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1.
This preliminary study was planned to investigate the effects of resveratrol on oxidative–nitrosative stress markers and on
trace element concentrations in blood and on circulatory system parameters in rats. Twenty-five Sprague–Dawley male rats,
10–12 weeks old, with mean body weight of 295 g were used in the study. Administration of resveratrol (0.5 ml/day) was performed
in experimental group in 10 days. In control (n = 10) and in experimental groups (n = 15), after 1 week training period, systolic arterial blood pressures and heart rates were recorded daily. At the end of
the tenth day, blood samples of control and experimental groups were drawn. Total nitrite, nitrite, nitrate, malondialdehyde,
copper, zinc concentrations in plasma, superoxide dismutase, and catalase activities and copper, zinc concentrations in red
cell were determined both in control and experimental groups. Alterations in oxidative and nitrosative stress markers, trace
element concentrations, and circulatory system parameters in experimental group compared to controls were observed. The results
of this study were discussed according to the effect of resveratrol.
This study was presented at “The 5th International Congress of Pathophysiology (ISP2006)” June 28–July 1, 2006 Beijing, China. 相似文献
2.
Mursel Bicer Mehmet Gunay Mustafa Akil Mustafa Cihat Avunduk Rasim Mogulkoc Abdulkerim Kasim Baltaci 《Biological trace element research》2011,139(3):317-324
This study aims to examine the effect of zinc administration on liver glycogen levels of rats in which diabetes was induced
with streptozotocin and which were subjected to acute swimming exercise. The study was conducted on 80 adult Sprague–Dawley
male rats, which were equally allocated to eight groups: group 1, general control; group 2, zinc-administrated control; group
3, zinc-administrated diabetic control; group 4, swimming control; group 5, zinc-administrated swimming; group 6, zinc-administrated
diabetic swimming; group 7, diabetic swimming; group 8, diabetic control group. In order to induce diabetes, animals were
injected with 40 mg/kg intraperitoneal (ip) streptozotocin. The injections were repeated in the same dose after 24 h. Animals
which had blood glucose at or above 300 mg/dl 6 days after the last injections were accepted as diabetic. Zinc was administrated
ip for 4 weeks as 6 mg/kg/day per rat. Hepatic tissue samples taken from the animals at the end of the study were fixed in
95% ethyl alcohol. Cross sections of 5 μm thickness, taken by the help of a microtome from the tissue samples buried in paraffin,
were placed on a microscope slide and stained with periodic acid–Schiff and evaluated by light microscope. All microscopic
images were transferred to a PC and assessed with the help of Clemex PE3.5 image analysis software. The lowest liver glycogen
levels in the study were obtained in groups 3, 4, 6, 7, and 8. Liver glycogen levels in group 5 were higher than groups 3,
4, 6, 7, and 8, but lower than groups 1 and 2 (p < 0.05). Groups 1 and 2 had the highest liver glycogen levels. The results obtained from the study indicate that liver glycogen
levels which dropped in acute swimming exercise were restored by zinc administration and that diabetes induced in rats prevented
the protective effect of zinc. 相似文献
3.
Effect of an inhibitor of nitric oxide production on Cu−Zn/SOD and its cofactors in diabetic rats 总被引:1,自引:0,他引:1
The aim of this study was to investigate the effect of an inhibitor of nitric oxide production, N(omega)-nitro-L-arginine methyl esther (L-NAME) on Cu-Zn/SOD (superoxide dismutase) enzyme activity and copper and zinc concentrations in diabetes-induced rats. The control group consisted of 12 male albino Sprague-Dawley rats, 10-12 wk of age and weighing 300 g. Twenty-six albino Sprague-Dawley rats, 10-12 wk of age and weighing 315 g, constituted the experimental group. The experimental group was divided into two groups. The first group (n=12) constituted streptozotocininduced (55 mg/kg, intraperitoneally) diabetic rats and the second group (n=14) was administered L-NAME (1 mg/kg/d) after streptozotocin induction. For determination of Cu-Zn/SOD activity, spectrophotometry was used. Zinc and copper concentrations were determined by atomic absorption spectrophotometry. Results showed that Cu-Zn/SOD activity was increased significantly in both experimental groups compared to controls, and the increase in the second group was higher than in the first group (p<0.01, p<0.01, p<0.05). Plazma zinc concentration was increased in the second group when compared with controls (p<0.05). Plasma copper was decreased significantly in the second group compared to controls and the first group (p<0.001, p<0.001). Red cell copper concentration was decreased significantly in the first group compared to controls (p<0.05). This study showed that L-NAME administration has ensured an additive effect on the antioxidant defense system, which was proved by the increase in Cu-Zn/SOD activity. This increase might have a protective effect against tissue damage in the acute period, with corresponding changes in zinc and copper concentrations. 相似文献
4.
This study assessed the impact of either cadmium chloride (Cd) or sodium selenite (Se) alone or in combination on male Sprague–Dawley
rats. For this purpose, body and liver weights, comet and TUNEL assays, histological analysis and levels of lipid peroxidation
and antioxidants in liver were determined in four groups of male Sprague–Dawley rats. The rats were given subcutaneous doses
of 1 mg/kg body weight (BW) of either normal saline (control = Ct) or Cd or Se or Cd plus Se (Cd + Se) on alternate days for
4 weeks. The Cd group showed increased DNA damage, apoptosis and hepatic levels of lipid peroxidation and altered histology.
Conversely, the antioxidant levels in this group were decreased as compared with the control group. The Se group also showed
DNA damage, apoptosis and altered histology and reduced catalase activity, but it was less severe than the Cd group. In the
Cd + Se group, ameliorating effects of Se on Cd-induced changes were observed. While the Se was able to curtail the toxic
effect of Cd, the Cd or Se alone were genotoxic and cytotoxic for rats receiving a high pharmacological but non-fatal dose
of 1 mg/kg BW. 相似文献
5.
Abdulkerim Kasim Baltaci Hakki Gokbel Rasim Mogulkoc Nilsel Okudan Kagan Ucok Ihsan Halifeoglu 《Biological trace element research》2010,134(1):79-83
The objective of the present study was to determine the effects of exercise and zinc deficiency on some elements in rats.
Forty adult male Sprague–Dawley species male rats were allocated to four groups as follows: Group 1: control, Group 2: zinc-deficient,
Group 3: exercise in which exercise group fed with a normal diet, Group 4: zinc-deficient exercise, exercise group fed by
a zinc-deficient diet for 15 days. After the procedure ended, rats in groups 3 and 4 were exercised on the treadmill for 60 min
at a speed of 6 m/min until the exhaustion. The rats were decapitated 48 h after exercise together with their controls, and
blood samples were collected to determine copper (Cu), iron (Fe), magnesium (Mg), calcium (Ca), and phosphorus (P) levels.
The highest Cu and Fe values in the serum were obtained in group 2 (p < 0.01). The levels of these elements in group 4 were lower than those in group 2 and higher than the levels in groups 1
and 3 (p < 0.01). Serum Mg levels did not differ significantly between groups. Group 4 had the lowest serum Ca and P levels (p < 0.01). These same parameters in Group 2 were higher than those in group 4 but significantly lower than those in groups
1 and 3 (p < 0.01). There was no significant difference between Ca and P levels of groups 1 and 3. The results of the study indicate
that zinc deficiency adversely affects copper, iron, calcium, and phosphorus mechanisms and that these adverse effects much
more marked after an effort exercise. 相似文献
6.
Ayhan Dogukan Nurhan Sahin Mehmet Tuzcu Vijaya Juturu Cemal Orhan Muhittin Onderci James Komorowski Kazim Sahin 《Biological trace element research》2009,131(2):124-132
The present study was conducted to investigate the effects of chromium histidinate (CrHis) against experimentally induced
type II diabetes and on chromium (Cr), zinc (Zn), selenium (Se), manganese (Mn), iron (Fe), and copper (Cu) in serum, liver,
and kidney of diabetic rats. The male Wistar rats (n = 60, 8 weeks old) were divided into four groups. Group I received a standard diet (12% of calories as fat); group II were
fed standard diet and received CrHis (110 mcg CrHis/kg body weight per day); group III received a high-fat diet (HFD; 40% of calories as fat) for 2 weeks and then
were injected with streptozotocin (STZ) on day 14 (STZ, 40 mg/kg i.p.; HFD/STZ); group IV were treated as group III (HFD/STZ)
but supplemented with 110 mcg CrHis/kg body weight per day. The mineral concentrations in the serum and tissue were determined
by atomic absorption spectrometry. Compared to the HFD/STZ group, CrHis significantly increased body weight and reduced blood
glucose in diabetic rats (p < 0.001). Concentrations of Cr, Zn, Se, and Mn in serum, liver, and kidney of the diabetic rats were significantly lower
than in the control rats (p < 0.0001). In contrast, higher Fe and Cu levels were found in serum and tissues from diabetic versus the non-diabetic rats
(p < 0.001). Chromium histidinate supplementation increased serum, liver, and kidney concentrations of Cr and Zn both in diabetic
and non-diabetic rats (p < 0.001). Chromium supplementation increased Mn and Se levels in diabetic rats (p < 0.001); however, it decreased Cu levels in STZ-treated group (p < 0.001). Chromium histidinate supplementation did not affect Fe levels in both groups (p > 0.05). The results of the present study conclude that supplementing Cr to the diet of diabetic rats influences serum and
tissue Cr, Zn, Se, Mn, and Cu concentrations. 相似文献
7.
Bicer M Akil M Sivrikaya A Kara E Baltaci AK Mogulkoc R 《Journal of physiology and biochemistry》2011,67(4):511-517
The present study aims to examine the effect of supplementation of zinc on the distribution of various elements in the sera
of diabetic rats subjected to an acute swimming exercise. A total of 80 Sprague–Dawley-type adult male rats were equally allocated
to one of eight groups: Group 1, general; Group 2, zinc-supplemented; Group 3, zinc-supplemented diabetic; Group 4, swimming
control; Group 5, zinc-supplemented swimming; Group 6, zinc-supplemented diabetic swimming; Group 7, diabetic swimming; and
Group 8, diabetes. The rats were injected with 40 mg/kg/day subcutaneous streptozotocin (STZ) twice, with a 24-h interval
between two injections. Zinc was supplemented at a dose of 6 mg/kg/day (ip) for 4 weeks. Blood samples were collected at the
end of the 4-week study, and serum levels of lead, cobalt, molybdenum, chrome, sulfur, magnesium, manganese, sodium, potassium,
phosphorus, copper, iron, calcium, zinc, and selenium (mg/L) were determined with atomic emission. The lowest molybdenum,
chrome, copper, iron, potassium, magnesium, sodium, phosphorus, lead, selenium, and zinc values were obtained in Group 7 and
8. These same parameters were higher in the swimming exercise group (Group 4), relative to all other groups. The values in
zinc-supplemented groups were found lower than the values in Group 4, but higher than those in Group 6 and 7. The results
obtained from the study demonstrate that acute swimming exercise and diabetes affect the distribution of various elements
in the serum, while zinc supplementation can prevent the negative conditions associated with both exercise and diabetes. 相似文献
8.
Souvik Roy Sudheer Kumar Dontamalla Anil Kumar Mondru Santanu Sannigrahi Prabhakar Reddy Veerareddy 《Biological trace element research》2011,139(1):55-71
To investigate whether sodium selenate treatment would impact on the onset of diabetic nephropathy, we examined blood glucose,
serum biochemical components, and interrelationship between oxidative stress, TGF-β1, and apoptosis in streptozotocin (STZ)
induced diabetic rats. Sixty male Wistar rats were divided into six groups. Group I (n = 10), normal control; Group II (n = 10), diabetic control; Group III (n = 10), sodium selenate (16 μmoles/kg) + diabetic; Group IV (n = 10), sodium selenate (32 μmoles/kg) + diabetic; Group V (n = 10), sodium selenate (16 μmoles/kg) control; and Group VI (n = 10), sodium selenate (32 μmoles/kg) control. Sodium selenate was administered via orogastric route for 10 weeks. In the
diabetic group, diabetes was induced by single intraperitoneal injection of STZ (50 mg/kg). The levels of blood glucose were
estimated and total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, creatinine, urea, and albumin
were detected in serum. Antioxidant status was examined by measuring the superoxide dismutase (SOD), catalase, glutathione,
and lipid peroxidation in kidney tissues. Histopathological studies were performed in the kidney tissue sections. The expression
of TGF-β1 was estimated by the immunohistochemical analysis in kidneys. Apoptotic study in kidney was performed using the
TdT-mediated dUTP nick end labeling technique. It was observed that blood glucose, serum, total cholesterol, HDL cholesterol,
triglycerides, creatinine, urea, and albumin were significantly higher in diabetic control groups. Diabetic + sodium selenate
(16 and 32 μmoles/kg) significantly reduced blood glucose, serum, total cholesterol, HDL cholesterol, triglycerides, creatinine,
urea, and albumin levels. Selenium-treated groups significantly increased antioxidant enzyme activities (SOD, catalase, and
glutathione) in kidneys of diabetic rats. All enzyme activities of selenium control groups did not differ compared with the
normal control. Sodium selenate reduces significantly lipid peroxidation in diabetic rats. Cellular architecture of the diabetic
rats was altered whereas sodium selenate administration rectifies the degenerative changes of the kidney. Profound immunopositivity
of TGF-β1 was observed in the glomerular and tubulointerstitial cells of diabetic rat kidney. Immunopositivity of TGF-β1 was
significantly reduced in both low and high dose of sodium-selenate-treated rats (P < 0.05, P < 0.01). High numbers of apoptotic cells were observed in diabetic rats whereas sodium selenate in both doses significantly
reduces the incidence of apoptosis (P < 0.05, P < 0.01). We conclude herein that sodium selenate has the potential to play a significant role in limiting the renal impairment
by altering the apoptosis and TGF-β1 in experimental diabetic rats. 相似文献
9.
Chae CH Jung SL An SH Jung CK Nam SN Kim HT 《Journal of physiology and biochemistry》2011,67(2):235-241
We investigated the effects of treadmill exercise performed regularly for 6 weeks on the levels of nerve growth factor (NGF),
tyrosine kinase A and p75 receptors, phosphatidylinositol 3-kinase (PI3-K), mitogen-activated protein kinase/extracellular
signal-regulated kinase (Erk) 1,2, cyclic AMP response element-binding protein (CREB), and caspase-3 in the soleus of rats
with streptozotocin (STZ)-induced diabetes. Thirty-two male Sprague–Dawley rats were divided into the following four groups:
(1) normal control group (NCG; n = 8), (2) normal exercise group (NEG; n = 8), (3) diabetes control group (DCG; n = 8), and (4) diabetes exercise group (DEG; n = 8). Diabetes was induced by intraperitoneal injection of STZ (55 mg/kg dissolved in 0.05 M citrate buffer, pH 4.5). Rats
were subjected to treadmill exercise 5 days a week for 6 weeks. The protein level of NGF significantly increased in the NEG
and DEG (p < 0.001), whereas the levels of tyrosine kinase A and p75 receptors significantly increased in the NEG (p < 0.001). The levels of t-PI3-K, p-PI3-K, and p-CREB, and the p-CREB/t-CREB ratio significantly increased in the NEG (p < 0.001, respectively). The p-PI3-K/t-PI3-K ratio significantly increased in the DEG (p < 0.001). The p-Erk1/t-Erk1 ratio significantly increased in the NEG (p < 0.001), whereas the p-Erk2/t-Erk2 ratio significantly decreased in the DCG and DEG (p < 0.001). The caspase-3 level significantly increased in the DCG compared with that in the DEG (p < 0.001). These results suggest that treadmill exercise increases NGF levels and accelerates p-PI3-K activation in order
to suppress apoptotic cell death in the soleus muscle of diabetic rats. 相似文献
10.
We investigated the effect of 17β-estradiol (E2) alone and separately vitamin E treatment on trace element status of rats following an ovariectomic operation. Forty rats
were equally divided into four groups: Group 1, control, non-ovariectomized rats; Group 2, (OVX) rats, ovariectomized under
general anesthesia; Group 3, (OVX+E2) rats, the group received a 40 μg kg−1 subcutan dose of E2 per day after ovariectomy; and Group 4, (OVX + E2 + vitamin E) rats, received the same E2 treatment, but with an additional 100 mg kg−1 intraperitoneal dose of vitamin E per day after ovariectomy. At the end of the 30-day experiment, the rats were sacrificed
and their blood was collected for the measurement of zinc, copper, iron, phosphorus, selenium, magnesium, calcium, manganese,
and chromium; copper–zinc superoxide dismutase (SOD); manganese-superoxide dismutase (Mn-SOD); glutathione peroxidase (Se-GSH-Px);
and catalase (CAT). The levels of zinc, copper, iron, phosphorus, selenium, calcium, chromium, and manganese and activities
of SOD, Mn-SOD, Se-GSH-Px, and CAT were lower in the OVX than in the control group, but magnesium level was unaffected. However,
zinc, copper, iron, phosphorus, selenium, calcium, chromium, and manganese levels and SOD, Mn-SOD, Se-GSH-Px, and CAT activities
were higher under separate E2 and E2 + vitamin E treatments. The level of magnesium in the treated-OVX groups was not different than in the OVX group. In conclusion,
E2 treatment has an ameliorating effect on the trace element status in OVX, and this effect may be enhanced with the addition
of vitamin E. 相似文献
11.
The serum concentrations of copper, zinc, iron, and cobalt and copper/zinc ratio were investigated in horses infected with
equine herpesvirus-1 (EHV-1). Nine horses were naturally infected with the virus and nine healthy horses served as controls.
The concentrations of copper, zinc, iron, and cobalt were determined spectrophotometrically in the blood serum of all horses.
The results were (expressed in micrograms per deciliters) copper 2.80 ± 0.34 vs 1.12 ± 0.44, zinc 3.05 ± 0.18 vs 0.83 ± 0.06,
iron 2.76 ± 0.17 vs 3.71 ± 0.69, cobalt 0.19 ± 0.37 vs 0.22 ± 0.45, and copper/zinc ratio 0.72 ± 0.38 vs 1.41 ± 0.36 for control
vs infected group, respectively. In conclusion, copper and zinc concentrations of the infected group were lower than the control
group (p < 0.001), whereas iron concentration and the copper/zinc ratio of the infected group were higher than the control group (p < 0.05 and p < 0.001). The cobalt concentration was not found to be statistically different between two groups. It might be emphasized
that copper/zinc ratio was significantly affected by the EHV-1 infection, so it could be taken into consideration during the
course of infection.
An erratum to this article can be found at 相似文献
12.
We previously reported that reduced platelet endogenous antioxidant enzymes activities are related to the low plasma zinc
level in patients with end-stage renal failure (ESRF). In this study, we attempt to evaluate whether dietary zinc deprivation
reduces the activities of endogenous antioxidant and then enhances oxidative stress in the unstimulated platelet of normal
and 5/6 nephrectomized (Nx) rats because increased platelet oxidative stress is suggested to involve in the incidence of thrombotic
and atherosclerotic diseases. Male Sprague–Dawley rats (n = 48) were fed a zinc-deficient diet and deionized distilled water for 1 week to induce reduction of plasma zinc level. Half
of the rats continued on this diet for 4 weeks as zinc-deplete group, and the other half were maintained on the same diet
but with zinc-supplemented water (120 mg/L zinc sulfate solution) to correct the reduction of plasma zinc level as zinc-replete
group. Half of each group underwent 5/6 Nx, while the other half underwent sham operation. Another 12 normal rats were fed
standard rat chow (containing 23.4% protein and 50 ppm zinc) and drank deionized distilled water as normal control rats. In
zinc-deplete rats including sham-operated and 5/6 Nx rats exhibited lower endogenous antioxidant enzymes activities such as
reduced glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GPX) and higher malondialdehyde (MDA) levels
than normal control rats in the unstimulated platelets. However, in zinc-replete rats including sham-operated and 5/6 Nx rats
have a normal endogenous antioxidant enzymes activity and normal MDA levels in the unstimulated platelets. We suggest that
in uremia, the low plasma zinc level may be a risk factor for thrombotic and atherosclerotic diseases because it reduces the
activities of endogenous antioxidant enzymes and increases oxidative stress in the unstimulated platelet.
Supported by grant 92-117 from Taipei Veterans General Hospital 相似文献
13.
The objective of this study is to investigate the neurotoxicity of drinking water fluorosis on rat hippocampus. Just weaning
male Sprague–Dawley rats were randomly divided into four groups and given 15, 30, and 60 mg/L NaF solution and distilled water,
respectively, for 9 months. The fluidity of brain synaptic membrane and expression level of postsynaptic density 95 (PSD-95)
were tested. Results showed that the fluidity of brain synaptic membrane decreased gradually with increasing of fluoride concentration,
and it was significantly decreased (P < 0.05) in moderate-fluoride group compared with control group, and expression level of PSD-95 was significantly decreased
(P < 0.01) in moderate-fluoride group when compared with that of control group. These results indicate that decrease of synaptic
membrane fluidity and PSD-95 expression level may be the molecular basis of central nervous system damage caused by fluoride
intoxication; PSD-95 in CA3 region of hippocampus is probably a target molecule for fluoride. 相似文献
14.
Salgueiro MJ Arnoldi S Kaliski MA Torti H Messeri E Weill R Zubillaga M Boccio J 《Biological trace element research》2009,127(2):143-147
The purpose of the present work was to evaluate the iron bioavailability of a new ferric pyrophosphate salt stabilized and
solubilized with glycine. The prophylactic–preventive test in rats, using ferrous sulfate as the reference standard, was applied
as the evaluating methodology both using water and yogurt as vehicles. Fifty female Sprague–Dawley rats weaned were randomized
into five different groups (group 1: FeSO4; group 2: pyr; group 3: FeSO4 + yogurt; group 4: pyr + yogurt and group 5: control). The iron bioavailability (BioFe) of each compound was calculated using
the formula proposed by Dutra-de-Oliveira et al. where BioFe % = (HbFef − HbFei) × 100/ToFeIn. Finally, the iron bioavailability
results of each iron source were also given as relative biological value (RBV) using ferrous sulfate as the reference standard.
The results showed that both BioFe % and RBV % of the new iron source tested is similar to that of the reference standard
independently of the vehicle employed for the fortification procedure (FeSO4 49.46 ± 12.0% and 100%; Pyr 52.66 ± 15.02% and 106%; FeSO4 + yogurth 54.39 ± 13.92% and 110%; Pyr + yogurt 61.97 ± 13.54% and 125%; Control 25.30 ± 6.60, p < 0.05). Therefore, the stabilized and soluble ferric pyrophosphate may be considered as an optimal iron source for food
fortification. 相似文献
15.
Mohamed A. Haidara Mohamed D. Morsy Hesham A. Abdel-Razek Dimitri P. Mikhailidis Esma R. Isenovic 《Journal of physiology and biochemistry》2010,66(3):255-264
Septicemia leads to oxidative stress with overproduction of reactive-oxygen species (ROS) and consumption of endogenous antioxidant
enzymes. We tested a twofold hypothesis: (1) does oxidative stress (OxS) induced by sepsis acting alone or in concert with
augmented inflammatory processes contributes to sepsis-related vascular dysfunction, and, (2) whether ozone (O3) and l-canavanine (CAV) mitigate the negative impact of the aforementioned phenomena. We investigated the relative impact of treatment
with CAV and/or O3 on vascular OxS associated vascular functional changes in septicemic rats. For this study, 60 male Sprague–Dawley rats were
used and divided into six experimental groups (n = 10): control group (C), sham-operated (Sham), septicemic rats (S), S rats treated with CAV (100 mg/kg. i.p; S + CAV), S
rats treated with O3 (1.2 mg/kg, i.p.; S + O3) and S rats treated with both O3 and CAV (S + O3 + CAV). After 22 h, the mean arterial blood pressure (MAP), the aortic ring vascular reactivity to phenylephrine, abdominal
aortic blood flow (AABF), serum tumor necrosis factor-α (TNF-α) and plasma nitrite/nitrate (NOx) concentration were measured.
In addition, hepatic antioxidant enzyme activities sodium dismutase (SOD) and glutathione peroxidase (GSH-Px) were estimated.
Septicemia caused significant elevation of serum TNF-α (p < 0.001) and plasma NOx (p < 0.001) and significant (p < 0.001) reduction of AABF (p < 0.001), aortic vascular response to phenylephrine (p < 0.001), MAP (p < 0.001) and hepatic SOD and GSH-Px activity (p < 0.001) compared with the C group, while treatment with O3 and/or CAV induced significant amelioration of all those increases. Abnormalities were attenuated to a similar extent with
treatment with both O3 and CAV. These results suggested that concomitant administration of O3 and CAV alleviated the compromised vascular reactivity in septicemic conditions and prevent its progression into septic shock
compared with each alone. 相似文献
16.
Alireza Nakhaee Mohammad Bokaeian Azim Akbarzadeh Mohammad Hashemi 《Biological trace element research》2010,136(2):221-231
High blood glucose concentration in diabetes induces free radical production and, thus, causes oxidative stress. Damage of
cellular structures by free radicals play an important role in development of diabetic complications. In this study, we evaluated
effects of sodium tungstate on enzymatic and nonenzymatic markers of oxidative stress in brain of streptozotocin (STZ)-induced
diabetic rats. Rats were divided into four groups (ten rats in each group): untreated control, sodium tungstate-treated control,
untreated diabetic, and sodium tungstate-treated diabetic. Diabetes was induced with an intraperitoneal STZ injection (65 mg/kg
body weight), and sodium tungstate with concentration of 2 g/L was added to drinking water of treated animals for 4 weeks.
Diabetes caused a significant increase in the brain thiobarbituric acid reactive substances (P < 0.01) and protein carbonyl levels (P < 0.01) and a decrease in ferric reducing antioxidant power (P < 0.01). Moreover, diabetic rats presented a reduction in brain glucose-6-phosphate dehydrogenase (21%), superoxide dismutase
(41%), glutathione peroxidase (19%), and glutathione reductase (36%) activities. Sodium tungstate reduced the hyperglycemia
and restored the diabetes-induced changes in all mentioned markers of oxidative stress. However, catalase activity was not
significantly affected by diabetes (P = 0.4), while sodium tungstate caused a significant increase in enzyme activity of treated animals (P < 0.05). Data of present study indicated that sodium tungstate can ameliorate brain oxidative stress in STZ-induced diabetic
rats, probably by reducing of the high glucose-induced oxidative stress and/or increasing of the antioxidant defense mechanisms. 相似文献
17.
Hypertension: does impaired endothelium-dependent relaxation affect superoxide scavenging? 总被引:3,自引:0,他引:3
The purpose of this study was to investigate the effects of acute nitric oxide synthase inhibition on mean arterial blood
pressure, oxidative stress markers such as plasma malondialdehyde (MDA) concentration, intracellular antioxidant enzyme activities
such as copper-zinc superoxide dismutase (Cu/Zn SOD) and catalase and on trace elements important for activity and stability
of Cu/Zn-SOD.
Wistar-Kyoto rats (approx 150 g) (n=11) were treated with N
ω-nitro-l-arginine methyl esther (l-NAME) (0.5 mg/mL) for 2 d. Age- and bodyweight-matched rats (n=10) were used for control group. Their systolic blood pressures and heart rates were recorded daily during the experimental
period and also before their blood samples were drawn. Plasma MDA, plasma and red cell zinc and copper concentrations, and
red cell Cu/Zn-SOD and catalase activities were determined.
A progressive rise in systolic arterial blood pressure was observed compared to the control group (p<0.001). The heart rate of the experimental group was reduced on the third day (p<0.05). Plasma MDA concentration and red cell catalase activity were increased in the experimental group (p<0.001 and p<0.001, respectively). Plasma copper and red cell zinc concentrations were also increased significantly in the experimental
group (p<0.001 and p<0.01, respectively).
In conclusion, impairment in endothelium-derived relaxation altered mean arterial blood pressure, oxidant status, and trace
element concentrations.
Presented at the Advanced Course (sponsored by NATO-ASI, SFRR, FEBS, UNESCO-MCBN, IUBMB) “Free Radicals, Nitric Oxide, and
Inflamation: Molecular, Biochemical, and Clinical Aspects,” Lara, Antalya, Turkey, September 23–October 3, 2001. 相似文献
18.
Mustafa Akil Umit Gurbuz Mursel Bicer Abdullah Sivrikaya Rasim Mogulkoc Abdulkerim Kasim Baltaci 《Biological trace element research》2011,142(3):651-659
The present study aims to evaluate the effect of selenium supplementation on lipid peroxidation and lactate levels in rats
subjected to acute swimming exercise. Thirty-two adult male rats of Sprague–Dawley type were divided into four groups. Group
1, control; group 2, selenium-supplemented; group 3, swimming control; group 4, selenium-supplemented swimming group. The
animals in groups 2 and 4 were supplemented with (i.p.) 6 mg/kg/day sodium selenite for 4 weeks. The blood samples taken from
the animals by decapitation method were analyzed in terms of erythrocyte-reduced glutathione (GSH), serum glutathione peroxidase
(GPx) and superoxide dismutase (SOD), and plasma malondialdehyde (MDA) and lactate using the colorimetric method, and serum
selenium values using an atomic emission device. In the study, the highest MDA and lactate values were found in group 3, while
the highest GSH, GPx and SOD values were obtained in group 4 (p < 0,001). Group 2 had the highest and group 3 had the lowest selenium levels (p < 0,001). Results of the study indicate that the increase in free radical production and lactate levels due to acute swimming
exercise in rats might be offset by selenium supplementation. Selenium supplementation may be important in that it supports
the antioxidant system in physical activity. 相似文献
19.
Gümüş M Yüksel H Evliyaoğlu O Kapan M Böyük A Önder A Aldemir M 《Biological trace element research》2011,143(1):386-393
Ellagic acid (EA) is a natural polyphenolic compound. Although, modulator effects of EA on copper (Cu) and zinc (Zn) levels
in some liver diseases have been reported in experimental animals, its effects in obstructive jaundice (OJ) has not been clarified.
We aimed to evaluate potential effects of EA on Cu and Zn levels in liver and serum of cholestatic rats. Forty Wistar albino
rats were equally divided into four groups. First group was used as controls. Second group received EA (60 mg−1 kg−1 day−1) for 8 days. Third was OJ group, and fourth group was OJ plus EA group. After 8 days, blood and liver samples were obtained.
Higher serum and liver Cu and lower serum and liver Zn levels were found in OJ group (p < 0.05) compared with other groups. However, these differences reached to significant levels for Cu in serum and for Zn in
lever. Higher serum copper levels were decreased, and lower liver Zn levels were increased by EA treatment in cholestatic
rats (p < 0.05). Also, higher Cu/Zn ratio in OJ group was decreased by EA treatment both in liver (p < 0.05) and in serum (p < 0.05). Significantly higher serum bilirubin, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase
values were found in OJ and OJ + EA groups compared with the control and EA groups (p < 0.05). In conclusion, result of the current study indicated that ellagic acid has modulator effects on Cu and Zn levels
in liver and serum of cholestatic rats. 相似文献
20.
Oxidative stress is considered to be the main cause of diabetic complications. In the current study, we investigated the effect
of selenium–vitamin E combination and melatonin on lipid peroxidation (LPO) and scavenging enzyme activity in the blood of
streptozocin (STZ)-induced diabetic pregnant rats. Forty female Wistar rats were randomly divided into five groups. The first
and second groups were used as the non-pregnant control and pregnant control groups, respectively. The third group was the
pregnant diabetic group. Vitamin E plus selenium and melatonin were administered to the diabetic pregnant rats consisting
fourth and fifth groups, respectively. Diabetes was induced on day 0 of the study by STZ. Blood samples were taken from all
animals on the 20th day of pregnancy. LPO level was higher in diabetic pregnant rats than in control, although superoxide
dismutase, catalase, and glutathione peroxidase activities were lower in diabetic pregnant animals than in control. LPO levels
were lower both in the two treatment groups than in the diabetic pregnant rats, whereas selenium–vitamin E combination and
melatonin caused a significant increase in the activities of these antioxidant enzymes (p < 0.01). In conclusion, vitamin E plus selenium seems to be a more potent antioxidant compared to melatonin in diabetic pregnant
rats. Melatonin did not significantly affect the elevated glucose concentration of diabetic pregnant treated with melatonin
group. Vitamin E plus selenium may play a role in preventing diabetes-related diseases of pregnant subjects. 相似文献