首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 31 毫秒
1.
In this study,we determined the contractile properties of single chemically skinnedfibers prepared from the medial gastrocnemius (MG) and soleus (Sol)muscles of adult male rhesus monkeys and assessed the effects of thespaceflight living facility known as the experiment support primatefacility (ESOP). Muscle biopsies were obtained 4 wk before andimmediately after an 18-day ESOP sit, and fiber type was determined byimmunohistochemical techniques. The MG slow type I fiber wassignificantly smaller than the MG type II, Sol type I, and Sol type IIfibers. The ESOP sit caused a significant reduction in the diameter oftype I and type I/II (hybrid) fibers of Sol and MG type II and hybridfibers but no shift in fiber type distribution. Single-fiber peak force(mN and kN/m2) was similarbetween fiber types and was not significantly different from valuespreviously reported for other species. The ESOP sit significantlyreduced the force (mN) of Sol type I and MG type II fibers. Thisdecline was entirely explained by the atrophy of these fiber typesbecause the force per cross-sectional area (kN/m2) was not altered. Peakpower of Sol and MG fast type II fiber was 5 and 8.5 times that of slowtype I fiber, respectively. The ESOP sit reduced peak power by 25 and18% in Sol type I and MG type II fibers, respectively, and, for theformer fiber type, shifted the force-pCa relationship to the right,increasing the Ca2+ activationthreshold and the free Ca2+concentration, eliciting half-maximal activation. The ESOP sit had noeffect on the maximal shortening velocity(Vo) of anyfiber type. Vo ofthe hybrid fibers was only slightly higher than that of slow type Ifibers. This result supports the hypothesis that in hybrid fibers theslow myosin heavy chain would be expected to have a disproportionatelygreater influence onVo.

  相似文献   

2.
Thompson, L. V., and J. A. Shoeman. Contractilefunction of single muscle fibers after hindlimb unweighting in aged rats. J. Appl. Physiol. 84(1):229-235, 1998.This investigation determined how muscle atrophyproduced by hindlimb unweighting (HU) alters the contractile functionof single muscle fibers from older animals (30 mo). After 1 wk of HU,small bundles of fibers were isolated from the soleus muscles and thedeep region of the lateral head of the gastrocnemius muscles. Singleglycerinated fibers were suspended between a motor lever and forcetransducer, functional properties were studied, and the myosin heavychain (MHC) composition was determined electrophoretically. After HU, the diameter of type I MHC fibers of the soleus declined (88 ± 2 vs. 80 ± 4 µm) and reductions were observed in peak active force (47 ± 3 vs. 28 ± 3 mg) and peak specific tension(Po; 80 ± 5 vs. 56 ± 5 kN/m2). The maximal unloadedshortening velocity increased. The type I MHC fibers from thegastrocnemius showed reductions in diameter (14%), peak active force(41%), and Po (24%), whereas thetype IIa MHC fibers showed reductions in peak active force andPo. Thus 1 wk ofinactivity has a significant effect on the force-generating capacity ofsingle skeletal muscle fibers from older animals in a fibertype-specific manner (type I MHC > type IIa MHC > type I-IIa MHC).The decline in the functional properties of single skeletal musclefibers in the older animals appears to be more pronounced than what hasbeen reported in younger animal populations.

  相似文献   

3.
Electrophoretic analyses of muscle proteins in whole musclehomogenates and single muscle fiber segments were used to examine myosin heavy chain (MHC) and myosin light chain 2 (MLC2) isoform composition and fiber type populations in soleus muscles from spontaneously hypertensive rats (SHRs) and their age-matchednormotensive controls [Wistar-Kyoto (WKY) rats], at threestages in the development of high blood pressure (4 wk, 16 wk, and 24 wk of age). Demembranated (chemically skinned with 2% Triton X-100),single fiber preparations were used to determine the maximumCa2+-activated force percross-sectional area, calcium sensitivity, and degree of cooperativityof the contractile apparatus andCa2+-regulatory system withrespect to Ca2+. The results showthat, at all ages examined, 1) SHRsoleus contained a lower proportion of MHCI and MLC2 slow (MLC2s) and ahigher proportion of MHCIIa, MHCIId/x, and MLC2 fast (MLC2f )isoforms than the age-matched controls;2) random dissection of single fibers from SHR and WKY soleus produced four populations of fibers: type I (expressing MHCI), type IIA (expressing MHCIIa), hybrid typeI+IIA (coexpressing MHCI and MHCIIa), and hybrid type IIA+IID (coexpressing MHCIIa and MHCIId/x); and3) single fiber dissection from SHRsoleus yielded a lower proportion of type I fibers, a higher proportionof fast-twitch fibers (types IIA and IIA+IID), and a higher proportionof hybrid fibers (types I+IIA and IIA+IID) than the homologous musclesfrom the age-matched WKY rats. Because the presence of hybrid fibers isviewed as a marker of muscle transformation, these data suggest thatSHR soleus undergoes transformation well into adulthood. Our data showalso that, for a given fiber type, there are no significant differencesbetween SHR and WKY soleus muscles with respect to any of theCa2+-activation propertiesexamined. This finding indicates that the lower specific tensionsreported in the literature for SHR soleus muscles are not due tostrain- or hypertension-related differences in the function of thecontractile apparatus or regulatory system.  相似文献   

4.
Soleus musclefibers from the rat display a reduction in peak power andCa2+ sensitivity after hindlimbsuspension. To examine human responses to non-weight bearing, weobtained soleus biopsies from eight adult men before and immediatelyafter 17 days of bed rest (BR). Single chemically skinned fibers weremounted between a force transducer and a servo-controlled positionmotor and activated with maximal (isotonic properties) and/orsubmaximal (Ca2+ sensitivity)levels of free Ca2+. Gelelectrophoresis indicated that all pre- and post-BR fibers expressedtype I myosin heavy chain. Post-BR fibers obtained from one subjectdisplayed increases in peak power andCa2+ sensitivity. In contrast,post-BR fibers obtained from the seven remaining subjects showed anaverage 11% reduction in peak power (P < 0.05), with each individualdisplaying a 7-27% reduction in this variable. Post-BR fibersfrom these subjects were smaller in diameter and produced 21% lessforce at the shortening velocity associated with peak power. However,the shortening velocity at peak power output was elevated 13% in thepost-BR fibers, which partially compensated for their lower force.Post-BR fibers from these same seven subjects also displayed a reducedsensitivity to free Ca2+(P < 0.05). These results indicatethat the reduced functional capacity of human lower limb extensormuscles after BR may be in part caused by alterations in thecross-bridge mechanisms of contraction.

  相似文献   

5.
Using a single, mechanically skinned fiber approach, we tested the hypothesis that denervation (0 to 50 days) of skeletal muscles that do not overlap in fiber type composition [extensor digitorum longus (EDL) and soleus (SOL) muscles of Long-Evans hooded rats] leads to development of different fiber phenotypes. Denervation (50 day) was accompanied by 1) a marked increase in the proportion of hybrid IIB/D fibers (EDL) and I/IIA fibers (SOL) from 30% to >75% in both muscles, and a corresponding decrease in the proportion of pure fibers expressing only one myosin heavy chain (MHC) isoform; 2) complex muscle- and fiber-type specific changes in sarcoplasmic reticulum Ca2+-loading level at physiological pCa 7.1, with EDL fibers displaying more consistent changes than SOL fibers; 3) decrease by 50% in specific force of all fiber types; 4) decrease in sensitivity to Ca2+, particularly for SOL fibers (by 40%); 5) decrease in the maximum steepness of the force-pCa curves, particularly for the hybrid I/IIA SOL fibers (by 35%); and 6) increased occurrence of biphasic behavior with respect to Sr2+ activation in SOL fibers, indicating the presence of both slow and fast troponin C isoforms. No fiber types common to the two muscles were detected at any time points (day 7, 21, and 50) after denervation. The results provide strong evidence that not only neural factors, but also the intrinsic properties of a muscle fiber, influence the structural and functional properties of a particular muscle cell and explain important functional changes induced by denervation at both whole muscle and single cell levels. mechanically skinned fibers; myosin heavy chain isoforms; lineage; sarcoplasmic reticulum; Ca2+; Sr2+ sensitivity; Long-Evans hooded rat  相似文献   

6.
This investigation compared how hindlimbunweighting (HU) affected the contractile function of single soleusmuscle fibers from 12- and 30-mo-old Fischer 344 Brown Norway F1 Hybridrats. After 1 wk of HU, functional properties of singlepermeabilized fibers were studied, and, subsequently, the fiber typewas established by myosin heavy chain (MHC) analysis. After HU, therelative mass of soleus declined by 12 and 19% and the relative massof the gastrocnemius declined by 15 and 13% in 12- and 30-mo-oldanimals, respectively. In 12-mo-old animals, the peak active force(5.0 ± 0.2 ×104 vs. 3.8 ± 0.2 ×104 N) and thepeak specific tension (92 ± 4 vs. 78 ± 3 kN/m2) were significantlyreduced in the MHC type I fibers by 24 and 15%, respectively. In30-mo-old animals, the peak active force declined by 40% (4.7 ± 0.2 ×104vs. 2.8 ± 0. 3 ×104 N) and the peakspecific tension declined by 30% (79 ± 5 vs. 55 ± 4 kN/m2). The maximal unloadedshortening velocity of the MHC type I fibers increased in 12-mo-oldanimals (from 1.65 ± 0.12 to 2.59 ± 0.26 fiber lengths/s) andin 30-mo-old animals (from 0.90 ± 0.09 to 1.50 ± 0.10 fiberlengths/s) after HU. Collectively, these data suggest thatthe effects of HU on single soleus skeletal muscle fiber function occurin both age groups; however, the single MHC type I fibers from theolder animals show greater changes than do single MHC type I fibersfrom younger animals.

  相似文献   

7.
Hunter, Kam D., and John A. Faulkner. Pliometriccontraction-induced injury of mouse skeletal muscle: effect of initial length. J. Appl. Physiol. 82(1):278-283, 1997.For single pliometric (lengthening) contractionsinitiated from optimal fiber length (Lf), the mostimportant factor determining the subsequent force deficit is the workinput during the stretch. We tested the hypothesis that regardless ofthe initial length, the force deficit is primarily a function of thework input. Extensor digitorum longus muscles of mice were maximallyactivated in situ and lengthened at 2 Lf /s from oneof three initial fiber lengths (90, 100, or 120% of Lf) to one ofthree final fiber lengths (150, 160, or 170% of Lf). Maximalisometric force production was assessed before and after the pliometriccontraction. No single mechanical factor, including thework input(r2 = 0.34), was sufficient to explain the differences in force deficits observed among groups. Therefore, the force deficit appears to arisefrom a complex interaction of mechanicalevents. With the data grouped by initial fiber length,the correlation between the average work and the average force deficitwas high(r2 = 0.97-0.99). Consequently, differences in force deficits among groups were best explained on the basis of the initial fiber length andthe work input during the stretch.

  相似文献   

8.
Lynch, Gordon S., Alan Hayes, Siun P. Campbell, and David A. Williams. Effects of2-agonist administration andexercise on contractile activation of skeletal muscle fibers.J. Appl. Physiol. 81(4):1610-1618, 1996.Clenbuterol, a2-adrenoceptor agonist, hastherapeutic potential for the treatment of muscle-wasting diseases, yetits effects, especially at the single-fiber level, have not been fullycharacterized. Male C57BL/10 mice were allocated to three groups:Control-Treated mice were administered clenbuterol (2 mg · kg1 · day1)via their drinking water for 15 wk; Trained-Treated mice underwent low-intensity training (unweighted swimming, 5 days/wk, 1 h/day) inaddition to receiving clenbuterol; and Control mice were sedentary anduntreated. Contractile characteristics were determined on membrane-permeabilized fibers from the extensor digitorum longus (EDL)and soleus muscles. Fast fibers from the EDL and soleus muscles ofTreated mice exhibited decreases inCa2+ sensitivity. Enduranceexercise offset clenbuterol's effects, demonstrated by similarCa2+ sensitivities in theTrained-Treated and Control groups. Long-term clenbuterol treatment didnot affect the normalized maximal tension of fast or slow fibers butincreased the proportion of fast fibers in the soleus muscle. Trainingincreased the proportion of fibers with high and intermediate succinatedehydrogenase activity in the EDL and soleus muscles, respectively. Ifclenbuterol is to be used for treating muscle-wasting disorders, someform of low-intensity exercise might be encouraged such thatpotentially deleterious slow-to-fast fiber type transformations areminimized. Indeed, in the mouse, low-intensity exercise appears toprevent these effects.

  相似文献   

9.
Talmadge, Robert J., Roland R. Roy, and V. Reggie Edgerton.Distribution of myosin heavy chain isoforms in non-weight-bearing rat soleus muscle fibers. J. Appl.Physiol. 81(6): 2540-2546, 1996.The effects of14 days of spaceflight (SF) or hindlimb suspension (HS) (Cosmos 2044)on myosin heavy chain (MHC) isoform content of the rat soleus muscleand single muscle fibers were determined. On the basis ofelectrophoretic analyses, there was a de novo synthesis of type IIx MHCbut no change in either type I or IIa MHC isoform proportions aftereither SF or HS compared with controls. The percentage of fiberscontaining only type I MHC decreased by 26 and 23%, and the percentageof fibers with multiple MHCs increased from 6% in controls to 32% inHS and 34% in SF rats. Type IIx MHC was always found in combinationwith another MHC or combination of MHCs; i.e., no fibers contained typeIIx MHC exclusively. These data suggest that the expression of thenormal complement of MHC isoforms in the adult rat soleus muscle isdependent, in part, on normal weight bearing and that the absence ofweight bearing induces a shift toward type IIx MHC protein expression in the preexisting type I and IIa fibers of the soleus.

  相似文献   

10.
Lewis, Michael I., Thomas J. LoRusso, and Mario Fournier.Effect of insulin-like growth factor I and/or growthhormone on diaphragm of malnourished adolescent rats.J. Appl. Physiol. 82(4):1064-1070, 1997.Young growing animals appear to havesignificantly reduced "nutritional reserve" to short periods ofunstressed starvation compared with adults, with resultant growtharrest and/or atrophy of diaphragm (Dia) muscle fibers. The aimof this study was to assess in an adolescent rat model of acutenutritional deprivation (ND; 72 h) the impact of insulin-like growthfactor I (IGF-I), with or without added growth hormone (GH), on thecross-sectional areas (CSA) of individual Dia muscle fibers. Fivegroups were studied: 1) control(Ctr); 2) ND;3) ND given IGF-I (ND/IGF-I); 4) ND given GH (ND/GH); and5) ND given a combination of IGF-I and GH (ND/IGF-I/GH). IGF-I was given by a subcutaneously implanted osmotic minipump (200 µg/day), whereas GH was administered twice daily by a subcutaneous injection (250 µg every 12 h). Isometric contractile and fatigue properties of the Dia were determined in vitro.Forces were normalized for muscle CSA (i.e., specific force). Dia fibertype proportions were determined histochemically, and fiber CSA wasquantified by using a computer-based image-processing system. Totalserum IGF-I concentrations were significantly reduced in ND and ND/GHanimals, compared with Ctr, and elevated in the groups receiving IGF-I.The provision of growth factors did not alter the contractile orfatigue properties of ND animals. Dia fiber type proportions weresimilar among the groups. In ND animals, there was a significantreduction in the CSA of types I, IIa, IIx, and IIc Dia fibers comparedwith Ctr. The administration of IGF-I alone or in combination with GHto ND animals significantly diminished the reduction in Dia fiber size.GH alone had no effect on Dia fiber size in ND animals. We concludethat with acute ND the peripheral resistance to the action of GHappears to be bypassed by the administration of IGF-I alone or incombination with GH.

  相似文献   

11.
McCall, G. E., W. C. Byrnes, A. Dickinson, P. M. Pattany,and S. J. Fleck. Muscle fiber hypertrophy, hyperplasia, and capillary density in college men after resistance training.J. Appl. Physiol. 81(5):2004-2012, 1996.Twelve male subjects with recreationalresistance training backgrounds completed 12 wk of intensifiedresistance training (3 sessions/wk; 8 exercises/session; 3 sets/exercise; 10 repetitions maximum/set). All major muscle groupswere trained, with four exercises emphasizing the forearm flexors.After training, strength (1-repetition maximum preacher curl) increasedby 25% (P < 0.05). Magneticresonance imaging scans revealed an increase in the biceps brachiimuscle cross-sectional area (CSA) (from 11.8 ± 2.7 to 13.3 ± 2.6 cm2;n = 8;P < 0.05). Muscle biopsies of thebiceps brachii revealed increases(P < 0.05) in fiber areas for type I(from 4,196 ± 859 to 4,617 ± 1,116 µm2;n = 11) and II fibers (from 6,378 ± 1,552 to 7,474 ± 2,017 µm2;n = 11). Fiber number estimated fromthe above measurements did not change after training (293.2 ± 61.5 × 103 pretraining; 297.5 ± 69.5 × 103 posttraining;n = 8). However, the magnitude ofmuscle fiber hypertrophy may influence this response because thosesubjects with less relative muscle fiber hypertrophy, but similarincreases in muscle CSA, showed evidence of an increase in fibernumber. Capillaries per fiber increased significantly(P < 0.05) for both type I(from 4.9 ± 0.6 to 5.5 ± 0.7;n = 10) and II fibers (from 5.1 ± 0.8 to 6.2 ± 0.7; n = 10). Nochanges occurred in capillaries per fiber area or muscle area. Inconclusion, resistance training resulted in hypertrophy of the totalmuscle CSA and fiber areas with no change in estimated fiber number,whereas capillary changes were proportional to muscle fiber growth.

  相似文献   

12.
Delp, Michael D., Changping Duan, John P. Mattson, andTimothy I. Musch. Changes in skeletal muscle biochemistry and histology relative to fiber type in rats with heart failure.J. Appl. Physiol. 83(4):1291-1299, 1997.One of the primary consequences of leftventricular dysfunction (LVD) after myocardial infarction is adecrement in exercise capacity. Several factors have been hypothesizedto account for this decrement, including alterations in skeletal musclemetabolism and aerobic capacity. The purpose of this study was todetermine whether LVD-induced alterations in skeletal muscle enzymeactivities, fiber composition, and fiber size are1) generalized in muscles orspecific to muscles composed primarily of a given fiber type and2) related to the severity of theLVD. Female Wistar rats were divided into three groups: sham-operatedcontrols (n = 13) and rats withmoderate (n = 10) and severe(n = 7) LVD. LVD was surgicallyinduced by ligating the left main coronary artery and resulted inelevations (P < 0.05) in leftventricular end-diastolic pressure (sham, 5 ± 1 mmHg; moderate LVD,11 ± 1 mmHg; severe LVD, 25 ± 1 mmHg). Moderate LVDdecreased the activities of phosphofructokinase (PFK) and citratesynthase in one muscle composed of type IIB fibers but did not modifyfiber composition or size of any muscle studied. However, severe LVDdiminished the activity of enzymes involved in terminal and-oxidation in muscles composed primarily of type I fibers, type IIAfibers, and type IIB fibers. In addition, severe LVD induced areduction in the activity of PFK in type IIB muscle, a 10% reductionin the percentage of type IID/X fibers, and a corresponding increase inthe portion of type IIB fibers. Atrophy of type I fibers, type IIAfibers, and/or type IIB fibers occurred in soleus and plantarismuscles of rats with severe LVD. These data indicate that rats withsevere LVD after myocardial infarction exhibit1) decrements in mitochondrialenzyme activities independent of muscle fiber composition,2) a reduction in PFK activity in type IIB muscle, 3) transformationof type IID/X to type IIB fibers, and4) atrophy of type I, IIA, and IIBfibers.

  相似文献   

13.
We examined the effect of the2-agonist clenbuterol (50 µM)on depolarization-induced force responses and sarcoplasmic reticulum (SR) function in muscle fibers of the rat (Rattusnorvegicus; killed by halothane overdose) that had beenmechanically skinned, rendering the2-agonist pathway inoperable.Clenbuterol decreased the peak of depolarization-induced forceresponses in the extensor digitorum longus (EDL) and soleus fibers to77.2 ± 9.0 and 55.6 ± 5.4%, respectively, ofcontrols. The soleus fibers did not recover. Clenbuterol significantlyand reversibly reduced SR Ca2+loading in EDL and soleus fibers to 81.5 ± 2.8 and 78.7 ± 4.0%, respectively, of controls. Clenbuterol also producedan ~25% increase in passive leak ofCa2+ from the SR of the EDL andsoleus fibers. These results indicate that clenbuterol has directeffects on fast- and slow-twitch skeletal muscle, in the absence of the2-agonist pathway. Theincreased Ca2+ leak in the triadregion may lead to excitation-contraction coupling damage in the soleusfibers and could also contribute to the anabolic effect of clenbuterolin vivo.

  相似文献   

14.
Emerson, Geoffrey G., and Steven S. Segal. Alignment ofmicrovascular units along skeletal muscle fibers of hamster retractor.J. Appl. Physiol. 82(1): 42-48, 1997.When muscle fibers contract, blood flow requirements increasealong their entire length. However, the organization of capillaryperfusion along muscle fibers is unclear. The microvascular unit (MVU)is defined as a terminal arteriole and the group of capillaries itsupplies. We investigated whether neighboring MVUs along the fiber axis perfused the same group of muscle fibers by using the parallel-fibered retractor muscle. Hamsters were anesthetized and perfused with Microfilto visualize MVUs relative to muscle fibers. Fields of study, whichencompassed five to seven neighboring MVUs along a muscle fiber, werechosen from the interior of muscles and along muscle edges. On average,MVUs were 1 mm in length, 0.50 mm in width, and 0.1 mm deep; segmentsof ~30 fibers were contained in this tissue volume of 0.05 mm3 (20 MVUs/mg muscle). The totaldistance across muscle fibers encompassed by a pair of MVUs isdesignated "union" (U); the fraction of this distance common toboth MVUs is designated "intersection" (I). The ratio of I to Ufor the widths of neighboring MVUs provides an index of MVU alignmentalong muscle fibers (e.g., I/U = 1.0 indicates complete alignment,where the fibers perfused by one MVU are the same as those perfused bythe neighboring MVU). We found that I/U along muscle edges (0.71 ± 0.02) was greater (P < 0.05) thanthe ratio measured within muscles (0.66 ± 0.02). A model predicteda maximum I/U of 0.58 with random MVU alignment. Thus measured valueswere closer to random than to complete alignment. These findingsindicate that an increase in blood flow along muscle fibers requiresthe perfusion of many MVUs and imply that vasodilation is coordinatedamong the parent arterioles from which corresponding MVUsarise.

  相似文献   

15.
Elevated levels of Pi are thought to cause a substantial proportion of the loss in muscular force and power output during fatigue from intense contractile activity. However, support for this hypothesis is based, in part, on data from skinned single fibers obtained at low temperatures (15°C). The effect of high (30 mM) Pi concentration on the contractile function of chemically skinned single fibers was examined at both low (15°C) and high (30°C) temperatures using fibers isolated from rat soleus (type I fibers) and gastrocnemius (type II fibers) muscles. Elevating Pi from 0 to 30 mM at saturating free Ca2+ levels depressed maximum isometric force (Po) by 54% at 15°C and by 19% at 30°C (P < 0.05; significant interaction) in type I fibers. Similarly, the Po of type II fibers was significantly more sensitive to high levels of Pi at the lower (50% decrease) vs. higher temperature (5% decrease). The maximal shortening velocity of both type I and type II fibers was not significantly affected by elevated Pi at either temperature. However, peak fiber power was depressed by 49% at 15°C but by only 16% at 30°C in type I fibers. Similarly, in type II fibers, peak power was depressed by 40 and 18% at 15 and 30°C, respectively. These data suggest that near physiological temperatures and at saturating levels of intracellular Ca2+, elevated levels of Pi contribute less to fatigue than might be inferred from data obtained at lower temperatures. skinned single fiber; force; power  相似文献   

16.
Allen, David L., Jon K. Linderman, Roland R. Roy, Richard E. Grindeland, Venkat Mukku, and V. Reggie Edgerton. Growth hormone/IGF-I and/or resistive exercise maintains myonuclearnumber in hindlimb unweighted muscles. J. Appl.Physiol. 83(5): 1857-1861, 1997.In the presentstudy of rats, we examined the role, during 2 wk ofhindlimb suspension, of growth hormone/insulin-like growth factor I(GH/IGF-I) administration and/or brief bouts of resistance exercise in ameliorating the loss of myonuclei in fibers of the soleusmuscle that express type I myosin heavy chain. Hindlimb suspensionresulted in a significant decrease in mean soleus wet weight that wasattenuated either by exercise alone or by exercise plus GH/IGF-Itreatment but was not attenuated by hormonal treatment alone. Both meanmyonuclear number and mean fiber cross-sectional area (CSA) of fibersexpressing type I myosin heavy chain decreased after 2 wk of suspensioncompared with control (134 vs. 162 myonuclei/mm and 917 vs. 2,076 µm2, respectively). NeitherGH/IGF-I treatment nor exercise alone affected myonuclear number orfiber CSA, but the combination of exercise and growth-factor treatmentattenuated the decrease in both variables. A significant correlationwas found between mean myonuclear number and mean CSA across allgroups. Thus GH/IGF-I administration and brief bouts of muscle loadinghad an interactive effect in attenuating the loss of myonuclei inducedby chronic unloading.

  相似文献   

17.
The purpose of this study was to examine the effect of prolongedbed rest (BR) on the peak isometric force(Po) and unloaded shorteningvelocity (Vo)of single Ca2+-activated musclefibers. Soleus muscle biopsies were obtained from eight adult malesbefore and after 17 days of 6° head-down BR. Chemicallypermeabilized single fiber segments were mounted between a forcetransducer and position motor, activated with saturating levels ofCa2+, and subjected to slacklength steps. Vowas determined by plotting the time for force redevelopment vs. theslack step distance. Gel electrophoresis revealed that 96% of the pre-and 87% of the post-BR fibers studied expressed only the slow type Imyosin heavy chain isoform. Fibers with diameter >100 µm made uponly 14% of this post-BR type I population compared with 33% of thepre-BR type I population. Consequently, the post-BR type I fibers(n = 147) were, on average, 5%smaller in diameter than the pre-BR type I fibers(n = 218) and produced 13% lessabsolute Po. BR had no overalleffect on Po per fibercross-sectional area(Po/CSA), even though halfof the subjects displayed a decline of 9-12% inPo/CSA after BR. Type Ifiber Voincreased by an average of 34% with BR. Although the ratio of myosinlight chain 3 to myosin light chain 2 also rose with BR, there was nocorrelation between this ratio andVo for either thepre- or post-BR fibers. In separate fibers obtained from the originalbiopsies, quantitative electron microscopy revealed a 20-24%decrease in thin filament density, with no change in thick filamentdensity. These results raise the possibility that alterations in thegeometric relationships between thin and thick filaments may be atleast partially responsible for the elevatedVo of the post-BRtype I fibers.

  相似文献   

18.
We tested the hypothesis thatage-associated decline in muscle function is related to a change inmyosin ATPase activity. Single, glycerinated semimembranosus fibersfrom young (8-12 mo) and aged (32-37 mo) Fischer 344 × Brown Norway male rats were analyzed simultaneously for force andmyosin ATPase activity over a range of Ca2+ concentrations.Maximal force generation was ~20% lower in fibers from aged animals(P = 0.02), but myosin ATPase activity was not different between fibers from young and aged rats: 686 ± 46 (n = 30) and 697 ± 46 µM/s (n = 33) (P = 0.89). The apparent rate constant for thedissociation of strong-binding myosin from actin was calculated to be~30% greater in fibers from aged animals (P = 0.03),indicating that the lower force produced by fibers from aged animals isdue to a greater flux of myosin heads from the strong-binding state tothe weak-binding state during contraction. This is in agreement withour previous electron paramagnetic resonance experiments that showed areduced fraction of myosin heads in the strong-binding state during amaximal isometric contraction in fibers from older rats.

  相似文献   

19.
Chemically skinned muscle fibers,prepared from the rat medial gastrocnemius and soleus, were subjectedto four sequential slack tests in Ca2+-activating solutionscontaining 0, 15, 30, and 0 mM added Pi. Pi (15 and 30 mM) had no effect on the unloaded shortening velocity (Vo) of fibers expressing type IIb myosin heavychain (MHC). For fibers expressing type I MHC, 15 mM Pi didnot alter Vo, whereas 30 mM Pireduced Vo to 81 ± 1% of the original 0 mM Pi value. This effect was readily reversible whenPi was lowered back to 0 mM. These results are notcompatible with current cross-bridge models, developed exclusively fromdata obtained from fast fibers, in which Vo isindependent of Pi. The response of the type I fibers at 30 mM Pi is most likely the result of increased internal drag opposing fiber shortening resulting from fiber type-specific effects ofPi on cross bridges, the thin filament, or therate-limiting step of the cross-bridge cycle.

  相似文献   

20.
Zachaová, Gisela, HelenaKnotková-Urbancová, Pavel Hník, andTomá Soukup. Nociceptive atrophy of the rat soleus muscle induced by bone fracture: a morphometric study.J. Appl. Physiol. 82(2): 552-557, 1997.Reflex atrophy of the soleus muscle induced by ipsilateralmetatarsal bone fracture in Sagatal-anesthetized adult male rats wasstudied by using two-dimensional stereological methods 7 days after theoperation. When compared with contralateral solei, the wet weight ofthe experimental soleus muscles was decreased by ~24% and the areaof the entire muscle section by ~29%. In atrophied solei, the numberof type 1 fibers was lower by ~8%, resulting in lower total numberof fibers (by ~6%). This indicates that slow motor units might bemore sensitive to nociceptive stimulation. However, with respect to thefiber area, the reflex atrophy induced by metatarsal bone fracture inthe rat soleus muscle resembles simple atrophy after 7 days, as themean muscle fiber area was decreased by ~26% with no significantdifference between atrophy in type 1 and type 2a fibers (by 27.3 and23.0%, respectively).

  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号