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1植物名称 岩生肥皂草(Saponaria ocymoides L.)。2材料类别茎尖和节间茎段。3培养条件 以MS为基本培养基,另加3%蔗糖和0.7%琼脂粉,pH5.85。分化、增殖培养基为:(1)MS+6-BA0.5mg.L-1(单位下同)+NAA0.1;(2)MS+6-BA1.0+NAA0.1;(3)MS+6-BA0.5+NAA0-2:(4)MS+6-BA1.0+NAA0.2。生根培养基为:(5)MS+IBA0.2;(6)MS+IBA0.4。培养温度(25+2)℃; 相似文献
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黄花小山菊的组织培养和快速繁殖 总被引:6,自引:0,他引:6
1植物名称黄花小山菊[Dendranthema hypargyrum(Diels)Ling et Shih]。
2材料类别茎尖。
3培养条件基本培养基为MS。丛生芽诱导和增殖培养基:MS+6-BA1mg.L^-1(单位下同)+NAA0.01:MS+6-BA3+NAA0.01:MS+6-BA5+NAA0.01。生根培养基:1/2MS+NAA0.01;1/2MS+NAA0.03;1/2MS+NAA0.05。 相似文献
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1植物名称本泌桉(Eucalyptus Benthamii Maiden&Cambage)。2材料类别茎段、茎尖。3培养条件以MS为基本培养基。芽诱导培养基:(1)MS+6-BA1.0mg.L-1(单位下同)+NAA0.1。增殖培养基:(2)MS+6-BA0.6+NAA0.1;(3)MS+6-BA0.2+IBA0.1。生根培养基:(4)MS+IBA0.3;(5)MS+IBA0.1。以上培养基均含30g.L-1蔗糖、6.5g·L-1琼脂,pH5.8。培养温度(25±1)℃,光照强度为40-50gm01.m-2.s-1,光照时间12h.d-1。 相似文献
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1植物名称欧洲李(Prunus domcstica L.)。2材料类别幼嫩茎段。3培养条件愈伤组织启动培养基:(1)MS+6-BA0.8mg·L^-1(单位下同)+IAA1.2;(2)MS+6-BA1.0+NAA0.1。丛生芽诱导和增殖培养基:(3)MS+6-BA0.8+IAA1.5+NAA0.05。生根培养基:(4)1/2MS+IBA0.5+根皮苷5。以上培养基除生根培养基(4)的蔗糖为15g.L^-1外,其他培养基均加入30g.L^-1蔗糖和5g·L^-1琼脂粉, 相似文献
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通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一. 相似文献
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In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980. 相似文献
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