共查询到20条相似文献,搜索用时 25 毫秒
1.
Watanabe T Persson P Endo H Kono M 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2000,125(1):127-136
In decapod crustaceans, deposition of calcium carbonate crystals (calcification) in the exoskeleton takes place during the postmolt phase of the molt cycle. In an attempt to identify proteins which regulate the calcification process, the differential display technique was used to identify genes which were specifically expressed in the integument during the postmolt stage in the penaeid prawn Penaeus japonicus. One of the genes thus identified, named DD9A, was expressed in the epithelial cells of the tail fan. DD9A encoded a putative precursor of a secreted protein of 113 amino acids which exhibited sequence similarities to a group of crustacean and insect cuticular proteins, suggesting that DD9A was a protein component of the exoskeleton. Another gene, DD9B, which was also transcribed specifically during the postmolt period was identified based on its sequence similarity to DD9A. Potential roles of the DD9A protein in the calcification of the exoskeleton will be discussed. 相似文献
2.
Colin W Taylor 《Cell calcium》1984,5(4):377-390
Transcellular calcium transport by the internally perfused midgut has been measured by simultaneously monitoring 45Ca removal from the perfusing saline (entry to the cells) and its appearance in the bathing saline (exit from the cells). Reduction of the Na+ gradient across the basolateral membranes of midgut epithelial cells by removal of bathing Na+ or by addition of monensin or ouabain inhibits calcium transport across the basolateral membranes. Calcium entry at the apical membranes is inhibited in parallel. The calmodulin inhibitors, trifluoperazine or calmidazolium, do not directly affect calcium transport nor do they dissociate the parallel changes in calcium entry and exit when calcium exit is inhibited. Experiments with A23187 are consistent with a role for intracellular calcium in regulating calcium entry at the apical membranes. It is suggested that calcium transport out of midgut epithelial cells is largely by Na+-Ca2+ countertransport, and that entry may be regulated by cytoplasmic calcium so that the calcium influx never exceeds the capacity of the transport mechanisms to pump it out of the cells. 相似文献
3.
The functional expression of membrane transport proteins that are responsible for exchanging sodium and protons is a ubiquitous phenomenon. Among vertebrates the Na+/H+ antiporter occurs in plasma membranes of polarized epithelial cells and non-polarized cells such as red blood cells, muscle cells, and neurons, and in each cell type the transporter exchanges one sodium for one hydrogen ion, is inhibited by amiloride, and regulates intracellular pH and sodium concentration within tight limitations. In polarized epithelial cells this transporter occurs in two isoforms, each of which is restricted to either the brush border or basolateral cell membrane, and perform somewhat different tasks in the two locations. In prokaryotic cells, sodium/proton exchange occurs by an electrogenic 1Na+/2H+ antiporter that is coupled to a primary active proton pump and together these two proteins are capable of tightly regulating the intracellular concentrations of these cations in cells that may occur in environments of 4 M NaCl or pH 10-12. Invertebrate epithelial cells from the gills, gut, and kidney also exhibit electrogenic sodium/proton exchange, but in this instance the transport stoichiometry is 2Na+/1H+. As with vertebrate electroneutral Na+/H+ exchange, the invertebrate transporter is inhibited by amiloride, but because of the occurrence of two external monovalent cation binding sites, divalent cations are able to replace external sodium and also be transported by this system. As a result, both calcium and divalent heavy metals, such as zinc and cadmium, are transported across epithelial brush border membranes in these animals and subsequently undergo a variety of biological activities once accumulated within these cells. Absorbed epithelial calcium in the crustacean hepatopancreas may participate in organismic calcium balance during the molt cycle and accumulated heavy metals may undergo complexation reactions with intracellular anions as a detoxification mechanism. Therefore, while the basic process of sodium/proton exchange may occur in invertebrate cells, the presence of the electrogenic 2Na+/1H+ antiporter in these cells allows them to perform a wide array of functions without the need to develop and express additional specialized transport proteins. J. Exp. Zool. 289:232-244, 2001. 相似文献
4.
ULTRASTRUCTURAL STUDIES OF VASOPRESSIN EFFECT ON ISOLATED PERFUSED RENAL COLLECTING TUBULES OF THE RABBIT 总被引:13,自引:6,他引:7
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Charles E. Ganote Jared J. Grantham Harold L. Moses Maurice B. Burg Jack Orloff 《The Journal of cell biology》1968,36(2):355-367
Isolated cortical collecting tubules from rabbit kidney were studied during perfusion with solutions made either isotonic or hypotonic to the external bathing medium. Examination of living tubules revealed a reversible increase in thickness of the cellular layer, prominence of lateral cell membranes, and formation of intracellular vacuoles during periods of vasopressin-induced osmotic water transport. Examination in the electron microscope revealed that vasopressin induced no changes in cell structure in collecting tubules in the absence of an osmotic difference and significant bulk water flow across the tubule wall. In contrast, tubules fixed during vasopressin-induced periods of high osmotic water transport showed prominent dilatation of lateral intercellular spaces, bulging of apical cell membranes into the tubular lumen, and formation of intracellular vacuoles. It is concluded that the ultrastructural changes are secondary to transepithelial bulk water flow and not to a direct effect of vasopressin on the cells, and that vasopressin induces osmotic flow by increasing water permeability of the luminal cell membrane. The lateral intercellular spaces may be part of the pathway for osmotically induced transepithelial bulk water flow. 相似文献
5.
Jerry R. Colca Nirmala Kotagal Paul E. Lacy Michael L. McDaniel 《生物化学与生物物理学报:生物膜》1983,729(2):176-184
The properties of active or ATP-dependent calcium transport by islet-cell endoplasmic reticulum and plasma membrane-enriched subcellular fractions were directly compared. These studies indicate that the active calcium transport systems of the two membranes are fundamentally distinct. In contrast to calcium uptake by the endoplasmic reticulum-enriched fraction, calcium uptake by islet-cell plasma membrane-enriched vesicles exhibited a different pH optimum, was not sustained by oxalate, and showed an approximate 30-fold greater affinity for ionized calcium. A similar difference in affinity for calcium was exhibited by the Ca2+-stimulated ATPase activities which are associated with these islet-cell subcellular fractions. Consistent with the effects of calmodulin on calcium transport, calmodulin stimulated Ca2+-ATPase in the plasma membranes, but did not increase calcium-stimulated ATPase activity in the endoplasmic reticulum membranes. The physiological significance of the differences observed in calcium transport by the endoplasmic reticulum and plasma membrane fractions relative to the regulation of insulin secretion by the islets of Langerhans is discussed. 相似文献
6.
Summary In an attempt to quantify possible intracellular water activity gradients during ADH-induced osmotic water flow, we employed energy dispersive X-ray microanalysis to thin, freezedried cryosections obtained from fresh, shock-frozen tissue of the toad urinary bladder. The sum of all detectable small ions (Na + K + Cl) in the cellular water space was taken as an index of the intracellular osmolarity. Presuming that all ions are osmotically active, they comprise about 90% of the cellular solutes. When the cells were exposed to dilute serosal medium, the reduction in the sum of the ions agreed well with the expected reduction in osmolarity. After inducing water flow by addition of ADH and dilution of the mucosal medium, all epithelial cells showed a fall in osmolarity. The change was more pronounced in granular cells than in basal or mitochondria-rich cells, consistent with the notion that granular cells represent the main transport pathway. Most significantly, intracellular osmolarity gradients, largely caused by an uneven distribution of K and Na, were detectable in granular cells. The gradients were not observed after ADH or mucosal dilution alone, or when the direction of transepithelial water flow was reversed. We conclude from these results that there is a significant cytoplasmic resistance to water flow which may lead to intracellular gradients of water activity. Concentration gradients of diffusible cations can be explained by a flow-induced Donnan-type distribution of fixed negative charges. With regard to transepithelial Na transport, the data suggest that ADH stimulates transport by increasing the Na permeability of the apical membranes of granular cells specifically. 相似文献
7.
An important electrophysiological variable--the transepithelial potential difference reflects the electrogenic transepithelial ion currents, which are produced and modified by ion transport processes in polarized cells of epithelium. These processes result from coordinated function of transporters in apical and basolateral cell membranes and have been observed in all epithelial tissues studied so far. The experiments were performed on isolated specimens of snail foot. In the experiments, the baseline transepithelial electrical potential difference--PD, changes of transepithelial difference during mechanical stimulation--dPD and the transepithelial resistance were measured with an Ussing apparatus. A total of 60 samples of foot ventral surface of 28 snails were studied. The transepithelial electrical potential difference of isolated foot ranged from -6.0 to 10.0 mV under different experimental conditions. Mechanical stimulation of foot ventral surface caused changes of electrogenic ion transport, observed as transient hyperpolarization (electrical potential difference became more positive). When the transepithelial electrical potential difference decreased during stimulation, the reaction was described as depolarization. When amiloride and bumetanide were added to the stimulating fluid so that the sodium and chloride ion transport pathways were inhibited, prolonged depolarization occurred. Under the influence of different stimuli: mechanical (gentle rinsing), chemical (changes of ion concentrations) and pharmacological (application of ion inhibitors), transient changes of potential difference (dPD) were evoked, ranging from about -0.7 to almost 2.0 mV. Changes in transepithelial potential difference of the pedal surface of the snail's foot related to these physiological stimuli are probably involved in the locomotion of the animal and are under control of the part of the nervous system in which tachykinin related peptides (TRP) act as transmitters. 相似文献
8.
Properties of a polarized primary culture from rat renal inner medullary collecting duct (IMCD) cells 总被引:1,自引:0,他引:1
Birgit Ruhfus H. Günther Bauernschmitt Rolf K. H. Kinne 《In vitro cellular & developmental biology. Animal》1998,34(3):227-231
Summary A primary culture from rat renal IMCD cells was established to investigate the permeability characteristics of the luminal
and contraluminal plasma membranes of the papillary collecting duct in vitro. Freshly isolated IMCD cells were grown on filters in a special “epithelial cell” medium. Confluency was proved with an epithelial
volt/ohm meter. After 7 d of culture the transepithelial resistance reached more than 1000 Ω×cm2. A polarization of the cells with regard to a basolateral localization of a lactate efflux system, and an l-alanine transport system was achieved. The hypotonicity-activated release systems for the organic osmolytes sorbitol and
betaine were also located basolaterally, whereas taurine, glycerophosphorylcholine, and myo-inositol left the cells at both
cell poles but with different capacity. Morphological observations revealed also that the monolayer was well differentiated.
Thus, a model of a renal collecting duct epithelium was established which can be used to analyze polarized and differentiated
transport processes across the epithelial cells and their plasma membranes. 相似文献
9.
PDZ proteins retain and regulate membrane transporters in polarized epithelial cell membranes 总被引:9,自引:0,他引:9
PDZ proteins retain and regulate membrane transporters in polarized epithelial cell membranes. Am J Physiol Cell Physiol 288: C20C29, 2005; doi:10.1152/ajpcell.00368.2004.The plasma membrane of epithelial cells is subdivided into two physically separated compartments known as the apical and basolateral membranes. To obtain directional transepithelial solute transport, membrane transporters (i.e., ion channels, cotransporters, exchangers, and ion pumps) need to be targeted selectively to either of these membrane domains. In addition, the transport properties of an epithelial cell will be maintained only if these membrane transporters are retained and properly regulated in their specific membrane compartments. Recent reports have indicated that PDZ domain-containing proteins play a dual role in these processes and, in addition, that different apical and basolateral PDZ proteins perform similar tasks in their respective membrane domains. First, although PDZ-based interactions are dispensable for the biosynthetic targeting to the proper membrane domain, the PDZ network ensures that the membrane proteins are efficiently retained at the cell surface. Second, the close spatial positioning of functionally related proteins (e.g., receptors, kinases, channels) into a signal transduction complex (transducisome) allows fast and efficient control of membrane transport processes. retention of apical and basolateral membrane proteins; transducisomes; protein complex formation 相似文献
10.
Yongping Gao Christopher M. Gillen Michele G. Wheatly 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2009,152(3):216-225
Calmodulin (CaM) is a highly conserved calcium (Ca2+) binding protein that transduces Ca2+ signals into downstream effects influencing a range of cellular processes, including Ca2+ homeostasis. The present study explores CaM expression when Ca2+ homeostasis is challenged during the mineralization cycle of the freshwater crayfish (Procambarus clarkii). In this paper we report the cloning of a CaM gene from axial abdominal crayfish muscle (referred to as pcCaM). The pcCaM mRNA is ubiquitously expressed but is far more abundant in excitable tissue (muscle, nerve) than in any epithelia (gill, antennal gland, digestive) suggesting that it plays a greater role in the biology of excitation than in epithelial ion transport. In muscle cells the pcCaM was colocalized on the plasma membrane with the Ca2+ ATPase (PMCA) known to regulate intracellular Ca2+ through basolateral efflux. While PMCA exhibits a greater upregulation in epithelia (than in non-epithelial tissues) during molting stages requiring transcellular Ca2+ flux (pre- and postmolt compared with intermolt), expression of pcCaM exhibited a uniform increase in epithelial and non-epithelial tissues alike. The common increase in expression of CaM in all tissues during pre- and postmolt stages (compared with intermolt) suggests that the upregulation is systemically (hormonally) mediated. Colocalization of CaM with PMCA confirms physiological findings that their regulation is linked. 相似文献
11.
Summary A modified cytochemical technique with 5-adenylylimidodiphosphate as substrate, was used to examine the distribution of adenylate cyclase in cells comprising the transepithelial Na+ transport pathway in isolated frog skin epithelium. Particular attention was paid to the effects of fixation on the activity and localization of adenylate cyclase. Fixation in glutaraldehyde alone or in combination with paraformaldehyde reduced the amount of reaction product, while better results were obtained using unfixed tissues. Optimum results were obtained following stimulation of adenylate cyclase with forskolin and in the presence of specific metabolic inhibitors. Adenylate cyclase was localized in the basolateral membranes of the principal cells which constitute a functional syncytium for Na+ transport and was absent from the apical membranes of the outermost granulosum cells. This distribution is consistent with the transepithelial Na+ transport model and defines the functional morphology of the cells involved in Na+ transport across frog skin. The results are compatible with the process of Na+ re-absorption across other epithelial cells, verifying that frog skin is a convenient model-tissue to study Na+ transport mechanisms. Adenylate cyclase was also found in membranes of the mitochondria-rich cells, a minor and parallel Na+ transporting pathway. 相似文献
12.
M. G. Sá G. A. Ahearn F. P. Zanotto 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2009,179(5):605-615
Gills are the first site of impact by metal ions in contaminated waters. Work on whole gill cells and metal uptake has not
been reported before in crustaceans. In this study, gill filaments of the American lobster, Homarus americanus, were dissociated in physiological saline and separated into several cell types on a 30, 40, 50, and 80% sucrose gradient.
Cells from each sucrose solution were separately resuspended in physiological saline and incubated in 65Zn2+ in order to assess the nature of metal uptake by each cell type. Characteristics of zinc accumulation by each kind of cell
were investigated in the presence and absence of 10 mM calcium, variable NaCl concentrations and pH values, and 100 μM verapamil,
nifedipine, and the calcium ionophore A23187. 65Zn2+ influxes were hyperbolic functions of zinc concentration (1–1,000 μM) and followed Michaelis–Menten kinetics. Calcium reduced
both apparent zinc binding affinity (K
m) and maximal transport velocity (J
max) for 30% sucrose cells, but doubled the apparent maximal transport velocity for 80% sucrose cells. Results suggest that calcium,
sodium, and protons enter gill epithelial cells by an endogenous broad-specificity cation channel and trans-stimulate metal
uptake by a plasma membrane carrier system. Differences in zinc transport observed between gill epithelial cell types appear
related to apparent affinity differences of the transporters in each kind of cell. Low affinity cells from 30% sucrose were
inhibited by calcium, while high affinity cells from 80% sucrose were stimulated. 65Zn2+ transport was also studied by isolated, intact, gill filament tips. These intact gill fragments generally displayed the same
transport properties as did cells from 80% sucrose and provided support for metal uptake processes being an apical phenomenon.
A working model for zinc transport by lobster gill cells is presented. 相似文献
13.
Calcium phosphate deposits in domes of human pancreatic adenocarcinoma (Capan-1) cell cultures 总被引:2,自引:0,他引:2
E Hollande J H Levrat di Donato M Fanjul C Palevody M Daumas J Puech G Ratovo 《Biology of the cell / under the auspices of the European Cell Biology Organization》1990,69(3):191-203
Human pancreatic cells of the Capan-1 line form domes in culture during the stationary growth stage. The domes are thought to be a result of the transport of water and electrolytes by the Capan-1 cells. In older Capan-1 cultures, the epithelial sheets formed thickenings from several layers of cells of which the outermost ones were joined by tight type junctions. In the intracellular space, deposits of insoluble calcium salts were observed. Culture of Capan-1 cells in the presence of fibroblasts prolonged survival of the cultures with intact domes for more than 80 days. The Capan-1 cells proliferated forming multilayers and closed cavities which we called super-domes. X-ray spectrometry and electron diffraction analysis showed that the abundant deposits inside these cavities consisted of calcium phosphate in an apatite structure. The number of these deposits increased with time in culture, and they appeared to be formed at the sites of contact with an extracellular matrix consisting of cell debris. Deposits were not observed within the culture medium. Cells from domes were stained cytochemically for ATPases and alkaline phosphatases and examined by light and electron microscopy. The Capan-1 cells surrounding the domes were differentiated, polarized cells containing placental type alkaline phosphatases on their apical membranes and Ca2(+)-ATPases on their basolateral membranes. These enzymes were thought to play a role in the accumulation of phosphate and Ca2+ ions in the dome cavities, which then formed crystals in the presence of organic compounds produced by lysis of cells of the deepest layers of the super-domes. The crystals of hydroxyapatite observed in standard Capan-1 cell cultures and those cocultured with fibroblasts were assumed to be a result of transepithelial transport of Ca2+ and phosphate ions by these cells. 相似文献
14.
Rabbani I Siegling-Vlitakis C Noci B Martens H 《American journal of physiology. Regulatory, integrative and comparative physiology》2011,301(2):R313-R319
Na absorption across the cornified, multilayered, and squamous rumen epithelium is mediated by electrogenic amiloride-insensitive transport and by electroneutral Na transport. High concentrations of amiloride (>100 μM) inhibit Na transport, indicating Na(+)/H(+) exchange (NHE) activity. The underlying NHE isoform for transepithelial Na absorption was characterized by mucosal application of the specific inhibitor HOE642 for NHE1 and S3226 for NHE3 in Ussing chamber studies with isolated epithelia from bovine and sheep forestomach. S3226 (1 μM; NHE3 inhibitor) abolished electroneutral Na transport under control conditions and also the short-chain fatty acid-induced increase of Na transport via NHE. However, HOE642 (30 μM; NHE1 inhibitor) did not change Na transport rates. NHE3 was immunohistochemically localized in membranes of the upper layers toward the lumen. Expression of NHE1 and NHE3 has been previously demonstrated by RT-PCR, and earlier experiments with isolated rumen epithelial cells have shown the activity of both NHE1 and NHE3. Obviously, both isoforms are involved in the regulation of intracellular pH, pH(i). However, transepithelial Na transport is only mediated by apical uptake via NHE3 in connection with extrusion of Na by the basolaterally located Na-K-ATPase. The missing involvement of NHE1 in transepithelial Na transport suggests that the proposed "job sharing" in epithelia between these two isoforms probably also applies to forestomach epithelia: NHE3 for transepithelial transport and NHE1 for, among others, pH(i) and volume regulation. 相似文献
15.
Vacuolar ion channel of the yeast, Saccharomyces cerevisiae 总被引:6,自引:0,他引:6
Y Wada Y Ohsumi M Tanifuji M Kasai Y Anraku 《The Journal of biological chemistry》1987,262(36):17260-17263
Ionic flux is most likely to regulate the chemiosmotic potential differences across vacuolysosomal membranes in animal, plant, and fungal cells. We found a membrane potential-dependent cation channel in yeast vacuolar membrane and characterized its several features by an electrophysiological method using artificial planar bilayer membranes incorporated with isolated yeast vacuolar membrane vesicles. This ion channel conducts K+ (single channel conductance, 435 pS in 0.3 M KCl) and several other monovalent cations (Cs+, Na+, and Li+) with broad selectivity, but does not conduct Cl-. The opening of this channel is regulated by the membrane potential and the presence of calcium ion on the cytoplasmic face. These characteristics suggested that the vacuolar cation channel functions as one of essential components for formation and regulation of the chemical and electrical potential differences across the vacuolar membrane. 相似文献
16.
Pulsed magnetic fields (PMFs) are widely used to treat difficult fractures of bone and other disorders of connective tissue. It is not clear how they interact with tissue metabolism, although it has been proposed that induced currents or electric fields impinging on cell membranes may modify their ion transport function. This hypothesis was tested by treating in vitro models for ion transport processes with short-term exposure to PMFs. No change occurred in active transport of potassium or calcium in human red cells or in calcium transport through an epithelial membrane. We considered less direct action on red cell membranes, that their permeability might be modified after PMF treatment, and also that PMFs might alter the extracellular ionic activity within connective tissue by interacting with its Donnan potential. Each of these studies proved negative, and we conclude that the PMF waveforms used here do not exert a general short-term effect on cellular ion transport. 相似文献
17.
Ion channels in renal epithelia are involved in maintenance of the volume and ion composition of the epithelial cells themselves and of the entire organism. The latter function depends on transepithelial ion transport, a process that often involves ion channels at the apical (luminal) and/or the basolateral (contraluminal) cell membranes. Regulation of these channels is accomplished within many different time frames, each of which can involve different molecular mechanisms of regulation. Changes in membrane voltage, intracellular ion composition, or mechanical force on the membrane mediate short-term regulation. Biosynthesis, degradation, and reversible transfer of channels to or from cytoplasmic stores are responsible for longer term regulation. Covalent modification of channel proteins can be involved in either short- or long-term regulation. In this review we outline the different models of ion channel regulation in renal epithelia and give examples that emphasize the physiological roles of these channels in specific nephron segments. 相似文献
18.
Electrolyte absorption by gallbladders: models of transport. 总被引:1,自引:0,他引:1
R C Rose 《Life sciences》1978,23(15):1517-1531
A model of electrolyte absorption by gallbladder epithelium has been presented previously on the basis of studies on gallbladders of 12 species, including fishes, frogs, toad, turtle, guinea pig, rabbit, cat and dog. This model incorporates several physiologic and morphologic characteristics common to other transporting epithelia (e.g., intestine) such as energy-dependent solute pumps, osmotically-induced water flow into the lateral intercellular space and bulk flow of fluid driven by hydrostatic pressure along the lateral space toward the basement membrane. Because the transepithelial PD across the gallbladders of each of these species was near zero under most experimental conditions, the active transport mechanism (or, “pump”) at the basolateral membrane was considered to move Na and Cl in a coupled, one-for-one manner. The carrier mechanism was postulated to have a binding site for Na and one for Cl; it would function only if both sites were filled.Gallbladders from six other species investigated more recently (including man, monkeys, goose and Necturus) have serosa-positive transepithelial PDs of 2–8 mV. The possibility was suggested that rheogenic Na transport from mucosa to serosa might account for the PD in this group of tissues and the original model of transport would be inappropriate. This review will explore the possibility that a single model of electrolyte transport accounts for the data collected on gallbladders with PDs near zero and those having significant transepithelial PDs.An important finding which helps to reconcile the experimental observations on the two groups of gallbladders was the demonstration of coupled flux of Na and Cl from the mucosal solution into the epithelial cells. It appears that this rigid coupling of Na and Cl influx accounts for the lack of a significant PD in gallbladders of those species investigated in the earlier studies, and that rheogenic Na transport may be a property common to gallbladders of all species. 相似文献
19.
CELLULAR MECHANISM OF INTESTINAL PERMEABILITY ALTERATIONS PRODUCED BY CHELATION DEPLETION 总被引:5,自引:2,他引:3
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The absorption of phenolsulfonphthalein (phenol red) was used as a measure in vivo of intestinal permeability in anesthetized rats. A chelating agent, sodium ethylenediaminetetraacetate (NaEDTA), placed in the lumen evoked a fivefold increase in membrane permeability; at the same time the mucosal content of magnesium and calcium decreased significantly. Making either magnesium or calcium available to the luminal surface of the membrane in isotonic solution restored normal permeability and brought the cation contents above the original levels. Electron micrographs of tissues treated in vivo with NaEDTA revealed (a) rounded swellings on the microvilli in the area of the junctional complexes between adjacent epithelial cells, (b) widening of intercellular channels particularly in the region of the intermediate junctions (zonulae adhaerentes), and (c) loss of architectural detail in the region of the desmosomes (maculae adhaerentes) with separation of their dense borders. All of these alterations in fine structure could be reversed by in vivo cation replacements which reinstated normal permeability. The implications of these findings on mechanisms of fluid transport across epithelial membranes are discussed, and a working hypothesis for the role of divalent cations in membrane permeability regulation is presented. 相似文献
20.
Jocelyne Enouf Raymonde Bredoux Bernadette Boizard Jean-Luc Wautier Hugues Chap Jany Thomas Meno de Metz Sylviane Levy-Toledano 《Biochemical and biophysical research communications》1984,123(1):50-58
Simultaneous isolation of two platelet membrane subfractions was achieved by centrifugation on 40 % sucrose from a 100.000g crude membrane fraction. Characterization of both types of membranes was carried out by different biochemical and immunological markers. Using a surface label, 3H Concanavaline A (3HCon A), a marker enzyme, phosphodiesterase, and lipid analysis, one of the fraction has been identified as external or plasma membranes, the other consists of intracellular membranes. Further two specific antibodies directed against external membrane antigens (LeKa and LgG L) react almost exclusively with the external membranes. Finally both kinds of membranes were able to uptake calcium but the affinity for this cation was higher for the internal than for the external membranes. This suggests that both membranes are implicated in the regulation of the cytoplasmic calcium concentration and that the internal membranes (dense tubular system) play the major part in this regulation. 相似文献