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Transcriptional specificity of a multisubunit RNA polymerase induced by Bacillus subtilis bacteriophage PBS2. 总被引:3,自引:3,他引:0 下载免费PDF全文
S Clark 《Journal of virology》1978,25(1):224-237
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Imbert I Guillemot JC Bourhis JM Bussetta C Coutard B Egloff MP Ferron F Gorbalenya AE Canard B 《The EMBO journal》2006,25(20):4933-4942
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Sylvie Grandemange Sophie Schaller Shigeru Yamano Stanislas Du Manoir George V Shpakovski Marie-Geneviève Mattei Claude Kedinger Marc Vigneron 《BMC molecular biology》2001,2(1):14
Background
The sequences encoding the yeast RNA polymerase II (RPB) subunits are single copy genes. 相似文献8.
Sylvie Grandemange Sophie Schaller Shigeru Yamano Stanislas Du Manoir George V Shpakovski Marie-Geneviève Mattei Claude Kedinger Marc Vigneron 《BMC molecular biology》2001,2(1):1-11
Background
Some origins in eukaryotic chromosomes fire more frequently than others. In the fission yeast, Schizosaccharomyces pombe, the relative firing frequencies of the three origins clustered 4-8 kbp upstream of the ura4 gene are controlled by a replication enhancer - an element that stimulates nearby origins in a relatively position-and orientation-independent fashion. The important sequence motifs within this enhancer were not previously localized. 相似文献9.
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Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase. 总被引:9,自引:5,他引:4 下载免费PDF全文
The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi 6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small single-stranded RNAs were synthesized from each double-stranded RNA. 相似文献
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Single crystals of bacteriophage T7 RNA polymerase 总被引:1,自引:0,他引:1
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination. 相似文献
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