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1.
Summary A method is described whereby the differential staining of viable and nonviable unfixed cells, as observed by the dye-exclusion
method, can be reproduced in glutaraldehyde-fixed preparations by staining with alcian blue. The results suggest that the
differential staining is due, at least in part, to structural differneces that are retained following aldehyde fixation.
This work was supported by grants from the National Cancer Institute of Canada and the National Research Council of Canada. 相似文献
2.
Patricia A. Kruk Nelly Auersperg 《In vitro cellular & developmental biology. Animal》1991,27(4):273-276
This work was supported by a grant and research associateship to N.A. from the Medical Research Council of Canada and the
National Cancer Institute of Canada. 相似文献
3.
Graeme J. Dougherty J. Dean Thacker Robert S. Lavey Arie Belldegrun William H. McBride 《Cancer immunology, immunotherapy : CII》1994,38(5):339-345
In order to define the potential antitumor activity of the multifunctional cytokine interleukin-6 (IL-6), retrovirus-mediated gene transfer was used to introduce and express a cDNA encoding human IL-6 in the murine fibrosarcoma cell line Fsa-R. Although these genetically modified tumor cells appeared morphologically and phenotypically identical to control Fsa-R cells and had a similar plating efficiency in vitro, they were found to exhibit greatly reduced tumorigenicity in vivo following intravenous injection into syngeneic recipients. Exogenous IL-6 was shown to produce a similar inhibition of tumor growth in the lung if administered intraperitoneally. In contrast, tumor growth in subcutaneous sites was inhibited only if the tumor cells were engineered to express IL-6 locally, or if IL-6 was administered intratumorally. Intraperitoneal injection of IL-6 had no inhibitory effect. Tumors that did grow from IL-6-producing tumor cell inocula in subcutaneous sites were found to contain large numbers of macrophages. These results demonstrate that the antitumor activity of systemically administered IL-6 varies depending on the site of tumor growth and suggest an important role for IL-6 in the recruitment, proliferation and/or survival of tumor-associated macrophages.Supported by grants from the B. C. Health Care Research Foundation (BCHRF), the National Cancer Institute (NCI) and the National Cancer Institute of Canada (NCIC). G.J.D. is a Research Scientist of the National Cancer Institute of Canada (NCIC). R.S.L. is a RSNA Research and Education Fund Scholar 相似文献
4.
Summary During methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells, proliferation is inhibited, morphologic
and biochemical changes occur, and giant, often multinucleated, cells form. We have used the increase in cell volume as a
marker of the mature syncytiotrophoblastlike phenotype. Uninduced and differentiated BeWo cells are not spherical, and theoretical
considerations suggested that deviations in shape could result in significant errors in Coulter volume. To determine if the
values obtained by electrical pulse sizing reflected the actual mass of BeWo cells, we have evaluated the relationship between
Coulter volumes and intracellular water volumes obtained using a shape-independent estimate for eight cell types. A close
correlation (r
2=0.97) was found, indicating that cell volume changes in populations of irregularly shaped cells can be accurately measured
using a Coulter instrument.
Supported by an operating grant from the National Cancer Institute of Canada. N.S.B. was a recipient of a studentship award
from the Alberta Heritage Foundation for Medical Research. C.E.C. is a Senior Research Scientist of the National Cancer Institute
of Canada. The McEachern Laboratory is a research facility of the Faculty of Medicine, University of Alberta, and the Cross
Cancer Institute, Edmonton, Alberta. 相似文献
5.
Two major classes of alcohol dehydrogenase isozymes have been purified from Chinese hamster liver. The two isozyme classes have the same subunit molecular weights but different electrophoretic mobilities. They have a similar range of substrates but different KROM values and sensitivities to the inhibitor pyrazole. The ADHs are immunologically related as determined by Ouchterlony double diffusion experiments. These results suggest that the isozymes are encoded by different structural gene loci derived from a common ancestral gene.Financial support was provided by the National Cancer Institute of Canada and the Medical Research Council of Canada, Grant MT4860. J.-P. Thirion is a Research Scholar of the Science Research Council of Quebec. 相似文献
6.
Jill M. Siegfried Karen G. Nelson Jane L. Martin David G. Kaufman 《In vitro cellular & developmental biology. Plant》1984,20(1):25-32
Summary Histochemical techniques have been applied to the identification of cell types cultured from human endometrium. Previous work
from this laboratory characterized two principtal cell types found in cultures of endometrium: a mature epithelial cell and
another cell which was classified as the endometrial stromal cell based on light and electron microscopy. In this report we
compare the histochemical staining of endometrial tissue in frozen sections to that of cultured cells. These results confirm
the epithelial and stromal nature of the respective cell types. Several markers were found that could distinguish between
cells of epithelial and stromal origin. The enzymes alkaline phosphatase, γ-glutamyltranspeptidase, peroxidase, and β-glucuronidase
were localized in glandular and surface epithelia in frozen sections and in colonies of epithelial cells in culture. Stroma
in frozen sections and cultured stromal cells contained leucine aminopeptidase and fibronectin. Epithelial sections and in
culture could also be distinguished from cells of stromal origin by preferential binding of lotus and peanut lectin. Several
other markers were found in both endometrial epithelium and stroma.
J. M. S. was recipient of National Research Service Award CA09156 (National Cancer Institute); K. G. N. was recipient of National
Research Service Award ES07017 (National Institute of Environmental Health Sciences); and D. G. K. was recipient of Research
Career Development Award CA00431 from the National Cancer Institute, Bethesda, MD. Supported by Grant CA 31733 from the National
Cancer Institute, Bethesda, MD. 相似文献
7.
Summary The rudimentary locus of Drosophila melanogaster is shown to be at least bifunctional. Mutants in different regions of the locus have either no CPSase or no ATCase activity; some mutants lack both activities. The results are discussed in correlation with the complementation and genetic map of the locus.Supported by National Research Council of Canada, grant A-1764 and National Cancer Institute of Canada, grant 6051 to D. T. Suzuki.Researcher, Centre National de la Recherche Scientifique, France and recipient of European Molecular Biology Organization Fellowship. 相似文献
8.
R. L. Ceriani J. Taylor-Papadimitriou J. A. Peterson P. Brown 《In vitro cellular & developmental biology. Plant》1979,15(5):356-362
Summary Two major types of cells can be cultured from early lactation human milks: a colony-forming epithelial cell and an adherent
nondividing cell referred to as a foam cell The epithelial cells show a positive reaction with a specific antiserum reactive
against membrane components of the milk fat globule, whereas the foam cells do not. The nondividing foam cells are phagocytic
and can be killed by silica particles; they produce lysozyme, are resistant to trypsinization, and have Fc receptors. These
properties, together with the lack of reaction with antiserum to the milk fat globule membrane, suggest that the foam cells
are not terminally differential epithelial cells, but tissue macrophages.
R. L. C. was supported by Grant No. Ca 19455 from the National Cancer Institute, a Yamagiawa-Yoshida Memorial International
Cancer Study Grant, and the Imperial Cancer Research Fund. J. A. P. was supported by Grant No. CA 19455 from the National
Cancer Institute. 相似文献
9.
Role of different epithelial cell types in liver ontogenesis,regeneration and neoplasia 总被引:6,自引:0,他引:6
Normand Marceau Marie-José Blouin Lucie Germain Micheline Noel 《In vitro cellular & developmental biology. Plant》1989,25(4):336-341
This work is supported by the National Cancer Institute of Canada. 相似文献
10.
Lesley D. Dunfield Trevor G. Shepherd Mark W. Nachtigal 《Biological procedures online》2002,4(1):55-61
Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer
(OC) cell lines exist, however, most have acquired significant genetic alterations from their cells of origin, including deletion
of important cell cycle regulatory genes. In order to analyze signaling events related to cell cycle control in human OC,
we have modified existing protocols for isolating and culturing OC cells from patient ascites fluid and normal ovarian surface
epithelial (OSE) cells from benign ovarian tissue sections. These cells maintain an epithelial phenotype and can be manipulated
experimentally for several passages before cellular senescence. An example using TGF1 treatment of OC cells to examine signaling
and target gene activation is presented.
This work is supported in part by the National Cancer Institute of Canada with funds from the Canadian Cancer Society (#13631),
the Nova Scotia Health Research Foundation, and by Cancer Research and Education (CaRE) Nova Scotia with funding from the
Faculty of Medicine, Dalhousie University. L.D.D. is supported by the Rossetti Studentship for Cancer Research with funds
from the Dalhousie Medical Research Foundation, T.G.S. is supported by a CaRE Fellowship with funding from the Canadian Cancer
Society, and M.W.N is a Canadian Institutes of Health Research Scholar.
Published: October 28, 2002. 相似文献
11.
Philip Skehan James E. Thomas Susan J. Friedman 《In vitro cellular & developmental biology. Plant》1986,22(11):632-636
Summary Sarcoma 180 monolayers spontaneously shed single cells and small multicellular aggregates into the surrounding medium to produce
a dual population of floating and substratum-attached cells. Shedding was a motility-associated event that occurred when cells
attempted to migrate over one another. It resulted from a combination of cell shape change and active motility, which increased
sensitivity to fluid shear dislodgement by reducing a cell's surface area of adhesive contact and increasing strain tension
at its adhesive contact points. Shedding occurred at all phases of the cell cycle. Extracellular matrix but not conditioned
medium enhanced the floating subpopulation by slowing the kinetics of rattachment to plastic and cellular substrata. Although
sarcoma 180 cells are anchorage independent in the sense that they grow readily in single cell suspension, they nevertheless
exhibited anchorage modulation of their cell cycle. Short periods in suspension produced a mild G1 accumulation, whereas longer periods of anchorage deprivation led to a mild G2 accumulation which appeared to result from an interference with cytokinesis.
This work was supported by grants from the Medical Research Council of Canada, The National Cancer Institute of Canada, the
Alberta Heritage Savings and Trust Fund for Applied Cancer Research, and the Alberta Heritage Fund for Medical Research. 相似文献
12.
Temperature-sensitive mutations in Drosophila melanogaster. XIV. A selection of immobile adults 总被引:18,自引:0,他引:18
Thomas A. Grigliatti Linda Hall Raja Rosenbluth David T. Suzuki 《Molecular & general genetics : MGG》1973,120(2):107-114
Summary Improved methods for rearing and screening large numbers of flies permitted the recovery of 10 mutations exhibiting a reversible temperature-dependent adult paralysis among 1.1×106 flies tested. Of the 10 mutations, two were allelic to para
ts,two were alleles in a new locus, stoned (stn), and six fell into a third area, the shibire (shi) locus. Several of the shi alleles cause embryonic, larval and adult paralysis at 29° C as well as structural anomalies of various tissues. In addition to the ts mutations, several non-conditional mutations affecting adult movement were recovered.This work was supported by the National Research Council of Canada, grant A-1764, National Cancer Institute of Canada, grant 6051.Medical Research Council of Canada Postdoctoral Fellow. 相似文献
13.
Wayne F. Flintoff Susan M. Spindler Louis Siminovitch 《In vitro cellular & developmental biology. Plant》1976,12(11):749-757
Summary In a previous report, we described the selection and partial characterization of three methotrexate (Mtx)-resistant Chinese
hamster ovary cells (CHO) (1). Class I cells contained an apparent structural alteration in dihydrofolate reductase. Class
II cells had an alteration affecting the permeability of the drug. Class III cells, selected from Class I cells, had an increased
activity of the altered enzyme. In the work described here, it has been shown that the spontaneous mutation rate to Class
I resistance is in the order of 2 × 10−9 mutations per locus per generation and that in single-step mutagenized selections the number of resistant colonies of Classes
I and II are about equal. Class I and Class III resistance is expressed codominantly in somatic cell hybrids, whereas the
Class II resistant marker is a recessive trait.
Presented in the formal symposium on Somatic Cell Genetics at the 27th Annual Meeting of the Tissue Culture Association, Philadelphia,
Pennsylvania, June 7–10, 1976.
This research was supported by the Medical Research Council of Canada, the National Cancer Institute of Canada and the National
Institutes of Health of the United States. W. F. was a Postdoctoral Fellow of the Medical Research Council of Canada. 相似文献
14.
Joseph A. Davidenas Marvin J. Fritzler Russell E. Giblak Robert B. Church 《In vitro cellular & developmental biology. Plant》1974,9(4):223-229
Summary A new tissue culture cell line (Calg-ARLC) has been established from an explant culture of adult rabbit lung. The Calg-ARLC
line has been characterized with respect to morphology, chromosome constitution, tissue culture requirements, proliferative
capacity, cell cycle, attainable synchrony, radioisotopically labeled precursor incorporation into nucleic acids and protein,
and radioisotope sensitivity. The cells are fibroblast-like in appearance with a stabilized heteroploid chromosomal modal
number of 35. They grow exponentially from high split ratios in several commercially available defined media with a generation
time of 12 hr and are easily synchronized. Although sensitive to some isotopically labeled precursors, high specific activity
nucleic acids have been isolated. The ARLC line is especially useful for the isolation of high specific activity nucleic acids
and proteins of rabbit origin. The Calg-ARLC line should be invaluable in the fractionation of reiterated DNA sequences since
no very rapidly reassociating DNA sequences such as those found in mouse are evident.
This work was supported by operating grants from the National Cancer Institute of Canada and the National Research Council
of Canada. 相似文献
15.
Leon J. Goodman 《Radiation and environmental biophysics》1979,16(4):367-371
Summary When detailed neutron energy spectrum data are lacking for a mixed field of neutrons and photons, it is permissible when estimating the dose equivalent to assume that the quality factor for the neutrons is 10. With this assumption, it is shown that the responses of a tissue-equivalent ionization chamber and a Geiger-Müller dosimeter can be used to obtain an acceptable approximation of the dose equivalent in the mixed field without requiring precise knowledge of the relative neutron sensitivity of the Geiger-Müller dosimeter.This investigation was supported by Contract EP-78-S-02-4733 from the Department of Energy to the Radiological Research Laboratory/Department of Radiology and by Grant No. CA13696 to the Cancer Center/Institute of Cancer Research, awarded by National Cancer Institute, DHEW 相似文献
16.
Harald H. Rossi 《Radiation and environmental biophysics》1981,20(1):1-9
Summary Although extension of the time period during which a given dose of radiation is administered commonly reduces effectiveness, there are well established instances where the reverse is true. Theoretical considerations are presented which relate reduction or enhancement to the shape of the dose-effect curve. While in many instances these changes of sensitivity may be due to intracellular processes it appears that in the case of carcinogenesis by low doses of neutrons, time dependent intercellular action must be involved.This investigation was supported by Contract DE-AC02-78EV04733 from the Department of Energy and by Grant Nos. CA 12536 and CA 15307 to the Radiological Research Laboratory/Department of Radiology, and by Grant No. CA 13696 to the Cancer Center/Institute of Cancer Research, awarded by the National Cancer Institute, DHHS 相似文献
17.
Improved conditions for murine epidermal cell culture 总被引:1,自引:0,他引:1
Susan M. Fischer Aurora Viaje Kris L. Harris Don R. Miller Jeffrey S. Bohrman Thomas J. Slaga 《In vitro cellular & developmental biology. Plant》1980,16(2):180-188
Summary An improved method for cultivating newborn mouse epidermal cells has been developed that increases the longevity, epithelial
nature and efficiency of cell-line establishment. The use of Super Medium, an enriched Waymouth's formulation, increased proliferation
for long periods of time, as did incubation at 31°C rather than 37°C. The fetal bovine serum requirement was found to be reduced
at the lower temperature. An increase in labeling indices was seen when epidermal growth factor (EGF) or the cyclic nucleotides
were added and the presence of EGF receptors was determined. Of the prostaglandins (PG) examined, PGE1 and PGE2 produced the greatest increase in DNA synthesis. The PG precursors, arachidonic and 8,11,14-eicosatrienoic acid, were also
greatly stimulatory. The use of a lethally irradiated 3T3 feeder layer at 31°C proved superior in maintenance of an epithelial
morphology. Subculturable cell lines were established much more readily and reproducibly in carcinogen-treated cultures grown
under the improved conditions.
Research sponsored jointly by the National Cancer Institute under Interagency Agreement YO1-CP-70227 and the Office of Health
and Environmental Research, U.S. Department of Energy, under contract W-7405-eng-26 with the Union Carbide Corporation.
Predoctoral Investigator supported by Grant CA 09104 from the National Cancer Institute.
Postdoctoral Investigator supported by Carcinogenesis Training Grant CA 05296 from the National Cancer Institute. 相似文献
18.
Robert B. Goldberg 《Cell and tissue research》1982,223(3):675-684
Summary Cell separation was used to follow the fate of the cortical cells of the adrenal gland in primary culture, and to assess some of the changes that occur as cells adapt to culture conditions. Primary cultures of rat adrenal gland were dissociated with trypsin and separated by velocity sedimentation at unit gravity. After two days in culture, cells showed a reproducible sedimentation profile consisting of two classes of cells with mean sedimentation rates of 5.8 and 2.1 mm/h, and a third sedimentation peak consisting mainly of nuclei at 0.5mm/h. All populations continued to incorporate 3H-thymidine in relatively constant proportion throughout the culture period, but the relative number of cells in the 2.1 mm/h peak increased two-fold in the last few days of primary culture. Cells labelled in primary culture, but separated after an additional 5 days in secondary culture had lost proportionately more labelled cells from the 5.8 mm peak. The results suggest that cells of the 2.1 mm peak survive longer in culture in a post-replicative condition.Work reported in this paper was performed while the author was a Research Fellow of the National Cancer Institute of Canada in the laboratory of Dr. N. Auersperg, Cancer Research Centre, University of British Columbia, Vancouver, B.C., Canada 相似文献
19.
BackgroundTesticular cancer is the most common malignancy among young men aged 15–44 in Canada. The goal of this analysis was to examine age-period-cohort effects of testicular cancer incidence between 1971 and 2015.MethodsData were collected from the National Cancer Incidence Reporting System and the Canadian Cancer Registry. Birth cohort models were fit using the National Cancer Institute’s web tool. Incidence annual percent changes were estimated using NCI’s Joinpoint Regression Program.ResultsIncidence of testicular cancer in Canada has increased steadily since 1971. A birth cohort effect was observed for men born in the years after 1945. The rate of testicular cancer peaks at age 35 and drops off with increasing age.ConclusionIncidence of testicular cancer has risen dramatically in Canada in recent decades and the cohort effect indicates the need to investigate exposures that have increased since 1945 and that may affect development in young men. 相似文献