactivity for carnosine-LP is specifically concentrated in sensory neurons
and non-sensory cells of the olfactory epithelium, the olfactory nerve, and the olfactory bulb. Robust staining has also been
observed in the retinal outer nuclear layer and the corneal epithelium. Developmental studies have revealed immunostaining
for carnosine-LP as early as 18 h, 24 h, and 7 days post-fertilization in, respectively, the olfactory, corneal, and retinal
primordia. These data suggest that carnosine-LP are involved in olfactory and visual function. We have also investigated the
effects of chronic (7 days) exposure to carnosine on embryonic development and show that 0.01 μM to 10 mM concentrations of
carnosine do not elicit significant deleterious effects. Conversely, treatment with 100 mM carnosine results in developmental
delay and compromised larval survival. These results indicate that, at lower concentrations, exogenously administered carnosine
can be used to explore the role of carnosine in development and developmental disorders of the nervous system.
This research was supported in part by an Intramural Research Grant Program Award from Michigan State University (no. 06-IRGP-899
to M.C.S.) and a grant from the National Institutes of Health (no. DC-03112 to F.L.M.). 相似文献
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6.
Summary A qualitative and quantitative analysis of lectin-binding sites has been undertaken on spermatozoa recovered from different regions of the epididymis of the goat (Capra indicus) using fluorescein isothiocyanate-linked lectins (Bauhinia purpurea BPA, Concanavalin A Con A, Dolichos biflorus DBA, Maclura pomifera MPA, Arachis hypogaea or peanut agglutinin PNA, Glycine max or soyabean agglutinin SBA, Ulex europaeus UEA, and Triticum vulgaris or wheat-germ agglutinin WGA), in conjunction with scanning and transmission electron microscopy, and freeze-fracture techniques. Flow cytometric analysis has also been used to quantitize binding affinity. Spermatozoa from caput to cauda epididymidis show no significant variation in lectinbinding ability, but the samples removed from the corpus epididymidis contain a greater number of binding sites. The passage of spermatozoa through the epididymidis is accompanied by a redistribution of the plasma membrane lectin-receptors covering the sperm head and tail. Receptors for BPA, DBA, PNA and SBA are specifically restricted to the anterior region of the acrosome in caudal spermatozoa. Freeze-fracture replicas, examined to study changes in organisation of intramembranous particles of the plasma membrane during sperm maturation, reveal distinct changes in their distribution in the acrosome, post-acrosome and spermatozoon tail, especially in the corpus and cauda epididymidis. 相似文献
7.
Caudal neurosecretory system of the zebrafish: ultrastructural organization and immunocytochemical detection of urotensins 总被引:1,自引:0,他引:1
The caudal neurosecretory system is described here for the first time in the zebrafish, one of the most important models used to study biological processes. Light- and electron-microscopical approaches have been employed to describe the structural organization of Dahlgren cells and the urophysis, together with the immunohistochemical localization of urotensin I and II (UI and UII) peptides. Two latero-ventral bands of neuronal perikarya in the caudal spinal cord project axons to the urophysis. The largest secretory neurons (~20 μm) are located rostrally. UII-immunoreactive perikarya are much more numerous than those immunoreactive for UI. A few neurons are immunopositive for both peptides. Axons contain 75-nm to 180-nm dense-core vesicles comprising two populations distributed in two axonal types (A and B). Large dense vesicles predominate in type A axons and smaller ones in type B. Immunogold double-labelling has revealed that some fibres contain both UI and UII, sometimes even within the same neurosecretory granule. UII is apparently the major peptide present and predominates in type A axons, with UI predominating in type B. A surprising finding, not previously reported in other fish, is the presence of dense-core vesicles, similar to those in neurons, in astrocytes including their end-feet around capillaries. Secretory type vesicles are also evident in ependymocytes and cerebrospinal-fluid-contacting neurons in the terminal spinal cord. Thus, in addition to the urophysis, this region may possess further secretory systems whose products and associated targets remain to be established. These results provide the basis for further experimental, genetic and developmental studies of the urophysial system in the zebrafish. 相似文献
8.
Summary Observation of the cortical region of oocytes of Bufo arenarum by transmission electron microscopy reveals modifications on their surface and in the contents of the cortical granules (CG) during activation. In non-activated oocytes only amorphous cortical granules (ACG) can be observed. Activated oocytes display ACG, intermediate cortical granules containing both amorphous and membranous material (ICG), and a third type containing only membranous material (MCG). During exocytosis, CG release their contents into the perivitelline space, where the amorphous and membranous materials are found. The three types of CG found during oocyte activation suggest transformation of ACG to MCG and indicate that the different components of the cortical granules, when released into the perivitelline space, might play different roles in prevention of polyspermy.Members of the Scientific Research Career of CONICET, R. Argentina. 相似文献
9.
Based on light and transmission electron-microscopic observations on erupting first-generation teeth in the zebrafish, Danio rerio, we propose a biphasic mechanism for tooth eruption: (1). formation of an epithelial crypt prior to eruption of the tooth, possibly as a result of constraints in the epithelium resulting from the growth of adjacent tooth germs, and (2). detachment of cellular interdigitations both within the pharyngeal epithelium, at the pharyngeal epithelium/enamel organ boundary, and between the outer and inner dental epithelium, resulting in the exposure of the tooth tip in the crypt, immediately after tooth ankylosis. Later, further detachment of interdigitations between the inner and the outer enamel epithelium unfolds the epithelium even more and leads to a more pronounced exposure of the tooth tip. The presence of small patches of non-collagenous matrix on the outer surface of the tooth close to where it merges with the attachment bone is interpreted as a device to prevent complete detachment of the enamel organ. The biphasic nature of the mechanism for tooth eruption is supported by observations on in vitro cultured heads. First-generation teeth develop normally and crypts are formed, as under in vivo conditions, but the teeth fail to erupt. Taken together, our observations suggest that epithelial remodelling plays a crucial role in eruption of the teeth in this model organism. 相似文献
10.
R. S. Pearce 《Planta》1985,166(1):1-14
Seedlings of Triticum aestivum L. cv. Neepawa were slowly drought-stressed by witholding water after sowing in pots. Leaf extension stopped during development of the third leaf. Damage was assessed by rewatering the pots and measuring regrowth; 1–5 d after growth stopped, rewatering induced significant regrowth within several hours; 6–13 d after growth stopped, regrowth was delayed; from 14 d after growth stopped, no regrowth occurred after rewatering. Leaf bases were excised from the drought-stressed seedlings during this period of increasing damage, and were freeze-etched.Intramembranous particles (IMP) were evenly scattered in the plasma membrane in those plants which regrew immediately after rewatering. In the plants which regrew after a delay or which did not regrow on rewatering, there were patches without IMP in plasma membrane, nuclear envelope, and other membranes. Plasma membrane, nuclear envelope and possibly other membranes were sometimes partly replaced by vesicles, possibly formed from the original membrane. Such vesiculation occurred in a few cells in plants which survived the stress with a delayed regrowth, and was commoner in the plants which did not recover. The results support the idea that slow drought induces IMP-free patches in membranes including the plasma membrane, this induces membrane reorganisation including vesiculation of membranes and coagulation of protoplasm, and that these are expressed as delayed or failed regrowth. Some IMP-free patches in the plasma membrane had a faint ordered sub-structure, possibly a hexagonal lipid phase. Such patches were infrequent and IMP sometimes occurred in areas of plasma membrane having an apparently similar sub-structure. Thus the IMP-free patches could not be explained by a lamellar-hexagonal phase transition. As the stress became damaging, vesicles and endoplasmic reticulum accumulated immediately next to the plasma membrane. Mainly during the early period of damaging stress (6–10 d after growth stopped), depressions, invaginations, and rarer lesions occurred in the plasma membrane, sometimes associated with some of the IMP-free patches. In the same period, many nuclear envelopes had exceptionally large nuclear pores.Abbreviations E
exoplasmic
- IMP
intramembranous particles
- P
protoplasmic 相似文献
11.
Cadherin cell adhesion molecules play crucial roles in vertebrate development. Most studies have focused on examining the functions of classical type I cadherins (e.g., cadherin-2) in the development of vertebrates. Little information is available concerning the function of classical type II cadherins (e.g., cadherin-7) in vertebrate development. We have previously shown that cadherin-7 mRNA exhibits a dynamic expression pattern in the central nervous system and notochord in embryonic zebrafish. To gain insight into the role of cadherin-7 in the formation of these structures, we analyzed their formation in zebrafish embryos injected with cadherin-7-specific antisense morpholino oligonucleotides (MO). Notochord development was severely disrupted in MO-injected embryos, whereas gross defects in the development of the central nervous system were not detected in MO-injected embryos. Our results thus demonstrate that cadherin-7 plays an important role in the normal development of the zebrafish notochord. 相似文献
12.
Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants 总被引:1,自引:0,他引:1
Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was conducted on stage III and stage V zebrafish oocytes. Volumetric changes of stage III oocytes in different concentrations of sucrose were measured after 20 min exposure at 22 degrees C and the osmotically inactive volume of the oocytes (Vb) was determined using the Boyle-van't Hoff relationship. Volumetric changes of oocytes during exposure to different cryoprotectant solutions were also measured. Oocytes were exposed to 2 M dimethyl sulphoxide (DMSO), propylene glycol (PG), and methanol for 40 min at 22 degrees C. Stage III oocytes were also exposed to 2 M DMSO at 0 degrees C. Oocyte images were captured on an Olympus BX51 cryomicroscope using Linkham software for image recording. Scion Image was used for image analysis and diameter measurement. The experimental data were fitted to a two-parameter model using Berkeley Madonna 8.0.1 software. Hydraulic conductivity (L(p)) and solute (cryoprotectant) permeability (Ps) were estimated using the model. The osmotically inactive volume of stage III zebrafish oocytes was found to be 69.5%. The mean values+/-SE of Lp were found to be 0.169+/-0.02 and 0.196+/-0.01 microm/min/atm in the presence of DMSO and PG, respectively, at 22 degrees C, assuming an internal isosmotic value for the oocyte of 272 mOsm. The Ps values were 0.000948+/-0.00015 and 0.000933+/-0.00005 cm/min for DMSO and PG, respectively. It was also shown that the membrane permeability of stage III oocytes decreased significantly with temperature. No significant changes in cell volume during methanol treatment were observed. Fish oocyte membrane permeability parameters are reported here for the first time. The Lp and Ps values obtained for stage III zebrafish oocytes are generally lower than those obtained from successfully cryopreserved mammalian oocytes and higher than those obtained with fish embryos and sea urchin eggs. It was not possible to estimate membrane permeability parameters for stage V oocytes using the methods employed in this study because stage V oocytes experienced the separation of outer oolemma membrane from inner vitelline during exposure to cryoprotectants. 相似文献
13.
Summary The olfactory epithelium of the three-spined stickleback (Gasterosteus aculeatus) and the nine-spined stickleback (Pungitius pungitius) has been studied with a conventional histochemical and a novel immunological staining technique. In both species, the sensory epithelium is arranged in folds separated by non-sensory epithelial tissue. In the nine-spined stickleback, intrinsic folds consisting of non-sensory cells are found in the apical part of the sensory epithelium where they divide the surface of the sensory epithelium into small islets. These non-sensory cells are non-ciliated, flattened and piled on top of each other; they contain numerous electron-translucent vesicles. The intrinsic folds are absent from the sensory epithelium of the three-spined stickleback. In both species, axons of receptor cells form a layer of fibers in the sensory epithelium immediately above the basal cells. In the three-spined stickleback, thick branches of the olfactory nerve are frequently found in this layer. These branches are only occasionally observed in the sensory epithelium of the nine-spined stickleback. Thus, the three-spined stickleback and the nine-spined stickleback show considerable differences in the organization of the sensory regions of the olfactory epithelium. 相似文献
14.
Interstitial cells from the testis of the trout (Oncorhynchus mykiss) in vivo and in primary culture
Maurice Loir 《Cell and tissue research》1990,261(1):133-144
Summary In the testis of the trout, while no changes are apparent in myoid cells at any stage of maturation, Leydig cells display striking structural alterations when observed at different periods of the reproductive cycle. Spermiating testes contain fully differentiated Leydig cells. In regressed testes and those involved in spermatogenesis, poorly differentiated Leydig cells are mixed with cells ranging structurally from normal Leydig cells to fibroblast-like elements. After 3–4 days in culture the myoid cells/fibroblasts progressively acquire the ability to proliferate and then show a positive reaction for 3-hydroxysteroid dehydrogenase. During the same period they undergo structural changes reflecting the emergence of a steroidogenic activity. These changes occur concomitantly with an increase in progestagen secretion. These data suggest that, in vivo, Leydig cells degenerate at the end of a cycle, being then replaced by fibroblastic precursor cells capable of division and differentiation into steroidogenic cells. 相似文献
15.
The development of the frontal bone and the formation of the first head scales are described during post-embryonic ontogeny of Hemichromis bimaculatus, using light and transmission electron microscopy. The frontal bone originates close to the cartilaginous taenia marginalis in a loose mesenchymal cell condensation (=primordium) lying 1 m from the epidermis with which it establishes no cell contacts. The anlage appears at 4.2 mm standard length (SL) in the form of the membranodermal component of the bone, and extends first over the brain and then over the eye; the neurodermal component forms later to surround the supraorbital canal. The first head scales appear at 10.0 mm SL in a dense cell condensation (papilla) adjoining the epidermal-dermal junction and, once formed, remain in this position. In both organs, the initial matrix is similarly composed of woven-fibred bone that soon mineralizes in a similar manner to other dermal elements. In some areas of the frontal bone, parallel-fibred bone is deposited unequally on both surfaces, whereas isopedine is deposited in scales on the deep surface only. Osteoblastic features confirm this eccentric growth. Differences in the shape, organization and localization of the mesenchymal condensations giving rise to the frontal bone and to the scale reflect the existence of two types of dermal cell condensations. Our data are compared with those available for the post-cranial dermal skeleton of fishes both from a developmental and structural viewpoint. Structural differences in the matrices of the frontal bone and scales are discussed in a phylogenetic perspective. 相似文献
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17.
Mlh1 is a member of DNA mismatch repair (MMR) machinery and is also essential for the stabilization of crossovers during the
first meiotic division. Recently, we have shown that zebrafish mlh1 mutant males are completely infertile because of a block in metaphase I, whereas females are fertile but have aneuploid progeny.
When studying fertility in males in a two-fold more inbred background, we have however observed low numbers of fertilized
eggs (approximately 0.4%). Histological examination of the testis has revealed that all spermatogenic stages prior to spermatids
(spermatogonia, primary spermatocytes, and secondary spermatocytes) are significantly increased in the mutant, whereas the
total weight of spermatids and spermatozoa is highly decreased (1.8 mg in wild-type vs. 0.1 mg in mutants), a result clearly
different from our previous study in which outbred males lack secondary spermatocytes or postmeiotic cells. Thus, a delay
of both meiotic divisions occurs rather than complete arrest during meiosis I in these males. Eggs fertilized with mutant
sperm develop as malformed embryos and are aneuploid making this male phenotype much more similar to that previously described
in the mutant females. Therefore, crossovers are still essential for proper meiosis, but meiotic cell divisions can progress
without it, suggesting that this mutant is a suitable model for studying the cellular mechanisms of completing meiosis without
crossover stabilization.
Marcelo C. Leal and Harma Feitsma contributed equally to this work.
This work was supported by the Brazilian Foundation CAPES, the Cancer Genomics Center (Nationaal Regie Orgaan Genomics), the
European Union-funded FP6 Integrated Project ZF-MODELS, and Utrecht University. 相似文献
18.
A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission electron microscopy was used to compare cellular and extracellular features of tooth germs developing in vitro with the situation in vivo. Head explants of H. bimaculatus from 120 h post-fertilization (hPF) to 8.5 days post-fertilization (dPF) and of zebrafish from 45 hPF to 79 hPF and adults kept in culture for 3, 4 or 7 days revealed that tooth germs developed in vitro from explants in which the buccal or pharyngeal epithelium was apparently undifferentiated and, when present at the time of explantation, they continued their development up to a stage of attachment. In addition, the medium allowed the morphogenesis and cytodifferentiation of the tooth germs similar to that observed in vivo and the establishment of a dental pattern (place and order of tooth appearance and of attachment) that mimicked that in vivo. Organotypic culture in serum-free conditions thus provides us with the means of studying epithelial-mesenchymal interactions during tooth development in teleost fish and of analysing the genetic control of either mandibular or pharyngeal tooth development and replacement in these polyphyodont species. Importantly, it allows heads from embryonically lethal (zebrafish) mutants or from early lethal knockdown experiments to develop beyond the point at which the embryos normally die. Such organotypic culture in serum-free conditions could therefore become a powerful tool in developmental studies and open new perspectives for craniofacial research.The in vitro infrastructure at the Ghent laboratory was financed through a grant of the Bijzonder Onderzoeksfonds of Ghent University (BOF: 01102995) and a Krediet aan navorsers (no. 31513695) of the Fonds voor Wetenschappelijk onderzoek (FWO-Vlaanderen). This study also benefitted from an exchange program between the Centre National de Recherche Scientifique (CNRS) and the Ministerie van de Vlaamse Gemeenschap. Research performed by C. Van der heyden was partly financed through a specialization grant of the Flemish Institute for the Advancement of Scientific-Technological Research in Industry (IWT). 相似文献
19.
Serotonin- and ten peptide-immunoreactive (IR) cell types were identified in the digestive tract of sea bass (Dicentrarchus labrax L.) larvae of four morphofunctional phases ranging in age from hatching to 61 days. The sequence of appearance and location of endocrine cells during ontogenetic development of the larvae was determined. The differentiation of endocrine cells followed a distal-proximal gradient in the gut which paralleled the morphofunctional differentiation. Serotonin-IR cells were identified in the last portion of the digestive tract from phase I onwards and in the gastric region from phase III, before these regions were morphofunctionally differentiated; met-enkephalin-IR cells were identified from phase II onwards in both the differentiated rectum and the undifferentiated intestine; cholecystokinin (CCK)- and synthetic human gastrin-34-IR cells were located only in the intestine and first found in the undifferentiated intestine of phase II; human gastrin-17-, peptide YY (PYY)- and neuropeptide Y (NPY)-IR cells appeared in the intestine from phase II and in stomach in phase IV, when it showed gastric glands; pancreatic polypeptide (PP)- and glucagon-IR cells were observed in both intestine and stomach, but insulin- and somatostatin-IR cells only in stomach, from phase III, during which the intestine but not the stomach was differentiated. PP- and PYY-, PP- and glucagon-, and PYY- and glucagon-like immunoreactivities coexisted from their first appearance in some cells of the gut. 相似文献
20.
The primordial cord and the primitive, single and primordial islets present in the 3 earliest stages of the developing endocrine pancreas of sea bass were studied ultrastructurally. The primordial cord consisted of type I and II cells and was included in the gut. Besides these cell types, X cells were seen in the primitive islet. The single islet was made up of type I, II, III and IV cells. A correlation between these endocrine cell-types and cells previously identified immunocytochemically, was established. Type I, II, III and IV cells, correlated respectively with SST-25-, insulin-, SST-14- and glucagon-immunoreactive cells, and could be related to the D1, B, D2 and A cells, respectively, of older larvae and adult sea bass. Each cell type shows characteristic secretory granules from its first appearance. A progressive development of the organelles and an increase in the number and size of the secretory granules, whose ultrastructure also varied, was observed in the endocrine cells of the primordial cord and the succeeding islets. In 25-day-old larvae at the beginning of the fourth developmental stage, the primordial islet, the first ventral islet found, was close to a pancreatic duct and blood vessel, and consisted of type I and II cells whose ultrastructure was similar to that of the type I and II cells in the primordial cord. These data suggest a ductular origin for the pancreatic endocrine cells in the ventral pancreas. It is suggested that although endocrine cells undergo mitosis, their increase in number during the earliest development stages is principally due to the differentiation of surrounding cells. 相似文献