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1.
Iwona Stroynowski Anders Orn Robert S. Goodenow Minnie McMillan James Forman Peter R. Brayton Jeffrey Frelingers Leroy Hood 《Immunogenetics》1984,20(2):141-154
We have shown that cytotoxic T lymphocytes (CTL) raised in H-2
dmice use H-2Ld but not H-2Dd or H-2Kd antigens as restricting elements in lymphocytic choriomeningitis virus (LCMV) and vesicular stomatis virus (VSV) infections. To localize the regions of H-2Ld protein recognized by CTL, we constructed a recombinant H-2L
d/D
dgene encoding a hybrid antigen with 1 and 2 external domains of H-2Ld and 3, transmembrane and cytoplasmic domains of H-2Dd. The recombinant gene was transfected into mouse cells and the hybrid molecules were characterized serologically, biochemically and functionally. In all assays, H-2Ld/Dd molecules were recognized by LCMV- and VSV-specific H-2Ld-restricted CTL in a manner similar to that of wild-type H-2Ld antigens. Analogous results were obtained with alloreactive CTL. Hybrid antigens containing the 3 domain of H-2Ld fused to 1 and 2 domains of a Qa-2,3 region-encoded antigen were not used as restricting elements by LCMV-specific CTL. These results suggest that H-2Ld-restricted CTL directed against LCMV and VSV recognize determinants controlled by the 1 and/or 2 domains of the H-2Ld molecule.Abbreviations used in this paper CTL
cytotoxic T lymphocytes
- VSV
vesicular stomatitis virus
- LCMV
lymphocytic choriomeningitis virus
- tk
thymidine kinase
- HAT
hypoxanthine, aminopterine, thymidine
- HSV
herpes simplex virus
- FCS
fetal calf serum
- SAC
Staphylococcus aureus Cowan I strain
- TM
transmembrane
- CYT
cytoplasmic 相似文献
2.
Wilhelm Schmidt Hilliard Festenstein Philip J. Ward Arnold R. Sanderson 《Immunogenetics》1981,13(6):483-491
Radiolabeled human 2-microglobulin (2m) can bind to mouse histocompatibility (H-2) antigens on the cell surface or to partially purified H-2 antigens in solution. The complexes containing human 2m and H-2 antigens from C3H (H-2k) mice could be immunoprecipitated specifically with alloantisera, rabbit anti-H-2 xenoantisera, and with monoclonal H-2-specific antibodies. Specific association with H-2 antigens was also observed with other haplotypes. The only exception was B10.D2 (H-2
d
) from which complexes containing human 2M could only be precipitated with anti-H-2 xenosera. Thus radiolabeled human 2M can be used as a specific label for mouse H-2 antigens in precipitation and radioimmunoassays. The application of this finding extends to major histocompatibility complex antigens of other species, and to differentiation antigens with primary association with 2m.Abbreviations used in this paper MHC
major histocompatibility complex
- 2m
2-microglobulin
- LcH
Lens culinaris hemagglutinin 相似文献
3.
Spleen cells from NZB mice make an unexpected primary cytotoxic T lymphocyte (CTL) response to BALB/c cells in vitro. In this study, it is shown that this response is comprised of at least three independent components. These include a response to antigens recognized in association with H-2d products, a response to Qa-1b-associated antigens which is notH-2-restricted and a response directed toward antigens not associated with either H-2d- or Qa-1b-coded determinants. The last response appears to be the weakest of the three. In addition, cells from NZB F1 mice which were either homozygous (Qa-1
a
/Qa-1
a
) or heterozygous (Qa-1
a
/Qa-1
b
) forQa-1 alleles, all responded to BALB/c cells. These data suggest that the NZB CTL response to BALB/c cells is not solely dependent on antigens coded for by genes in theH-2D-Tla region for either the sensitization or effector phases of the response. The ontogeny of the NZB anti-BALB/c CTL response coincides with that of a number of B-cell abnormalities but is shown in experiments with-suppressed NZB mice to be independent of B-cell dysfunction. Studies with (NZB x B10.D2)F1 + B10.D2 mice demonstrated that the anti-BALB/cCTL response to antigens coded for outside ofQa-1 is governed by at least two genes. Finally, it is shown that another conventionallyH-2-restricted response, that to TNP-modified isologous cells, is neither significantly cross-reactive nor markedly elevated in NZB mice. — The foregoing observations suggest that some subsets of NZB T lymphocytes are intrinsically abnormal. The possibilities that the apparent hyperreactivity of NZB CTL precursors, evidenced in the response to BALB/c cells, is primary or results from the secondary effects of excess T-cell help are discussed. 相似文献
4.
Structure function analysis of theH-2 Ab
p
gene 总被引:1,自引:0,他引:1
Jonathan A. Harton Wayne Litaker Jeffrey A. Frelinger Gail A. Bishop 《Immunogenetics》1991,34(6):358-365
The gene encoding the H-2A
p
class II chain was isolated from a B10.P genomic library and sequenced. This gene was also used to construct transfectants of the CH12 lymphoma clone CH12.LX, which express the Abp gene product in association with the endogenous A
k
chain. We present here the first report of the complete nucleotide coding sequence of Abp. The predicted amino acid sequence of Abp reveals only five residues different from Ab q, four of which are present in the mature peptide. These four amino acid changes could account for the differential susceptibility of H-2 q vs H-2 p mice to the development of collagen-induced arthritis (CIA). Antibodies specific for the transfected Abp protein induce CH 12. LX cells to secrete immunoglobulin in the presence of antigen. Comparison of the amino acid sequence with other A
chains that have been tested in signal transduction experiments suggests that amino acid 9 may be important to the signaling ability of class II A molecules. 相似文献
5.
Unexpected complexity of the dm1 mutation revealed in the structure of three H-2D/L-related antigens
Pamela H. Wilson Roderick Nairn Stanley G. Nathenson Duane W. Sears 《Immunogenetics》1982,15(3):225-237
The H-2Ldm1 and H-2Ddm1 MHC antigens of the B10.D2 (H-2
dm1
) mutant mouse strain (formerly known as M504 or H-2
da
) have been compared to the H-2Ld and H-2Dd antigens of the B10.D2 (H-2
d
) mouse strain. Ldm1 and Ld are 45 000 Mr antigens and both are reactive with anti-H-2.28 (k/r anti-h2) serum and unreactive with anti-H-2.4 (k/b anti-a) serum which detects private determinants of the Ddm1 and Dd antigens. However, the tryptic peptide compositions of these two antigens are different and, based on the number of major tryptic peptides which coelute during ion-exchange chromatography, the estimated peptide homology between Ldm1 and Ld is 80 percent. A newly defined antigen (Mr = 39 000), designated gp39dm1, was found in glycoprotein extracts of the dm1 strain but not of the d strain. This antigen coprecipitates with Ldm1 but does not coprecipitate with Ddm1 indicating that it lacks the H-2.4 determinant. In comparison with Ldm1, gp39dm1 appears to contain far fewer Arg and Lys residues and is most likely not a simple proteolytic fragment of Ldm1. Finally, peptide maps of the Ddm1 antigen show that the majority of its Arg peptides are identical to Dd Arg peptides, whereas at least five of its Lys peptides and three of its Arg peptides correspond not to Dd peptides but to Ld and Ldm1 peptides. These data raise the possibility that the Ddm1 antigen is a hybrid molecule and they have also revealed an unexpected level of complexity in the dm1 mutant phenotype. 相似文献
6.
Izumi Nakashima Tomoaki Yoshida Takashi Yokochi Kimiko Ohashi Fumihiko Nagase Ken-ichi Isobe Yoshinori Hasegawa Ko-ichi Ando Reiko Inagi 《Immunogenetics》1986,24(1):32-40
Immunogenicity for T cell-independent B-cell response assessed by splenic plaque-forming cell (PFC) response and cell-surface expression measured by laser flow cytometry of various class I H-2 antigens on mouse red blood cells (RBC) were compared. It was found that the order of magnitude of both immunogenicity and cell-surface expression on RBC is H-2Dd H-2Db > H-2Kd, H-2Kb. Furthermore, H-2d public antigens and H-2Ld antigens were neither immunogenic nor easily demonstrable on RBC. These findings contrasted with poor immunogenicity for PFC response (Nakashima et al. 1982, 1983) and proportionally strong expression of H-2 antigens on lymphoid cells. Immunogenicity and cell-surface expression of H-2Dd antigen on RBC were not shown to be controlled by the action of genes outside H-2D. It was therefore suggested that a number of H-2 antigens, including H-2Kd private, H-2Kb private, and H-2d public specificities are at least functionally defective on RBC. This is possibly due to the structural characteristics of the antigens. Since immunogenicity and cell-surface expression were in parallel, the expression of H-2 antigens on RBC must be dictated by a subset of B cells whose activity was assessed by PFC response. This finding supports the view that the H-2 molecules display a new category of activity which is different from their ability to activate T cells and depends on their expression on RBC. 相似文献
7.
Biochemical documentation of allelic variation of the I-E antigens of the d,k, p,r, and u haplotypes 总被引:1,自引:0,他引:1
John M. Kupinski Pamela A. Gamble Chella S. David Dr. John H. Freed 《Immunogenetics》1982,16(5):393-405
The extent of allelic variation of the E and E
polypeptide chains of the I-E antigens from the H-2>
d
,H-2
k
, H-2
p
, H-2
r
, and H-2
u
haplotypes is described. E and E
chains were individually labeled with arginine or lysine and compared by tryptic peptide analysis. The results indicate minimum variability among the E polypeptides encoded by the d, k, p, and r haplotypes. However, the E
u
chain differed significantly from the other allelic E gene products. On the other hand, the E
alleles demonstrated substantial variability with the E
d
being notably less similar to the other alleles than they are to each other. These findings are consistent with a number of observations regarding the serology and functions of the I-E antigens.Abbreviations MHC
major histocompatibility complex
- NMS
normal mouse serum
- NP-40
Nonidet P-40
- NTS
0.25% NP-40, 10 mM Tris-Cl, 0.15 M NaCl (pH 7.4)
- SDS
sodium dodecylsulfate
- SDS-PAGE
polyacrylamide gel electrophoresis in the presence of SDS 相似文献
8.
A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with the known sequence of H-2Db in 141 of 141 positions available for comparison. The clone, therefore, is believed to code for the H-2Db transplantation antigen. The cDNA insert of pH203 contains the coding region for residues 82 through the carboxy-terminus of H-2Db, and includes 476 nucleotides of the 3-untranslated sequence. Comparison between the H-2Db cDNA clone and a previously isolated H-2Kb cDNA clone shows homologies of 83% and 91% at the amino acid and nucleotide levels, respectively. Analysis of DNA sequences at the 3-coding and untranslated regions suggests that the mRNAs of H-2Kb and H-2Db are spliced differently at their 3-coding ends. 相似文献
9.
David E. Harrison 《Immunogenetics》1987,26(3):123-129
A semiquantitative assay was developed and used to measure the effects of immune responses against 16 independent non-H-2 antigenic loci on erythropoietic stem cells. The assay compares repopulation in genetically anemic WBB6F1-W/W
recipients that have normal immune responses, and in lethally irradiated WBB6F1 +/+ mice whose immune responses are suppressed by the irradiation. The differences in repopulating ability between these two types of recipients measure how immune responses affect erythropoietic stem cells. Stem cell repopulating abilities for the cells with antigens specified by the Thy-1, H-1, H-24, Ly-1, H-37, and H-17 loci were affected slightly, if at all. Repopulating abilities were moderately reduced by responses against antigens specified by H-15, 16, Ea-2, and Ly-2, 3 loci, and against the differences between the B6 and B10 genotypes, although marrow of these types cured W/W
recipients. A surprising result occurred for the antigen specified by the H-8 locus, in which immune responses strongly reduced repopulating abilities, although this type of marrow cell cured W/W
recipients. A comparison of these results with skin graft survival times suggests that the antigens specified by the H-17 and H-24 loci are strongly immunogenic on skin but not on marrow stem cells, while those specified by the H-12 and H-8 loci are strongly immunogenic on marrow stem cells but not on skin. 相似文献
10.
Mixed lymphocyte reaction (MLR) stimulation by purified T and B lymphocytes and thymocytes was studied. The MLR gene products involved were localized to theH-2 complex by the use of congenic mice differing atH-2, and to loci within theH-2 complex through the use of congenic mice bearing recombinant chromosome 17. Stimulation by T cells was investigated in detail. The role of small amounts of contaminating B lymphocytes, and that of backstimulation, was found to be of minor importance. T cells and thymocytes stimulated as well as or better than B cells in combinations differing in theI, S, and possibly parts of theD end, thus suggesting that these genetic regions control cell-surface products expressed on both T and B lymphocyte populations.Abbreviations used in this paper are MLR
mixed lymphocyte reaction
- GVHR
graft-versus-host reaction
- CML
cell-mediated lympholysis
- Thy-1
the gene for the T-cell antigens, synonymous with
- Thy-1.1
synonym for
AKR
- Thy-1.2
synonym for
C3H
- MHC
major histocompatibility complex
-
Ir genes
immune response genes linked to the MHC
- LPS
E. coli 055.35 lipopolysaccharide
For the genetic nomenclature of theH-2 complex (H-2K, H-2D, I, S, D regions,Ia, etc.) see Kleinet al. 1974, and Shreffleret al. 1974. 相似文献
11.
The hybrids (the CANS lines) between inflammatory macrophages from C57BL/6N (B6) mice (H-2b) and BALB/c mouse (H-2d)-derived myeloma cell line NS1 in the early period after cell fusion showed no macrophage functions. However, most of the hybrids expressed these functions after prolonged cultivation accompanied with chromosome loss. In contrast, the hybrids initially displaying myeloma functions ( light chain production) lost this function when they exhibited macrophage functions. We studied the expression of cell-surface antigens in these hybrids and found that hybrids in the early period after cell fusion codominantly expressed both parental cell H-2 antigens (H-2Kb, H-2Kd, and H-2Dd) but not the H-2Db antigen. On the other hand, aged hybrids strongly expressed the H-2 d antigen but lacked the H-2Kb antigen. Alternatively, these aged hybrids with macrophage functions expressed antigen(s) as detected with antiaged CANS-196 cell sera and asialo GM1 antigen, both of which were thought to be found exclusively on macrophages. Thus, the expression of cell-surface antigens in these hybrids was greatly altered after cell fusion. 相似文献
12.
We have studied the influence of DBA/2 non-H-2 antigens on the lethal graft-versus-host reaction (GVHR) developed across an H-2 barrier. (DBA/2 x B10.D2)F1 x B10.D2 (H-2
d) backcross (BC) mice were typed for their allelic constitution at nine genetically independent chromosome markers and used as individual cell donors simultaneously for two to three (DBA/2 X B10.D2)F1 recipients incompatible for DBA/2 non-H-2 antigens alone and two to three (DBA/2 x B10.BR)F1 recipients incompatible for DBA/2 non-H-2 antigens and H-2k. The results showed that, when compared with that developed in a control group incompatible for H-2
kalone [B10.D2(B10.D2xB10.BR)F1], the GVHR mortality seen in the presence of an additional incompatibility for DBA/2 non-H-2 antigens [(DBA/2 X B10.BR)F1recipients] is significantly delayed but only in female mice. An analysis of individual BC donors indicated that this protective effect of DBA/2 non-H-2 antigens correlates with incompatibility for gene(s) linked to the Pgm-1 chromosome marker. In contrast, incompatibility for gene(s) linked to Mod-1 and Es-3 markers accelerates GVHR mortality, but only in male mice. Finally, the results obtained with (DBA/2 x B10.D2)F1 and (DBA/2 x B10.BR)F1 recipients were compared; they showed that the intensity of the GVHR developed by cells from individual BC donors against a given set of DBA/2 non-H-2 antigens correlates well with that developed by the same BC donor against the same set of non-H-2 antigens plus H-2k. We conclude that certain non-H-2 genes (and antigens) can modulate the intensity of the GVHR developed across an H-2 barrier. The number of such genes is probably great; their effects are strong and complex, and can be sex-dependent. 相似文献
13.
Mayumi Nakagawa Richard A. Zeff Steven S. Geier Jeffrey A. Bluestone Stanley G. Nathenson 《Immunogenetics》1986,24(6):381-385
A cell-surface-associated variant H-2K product was expressed by an Abelson virus-induced pre-B-cell line after chemical mutagenesis with ethyl methane sulfonate. The variant cell line (R8.313) was previously demonstrated to have altered allodeterminants in Kb as demonstrated by both Kb-specific monoclonal antibody binding and alloreactive cytotoxic T lymphocyte (CTL) cytolysis. The mutant H-2K
b gene from R8.313 was cloned and characterized in detail. DNA sequence analysis of the region of the gene corresponding to the three extracellular domains identified a single point mutation resulting in a leucine-to-phenylalanine substitution at amino acid residue 82. The site of mutation within the 1 domain was confirmed by oligonucleotide hybridization analysis. Mouse L-cell fibroblasts transfected with the mutant gene were recognized with the same monoclonal antibody binding and CTL lytic pattern as the R8.313 cell line, confirming that the altered phenotype of the mutant cell line was due to a point mutation in the H-2K
b gene. These data further extend the hypothesis that the region of amino acid residues 70–90 in the 1 domain is important in the formation of both antibody and CTL-defined recognition structures on major histocompatibility complex class I molecules. 相似文献
14.
The major goal of these studies is to more fully assess the polymorphism of the hemopoietic histocompatibility (Hh) genetic system. H-2 homozygosity is required for optimal immunogenicity of bone marrow cell (BMC) grafts, and hybrid resistance to grafts of parental strain BMC by irradiated H-2 heterozygous F1 hybrid mice suggests that Hh-1 antigens are inherited recessively. The Hh-1 antigens are also expressed on other normal hematopoietic cells and lymphoid tumors, and natural killer cells are the effectors which mediate the elimination of BMC grafts in an Hh-specific manner. Previous studies have demonstrated three different antigens mapping to the Hh-1 locus near H-2D. We test the expression of Hh-1 on BMC of all nonrecombinant H-2 haplotypes of independent origin and H-2
j
, a presumed natural recombinant. Hh-1 typing is based on the pattern of growth and rejection in a panel of hosts. F1 hybrids with H-2
b
, H-2
d
, and H-2
k
are produced and used as donors and hosts to confirm the phenotype. Grafts of b-, d-, and j-haplotype marrow serve as prototypical examples of determinants that are provisionally designated as 1, 2, and 3, respectively. We describe a new determinant, 4, in the k haplotype. It is non-codominantly expressed, maps to H-2D, and is also expressed on H-2b BMC. NZW, H-2Z grafts exhibit a phenotype similar to k, but express a unique determinant 5 which can be distinguished from determinant 4. This additional determinant is also expressed by the b haplotype. The d, f, and p haplotypes all express determinant 2, and grafts of j-haplotype marrow are found to express determinants 2 and 5 in addition to determinant 3. The q and r haplotypes are null for all known determinants. Finally, we describe a phenotype which is a new combination of previously described determinants: s-haplotype grafts express determinants 1, 2, and 4. The polymorphism of Hh-1 detected thus far consists of seven alleles which are combinations of five distinct determinants. 相似文献
15.
Scheherazade Sadegh-Nasseri Dale E. Kipp Benjamin A. Taylor Alexander Miller Eli Sercarz 《Immunogenetics》1984,20(5):535-546
Genes outside of the mouse major histocompatibility complex (H-2) were found to be capable of specifically reversing the previously described nonresponsiveness to hen egg-white lysozyme (HEL) owing to H-2
b
immune response (Ir) genes. C3H.SW, BALB.B, and C57L, all of the H-2
b
haplotype, showed responsiveness to HEL, but not to human lysozyme (H UL). Mapping of the reversing gene(s) was attempted by testing H-2
b
recombinant inbred (RI) strains of mice carrying C3H, BALB, and C57L non-H-2
b
genes. Analysis of the strain distribution pattern of responsiveness with both CXB and BXH RI strains was consistent with the location of the responsible site within the H-3 region on chromosome 2. The anti-HEL proliferative responsiveness in two H-3 congenic strains of mice, B10.C(28NX)
SN
and B10.C-H-3
cH-3
a
, that have BALB/c genes within the H-3 region confirmed the mapping, as well as localized the reversing gene(s) near the Ir-2 gene. The data are discussed with regard to the site of expression of the reversing gene(s) and its mechanism of action.Abbreviations used in this paper MHC
major histocompatibility complex
- HEL
hen egg-white lysozyme
-
Ir
immune response gene
- HUL
human lysozyme
- SDP
strain distribution pattern
- PFC
plaque-forming cells;
2
m,
2-microglobulin
- CFA
complete Freund's adjuvant
- PT-LN
parathymic lymph nodes
- RI
recombinant inbred mice 相似文献
16.
Françoise Albert Michel Buferne Claude Boyer Anne-Marie SchmittVerhulst 《Immunogenetics》1982,16(6):533-549
To study the interactions between T cells and class I MHC products, we developed in vitro a T-cell line reactive to H-2Kb stimulating cells and derived T-cell clones from it. Although the T-cell line could proliferate in the absence of exogeneous T-cell growth factors when stimulated with H-2Kb spleen cells, each of the derived T-cell clones required both H-2Kb stimulating cells and an external source of T-cell growth factor for its propagation. Each of the T-cell clones was also cytolysic for H-2Kb target cells. Such T-cell clones allowed the comparison of the antigenic requirements for proliferation and cytolysis. By using H-2K
b
mutant mice, we found that while the original anti-H-2Kb T-cell line reacted with each of the six mutants tested, the individual T-cell clones could be distinguished in terms of their reactivity pattern. Similar fine specificity patterns were found when H-2K
b
mutant cells were used as stimulating or target cells for any given T-cell clone. Each of the three monoclonal H-2Kb-specific antibodies reacting with different epitopes of the H-2Kb molecule totally inhibited H-2Kb-induced proliferation and lysis by the T-cell clones. Further blocking studies involved use of Fab antibody fragments and definition of their reactivity on cells from the H-2K
b
mutants. We concluded that: (1) blocking with a monoclonal antibody does not prove identity of alloantigens recognized by the T-cells and the antibody; (2) a monoclonal antibody could either block or not block H-2Kb-CTL interactions depending on structural variations of the H-2Kb molecule not affecting the CTL-H-2Kb functional interaction; (3) blocking one type of H-2Kb-T-cell interaction (induction of proliferation) always affects the other type (cytolysis).Abbreviations used in this paper MHC
major histocompatibility complex
- CTL
cytotoxic
- T
lymphocytes
- Th
T helper cells
- PMA
4-phorbol 12-myristate 13-acetate
- Con A
Concanavalin A
- LPS
E. coli lipopolysaccharide
- SCA
Con A stimulated rat spleen-cells supernatant
- SBD
B6 anti-DBA/2 mixed lymphocyte culture supernatant
- TCGF
T-cell growth factors
- IL-2
interleukin 2
- mAb
monoclonal antibody
- FCS
fetal calf serum
- PBS
phosphate buffered saline
- C
complement 相似文献
17.
Direct immunoprecipitation and two-dimensional (2D) gel electrophoresis have been used to identify and characterize genetic variation of theH-2K andH-2D regions. Using inbred strains of mice and alloantisera, haplotype-specific polypeptides were defined for five differentH-2 haplotypes. Specific immunoprecipitates prepared from strains of different haplotypes were applied to 2D gels in pairwise combinations to determine whether peptides specific to one haplotype can be distinguished from peptides specific to another. Those haplotype-specific peptides that migrate to unique positions on 2D gels with respect to the positions occupied by haplotype-specific peptides of another haplotype are useful as biochemical genetic markers. Cross-reactivity amongK- andD-region antigens of different haplotypes was identified on 2D gels and found to correlate well with existing data based on serological cross-reactivity. An anti-mouse 2-microglobulin serum was found to be a useful general reagent for immunoprecipitating haplotype-specific H-2 antigens to permit their visualization on 2D gels.Abbrevations used in this paper NP-40
nonidet P-40
- 2D
two-dimensional
- SDS
sodium dodecyl sulfate
- IEF
isoelectric focusing 相似文献
18.
Structural evidence that the small subunit found associated with the TL antigen is β
2-microglobulin
Kazushige Yokoyama Elisabeth Stockert Lloyd J. Old Dr. Stanley G. Nathenson 《Immunogenetics》1982,15(6):543-549
Comparative tryptic peptide mapping and partial amino-terminal primary sequence analysis of the light chain component associated with the TL antigens showed that the small subunit of TL was identical to the
2m light chain associated with the H-2K or D product of the same strain. Peptide comparison of the
2m from the Tla products of an A strain X-ray induced leukemia RADA1 (Tla
a) and of a C57BL/6 strain X-ray induced leukemia ERLD (Tla
b) showed differences to the extent of 25–35% in their peptides. This is consistent with previous results showing
2m allelic variations between these mouse strains. The data prove the structural identity of the
2m molecules from TL and H-2K, D antigens as well as reveal the strain specific polymorphism of the
2m associated with these products. 相似文献
19.
In preparation for functional analyses, a study of the binding of H-2Kb-specific monoclonal antibodies (mAb) to formaldehyde (FOR)-fixed H-2b spleen or tumor cells revealed that three of nine mAb tested had lost reactivity with the FOR-fixed cells, whereas the reactivity of the other mAb generally did not diminish. Comparison of the reactivity of these mAb on untreated H-2K
bm
mutant cells and on FOR-treated H-2Kb cells suggests that for three mAb the total loss of reactivity on the latter could be a consequence of the alteration by FOR of lysine 89, which is substituted by alanine in mutant bm3. H-2KPb-specific alloreactive polyclonal or monoclonal CTL, all of which had retained reactivity with bm3 target cells, had also retained reactivity with FOR-fixed H-2b cells as indicated by cold target inhibition studies. The H-2Kb-specific CTL were probably reactive with conformational determinants of H-2Kb, which are dependent on the integrity of both the 1 and the 2 domains of the H-2Kb molecule. Results are compatible with FOR treatment selectively affecting a serological determinant in the 1 domain without affecting conformational-type CTL determinants.Abbreviations used in this paper CTL
cytotoxic T lymphocyte
- FOR
formaldehyde
- PBS
phosphatebuffered saline
- FCS
fetal calf serum
- mAb
monoclonal antibody
- TNBS
trinitrobenzene sulfonate 相似文献
20.
HLA-DR2-associated Dw subtypes correlate with RFLP clusters: most DR2 IDDM patients belong to one of these clusters 总被引:4,自引:0,他引:4
Nadine Cohen Chaim Brautbar Marie-Pierre Font Jean Dausset Daniel Cohen 《Immunogenetics》1986,24(2):84-89
The incidence of arthritis and the antibody response to mouse and to rat type 11 collagen after immunization with native rat type II collagen was studied in different mouse strains, including wild mouse-derived strains belonging to the H-2p/H-2q family. High serum levels of antibodies to mouse and rat type II collagen were seen only in H-2q mice, whereas mice belonging to the p, w3, w5, and w17 haplotypes displayed low type II collagen-specific antibody responses. Mice from three different H-2q-carrying strains (DBA/1, NFR/N, and B10.G) with different non-major histocompatibility complex genes were all susceptible to collagen arthritis, but they displayed a varying incidence of arthritis and varying clinical features. No arthritis was seen in non-H-2q mice, except in the B10.CAS2 strain where a few mice developed arthritis despite very low serum levels of type II collagen-specific antibodies. We conclude that small differences in the A chain of class II transplantation antigens are of importance for the development of arthritis and for the stimulation of a high response after immunization with type 11 collagen.Abbreviations used in this paper ELISA
enzyme-linked immunosorbent assay
- PBS
phosphate-buffered saline
- Ig
immunoglobulin
- MHC
major histocompatibility complex 相似文献