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1.
Squishy lipids: Temperature effects on the betaine and galactolipid profiles of a C18/C18 peridinin‐containing dinoflagellate,Symbiodinium microadriaticum (Dinophyceae), isolated from the mangrove jellyfish,Cassiopea xamachana
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Jeffrey D. Leblond Manoj Khadka Linda Duong Jeremy L. Dahmen 《Phycological Research》2015,63(3):219-230
Previous work from our laboratory has shown dinoflagellates, which possess the carotenoid peridinin, have been divided into two clusters based on plastid galactolipid fatty acid composition. In one cluster major forms of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), lipids that comprise the majority of photosynthetic membranes, were C18/C18 (sn‐1/sn‐2), with octadecapentaenoic [18:5(n‐3)] and octadecatetraenoic [18:4(n‐3)] acid as principal fatty acids. The other cluster contained C20/C18 major forms, with eicosapentaenoic acid [20:5(n‐3)] being the predominant sn‐1 fatty acid. In this study, we have found that Symbiodinium microadriaticum isolated from the jellyfish, Cassiopea xamachana, when grown at 30°C, produced MGDG and DGDG with a more saturated fatty acid, 18:4(n‐3), at the sn‐2 carbon than when grown at 20°C where 18:5(n‐3) predominates. This modulation of the sn‐2 fatty acid's level of saturation is mechanistically similar to what has been observed in Pyrocystis, a C20/C18 dinoflagellate. We have also examined the effect of growth temperature on the betaine lipid, diacylglycerylcarboxyhydroxymethylcholine (DGCC), which has been observed by others to be the predominant non plastidial polar lipid in dinoflagellates. Temperature effects on it were minimal, with very few modulations in fatty acid unsaturation as observed in MGDG and DGDG. Rather, the primary difference seen at the two growth temperatures was the alteration of the amount of minor forms of DGCC, as well as a second betaine lipid, diacylglyceryl‐N,N,N‐trimethylhomoserine. 相似文献
2.
ABSTRACTThere is a dearth of surveys examining the direct effects of temperature on red algal galactolipids, and none which examine regiochemistry modulation with respect to growth temperature. Therefore, forms of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), the two most commonly found galactolipids in chloroplast membranes, were determined in two model red algae, Polysiphonia sp. and Porphyridium sp., via positive-ion electrospray ionization/mass spectrometry (ESI/MS) and ESI/MS/MS. We sought to compare modulation of galactolipid forms in response to growth temperature between these two red algae and selected descendants with red algal plastid ancestry, and have proposed the following hypothesis: Polysiphonia sp. and Porphyridium sp. would modulate desaturations in the sn-2 position in accordance with previously examined descendant organisms. It was observed that both red algae produced C20/C16 (sn-1/sn-2 regiochemistry) and C20/C20 forms of MGDG and DGDG as their most abundant galactolipids under two growth temperatures, 20°C and 30°C. Furthermore, temperature-induced modulation of the major forms of MGDG and DGDG was more complex than what has been observed previously in selected representatives of red algal plastid ancestry. Porphyridium sp. modulated levels of desaturation in the sn-1 position of C20/C16 forms of MGDG and DGDG and in the sn-1 and sn-2 positions of C20/C20 forms of MGDG and DGDG. Polysiphonia sp. displayed trends suggesting it modulates levels of desaturation in the sn-1 and sn-2 positions of C20/C20 forms of MGDG and DGDG, thus indicating a different approach to regulating plastid membrane fluidity from that which has been observed in algae with secondary, red algae-derived plastids. 相似文献
3.
Despite their importance in marine and freshwater microalgal assemblages, cold-adapted dinoflagellates have been the subject of few comprehensive lipid studies, particularly with respect to those lipids that comprise plastid membranes. In an effort to understand the differences between warm- and cold-adapted dinoflagellate glycolipid composition, four peridinin-containing, cold-adapted dinoflagellates were surveyed for intact forms of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), two common plastid lipids, using positive-ion electrospray ionization/mass spectrometry (ESI/MS) and electrospray ionization/mass spectrometry/mass spectrometry (ESI/MS/MS). It was determined that the dominant forms of MGDG and DGDG in these cold-adapted, peridinin-containing dinoflagellates possessed C18 fatty acids and did not, with the exception of a 20:5/18:5 form of DGDG in a cold-adapted Gymnodinium sp. from the Baltic Sea, have C20 fatty acids. This finding is in contrast to an earlier study of 35 peridinin-containing, warm-adapted dinoflagellates, which discovered a cluster dominated by C18 fatty acids and a cluster dominated by both C20 and C18 fatty acids. The key difference in MGDG and DGDG production between the former group and the cold-adapted dinoflagellates examined in this study is that the cold-adapted species’ DGDG fatty acids were less saturated. Each cold-adapted dinoflagellate possessed both 18:5/18:5 and 18:5/18:4 DGDG, while most of the warm-adapted dinoflagellates contained only 18:5/18:4 DGDG. This survey also revealed the presence of a putative 18:1/14:0 trigalactosyldiacylglycerol (TGDG) as a dominant glycolipid in Gymnodinium sp. TGDG, previously unreported in dinoflagellates, was also discovered in Gymnodinium sp. in the forms of 18:1/16:0 and 18:1/18:1 TGDG, as minor lipids. Since the fatty acids associated with TGDG are not those found with dominant forms of MGDG or DGDG, TGDG may be produced by a different biosynthetic pathway. 相似文献
4.
Raphidophyte algae (Raphidophyceae) can be divided according to pigment composition and plastid ancestry into two categories, brown‐ and green‐pigmented taxa. We sought to examine if there are any biochemical differences in plastid lipid composition between the two groups. To this end, the composition and positional distribution of fatty acids of the chloroplast lipids, mono‐ and digalactosyldiacylglycerol (MGDG and DGDG, respectively), were examined using positive‐ion electrospray/mass spectrometry (ESI/MS) and electrospray/mass spectrometry/mass spectrometry (ESI/MS/MS). Brown‐pigmented strains from the genera Chattonella, Fibrocapsa, and Heterosigma primarily consisted of 20:5/18:4 (sn‐1/sn‐2) MGDG and 20:5/18:4 DGDG, while isolates of the green‐pigmented raphidophyte Gonyostomum semen (Ehrenb.) Diesing contained these as well as 18:3/18:4 MGDG and DGDG, thus underscoring its green algal plastid lineage. Although previously unseen without the regiochemical information provided by ESI/MS/MS, Chattonella subsalsa Biecheler possessed 20:5/18:3 DGDG as a major form, a potential biosynthetic intermediate in the production of 20:5/18:4 DGDG. These results provide a modern interpretation of the fatty acid regiochemistry of MGDG and DGDG. 相似文献
5.
Chromera velia is a recently discovered, photosynthetic, free-living alveolate that is the closest free-living relative to non-photosynthetic apicomplexan parasites. Most plastids, regardless of their origin, have membranes composed chiefly of two galactolipids, mono- and digalactosyldiacylglycerol (MGDG and DGDG, respectively). Because of the hypothesized shared red algal origin between the plastids of C. velia and dinoflagellates, our primary objectives were to examine how growth temperature affects MGDG and DGDG composition via positive-ion electrospray/mass spectrometry (ESI/MS) and positive ion/electrospray/mass spectrometry/mass spectrometry (ESI/MS/MS), and to examine galactolipid biosynthetic genes to determine if shared ancestry translates into shared MGDG and DGDG composition. When growing at 20°C, C. velia produces eicosapentaenoic acid-rich 20:5(n-3)/20:5(n-3) (sn-1/sn-2) MGDG and 20:5(n-3)/20:5(n-3) DGDG as its primary galactolipids, with relative percentage compositions of approximately 35 and 60%, respectively. At 30°C these are lessened by approximately 5 and 8%, respectively, by the corresponding production of 20:5/20:4 forms of these lipids. The presence of 20:5 at the sn-1 position is similar to what has been observed previously in a cluster of peridinin-containing dinoflagellates, but the presence of 20:5(n-3) at the sn-2 position is extremely rare. Thus, the forms of MGDG and DGDG in C. velia displayed similarities and differences to what has been observed in peridinin-containing dinoflagellates, such as Lingulodinium polyedrum, which produces 20:5/18:5 and 20:5/18:4 as the major forms of MGDG and DGDG. We develop conceptual models from the galactolipids observed and galactolipid-relevant gene annotations to explain the presence of polyunsaturated fatty acid-containing MGDG and DGDG in both L. polyedrum and C. velia. 相似文献
6.
V. Joshua Dodson Jeremy L. Dahmen Jean‐Luc Mouget Jeffrey D. Leblond 《Phycological Research》2013,61(3):199-207
Diatoms are one of the largest groups of primary producers in the oceans, yet despite their environmental importance little is known about their plastidial lipid biochemistry. It has been previously reported that Skeletonema species contain primarily C16/C16 and C20/C16 forms of mono‐ and digalactosyldiacylglycerol (MGDG and DGDG, respectively). Likewise, it was also reported that Phaeodactylum tricornutum contains primarily C16/C16 and C20/C20 forms of MGDG and DGDG. We seek to relate their studies to other diatoms, both in the centrics and pennates, with particular focus on the marennine‐producing pennate diatom, Haslea ostrearia. To this end, the composition and positional distribution of fatty acids of MGDG and DGDG were examined using positive‐ion electrospray ionization/mass spectrometry (ESI/MS). Two centric diatoms, Skeletonema marinoi and Thalassiosira weissflogii, and the pennate diatom, P. tricornutum, contained primarily C20/C16 (sn‐1/sn‐2) and C18/C16 forms of MGDG and DGDG. The other pennate diatoms, H. ostrearia and Navicula perminuta, contained primarily C18/C16 or C18/C18 forms of MGDG and DGDG, indicating a previously unrecognized fatty acid diversity in diatom MGDG and DGDG. 相似文献
7.
Temperature modulation and the presence of C20 fatty acids in mono‐ and digalactosyldiacylglycerol of Euglena gracilis and Lepocinclis acus: A modern interpretation of euglenid galactolipids using positive‐ion electrospray ionization/mass spectrometry
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Mono‐ and digalactosyldiacylglycerol (MGDG and DGDG, respectively) are important galactolipids that comprise photosynthetic membranes in almost all photosynthetic organisms. Intact forms of MGDG and DGDG of Euglena gracilis and Lepocinclis acus, two example euglenids with secondary plastids of green algal origin, were elucidated with fatty acid regiochemistry via positive‐ion electrospray ionization/mass spectrometry at two growth temperatures. At 20°C, E. gracilis and L. acus produced predominantly 18:3/16:4 (sn‐1/sn‐2) MGDG, whereas at 30°C this was supplanted by 18:2/16:2 MGDG. At both temperatures were also observed a variety of other MGDG and DGDG forms, including C20 fatty acid‐containing forms not expected in a green algal‐derived plastid. In addition to providing structural details of MGDG and DGDG not available in past studies, these results suggest a previously unknown relationship between these two organisms and the red algae. This study also illustrates that temperature modulation of galactolipids occurs via modification of unsaturation of both the sn‐1 and sn‐2 fatty acids; this is fundamentally different from previously published studies from our laboratory on other algal classes. 相似文献
8.
Jeffrey D. Leblond Spencer L. McDaniel Shelby D. Lowrie Manoj Khadka Jeremy Dahmen 《欧洲藻类学杂志》2019,54(1):78-90
Previous studies have shown that dinoflagellates with different plastid ancestries have distinct differences in the fatty acid compositions and regiochemistries of their chloroplast-associated galactolipids, mono- and digalactosyldiacylglycerol (MGDG and DGDG, respectively), thus reflecting plastid origin as a major factor in plastid membrane composition. Specifically, dinoflagellates with aberrant plastids (e.g. Karenia brevis, Kryptoperidinium foliaceum and Lepidodinium chlorophorum) possess certain MGDG- and DGDG-associated fatty acids which are not found in peridinin-containing dinoflagellates (the largest group of photosynthetic dinoflagellates with a red algal plastid ancestry which is thought to be an evolutionary precursor to aberrant plastids), but which are common to other algal groups. For example, hexadecatetraenoic acid (16:4(n-3)) is common to green algae and is found in the MGDG and DGDG of L. chlorophorum, which agrees with its green algal plastid ancestry, while hexadecatrienoic acid (16:3) and hexadecadienoic acid (16:2) are found in the MGDG and DGDG of K. foliaceum, which agrees with its diatom plastid ancestry. Notably, 16:4 has been found by others in the total fatty acids and galactolipids of Karenia mikimotoi, but in no other examined members of the Kareniaceae (all of which have plastids of haptophyte origin). However, these findings lack information as to the regiochemistry of 16:4. We have utilized positive-ion electrospray ionization/mass spectrometry (ESI/MS) and ESI/MS/MS to demonstrate that 16:4, which aside from L. chlorophorum is not found conclusively in the MGDG and DGDG of any other dinoflagellates examined to date irrespective of plastid ancestry, is found in K. mikimotoi as 18:5/16:4 (sn-1/sn-2 regiochemistry) MGDG and DGDG, and that its presence is not modulated (i.e. does not become more saturated) with an increase in growth temperature. Considering an aberrant pigment composition as described by others, we present a perspective where galactolipid-associated 16:4 in K. mikimotoi indicates a plastid ancestry more convoluted than for other members of the Kareniaceae. 相似文献
9.
Biosynthesis and desaturation of prokaryotic galactolipids in leaves and isolated chloroplasts from spinach 总被引:2,自引:1,他引:2
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Mono- and digalactosyldiacylglycerol (MGDG and DGDG) were isolated from the leaves of sixteen 16:3 plants. In all of these plant species, the sn-2 position of MGDG was more enriched in C16 fatty acids than sn-2 of DGDG. The molar ratios of prokaryotic MGDG to prokaryotic DGDG ranged from 4 to 10. This suggests that 16:3 plants synthesize more prokaryotic MGDG than prokaryotic DGDG. In the 16:3 plant Spinacia oleracea L. (spinach), the formation of prokaryotic galactolipids was studied both in vivo and in vitro. In intact spinach leaves as well as in chloroplasts isolated from these leaves, radioactivity from [1-14C]acetate accumulated 10 times faster in MGDG than in DGDG. After 2 hours of incorporation, most labeled galactolipids from leaves and all labeled galactolipids from isolated chloroplasts were in the prokaryotic configuration. Both in vivo and in vitro, the desaturation of labeled palmitate and oleate to trienoic fatty acids was higher in MGDG than in DGDG. In leaves, palmitate at the sn-2 position was desaturated in MGDG but not in DGDG. In isolated chloroplasts, palmitate at sn-2 similarly was desaturated only in MGDG, but palmitate and oleate at the sn-1 position were desaturated in MGDG as well as in DGDG. Apparently, palmitate desaturase reacts with sn-1 palmitate in either galactolipid, but does not react with the sn-2 fatty acid of DGDG. These results demonstrate that isolated spinach chloroplasts can synthesize and desaturate prokaryotic MGDG and DGDG. The finally accumulating molecular species, MGDG(18:3/16:3) and DGDG(18:3/16:0), are made by the chloroplasts in proportions similar to those found in leaves. 相似文献
10.
Jeffrey D. Leblond Hermina Ilea Timofte Shannon A. Roche Nicole M. Porter 《Phycological Research》2010,58(3):222-229
Glaucocystophytes are freshwater algae that possess an almost‐intact cyanobacterium, referred to as a cyanelle, as their photosynthetic organelle. Because the cyanelle represents an intermediate state in plastid evolution, glaucocystophytes have been the subject of several studies to characterize the genetics and biochemistry of their cyanelles. However, only a small handful of older studies exist on the composition of their lipids, particularly two major plastid lipids, mono‐ and digalactosyldiacylglycerol (MGDG and DGDG, respectively), found in all photosynthetic life. Our study has used a modern mass spectrometry approach, namely positive‐ion electrospray ionization/mass spectrometry/mass spectrometry, to provide a fresh interpretation of the MGDG and DGDG composition of the species, Cyanophora paradoxa Korshikov and Glaucocystis nostochinearum Itzigsohn, representing two glaucocystophyte genera. We have found that the major forms of MGDG and DGDG (with sn‐1/sn‐2 regiochemistry) are 20:5/16:0 MGDG, 20:5/20:5 MGDG, 20:5/16:0 DGDG, and 20:5/20:5 DGDG. A comparison of these four forms, along with other more minor forms of MGDG and DGDG, to two examples of cyanobacteria has revealed that glaucocystophytes do not share intact forms of MGDG and DGDG with extant cyanobacteria, but may have maintained certain C16 and C18 cyanobacterial fatty acids. 相似文献
11.
Jeffrey D. Leblond Braedyn E. Hollingsworth Daniel Ayoub Mackenzie B. Mckinnon Chelsea S. Myers Tawakalit J. Busari Kyra Sabir 《Phycological Research》2023,71(3):135-139
Asterodinium gracile is a morphologically distinct, star-shaped member of the Kareniaceae with, like canonical Kareniaceae, a tertiary plastid of haptophyte origin. However, A. gracile's complement of carotenoid photosynthetic pigments has been shown to be chemotaxonomically atypical in that it possesses much less fucoxanthin when compared to that of other, canonical Kareniaceae in the genera Karenia, Karlodinium, and Takayama, also with a tertiary plastid of haptophyte origin. To date, Karenia mikimotoi, Karenia papilionacea, and Karenia selliformis are the only canonical Kareniaceae that have been shown to have a chemotaxonomically atypical carotenoid pigment composition in that they possess a gyroxanthin diester-like carotenoid not observed in other species of Karenia, Karlodinium, or Takayama (recognizing that Karenia, in general, produces fucoxanthin derivatives not observed in Karlodinium or Takayama). As a photosynthetic organism, K. mikimotoi has been shown to resemble Karenia brevis such that both species possess the chloroplast-associated galactolipids mono- and digalactosyldiacylglycerol (MGDG and DGDG, respectively) enriched with octadecapentaenoic acid (18:5(n-3)) in the sn-1 position, and hexadecenoic acid (16:0) and tetradecanoic acid (14:0) at the sn-2 position. However, K. mikimotoi is chemotaxonomically atypical beyond its carotenoid composition in that it possesses MGDG and DGDG with hexadecatetraenoic acid (16:4(n-3)), which has not been observed in any other members of the Kareniaceae, in the sn-2 position as major galactolipids. The goal of this study was to characterize the galactolipids of A. gracile with the hypothesis that they would also be atypical when compared to other canonical Kareniaceae because of A. gracile's atypical carotenoid pigment composition. To this end, we report that like K. brevis and K. mikimotoi, A. gracile produces MGDG and DGDG enriched in 18:5(n-3) at the sn-1 position and C14 fatty acids, such as 14:0, at the sn-2 position, and like K. mikimotoi, it produces 18:5(n-3)/16:4(n-3) MGDG, yet here as its most abundant galactolipid. 相似文献
12.
The glycolipid fatty acid composition of Porphyridium purpureum on a solid medium was studied in the presence of Triton X-100 (TX), sodium desocycholate (SDC), sodium dodecyl sulphate (SDS) and cetyl trimethylammonium bromide (CTAB). TLC and GC/MS were used in determining the relative fatty acid compositions of mono-and digalactosyl diglycerides (MGDG and DGDG) and in assessing the MGDG/DGDG ratio. The most common fatty acids were palmitic (16:0), stearic (18:0), linoleic (18:2 ω6), arachidonic (20:4 ω6) and eicosapentaenoic (20:5 ω3) acids, the long-chain polyunsaturated acids being more abundant in DGDG; α-linolenic acid (18:3 ω3) was absent. TX and SDC in particular caused an increase in the saturation grade of both MGDG and DGDG fatty acids at very low concentrations (5–15 ppm). With a detergent concentration of 20 ppm a reversion of this tendency was sometimes found, and the fatty acid composition approached the controls again. The effects of SDS and CTAB were not as prominent. All the detergents studied increased the normal MGDG/DGDG ratio (0.3) to a maximum of ~ 1. The effect of increasing detergent concentration is not linear. The results suggest that in some cases very low detergent concentrations can be more effective than higher ones, a fact which may be important in algae growing in polluted waters. 相似文献
13.
Amoebophrya is a parasitic, syndinian dinoflagellate genus that must infect another host dinoflagellate in order to reproduce. Work by Park et al. [Mar. Ecol. Prog. Ser., 227: 281–292 (2002)] has led to the hypothesis that Amoebophrya's development within a host cell nucleus disrupts the flow of genetic information involved in plastidial function. The possibility that genetic disruption by this parasite could lead to alterations in plastidial lipid composition during the course of an infection has not yet been elucidated. Our primary objective in this lipidomic study was to examine the chloroplast membrane galactolipid composition of Alexandrium tamarense infected by an Amoebophrya species in order to determine whether infection of A. tamarense causes a phenotypic alteration in the composition of mono- and digalactosyldiacylglycerol (MGDG and DGDG, respectively), two galactolipids that comprise the majority of photosynthetic membranes. Our secondary objective was to determine if non-photosynthetic Amoebophrya sp. either incorporated host cell MGDG and DGDG, and/or itself produced forms of MGDG and DGDG, as has been observed previously in heterotrophic apicomplexan parasites distantly related to Amoebophrya. We found that, despite development of Amoebophrya sp. within the nucleus, the composition of A. tamarense MGDG and DGDG did not change throughout the infection process. The predominant forms of these galactolipids were 18:5/18:4 (sn???1/sn?2) and 20:5/18:4 DGDG, which were present at similar abundances in both an uninfected host and a host late in the infection process just prior to release of Amoebophrya sp. dinospores. Amoebophrya sp. did not possess appreciable amounts of any forms of MGDG and DGDG. 相似文献
14.
Ryo Yamauchi Masahiro Kojima Masatoshi Isogai Koji Kato Yoshimitsu Ueno 《Bioscience, biotechnology, and biochemistry》2013,77(11):2815-2820
Spinach monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were oxidized with singlet molecular oxygen by the use of chlorophyll a as the photosensitizer. The oxidation products were separated from the unoxidized MGDG and DGDG by reverse-phase high performance liquid chromatography (HPLC). The products separated by HPLC were identified to be mono- and di-hydroperoxides formed by 1O2 oxidation of the 16:3 or 18:3 component of MGDG and DGDG. Each unsaturated fatty acid moiety in the MGDG and DGDG produced isomeric hydroperoxides in a manner similar to the corresponding fatty acid methyl ester. 相似文献
15.
Wounding stimulates the accumulation of glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid in Arabidopsis leaves
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Buseman CM Tamura P Sparks AA Baughman EJ Maatta S Zhao J Roth MR Esch SW Shah J Williams TD Welti R 《Plant physiology》2006,142(1):28-39
Although oxylipins can be synthesized from free fatty acids, recent evidence suggests that oxylipins are components of plastid-localized polar complex lipids in Arabidopsis (Arabidopsis thaliana). Using a combination of electrospray ionization (ESI) collisionally induced dissociation time-of-flight mass spectrometry (MS) to identify acyl chains, ESI triple-quadrupole (Q) MS in the precursor mode to identify the nominal masses of complex polar lipids containing each acyl chain, and ESI Q-time-of-flight MS to confirm the identifications of the complex polar lipid species, 17 species of oxylipin-containing phosphatidylglycerols, monogalactosyldiacylglycerols (MGDG), and digalactosyldiacylglycerols (DGDG) were identified. The oxylipins of these polar complex lipid species include oxophytodienoic acid (OPDA), dinor-OPDA (dnOPDA), 18-carbon ketol acids, and 16-carbon ketol acids. Using ESI triple-Q MS in the precursor mode, the accumulation of five OPDA- and/or dnOPDA-containing MGDG and two OPDA-containing DGDG species were monitored as a function of time in mechanically wounded leaves. In unwounded leaves, the levels of these oxylipin-containing complex lipid species were low, between 0.001 and 0.023 nmol/mg dry weight. However, within the first 15 min after wounding, the levels of OPDA-dnOPDA MGDG, OPDA-OPDA MGDG, and OPDA-OPDA DGDG, each containing two oxylipin chains, increased 200- to 1,000-fold. In contrast, levels of OPDA-hexadecatrienoic acid MGDG, linolenic acid (18:3)-dnOPDA MGDG, OPDA-18:3 MGDG, and OPDA-18:3 DGDG, each containing a single oxylipin chain, rose 2- to 9-fold. The rapid accumulation of high levels of galactolipid species containing OPDA-OPDA and OPDA-dnOPDA in wounded leaves is consistent with these lipids being the primary products of plastidic oxylipin biosynthesis. 相似文献
16.
17.
An exhaustive qualitative and quantitative profiling of the photosynthetic glycerolipids in three strains of the marine diatom
Skeletonema sp. was carried out by ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry.
In the diatom thylakoid membrane, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) account for about
45–70% and 5–15% of the total membrane lipids, respectively. The anionic sulfoquinovosyldiacylglycerol (SQDG) as well as the
likewise anionic phosphatidylglycerol (PG) contribute between 10–40% and 4–10% each. The predominant species of MGDG were
those with C16:3/C16:3, C20:5/16:1, and C20:5/C16:3. Three main molecular species of DGDG contained C20:5/C16:1, C20:5/C16:2, and C16:1/C16:1. The major molecular species of SQDG were those containing combinations of C14:0/C14:0, C14:0/C16:0, C14:0/C16:1, and C14:0/C16:3. All the PG classes contained the C18:1/C18:1 as the main molecular species. Based on the fatty acid species in sn-2 position, it is indicated that MGDG and DGDG are biosynthesized through prokaryotic pathway exclusively within the chloroplast,
whereas PG and SQDG have a typical mixed biosynthetic pathway (both prokaryotic pathway and eukaryotic pathways). The chemical
characteristics of photosynthetic glycerolipids related with ecological physiology are discussed. 相似文献
18.
Norihiro Sato Mikio Tsuzuki Akihiko Kawaguchi 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2003,1633(1):27-34
The fatty acid distributions at the sn-1 and sn-2 positions in major chloroplast lipids of Chlorella kessleri 11h, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), were determined to show the coexistence of both C16 and C18 acids at the sn-2 position, i.e. of prokaryotic and eukaryotic types in these galactolipids. For investigation of the biosynthetic pathway for glycerolipids in C. kessleri 11h, cells were fed with [14C]acetate for 30 min, and then the distribution of the radioactivity among glycerolipids and their constituent fatty acids during the subsequent chase period was determined. MGDG and DGDG were labeled predominantly as the sn-1-C18-sn-2-C16 (C18/C16) species as early as by the start of the chase, which suggested the synthesis of these lipids within chloroplasts via a prokaryotic pathway. On the other hand, the sn-1-C18-sn-2-C18 (C18/C18) species of these galactolipids gradually gained radioactivity at later times, concomitant with a decrease in the radioactivity of the C18/C18 species of phosphatidylcholine (PC). The change at later times can be explained by the conversion of the C18/C18 species of PC into galactolipids through a eukaryotic pathway. The results showed that C. kessleri 11h, distinct from most of other green algal species that were postulated mainly to use a prokaryotic pathway for the synthesis of chloroplast lipids, is similar to a group of higher plants designated as 16:3 plants in terms of the cooperation of prokaryotic and eukaryotic pathways to synthesize chloroplast lipids. We propose that the physiological function of the eukaryotic pathway in C. kessleri 11h is to supply chloroplast membranes with 18:3/18:3-MGDG for their functioning, and that the acquisition of a eukaryotic pathway by green algae was favorable for evolution into land plants. 相似文献
19.
A. B. Imbs I. M. Yakovleva N. A. Latyshev L. Q. Pham 《Russian Journal of Marine Biology》2010,36(6):452-457
The fatty acid (FA) composition of zooxanthellae, polyp tissue, and intact colonies was determined in soft coral Sinularia sp. and hard coral Acropora sp. Analysis of the distribution of polyunsaturated fatty acids (PUFAs) among the zooxanthellae and the host organism showed
that 18: 3n-6 and C18–22 PUFAs of the n-3 series (18: 4n-3, 20: 5n-3, 22: 5n-3, and 22: 6n-3) were mainly synthesized by the zooxanthellae and that
C20–22 PUFAs of the n-6 series (20: 3n-6, 20: 4n-6, and 22: 4n-6) were synthesized in the polyp tissue. Soft coral polyps were able
to synthesize tetracosapolyenoic FAs (24: 5n-6 and 24: 6n-3) and 18: 2n-7, their zooxanthellae synthesized C16 PUFAs (16: 2n-7, 16: 3n-4, and 16: 4n-1). It is supposed that the biosynthesis of 16: 2n-7 in Sinularia sp. and 18: 3n-6 in Acropora sp. is catalyzed by Δ6 desaturase. The relatively even distribution of three FAs (18: 2n-6, 18: 3n-6, and 16: 2n-7) among
lipids of zooxanthellae and coral polyps indicates the possible transport of these FAs between symbionts and the host organism. 相似文献
20.
The total amount of fatty acids in the mono- (MGDG) and diglycosyl diglyceride (DGDG) and more polar lipid fractions of frozen Ceratodon purpureus shoots was 4.6, 3.4 and 4.0 mg/g dry weight, respectively. The respective values for the tops of frozen Pleurozium schreberi were 2.6, 3.3 and 3.8 mg/g dry weight. The molar ratios MGDG/DGDG and MGDG + DGDG/chlorophyll were 1.3 and 3.7, respectively, for C. purpureus and 0.8 and 3.5 for P. schreberi. In C. purpureus the main fatty acids in the MGDG fraction were C 18:3ω3 (44% of the total fatty acids) and C 16:3ω3 (26%); in the DGDG fraction C 18:3ω3 (70%); and in the more polar lipid fraction C 18: 3ω3 (26%) and C 16:0 (25%). The proportion of C 20 polyunsaturated fatty acids was 15, 12 and 19% of the total fatty acids found in the MGDG, DGDG and more polar lipid fractions, respectively. In P. schreberi the proportion of C 20 polyunsaturated fatty acids was high in all polar lipid fractions (47, 42 and 25% in MGDG, DGDG and more polar lipid fractions, respectively). In addition, MGDG and DGDG fractions contained abundantly C 18:3ω3 (32 and 45%, respectively), and the more polar lipid fraction both C 18: 3ω3 (24%) and C 16:0 (27%). 相似文献