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1.
- Two neutral plant hormones, one isolated recently from plants(3-indolylacetonitrile) and the other (3-indolylacetaldehyde)reported to be present in plants, are avaible as pure syntheticcompounds for investigation of their biological activities.This paper is mainly concerned with their effects on cellelongationin the Avena coleoptile
- 3-Indolylacetaldehyde is considerablyless active than 3-indolylaceticacid in the Avena straight-growthtest; for example, a 1.0 mg./l.solution of the aldehyde showsan activity equivalent to thatof a 0.1 mg./l. solution of theacid
- An acidic substance is produced in solutions of the aldehydeduring the period of assay. In some experiments it accountsfor all of the activity shown by the aldehyde solutions, onthe assumption that it is 3-indoylacetic acid, and in otherexperiments it shows a greater activity than that of the aldehydesolutions from which it was obtained. Therefore, it is concludedthat 3-indolylacetaldehyde, itself is either inactive or inhibitory.Acid production in aldehyde solutions in vitro is much lower,a fact which suggests that there is enzymatic oxidation of aldehydeto acid in the presence of coleoptiles.
- The activities of3-indolylacetaldehyde in the pea test andin root-inhibitionand of 3-indolylacetone in the straight-growthtest are brieflyreported.
- 3-Indolylacetonitrile is considerably more activethan 3-indolylaceaticacid in the Avena straight-growth test;for erample, a 0.1 mg./l.solution of the nitrile shows an activityequivalent to a 1.0mg./l. solution of the acid. The inhibitoryeffect at concentrationsabove 1.010.0 mg./l. is lesswith the nitrile than withthe acid.
- There is negligible productionof acid in solutions of the nitrileboth in vitro and in thepresence of Avena coleoptiles at temperaturesranging from 18?to 25? C. for varying lengths of time.The possibility of enzymaticconvesion of nitrile to acid insidethe cells of the coleoptileis discussed
- The activities of 3-indolylacetamide and of 2:4-dichlorophenoxyaceticacid and the corresponding nitrile are considered in this connexion
- The nitrile is destroyed by treatment with alkali but notbyacid. In the light of these results, it is desirable to re-examineprevious work on identification of auxins in plants by theiracid and alkali sensitivity. Evidence for the existence of thenitrile in a number of other plants is presented.
2.
- Methods of auxin assay using the Avena coleoptile are discussed.
- A review is given of experimental procedure and evaluationofresults in the straight-growth method using isolated sectionsof coleoptiles in test solutions.
- Possible sources of variationin the straight-growth methodare investigated and discussed.
- A revised experimental procedure for the straight-growth methodis described.
3.
Mode of action of FC was compared with that of auxin in differentexperimental systems and the following results were obtained.
- FC, as well as auxin, primarily induced elongation of the epidermisof pea epicotyl segments, but it also promoted elongation ofthe inner tissue, as judged by its action in split stem tests,elongation of hollow-cylinder segments and elongation of unpeeledand peeled segments.
- FC decreased the minimum stress relaxationtime (T0) and increasedthe extensibility (mm/gr) of the epidermalcell wall of peaepicotyl segments, as did auxin.
- FC failedto induce expansion growth of Jerusalem artichoketuber sliceswhen given alone or in combination with kinetinor gibberellicacid.
- FC at concentrations lower than 106 M, when givenwithauxin at concentrations lower than 0.03 mg/liter, promotedelongationof Avena coleoptile segments in an additive manner,to achievethe maximum elongation at higher concentrations.
- An antiauxin, 2,4,6-trichlorophenoxyacetic acid, inhibitedtheelongation of Avena coleoptile segments due to auxin butnotthat due to FC.
- Nojirimycin, an inhibitor of ß-glycosidases,inhibitedelongation of pea internode segments due not onlyto auxin butalso to FC.
- At concentrations more than 105MFC promoted root elongationof intact lettuce seedlings, whichwas inhibited by exogenousauxin.
4.
Effects of H-ol and H-acid were observed using excised partsof several plant species. Both H-ol and H-acid were active inelongation of oat coleoptile and mesocotyl, expansion of Raphanusleaf disk, but were inactive in elongation of wheat coleoptileand of green stem of pea. They gave also negative results inthe standard Avena curvature test and in the split pea test.In expansion of lettuce cotyledon, H-ol was inactive while H-acidwas active. In excised plant parts, as in intact plants, theactivity of H-ol and H-acid resembles rather gibberellins thanauxins and cytokinins. (Received August 20, 1966; ) 相似文献
5.
- Phyllosinol is a phytotoxic metabolite of Phyllosticta sp. Thissubstance at 100 µg/ml produced dark grey necrotic lesionson the leaf of red clover. Sensitivities of various plant speciesto phyllosinol differed both quantitatively and qualitatively.
- Phyllosinol reduced root growth in rice seedlings by 60% at104 M, whereas stimulation of root elongation occurredat a concentration range from 109 to 105 M.
- Phyllosinolat 2.5x104M promoted adventitious root formationin epicotylsof Azukia cuttings by about 100%. Promotion waspartly reducedby simultaneous application of cysteine.
- IAA-induced elongationof isolated Avena coleoptile sectionswas inhibited by phyllosinolat a concentration range from 105to 103M.
- Sulfhydrylcompounds, i.e. cysteine and glutathione relievedinhibitioncaused by phyllosinol in IAA-induced elongation ofAvena coleoptilesections.
- GA3-induced elongation of wheat leaf sections wasslightly inhibitedby phyllosinol at 104M.
- Phyllosinolalso has antibiotic activity. Among the organismstested, Phycomycetesand Gram-negative bacteria appeared mostsusceptible to phyllosinol.
6.
- MH was found to suppress the growth and respiration of E. colias well as the IAA-induced growth of Avena coleoptile sections.
- These suppressions could be reversed more or less strikinglyby the addition of a trace of heavy metals such as Co, Mn, Ni,Zn, Cu, or Mo.
- The reversal could also be achieved by cysteine,thioglycollate,or fumarate, the latter two substances being,however, lesseffective.
- The inhibition of the growth of E.coli by MH was completelyrelieved by the addition of IAA. Conversely,the inhibitionof the microbial growth by high concentrationsof IAA couldbe relieved by the addition of MH.
- It was inferredthat MH may block certain heavy metal-catalyzedprocess, inwhich some thiol substance and IAA are participating,probablyby combining with the heavy metal.
7.
Previous views on the physical basis of phototropism in Phycomycessporangiophores are briefly discussed.
- It was confirmed thatunilaterally illuminated sporangiophoresimmersed in liquidparaffin show strong negative phototropism.
- Elongation growthceased and no phototropic response took placeunder anaerobicconditions.
- By focusing a fine beam of light on to one edgeof the growingzone of a sporangiophore, leaving the other sidein darkness,it was established that greater elongation tookplace in theilluminated zone, the sporangiophore tending tobend out ofthe beam. Rapid reversal of the curvature followedwhen theillumination was transferred to the opposite edge ofthe sporangiophore.
8.
- Apprehension over the adequncy of current techniques stimulateda detailed study of the time factor in the arsenate inhibitionof growth and respiration in excised stem and root sectionsof Pisum sativum.
- Growth inhibition by arsenate sets in veryslowly, its rateof onset being related to the molar concentration(C) of arsenateate by the relation where T50 is the time taken in hours to reduce the growthrateto 50 per cent of the control and K is a constant. An explanationof the physiological basis of this relationship is attempted.
- Estimates were made of the final steady growth rate (relativeto control) in various arsenate concentrations. The inhibitionscalculated from this rate are held to approximate to the truearsenate effect and are shown to be very different from thosecalculated from total growth measures.
- Respirationof growing stem sections is not inhibited by thelow arsenateconcentrations that inhibit growth. Some inhibitionis indicatedat high concentrations (3 ? 104M. and over)but onlyafter 15-20 hours of exposure.
- Two per cent sucrose has noeffect on the arsenate inhibiitionof stem growth. Sucrose,however, markedly stimulates respirationin stem sections, butthis stimulation is prevented by arsenate.
- The misinterpretationswhich may arise as a result of ignoringthe time factor in inhibitionstudies in excised organ sectionsare discussed and the desirabilityof constructing completegrowth curves in all such studies isstressed.
9.
SHIBAOKA HIROH; ANZAI TADASHI; MITSUHASHI MIEKO; SHIMOKORIYAMA MASAMI 《Plant & cell physiology》1967,8(4):647-656
- Heliangine at 1104 M promoted the adventitious rootformation in hypocotyls of cuttings taken from light-grown (1,900lux) Phaseolus mungo seedlings. The promotion was almost completelyreversed by 3104 M uracil, uridine, cytidine, oroticacid or 6104 M carbamoyl DL-aspartic acid, and partlyby 3104 M thymine or thymidine. Neither 3104M cytosine, adenine, adenosine, guanine, guanosine nor a combinationof 3104 M carbamoyl phosphate and 3104 M L-asparticacid reduced the promotion by heliangine.
- Uracil did not reducethe inhibiting effect of heliangine onthe indoleacetic acidinduced elongation of etiolated Avenacoleoptile sections.
- Helianginein an aqueous uracil solution was recovered unchangedafter24-hr incubation at room temperature.
- The root formation ofPhaseolus cuttings was promoted also by2-thiouracil and 5-fluorouracil.The effect was reversed byorotic acid or carbamoyl asparticacid, but not by carbamoylphosphate plus aspartic acid.
- Ribonucleaseat 100 µg/ml increased the number of rootsprotruded fromhypocotyls of cuttings by about 260%.
- A possible interpretationfor the promotion of root formationby heliangine is offered.
10.
Studies on Plant Growth Hormones: III. APPLICATION OF ENZYME REACTION KINETICS TO CELL ELONGATION IN THE AVENA COLEOPTILE 总被引:1,自引:0,他引:1
- Previous reports that Michaelis enzyme kinetics may be appliedto the system controlling cell elongation in Avena coleoptilesare confirmed.
- Substrate (applied hormone) and enzyme arenot in direct contactwith each other. Active transport of theapplied hormone incoleoptile sections, and permeability ofcells to hormone, regulatethe intracellular substrate concentration,thereby markedlyinfluencing the value of K5, a parameter givinga measure ofthe affinity of substrate for enzyme.
- The effectof permeability and transport factors on calculatingK1 valuesof competitive hormone inhibitors is considered. Dataof otherworkers are used to show that observed K1 values arenot necessarilyindependent of the hormone used.
- Auxin-induced inhibitionof cell elongation results primarilyfrom a toxic action ofhormones on cell protoplasm leading finallyto cellular disorganization,shrinkage, and death. The datado not decisively support thehypothesis of inhibition resultingfrom steric hindrance totwo-point attachment between substrateand enzyme.
11.
- 1. The growth rate of Avena coleoptile sections in the presenceof indoleacetic acid (IAA) is constant with time over a widerange of time intervals and IAA concentrations.
- 2. Constancyof growth rate is dependent upon the maintenanceof constantconditions in which the concentration of IAA availableto thesection remains the chief factor limiting growth rate.
- 3.Control of the pH of the medium in which the sections aregrownis essential to the maintenance of constant growth rate,particularlyin the presence of high concentrations of IAA.
- 4. The lagperiod in establishment of steady growth rate bysections inthe presence of IAA is less than 10 minutes andis not detectableby present methods of measurement.
12.
OSBORNE DAPHNE J.; BLACKMAN G. E.; NOVOA S.; SUDZUKI F.; POWELL R. G. 《Journal of experimental botany》1955,6(3):392-408
Anumber of 2 : 6-substituted phenoxyacetic acids in which theside chain had been substituted by ailcyl groups to form -propionic,-n-butyric and -n-valeric acids were investigated to assesstheir ability to act as growth-regulators. Besides employingstandard tedmiques of bioassay, further experiments were undertaken to determine the effects of these compounds on the vegetativegrowth and development of intact plants of Helianthus annuus. It has been established that all the compounds tested inducedcurvature in the Went pea curvature test and that without exceptionthe activity of the parent phenoxyacetic acid (2:6-dichloro-,2:4:6-trichloro-,2:6-dimethyl-, and 2:4-dichloro-6-methyl-)was increased by side chain substitution. In the Avena straight growth assay, the a 2:6-dichloro- and2:6-dimethyl- phenoxypropionic and butyric acids brought aboutstatistically significant increases in length of the coleoptilesections, when measurements were made at the end of 24 hours.Their activity was of the same order as that exhibited by a2:4-dichlorophenoxyacetic acid. Investigations were carried out on the uptake of water by sectionsof pea internode and the extension growth of Avena coleoptileaectiona when both the concentration and the length of treatmentwere varied. For some compounds it was found that over a certainrange of concentration water uptake and extension growth wereaccelerated in the initial 68 hours. Subsequent to this,inhibition occurred so that no significant increases above controlvalues were apparent at the end of 24 hours, and in some casesan actual loss of water or shrinkage in length took place. Itwas thus possible to demonstrate that 2:6-dimethylphenoxyaceticacid acts as a growth regulator in pea extension growth andthat 2:6-dichiorophenoxyacetic acid is active in the Avena test. The changes in the leaf area of individual pairs of leaves,together with the lengths of the internodes and shoot weightwere followed after application of measured amounts of eachcompound to the first pair of leaves of H. annuus. A numberof the 2:6-compounds were capable of modifying growth in wayssimilar to 2:4-dichlorophenoxyacetlc acid. However, much higherconcentration had to be applied in order to produce effectscomparable to those caused by the 2:4-dichloro- compound. Measurementsof the percentage of applied compound which penetrated intothe leaf showed that there were no marked differences in thisrespect between the different phenoxy acids. On the other hand,experiments in which the treated leaves were left on the plantfor varying periods of time led to the conclusion that the a: 6-substituted compounds are less readily translocated than2:4-dichlorophenoxyacetic acid. 相似文献
13.
- A method is described by means of which several grammes ofl-quinicacid were isolated from young fruits of the WorcesterPearmainapple stored for a number of days in nitrogen.
- Theessential stages in the method are the removal of colouringmatter from extracts of the frozen ground pulp tissue of thefruit with charcoal, the removal of amino acids by absorptionon cation exchange resin, and the separation of the organicacid from sugars by adsorption of the former on anion exchangeresin. Finally, the quinic acid was separated from malic acidby fractional displacement from the anion exchange resin.
- Thecharacterization of the isolated quinic acid, and of malicacidalso isolated from the fruits, is described.
- Citric acid isshown to be formed by oxidation of quinic acidwith hot hydrogenperoxide.
- The possible function of quinic acid in the applefruit andin plants generally is discussed.
14.
- The organic acids present are citric, isocitric, and l-malic,with a small residue of unidentified acids.
- The diurnal variationin acidity is due chiefly to changes,in malic acid, with aparallel fluctuation shown by citric acid.Under these conditionsisocitric acid shows little change.
- The importance of carbondioxide during acidification is confirmed,and it is shown thatat room temperatures or higher the CO2produced in respirationis sufficient to produce maximum acidification.At lower temperaturesthe supply of CO2 limits acid production.
- In the absence ofoxygen no acidification occurs, but even smallquantities (approx.1 per cent.) are sufficient to cause someacid production.
- Completebalance-sheets are presented for acids, carbohydrates,CO2 andoxygen for leaves maintained in the dark at high andlow temperatures.As acids are produced there is a correspondingloss of carbohydrate(chiefly starch). A scheme of reactionsis suggested to explainthe experimental results.
15.
The auxins contained in 5-mm. tips of horizontal Vicia fabaroots have been compared with those in tips of vertical rootsafter cold ethanol extraction, paper-chromatographic separation,and Avena mesocotyl bioassay. At about the time curvature commencesin horizontal roots there is a marked increase in the contentof an auxin corresponding to AP(ii) of pea roots(Rf 0.350.65 in isobutanol/methanol/water). There areindications that this is not due to its release from an inactivebound state but that it is either synthesized de novo or maybe converted from another auxin corresponding to AP(iii)of pea roots (Rf 0.751.0). The literature dealing with the auxins of geotropically stimulatedorgans is reassessed and it is concluded that, with the exceptionof the Avena coleoptile, there is very little evidence favouringa simple transport redistribution of auxin under gravity; themajority of the data favour an effect of gravity on auxin metabolism. 相似文献
16.
- The metabolism of a citric-acid-forming strain of A. nigerwhengrown on a glucose-acetate medium has been investigated.
- Acetate greatly accelerated the rate of utilization of glucose.
- Citric acid production from glucose was much increased bythepresence of acetate.
- The formation of oxalate from glucose-acetatecultures was muchless than from acetate alone.
- In some cultureslarge amounts of glucose and acetate were consumedbut no acidicproducts were formed.
17.
- The capacity of green leaves for absorbing and converting substancesrelated to L-ascorbic acid was investigated, using detachedleaves of various plants, including soybean, pea, barley andspinach.
- The greater portion of the absorbed dehydroascorbicacid wasrecovered in the leaves as ascorbic acid, minor portionsbeingdiscovered as dehydroascorbic acid and 2,3-diketogulonicacid. The recovery was about 60% of the absorbed dehydro ascorbicacid, in the cases of detached soybean and barley leaves, forinstance, thus suggesting the instability of the substance invivo.
- The absorption and conversion of ascorbic acid and 2,3-di-ketogulonic acid in detached green leaves were also investigated.Most of the absorbed ascorbic acid reappeared in the leaves.On the other hand, no evidence for the conversion of 2,3-diketogulonicacid into ascorbic and dehydroascorbic acids was observed. Thegreater portion of the absorbed 2, 3-diketogulonic acid seemsto be decomposed in the leaves, under the condition of the presentstudy.
18.
- A substance which inhibits indoleacetic acid (IAA)-and naphthaleneaceticacid (NAA)-induced elongation of Avena coleoptile section andIAA-induced Avena coleoptile curvature was found in an ethersoluble neutral fraction of water extract of sunflower leavesand in agar blocks containing the diffusate from young sunflowerleaves.
- This substance also inhibits the growth of isolatedsunflowerepicotyl.
- The Rf value (0.9) of the substance ona paper chromatogramdeveloped with ammoniacal iso-propanolindicates that it isidentical with the inhibitor reported byAUDUS et al. (1956),but not with inhibitor-ß.
- Theinhibitor can be transported from leaf to stem, and thetransportseems to be accelerated by illuminating the leaf.
- The auxindiffused from sunflower leaf into agar block may beidenticalwith IAA.
- A substance, which has the same properties as theinhibitorfrom sunflower leaf, was obtained in crystalline formfrom theleaf of Jerusalem artichoke.
- The mechanism of growthinhibition caused by this crystallinesubstance seems to involveinactivation of a sulfhydryl group.
- The reason why the stemgrowth of sunflower seedlings is reducedby strong light isdiscussed: the amount of the inhibitor transportedfrom leafto stem is increased under strong light, and in thestem, growthinhibition is caused by a direct effect of thisinhibitor ongrowth and by its inhibiting effect on the transportof IAAfrom leaf to stem.
19.
- The woody shoots of young saplings of Fraxinus.excelsior andAcer Pseudo-platanus in pots were subjected to continuous coolingto about 2° C. during the growth season, with the resultthat radial growth was almost completely inhibited throughoutthe woody stem.
- The chilling did not adversely affect extensiongrowth exceptthat it was later in commencement and proceededmore slowly.
- If the temperature around the stem is loweredfrom 2° C.to 0° C., water conduction is cut down tosuch an extentas to cause wilting of the leafy shoots; turgidityis recoveredwhen the temperature is again raised to 2°C.
- This wilting effect is discussed particularly in relationtothe part played by living cells in the upward movement ofwaterin the wood.
20.
Effect of Peeling on IAA-induced Growth in Avena Coleoptiles 总被引:1,自引:0,他引:1
The act of peeling removes the epidermis exclusively from Avenacoleoptiles. Peeling inhibits IAA-induced growth, by inhibitingthe growth of segments incubated in the presence of IAA, andpromoting that of those incubated in water. The magnitude ofthe inhibition of IAA-induced growth is proportional to theamount of epidermis removed. It is shown that neither lateralswelling, wounding, anaerobiosis, nor exposure to supraoptimalconcentrations of IAA cause the inhibition. It is concludedthat in Avena coleoptiles the epidermis regulates the rate ofexpansion of the underlying parenchyma cells and is the principaltarget of IAA-action. Avena sativa L., oat, coleoptile, indol-3-ylacetic acid, auxin, extension growth 相似文献