共查询到20条相似文献,搜索用时 0 毫秒
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The regulation of the butanediol cycle in Bacillus subtilis 总被引:2,自引:0,他引:2
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The regulation of genetic competence in Bacillus subtilis 总被引:23,自引:7,他引:16
D. Dubnau 《Molecular microbiology》1991,5(1):11-18
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The NH2-terminal residues of Bacillus subtilis proteins 总被引:4,自引:0,他引:4
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The regulation of phenolic acid sysdtness in Bacillus subtilis 总被引:1,自引:0,他引:1
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The number of 5 S rRNA genes in Bacillus subtilis 总被引:9,自引:0,他引:9
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F. Marion Hulett 《Molecular microbiology》1996,19(5):933-939
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Prágai Z Allenby NE O'Connor N Dubrac S Rapoport G Msadek T Harwood CR 《Journal of bacteriology》2004,186(4):1182-1190
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Eswaramoorthy P Dravis A Devi SN Vishnoi M Dao HA Fujita M 《Journal of bacteriology》2011,193(22):6113-6122
Upon starvation, Bacillus subtilis cells switch from growth to sporulation. It is believed that the N-terminal sensor domain of the cytoplasmic histidine kinase KinA is responsible for detection of the sporulation-specific signal(s) that appears to be produced only under starvation conditions. Following the sensing of the signal, KinA triggers autophosphorylation of the catalytic histidine residue in the C-terminal domain to transmit the phosphate moiety, via phosphorelay, to the master regulator for sporulation, Spo0A. However, there is no direct evidence to support the function of the sensor domain, because the specific signal(s) has never been found. To investigate the role of the N-terminal sensor domain, we replaced the endogenous three-PAS repeat in the N-terminal domain of KinA with a two-PAS repeat derived from Escherichia coli and examined the function of the resulting chimeric protein. Despite the introduction of a foreign domain, we found that the resulting chimeric protein, in a concentration-dependent manner, triggered sporulation by activating Spo0A through phosphorelay, irrespective of nutrient availability. Further, by using chemical cross-linking, we showed that the chimeric protein exists predominantly as a tetramer, mediated by the N-terminal domain, as was found for KinA. These results suggest that tetramer formation mediated by the N-terminal domain, regardless of the origin of the protein, is important and sufficient for the kinase activity catalyzed by the C-terminal domain. Taken together with our previous observations, we propose that the primary role of the N-terminal domain of KinA is to form a functional tetramer, but not for sensing an unknown signal. 相似文献
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Kim HJ Kim SI Ratnayake-Lecamwasam M Tachikawa K Sonenshein AL Strauch M 《Journal of bacteriology》2003,185(5):1672-1680
The roles of the CcpC, CodY, and AbrB proteins in regulation of the Bacillus subtilis aconitase (citB) gene were found to be distinct and to vary with the conditions and phase of growth. CcpC, a citrate-inhibited repressor that is the primary factor regulating citB expression in minimal-glucose-glutamine medium, also contributed to repression of citB during exponential-phase growth in broth medium. A null mutation in codY had no effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mutations. However, a codY mutation slightly relieved repression during exponential growth in broth medium and completely derepressed citB expression when combined with a ccpC mutation. An abrB mutation led to decreased expression of citB during stationary phase in both broth and minimal medium. All three proteins bound in vitro to specific and partially overlapping sites within the citB regulatory region. Interaction of CcpC and CodY with the citB promoter region was partially competitive. 相似文献
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Chastukhina IB Sharipova MP Gabdrakhmanova LA Balaban NP Safina DR Kostrov SV Rudenskaia GN Leshchinskaia IB 《Mikrobiologiia》2004,73(3):335-342
The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 glutamyl endopeptidase gene on a multicopy plasmid and the effect of some nutrients on the efficiency of extracellular glutamyl endopeptidase production in the stationary growth phase were studied. In this phase, the concentration of glutamyl endopeptidase in the culture liquid peaked at the 48th and 78th h of cultivation and depended on the composition of the cultivation medium. Unlike the synthesis of glutamyl endopeptidase in the trophophase (i.e., during vegetative growth), which was suppressed by glucose, the synthesis of this enzyme during sporulation was resistant to glucose present in the cultivation medium. A multifactorial experimental design allowed optimal proportions between the concentrations of major nutrients (peptone and inorganic phosphate) to be determined. Inorganic phosphate and ammonium ions augmented the production of glutamyl endopeptidase by 30-150%, and complex organic substrates, such as casein and gelatin, enhanced the production of glutamyl endopeptidase by 50-100%. During sporulation, the production of glutamyl endopeptidase was stimulated by some bivalent cations (Ca2+, Mg2+, and Co2+) and inhibited by others (Zn2+, Fe2+, and Cu2+). The inference is drawn that the regulatory mechanisms of glutamyl endopeptidase synthesis during vegetative growth and sporulation are different. 相似文献
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Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus subtilis. 总被引:2,自引:3,他引:2 下载免费PDF全文
M LaCelle M Kumano K Kurita K Yamane P Zuber M M Nakano 《Journal of bacteriology》1996,178(13):3803-3808