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1.
To quantify the transmission of tomato spotted wilt virus (TSWV) by Frankliniella occidentalis, the median acquisition access period (AAP50) and median inoculation access period (IAP50) were determined. These parameters were established using transmission rates obtained after AAPs and in IAPs which both ranged from 5 to 2560 min. An AAP50 of 106 min was found when larvae acquired virus from TSWV-infected Impatiens plants. IAP50s of 58 or 137 min, respectively, were calculated when petunia or Datura stramonium leaf disks were used to test the inoculation efficiency of viruliferous thrips. The virus could successfully be acquired or inoculated in periods of 5 min. Transmission reached an optimum after an AAP of 21.3 h (AAPopt) and in an IAP of 42.7 h (IAPopt). These results show that TSWV can be acquired and transmitted efficiently by F. occidentalis in short feeding periods.  相似文献   

2.
    
Chrysanthemum yellows (CY) phytoplasma is a plant-pathogenic mollicutes belonging to the 16Sr-IB genetic group which infects a variety of dicotyledonous plants and is transmitted in nature by some species of Cicadellidae Deltocephalinae. The transmission characteristics of CY and the factors influencing the vector efficiencies of the leafhoppers Macrosteles quadripunctulatus Kirschbaum and Euscelidius variegatus Kirschbaum are described in the present study using transmission experiments and phytoplasma-specific polymerase chain reaction (PCR) assays. Vector insects were allowed to acquire CY under different experimental conditions and then transferred to healthy test plants for inoculation and/or sampled for DNA extraction and amplification. The transmission efficiency of CY was very high and almost all the leafhoppers became infective following acquisition on CY-infected daisies. The latent period in the vector ranged from 16 to 20 days after the start of the acquisition and infectivity lasted, in general, for life. The PCR assay was successful in detecting CY phytoplasmas in the insects well before they became infective (5 versus 16–18 days) and was used to estimate the proportion of infective insects. When analysed for CY presence by PCR, all the leafhoppers fed for 7–18 days on source daisy reacted positively while, following one day of acquisition, some insects failed to provide amplification. Host-plant species influenced CY acquisition, and daisy appeared a more efficient source for both leafhoppers compared to periwinkle. Life stage did not appear to be critical for CY acquisition, although newly-hatched nymphs of E. variegatus acquired CY less efficiently than fifth instar nymphs.  相似文献   

3.
    
Melanetettix gen. nov. is described from Melanesia for 22 new species of leafhoppers. The new species fall into four groups. New species described are M. bicolor, M. bifidus, M. capitatus, M. curvatus, M. delmoi, M. elongatus, M. maai, M. marginatus, M. ocellatus, M. pallidus, M. rhamphodes, M. roseus, M. semeraroae, M. sinuatus, M. aculeus, M. bifurcatus, M. truncatus, M. clavatus, M. ensiferus, M. nielsoni, M. rotundatus and M. alatus. An identification key to the new species is provided along with discussion of the affinities of Melanetettix, which is close to Scaphoideus Uhler.  相似文献   

4.
    
The leafhopper Matsumuratettix hiroglyphicus (Matsumura) (Hemiptera: Cicadellidae) is the most important vector of sugarcane white leaf (SCWL) phytoplasma that significantly affects the sugarcane crop in Asia. Here, we aimed to study the characteristics of SCWL phytoplasma transmission by M. hiroglyphicus. To this end, the stylet penetration activities performed during the acquisition access period (AAP) and inoculation access period (IAP) were investigated by the direct current electrical penetration graph technique and confirmed by quantitative polymerase chain reaction (qPCR). Additionally, the latent period (LP) of SCWL phytoplasma in the vector was determined by qPCR and localised by fluorescent in situ hybridisation. The results indicated that the acquisition of SCWL phytoplasma occurred during phloem ingestion (waveform D), whereas its inoculation was associated with salivation into the phloem sieve element (waveform C). The minimum AAP was 15 min and the minimum duration of phloem ingestion was 2.35 min. The minimum LP of SCWL phytoplasma in the vector was at least 14 days; then, SCWL phytoplasma moved to the salivary glands of the insect, enabling the transmission of the pathogen to the host plants. The minimum IAP for a successful transmission of SCWL phytoplasma to the host plants was 11–12 min, with a minimum duration of salivation into phloem of 1.35 min. The female vectors had higher SCWL phytoplasma copy numbers than the male vectors, and displayed faster AAP, IAP, and LP. Overall, our findings provide important information related to the feeding behaviour of M. hiroglyphicus and its effect on the transmission of SCWL phytoplasma.  相似文献   

5.
Lumicella rotundata gen. et sp. n. is described based on specimens from Fujian Province, China. Habitus photos and illustrations of male genitalia of this new species are provided. Differences between the new genus and closely related genera are discussed.  相似文献   

6.
Abstract  Balclutha incisa is a widely distributed leafhopper common on grasses. In Perth, Western Australia, crab grass ( Digitaria sanguinalis ) and veldt grass ( Ehrharta longiflora ) were identified as host plants. Mating and subsequent oviposition occurred in late summer to early winter (August) on the panicles and stems of crab grass. We describe the biology and phenology of B. incisa within a restricted habitat over a 2-year period and we comment on oviposition sites, eggs and emergence of B. incisa . We describe the behaviour of two main predators, the Pacific damsel bug, Nabis kinbergii Reuter and muscid fly belonging to the genus Coenosia Meigen , particularly in regard to their response to visual and auditory signals produced by the leafhoppers.  相似文献   

7.
Chrysanthemum yellows (CY) phytoplasma has been transmitted with three leafhopper species: Euscelidius variegatus (Kirschbaum), Macrosteles quadripunctulatus (Kirschbaum) and Euscelis incisus (Kirschbaum): the first two species are reported as CY phytoplasma vectors for the first time. Leafhoppers were allowed to acquire the pathogen from the following source plants: Apium graveolens L., Catharanthus roseus L., Chrysanthemum carinatum Schousboe L. and C. frutescens L. DNA extracted from healthy or inoculative leafhoppers-exposed plants were analyzed by dot-blot and Southern hybridizations with a molecular probe constructed onto a fragment of European aster yellows phytoplasma DNA. The three leafhopper species were able to transmit CY phytoplasma after acquisition on chrysanthemum, but only M. quadripunctulatus and E. variegatus transmitted after feeding on periwinkle, and none acquired it from celery. All plant species tested were susceptible to CY, but while chrysanthemum and periwinkle were suitable for both inoculation and acquisition, celery did not seem to be a good source of phytoplasma for further inoculations. It is concluded that host plants influence leafhoppers' vectoring ability, possibly due to the different feeding behaviour of the insects on diverse plant species. Since CY, like several other phytoplasmas, can be transmitted by different insect species, it is likely that a close transmission specificity probably does not exist between phytoplasmas and their leafhopper vectors.  相似文献   

8.
Abstract:  The leafhopper Erythroneura vulnerata Fitch is native to North America, where it infests wild and cultivated grapes. In July 2004, E. vulnerata was recorded for the first time on Vitis vinifera L. (cv. Cabernet Sauvignon) in north-eastern Italy (Veneto region). This record is assumed to be the first in Europe. Preliminary observations on the pest distribution, seasonal abundance and the extent of symptoms in north-eastern Italy are reported.  相似文献   

9.
条大叶蝉属二新种记述(同翅目:大叶蝉科)   总被引:4,自引:0,他引:4  
本描述条大叶蝉属(Atkinsoniella Distant)2新种:瘤突条大叶蝉Atkinsoniella tylata sp.nov和黑翅条大叶蝉Atkinsoniella nigripennis sp.nov。模式标本保存在贵州大学昆虫研究所。  相似文献   

10.
记述采自四川的条大叶蝉属二新种:中带条大叶蝉Atkinsoniella mediofasciola sp. nov. 和叉端条大叶蝉A. furcula sp. nov.。模式标本保存在贵州大学农学院昆虫研究所。  相似文献   

11.
中国窗翅叶蝉属三新种和-新纪录种(同翅目:大叶蝉科)   总被引:3,自引:0,他引:3  
记述窗翅叶蝉属3新种:枝茎窗翅叶蝉Mileewa branchiuma sp.nov.、黑尾窗翅叶蝉M.nigricauda sp.nov.和褐点窗翅叶蝉M.mira sp.nov..对它们的形态特征和雄性外生殖器构造作了详细描述,并附主要特征图.中国新纪录种:乌苏窗翅叶蝉M.ussurica Anufriev在本文中记载.  相似文献   

12.
贵州斑大叶蝉属三新种(同翅目:大叶蝉科)   总被引:2,自引:0,他引:2  
沪述斑大叶蝉属Anatkina Yorng3新种,即蓝斑大叶蝉A.livimacula sp.nov.;红翅政大叶蝉A.rubipennis sp.nov.;双条斑大叶蝉A.bistriata sp.nov.,模式标本保存在贵州大学农学院。  相似文献   

13.
作为杆叶蝉亚科分类订正研究的一部分,本文依据捷克Moravian 博物馆收藏的Karasekia 属的模式标本和定名标本及英国自然博物馆(BM)馆藏定名标本对该属20世纪初记述的该属2个已知种作了再描记、绘出了形态特征图,并补充了属征。所有研究标本均归还原所属博物馆保藏。 巨索杆蝉属Karasekia Melichar Karasekia Melichar, 1912c117. 模式种 巨索杆蝉Karasekia lata (Melichar)。 分布 非洲。 1.巨索杆蝉Karasekia lata (Melichar) (图1) Wolfella lata Melichar,1905a299. Karasekia lata (Melichar), Melichar,1912c118. 分布 Tanganyika, 非洲。 观察标本 1♀ (正模,无腹部和前胸背板), Amani, 1904-Ⅲ-23, L. Melichar 采;1♂, Maherangulu, 东非;标本均借自 Moravian Museum, Brno。 2.冠齿巨索杆蝉Karasekia pugionata Melichar (图2) Karasekia pugionata Melichar, 1912c118. 分布Tanganyika, 肯尼亚,非洲。 观察标本 2♀♀(全模标本,MM),Maherangulu, 东非,L.Melichar 采。 1♂( ?, 无腹部末端), 1♀(BM,1961-343), 肯尼亚Emali Range, Sultan Hamud, (1493 m~1798 m), P. E. S.和 E. M. Whalley采;2♀♀(BM,1961-343),肯尼亚Wandanyi (1524 m),P. E. S. 和E. M. Whalley采;W. Knight鉴定。  相似文献   

14.
记述扁叶蝉属2新种,黄斑扁叶蝉Penthimia fulviguttata sp.nov和茂兰扁叶蝉Penthimia maolanensis sp.nov,模式标本保存在贵州大学昆虫研究所。  相似文献   

15.
Leafhoppers (Homoptera: Cicadellidae) possess epi-and hypopharyngeal chemosensilla within the head. In this paper, we describe the behaviors of leafhoppers (Graphocephala atropunctata Signoret) which have had two of the four main nerves to these sensilla cut. Insects with severed nerves (treated) and control insects (both sham-operated and normal) were offered a choice of two small mustard leaves infused with either distilled water or a 5% sucrose solution. The control insects showed a preference for the 5% sucrose leaf, whereas the treated leafhoppers did not; they distributed themselves on both leaves with similar frequency. Thus, severing the nerves of only half of the precibarial chemosensilla (epipharyngeal organ) resulted in major changes in leafhopper feeding behavior. This evidence supports the hypothesis that the precibarial chemosensilla mediate gustatory discrimination of chemical compounds within the plant.
Résumé Les cicadelles (Homoptera) possèdent dans la tête des sensilles gustatives sur l'épi et l'hypopharynx. Cette note décrit le comportement de Graphocephala atropunctata Signoret dont deux des quatre principaux nerfs de ces sensilles ont été coupés. Le choix entre des petites feuilles de moutarde infusées, soit dans de l'eau distillée, soit dans une solution de sucrose à 5%, a été proposé à ces insectes et à deux catégories de témoins, les uns intacts, les autres ayant subi une opération à blanc. Les témoins ont montré une préférence pour les feuilles traitées au sucrose, mais pas les cicadelles aux nerfs sectionnés. Les insectes sectionnés ont perforé les deux types de feuilles avec les mêmes fréquences. Ainsi la section de la moitié seulement des nerfs des sensilles gustatives précibariales (organe épipharyngien) a induit un changement fondamental du comportement alimentaire des cicadelles. Cette expérience appuie l'hypothèse selon laquelle les sensilles précibariales fournissent la discrimination gustative des composés chimiques de la plante.
  相似文献   

16.
记述坦小叶蝉属9新种,中国1新纪录种和1新组合,确认了1新异名,编制了中国坦小叶蝉属种检索表。模式标本均保存于西北农林科技大学昆虫博物馆。  相似文献   

17.
福建武夷山条大叶蝉属一新种(同翅目:大叶蝉科)   总被引:1,自引:2,他引:1  
本文记述条大叶蝉属1新种:指突条大叶蝉Atkinsoniella dactylia Yang et Li,sp.nov.。文中详细描记了新种的外部形态牲和雄虫外生殖器构造,并附主要特征图。  相似文献   

18.
    
The incidence and transmissibility of Flavescence dorée phytoplasma (FDP) in populations of the vector Scaphoideus titanus Ball (Homoptera: Cicadellidae) were investigated by periodically collecting nymphs and adults of the leafhopper species in four vineyards with high incidence of Flavescence dorée (FD)‐diseased grapevines. Insects were tested individually for FDP with an ELISA procedure, after transmission assays to broadbean seedlings and further transmission to grapevine cuttings. No transmission occurred when early or middle instar nymphs were used to inoculate broadbeans, although a limited number of fifth‐instar nymphs and young adults transmitted the pathogen to broadbean seedlings. However, the same batches of insects transmitted FDP more efficiently to grapevine cuttings during prolonged inoculation periods, confirming the existence of a latent period before infected insects become infective. The proportions of ELISA‐positive individuals in the three categories of insects used for transmission assays reflected the rate of FDP transmission to grapevine cuttings. According to the data obtained by ELISA and from field sampling of first‐instar nymphs, we adapted the proportions of nymph hatching, of infected leafhoppers, and of infective leafhoppers (assuming a conservative latent period in the vector of 30 days) to logistic models as a function of degree‐days. We then discussed the possible use of the model developed for improving vector control decisions in FD‐infected vineyards.  相似文献   

19.
XU Gui-lan 《Insect Science》2000,7(3):218-222
Abstract This paper deals with 3 new leaf hopper species of the genus Lodiana Nielson from China, i.e., Lodiana platyfasciata, L. harnularis and L. kuohi . Type specimens are deposited in Department of Plant Protection, Anhui Agricultural University.  相似文献   

20.
蔡平  江佳富 《昆虫学报》2000,43(4):413-416
记述了在中国云南省和贵州省采集的肖点耳叶蝉属Midoria Kato二新种:何氏肖点耳叶蝉M.hei新种和环突肖点耳叶蝉M.annulata新种,并对属征补充描记,附分种检索表。模式标本保存在安徽农业大学昆虫标本室。  相似文献   

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